MC05g1586 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g1586
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	short-chain dehydrogenase reductase 2a-like
Location	MC05: 19586734 .. 19588644 (+)
RNA-Seq Expression	MC05g1586
Synteny	MC05g1586

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCAAAACCAAATCTAAATTGTATATTGAATGATGAGATGGAGACAAATTAGGCAATGGAAGGGAGAGGGACGAGGGGCAAGTGAAACGCCACTATGTAGGAAGCTGCCTTGAACTACCCAACAACTTTTCTCAAATTCTTACTAAAATAGAATAAAATAAAATAACTTTTCTCAAAATCTCTCCCTATAAAATCCCCTTCTTTGCGCAATTGTTGCTGCTACCGCAAAATCTCGCTCTCTGATCAGCCATGACTGCCCAAGTAATGCCGGAGCAACAGCAACCCCTCCACGCCATTCATCTCTTACCAAGGGATTTGGATAAATCCCCTCCCTCCCACAAAAGGTATTAATTAATTCATACAATTAAGTACAACACTATTAATTGTATTATTTTATATTTAATTAATGTGATCGTCGATTGTTTTCAGGCTGGAGGGAAAAGTGGCGATCGTAACGGGCGGCGCGAAGGGGATCGGAGAGGCGACGGTGAGGCTGTTCGCGAAGCACGGAGCGAGAGTGGTGATCGCCGACGTGGAGGATCTACTCGGGGCGGCGTTGGCGGACACGCTGTCACCATGCCAGGTCACGTTCGTGCACTGCGACGTCAGCTCGGAAGAGGACGTGGAGAATGCAATAAACTCGACGATACGTCGTTACGGGCAGGTGGACGTGATATTCAACAACGCCGGAGTGCTGGGGAACCAGTCGCAGCGGAAGAGCATAGTGGACTTTGACCCGGAGGAGTTCGAGCGCGTGATGCGCGTGAACGTGAAGGGCGTGGCGTTGGGGATAAAGCACGCGGCGCGCGTGATGATTCCGAGGGGCAGCGGTTGCATAATATCGACGGCCAGCGTGGCGGGGGTTCTGGGGGGGCTGGGCCCGCACGCGTACACGGCGTCGAAGCACGCCATTGTGGGGCTCACAAAGAACACCGCCTGCGAGCTCGGCCGCTACGGGATACGGGTCAACTGCATTTCGCCGTTCGGAGTGGCCACGTCGATGCTCGTGAACGCGTGGAGGAGCCACGACGACGACGACGAAGAAGAAGAAGAGGGCATGATTCCGTCGGCTCAGGAAGTTGAGAAAATGGAGGATTTCGTTAGGGGTTTGGCCAATTTGAAGGGGCCCACATTGAGGGCGAAGGATATTGCAGAGGCGGCGCTGTATTTGGCCTCCGATGAATCCAAGTACATCAGCGGCCATAACCTCGTCGTTGATGGCGGAATCACCACTTCCAGAAATTGTGTTGGCTTGTAATTTTCTTTTTCTATCCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAGGTGTCAGTGGGGGAAAGAAAAAAAAAAAACTCCCTCATATGCCTGTTGTTTGTTTCTACTTTCTAGTAGGATTTACGATTTAGTTTGTTATTTATTATTTGATCTACAATAGCTGAATATATTAGTTGTTCTATTCTACAGCTGCTTGTAGTTATCTTTCATAACTCCTAAAACTTTTGAATTCATGTTTTCGTTTTAATTTAAGTACTCTGCTTGAAATTATTGATAAAACTGTTACCTCAAATTCGTGGGTCGAATTTGTTTAATGCATCTAACGATCAAAATGGTTAAAAACACATTTATTTGCTCAGGTAATTTTTTTAAAATAACGAATTAGTCTATAAACTTCTAATAGTTTAATTTTGGTAGGACTGTGTCAATATCTAACATAAATATGATGTGGGATGCCAGTTGCTTGGACATACTTTAACCAAAAAAAATAGGACAAATAAAATGCCTCCCAGTCCCCAACTTACAATCGACCATCGACGGTAAAGCTGGTCGTCGAAAATGGTCTTTATTATTTATTTACAGTGATG

mRNA sequence

GTCAAAACCAAATCTAAATTGTATATTGAATGATGAGATGGAGACAAATTAGGCAATGGAAGGGAGAGGGACGAGGGGCAAGTGAAACGCCACTATGTAGGAAGCTGCCTTGAACTACCCAACAACTTTTCTCAAATTCTTACTAAAATAGAATAAAATAAAATAACTTTTCTCAAAATCTCTCCCTATAAAATCCCCTTCTTTGCGCAATTGTTGCTGCTACCGCAAAATCTCGCTCTCTGATCAGCCATGACTGCCCAAGTAATGCCGGAGCAACAGCAACCCCTCCACGCCATTCATCTCTTACCAAGGGATTTGGATAAATCCCCTCCCTCCCACAAAAGGCTGGAGGGAAAAGTGGCGATCGTAACGGGCGGCGCGAAGGGGATCGGAGAGGCGACGGTGAGGCTGTTCGCGAAGCACGGAGCGAGAGTGGTGATCGCCGACGTGGAGGATCTACTCGGGGCGGCGTTGGCGGACACGCTGTCACCATGCCAGGTCACGTTCGTGCACTGCGACGTCAGCTCGGAAGAGGACGTGGAGAATGCAATAAACTCGACGATACGTCGTTACGGGCAGGTGGACGTGATATTCAACAACGCCGGAGTGCTGGGGAACCAGTCGCAGCGGAAGAGCATAGTGGACTTTGACCCGGAGGAGTTCGAGCGCGTGATGCGCGTGAACGTGAAGGGCGTGGCGTTGGGGATAAAGCACGCGGCGCGCGTGATGATTCCGAGGGGCAGCGGTTGCATAATATCGACGGCCAGCGTGGCGGGGGTTCTGGGGGGGCTGGGCCCGCACGCGTACACGGCGTCGAAGCACGCCATTGTGGGGCTCACAAAGAACACCGCCTGCGAGCTCGGCCGCTACGGGATACGGGTCAACTGCATTTCGCCGTTCGGAGTGGCCACGTCGATGCTCGTGAACGCGTGGAGGAGCCACGACGACGACGACGAAGAAGAAGAAGAGGGCATGATTCCGTCGGCTCAGGAAGTTGAGAAAATGGAGGATTTCGTTAGGGGTTTGGCCAATTTGAAGGGGCCCACATTGAGGGCGAAGGATATTGCAGAGGCGGCGCTGTATTTGGCCTCCGATGAATCCAAGTACATCAGCGGCCATAACCTCGTCGTTGATGGCGGAATCACCACTTCCAGAAATTGTGTTGGCTTGTAATTTTCTTTTTCTATCCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAGGTGTCAGTGGGGGAAAGAAAAAAAAAAAACTCCCTCATATGCCTGTTGTTTGTTTCTACTTTCTAGTAGGATTTACGATTTAGTTTGTTATTTATTATTTGATCTACAATAGCTGAATATATTAGTTGTTCTATTCTACAGCTGCTTGTAGTTATCTTTCATAACTCCTAAAACTTTTGAATTCATGTTTTCGTTTTAATTTAAGTACTCTGCTTGAAATTATTGATAAAACTGTTACCTCAAATTCGTGGGTCGAATTTGTTTAATGCATCTAACGATCAAAATGGTTAAAAACACATTTATTTGCTCAGGTAATTTTTTTAAAATAACGAATTAGTCTATAAACTTCTAATAGTTTAATTTTGGTAGGACTGTGTCAATATCTAACATAAATATGATGTGGGATGCCAGTTGCTTGGACATACTTTAACCAAAAAAAATAGGACAAATAAAATGCCTCCCAGTCCCCAACTTACAATCGACCATCGACGGTAAAGCTGGTCGTCGAAAATGGTCTTTATTATTTATTTACAGTGATG

Coding sequence (CDS)

ATGACTGCCCAAGTAATGCCGGAGCAACAGCAACCCCTCCACGCCATTCATCTCTTACCAAGGGATTTGGATAAATCCCCTCCCTCCCACAAAAGGCTGGAGGGAAAAGTGGCGATCGTAACGGGCGGCGCGAAGGGGATCGGAGAGGCGACGGTGAGGCTGTTCGCGAAGCACGGAGCGAGAGTGGTGATCGCCGACGTGGAGGATCTACTCGGGGCGGCGTTGGCGGACACGCTGTCACCATGCCAGGTCACGTTCGTGCACTGCGACGTCAGCTCGGAAGAGGACGTGGAGAATGCAATAAACTCGACGATACGTCGTTACGGGCAGGTGGACGTGATATTCAACAACGCCGGAGTGCTGGGGAACCAGTCGCAGCGGAAGAGCATAGTGGACTTTGACCCGGAGGAGTTCGAGCGCGTGATGCGCGTGAACGTGAAGGGCGTGGCGTTGGGGATAAAGCACGCGGCGCGCGTGATGATTCCGAGGGGCAGCGGTTGCATAATATCGACGGCCAGCGTGGCGGGGGTTCTGGGGGGGCTGGGCCCGCACGCGTACACGGCGTCGAAGCACGCCATTGTGGGGCTCACAAAGAACACCGCCTGCGAGCTCGGCCGCTACGGGATACGGGTCAACTGCATTTCGCCGTTCGGAGTGGCCACGTCGATGCTCGTGAACGCGTGGAGGAGCCACGACGACGACGACGAAGAAGAAGAAGAGGGCATGATTCCGTCGGCTCAGGAAGTTGAGAAAATGGAGGATTTCGTTAGGGGTTTGGCCAATTTGAAGGGGCCCACATTGAGGGCGAAGGATATTGCAGAGGCGGCGCTGTATTTGGCCTCCGATGAATCCAAGTACATCAGCGGCCATAACCTCGTCGTTGATGGCGGAATCACCACTTCCAGAAATTGTGTTGGCTTGTAA

Protein sequence

MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVGL

Homology

[Close]

BLAST of MC05g1586 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 2.3e-109
Identity = 208/312 (66.67%), Postives = 249/312 (79.81%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           M AQV+ E Q   H++H    +   +    KRLEGKVAI+TGGA GIG+ATV LFA+HGA
Sbjct: 1   MPAQVIAE-QTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQ----VTFVHCDVSSEEDVENAINSTIRRYGQVDVIFN 120
            VVIADV+++ G++LA +LS  +    V F+ CDVS E DVEN +N T+ RYG++D++FN
Sbjct: 61  TVVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFN 120

Query: 121 NAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRG-SGCIISTASVA 180
           NAGVLG+Q + KSI+DFD +EF+ VMRVNV+GV LG+KH AR MI RG  GCIISTASVA
Sbjct: 121 NAGVLGDQKKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVA 180

Query: 181 GVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDD 240
           GV+GG+GPHAYTASKHAIVGLTKN ACELG+YGIRVNCISPFGVATSMLVNAWR     D
Sbjct: 181 GVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGD 240

Query: 241 EEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVD 300
            E++        +VE+ME+FVR LANLKG TLRA DIAEAALYLASDESKY++GHNLVVD
Sbjct: 241 VEDD--------DVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVVD 300

Query: 301 GGITTSRNCVGL 308
           GG+TT+RNCVGL
Sbjct: 301 GGVTTARNCVGL 303

[Close]

BLAST of MC05g1586 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 5.2e-101
Identity = 194/290 (66.90%), Postives = 230/290 (79.31%), Query Frame = 0


Query: 28  PSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFV 87
           P  KRL+GKVAIVTGGA+GIGEA VRLFAKHGARVVIAD++D  G ALA  L P QV+FV
Sbjct: 48  PMPKRLDGKVAIVTGGARGIGEAIVRLFAKHGARVVIADIDDAAGEALASALGP-QVSFV 107

Query: 88  HCDVSSEEDVENAINSTIRRY-GQVDVIFNNAGVLGNQSQ-RKSIVDFDPEEFERVMRVN 147
            CDVS E+DV  A++  + R+ G++DV  NNAGVLG Q++  +SI+ FD  EF+RV+RVN
Sbjct: 108 RCDVSVEDDVRRAVDWALSRHGGRLDVYCNNAGVLGRQTRAARSILSFDAAEFDRVLRVN 167

Query: 148 VKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELG 207
             G ALG+KHAAR M PR +G I+S ASVA VLGGLGPHAYTASKHAIVGLTKN ACEL 
Sbjct: 168 ALGAALGMKHAARAMAPRRAGSIVSVASVAAVLGGLGPHAYTASKHAIVGLTKNAACELR 227

Query: 208 RYGIRVNCISPFGVATSMLVNAWR-SHDDDDEEEEEGM-------IPSAQEVEKMEDFVR 267
            +G+RVNC+SPFGVAT ML+NAWR  HDD   + +  +       +PS QEVEKME+ VR
Sbjct: 228 AHGVRVNCVSPFGVATPMLINAWRQGHDDATADADRDLDLDLDVTVPSDQEVEKMEEVVR 287

Query: 268 GLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVGL 308
           GLA LKGPTLR +DIAEA L+LASDE++YISGHNLVVDGG+TTSRN +GL
Sbjct: 288 GLATLKGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTTSRNLIGL 336

[Close]

BLAST of MC05g1586 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.1e-70
Identity = 136/275 (49.45%), Postives = 192/275 (69.82%), Query Frame = 0


Query: 31  KRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCD 90
           +RLEGKVA++TGGA GIGE T +LF++HGA+V IADV+D LG ++ + +     T++HCD
Sbjct: 13  RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCD 72

Query: 91  VSSEEDVENAINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVA 150
           V++E+ V+NA+++T+  YG++D++F+NAG+  +   R  I+D +  +FERV  VNV GV 
Sbjct: 73  VTNEDGVKNAVDNTVSTYGKLDIMFSNAGI--SDPNRPRIIDNEKADFERVFSVNVTGVF 132

Query: 151 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 210
           L +KHAARVMIP  SG IISTAS++  +GG   HAY  SKHA++GLT+N A ELG++GIR
Sbjct: 133 LCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIR 192

Query: 211 VNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAK 270
           VNC+SPFG+ T++     +     +EEE E +I  A              NLKGP    +
Sbjct: 193 VNCLSPFGLPTAL---GKKFSGIKNEEEFENVINFA-------------GNLKGPKFNVE 252

Query: 271 DIAEAALYLASDESKYISGHNLVVDGGITTSRNCV 306
           D+A AALYLASDE+KY+SGHNL +DGG +   + +
Sbjct: 253 DVANAALYLASDEAKYVSGHNLFIDGGFSVCNSVI 269

[Close]

BLAST of MC05g1586 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.4e-66
Identity = 136/280 (48.57%), Postives = 181/280 (64.64%), Query Frame = 0


Query: 32  RLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTL-SPCQVTFVHCD 91
           RLEGKVA+VTGGA GIGE+  RLF +HGA++ I DV+D LG  ++  L       + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 92  VSSEEDVENAINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVA 151
           V+ E+DV  A++ T  +YG +D++ NNAG+ G++     I D D  EF++V  +NV GV 
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKV--IDIRDADFNEFKKVFDINVNGVF 121

Query: 152 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 211
           LG+KHAAR+MIP+  G I+S ASV+ V+ G GPH YT +KHA+VGLTK+ A ELGR+GIR
Sbjct: 122 LGMKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIR 181

Query: 212 VNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMED-------FVRGLANLK 271
           VNC+SP+ V T +                   +P   E E  ED       FVR  ANLK
Sbjct: 182 VNCVSPYAVPTRL------------------SMPYLPESEMQEDALRGFLTFVRSNANLK 241

Query: 272 GPTLRAKDIAEAALYLASDESKYISGHNLVVDGGITTSRN 304
           G  L   D+AEA LYLA++ESKY+SG NLV+DGG + + +
Sbjct: 242 GVDLMPNDVAEAVLYLATEESKYVSGLNLVIDGGFSIANH 261

[Close]

BLAST of MC05g1586 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 1.9e-66
Identity = 142/277 (51.26%), Postives = 182/277 (65.70%), Query Frame = 0


Query: 31  KRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCD 90
           ++L GKVA++TGGA GIG  T RLF KHGARVV+AD++D LGA+L   L P   ++VHCD
Sbjct: 13  RKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCD 72

Query: 91  VSSEEDVENAINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVA 150
           V++E DV  A++  + R+G++DV+FNNAGV G    R S  +   E+FERV+ VN+ G  
Sbjct: 73  VTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMS--ECTKEDFERVLAVNLVGPF 132

Query: 151 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 210
           LG KHAARVM P   G IISTAS++  + G   HAYT SKHA+VG T+N A ELGR+GIR
Sbjct: 133 LGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIR 192

Query: 211 VNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGP-TLRA 270
           VNC+SP GVAT  L  A    DD                E +E  +   ANLKG   L+A
Sbjct: 193 VNCVSPAGVATP-LARAAMGMDD----------------EAIEAIMANSANLKGAGALKA 252

Query: 271 KDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVG 307
            DIA AAL+LASD+ +Y+SG NL VDGG++   +  G
Sbjct: 253 DDIAAAALFLASDDGRYVSGQNLRVDGGLSVVNSSFG 270

[Close]

BLAST of MC05g1586 vs. NCBI nr
Match: XP_022934318.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 488 bits (1257), Expect = 5.33e-172
Identity = 260/308 (84.42%), Postives = 278/308 (90.26%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + IHLL RD   SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQALQSN-IHLLGRD--NSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  V+FVHCDVSSEEDVEN I+ST+ R+GQVD+IFNNAGV
Sbjct: 61  KVVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGV 120

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+  KSIVDFDP EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 121 LGNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D+++E  
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECM 240

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IPS  EV+KME+FVRGL+NLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGIT
Sbjct: 241 NLGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 300

Query: 301 TSRNCVGL 307
           TSRNC+GL
Sbjct: 301 TSRNCIGL 305

[Close]

BLAST of MC05g1586 vs. NCBI nr
Match: XP_022983300.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita maxima])

HSP 1 Score: 484 bits (1247), Expect = 1.78e-170
Identity = 258/308 (83.77%), Postives = 277/308 (89.94%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + + LL RD   SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQALQTNIL-LLGRD--NSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  V+FVHCDVSSEEDVEN I+ST+ R+GQVD+IFNNAGV
Sbjct: 61  KVVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGV 120

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+  KSIVDFDP EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 121 LGNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D+++E  
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECM 240

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IPS  EV+KME+FVRGL+NLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGIT
Sbjct: 241 NLGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 300

Query: 301 TSRNCVGL 307
           TSRNC+GL
Sbjct: 301 TSRNCIGL 305

[Close]

BLAST of MC05g1586 vs. NCBI nr
Match: XP_023528551.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 488 bits (1256), Expect = 2.04e-170
Identity = 260/308 (84.42%), Postives = 278/308 (90.26%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + IHLL RD   SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 93  MTAQVLPEQALQSN-IHLLGRD--NSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 152

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  V+FVHCDVSSEEDVEN I+ST+ R+GQVD+IFNNAGV
Sbjct: 153 KVVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGV 212

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+  KSIVDFDP EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 213 LGNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 272

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D+++E  
Sbjct: 273 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECM 332

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IPS  EV+KME+FVRGL+NLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGIT
Sbjct: 333 NLGIPSVGEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 392

Query: 301 TSRNCVGL 307
           TSRNC+GL
Sbjct: 393 TSRNCIGL 397

[Close]

BLAST of MC05g1586 vs. NCBI nr
Match: XP_004137687.1 (short-chain dehydrogenase reductase 2a [Cucumis sativus] >KGN58711.1 hypothetical protein Csa_002296 [Cucumis sativus])

HSP 1 Score: 483 bits (1243), Expect = 8.05e-170
Identity = 257/312 (82.37%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPS-HKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60
           MTAQV+PEQ    + IHLL RD   SPP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHG
Sbjct: 1   MTAQVLPEQSLQSN-IHLLVRDNSSSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60

Query: 61  ARVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAG 120
           A+VVIADVED+LG ALA+TLSP  V+FVHCDVSSE+DVEN +++T+  +GQVD+IFNNAG
Sbjct: 61  AKVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAG 120

Query: 121 VLGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVL 180
           VLGNQS   KSI+DFDP+EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVL
Sbjct: 121 VLGNQSNSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVL 180

Query: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEE 240
           GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+   D EEE
Sbjct: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRA---DVEEE 240

Query: 241 EEGM---IPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVD 300
           EE M   +PSA EV+KME+FVRGLANLKGPTL+ KDIA+AALYLASDESKYISGHNLVVD
Sbjct: 241 EECMNYGVPSAAEVDKMEEFVRGLANLKGPTLKPKDIAQAALYLASDESKYISGHNLVVD 300

Query: 301 GGITTSRNCVGL 307
           GG+TTSRNC+GL
Sbjct: 301 GGVTTSRNCIGL 308

[Close]

BLAST of MC05g1586 vs. NCBI nr
Match: XP_038905282.1 (short-chain dehydrogenase reductase 2a [Benincasa hispida])

HSP 1 Score: 482 bits (1241), Expect = 1.51e-169
Identity = 254/309 (82.20%), Postives = 281/309 (90.94%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHA-IHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60
           MTAQ++PEQ  PL   IHLL RD + SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHG
Sbjct: 1   MTAQLLPEQ--PLQTNIHLLGRD-NNSPSFHKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60

Query: 61  ARVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAG 120
           A+VVIADVED+LG  LA+TLSP  V+FVHCDVSSEEDVEN +++T+ R+GQVD+IFNNAG
Sbjct: 61  AKVVIADVEDVLGQTLAETLSPFPVSFVHCDVSSEEDVENLVSTTVCRHGQVDIIFNNAG 120

Query: 121 VLGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVL 180
           VLG+QS+ RKSI+DFDP+EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVL
Sbjct: 121 VLGSQSKARKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVL 180

Query: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEE 240
           GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D++++ 
Sbjct: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVDEEDQC 240

Query: 241 EEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGI 300
               +PSA EV+KME+FVRGLANLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGI
Sbjct: 241 MNFGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGI 300

Query: 301 TTSRNCVGL 307
           TTS NC+GL
Sbjct: 301 TTSNNCIGL 306

[Close]

BLAST of MC05g1586 vs. ExPASy TrEMBL
Match: A0A6J1F7B6 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC111441518 PE=4 SV=1)

HSP 1 Score: 488 bits (1257), Expect = 2.58e-172
Identity = 260/308 (84.42%), Postives = 278/308 (90.26%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + IHLL RD   SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQALQSN-IHLLGRD--NSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  V+FVHCDVSSEEDVEN I+ST+ R+GQVD+IFNNAGV
Sbjct: 61  KVVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGV 120

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+  KSIVDFDP EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 121 LGNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D+++E  
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECM 240

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IPS  EV+KME+FVRGL+NLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGIT
Sbjct: 241 NLGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 300

Query: 301 TSRNCVGL 307
           TSRNC+GL
Sbjct: 301 TSRNCIGL 305

[Close]

BLAST of MC05g1586 vs. ExPASy TrEMBL
Match: A0A6J1J5H2 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111481921 PE=4 SV=1)

HSP 1 Score: 484 bits (1247), Expect = 8.60e-171
Identity = 258/308 (83.77%), Postives = 277/308 (89.94%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + + LL RD   SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQALQTNIL-LLGRD--NSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  V+FVHCDVSSEEDVEN I+ST+ R+GQVD+IFNNAGV
Sbjct: 61  KVVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGV 120

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+  KSIVDFDP EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 121 LGNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D+++E  
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECM 240

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IPS  EV+KME+FVRGL+NLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGIT
Sbjct: 241 NLGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 300

Query: 301 TSRNCVGL 307
           TSRNC+GL
Sbjct: 301 TSRNCIGL 305

[Close]

BLAST of MC05g1586 vs. ExPASy TrEMBL
Match: A0A0A0LF57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1)

HSP 1 Score: 483 bits (1243), Expect = 3.90e-170
Identity = 257/312 (82.37%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPS-HKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60
           MTAQV+PEQ    + IHLL RD   SPP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHG
Sbjct: 1   MTAQVLPEQSLQSN-IHLLVRDNSSSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60

Query: 61  ARVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAG 120
           A+VVIADVED+LG ALA+TLSP  V+FVHCDVSSE+DVEN +++T+  +GQVD+IFNNAG
Sbjct: 61  AKVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAG 120

Query: 121 VLGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVL 180
           VLGNQS   KSI+DFDP+EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVL
Sbjct: 121 VLGNQSNSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVL 180

Query: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEE 240
           GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+   D EEE
Sbjct: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRA---DVEEE 240

Query: 241 EEGM---IPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVD 300
           EE M   +PSA EV+KME+FVRGLANLKGPTL+ KDIA+AALYLASDESKYISGHNLVVD
Sbjct: 241 EECMNYGVPSAAEVDKMEEFVRGLANLKGPTLKPKDIAQAALYLASDESKYISGHNLVVD 300

Query: 301 GGITTSRNCVGL 307
           GG+TTSRNC+GL
Sbjct: 301 GGVTTSRNCIGL 308

[Close]

BLAST of MC05g1586 vs. ExPASy TrEMBL
Match: A0A6J1I659 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111470263 PE=4 SV=1)

HSP 1 Score: 478 bits (1230), Expect = 2.99e-168
Identity = 261/309 (84.47%), Postives = 278/309 (89.97%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSP-PSHKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60
           MTAQV+PEQ    + IHLL RD   SP PS+KRLEGKVAIVTGGAKGIGEATVRLFAKHG
Sbjct: 1   MTAQVLPEQALQSN-IHLLQRD---SPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60

Query: 61  ARVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAG 120
           A+VVIADVEDLLG ALADTLSP  VTFVHCDVS EED+EN INST+ R+GQVD++FNNAG
Sbjct: 61  AKVVIADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAG 120

Query: 121 VLGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVL 180
           VLGNQS+ RKSIVDFDP+EFERVM VNVKGVALGIKHAARVMIPR +GCIISTASVAGV 
Sbjct: 121 VLGNQSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVS 180

Query: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEE 240
           GG+GPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR  DD DE  
Sbjct: 181 GGMGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR--DDGDECM 240

Query: 241 EEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGI 300
             G IPS  EV+KME+FVRGLANLKGPTLR KDIAEAALYLASDESKY+SGHNLVVDGGI
Sbjct: 241 NFG-IPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGI 300

Query: 301 TTSRNCVGL 307
           TTSRNC+GL
Sbjct: 301 TTSRNCIGL 302

[Close]

BLAST of MC05g1586 vs. ExPASy TrEMBL
Match: A0A061EQT3 (NAD(P)-binding Rossmann-fold superfamily protein OS=Theobroma cacao OX=3641 GN=TCM_021573 PE=4 SV=1)

HSP 1 Score: 478 bits (1229), Expect = 4.57e-168
Identity = 247/308 (80.19%), Postives = 276/308 (89.61%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           M AQVMPE+   L ++H+L R+ + S P  KRLEGK+AIVTGGA+GIGEATVRLFA+HGA
Sbjct: 1   MPAQVMPEKT--LQSLHVLGRE-NNSSPHPKRLEGKIAIVTGGARGIGEATVRLFARHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENAINSTIRRYGQVDVIFNNAGV 120
           +VVIAD+ED LGAALA++LSP  VTFVHCDV  EED+EN INSTI RYG++D++FNNAGV
Sbjct: 61  KVVIADLEDTLGAALANSLSP-SVTFVHCDVRLEEDIENLINSTISRYGRLDILFNNAGV 120

Query: 121 LGNQSQRKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGG 180
           LGNQ + KSIVDFD +EF+ VMRVNV+G+ALGIKHAAR MIPRG GCIISTASVAGV+GG
Sbjct: 121 LGNQKKHKSIVDFDVDEFDNVMRVNVRGMALGIKHAARAMIPRGGGCIISTASVAGVMGG 180

Query: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEEE 240
           LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDD+EE+  
Sbjct: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDEEEDSI 240

Query: 241 GM-IPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IP  QE EKME+FVRGLANLKGPTLRAKDIAEAALYLASDESKY+SGHNLVVDGG+T
Sbjct: 241 NFGIPCQQETEKMEEFVRGLANLKGPTLRAKDIAEAALYLASDESKYVSGHNLVVDGGVT 300

Query: 301 TSRNCVGL 307
           TSRNCVGL
Sbjct: 301 TSRNCVGL 304

[Close]

BLAST of MC05g1586 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 396.7 bits (1018), Expect = 1.6e-110
Identity = 208/312 (66.67%), Postives = 249/312 (79.81%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           M AQV+ E Q   H++H    +   +    KRLEGKVAI+TGGA GIG+ATV LFA+HGA
Sbjct: 1   MPAQVIAE-QTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQ----VTFVHCDVSSEEDVENAINSTIRRYGQVDVIFN 120
            VVIADV+++ G++LA +LS  +    V F+ CDVS E DVEN +N T+ RYG++D++FN
Sbjct: 61  TVVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFN 120

Query: 121 NAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRG-SGCIISTASVA 180
           NAGVLG+Q + KSI+DFD +EF+ VMRVNV+GV LG+KH AR MI RG  GCIISTASVA
Sbjct: 121 NAGVLGDQKKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVA 180

Query: 181 GVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDD 240
           GV+GG+GPHAYTASKHAIVGLTKN ACELG+YGIRVNCISPFGVATSMLVNAWR     D
Sbjct: 181 GVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGD 240

Query: 241 EEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVD 300
            E++        +VE+ME+FVR LANLKG TLRA DIAEAALYLASDESKY++GHNLVVD
Sbjct: 241 VEDD--------DVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVVD 300

Query: 301 GGITTSRNCVGL 308
           GG+TT+RNCVGL
Sbjct: 301 GGVTTARNCVGL 303

[Close]

BLAST of MC05g1586 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 7.7e-68
Identity = 134/269 (49.81%), Postives = 180/269 (66.91%), Query Frame = 0


Query: 31  KRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCD 90
           K+LEGKVA++TGGA G+G+AT   F +HGARVVIAD++   G   A  L   +  FV CD
Sbjct: 39  KKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELG-SEAEFVRCD 98

Query: 91  VSSEEDVENAINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVA 150
           V+ E D+  A+  T+ RYG++DV++NNAG++G  +   SI   D  EFERVMR+NV GV 
Sbjct: 99  VTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTP-ASISQLDMTEFERVMRINVFGVV 158

Query: 151 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 210
            GIKHAA+ MIP  SGCI+ T+SVAGV GGL PH+YT SK    G+ K+ A EL  +G+R
Sbjct: 159 SGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVR 218

Query: 211 VNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAK 270
           +NCISP  VAT + ++  +            + P   E EK+ + V+G+  LKG      
Sbjct: 219 INCISPGTVATPLTLSYLQK-----------VFPKVSE-EKLRETVKGMGELKGAECEEA 278

Query: 271 DIAEAALYLASDESKYISGHNLVVDGGIT 300
           D+A+AALYLAS++ KY++GHNLVVDGG+T
Sbjct: 279 DVAKAALYLASNDGKYVTGHNLVVDGGMT 293

[Close]

BLAST of MC05g1586 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.6e-65
Identity = 132/269 (49.07%), Postives = 184/269 (68.40%), Query Frame = 0


Query: 32  RLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCDV 91
           RL+GK+AI+TGGA GIG   VRLF  HGA+VVI D+++ LG  LA ++   + +F  C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 92  SSEEDVENAINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVAL 151
           + E DVENA+  T+ ++G++DV+F+NAGVL       S++D D E F+R M VNV+G A 
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVL---EAFGSVLDLDLEAFDRTMAVNVRGAAA 124

Query: 152 GIKHAARVMIPRGS-GCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 211
            IKHAAR M+  G+ G I+ T S+A  +GG GPH+YTASKHA++GL ++    LG+YGIR
Sbjct: 125 FIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIR 184

Query: 212 VNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAK 271
           VN ++P+GVAT M  +A+                + + V+ +E++   L NLKG  L+A+
Sbjct: 185 VNGVAPYGVATGM-TSAY----------------NEEAVKMLEEYGEALGNLKGVVLKAR 244

Query: 272 DIAEAALYLASDESKYISGHNLVVDGGIT 300
            IAEAAL+LASD+S YISG NLVVDGG +
Sbjct: 245 HIAEAALFLASDDSVYISGQNLVVDGGFS 253

[Close]

BLAST of MC05g1586 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 6.1e-65
Identity = 131/268 (48.88%), Postives = 183/268 (68.28%), Query Frame = 0


Query: 33  LEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCDVS 92
           L+GK+AI+TGGA GIG   VRLF  HGA+VVI D+++ LG  LA ++   + +F  C+V+
Sbjct: 44  LDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNVT 103

Query: 93  SEEDVENAINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVALG 152
            E DVENA+  T+ ++G++DV+F+NAGVL       S++D D E F+R M VNV+G A  
Sbjct: 104 DETDVENAVKFTVEKHGKLDVLFSNAGVL---EAFGSVLDLDLEAFDRTMAVNVRGAAAF 163

Query: 153 IKHAARVMIPRGS-GCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRV 212
           IKHAAR M+  G+ G I+ T S+A  +GG GPH+YTASKHA++GL ++    LG+YGIRV
Sbjct: 164 IKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRV 223

Query: 213 NCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKD 272
           N ++P+GVAT M  +A+                + + V+ +E++   L NLKG  L+A+ 
Sbjct: 224 NGVAPYGVATGM-TSAY----------------NEEAVKMLEEYGEALGNLKGVVLKARH 283

Query: 273 IAEAALYLASDESKYISGHNLVVDGGIT 300
           IAEAAL+LASD+S YISG NLVVDGG +
Sbjct: 284 IAEAALFLASDDSVYISGQNLVVDGGFS 291

[Close]

BLAST of MC05g1586 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 242.7 bits (618), Expect = 4.0e-64
Identity = 128/277 (46.21%), Postives = 181/277 (65.34%), Query Frame = 0


Query: 31  KRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTL----SPCQVTF 90
           +RL GKVA++TGGA GIGE+ VRLF KHGA+V I D++D LG  +  +L    S     F
Sbjct: 16  QRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFF 75

Query: 91  VHCDVSSEEDVENAINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNV 150
           +H DV  E+D+ NA++  ++ +G +D++ NNAG+ G  +    I ++   EFE    VNV
Sbjct: 76  IHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCG--APCPDIRNYSLSEFEMTFDVNV 135

Query: 151 KGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGR 210
           KG  L +KHAARVMIP   G I+S  SV GV+GG+GPH+Y  SKHA++GLT++ A ELG+
Sbjct: 136 KGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQ 195

Query: 211 YGIRVNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPT 270
           +GIRVNC+SP+ VAT + +    +H  ++E  E+  +          +F    ANLKG  
Sbjct: 196 HGIRVNCVSPYAVATKLAL----AHLPEEERTEDAFV-------GFRNFAAANANLKGVE 255

Query: 271 LRAKDIAEAALYLASDESKYISGHNLVVDGGITTSRN 304
           L   D+A A L+LASD+S+YISG NL++DGG T + +
Sbjct: 256 LTVDDVANAVLFLASDDSRYISGDNLMIDGGFTCTNH 279

The following BLAST results are available for this feature:

BLAST of MC05g1586 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCU0	2.3e-109	66.67	Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ...	[more]
P50160	5.2e-101	66.90	Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1	[more]
Q94KL7	2.1e-70	49.45	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
F1SWA0	1.4e-66	48.57	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]
Q7FAE1	1.9e-66	51.26	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]

BLAST of MC05g1586 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022934318.1	5.33e-172	84.42	short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 S...	[more]
XP_022983300.1	1.78e-170	83.77	short-chain dehydrogenase reductase 2a-like [Cucurbita maxima]	[more]
XP_023528551.1	2.04e-170	84.42	short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo]	[more]
XP_004137687.1	8.05e-170	82.37	short-chain dehydrogenase reductase 2a [Cucumis sativus] >KGN58711.1 hypothetica...	[more]
XP_038905282.1	1.51e-169	82.20	short-chain dehydrogenase reductase 2a [Benincasa hispida]	[more]

BLAST of MC05g1586 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F7B6	2.58e-172	84.42	short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1J5H2	8.60e-171	83.77	short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A0A0LF57	3.90e-170	82.37	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1	[more]
A0A6J1I659	2.99e-168	84.47	short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A061EQT3	4.57e-168	80.19	NAD(P)-binding Rossmann-fold superfamily protein OS=Theobroma cacao OX=3641 GN=T...	[more]

BLAST of MC05g1586 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51680.1	1.6e-110	66.67	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	7.7e-68	49.81	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.2	1.6e-65	49.07	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.1	6.1e-65	48.88	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G52340.1	4.0e-64	46.21	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 109..120 score: 50.13 coord: 37..54 score: 51.21 coord: 160..176 score: 35.38 coord: 186..205 score: 44.24 coord: 207..224 score: 41.2 coord: 262..282 score: 30.07
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 109..120 score: 48.28 coord: 186..205 score: 38.49 coord: 166..174 score: 45.22
None	No IPR available	GENE3D	3.40.50.720		coord: 23..305 e-value: 3.8E-82 score: 277.4
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 42..249 e-value: 1.5E-51 score: 175.3
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 32..218 e-value: 1.9E-9 score: 34.9
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 39..239 e-value: 2.4E-19 score: 67.4
None	No IPR available	PANTHER	PTHR43180:SF58	SHORT-CHAIN DEHYDROGENASE REDUCTASE 2A-LIKE	coord: 1..307
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 1..307
None	No IPR available	CDD	cd05326	secoisolariciresinol-DH_like_SDR_c	coord: 32..299 e-value: 5.28601E-129 score: 365.625
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 34..299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g1586.1	MC05g1586.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004316	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
molecular_function	GO:0016491	oxidoreductase activity