MC05g1538 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g1538
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	lactosylceramide 4-alpha-galactosyltransferase
Location	MC05: 19166297 .. 19167538 (-)
RNA-Seq Expression	MC05g1538
Synteny	MC05g1538

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGGAAGGTGTGCTATATCACCAATATTGCCAACTGTCTTGTTTGCGGCCATCATCTTCCTTGTATGTGGAGATGGCATCATCTACAAAGTTTCGATTCGAACAGTATCGAGAGATGAGAACAAAATTTCGAACTCAACAATTACGTGCGTAACACAAGTACAAATTGAGTCAACTTTAGAAGAAGAGCCACTGCAGTCCTCTACAGAATACAAAGATGATGACAATAGTGAAACTCTCGATTCCTTGGTAGTTCCATCCGATCTTTCAGAAAAAAAAGCAAGAATTGATTGGTTTAGGAAACACCTTCCCGACTTTCGAATTTTGCAGTCAAACAACTTGACGCAGCAGTTTGATAATCGACTTCTGGAGTTTCTTAGCCACGAGTGCGAGACGCAATTCTTCATGACATGGATATCTCCAGCAAGGTCTTTCAGAGGAAGAGAATTACTGGCAGCAGAAAGTGTTTTTAAGTCTCACCCCCGTGGATGCTTAACTATTCTATCAAGAACGCTGGATTCAGAAAGAGGGTACAAGATTCTTAAACCTCTACTTGATCGTGCGTTTAAAATTCTGGCAATCACACCGGACTTGCCTTCTCTATTCAAAAACACCCCTGTTGAGGCTTGGTTTGATGAGATGAAGAGAGGAAAAAAGGACCCAGGTCTGATTCCATTAGCTCAAAATCTATCCAACTTGATGAGACTTGCAGTTTTATACAGGTATGGAGGTGTTTACATAGATACAGATTTTATTGTTTTGAAGTCTTTCATGGGGTTGAAGAACTCTATTGGCGCACAAAGTATTGATCCAATATCTAAGAGCTGGACAATACTAAACAACGCTGTTCTTGTTTTTGATAAGAACCATCCTCTTCTAGAGAAATTCATGGAAAATTTTGCATCAAATTTTGATGGGAGCAGATGGGGATATAATGGACCTTTTCTGGTCTCCAGAGTAGTAGCAAAGGTAACAGGAGCCAAAGCTACACCTGGTTTCAATGTCACGGTATTACCACCAGTGGCGTTCTACCCAGTGGACTGGAATAAGATTGGTGAACTTTTTAAGAAGCCAGAAAACCAGGCTACTTCTAGTTGGGCCAAAGCAGTGCTTGCTCAGCTAAATAACGAGGCTTATGGAATACACCTATGGAACAAGCATAGTAAGAGTTATATCATGCAAAAAGGAAGCGTCATAGAAAGATTAGTCTCTGATCATTGCATTATTTGCAGCAACACT

mRNA sequence

ATGTCTGGAAGGTGTGCTATATCACCAATATTGCCAACTGTCTTGTTTGCGGCCATCATCTTCCTTGTATGTGGAGATGGCATCATCTACAAAGTTTCGATTCGAACAGTATCGAGAGATGAGAACAAAATTTCGAACTCAACAATTACGTGCGTAACACAAGTACAAATTGAGTCAACTTTAGAAGAAGAGCCACTGCAGTCCTCTACAGAATACAAAGATGATGACAATAGTGAAACTCTCGATTCCTTGGTAGTTCCATCCGATCTTTCAGAAAAAAAAGCAAGAATTGATTGGTTTAGGAAACACCTTCCCGACTTTCGAATTTTGCAGTCAAACAACTTGACGCAGCAGTTTGATAATCGACTTCTGGAGTTTCTTAGCCACGAGTGCGAGACGCAATTCTTCATGACATGGATATCTCCAGCAAGAACGCTGGATTCAGAAAGAGGGTACAAGATTCTTAAACCTCTACTTGATCGTGCGTTTAAAATTCTGGCAATCACACCGGACTTGCCTTCTCTATTCAAAAACACCCCTGTTGAGGCTTGGTTTGATGAGATGAAGAGAGGAAAAAAGGACCCAGGTCTGATTCCATTAGCTCAAAATCTATCCAACTTGATGAGACTTGCAGTTTTATACAGGTATGGAGGTGTTTACATAGATACAGATTTTATTGTTTTGAAGTCTTTCATGGGGTTGAAGAACTCTATTGGCGCACAAAGTATTGATCCAATATCTAAGAGCTGGACAATACTAAACAACGCTGTTCTTGTTTTTGATAAGAACCATCCTCTTCTAGAGAAATTCATGGAAAATTTTGCATCAAATTTTGATGGGAGCAGATGGGGATATAATGGACCTTTTCTGGTCTCCAGAGTAGTAGCAAAGGTAACAGGAGCCAAAGCTACACCTGGTTTCAATGTCACGGTATTACCACCAGTGGCGTTCTACCCAGTGGACTGGAATAAGATTGGTGAACTTTTTAAGAAGCCAGAAAACCAGGCTACTTCTAGTTGGGCCAAAGCAGTGCTTGCTCAGCTAAATAACGAGGCTTATGGAATACACCTATGGAACAAGCATAGTAAGAGTTATATCATGCAAAAAGGAAGCGTCATAGAAAGATTAGTCTCTGATCATTGCATTATTTGCAGCAACACT

Coding sequence (CDS)

ATGTCTGGAAGGTGTGCTATATCACCAATATTGCCAACTGTCTTGTTTGCGGCCATCATCTTCCTTGTATGTGGAGATGGCATCATCTACAAAGTTTCGATTCGAACAGTATCGAGAGATGAGAACAAAATTTCGAACTCAACAATTACGTGCGTAACACAAGTACAAATTGAGTCAACTTTAGAAGAAGAGCCACTGCAGTCCTCTACAGAATACAAAGATGATGACAATAGTGAAACTCTCGATTCCTTGGTAGTTCCATCCGATCTTTCAGAAAAAAAAGCAAGAATTGATTGGTTTAGGAAACACCTTCCCGACTTTCGAATTTTGCAGTCAAACAACTTGACGCAGCAGTTTGATAATCGACTTCTGGAGTTTCTTAGCCACGAGTGCGAGACGCAATTCTTCATGACATGGATATCTCCAGCAAGAACGCTGGATTCAGAAAGAGGGTACAAGATTCTTAAACCTCTACTTGATCGTGCGTTTAAAATTCTGGCAATCACACCGGACTTGCCTTCTCTATTCAAAAACACCCCTGTTGAGGCTTGGTTTGATGAGATGAAGAGAGGAAAAAAGGACCCAGGTCTGATTCCATTAGCTCAAAATCTATCCAACTTGATGAGACTTGCAGTTTTATACAGGTATGGAGGTGTTTACATAGATACAGATTTTATTGTTTTGAAGTCTTTCATGGGGTTGAAGAACTCTATTGGCGCACAAAGTATTGATCCAATATCTAAGAGCTGGACAATACTAAACAACGCTGTTCTTGTTTTTGATAAGAACCATCCTCTTCTAGAGAAATTCATGGAAAATTTTGCATCAAATTTTGATGGGAGCAGATGGGGATATAATGGACCTTTTCTGGTCTCCAGAGTAGTAGCAAAGGTAACAGGAGCCAAAGCTACACCTGGTTTCAATGTCACGGTATTACCACCAGTGGCGTTCTACCCAGTGGACTGGAATAAGATTGGTGAACTTTTTAAGAAGCCAGAAAACCAGGCTACTTCTAGTTGGGCCAAAGCAGTGCTTGCTCAGCTAAATAACGAGGCTTATGGAATACACCTATGGAACAAGCATAGTAAGAGTTATATCATGCAAAAAGGAAGCGTCATAGAAAGATTAGTCTCTGATCATTGCATTATTTGCAGCAACACT

Protein sequence

MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIESTLEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFDNRLLEFLSHECETQFFMTWISPARTLDSERGYKILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVLYRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMENFASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPENQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT

Homology

[Close]

BLAST of MC05g1538 vs. ExPASy Swiss-Prot
Match: Q67BJ4 (Lactosylceramide 4-alpha-galactosyltransferase OS=Mus musculus OX=10090 GN=A4galt PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.1e-30
Identity = 78/219 (35.62%), Postives = 123/219 (56.16%), Query Frame = 0


Query: 171 DLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVLYRYGGVYIDTDFIVLKS 230
           DL  LF++TP+ AW+ E  R + +P  +P+   LS+  R+A+L+++GG+Y+DTDFIVLK+
Sbjct: 151 DLQELFEDTPLAAWYSE-ARHRWEPYQLPV---LSDASRIALLWKFGGIYLDTDFIVLKN 210

Query: 231 FMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMENFASNFDGSRWGYNGPFL 290
            + L N++G Q       S  +LN A L F++ H  L   + +F +N++G  WG+ GP L
Sbjct: 211 LLNLTNTLGIQ-------SRYVLNGAFLAFERKHEFLALCLHDFVANYNGWIWGHQGPQL 270

Query: 291 VSRVVAKVTG----AKATPGFNVTVLPPVAFYPVDWNKIGELFK--KPENQATSSWAKAV 350
           ++RV  K        K+     VT LPP AFYP+ W    + F+   PE           
Sbjct: 271 LTRVFKKWCSIQSLEKSHACRGVTALPPEAFYPIPWQNWKKYFEDISPEE---------- 330

Query: 351 LAQLNNEAYGIHLWNKHSKSYIMQKGS--VIERLVSDHC 382
           L QL N  Y +H+WNK S+   ++  S  ++ +L + +C
Sbjct: 331 LTQLLNATYAVHVWNKKSQGTHLEATSKALLAQLHARYC 348

[Close]

BLAST of MC05g1538 vs. ExPASy Swiss-Prot
Match: Q9JI93 (Lactosylceramide 4-alpha-galactosyltransferase OS=Rattus norvegicus OX=10116 GN=A4galt PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.3e-29
Identity = 79/221 (35.75%), Postives = 126/221 (57.01%), Query Frame = 0


Query: 171 DLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVLYRYGGVYIDTDFIVLKS 230
           DL  LF++TP+ AW+ E +  + +P L+P+   LS+  R+A+L+++GG+Y+DTDFIVLK+
Sbjct: 152 DLQELFEDTPLAAWYLEAQH-RWEPYLLPV---LSDASRIALLWKFGGIYLDTDFIVLKN 211

Query: 231 FMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMENFASNFDGSRWGYNGPFL 290
              L N +G Q       S  +LN A L F++ H  L   + +F ++++G  WG+ GP L
Sbjct: 212 LRNLTNMLGIQ-------SRYVLNGAFLAFERKHEFLALCIRDFVAHYNGWIWGHQGPQL 271

Query: 291 VSRVVAK------VTGAKATPGFNVTVLPPVAFYPVDWNKIGELFK--KPENQATSSWAK 350
           ++RV  K      +  ++A  G  VT LPP AFYP+ W    + F+   PE         
Sbjct: 272 LTRVFKKWCSIHSLKESRACRG--VTALPPEAFYPIPWQNWKKYFEDVSPEE-------- 331

Query: 351 AVLAQLNNEAYGIHLWNKHSKSYIMQKGS--VIERLVSDHC 382
             LAQL N  Y +H+WNK S+   ++  S  ++ +L + +C
Sbjct: 332 --LAQLLNATYAVHVWNKKSQGTHLEATSRALLAQLHARYC 349

[Close]

BLAST of MC05g1538 vs. ExPASy Swiss-Prot
Match: Q9N289 (Lactosylceramide 4-alpha-galactosyltransferase (Fragment) OS=Pongo pygmaeus OX=9600 GN=A4GALT PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 7.4e-28
Identity = 71/199 (35.68%), Postives = 111/199 (55.78%), Query Frame = 0


Query: 171 DLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVLYRYGGVYIDTDFIVLKS 230
           DL  LF++TP+  W+  + +G+ +P L+P+   LS+  R+A+++++GG+Y+DTDFIVLK+
Sbjct: 10  DLRELFRDTPLADWYTAV-QGRWEPYLLPV---LSDASRIALMWKFGGIYLDTDFIVLKN 69

Query: 231 FMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMENFASNFDGSRWGYNGPFL 290
              L N +G Q       S  +LN A L F + H  +   M +F  +++G  WG+ GP L
Sbjct: 70  LRNLTNVLGTQ-------SRYVLNGAFLAFQRRHEFMALCMRDFVDHYNGWIWGHQGPQL 129

Query: 291 VSRVVAKVTG----AKATPGFNVTVLPPVAFYPVDWNKIGELFK--KPENQATSSWAKAV 350
           ++RV  K       A++     VT LPP AFYP+ W    + F+   PE           
Sbjct: 130 LTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDISPEE---------- 187

Query: 351 LAQLNNEAYGIHLWNKHSK 364
           L +L N  Y +H+WNK S+
Sbjct: 190 LPRLLNATYAVHVWNKKSQ 187

[Close]

BLAST of MC05g1538 vs. ExPASy Swiss-Prot
Match: Q9NPC4 (Lactosylceramide 4-alpha-galactosyltransferase OS=Homo sapiens OX=9606 GN=A4GALT PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 2.1e-27
Identity = 70/199 (35.18%), Postives = 112/199 (56.28%), Query Frame = 0


Query: 171 DLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVLYRYGGVYIDTDFIVLKS 230
           DL  LF++TP+  W+  + +G+ +P L+P+   LS+  R+A+++++GG+Y+DTDFIVLK+
Sbjct: 145 DLRELFRDTPLADWYAAV-QGRWEPYLLPV---LSDASRIALMWKFGGIYLDTDFIVLKN 204

Query: 231 FMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMENFASNFDGSRWGYNGPFL 290
              L N +G Q       S  +LN A L F++ H  +   M +F  +++G  WG+ GP L
Sbjct: 205 LRNLTNVLGTQ-------SRYVLNGAFLAFERRHEFMALCMRDFVDHYNGWIWGHQGPQL 264

Query: 291 VSRVVAKVTG----AKATPGFNVTVLPPVAFYPVDWNKIGELFK--KPENQATSSWAKAV 350
           ++RV  K       A++     VT LPP AFYP+ W    + F+   PE           
Sbjct: 265 LTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEE---------- 322

Query: 351 LAQLNNEAYGIHLWNKHSK 364
           L +L +  Y +H+WNK S+
Sbjct: 325 LPRLLSATYAVHVWNKKSQ 322

[Close]

BLAST of MC05g1538 vs. ExPASy Swiss-Prot
Match: Q9N291 (Lactosylceramide 4-alpha-galactosyltransferase OS=Pan troglodytes OX=9598 GN=A4GALT PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.8e-27
Identity = 70/199 (35.18%), Postives = 112/199 (56.28%), Query Frame = 0


Query: 171 DLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVLYRYGGVYIDTDFIVLKS 230
           DL  LF++TP+  W+  + +G+ +P L+P+   LS+  R+A+++++GG+Y+DTDFIVLK+
Sbjct: 145 DLRELFQDTPLADWYAAV-QGRWEPYLLPV---LSDASRIALMWKFGGIYLDTDFIVLKN 204

Query: 231 FMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMENFASNFDGSRWGYNGPFL 290
              L N +G Q       S  +LN A L F++ H  +   M +F  +++G  WG+ GP L
Sbjct: 205 LRNLTNVLGTQ-------SRYVLNGAFLAFERRHEFMALCMRDFVDHYNGWIWGHQGPQL 264

Query: 291 VSRVVAKVTG----AKATPGFNVTVLPPVAFYPVDWNKIGELFK--KPENQATSSWAKAV 350
           ++RV  K       A++     VT LPP AFYP+ W    + F+   PE           
Sbjct: 265 LTRVFKKWCSIRSLAESRSCRGVTTLPPEAFYPIPWQDWKKYFEDINPEE---------- 322

Query: 351 LAQLNNEAYGIHLWNKHSK 364
           L +L +  Y +H+WNK S+
Sbjct: 325 LPRLLSATYAVHVWNKKSQ 322

[Close]

BLAST of MC05g1538 vs. NCBI nr
Match: XP_022145489.1 (lactosylceramide 4-alpha-galactosyltransferase [Momordica charantia])

HSP 1 Score: 762 bits (1967), Expect = 5.01e-277
Identity = 386/414 (93.24%), Postives = 387/414 (93.48%), Query Frame = 0


Query: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60
           MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST
Sbjct: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60

Query: 61  LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD 120
           LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD
Sbjct: 61  LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD 120

Query: 121 NRLLEFLSHECETQFFMTWISPAR---------------------------TLDSERGYK 180
           NRLLEFLSHECETQFFMTWISPAR                           TLDSERGYK
Sbjct: 121 NRLLEFLSHECETQFFMTWISPARSFRGRELLAAESVFKSHPRGCLTILSRTLDSERGYK 180

Query: 181 ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL 240
           ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL
Sbjct: 181 ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL 240

Query: 241 YRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN 300
           Y+YGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN
Sbjct: 241 YKYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN 300

Query: 301 FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE 360
           FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE
Sbjct: 301 FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE 360

Query: 361 NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 387
           NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT
Sbjct: 361 NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 414

[Close]

BLAST of MC05g1538 vs. NCBI nr
Match: XP_016899682.1 (PREDICTED: lactosylceramide 4-alpha-galactosyltransferase [Cucumis melo] >KAA0044161.1 lactosylceramide 4-alpha-galactosyltransferase [Cucumis melo var. makuwa] >TYK24973.1 lactosylceramide 4-alpha-galactosyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 652 bits (1682), Expect = 1.31e-233
Identity = 326/414 (78.74%), Postives = 352/414 (85.02%), Query Frame = 0


Query: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60
           MS RCAISPILPT+L AAIIFL+CGDGIIYKVSIRTVS DE + SNSTITC+TQ+QI+ T
Sbjct: 1   MSERCAISPILPTILLAAIIFLICGDGIIYKVSIRTVSLDEKRNSNSTITCITQLQIKQT 60

Query: 61  LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD 120
           L  EP QSS E +D+D+SET DSLVVPS+LS K AR+DWFR HLP+FRILQSNNLTQQF 
Sbjct: 61  LVGEPRQSSPENRDEDDSETFDSLVVPSNLSAKDARVDWFRNHLPNFRILQSNNLTQQFH 120

Query: 121 NRLLEFLSHECETQFFMTWISPAR---------------------------TLDSERGYK 180
           NRLLEFLSHECE QFFMTW+SPAR                           TLDSERG K
Sbjct: 121 NRLLEFLSHECEVQFFMTWVSPARSFRERELMAAESVFKSHPRGCLTIISRTLDSERGCK 180

Query: 181 ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL 240
           ILKPLLD  FKI AI PDLP LF+NTPVEAWFDEMK GKKDPG IPLAQNLSNLMRLAVL
Sbjct: 181 ILKPLLDHGFKIQAIAPDLPLLFRNTPVEAWFDEMKSGKKDPGQIPLAQNLSNLMRLAVL 240

Query: 241 YRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN 300
           Y+YGGVYIDTDFIVLKSFMGLKNSIGAQSIDP++K+WTILNNAVLVFDK HPLLEKFMEN
Sbjct: 241 YKYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPVTKNWTILNNAVLVFDKKHPLLEKFMEN 300

Query: 301 FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE 360
           FASNFDGSRWGYNGPFLV+RV+AKVTGA+A+PGFNVTVLPP AFYPVDW KIGELFKKPE
Sbjct: 301 FASNFDGSRWGYNGPFLVTRVIAKVTGARASPGFNVTVLPPAAFYPVDWIKIGELFKKPE 360

Query: 361 NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 387
           N+A  SWAKA LAQL+NE YGIHLWNK SKSY++QKGSVIERL SDHCIICS T
Sbjct: 361 NRAVESWAKARLAQLDNETYGIHLWNKQSKSYVIQKGSVIERLFSDHCIICSYT 414

[Close]

BLAST of MC05g1538 vs. NCBI nr
Match: XP_004137912.1 (lactosylceramide 4-alpha-galactosyltransferase [Cucumis sativus] >KGN58777.1 hypothetical protein Csa_001279 [Cucumis sativus])

HSP 1 Score: 641 bits (1653), Expect = 3.42e-229
Identity = 320/414 (77.29%), Postives = 348/414 (84.06%), Query Frame = 0


Query: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60
           MS RCAISPILPT+L AAIIFL+CGD IIYKVSIRTVS DE + SNST TC+TQ+QI+ T
Sbjct: 1   MSERCAISPILPTILLAAIIFLICGDSIIYKVSIRTVSIDEKRNSNSTSTCITQLQIKQT 60

Query: 61  LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD 120
           L  EP QSS E +D+D+SET DSLVVPS+LS K+AR+DWFR HLP+FRILQSNNLTQQF 
Sbjct: 61  LVGEPRQSSPENRDEDDSETFDSLVVPSNLSAKEARVDWFRNHLPNFRILQSNNLTQQFH 120

Query: 121 NRLLEFLSHECETQFFMTWISPAR---------------------------TLDSERGYK 180
           +RLLEFLSHECE QFFMTW+SPAR                           TLDSERG K
Sbjct: 121 DRLLEFLSHECEVQFFMTWVSPARSFRERELMAAESVFKSHPRGCLTIISRTLDSERGCK 180

Query: 181 ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL 240
           ILKPLLD  FKI AI PDLP LFKNTPVEAWFDEMK GKKDPG IPLAQNLSNLMRLAVL
Sbjct: 181 ILKPLLDHGFKIQAIAPDLPLLFKNTPVEAWFDEMKSGKKDPGQIPLAQNLSNLMRLAVL 240

Query: 241 YRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN 300
           Y+YGGVYIDTDFIVLKSFMGLKNSIGAQSIDP++K+WTILNNAVLVFDK HPLLEKFMEN
Sbjct: 241 YKYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPVTKNWTILNNAVLVFDKKHPLLEKFMEN 300

Query: 301 FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE 360
           FASNFDGSRWG+NGPFLVSRV+AK+TGA+A PGFNVT+LPP AFYPVDW KIGELFKKP 
Sbjct: 301 FASNFDGSRWGHNGPFLVSRVIAKITGARAKPGFNVTILPPAAFYPVDWIKIGELFKKPG 360

Query: 361 NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 387
           N+A  SWAKA L QLNNE YGIHLWNK SKSY++QKGSVIERL +DHCIICS T
Sbjct: 361 NRAVESWAKAKLDQLNNETYGIHLWNKQSKSYVIQKGSVIERLFADHCIICSYT 414

[Close]

BLAST of MC05g1538 vs. NCBI nr
Match: XP_022948708.1 (lactosylceramide 4-alpha-galactosyltransferase-like [Cucurbita moschata])

HSP 1 Score: 629 bits (1622), Expect = 1.34e-224
Identity = 319/415 (76.87%), Postives = 344/415 (82.89%), Query Frame = 0


Query: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60
           MS RC +SPILPT+L AAII  +CGDGIIYK SIRTVS +ENK SNSTITCVTQ      
Sbjct: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQ------ 60

Query: 61  LEEEPLQSSTEYKD-DDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQF 120
              EPL+SS E ++ DD+S T DSL+VP +LS KKARIDWFRKHLPDFRILQSNN TQQF
Sbjct: 61  ---EPLKSSPENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQF 120

Query: 121 DNRLLEFLSHECETQFFMTWISP---------------------------ARTLDSERGY 180
            NRL EFLSH+CETQFFMTWISP                           +RTLDSERGY
Sbjct: 121 HNRLQEFLSHDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGY 180

Query: 181 KILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAV 240
           KIL+PLLD  FKILAITPDLPSLFKNTPVE WFDEMKRGKKDPG IPLAQNLSNLMRLAV
Sbjct: 181 KILQPLLDHGFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAV 240

Query: 241 LYRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFME 300
           LY+YGGVYIDTDFIVLKSFMGLKN IGAQSIDP+SK+WT LNNAVLVFDK HPLLEKFME
Sbjct: 241 LYKYGGVYIDTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFME 300

Query: 301 NFASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKP 360
           NFASNFDGSRWGYNGPFLVSRV+AKVTGA A PGFNVT+LPP AFYPVDW KIGELFKKP
Sbjct: 301 NFASNFDGSRWGYNGPFLVSRVIAKVTGAGARPGFNVTILPPAAFYPVDWTKIGELFKKP 360

Query: 361 ENQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 387
            N+A+++WAKA+LAQLN+EAYGIHLWNK SKSY +QKGSVIERLVS HCIIC  T
Sbjct: 361 GNRASANWAKAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 406

[Close]

BLAST of MC05g1538 vs. NCBI nr
Match: XP_022971397.1 (lactosylceramide 4-alpha-galactosyltransferase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 627 bits (1616), Expect = 1.09e-223
Identity = 314/415 (75.66%), Postives = 345/415 (83.13%), Query Frame = 0


Query: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60
           MS RC +SPILPT+L AAII  +CGDG+IYK SIRTVS +ENK SNSTITC+TQ      
Sbjct: 1   MSERCTVSPILPTILLAAIIIFICGDGMIYKASIRTVSINENKNSNSTITCITQ------ 60

Query: 61  LEEEPLQSSTEYKD-DDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQF 120
              EPL+SS E ++ DD+S T DSL+VP +LS KKARIDWFRKHLPDFRILQSNN TQQF
Sbjct: 61  ---EPLKSSPENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQF 120

Query: 121 DNRLLEFLSHECETQFFMTWISP---------------------------ARTLDSERGY 180
            NRL EFLSH+CETQFFMTWISP                           +RTLDSERGY
Sbjct: 121 HNRLQEFLSHDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGY 180

Query: 181 KILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAV 240
           KIL+PLLDR FKILAITPDLPSLFKNTPVE WFDEMKRGKKDPG IPLAQNLSNLMRLAV
Sbjct: 181 KILQPLLDRGFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAV 240

Query: 241 LYRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFME 300
           LY+YGGVYIDTDFIVLKSFMGLKN IGAQSIDP+SK+WT LNNAVLVFDK HPLLEKFME
Sbjct: 241 LYKYGGVYIDTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFME 300

Query: 301 NFASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKP 360
           NFASNFDGSRWGYNGPFLVSRV+AKVTGA   PGFNVT+LPP AFYPVDW KIGELFKKP
Sbjct: 301 NFASNFDGSRWGYNGPFLVSRVIAKVTGAGDRPGFNVTILPPAAFYPVDWTKIGELFKKP 360

Query: 361 ENQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 387
            N+A+++WAKA+L+QLN++AYGIHLWNK SKSY +QKGSVIE+LVSDHCI+C  T
Sbjct: 361 GNRASANWAKAMLSQLNDKAYGIHLWNKQSKSYTIQKGSVIEKLVSDHCIVCKYT 406

[Close]

BLAST of MC05g1538 vs. ExPASy TrEMBL
Match: A0A6J1CVE1 (lactosylceramide 4-alpha-galactosyltransferase OS=Momordica charantia OX=3673 GN=LOC111014925 PE=4 SV=1)

HSP 1 Score: 762 bits (1967), Expect = 2.43e-277
Identity = 386/414 (93.24%), Postives = 387/414 (93.48%), Query Frame = 0


Query: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60
           MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST
Sbjct: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60

Query: 61  LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD 120
           LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD
Sbjct: 61  LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD 120

Query: 121 NRLLEFLSHECETQFFMTWISPAR---------------------------TLDSERGYK 180
           NRLLEFLSHECETQFFMTWISPAR                           TLDSERGYK
Sbjct: 121 NRLLEFLSHECETQFFMTWISPARSFRGRELLAAESVFKSHPRGCLTILSRTLDSERGYK 180

Query: 181 ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL 240
           ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL
Sbjct: 181 ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL 240

Query: 241 YRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN 300
           Y+YGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN
Sbjct: 241 YKYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN 300

Query: 301 FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE 360
           FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE
Sbjct: 301 FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE 360

Query: 361 NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 387
           NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT
Sbjct: 361 NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 414

[Close]

BLAST of MC05g1538 vs. ExPASy TrEMBL
Match: A0A5A7TRE8 (Lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00900 PE=4 SV=1)

HSP 1 Score: 652 bits (1682), Expect = 6.35e-234
Identity = 326/414 (78.74%), Postives = 352/414 (85.02%), Query Frame = 0


Query: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60
           MS RCAISPILPT+L AAIIFL+CGDGIIYKVSIRTVS DE + SNSTITC+TQ+QI+ T
Sbjct: 1   MSERCAISPILPTILLAAIIFLICGDGIIYKVSIRTVSLDEKRNSNSTITCITQLQIKQT 60

Query: 61  LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD 120
           L  EP QSS E +D+D+SET DSLVVPS+LS K AR+DWFR HLP+FRILQSNNLTQQF 
Sbjct: 61  LVGEPRQSSPENRDEDDSETFDSLVVPSNLSAKDARVDWFRNHLPNFRILQSNNLTQQFH 120

Query: 121 NRLLEFLSHECETQFFMTWISPAR---------------------------TLDSERGYK 180
           NRLLEFLSHECE QFFMTW+SPAR                           TLDSERG K
Sbjct: 121 NRLLEFLSHECEVQFFMTWVSPARSFRERELMAAESVFKSHPRGCLTIISRTLDSERGCK 180

Query: 181 ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL 240
           ILKPLLD  FKI AI PDLP LF+NTPVEAWFDEMK GKKDPG IPLAQNLSNLMRLAVL
Sbjct: 181 ILKPLLDHGFKIQAIAPDLPLLFRNTPVEAWFDEMKSGKKDPGQIPLAQNLSNLMRLAVL 240

Query: 241 YRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN 300
           Y+YGGVYIDTDFIVLKSFMGLKNSIGAQSIDP++K+WTILNNAVLVFDK HPLLEKFMEN
Sbjct: 241 YKYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPVTKNWTILNNAVLVFDKKHPLLEKFMEN 300

Query: 301 FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE 360
           FASNFDGSRWGYNGPFLV+RV+AKVTGA+A+PGFNVTVLPP AFYPVDW KIGELFKKPE
Sbjct: 301 FASNFDGSRWGYNGPFLVTRVIAKVTGARASPGFNVTVLPPAAFYPVDWIKIGELFKKPE 360

Query: 361 NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 387
           N+A  SWAKA LAQL+NE YGIHLWNK SKSY++QKGSVIERL SDHCIICS T
Sbjct: 361 NRAVESWAKARLAQLDNETYGIHLWNKQSKSYVIQKGSVIERLFSDHCIICSYT 414

[Close]

BLAST of MC05g1538 vs. ExPASy TrEMBL
Match: A0A1S4DUL6 (lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo OX=3656 GN=LOC103486309 PE=4 SV=1)

HSP 1 Score: 652 bits (1682), Expect = 6.35e-234
Identity = 326/414 (78.74%), Postives = 352/414 (85.02%), Query Frame = 0


Query: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60
           MS RCAISPILPT+L AAIIFL+CGDGIIYKVSIRTVS DE + SNSTITC+TQ+QI+ T
Sbjct: 1   MSERCAISPILPTILLAAIIFLICGDGIIYKVSIRTVSLDEKRNSNSTITCITQLQIKQT 60

Query: 61  LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD 120
           L  EP QSS E +D+D+SET DSLVVPS+LS K AR+DWFR HLP+FRILQSNNLTQQF 
Sbjct: 61  LVGEPRQSSPENRDEDDSETFDSLVVPSNLSAKDARVDWFRNHLPNFRILQSNNLTQQFH 120

Query: 121 NRLLEFLSHECETQFFMTWISPAR---------------------------TLDSERGYK 180
           NRLLEFLSHECE QFFMTW+SPAR                           TLDSERG K
Sbjct: 121 NRLLEFLSHECEVQFFMTWVSPARSFRERELMAAESVFKSHPRGCLTIISRTLDSERGCK 180

Query: 181 ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL 240
           ILKPLLD  FKI AI PDLP LF+NTPVEAWFDEMK GKKDPG IPLAQNLSNLMRLAVL
Sbjct: 181 ILKPLLDHGFKIQAIAPDLPLLFRNTPVEAWFDEMKSGKKDPGQIPLAQNLSNLMRLAVL 240

Query: 241 YRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN 300
           Y+YGGVYIDTDFIVLKSFMGLKNSIGAQSIDP++K+WTILNNAVLVFDK HPLLEKFMEN
Sbjct: 241 YKYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPVTKNWTILNNAVLVFDKKHPLLEKFMEN 300

Query: 301 FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE 360
           FASNFDGSRWGYNGPFLV+RV+AKVTGA+A+PGFNVTVLPP AFYPVDW KIGELFKKPE
Sbjct: 301 FASNFDGSRWGYNGPFLVTRVIAKVTGARASPGFNVTVLPPAAFYPVDWIKIGELFKKPE 360

Query: 361 NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 387
           N+A  SWAKA LAQL+NE YGIHLWNK SKSY++QKGSVIERL SDHCIICS T
Sbjct: 361 NRAVESWAKARLAQLDNETYGIHLWNKQSKSYVIQKGSVIERLFSDHCIICSYT 414

[Close]

BLAST of MC05g1538 vs. ExPASy TrEMBL
Match: A0A0A0LDG3 (Gb3_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731870 PE=4 SV=1)

HSP 1 Score: 641 bits (1653), Expect = 1.65e-229
Identity = 320/414 (77.29%), Postives = 348/414 (84.06%), Query Frame = 0


Query: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60
           MS RCAISPILPT+L AAIIFL+CGD IIYKVSIRTVS DE + SNST TC+TQ+QI+ T
Sbjct: 1   MSERCAISPILPTILLAAIIFLICGDSIIYKVSIRTVSIDEKRNSNSTSTCITQLQIKQT 60

Query: 61  LEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD 120
           L  EP QSS E +D+D+SET DSLVVPS+LS K+AR+DWFR HLP+FRILQSNNLTQQF 
Sbjct: 61  LVGEPRQSSPENRDEDDSETFDSLVVPSNLSAKEARVDWFRNHLPNFRILQSNNLTQQFH 120

Query: 121 NRLLEFLSHECETQFFMTWISPAR---------------------------TLDSERGYK 180
           +RLLEFLSHECE QFFMTW+SPAR                           TLDSERG K
Sbjct: 121 DRLLEFLSHECEVQFFMTWVSPARSFRERELMAAESVFKSHPRGCLTIISRTLDSERGCK 180

Query: 181 ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL 240
           ILKPLLD  FKI AI PDLP LFKNTPVEAWFDEMK GKKDPG IPLAQNLSNLMRLAVL
Sbjct: 181 ILKPLLDHGFKIQAIAPDLPLLFKNTPVEAWFDEMKSGKKDPGQIPLAQNLSNLMRLAVL 240

Query: 241 YRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN 300
           Y+YGGVYIDTDFIVLKSFMGLKNSIGAQSIDP++K+WTILNNAVLVFDK HPLLEKFMEN
Sbjct: 241 YKYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPVTKNWTILNNAVLVFDKKHPLLEKFMEN 300

Query: 301 FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPE 360
           FASNFDGSRWG+NGPFLVSRV+AK+TGA+A PGFNVT+LPP AFYPVDW KIGELFKKP 
Sbjct: 301 FASNFDGSRWGHNGPFLVSRVIAKITGARAKPGFNVTILPPAAFYPVDWIKIGELFKKPG 360

Query: 361 NQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 387
           N+A  SWAKA L QLNNE YGIHLWNK SKSY++QKGSVIERL +DHCIICS T
Sbjct: 361 NRAVESWAKAKLDQLNNETYGIHLWNKQSKSYVIQKGSVIERLFADHCIICSYT 414

[Close]

BLAST of MC05g1538 vs. ExPASy TrEMBL
Match: A0A6J1GA34 (lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111452297 PE=4 SV=1)

HSP 1 Score: 629 bits (1622), Expect = 6.47e-225
Identity = 319/415 (76.87%), Postives = 344/415 (82.89%), Query Frame = 0


Query: 1   MSGRCAISPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIEST 60
           MS RC +SPILPT+L AAII  +CGDGIIYK SIRTVS +ENK SNSTITCVTQ      
Sbjct: 1   MSERCTVSPILPTILLAAIIIFLCGDGIIYKASIRTVSINENKNSNSTITCVTQ------ 60

Query: 61  LEEEPLQSSTEYKD-DDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQF 120
              EPL+SS E ++ DD+S T DSL+VP +LS KKARIDWFRKHLPDFRILQSNN TQQF
Sbjct: 61  ---EPLKSSPENREEDDDSGTFDSLIVPFNLSGKKARIDWFRKHLPDFRILQSNNFTQQF 120

Query: 121 DNRLLEFLSHECETQFFMTWISP---------------------------ARTLDSERGY 180
            NRL EFLSH+CETQFFMTWISP                           +RTLDSERGY
Sbjct: 121 HNRLQEFLSHDCETQFFMTWISPTRTFRRRELLAAESIFKSHPRGCLTILSRTLDSERGY 180

Query: 181 KILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAV 240
           KIL+PLLD  FKILAITPDLPSLFKNTPVE WFDEMKRGKKDPG IPLAQNLSNLMRLAV
Sbjct: 181 KILQPLLDHGFKILAITPDLPSLFKNTPVETWFDEMKRGKKDPGQIPLAQNLSNLMRLAV 240

Query: 241 LYRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFME 300
           LY+YGGVYIDTDFIVLKSFMGLKN IGAQSIDP+SK+WT LNNAVLVFDK HPLLEKFME
Sbjct: 241 LYKYGGVYIDTDFIVLKSFMGLKNCIGAQSIDPVSKNWTRLNNAVLVFDKKHPLLEKFME 300

Query: 301 NFASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKP 360
           NFASNFDGSRWGYNGPFLVSRV+AKVTGA A PGFNVT+LPP AFYPVDW KIGELFKKP
Sbjct: 301 NFASNFDGSRWGYNGPFLVSRVIAKVTGAGARPGFNVTILPPAAFYPVDWTKIGELFKKP 360

Query: 361 ENQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSNT 387
            N+A+++WAKA+LAQLN+EAYGIHLWNK SKSY +QKGSVIERLVS HCIIC  T
Sbjct: 361 GNRASANWAKAMLAQLNDEAYGIHLWNKQSKSYTIQKGSVIERLVSAHCIICKYT 406

[Close]

BLAST of MC05g1538 vs. TAIR 10
Match: AT5G01250.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 332.4 bits (851), Expect = 4.8e-91
Identity = 171/358 (47.77%), Postives = 220/358 (61.45%), Query Frame = 0


Query: 54  QVQIESTLEEEPLQSSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSN 113
           +++I+  +   PL S  E       E  D L   + ++EK          L    +   +
Sbjct: 62  KIEIKRVIPHLPLSSERE------GERSDLLKQQTQVNEK----------LQVIEVFSGD 121

Query: 114 NLTQQFDNRLLEFLSHECETQFFMTWISPA---------------------------RTL 173
           NL+ +F  R+ EF+   CE  F MTWISPA                            T+
Sbjct: 122 NLSDKFQKRVNEFVGDGCEVNFVMTWISPADFFGNREVLAIESVFKSHPYGCLMILSATM 181

Query: 174 DSERGYKILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSN 233
           DS +GY  LKP +DR +K+LA+TPDLP L K T  E W DE+K GK+DPG I LAQNLSN
Sbjct: 182 DSPQGYATLKPFIDRGYKVLAVTPDLPFLLKGTAGELWLDEIKSGKRDPGKISLAQNLSN 241

Query: 234 LMRLAVLYRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPL 293
           LMRLA LY+YGGVY+DTD IVLKSF GL+N IGAQ++DP S +WT LNNAVL+FDKNHPL
Sbjct: 242 LMRLAYLYKYGGVYLDTDMIVLKSFKGLRNVIGAQTLDPSSTNWTRLNNAVLIFDKNHPL 301

Query: 294 LEKFMENFASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIG 353
           L KFME FA  F+G+ WGYNGP+LVSRV   V G+    G+N TV+ P  FY V+W +I 
Sbjct: 302 LLKFMEEFAKTFNGNIWGYNGPYLVSRVARAVEGSS---GYNFTVMRPSVFYSVNWLEIK 361

Query: 354 ELFKKPENQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIIC 385
           +LFK P+ +  S W K  L  +    YG+HLWNK S+ Y +++GS + +LVS+HCIIC
Sbjct: 362 KLFKVPKTEKDSKWVKTKLLHMQRNGYGLHLWNKFSRKYEIEQGSAMWKLVSEHCIIC 400

[Close]

BLAST of MC05g1538 vs. TAIR 10
Match: AT2G38150.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 323.6 bits (828), Expect = 2.2e-88
Identity = 164/354 (46.33%), Postives = 219/354 (61.86%), Query Frame = 0


Query: 65  PLQSSTEYKDDDNSE----TLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFD 124
           P+ SS       +SE     LD L+ P   S K  RIDWFR+ LP+  IL+S   ++ F 
Sbjct: 50  PMSSSCNINSVVDSEHKEKELDPLLPPRKAS-KNQRIDWFRRKLPELEILKSTTKSKSFH 109

Query: 125 NRLLEFLSHECETQFFMTWISPA---------------------------RTLDSERGYK 184
            R+L+  +  C  QFFM W+SPA                            +LDS  GY 
Sbjct: 110 TRVLDLYNKNCSAQFFMIWLSPANSFGPREMLAIDTLFTTNPGACLAILSNSLDSPNGYT 169

Query: 185 ILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVL 244
           ILKPL D+ F ++A+T D+P L KNTP EAW   +K G  DPG IPL  NLS+L RLAVL
Sbjct: 170 ILKPLFDQGFNLIAVTIDIPFLVKNTPAEAWLKRLKSGNMDPGSIPLFMNLSDLTRLAVL 229

Query: 245 YRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMEN 304
           Y+YGGVY+DTD I L    GL+N+IGAQS DP +K WT LNNAV+VFD  HPL+ +F++ 
Sbjct: 230 YKYGGVYLDTDIIFLNDMTGLRNAIGAQSSDPATKRWTRLNNAVMVFDIYHPLMREFLQE 289

Query: 305 FASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGF-NVTVLPPVAFYPVDWNKIGELFKKP 364
           +A+ FDG++WGYN P+LVSRV+ ++ G K  PG+ N+T+  P AFYPV+W KI +LFKKP
Sbjct: 290 YATTFDGNKWGYNSPYLVSRVIKRL-GNK--PGYNNLTIFSPDAFYPVNWIKIQKLFKKP 349

Query: 365 ENQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIICSN 387
                + W +  +  +N  +Y IHLWNK ++   +++GSV+  LVS HC +C N
Sbjct: 350 ATTREAKWVEKTVQDMNKGSYMIHLWNKVTRKIKIEEGSVMHTLVSTHCTVCGN 399

[Close]

BLAST of MC05g1538 vs. TAIR 10
Match: AT3G09020.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 313.5 bits (802), Expect = 2.3e-85
Identity = 156/364 (42.86%), Postives = 230/364 (63.19%), Query Frame = 0


Query: 52  VTQVQIESTLEEEPLQSSTEYKD--DDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRI 111
           V  ++I++ +   PL S  E  D  ++N      + V  ++++           L    +
Sbjct: 57  VVTIEIKTIVPYLPLSSEKEVSDQVNNNYSIKQQITVKEEINK-----------LQVLEV 116

Query: 112 LQSNNLTQQFDNRLLEFLSHECETQFFMTWISPAR------------------------- 171
               +++++F  R  EFL  +CE +F MTWISPA                          
Sbjct: 117 FGGKDVSEKFQQRATEFLRDDCEVKFMMTWISPAELFGKREILSVESVFKSHARGCLMIL 176

Query: 172 --TLDSERGYKILKPLLDRAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQ 231
             T+DS +G++ILKP LDR ++++A+TPDLP L K+T  E+W +E++ GK+DPG I LAQ
Sbjct: 177 SSTMDSLQGFRILKPFLDRGYRVMAVTPDLPFLLKDTAGESWLEEIQTGKRDPGKISLAQ 236

Query: 232 NLSNLMRLAVLYRYGGVYIDTDFIVLKSFMGLKNSIGAQSIDPISKSWTILNNAVLVFDK 291
           NLSNLMRLA L+++GGVY+DTD IVLKSF  L+N IGAQ+++P+S++WT LNNAVL+FDK
Sbjct: 237 NLSNLMRLAYLFKFGGVYLDTDMIVLKSFKTLRNVIGAQTLEPVSRNWTRLNNAVLIFDK 296

Query: 292 NHPLLEKFMENFASNFDGSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDW 351
           NHP L K +E FA  F+G+ WG+NGP+LVSRV   V G   T G+N T+L P AFYPV+W
Sbjct: 297 NHPFLLKSIEEFALTFNGNVWGHNGPYLVSRVARAVEG---TDGYNFTILTPPAFYPVNW 356

Query: 352 NKIGELFKKPENQATSSWAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCI 387
            +I +LFK P  +  S   +  + ++   +YG+HLWNK S+ + +++GS +++LVS+ CI
Sbjct: 357 VEIEKLFKVPRTEKDSKRVQVKVLEMQKRSYGLHLWNKFSRKFEIEQGSAMDKLVSNQCI 406

[Close]

BLAST of MC05g1538 vs. TAIR 10
Match: AT2G38152.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 302.0 bits (772), Expect = 7.0e-82
Identity = 171/405 (42.22%), Postives = 229/405 (56.54%), Query Frame = 0


Query: 8   SPILPTVLFAAIIFLVCGDGIIYKVSIRTVSRDENKISNSTITCVTQVQIESTLEEEPLQ 67
           SP+  T +FA I+ LV    I+  +S+++                      S+   E +Q
Sbjct: 25  SPVFSTAIFAVIMLLVVAGTILSNMSLKSTF------------------FWSSPTSEVIQ 84

Query: 68  SSTEYKDDDNSETLDSLVVPSDLSEKKARIDWFRKHLPDFRILQSNNLTQQFDNRLLEFL 127
           +        N     SL  P + + +  RI W   HL +F                    
Sbjct: 85  T--------NRMERKSLAPPKNTTSRD-RIAWLHSHLTEF-------------------- 144

Query: 128 SHECETQFFMTWISPAR---------------------------TLDSERGYKILKPLLD 187
               E +FFMTW SPA                            +LDS +G  ILKPL D
Sbjct: 145 ----EVRFFMTWFSPAEYFGKREMLAVESVFKAHPQGCLMIVSGSLDSLQGDSILKPLND 204

Query: 188 RAFKILAITPDLPSLFKNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVLYRYGGVY 247
           R +K+ A TPD+  L +NTP ++WF EMK  K+DPG IPL QNLSNL RLA LY+YGGVY
Sbjct: 205 RGYKVFAATPDMSLLLENTPAKSWFQEMKSCKRDPGRIPLHQNLSNLARLAFLYKYGGVY 264

Query: 248 IDTDFIVLKSFMGLKNSIGAQS-IDPISKSWTILNNAVLVFDKNHPLLEKFMENFASNFD 307
           +DTDFIV +SF GLKNSIGAQ+ ++  SK+WT LNNAVL+F+K+HPL+  F+E FAS FD
Sbjct: 265 LDTDFIVTRSFKGLKNSIGAQTVVEGDSKNWTRLNNAVLIFEKDHPLVYSFIEEFASTFD 324

Query: 308 GSRWGYNGPFLVSRVVAKVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPENQATSS 367
           G++WG+NGP+LV+RV  +   A+ T G N TVLPPVAFYP +W  I  LF+ P     S+
Sbjct: 325 GNKWGHNGPYLVTRVAQR---ARETIGDNFTVLPPVAFYPFNWLDIPRLFQTPRGSNDST 375

Query: 368 WAKAVLAQLNNEAYGIHLWNKHSKSYIMQKGSVIERLVSDHCIIC 385
             K  L +LN E+YG+HLWNK ++   + KGSVI+ ++SDHC++C
Sbjct: 385 LLKTDLVKLNRESYGLHLWNKITRKLKIGKGSVIDIIISDHCVVC 375

[Close]

BLAST of MC05g1538 vs. TAIR 10
Match: AT1G61050.1 (alpha 1,4-glycosyltransferase family protein )

HSP 1 Score: 278.5 bits (711), Expect = 8.2e-75
Identity = 151/391 (38.62%), Postives = 223/391 (57.03%), Query Frame = 0


Query: 42  NKISNSTITCVTQVQIESTLEEEPLQSSTEYKDDDNSETLDS--LVVPSDLS---EKKAR 101
           N  S  ++  V    I+STL    LQ    ++   +S   DS  L+V  + S    +K  
Sbjct: 43  NSFSVFSLHLVPNQPIQSTLSPTHLQ-ILHHQTSTSSSVSDSSLLLVVKETSLGFIQKQN 102

Query: 102 IDWFRKHLPDFRILQSNNLT------------QQFDNRLLEFLS-HECETQFFMTWISP- 161
           +   R      R  +S  LT            Q+F  R+   LS   CE+ FFMTWIS  
Sbjct: 103 VSSTRIEKKTRRFKRSTELTPAITQRLQVKSRQRFQTRVKSLLSKSSCESLFFMTWISSI 162

Query: 162 --------------------------ARTLDSERGYKILKPLLDRAFKILAITPDLPSLF 221
                                     + + D +RG  ILKP  D+  K+L I PD   +F
Sbjct: 163 ESFGDRERFTIESLFKFHPNGCLILVSNSFDCDRGTLILKPFTDKGLKVLPIKPDFAYIF 222

Query: 222 KNTPVEAWFDEMKRGKKDPGLIPLAQNLSNLMRLAVLYRYGGVYIDTDFIVLKSFMGLKN 281
           K+T  E WF+ +K+G   PG+IPL QNLSNL+RL +LY+YGG+Y+DTD I+LKS   L N
Sbjct: 223 KDTSAEKWFERLKKGTLSPGVIPLEQNLSNLLRLVLLYKYGGIYLDTDVIILKSLSNLHN 282

Query: 282 SIGAQSIDPISKSWTILNNAVLVFDKNHPLLEKFMENFASNFDGSRWGYNGPFLVSRVVA 341
            IGAQ++DP++K W+ LNNAVL+FDKNHPLL++F++ F+  F+G++WG+NGP+LVSRV+ 
Sbjct: 283 VIGAQTVDPVTKKWSRLNNAVLIFDKNHPLLKRFIDEFSRTFNGNKWGHNGPYLVSRVIT 342

Query: 342 KVTGAKATPGFNVTVLPPVAFYPVDWNKIGELFKKPENQATSSWAKAVLAQLNNEAYGIH 388
           ++    ++     +VLPP AFYPVDW +I   ++ P N+ + +W +  L  L    + +H
Sbjct: 343 RIK-ISSSSDLGFSVLPPSAFYPVDWTRIKGFYRAPTNE-SDAWLRKRLTHLRKNTFAVH 402

The following BLAST results are available for this feature:

BLAST of MC05g1538 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q67BJ4	2.1e-30	35.62	Lactosylceramide 4-alpha-galactosyltransferase OS=Mus musculus OX=10090 GN=A4gal...	[more]
Q9JI93	1.3e-29	35.75	Lactosylceramide 4-alpha-galactosyltransferase OS=Rattus norvegicus OX=10116 GN=...	[more]
Q9N289	7.4e-28	35.68	Lactosylceramide 4-alpha-galactosyltransferase (Fragment) OS=Pongo pygmaeus OX=9...	[more]
Q9NPC4	2.1e-27	35.18	Lactosylceramide 4-alpha-galactosyltransferase OS=Homo sapiens OX=9606 GN=A4GALT...	[more]
Q9N291	2.8e-27	35.18	Lactosylceramide 4-alpha-galactosyltransferase OS=Pan troglodytes OX=9598 GN=A4G...	[more]

BLAST of MC05g1538 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145489.1	5.01e-277	93.24	lactosylceramide 4-alpha-galactosyltransferase [Momordica charantia]	[more]
XP_016899682.1	1.31e-233	78.74	PREDICTED: lactosylceramide 4-alpha-galactosyltransferase [Cucumis melo] >KAA004...	[more]
XP_004137912.1	3.42e-229	77.29	lactosylceramide 4-alpha-galactosyltransferase [Cucumis sativus] >KGN58777.1 hyp...	[more]
XP_022948708.1	1.34e-224	76.87	lactosylceramide 4-alpha-galactosyltransferase-like [Cucurbita moschata]	[more]
XP_022971397.1	1.09e-223	75.66	lactosylceramide 4-alpha-galactosyltransferase-like isoform X2 [Cucurbita maxima...	[more]

BLAST of MC05g1538 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CVE1	2.43e-277	93.24	lactosylceramide 4-alpha-galactosyltransferase OS=Momordica charantia OX=3673 GN...	[more]
A0A5A7TRE8	6.35e-234	78.74	Lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S4DUL6	6.35e-234	78.74	lactosylceramide 4-alpha-galactosyltransferase OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A0A0LDG3	1.65e-229	77.29	Gb3_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731870 P...	[more]
A0A6J1GA34	6.47e-225	76.87	lactosylceramide 4-alpha-galactosyltransferase-like OS=Cucurbita moschata OX=366...	[more]

BLAST of MC05g1538 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01250.1	4.8e-91	47.77	alpha 1,4-glycosyltransferase family protein	[more]
AT2G38150.1	2.2e-88	46.33	alpha 1,4-glycosyltransferase family protein	[more]
AT3G09020.1	2.3e-85	42.86	alpha 1,4-glycosyltransferase family protein	[more]
AT2G38152.1	7.0e-82	42.22	alpha 1,4-glycosyltransferase family protein	[more]
AT1G61050.1	8.2e-75	38.62	alpha 1,4-glycosyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007577	Glycosyltransferase, DXD sugar-binding motif	PFAM	PF04488	Gly_transf_sug	coord: 132..242 e-value: 8.1E-20 score: 71.3
None	No IPR available	GENE3D	3.90.550.20		coord: 92..311 e-value: 1.6E-22 score: 82.3
None	No IPR available	PANTHER	PTHR46781:SF2	ALPHA 1,4-GLYCOSYLTRANSFERASE FAMILY PROTEIN	coord: 8..144
None	No IPR available	PANTHER	PTHR46781:SF2	ALPHA 1,4-GLYCOSYLTRANSFERASE FAMILY PROTEIN	coord: 144..386
IPR007652	Alpha 1,4-glycosyltransferase domain	PFAM	PF04572	Gb3_synth	coord: 260..386 e-value: 3.0E-31 score: 108.3
IPR044789	Putative alpha 1,4-glycosyltransferase, plant	PANTHER	PTHR46781	ALPHA 1,4-GLYCOSYLTRANSFERASE FAMILY PROTEIN	coord: 144..386
IPR044789	Putative alpha 1,4-glycosyltransferase, plant	PANTHER	PTHR46781	ALPHA 1,4-GLYCOSYLTRANSFERASE FAMILY PROTEIN	coord: 8..144
IPR029044	Nucleotide-diphospho-sugar transferases	SUPERFAMILY	53448	Nucleotide-diphospho-sugar transferases	coord: 96..384

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g1538.1	MC05g1538.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016757	glycosyltransferase activity