MC05g1525 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g1525 vs. ExPASy Swiss-Prot
Match: Q9SS80 (Protein OCTOPUS OS=Arabidopsis thaliana OX=3702 GN=OPS PE=1 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 3.8e-138
Identity = 359/729 (49.25%), Postives = 434/729 (59.53%), Query Frame = 0

Query: 1   MNPSTD-----AQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS-- 60
           MNP+TD     A    PPP PP PHR + +C RHP E FTGFCP CLCERLS+LD ++  
Sbjct: 1   MNPATDPVSAAAAALAPPPQPPQPHRLSTSCNRHPEERFTGFCPSCLCERLSVLDQTNNG 60

Query: 61  -SSSSAKKPPSTAAAALKAIFRPA---SAALPSTSNRSTNRPSFFPELRRTKSFSFSASR 120
            SSSS+KKPP+ +AAALKA+F+P+        +T+     +P FFPELRRTKSFS ++  
Sbjct: 61  GSSSSSKKPPTISAAALKALFKPSGNNGVGGVNTNGNGRVKPGFFPELRRTKSFS-ASKN 120

Query: 121 NDAFSASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVA-VAGAQISVESRNFEEANSGLGG 180
           N+ FS  FEPQR+SCDVR+R++L +LFSQ+   +  + V G +I VE R      S +  
Sbjct: 121 NEGFSGVFEPQRRSCDVRLRSSLWNLFSQDEQRNLPSNVTGGEIDVEPR-----KSSVAE 180

Query: 181 PVLEV-QEGEIRVSEEP----------NAVDVVIENGVQEIVEEGEEEI-EAELEAESV- 240
           PVLEV  EGE    +E            A D  I N   E++ E  +EI E   E E   
Sbjct: 181 PVLEVNDEGEAESDDEELEEEEEEDYVEAGDFEILNDSGELMREKSDEIVEVREEIEEAV 240

Query: 241 -------QQELKTMKDHIDLDSNAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEK 300
                  ++ELK +KD+IDLDS  KKPS      +  SFWSAASVFSKKLQKWR  QK K
Sbjct: 241 KPTKGLSEEELKPIKDYIDLDSQTKKPS------VRRSFWSAASVFSKKLQKWRQNQKMK 300

Query: 301 KQRNGS----GSTTLPVEKPIGRHFRDTQSEIADYGFGRRSCDTDPRFSLDA-------- 360
           K+RNG     GS  LPVEKPIGR  RDTQSEIADYG+GRRSCDTDPRFSLDA        
Sbjct: 301 KRRNGGDHRPGSARLPVEKPIGRQLRDTQSEIADYGYGRRSCDTDPRFSLDAGRFSLDAG 360

Query: 361 ------GRMSFDDPRYSFDEPRASWDGYLISRTF-------PKMPTMLSVVEDAP----I 420
                 GR+S DDPRYSFDEPRASWDG LI RT        P  P+MLSVVEDAP     
Sbjct: 361 RFSVDIGRISLDDPRYSFDEPRASWDGSLIGRTMFPPAARAPPPPSMLSVVEDAPPPVHR 420

Query: 421 NVFRSDTQIPVEE---------SPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDR-SN 480
           +V R+D Q PVEE           N  ++   +PGGS QTRDYY+D SSRRRKSLDR S+
Sbjct: 421 HVTRADMQFPVEEPAPPPPVVNQTNGVSDPVIIPGGSIQTRDYYTDSSSRRRKSLDRSSS 480

Query: 481 SLRKTAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDS 540
           S+RKTAAAVVA++DE K SVS+A     + D   G    + D ++ A    D+ S    +
Sbjct: 481 SMRKTAAAVVADMDEPKLSVSSA----ISIDAYSG---SLRDNNNYAVETADNGSFREPA 540

Query: 541 GFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNK-----DEEEDRESRPNG--VERS 600
             I     N  + +KKSRRWGK W+I GLI R+  NK     +EEEDR  R NG  VERS
Sbjct: 541 MMIGDRKVNSNDNNKKSRRWGK-WSILGLIYRKSVNKYEEEEEEEEDRYRRLNGGMVERS 600

Query: 601 YSESWPELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGK 641
            SESWPELR      G GG  P+M RSNS+VSWRSS                    G G 
Sbjct: 601 LSESWPELR----NGGGGGGGPRMVRSNSNVSWRSS--------------------GGGS 660

BLAST of MC05g1525 vs. ExPASy Swiss-Prot
Match: Q9LFB9 (Protein OCTOPUS-like OS=Arabidopsis thaliana OX=3702 GN=OPSL1 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 2.4e-108
Identity = 320/684 (46.78%), Postives = 390/684 (57.02%), Query Frame = 0

Query: 1   MNPSTDAQP--APPPPLPP-LPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSSS--- 60
           MN S D  P  A     PP  PHR + +C  HP E F+GFCP CLC+RLS+LD +++   
Sbjct: 1   MNLSADQAPVTAVDELAPPSQPHRLSTSCDLHPEERFSGFCPSCLCDRLSVLDHNAAPPP 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRSTNRPSFFPELRRTKSFSFSASRNDAFS 120
           SSS++KPPS +A +LKA+F+P+S+   +++     RP FFPELRRTK  SFSA  N+ FS
Sbjct: 61  SSSSRKPPSISAVSLKALFKPSSSGTNNSNGNGRVRPGFFPELRRTK--SFSAKNNEGFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAGAQISVESRNFEEANSGLGGPVLEV- 180
             FEPQR+SCDVR+R+   +L   E +           SV+    E   S +   VLEV 
Sbjct: 121 GGFEPQRRSCDVRLRDDERNLPINEAA-----------SVDKIEEEARESSVSEIVLEVT 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
           +E EI   EE        E    EIVEE   EI  E      ++ELK MKD++DL S  K
Sbjct: 181 EEAEIEEDEENG------EKDPGEIVEEKSSEIGEE------EEELKPMKDYMDLYSQTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPS   +K+ AGSF+SAASVFSKKLQKW+ KQK KK RNG G          GR     Q
Sbjct: 241 KPS---VKDFAGSFFSAASVFSKKLQKWKQKQKVKKPRNGVGG---------GR----PQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDA-------GRMSFDDPRYSFDEPRASWDGYLISRT--- 360
           SEI   G GRRS DTDPRFSLDA       GR+S DD RYS DEPRASWDG+LI RT   
Sbjct: 301 SEI---GVGRRSSDTDPRFSLDAGRFSVDIGRISMDDSRYSLDEPRASWDGHLIGRTTAA 360

Query: 361 -FPKMPTMLSVVEDAPINVFRSDTQIPVEESPNSTNEDEN----VPGGSSQTRDYYS--D 420
             P  P+MLSVVE+AP+N  RSD QIP   S    + D +    +PGGS+QTRDYY+   
Sbjct: 361 RVPLPPSMLSVVENAPLN--RSDMQIPSSPSIKPISGDSDPIIIIPGGSNQTRDYYTGPP 420

Query: 421 SSRRRKSLDRSNSLRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDSNANP 480
           SSRRRKSLDRSNS+RK    +V E++++KSVSN     +TT I           DSN+  
Sbjct: 421 SSRRRKSLDRSNSIRK----IVTELEDVKSVSN-----STTTI-----------DSNSME 480

Query: 481 LRDDCSGSLDSGFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRESRPNG 540
             ++              GN+  + KKSRRWGK W+I G I R+ G  DEEEDR SR N 
Sbjct: 481 TAEN-------------KGNQNGD-KKSRRWGK-WSILGFIYRK-GKDDEEEDRYSRSNS 540

Query: 541 ---VERSYSESWPELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAES 600
              VERS SESWPE+R     +G GG  PKM RSNS+VSWRSS                 
Sbjct: 541 AGMVERSLSESWPEMR-----NGEGG-GPKMRRSNSNVSWRSS----------------- 568

Query: 601 NGNGNGKKKKEEPVLERNRSARYSPTNVDNGLLRFYLTPMR-------GSRRGGSG---- 641
            G G+           RN+S+RYS  + +NG+LRFYLTPMR       GS  GG G    
Sbjct: 601 -GGGSA----------RNKSSRYSSKDGENGMLRFYLTPMRRSWKTSGGSGGGGGGGGGG 568

BLAST of MC05g1525 vs. NCBI nr
Match: XP_038903136.1 (protein OCTOPUS-like [Benincasa hispida])

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 522/655 (79.69%), Postives = 567/655 (86.56%), Query Frame = 0

Query: 1   MNPSTDAQPAPPP---PLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSSS--- 60
           MNPSTD QPAPPP   PLPPLPHRP+A CPRHP EHFT FCPLCLCERLS+LDSS+S   
Sbjct: 1   MNPSTDEQPAPPPLPPPLPPLPHRPSAPCPRHPQEHFTAFCPLCLCERLSVLDSSASAAS 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRSTNRPS-FFPELRRTKSFSFSASRNDAF 120
           SSS++KP STAA+AL+AIFRPA            NRPS FFPELRR KSFS  AS+N+AF
Sbjct: 61  SSSSRKPHSTAASALRAIFRPAP----------PNRPSSFFPELRRAKSFS--ASKNEAF 120

Query: 121 SASFEPQRKSCDVRVRNTLCSLFSQEPSTSR--VAVAGAQISVESRNFEEANSGLGGPVL 180
           SA FEPQRKSCDVR+RNTLCSLFSQ+ S S   +A  G +I +ES+N E+  S    P  
Sbjct: 121 SAVFEPQRKSCDVRLRNTLCSLFSQDASASSRLLAAPGPEIILESKNLEDPISSCLEPQP 180

Query: 181 EVQEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQ--QELKTMKDHIDLD 240
           +  +GEIRVS +PN VDVVIENGVQEIVEE EEEI+ EL +ESVQ  +E KTMKDHIDLD
Sbjct: 181 D-GDGEIRVSGQPNVVDVVIENGVQEIVEEEEEEIQVELGSESVQLQEEFKTMKDHIDLD 240

Query: 241 SNAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHF 300
           S+ KKP+GR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHF
Sbjct: 241 SHTKKPAGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHF 300

Query: 301 RDTQSEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPT 360
           R+TQSEIADYGFGRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFP+MPT
Sbjct: 301 RETQSEIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPT 360

Query: 361 MLSVVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSDSS-RRRKSLDRSN 420
           MLSVVEDAPI+VFRSDTQIPVE+S NSTNEDEN+PGGSSQTRDYYSDSS RRRKSLDRSN
Sbjct: 361 MLSVVEDAPIHVFRSDTQIPVEDSINSTNEDENIPGGSSQTRDYYSDSSSRRRKSLDRSN 420

Query: 421 SLRKTAAAVVAEIDEMKS-VSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDS 480
           S+RKTAAAVVAEID+MKS VSNAKVSPATTD+CHGPKL IPDRDSN+N +RDDCSGS D 
Sbjct: 421 SIRKTAAAVVAEIDDMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSNSMRDDCSGSFDD 480

Query: 481 GFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESWP 540
                 +GNRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRES RPNGVERSYSESWP
Sbjct: 481 AASVVGTGNRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWP 540

Query: 541 ELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIG-GPFSSSRKSNAESNGNGNGKKKKEE 600
           ELRG+R+GD +GGFNPKMFRSNSSVSWRSSSM+G G FSSSRKSNAESNGNG  KK++ +
Sbjct: 541 ELRGDRNGDVKGGFNPKMFRSNSSVSWRSSSMMGSGYFSSSRKSNAESNGNGKKKKEEPQ 600

Query: 601 PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 640
           PVLERNRSAR+SPTNVDNGLLRFYLTP+RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 PVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRLY 636

BLAST of MC05g1525 vs. NCBI nr
Match: XP_022934120.1 (UPF0503 protein At3g09070, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 927 bits (2395), Expect = 0.0
Identity = 518/654 (79.20%), Postives = 559/654 (85.47%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS--SSSSA 60
           MNPST  QPAPPPPLPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS  SSSS 
Sbjct: 1   MNPSTHEQPAPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSSSST 60

Query: 61  KKPPSTAAAALKAIFRPASAALPSTSNRSTNRPS-FFPELRRTKSFSFSASRNDAFSASF 120
           +KP STAA+ALKAIFRPA            NRPS FFPELRRTKSFS  AS+NDAFSA F
Sbjct: 61  RKPHSTAASALKAIFRPAP----------PNRPSSFFPELRRTKSFS--ASKNDAFSAVF 120

Query: 121 EPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAG-AQISVESRNFEEANSGLGGPVLE---V 180
           EPQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S      LE   V
Sbjct: 121 EPQRKSCDVRVRNAHCSLFSHDASASSNLVAPPAEITPETKILEDSSSSC----LESHPV 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
            +GEI+VS+ PN VDVVIENGVQEIVEE E  +E   E   +Q+E KTMKDHIDLDS+ K
Sbjct: 181 GDGEIKVSKRPNVVDVVIENGVQEIVEEEEIRVEVGPEPVQLQEEFKTMKDHIDLDSHTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFRDTQ
Sbjct: 241 KPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRDTQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPTMLSV 360
           SEIADYG+GRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFP+MPTMLSV
Sbjct: 301 SEIADYGYGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSV 360

Query: 361 VEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSDSS-RRRKSLDRSNSLRK 420
           VEDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSDSS RRRKSLDRSNS+RK
Sbjct: 361 VEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRK 420

Query: 421 TAAAVVAEIDEMKS-VSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFID 480
           TAAAVVAEIDEMKS VSNAKVSPATTD+CHGPKL IPDRDSN++ L++DCS S DS F D
Sbjct: 421 TAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSFDSAFND 480

Query: 481 AAS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESWP 540
           AAS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+ES RPNG+ERSYS SWP
Sbjct: 481 AASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWP 540

Query: 541 ELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGKKKKEEP 600
           ELRGER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSNAE+NGNG  +KK EEP
Sbjct: 541 ELRGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAETNGNG--RKKNEEP 600

Query: 601 VLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 640
           VLERNRSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 VLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 630

BLAST of MC05g1525 vs. NCBI nr
Match: XP_023526761.1 (UPF0503 protein At3g09070, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 924 bits (2387), Expect = 0.0
Identity = 518/654 (79.20%), Postives = 560/654 (85.63%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPP---LPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS--SS 60
           MNPST  +PAPPPP   LPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS  SS
Sbjct: 1   MNPSTHEKPAPPPPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSS 60

Query: 61  SSAKKPPSTAAAALKAIFRPASAALPSTSNRSTNRPSFF-PELRRTKSFSFSASRNDAFS 120
           SS +KP STAA+ALKAIFRPA            NRPSFF PELRRTKSFS  AS+NDAFS
Sbjct: 61  SSTRKPHSTAASALKAIFRPAP----------PNRPSFFLPELRRTKSFS--ASKNDAFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAG-AQISVESRNFEEANSGLGGPVLEV 180
           A FEPQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S    P   V
Sbjct: 121 AVFEPQRKSCDVRVRNAHCSLFSHDASASSNIVAPPAEITSETKILEDSSSSCLEPH-PV 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
            +GEIRVS++PN VDVV ENGVQEIVEE E ++E   E   +Q+E KTMKDHIDLDS+ K
Sbjct: 181 GDGEIRVSKQPNVVDVVNENGVQEIVEEEEIQVEVGPEPVQLQEEFKTMKDHIDLDSHTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFRDTQ
Sbjct: 241 KPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGVGSTTLPVEKPIGRHFRDTQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPTMLSV 360
           SEIADYG+GRRSCD DPRFSLDAGR+SFDDPRYSFDEPRASWDGYLISRTFP+MPTMLSV
Sbjct: 301 SEIADYGYGRRSCDIDPRFSLDAGRISFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSV 360

Query: 361 VEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSDSS-RRRKSLDRSNSLRK 420
           VEDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSDSS RRRKSLDRSNS+RK
Sbjct: 361 VEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRK 420

Query: 421 TAAAVVAEIDEMKS-VSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFID 480
           TAAAVVAEIDEMKS VSNAKVSPATTD+CHGPKL IPDRDSN++ L+DDCS SLDS F D
Sbjct: 421 TAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQDDCSKSLDSAFND 480

Query: 481 AAS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESWP 540
           AAS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+ES RPNG+ERSYS SWP
Sbjct: 481 AASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWP 540

Query: 541 ELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGKKKKEEP 600
           ELRGER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSNAE+NGNG  KKK EEP
Sbjct: 541 ELRGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAETNGNG--KKKNEEP 600

Query: 601 VLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 640
           VLERNRSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 VLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 633

BLAST of MC05g1525 vs. NCBI nr
Match: KAG6580491.1 (Protein OCTOPUS, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 924 bits (2387), Expect = 0.0
Identity = 518/655 (79.08%), Postives = 559/655 (85.34%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPP----LPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS--S 60
           MNPST  QPAPPPP    LPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS  S
Sbjct: 1   MNPSTHEQPAPPPPPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTS 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRSTNRPS-FFPELRRTKSFSFSASRNDAF 120
           SSS +KP STAA+ALKAIFRPA            NRPS FFPELRRTKSFS  AS+NDAF
Sbjct: 61  SSSTRKPHSTAASALKAIFRPAP----------PNRPSSFFPELRRTKSFS--ASKNDAF 120

Query: 121 SASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAG-AQISVESRNFEEANSGLGGPVLE 180
           SA FEPQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S    P   
Sbjct: 121 SAVFEPQRKSCDVRVRNAHCSLFSHDASASSNLVAPPAEITPETKILEDSSSSCLEPY-P 180

Query: 181 VQEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNA 240
           V +GEIRVS++PN VDVVIENGVQEIVEE E ++E   E   +Q+E KTMKDHIDLDS+ 
Sbjct: 181 VGDGEIRVSKQPNVVDVVIENGVQEIVEEEEIQVEVGPEPVQLQEEFKTMKDHIDLDSHT 240

Query: 241 KKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDT 300
           KKPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFRDT
Sbjct: 241 KKPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGVGSTTLPVEKPIGRHFRDT 300

Query: 301 QSEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPTMLS 360
           QSEIADYG+GRRSCD DPRFSLDAGRMS DDPRYSFDEPRASWDGYLISRT P+MPTMLS
Sbjct: 301 QSEIADYGYGRRSCDIDPRFSLDAGRMSIDDPRYSFDEPRASWDGYLISRTLPRMPTMLS 360

Query: 361 VVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSDSS-RRRKSLDRSNSLR 420
           VVEDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSDSS RRRKSLDRSNS+R
Sbjct: 361 VVEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIR 420

Query: 421 KTAAAVVAEIDEMKS-VSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFI 480
           KTAAAVVAEIDEMKS VSNAKVSPATTD+CHGPKL IPDRDSN++ L++DCS SLDS F 
Sbjct: 421 KTAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSLDSAFN 480

Query: 481 DAAS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESW 540
           DAAS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+ES RPNG+ERSYS SW
Sbjct: 481 DAASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSW 540

Query: 541 PELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGKKKKEE 600
           PELRGER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSNAE+NGNG  KKK EE
Sbjct: 541 PELRGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAETNGNG--KKKNEE 600

Query: 601 PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 640
           PVLERNRSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 PVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 634

BLAST of MC05g1525 vs. NCBI nr
Match: KAG7017244.1 (UPF0503 protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 920 bits (2379), Expect = 0.0
Identity = 517/655 (78.93%), Postives = 558/655 (85.19%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPP----LPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS--S 60
           MNPST  QPAPPPP    LPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS  S
Sbjct: 1   MNPSTHEQPAPPPPPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTS 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRSTNRPS-FFPELRRTKSFSFSASRNDAF 120
           SSS +KP STAA+ALKAIFRPA            NRPS FFPELRRTKSFS  AS+NDAF
Sbjct: 61  SSSTRKPHSTAASALKAIFRPAP----------PNRPSSFFPELRRTKSFS--ASKNDAF 120

Query: 121 SASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAG-AQISVESRNFEEANSGLGGPVLE 180
           SA FEPQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S    P   
Sbjct: 121 SAVFEPQRKSCDVRVRNAHCSLFSHDASASSNLVAPPAEITPETKILEDSSSSCLEPY-P 180

Query: 181 VQEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNA 240
           V +GEIRVS++PN VDVVIENGVQEIVEE E ++E   E   +Q+E KTMKDHIDLDS+ 
Sbjct: 181 VGDGEIRVSKQPNVVDVVIENGVQEIVEEEEIQVEVGPEPVQLQEEFKTMKDHIDLDSHT 240

Query: 241 KKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDT 300
           KKPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFRDT
Sbjct: 241 KKPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGVGSTTLPVEKPIGRHFRDT 300

Query: 301 QSEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPTMLS 360
           QSEIADYG+GRRSCD DPRFSLDAGRMS DDPRYSFDEPRASWDGYLISRT P+MPTMLS
Sbjct: 301 QSEIADYGYGRRSCDIDPRFSLDAGRMSIDDPRYSFDEPRASWDGYLISRTLPRMPTMLS 360

Query: 361 VVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSDSS-RRRKSLDRSNSLR 420
           VVEDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSDSS RRRKSLDRSNS+R
Sbjct: 361 VVEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIR 420

Query: 421 KTAAAVVAEIDEMKS-VSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFI 480
           KTAAAVVAEIDEMKS VSNAKVSPATTD+CHGPKL IPDRDSN++ L++DCS SLDS F 
Sbjct: 421 KTAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSLDSAFN 480

Query: 481 DAAS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESW 540
           DAAS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+ES RPNG+ERSYS SW
Sbjct: 481 DAASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESGRPNGMERSYSGSW 540

Query: 541 PELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGKKKKEE 600
           PEL GER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSNAE+NGNG  KKK EE
Sbjct: 541 PELCGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAETNGNG--KKKNEE 600

Query: 601 PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 640
           PVLERNRSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 PVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 634

BLAST of MC05g1525 vs. ExPASy TrEMBL
Match: A0A6J1F6S2 (UPF0503 protein At3g09070, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441390 PE=4 SV=1)

HSP 1 Score: 927 bits (2395), Expect = 0.0
Identity = 518/654 (79.20%), Postives = 559/654 (85.47%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS--SSSSA 60
           MNPST  QPAPPPPLPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS  SSSS 
Sbjct: 1   MNPSTHEQPAPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSSSST 60

Query: 61  KKPPSTAAAALKAIFRPASAALPSTSNRSTNRPS-FFPELRRTKSFSFSASRNDAFSASF 120
           +KP STAA+ALKAIFRPA            NRPS FFPELRRTKSFS  AS+NDAFSA F
Sbjct: 61  RKPHSTAASALKAIFRPAP----------PNRPSSFFPELRRTKSFS--ASKNDAFSAVF 120

Query: 121 EPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAG-AQISVESRNFEEANSGLGGPVLE---V 180
           EPQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S      LE   V
Sbjct: 121 EPQRKSCDVRVRNAHCSLFSHDASASSNLVAPPAEITPETKILEDSSSSC----LESHPV 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
            +GEI+VS+ PN VDVVIENGVQEIVEE E  +E   E   +Q+E KTMKDHIDLDS+ K
Sbjct: 181 GDGEIKVSKRPNVVDVVIENGVQEIVEEEEIRVEVGPEPVQLQEEFKTMKDHIDLDSHTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFRDTQ
Sbjct: 241 KPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRDTQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPTMLSV 360
           SEIADYG+GRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFP+MPTMLSV
Sbjct: 301 SEIADYGYGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSV 360

Query: 361 VEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSDSS-RRRKSLDRSNSLRK 420
           VEDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSDSS RRRKSLDRSNS+RK
Sbjct: 361 VEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRK 420

Query: 421 TAAAVVAEIDEMKS-VSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFID 480
           TAAAVVAEIDEMKS VSNAKVSPATTD+CHGPKL IPDRDSN++ L++DCS S DS F D
Sbjct: 421 TAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSFDSAFND 480

Query: 481 AAS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESWP 540
           AAS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+ES RPNG+ERSYS SWP
Sbjct: 481 AASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWP 540

Query: 541 ELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGKKKKEEP 600
           ELRGER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSNAE+NGNG  +KK EEP
Sbjct: 541 ELRGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAETNGNG--RKKNEEP 600

Query: 601 VLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 640
           VLERNRSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 VLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 630

BLAST of MC05g1525 vs. ExPASy TrEMBL
Match: A0A6J1J5H7 (UPF0503 protein At3g09070, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481925 PE=4 SV=1)

HSP 1 Score: 918 bits (2373), Expect = 0.0
Identity = 515/655 (78.63%), Postives = 555/655 (84.73%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS---SSSS 60
           MNPST  QPAPPPPLPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS   SSSS
Sbjct: 1   MNPSTHEQPAPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSSSSS 60

Query: 61  AKKPPSTAAAALKAIFRPASAALPSTSNRSTNRPS-FFPELRRTKSFSFSASRNDAFSAS 120
            +KP STAA+ALKAIFRPA            NRPS FFPELRRTKSFS  AS+NDAFSA 
Sbjct: 61  TRKPHSTAASALKAIFRPAP----------PNRPSSFFPELRRTKSFS--ASKNDAFSAV 120

Query: 121 FEPQRKSCDVRVRNTLCSLFSQEPSTSRVAVA-GAQISVESRNFEEANSGLGGPVLEVQ- 180
           FEPQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S      LE Q 
Sbjct: 121 FEPQRKSCDVRVRNAHCSLFSHDASASSNLVAPSAEITPETKFLEDSSSSC----LEHQP 180

Query: 181 --EGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNA 240
               EIRVSE+PN VDVVIENG QEIVEE E ++E   E   +Q+E KTMKDHIDLDS+ 
Sbjct: 181 LGHREIRVSEQPNVVDVVIENGAQEIVEEKEIQVEVGPETVQLQEEFKTMKDHIDLDSHT 240

Query: 241 KKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDT 300
           KKPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GST LPVEKPIGRHFRDT
Sbjct: 241 KKPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTALPVEKPIGRHFRDT 300

Query: 301 QSEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPTMLS 360
           QSEIADYG+GRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFP+MPTMLS
Sbjct: 301 QSEIADYGYGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLS 360

Query: 361 VVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSDSS-RRRKSLDRSNSLR 420
           VVEDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSDSS RRRKSLDRSNS+R
Sbjct: 361 VVEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIR 420

Query: 421 KTAAAVVAEIDEMKS-VSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFI 480
           KTAAAVVAEIDEMKS VSNAKVSPATTD+CHGPKL IPDRDSN++ L++DCS SLDS F 
Sbjct: 421 KTAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSLDSAFN 480

Query: 481 DAAS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESW 540
           D AS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+ES RPNG+ERS S SW
Sbjct: 481 DVASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSNSGSW 540

Query: 541 PELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGKKKKEE 600
           PELRGER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSN E+NGNG  KKK EE
Sbjct: 541 PELRGERNSDIKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNTETNGNG--KKKNEE 600

Query: 601 PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 640
           PVLER+RSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 PVLERSRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 631

BLAST of MC05g1525 vs. ExPASy TrEMBL
Match: A0A5A7TLQ7 (UPF0503 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001040 PE=4 SV=1)

HSP 1 Score: 914 bits (2361), Expect = 0.0
Identity = 510/655 (77.86%), Postives = 559/655 (85.34%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS------S 60
           MNPSTD QP PPP LPPLPHRP+A CPRHP EHFT FCPLCLCERLSLLDSSS      S
Sbjct: 1   MNPSTDGQPLPPP-LPPLPHRPSATCPRHPQEHFTAFCPLCLCERLSLLDSSSAAAASSS 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRSTNRPS-FFPELRRTKSFSFSASRNDAF 120
           SSS++KP STAA+ALKAIFRP             NRPS FFPELRRTKSFS  AS+N+AF
Sbjct: 61  SSSSRKPHSTAASALKAIFRPPP----------PNRPSSFFPELRRTKSFS--ASKNEAF 120

Query: 121 SASFEPQRKSCDVRVRNTLCSLFSQEPSTSR--VAVAGAQISVESRNFEEANSGLGGPVL 180
           S  FEPQRKSCDVR+RNTLCSL SQ+ S+S   +A A  +I VE++N E+  S    P+ 
Sbjct: 121 STVFEPQRKSCDVRLRNTLCSLISQDASSSSKVLAPAAPEIVVETKNLEDPCSSWVEPIP 180

Query: 181 EVQEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESV--QQELKTMKDHIDLD 240
           +  +G+IRVS +PN  D VIEN VQEIV   EEEI+ ELE+ESV  Q+E KTMKDHIDLD
Sbjct: 181 D-GDGDIRVSGQPNVGDFVIENSVQEIV---EEEIQVELESESVHLQEEFKTMKDHIDLD 240

Query: 241 SNAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHF 300
           S+ KKPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHF
Sbjct: 241 SHTKKPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHF 300

Query: 301 RDTQSEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPT 360
           R+TQSEIADYGFGRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFP+MPT
Sbjct: 301 RETQSEIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPT 360

Query: 361 MLSVVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSDSS-RRRKSLDRSN 420
           MLSVVEDAPI+VFRSDTQIPVE+S NSTNE+EN+PGGSSQTR+YYSDSS RRRKSLDRSN
Sbjct: 361 MLSVVEDAPIHVFRSDTQIPVEDSINSTNEEENIPGGSSQTREYYSDSSSRRRKSLDRSN 420

Query: 421 SLRKTAAAVVAEIDEMKS-VSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDS 480
           S+RKTAAAVVAEID+MKS VSNAKVSPATTD+CH PKLG+PDRDSN+N LRDD SGS + 
Sbjct: 421 SIRKTAAAVVAEIDDMKSSVSNAKVSPATTDVCHVPKLGLPDRDSNSNSLRDDYSGSFED 480

Query: 481 GFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESWP 540
                 + NRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRES RPNGVERSYSESWP
Sbjct: 481 AASVVGTANRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWP 540

Query: 541 ELRGERSGDGR-GGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGKKKKEE 600
           ELRG+R+GD + GGFNPKMFRSNSSVSWRS+SM+GG FSSSRKSNAESNGNG  KK++ +
Sbjct: 541 ELRGDRNGDVKAGGFNPKMFRSNSSVSWRSASMMGGSFSSSRKSNAESNGNGKKKKEEPQ 600

Query: 601 PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 640
           PVLERNRSAR+SPTNVDNGLLRFYLTP+RGSRRGGSGKVKP+QAQSIARSVLRLY
Sbjct: 601 PVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPSQAQSIARSVLRLY 632

BLAST of MC05g1525 vs. ExPASy TrEMBL
Match: A0A1S3B6I0 (UPF0503 protein At3g09070, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486324 PE=4 SV=1)

HSP 1 Score: 914 bits (2361), Expect = 0.0
Identity = 510/655 (77.86%), Postives = 559/655 (85.34%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS------S 60
           MNPSTD QP PPP LPPLPHRP+A CPRHP EHFT FCPLCLCERLSLLDSSS      S
Sbjct: 1   MNPSTDGQPLPPP-LPPLPHRPSATCPRHPQEHFTAFCPLCLCERLSLLDSSSAAAASSS 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRSTNRPS-FFPELRRTKSFSFSASRNDAF 120
           SSS++KP STAA+ALKAIFRP             NRPS FFPELRRTKSFS  AS+N+AF
Sbjct: 61  SSSSRKPHSTAASALKAIFRPPP----------PNRPSSFFPELRRTKSFS--ASKNEAF 120

Query: 121 SASFEPQRKSCDVRVRNTLCSLFSQEPSTSR--VAVAGAQISVESRNFEEANSGLGGPVL 180
           S  FEPQRKSCDVR+RNTLCSL SQ+ S+S   +A A  +I VE++N E+  S    P+ 
Sbjct: 121 STVFEPQRKSCDVRLRNTLCSLISQDASSSSKVLAPAAPEIVVETKNLEDPCSSWVEPIP 180

Query: 181 EVQEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESV--QQELKTMKDHIDLD 240
           +  +G+IRVS +PN  D VIEN VQEIV   EEEI+ ELE+ESV  Q+E KTMKDHIDLD
Sbjct: 181 D-GDGDIRVSGQPNVGDFVIENSVQEIV---EEEIQVELESESVHLQEEFKTMKDHIDLD 240

Query: 241 SNAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHF 300
           S+ KKPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHF
Sbjct: 241 SHTKKPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHF 300

Query: 301 RDTQSEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPT 360
           R+TQSEIADYGFGRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFP+MPT
Sbjct: 301 RETQSEIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPT 360

Query: 361 MLSVVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSDSS-RRRKSLDRSN 420
           MLSVVEDAPI+VFRSDTQIPVE+S NSTNE+EN+PGGSSQTR+YYSDSS RRRKSLDRSN
Sbjct: 361 MLSVVEDAPIHVFRSDTQIPVEDSINSTNEEENIPGGSSQTREYYSDSSSRRRKSLDRSN 420

Query: 421 SLRKTAAAVVAEIDEMKS-VSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDS 480
           S+RKTAAAVVAEID+MKS VSNAKVSPATTD+CH PKLG+PDRDSN+N LRDD SGS + 
Sbjct: 421 SIRKTAAAVVAEIDDMKSSVSNAKVSPATTDVCHVPKLGLPDRDSNSNSLRDDYSGSFED 480

Query: 481 GFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESWP 540
                 + NRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRES RPNGVERSYSESWP
Sbjct: 481 AASVVGTANRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWP 540

Query: 541 ELRGERSGDGR-GGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGKKKKEE 600
           ELRG+R+GD + GGFNPKMFRSNSSVSWRS+SM+GG FSSSRKSNAESNGNG  KK++ +
Sbjct: 541 ELRGDRNGDVKAGGFNPKMFRSNSSVSWRSASMMGGSFSSSRKSNAESNGNGKKKKEEPQ 600

Query: 601 PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 640
           PVLERNRSAR+SPTNVDNGLLRFYLTP+RGSRRGGSGKVKP+QAQSIARSVLRLY
Sbjct: 601 PVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPSQAQSIARSVLRLY 632

BLAST of MC05g1525 vs. ExPASy TrEMBL
Match: A0A0A0LAA9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G732510 PE=4 SV=1)

HSP 1 Score: 907 bits (2344), Expect = 0.0
Identity = 508/654 (77.68%), Postives = 561/654 (85.78%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS----SSS 60
           MNPSTD QP PPP LPPLPHRP+A CPRHP EHFT FCPLCLCERLSLLDSSS    SSS
Sbjct: 1   MNPSTDGQPLPPP-LPPLPHRPSATCPRHPQEHFTAFCPLCLCERLSLLDSSSAASSSSS 60

Query: 61  SAKKPPSTAAAALKAIFRPASAALPSTSNRSTNRPS-FFPELRRTKSFSFSASRNDAFS- 120
           S++KP STA++AL+A+FRP             NRPS FFPELRRTKSFS  AS+N+AFS 
Sbjct: 61  SSRKPHSTASSALRALFRPPP----------PNRPSSFFPELRRTKSFS--ASKNEAFST 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSR--VAVAGAQISVESRNFEEANSGLGGPVLE 180
           A FEPQRKSCDVR+RNTLCSL SQ+ S+S   +A A ++I+VE++N E+ +S    P+ +
Sbjct: 121 AIFEPQRKSCDVRLRNTLCSLISQDASSSSKLLAPAASEIAVETKNLEDPSSSWVEPIPD 180

Query: 181 VQEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQ--QELKTMKDHIDLDS 240
           V +G+IRVS +PN  D VIEN V+EIV   EEEI+ EL +ESVQ  +E KTMKDHIDLDS
Sbjct: 181 V-DGDIRVSGQPNVGDFVIENSVKEIV---EEEIQVELGSESVQLQEEFKTMKDHIDLDS 240

Query: 241 NAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFR 300
           + KKPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFR
Sbjct: 241 HTKKPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFR 300

Query: 301 DTQSEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPTM 360
           +TQSEIADYGFGRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFP+MPTM
Sbjct: 301 ETQSEIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTM 360

Query: 361 LSVVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSDSS-RRRKSLDRSNS 420
           LSVVEDAPI+VFRSDTQIPVE+S NSTNE+EN+PGGSSQTR+YYSDSS RRRKSLDRSNS
Sbjct: 361 LSVVEDAPIHVFRSDTQIPVEDSINSTNEEENIPGGSSQTREYYSDSSSRRRKSLDRSNS 420

Query: 421 LRKTAAAVVAEIDEMKS-VSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSG 480
           +RKTAAAVVAEID+MKS VSNAKVSPATTD+ HGPKLGIPDRDSN+N LRDD SGS +  
Sbjct: 421 IRKTAAAVVAEIDDMKSSVSNAKVSPATTDVSHGPKLGIPDRDSNSNSLRDDYSGSFEDA 480

Query: 481 FIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESWPE 540
                + NRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRES RPNGVERSYSESWPE
Sbjct: 481 ASVVGTANRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWPE 540

Query: 541 LRGERSGDGR-GGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGKKKKEEP 600
           LRG+R+GD + GGFNPKMFRSNSSVSWRS+SMIGG FSSSRKSNAESNGNG  KK++ +P
Sbjct: 541 LRGDRNGDVKAGGFNPKMFRSNSSVSWRSASMIGGSFSSSRKSNAESNGNGKKKKEEAQP 600

Query: 601 VLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 640
           VLERNRSAR SPTNVDNGLLRFYLTP+RGSRRG SGKVKP+QAQSIARSVLRLY
Sbjct: 601 VLERNRSARQSPTNVDNGLLRFYLTPLRGSRRGESGKVKPSQAQSIARSVLRLY 631

BLAST of MC05g1525 vs. TAIR 10
Match: AT3G09070.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 493.4 bits (1269), Expect = 2.7e-139
Identity = 359/729 (49.25%), Postives = 434/729 (59.53%), Query Frame = 0

Query: 1   MNPSTD-----AQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS-- 60
           MNP+TD     A    PPP PP PHR + +C RHP E FTGFCP CLCERLS+LD ++  
Sbjct: 1   MNPATDPVSAAAAALAPPPQPPQPHRLSTSCNRHPEERFTGFCPSCLCERLSVLDQTNNG 60

Query: 61  -SSSSAKKPPSTAAAALKAIFRPA---SAALPSTSNRSTNRPSFFPELRRTKSFSFSASR 120
            SSSS+KKPP+ +AAALKA+F+P+        +T+     +P FFPELRRTKSFS ++  
Sbjct: 61  GSSSSSKKPPTISAAALKALFKPSGNNGVGGVNTNGNGRVKPGFFPELRRTKSFS-ASKN 120

Query: 121 NDAFSASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVA-VAGAQISVESRNFEEANSGLGG 180
           N+ FS  FEPQR+SCDVR+R++L +LFSQ+   +  + V G +I VE R      S +  
Sbjct: 121 NEGFSGVFEPQRRSCDVRLRSSLWNLFSQDEQRNLPSNVTGGEIDVEPR-----KSSVAE 180

Query: 181 PVLEV-QEGEIRVSEEP----------NAVDVVIENGVQEIVEEGEEEI-EAELEAESV- 240
           PVLEV  EGE    +E            A D  I N   E++ E  +EI E   E E   
Sbjct: 181 PVLEVNDEGEAESDDEELEEEEEEDYVEAGDFEILNDSGELMREKSDEIVEVREEIEEAV 240

Query: 241 -------QQELKTMKDHIDLDSNAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEK 300
                  ++ELK +KD+IDLDS  KKPS      +  SFWSAASVFSKKLQKWR  QK K
Sbjct: 241 KPTKGLSEEELKPIKDYIDLDSQTKKPS------VRRSFWSAASVFSKKLQKWRQNQKMK 300

Query: 301 KQRNGS----GSTTLPVEKPIGRHFRDTQSEIADYGFGRRSCDTDPRFSLDA-------- 360
           K+RNG     GS  LPVEKPIGR  RDTQSEIADYG+GRRSCDTDPRFSLDA        
Sbjct: 301 KRRNGGDHRPGSARLPVEKPIGRQLRDTQSEIADYGYGRRSCDTDPRFSLDAGRFSLDAG 360

Query: 361 ------GRMSFDDPRYSFDEPRASWDGYLISRTF-------PKMPTMLSVVEDAP----I 420
                 GR+S DDPRYSFDEPRASWDG LI RT        P  P+MLSVVEDAP     
Sbjct: 361 RFSVDIGRISLDDPRYSFDEPRASWDGSLIGRTMFPPAARAPPPPSMLSVVEDAPPPVHR 420

Query: 421 NVFRSDTQIPVEE---------SPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDR-SN 480
           +V R+D Q PVEE           N  ++   +PGGS QTRDYY+D SSRRRKSLDR S+
Sbjct: 421 HVTRADMQFPVEEPAPPPPVVNQTNGVSDPVIIPGGSIQTRDYYTDSSSRRRKSLDRSSS 480

Query: 481 SLRKTAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDS 540
           S+RKTAAAVVA++DE K SVS+A     + D   G    + D ++ A    D+ S    +
Sbjct: 481 SMRKTAAAVVADMDEPKLSVSSA----ISIDAYSG---SLRDNNNYAVETADNGSFREPA 540

Query: 541 GFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNK-----DEEEDRESRPNG--VERS 600
             I     N  + +KKSRRWGK W+I GLI R+  NK     +EEEDR  R NG  VERS
Sbjct: 541 MMIGDRKVNSNDNNKKSRRWGK-WSILGLIYRKSVNKYEEEEEEEEDRYRRLNGGMVERS 600

Query: 601 YSESWPELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGK 641
            SESWPELR      G GG  P+M RSNS+VSWRSS                    G G 
Sbjct: 601 LSESWPELR----NGGGGGGGPRMVRSNSNVSWRSS--------------------GGGS 660

BLAST of MC05g1525 vs. TAIR 10
Match: AT2G38070.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 465.3 bits (1196), Expect = 7.9e-131
Identity = 324/659 (49.17%), Postives = 411/659 (62.37%), Query Frame = 0

Query: 4   STDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLD-----SSSSSSSA 63
           S  A    PPP PP PHRP+ +C RHP E FTGFCP CL +RLS+LD     +++ +SS+
Sbjct: 16  SASAVALAPPPHPPQPHRPSTSCDRHPDERFTGFCPSCLFDRLSVLDITGKNNNAVASSS 75

Query: 64  KKPPSTAAAALKAIFRPASAALPSTSNRSTNRPSFFPELRRTKSFSFSASRNDAFS-ASF 123
           KKPPS ++AALKAIF+P+S++            SFFPELRRTK  SFSAS+ +AFS  +F
Sbjct: 76  KKPPS-SSAALKAIFKPSSSS-----------GSFFPELRRTK--SFSASKAEAFSLGAF 135

Query: 124 EPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAGAQISVESRNFEEANSGLGGPVLEVQEGE 183
           EPQR+SCDVRVRNTL SLF ++   +     G  ++    + E  NS +  PV E +E E
Sbjct: 136 EPQRRSCDVRVRNTLWSLFHEDAEHNSQTKEGLSVNCSEIDLERINSIVKSPVFE-EETE 195

Query: 184 IRVSEEPNAVDVVIE------NGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSN 243
           I  SE+ N  D+  E      + + EIVEE EEE   ++E  +++   +T          
Sbjct: 196 IE-SEQDNEKDIKFETFKEPRSVIDEIVEEEEEEETKKVEDFTMEFNPQT---------- 255

Query: 244 AKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRN---GSGSTTLPVEKPIGRH 303
             K + RD KEIAGSFWSAASVFSKKLQKWR KQK KK R    G+GS+ LPVEK IGR 
Sbjct: 256 TAKKTNRDFKEIAGSFWSAASVFSKKLQKWRQKQKLKKHRTGNLGAGSSALPVEKAIGRQ 315

Query: 304 FRDTQSEIADYGFGRRSCDTDP-------RFSLDAGRMSFDDPRYSFDEPRASWDGYLIS 363
            RDTQSEIA+YG+GRRSCDTDP       RFSLDAGR+S DDPRYSF+EPRASWDGYLI 
Sbjct: 316 LRDTQSEIAEYGYGRRSCDTDPRFSIDAGRFSLDAGRVSVDDPRYSFEEPRASWDGYLIG 375

Query: 364 RTFP--KMPTMLSVVEDAPI--NVFRSDTQIPVEESPNSTNE--DENVPGGSSQTRDYY- 423
           R     +MP+MLSVVED+P+  +V RSDT IPVE+SP  +    DE VPGGS+QTR+YY 
Sbjct: 376 RAAAPMRMPSMLSVVEDSPVRNHVHRSDTHIPVEKSPQVSEAVIDEIVPGGSAQTREYYL 435

Query: 424 -SDSSRRRKSLDRSNSLRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDSN 483
            S SSRRRKSLDRS+S RK +A+V+AEIDE+K   + +                 D  S+
Sbjct: 436 DSSSSRRRKSLDRSSSTRKLSASVMAEIDELKLTQDREAK---------------DLVSH 495

Query: 484 ANPLRDDCSGSLDSGFIDAASGN------RKEESKKSRRWGKRWNIWGLINRRGGNKDEE 543
           +N LRDDC  S+++ +      N       K+ +KKS RW   WNI+GL++R+ GNK EE
Sbjct: 496 SNSLRDDCC-SVENNYEMGVRENVGTIECNKKRTKKS-RWS--WNIFGLLHRKNGNKYEE 555

Query: 544 EDRESRPNGVERSYSESWPELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSR 603
           E+R S   GV+R++S SW       + + R GF+PKM RSNSSVSWRSS   GG    + 
Sbjct: 556 EERRS---GVDRTFSGSW-------NVEPRNGFDPKMIRSNSSVSWRSSGTTGGGLQRN- 599

Query: 604 KSNAESNGNGNGKKKKEEPVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKP 627
                 +G  +GKKK                +  +NG+L+FYLTP +G RRG      P
Sbjct: 616 ----SVDGYISGKKK---------------VSKAENGMLKFYLTPGKGRRRGSGNSTAP 599

BLAST of MC05g1525 vs. TAIR 10
Match: AT5G01170.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 394.4 bits (1012), Expect = 1.7e-109
Identity = 320/684 (46.78%), Postives = 390/684 (57.02%), Query Frame = 0

Query: 1   MNPSTDAQP--APPPPLPP-LPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSSS--- 60
           MN S D  P  A     PP  PHR + +C  HP E F+GFCP CLC+RLS+LD +++   
Sbjct: 1   MNLSADQAPVTAVDELAPPSQPHRLSTSCDLHPEERFSGFCPSCLCDRLSVLDHNAAPPP 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRSTNRPSFFPELRRTKSFSFSASRNDAFS 120
           SSS++KPPS +A +LKA+F+P+S+   +++     RP FFPELRRTK  SFSA  N+ FS
Sbjct: 61  SSSSRKPPSISAVSLKALFKPSSSGTNNSNGNGRVRPGFFPELRRTK--SFSAKNNEGFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAGAQISVESRNFEEANSGLGGPVLEV- 180
             FEPQR+SCDVR+R+   +L   E +           SV+    E   S +   VLEV 
Sbjct: 121 GGFEPQRRSCDVRLRDDERNLPINEAA-----------SVDKIEEEARESSVSEIVLEVT 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
           +E EI   EE        E    EIVEE   EI  E      ++ELK MKD++DL S  K
Sbjct: 181 EEAEIEEDEENG------EKDPGEIVEEKSSEIGEE------EEELKPMKDYMDLYSQTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPS   +K+ AGSF+SAASVFSKKLQKW+ KQK KK RNG G          GR     Q
Sbjct: 241 KPS---VKDFAGSFFSAASVFSKKLQKWKQKQKVKKPRNGVGG---------GR----PQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDA-------GRMSFDDPRYSFDEPRASWDGYLISRT--- 360
           SEI   G GRRS DTDPRFSLDA       GR+S DD RYS DEPRASWDG+LI RT   
Sbjct: 301 SEI---GVGRRSSDTDPRFSLDAGRFSVDIGRISMDDSRYSLDEPRASWDGHLIGRTTAA 360

Query: 361 -FPKMPTMLSVVEDAPINVFRSDTQIPVEESPNSTNEDEN----VPGGSSQTRDYYS--D 420
             P  P+MLSVVE+AP+N  RSD QIP   S    + D +    +PGGS+QTRDYY+   
Sbjct: 361 RVPLPPSMLSVVENAPLN--RSDMQIPSSPSIKPISGDSDPIIIIPGGSNQTRDYYTGPP 420

Query: 421 SSRRRKSLDRSNSLRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDSNANP 480
           SSRRRKSLDRSNS+RK    +V E++++KSVSN     +TT I           DSN+  
Sbjct: 421 SSRRRKSLDRSNSIRK----IVTELEDVKSVSN-----STTTI-----------DSNSME 480

Query: 481 LRDDCSGSLDSGFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRESRPNG 540
             ++              GN+  + KKSRRWGK W+I G I R+ G  DEEEDR SR N 
Sbjct: 481 TAEN-------------KGNQNGD-KKSRRWGK-WSILGFIYRK-GKDDEEEDRYSRSNS 540

Query: 541 ---VERSYSESWPELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAES 600
              VERS SESWPE+R     +G GG  PKM RSNS+VSWRSS                 
Sbjct: 541 AGMVERSLSESWPEMR-----NGEGG-GPKMRRSNSNVSWRSS----------------- 568

Query: 601 NGNGNGKKKKEEPVLERNRSARYSPTNVDNGLLRFYLTPMR-------GSRRGGSG---- 641
            G G+           RN+S+RYS  + +NG+LRFYLTPMR       GS  GG G    
Sbjct: 601 -GGGSA----------RNKSSRYSSKDGENGMLRFYLTPMRRSWKTSGGSGGGGGGGGGG 568

BLAST of MC05g1525 vs. TAIR 10
Match: AT3G46990.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 180.6 bits (457), Expect = 3.9e-45
Identity = 201/650 (30.92%), Postives = 305/650 (46.92%), Query Frame = 0

Query: 21  RPAAACPRHPHEHFT-GFCPLCLCERLSLLDSSSSSSSAKKPPSTAAAALKAIFRPASAA 80
           R +++C RHP    T GFC  CL ERL  +++ SSS +A +                   
Sbjct: 11  RSSSSCHRHPSAKPTSGFCASCLRERLVTIEAQSSSLAAVQT------------------ 70

Query: 81  LPSTSNRSTNRPSFFPELRRTKSFSFSASRNDAFSASFEPQRKSCDVR-VRNTLCSLFSQ 140
                          PELRR +S+S    RN + S S +P+R+SCDVR   ++L  LF  
Sbjct: 71  ---------------PELRRIRSYSV---RNASVSVSDQPRRRSCDVRSSASSLLDLFVD 130

Query: 141 EPSTSRVAVAGAQISVESRNFEEANSGLGGPVL-EVQEGEIRVSEEPNAVDVVIENGVQE 200
           +                    E  +S +  P++ +++E E    EE +  D         
Sbjct: 131 DDE------------------ERVDSSIRKPLVPDLKEEEEEEEEEEDYYD--------- 190

Query: 201 IVEEGEEEIEA--ELEAESVQQELKTMKDHIDLD--SNAKKPSGRDLKEIAGSFWSAASV 260
                 E+I+   E +   + +E KTMK+ IDLD  +  KK +G+DLKEI       ASV
Sbjct: 191 -----GEDIKGFDEGKPRKIVEENKTMKEFIDLDWRNQIKKNNGKDLKEI-------ASV 250

Query: 261 FSKKLQKWR-DKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQSEIADYGFGRRSCDTDPRF 320
            S++L+ +  +K+ ++K                      + S  A    GR S D DPR 
Sbjct: 251 LSRRLKNFTLNKRNDEK----------------------SDSRFAGIVNGRHSSDVDPRL 310

Query: 321 SLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPKMPTMLSVVEDAPINVFRSDTQIPVEE 380
           S D GR+       SF++PR+SWDG LI +++ K+ T+ +V EDA         +  VEE
Sbjct: 311 SFDGGRI-------SFEKPRSSWDGCLIEKSYHKLTTLSTVTEDA-------KAKCGVEE 370

Query: 381 SPNSTNEDENVPGGSSQTRDYYSDSSRRRKSLDRSNSLRKTAAAVVAEIDEMKSVSNAKV 440
                 E E  PGG+ QT++YYSD SRRR+S DRS S+++     + E+DE++ +SNAKV
Sbjct: 371 --EEVEEKEKSPGGTVQTKNYYSD-SRRRRSFDRSVSIKRQG---LLEVDELRGISNAKV 430

Query: 441 SPATTDICHGPKLGIPD---RDSN---ANPLRDDCSGSLDSGFIDAASG--NRKEES--- 500
           SP T  + HG KL + +   RDSN      ++ +    +  G I  A+G   +K++S   
Sbjct: 431 SPETVGLFHGAKLLVTEKELRDSNWYSIKNVKPESKELVSKGKICIAAGGEGKKQDSVEL 490

Query: 501 -KKSRRWGKRWNIWGLINRRGGNKDE---EEDRESRPNGVERSYSESWPELRGERSGDGR 560
            K  ++W K WNIWGLI R+   K+E   E+  +   N VE S +ES  +LR    G+  
Sbjct: 491 KKPRKKWPKGWNIWGLIQRKNEAKNEIKTEQILKLEGNAVEGSLAESLLKLRRVGKGETN 542

Query: 561 GGFNPKMFRSNSSVSWRSS----------------------SMIGGPFSSSRKSNAESNG 620
            G + K+ +S  SVS R S                       +  G  +S        +G
Sbjct: 551 VGVSEKLLKS-YSVSARKSCDGVRSGANIVSGFEGGRSSCDGLFHGSINSVEAGRNSCDG 542

Query: 621 NGNG-KKKKEEPVLERNRS-ARYSPTNVDNGLLRFYLTPMRGSRRGGSGK 624
             NG + K+   +L+RN +    S  N++  + RFYL+P++  +   SGK
Sbjct: 611 LVNGIEGKQNHHLLQRNANVGTCSQENLEKSMFRFYLSPVKSHKTSKSGK 542

BLAST of MC05g1525 vs. TAIR 10
Match: AT5G58930.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 178.3 bits (451), Expect = 1.9e-44
Identity = 208/648 (32.10%), Postives = 294/648 (45.37%), Query Frame = 0

Query: 21  RPAAACPRHP-HEHFTGFCPLCLCERLSLLDSSSSSSSAKKPPSTAAAALKAIFRPASAA 80
           R +A C RHP  +  TGFC  CL ERLS +++ SSS SA                     
Sbjct: 12  RHSAVCHRHPSSKPTTGFCATCLRERLSTIEALSSSVSAS-------------------- 71

Query: 81  LPSTSNRSTNRPSFFPELRRTKSFSFSASRNDAFSASFEPQRKSCDVRVRNTLCSLFSQE 140
                           ELRR +S+S    R+ + S   +P+R+SCDVR            
Sbjct: 72  ---------------TELRRVRSYSV---RDASASVLDQPRRRSCDVR------------ 131

Query: 141 PSTSRVAVAGAQISVESRNFEEANSGLGGPVLEVQEGEIRVSEEPNAVDVVIENGVQEIV 200
                             N ++ +        E+ +  IR    P+ ++       +E  
Sbjct: 132 -----------------SNHDDDDDD------ELLKSSIRFPIVPDLIE-----DEEEED 191

Query: 201 EEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK--KPSGRDLKEIAGSFWSAASVFSKK 260
           +EG++ +E E+E      E KTMK+ IDL+S  +  K +G+D            SVFS+ 
Sbjct: 192 DEGKKLVEEEIE----DGEQKTMKELIDLESRNQQLKNNGKD------------SVFSRT 251

Query: 261 LQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQSEIADYGFGRRSCDTDPRFSLDAG 320
           L+K+  K   K   +G+                           GRRSCD DPR SLDAG
Sbjct: 252 LRKFSLKHHRKIPDSGN-------------------------SLGRRSCDVDPRLSLDAG 311

Query: 321 RMSFDDPRYSFDEPRASWDGYLISRTFPKMPTMLSVVEDAPINVFRSDTQIPVEESPNST 380
           R+       SFDEPRASWDG LI +T+PK+  + SV ED   +        P + +    
Sbjct: 312 RV-------SFDEPRASWDGCLIGKTYPKLIPLSSVTEDVKAS--------PEKITGEKV 371

Query: 381 NEDE-NVPGGSSQTRDYYSDSSRRRKSLDRSNSLRKTAAAVVAEIDEMKSVSNAKVSPAT 440
            EDE N PGG++QTRDYY D SRRR+S DRS      +   + E+DE+K++SNAKVSP T
Sbjct: 372 EEDEKNNPGGTAQTRDYYLD-SRRRRSFDRS------SRHGLLEVDELKAISNAKVSPET 431

Query: 441 TDICHGPKLGIPD---RDSNANPLRDDCSGSLD---SGFIDAASGNRKEE-----SKKSR 500
             + HG KL + +   RDSN   +++    SL+    G    A+G  K++      K  +
Sbjct: 432 VGLFHGAKLLVTERELRDSNWYSIKNYKPESLELGSKGVGCVAAGEVKKQDGFGLKKSGK 491

Query: 501 RWGKRWNIWGLINRRGG-NKDEEEDRESRP---NGVERSYSESWPELRGERSGDGRGGFN 560
            W K WN WGLI R+    K+E +  +S     N +E S +ES  +LR    G+  G  +
Sbjct: 492 NWSKGWNFWGLIQRKTDVAKNEMKTEQSLKLGGNTMEGSLAESLLKLRRVAKGETNGDVS 516

Query: 561 PKMFRSNSSVSWRSS------------------SMIGGPFSSS-------RKSNAESNGN 620
            K+ RS  SVS R S                  S   G F  S       R+S  E +G 
Sbjct: 552 EKLIRS-YSVSARKSCDGMLRGASIVNGFEGGRSSCDGLFHGSITGVETGRRSLCE-DGM 516

Query: 621 GNGKKKKEEPVLER-NRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGK 624
            +G + K   +L+  ++   YSP N+ NG++RFYLTP+       SGK
Sbjct: 612 FHGVEGKRNHLLQSDDKLGTYSPDNLRNGMVRFYLTPLNSHMTSKSGK 516