MC05g1521 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g1521
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Steroid 5-alpha-reductase DET2
Location	MC05: 19043174 .. 19043947 (-)
RNA-Seq Expression	MC05g1521
Synteny	MC05g1521

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCACTCATGGGTTCGGATCAGGCTCTGTTCCGCAACAGCCTCCTCGCCCTGTACCTCATCGGACCGCCGACCGCCGTCGCCCTGAGGTTCCTTCAGGCGCCGTACGGGAAGCACAACCGGCCGGGGTGGGGGCCGACTATGTCCGCGCCCTTGGCCTGGTTCCTGATGGAGAGCCCCACCCTGTGGCTCACCCTCCTCCTCTTCCCCTTCGGCGATCACTCCTCCGAGCCCAAGCCGATCCTTCTCATCTCCCCCTTCCTCCTCCACTACCTCCATCGCACCTGCCTCTACCCAATTCGGCTTCTCCGGCAACGGAATTCCGCCGCGGGTTTCCCGGTGGCGGTGGCGCTCATGGCATTCGGGTTCAATGTGCTGAATTCTTACCTGCAAACCAGATCGGTGTCTCACTACAAGAGCTACGAGGGAGACGGGTGGTTTTGGTGGCGGTTTTCCGTTGGGGCGGTGGTTTTTGCGACGGGGATGGGAGTGAATATTTGGTCGGACGGTGTTTTGGTAGGACTGAAGAGGGCCGGCGGCGGGTATAAACTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGCCCTAATTACTTGGGGGAGGTGGTGGAGTGGCTGGGCTGGGCCGTGATGTGCTGGTCCTGGGCCGGGCTTGGGTTTTTCCTTTACACGTGTGCCAACTTGGTGCCTAGGGCACGTGCGAACCACACGTGGTATCTGAACAAGTTTGGGGAGGATTATCCCACGACTAGGAAGGCCATTATTCCCTTCTTGTAT

mRNA sequence

TCACTCATGGGTTCGGATCAGGCTCTGTTCCGCAACAGCCTCCTCGCCCTGTACCTCATCGGACCGCCGACCGCCGTCGCCCTGAGGTTCCTTCAGGCGCCGTACGGGAAGCACAACCGGCCGGGGTGGGGGCCGACTATGTCCGCGCCCTTGGCCTGGTTCCTGATGGAGAGCCCCACCCTGTGGCTCACCCTCCTCCTCTTCCCCTTCGGCGATCACTCCTCCGAGCCCAAGCCGATCCTTCTCATCTCCCCCTTCCTCCTCCACTACCTCCATCGCACCTGCCTCTACCCAATTCGGCTTCTCCGGCAACGGAATTCCGCCGCGGGTTTCCCGGTGGCGGTGGCGCTCATGGCATTCGGGTTCAATGTGCTGAATTCTTACCTGCAAACCAGATCGGTGTCTCACTACAAGAGCTACGAGGGAGACGGGTGGTTTTGGTGGCGGTTTTCCGTTGGGGCGGTGGTTTTTGCGACGGGGATGGGAGTGAATATTTGGTCGGACGGTGTTTTGGTAGGACTGAAGAGGGCCGGCGGCGGGTATAAACTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGCCCTAATTACTTGGGGGAGGTGGTGGAGTGGCTGGGCTGGGCCGTGATGTGCTGGTCCTGGGCCGGGCTTGGGTTTTTCCTTTACACGTGTGCCAACTTGGTGCCTAGGGCACGTGCGAACCACACGTGGTATCTGAACAAGTTTGGGGAGGATTATCCCACGACTAGGAAGGCCATTATTCCCTTCTTGTAT

Coding sequence (CDS)

TCACTCATGGGTTCGGATCAGGCTCTGTTCCGCAACAGCCTCCTCGCCCTGTACCTCATCGGACCGCCGACCGCCGTCGCCCTGAGGTTCCTTCAGGCGCCGTACGGGAAGCACAACCGGCCGGGGTGGGGGCCGACTATGTCCGCGCCCTTGGCCTGGTTCCTGATGGAGAGCCCCACCCTGTGGCTCACCCTCCTCCTCTTCCCCTTCGGCGATCACTCCTCCGAGCCCAAGCCGATCCTTCTCATCTCCCCCTTCCTCCTCCACTACCTCCATCGCACCTGCCTCTACCCAATTCGGCTTCTCCGGCAACGGAATTCCGCCGCGGGTTTCCCGGTGGCGGTGGCGCTCATGGCATTCGGGTTCAATGTGCTGAATTCTTACCTGCAAACCAGATCGGTGTCTCACTACAAGAGCTACGAGGGAGACGGGTGGTTTTGGTGGCGGTTTTCCGTTGGGGCGGTGGTTTTTGCGACGGGGATGGGAGTGAATATTTGGTCGGACGGTGTTTTGGTAGGACTGAAGAGGGCCGGCGGCGGGTATAAACTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGCCCTAATTACTTGGGGGAGGTGGTGGAGTGGCTGGGCTGGGCCGTGATGTGCTGGTCCTGGGCCGGGCTTGGGTTTTTCCTTTACACGTGTGCCAACTTGGTGCCTAGGGCACGTGCGAACCACACGTGGTATCTGAACAAGTTTGGGGAGGATTATCCCACGACTAGGAAGGCCATTATTCCCTTCTTGTAT

Protein sequence

SLMGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLWLTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGGGYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPTTRKAIIPFLY

Homology

[Close]

BLAST of MC05g1521 vs. ExPASy Swiss-Prot
Match: Q38944 (Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2)

HSP 1 Score: 375.6 bits (963), Expect = 4.7e-103
Identity = 171/258 (66.28%), Postives = 200/258 (77.52%), Query Frame = 0


Query: 5   SDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLWLT 64
           +D+  FR  LL L   GPPTAV L+FLQAPYGKHNR GWGPT+S P+AWF+MESPTLWLT
Sbjct: 5   ADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLT 64

Query: 65  LLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAA--GFPVAVALMAFGF 124
           LLLFPFG H+  PK +LL SP+L+HY HRT +YP+RL R    A   GFP+ +A +AF F
Sbjct: 65  LLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNGFPITIAALAFTF 124

Query: 125 NVLNSYLQTRSVSHYK-SYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAG-GG 184
           N+LN Y+Q R VSHYK  YE   WFWWRF +G VVF TGM +NI SD  LV LK+   GG
Sbjct: 125 NLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENRGG 184

Query: 185 YKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLN 244
           Y +P GGWFELVSCPNY GE +EWLGWAVM WSWAG+GFFLYTC+NL PRARA+H WY+ 
Sbjct: 185 YVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWYIA 244

Query: 245 KFGEDYPTTRKAIIPFLY 259
           KF E+YP TRKA+IPF+Y
Sbjct: 245 KFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of MC05g1521 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.4e-102
Identity = 174/258 (67.44%), Postives = 195/258 (75.58%), Query Frame = 0


Query: 3   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 62
           M SDQ LF   LL LY+I  PT ++L FLQAPYGKHNRPGWGPT+S PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLW 60

Query: 63  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGF 122
           LT  LFP G H   PK  LLISPFL HY +RT LYP+RL R      GFPV+VA MAFGF
Sbjct: 61  LTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRLARNTTQTRGFPVSVAFMAFGF 120

Query: 123 NVLNSYLQTRSVSHYK-SYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAG-GG 182
           N+LN YLQ R VSHYK  YE +  FWWRF  G ++F  GM VN+ +D VLVGLK+ G GG
Sbjct: 121 NLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQGDGG 180

Query: 183 YKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLN 242
           YK+P GG FELVSCPNY GE++EW GWAVM WSW G GFFLYTCANL+PRARA   WYL 
Sbjct: 181 YKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLWYLE 240

Query: 243 KFGEDYPTTRKAIIPFLY 259
           KF +DYP  RKA+IPF+Y
Sbjct: 241 KFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of MC05g1521 vs. ExPASy Swiss-Prot
Match: Q5K2N1 (Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 8.0e-87
Identity = 146/258 (56.59%), Postives = 190/258 (73.64%), Query Frame = 0


Query: 2   LMGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKH-NRPGWGPTMSAPLAWFLMESPT 61
           +  SD+ LF   +  + ++  PT +  +F  +PYGKH      G T+S P+AW  MESPT
Sbjct: 1   MFSSDENLFNFIVFFILVMAFPTFILCQFFTSPYGKHYTSADSGTTISPPIAWAFMESPT 60

Query: 62  LWLTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAF 121
           LWLT+++F  G + + P   LLISP+L HY +RT +YP+R LR RN+   FP+ +A+ AF
Sbjct: 61  LWLTIIVFRLGKNYTNPLAFLLISPYLFHYTNRTIIYPLR-LRSRNTKNNFPLNIAVTAF 120

Query: 122 GFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGGG 181
            FN+LN+Y+Q+R VSHY +Y+ D WFW RF +G V+F +GM +NIW+DGVL+GLK  GGG
Sbjct: 121 IFNLLNAYIQSRWVSHYANYQEDDWFWVRFGIGLVIFGSGMLLNIWADGVLLGLKSQGGG 180

Query: 182 YKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLN 241
           YK+P GG F+ VS PNYLGE++EWLGWA+M WSWAGL FF+YTCANLVPRA +NH WYL 
Sbjct: 181 YKIPRGGLFDYVSSPNYLGEIMEWLGWALMTWSWAGLAFFVYTCANLVPRAVSNHKWYLQ 240

Query: 242 KFGEDYPTTRKAIIPFLY 259
           KFGEDYP  RKA+ PFLY
Sbjct: 241 KFGEDYPKNRKAVFPFLY 257

[Close]

BLAST of MC05g1521 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.9e-36
Identity = 89/232 (38.36%), Postives = 125/232 (53.88%), Query Frame = 0


Query: 35  YGKHNRPGWGPTMSAPLAWFLMESPTLWLTLLLFPFGDHSSEPKP-------ILLISPFL 94
           YG+H  P +   + A  AW + E P+L L     P   ++SE  P        +L++ FL
Sbjct: 42  YGRHASPSFRVRVPARAAWVVQELPSLAL-----PLYQYASESAPRLRSAPNCILLAMFL 101

Query: 95  LHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGFNVLNSYLQTRSVSHYKSYEGDGWFW 154
           +HY HR  +YP  +          P+    MA  F   N YLQ+R +SH+  Y  D    
Sbjct: 102 VHYGHRCLIYPFLM----RGGKPMPLLACTMAIMFCTFNGYLQSRYLSHWAVYADDWVTD 161

Query: 155 WRFSVGAVVFATGMGVNIWSDGVLVGLKRAGG-GYKLPTGGWFELVSCPNYLGEVVEWLG 214
            RF +G  ++  GM +NI SD +L  L++ G  GYK+P GG FE V+  NY GE++EW G
Sbjct: 162 PRFLIGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCG 221

Query: 215 WAVMCWSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPTTRKAIIPFLY 259
           +A+  WS  G  F  +T   L  RA+ +H WYL KF E+YP  RK +IPFL+
Sbjct: 222 YALASWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKF-EEYPKFRKILIPFLF 263

[Close]

BLAST of MC05g1521 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 152.9 bits (385), Expect = 4.9e-36
Identity = 95/235 (40.43%), Postives = 133/235 (56.60%), Query Frame = 0


Query: 26  VALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTL-W-LTLLLFPFGDHSSEPKPILLI 85
           V L+ + + YG+++    G  + A  AWFL E P++ W L   + P           +L+
Sbjct: 25  VGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLL 84

Query: 86  SPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGFNVLNSYLQTRSVSHYKSYEGD 145
           + FL+HY+ RT ++P+ L+R       F     ++AF F  LN YLQ+R +S +  Y  D
Sbjct: 85  AMFLIHYVQRTLVFPV-LIRGGKPTLLF---TFVLAFLFCTLNGYLQSRYLSQFAVYAED 144

Query: 146 GWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAG-GGYKLPTGGWFELVSCPNYLGEVV 205
                 F  G  ++  GM +NI SD +L  L++ G  GYK+P GG FE VS  NY GE+V
Sbjct: 145 WVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANYFGELV 204

Query: 206 EWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPTTRKAIIPFL 258
           EW G+A+  WS  G+ F L+T   L  RAR +H WYL KF EDYP TRK +IPFL
Sbjct: 205 EWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKF-EDYPKTRKILIPFL 254

[Close]

BLAST of MC05g1521 vs. NCBI nr
Match: XP_022145671.1 (steroid 5-alpha-reductase DET2 [Momordica charantia])

HSP 1 Score: 546 bits (1407), Expect = 1.70e-196
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 3   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 62
           MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60

Query: 63  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGF 122
           LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGF
Sbjct: 61  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGF 120

Query: 123 NVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGGGYK 182
           NVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGGGYK
Sbjct: 121 NVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGGGYK 180

Query: 183 LPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLNKF 242
           LPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLNKF
Sbjct: 181 LPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLNKF 240

Query: 243 GEDYPTTRKAIIPFLY 258
           GEDYPTTRKAIIPFLY
Sbjct: 241 GEDYPTTRKAIIPFLY 256

[Close]

BLAST of MC05g1521 vs. NCBI nr
Match: XP_038905208.1 (LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida])

HSP 1 Score: 470 bits (1209), Expect = 2.92e-166
Identity = 221/259 (85.33%), Postives = 231/259 (89.19%), Query Frame = 0


Query: 3   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 62
           MGSDQALF NSLL LYLIGP   VALRFLQAPYGKHN+PGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGSDQALFHNSLLRLYLIGPRQVVALRFLQAPYGKHNQPGWGPTMSAPLAWFLMESPTLW 60

Query: 63  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLR---QRNSAAGFPVAVALMA 122
           LTLLLFP GDHSS  K +LLISPFLLHY +RTCLYPIRLLR   +RN+AAGFPV VA+MA
Sbjct: 61  LTLLLFPSGDHSSNSKALLLISPFLLHYFNRTCLYPIRLLRSGTRRNTAAGFPVIVAVMA 120

Query: 123 FGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGG 182
           FGFN+LNSYLQTRSVSHY  YEGDGWFWWRF  G VVF  GM VNIWSDGVLVGLKR GG
Sbjct: 121 FGFNLLNSYLQTRSVSHYMEYEGDGWFWWRFFGGGVVFLLGMSVNIWSDGVLVGLKRGGG 180

Query: 183 GYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYL 242
           GYK+PTG WFELVSCPNYLGE+VEWLGWAVMC SWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGWWFELVSCPNYLGEIVEWLGWAVMCGSWAGLAFFLYTCANLVPRARANHTWYL 240

Query: 243 NKFGEDYPTTRKAIIPFLY 258
           NKFGEDYP  RKA+IPFLY
Sbjct: 241 NKFGEDYPRNRKAVIPFLY 259

[Close]

BLAST of MC05g1521 vs. NCBI nr
Match: KAA0044147.1 (steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa])

HSP 1 Score: 469 bits (1208), Expect = 4.14e-166
Identity = 218/259 (84.17%), Postives = 233/259 (89.96%), Query Frame = 0


Query: 3   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 62
           MG DQALFRNSLLALYLIGPPT V+LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 63  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLL---RQRNSAAGFPVAVALMA 122
           LTLLLFP GDHSS+ K +LLISPFLLHYL+RTC YPIRLL    +RN+ AGFPV VA+MA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 123 FGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGG 182
           FGFN+LN+Y+QTRSVSHY  YEGDGWFWWRF  G VVF  GM VNIWSDGVLVGLKR+GG
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 180

Query: 183 GYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYL 242
           GYK+P GGWFELVSCPNYLGE++EWLGW VMC SWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 243 NKFGEDYPTTRKAIIPFLY 258
           NKFGEDYP  RKA+IPFLY
Sbjct: 241 NKFGEDYPINRKAVIPFLY 259

[Close]

BLAST of MC05g1521 vs. NCBI nr
Match: XP_008442471.1 (PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo])

HSP 1 Score: 470 bits (1209), Expect = 9.32e-166
Identity = 218/261 (83.52%), Postives = 235/261 (90.04%), Query Frame = 0


Query: 1   SLMGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPT 60
           ++MG DQALFRNSLLALYLIGPPT V+LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPT
Sbjct: 31  TVMGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPT 90

Query: 61  LWLTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLL---RQRNSAAGFPVAVAL 120
           LWLTLLLFP GDHSS+ K +LLISPFLLHYL+RTC YPIRLL    +RN+ AGFPV VA+
Sbjct: 91  LWLTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAI 150

Query: 121 MAFGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRA 180
           MAFGFN+LN+Y+QTRSVSHY  YEGDGWFWWRF  G VVF  GM VNIWSDGVLVGLKR+
Sbjct: 151 MAFGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRS 210

Query: 181 GGGYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTW 240
           GGGYK+P GGWFELVSCPNYLGE++EWLGW VMC SWAGLGFFLYTCANLVPRARANHTW
Sbjct: 211 GGGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTW 270

Query: 241 YLNKFGEDYPTTRKAIIPFLY 258
           YLNKFGEDYP  RKA+IPFLY
Sbjct: 271 YLNKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of MC05g1521 vs. NCBI nr
Match: XP_004137737.1 (steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protein Csa_002591 [Cucumis sativus])

HSP 1 Score: 466 bits (1200), Expect = 2.19e-164
Identity = 218/261 (83.52%), Postives = 232/261 (88.89%), Query Frame = 0


Query: 1   SLMGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPT 60
           ++MG DQALF NSLLALYLIGPPT V+LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPT
Sbjct: 31  TMMGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPT 90

Query: 61  LWLTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLL---RQRNSAAGFPVAVAL 120
           LWLTLLLFP GDHSS  K +LLISPFLLHYL+RTC YPIRLL    +RN+  GFPV VA+
Sbjct: 91  LWLTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNAQVGFPVLVAI 150

Query: 121 MAFGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRA 180
           MAFGFN+LNSY+QTRSVSHY  YEGDGWFWWRF  G VVF  GM VNIWSDGVLVGLKR 
Sbjct: 151 MAFGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRR 210

Query: 181 GGGYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTW 240
           GGGYK+PTGGWFELVSCPNYLGE++EWLGWAVMC SWAGLGFFLYTCANLVPRARANHTW
Sbjct: 211 GGGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTW 270

Query: 241 YLNKFGEDYPTTRKAIIPFLY 258
           YLNKFGE YP  RKA+IPFLY
Sbjct: 271 YLNKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of MC05g1521 vs. ExPASy TrEMBL
Match: A0A6J1CX04 (Steroid 5-alpha-reductase DET2 OS=Momordica charantia OX=3673 GN=LOC111015065 PE=3 SV=1)

HSP 1 Score: 546 bits (1407), Expect = 8.23e-197
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 3   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 62
           MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60

Query: 63  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGF 122
           LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGF
Sbjct: 61  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGF 120

Query: 123 NVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGGGYK 182
           NVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGGGYK
Sbjct: 121 NVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGGGYK 180

Query: 183 LPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLNKF 242
           LPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLNKF
Sbjct: 181 LPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLNKF 240

Query: 243 GEDYPTTRKAIIPFLY 258
           GEDYPTTRKAIIPFLY
Sbjct: 241 GEDYPTTRKAIIPFLY 256

[Close]

BLAST of MC05g1521 vs. ExPASy TrEMBL
Match: A0A5D3DNY2 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001080 PE=3 SV=1)

HSP 1 Score: 469 bits (1208), Expect = 2.01e-166
Identity = 218/259 (84.17%), Postives = 233/259 (89.96%), Query Frame = 0


Query: 3   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 62
           MG DQALFRNSLLALYLIGPPT V+LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 63  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLL---RQRNSAAGFPVAVALMA 122
           LTLLLFP GDHSS+ K +LLISPFLLHYL+RTC YPIRLL    +RN+ AGFPV VA+MA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 123 FGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGG 182
           FGFN+LN+Y+QTRSVSHY  YEGDGWFWWRF  G VVF  GM VNIWSDGVLVGLKR+GG
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 180

Query: 183 GYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYL 242
           GYK+P GGWFELVSCPNYLGE++EWLGW VMC SWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 243 NKFGEDYPTTRKAIIPFLY 258
           NKFGEDYP  RKA+IPFLY
Sbjct: 241 NKFGEDYPINRKAVIPFLY 259

[Close]

BLAST of MC05g1521 vs. ExPASy TrEMBL
Match: A0A1S3B6E3 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1)

HSP 1 Score: 470 bits (1209), Expect = 4.51e-166
Identity = 218/261 (83.52%), Postives = 235/261 (90.04%), Query Frame = 0


Query: 1   SLMGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPT 60
           ++MG DQALFRNSLLALYLIGPPT V+LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPT
Sbjct: 31  TVMGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPT 90

Query: 61  LWLTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLL---RQRNSAAGFPVAVAL 120
           LWLTLLLFP GDHSS+ K +LLISPFLLHYL+RTC YPIRLL    +RN+ AGFPV VA+
Sbjct: 91  LWLTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAI 150

Query: 121 MAFGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRA 180
           MAFGFN+LN+Y+QTRSVSHY  YEGDGWFWWRF  G VVF  GM VNIWSDGVLVGLKR+
Sbjct: 151 MAFGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRS 210

Query: 181 GGGYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTW 240
           GGGYK+P GGWFELVSCPNYLGE++EWLGW VMC SWAGLGFFLYTCANLVPRARANHTW
Sbjct: 211 GGGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTW 270

Query: 241 YLNKFGEDYPTTRKAIIPFLY 258
           YLNKFGEDYP  RKA+IPFLY
Sbjct: 271 YLNKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of MC05g1521 vs. ExPASy TrEMBL
Match: A0A0A0LAB3 (Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 SV=1)

HSP 1 Score: 466 bits (1200), Expect = 1.06e-164
Identity = 218/261 (83.52%), Postives = 232/261 (88.89%), Query Frame = 0


Query: 1   SLMGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPT 60
           ++MG DQALF NSLLALYLIGPPT V+LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPT
Sbjct: 31  TMMGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPT 90

Query: 61  LWLTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLL---RQRNSAAGFPVAVAL 120
           LWLTLLLFP GDHSS  K +LLISPFLLHYL+RTC YPIRLL    +RN+  GFPV VA+
Sbjct: 91  LWLTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNAQVGFPVLVAI 150

Query: 121 MAFGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRA 180
           MAFGFN+LNSY+QTRSVSHY  YEGDGWFWWRF  G VVF  GM VNIWSDGVLVGLKR 
Sbjct: 151 MAFGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRR 210

Query: 181 GGGYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTW 240
           GGGYK+PTGGWFELVSCPNYLGE++EWLGWAVMC SWAGLGFFLYTCANLVPRARANHTW
Sbjct: 211 GGGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTW 270

Query: 241 YLNKFGEDYPTTRKAIIPFLY 258
           YLNKFGE YP  RKA+IPFLY
Sbjct: 271 YLNKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of MC05g1521 vs. ExPASy TrEMBL
Match: A0A6J1J7F2 (Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 SV=1)

HSP 1 Score: 461 bits (1187), Expect = 3.18e-163
Identity = 219/259 (84.56%), Postives = 230/259 (88.80%), Query Frame = 0


Query: 3   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 62
           M SDQ LF NSLLALYLIGPPTA++LRFLQAPYGKHNRPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAISLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 63  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLR---QRNSAAGFPVAVALMA 122
           LTLLLFP GD+SS  K +LLIS FLLHYL+RTCLYPIRLLR   +RNSA GFPV VALMA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 123 FGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGG 182
           F FN+LNSYLQTRSVSHY  Y+ D WFWWRF  GAVVF  GM VNIWSDGVLV LK AGG
Sbjct: 121 FVFNLLNSYLQTRSVSHYMDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGG 180

Query: 183 GYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYL 242
           GYK+PTGGWFELVSCPNYLGE+VEWLGWAVMC SWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 243 NKFGEDYPTTRKAIIPFLY 258
           NKFGEDYP  RKA+IPFLY
Sbjct: 241 NKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of MC05g1521 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 375.6 bits (963), Expect = 3.3e-104
Identity = 171/258 (66.28%), Postives = 200/258 (77.52%), Query Frame = 0


Query: 5   SDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLWLT 64
           +D+  FR  LL L   GPPTAV L+FLQAPYGKHNR GWGPT+S P+AWF+MESPTLWLT
Sbjct: 5   ADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLT 64

Query: 65  LLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAA--GFPVAVALMAFGF 124
           LLLFPFG H+  PK +LL SP+L+HY HRT +YP+RL R    A   GFP+ +A +AF F
Sbjct: 65  LLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNGFPITIAALAFTF 124

Query: 125 NVLNSYLQTRSVSHYK-SYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAG-GG 184
           N+LN Y+Q R VSHYK  YE   WFWWRF +G VVF TGM +NI SD  LV LK+   GG
Sbjct: 125 NLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENRGG 184

Query: 185 YKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLN 244
           Y +P GGWFELVSCPNY GE +EWLGWAVM WSWAG+GFFLYTC+NL PRARA+H WY+ 
Sbjct: 185 YVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWYIA 244

Query: 245 KFGEDYPTTRKAIIPFLY 259
           KF E+YP TRKA+IPF+Y
Sbjct: 245 KFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of MC05g1521 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 72.4 bits (176), Expect = 6.0e-13
Identity = 53/190 (27.89%), Postives = 94/190 (49.47%), Query Frame = 0


Query: 74  SSEPKPILLISPFLLHYLHRTCLYPIRLLRQRNSAAGFPVAVALMAFGFNVLNSYLQTRS 133
           S + + +LL S   LH+  R       +L     + G  +  AL      + +SY  + +
Sbjct: 89  SDDLRFLLLKSALALHFFKRV----FEVLFIHKYSGGMAIDSAL-----TISSSYFSSTA 148

Query: 134 VSHYKSYEGDGWFWWRFSV---GAVVFATGMGVNIWSDGVLVGLKRAGG--GYKLPTGGW 193
           +  Y      G     F +   G V+F  G+  N++   +L  L++  G   YK+P GG 
Sbjct: 149 LMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGL 208

Query: 194 FELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPT 253
           F+++ CP+YL E++ +  + ++  +     F + T   L+ R+ A  TWYL+KF +D+P 
Sbjct: 209 FDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGRSYATRTWYLSKF-DDFPK 268

Query: 254 TRKAIIPFLY 259
             KA+IPF++
Sbjct: 269 HIKALIPFVF 268

[Close]

BLAST of MC05g1521 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 68.9 bits (167), Expect = 6.6e-12
Identity = 36/83 (43.37%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 181 YKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWS-----WAGLGFFLYTCANLVPRARANH 240
           Y +P G WFE+VSCP++L E+V +LG  +         W   GF     ANL   A   H
Sbjct: 242 YIIPYGDWFEMVSCPHFLAEIVLYLGLLISSGGTDISIWLLFGF---VAANLTYAAGETH 301

Query: 241 TWYLNKFGEDYPTTRKAIIPFLY 259
            WYL KF E+YP +R AI P +Y
Sbjct: 302 RWYLQKF-ENYPASRHAIFPHVY 320

[Close]

BLAST of MC05g1521 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-09
Identity = 33/83 (39.76%), Postives = 43/83 (51.81%), Query Frame = 0


Query: 181 YKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWS-----WAGLGFFLYTCANLVPRARANH 240
           Y +P G WF +VS P++L E+V + G  +         W   GF     ANL   A   H
Sbjct: 265 YIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF---VAANLTYAAGETH 324

Query: 241 TWYLNKFGEDYPTTRKAIIPFLY 259
            WYL KF E+YP  R AI P++Y
Sbjct: 325 RWYLRKF-ENYPANRHAIFPYVY 343

[Close]

BLAST of MC05g1521 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-09
Identity = 33/83 (39.76%), Postives = 43/83 (51.81%), Query Frame = 0


Query: 181 YKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWS-----WAGLGFFLYTCANLVPRARANH 240
           Y +P G WF +VS P++L E+V + G  +         W   GF     ANL   A   H
Sbjct: 264 YIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF---VAANLTYAAGETH 323

Query: 241 TWYLNKFGEDYPTTRKAIIPFLY 259
            WYL KF E+YP  R AI P++Y
Sbjct: 324 RWYLRKF-ENYPANRHAIFPYVY 342

The following BLAST results are available for this feature:

BLAST of MC05g1521 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q38944	4.7e-103	66.28	Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2	[more]
Q2QDF6	1.4e-102	67.44	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
Q5K2N1	8.0e-87	56.59	Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1	[more]
Q28891	2.9e-36	38.36	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
Q68FF9	4.9e-36	40.43	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]

BLAST of MC05g1521 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145671.1	1.70e-196	100.00	steroid 5-alpha-reductase DET2 [Momordica charantia]	[more]
XP_038905208.1	2.92e-166	85.33	LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida]	[more]
KAA0044147.1	4.14e-166	84.17	steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-...	[more]
XP_008442471.1	9.32e-166	83.52	PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo]	[more]
XP_004137737.1	2.19e-164	83.52	steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protei...	[more]

BLAST of MC05g1521 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CX04	8.23e-197	100.00	Steroid 5-alpha-reductase DET2 OS=Momordica charantia OX=3673 GN=LOC111015065 PE...	[more]
A0A5D3DNY2	2.01e-166	84.17	Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3B6E3	4.51e-166	83.52	Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1	[more]
A0A0A0LAB3	1.06e-164	83.52	Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 S...	[more]
A0A6J1J7F2	3.18e-163	84.56	Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 ...	[more]

BLAST of MC05g1521 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38050.1	3.3e-104	66.28	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	6.0e-13	27.89	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	6.6e-12	43.37	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	1.1e-09	39.76	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.2	1.1e-09	39.76	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016636	3-oxo-5-alpha-steroid 4-dehydrogenase	PIRSF	PIRSF015596	5_alpha-SR2	coord: 1..258 e-value: 1.9E-88 score: 294.0
None	No IPR available	GENE3D	1.20.120.1630		coord: 80..257 e-value: 2.7E-12 score: 48.8
None	No IPR available	PANTHER	PTHR10556:SF53	STEROID 5-ALPHA-REDUCTASE DET2	coord: 5..258
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 111..258 e-value: 2.8E-50 score: 170.2
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 153..232 score: 27.570135
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 5..258

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g1521.1	MC05g1521.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0008202	steroid metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0047751	cholestenone 5-alpha-reductase activity
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors