MC05g1339 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g1339
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionpurine-uracil permease NCS1
LocationMC05: 17640416 .. 17642080 (+)
RNA-Seq ExpressionMC05g1339
SyntenyMC05g1339
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGACTCAGTCCATTAACCTCCGTCTCCACCACCACATTCTCCACCTCTCCCCTTCGATCTCAGCCCACCGGCACCGCTCCGTCGTCTCTCAGGTACCTTTCTCTCCGATTAGCCCCAAATTGCTTCTTAAACGACGCTCTAATTTCACCCGCATGGCCTCCGCCGCCGGCCGACCGACCAACGACCCGCATTTCCGCGATGCCGACTCTGCTCCGACGCTCGGCAGCGAAGATCTCCGGCCTACGACGCCGGAGCAGAGAACGCTCGCCTGGCCGGAGATGGCCACGCTTTGGGTGGGGCTGATCGTCGGCGTCCCGGCTTATTACCTGGCCGGAAGCTTGGTCGACCTCGGAATGGCGTGGTGGCAGGGCATCGCCACCGTCGTCGCCGCCAACGCCGTCACCGTAGCGGCGCTGGTTCTCACCGGCCATCCGGGGACCAAATACGGCATATCGTTCCCGATTCTTGCCCGATCGTCGTTCGGAATCCACGGCGCCCACGTCCCCACGCTTCTTCGAGCCCTAATCGGCTGCGGCTGGTACGGAATCGAAACATGGATCGCAGGCGAGGCGATTTTCATTCTATTACCGGAAAGTATCAAGGAATCTTCCTTCTCCCATGTTCTTCCATGGCTGGGAACTTCCCCTCTCGCGTTTTCTTGCTTCTTGGCGTTCTGGGTCGTTCAATTGGCCAGCGTTTGGAAAGGAATGGACGGAATTCGAAAACTGGAAACGTATTCTGCCCCAATTCTTGTTTTCCTCACGTTTGGGCTCTTGATTTGGTCTTATGTTAGGGCCGGTGGATTAGGGCATATGCTGTCTTTAACCTCGAAATTGTCTGCGTCGGAATTTTGGGCTTTGTTTTTCCCCTCTCTTACTGCTAACATAAGCTTTTGGGCGACTCTGGCTTTGAATATCCCCGATTTCACTCGCTACGCTAGAACCCAAAAGGACCAAATCCTCGGCCAATTAGGGCTTCCGATTTTCATGGGGGCGTTCACTTTCGTCGGCGTCGCCGTCACCTCCTCCACCGGGGTCATTTTCGGCCGGGTGATTTCCAACCCGATTGACCTTCTCGGCCACATCGGCGGATTCACCACCAAGGTTTTGGCGATTTTTGGGATCATCCTGGCCACCGTGACCACCAACGTCGCCGCCAATGTGGTGGCGCCGGCGAACGCGCTGGTGAATCTCAGCCCTTCTGTGTTCAGTTTCACGAGAGGCGCCGTGCTCACCGCCGTGGTCGGCATCGTCTTCCAGCCGGGGAGGCTTCTGAGCTCCAGCGAGAGCTTCGTTTACACTTGGCTTGTTGGGTACTCGGCGCTTTTGGGTCCGATCGGCGGGATAATGCTGACGGATTACTATCTGATCCGCCAGAGAGAATTGAGCGTCGAAGACTTGTATTCGTCGAGTCCTTCGGGGGCTTATTACTACTCCGGCGGGTTCAATTTGGCGGCGATGGCGGCGCTGGTCGTCGGAGTGTTGCCGGTGATTCCTGGGTTTTTGGAGAAGGTGGAGATCGTGTCTAGGGTTCCGAAAGTATTCGGAATTATTTACGGAAACGCCTGGTTTATAAGCTCCTTTTTGGCTGGTTTTTGCTATTGGGGTCTCTCTGTTCTGTTGAAACGGCAAAAAGTTCCTCGCCCTTTGGACCCACTTCTT

mRNA sequence

ATGGTGACTCAGTCCATTAACCTCCGTCTCCACCACCACATTCTCCACCTCTCCCCTTCGATCTCAGCCCACCGGCACCGCTCCGTCGTCTCTCAGGTACCTTTCTCTCCGATTAGCCCCAAATTGCTTCTTAAACGACGCTCTAATTTCACCCGCATGGCCTCCGCCGCCGGCCGACCGACCAACGACCCGCATTTCCGCGATGCCGACTCTGCTCCGACGCTCGGCAGCGAAGATCTCCGGCCTACGACGCCGGAGCAGAGAACGCTCGCCTGGCCGGAGATGGCCACGCTTTGGGTGGGGCTGATCGTCGGCGTCCCGGCTTATTACCTGGCCGGAAGCTTGGTCGACCTCGGAATGGCGTGGTGGCAGGGCATCGCCACCGTCGTCGCCGCCAACGCCGTCACCGTAGCGGCGCTGGTTCTCACCGGCCATCCGGGGACCAAATACGGCATATCGTTCCCGATTCTTGCCCGATCGTCGTTCGGAATCCACGGCGCCCACGTCCCCACGCTTCTTCGAGCCCTAATCGGCTGCGGCTGGTACGGAATCGAAACATGGATCGCAGGCGAGGCGATTTTCATTCTATTACCGGAAAGTATCAAGGAATCTTCCTTCTCCCATGTTCTTCCATGGCTGGGAACTTCCCCTCTCGCGTTTTCTTGCTTCTTGGCGTTCTGGGTCGTTCAATTGGCCAGCGTTTGGAAAGGAATGGACGGAATTCGAAAACTGGAAACGTATTCTGCCCCAATTCTTGTTTTCCTCACGTTTGGGCTCTTGATTTGGTCTTATGTTAGGGCCGGTGGATTAGGGCATATGCTGTCTTTAACCTCGAAATTGTCTGCGTCGGAATTTTGGGCTTTGTTTTTCCCCTCTCTTACTGCTAACATAAGCTTTTGGGCGACTCTGGCTTTGAATATCCCCGATTTCACTCGCTACGCTAGAACCCAAAAGGACCAAATCCTCGGCCAATTAGGGCTTCCGATTTTCATGGGGGCGTTCACTTTCGTCGGCGTCGCCGTCACCTCCTCCACCGGGGTCATTTTCGGCCGGGTGATTTCCAACCCGATTGACCTTCTCGGCCACATCGGCGGATTCACCACCAAGGTTTTGGCGATTTTTGGGATCATCCTGGCCACCGTGACCACCAACGTCGCCGCCAATGTGGTGGCGCCGGCGAACGCGCTGGTGAATCTCAGCCCTTCTGTGTTCAGTTTCACGAGAGGCGCCGTGCTCACCGCCGTGGTCGGCATCGTCTTCCAGCCGGGGAGGCTTCTGAGCTCCAGCGAGAGCTTCGTTTACACTTGGCTTGTTGGGTACTCGGCGCTTTTGGGTCCGATCGGCGGGATAATGCTGACGGATTACTATCTGATCCGCCAGAGAGAATTGAGCGTCGAAGACTTGTATTCGTCGAGTCCTTCGGGGGCTTATTACTACTCCGGCGGGTTCAATTTGGCGGCGATGGCGGCGCTGGTCGTCGGAGTGTTGCCGGTGATTCCTGGGTTTTTGGAGAAGGTGGAGATCGTGTCTAGGGTTCCGAAAGTATTCGGAATTATTTACGGAAACGCCTGGTTTATAAGCTCCTTTTTGGCTGGTTTTTGCTATTGGGGTCTCTCTGTTCTGTTGAAACGGCAAAAAGTTCCTCGCCCTTTGGACCCACTTCTT

Coding sequence (CDS)

ATGGTGACTCAGTCCATTAACCTCCGTCTCCACCACCACATTCTCCACCTCTCCCCTTCGATCTCAGCCCACCGGCACCGCTCCGTCGTCTCTCAGGTACCTTTCTCTCCGATTAGCCCCAAATTGCTTCTTAAACGACGCTCTAATTTCACCCGCATGGCCTCCGCCGCCGGCCGACCGACCAACGACCCGCATTTCCGCGATGCCGACTCTGCTCCGACGCTCGGCAGCGAAGATCTCCGGCCTACGACGCCGGAGCAGAGAACGCTCGCCTGGCCGGAGATGGCCACGCTTTGGGTGGGGCTGATCGTCGGCGTCCCGGCTTATTACCTGGCCGGAAGCTTGGTCGACCTCGGAATGGCGTGGTGGCAGGGCATCGCCACCGTCGTCGCCGCCAACGCCGTCACCGTAGCGGCGCTGGTTCTCACCGGCCATCCGGGGACCAAATACGGCATATCGTTCCCGATTCTTGCCCGATCGTCGTTCGGAATCCACGGCGCCCACGTCCCCACGCTTCTTCGAGCCCTAATCGGCTGCGGCTGGTACGGAATCGAAACATGGATCGCAGGCGAGGCGATTTTCATTCTATTACCGGAAAGTATCAAGGAATCTTCCTTCTCCCATGTTCTTCCATGGCTGGGAACTTCCCCTCTCGCGTTTTCTTGCTTCTTGGCGTTCTGGGTCGTTCAATTGGCCAGCGTTTGGAAAGGAATGGACGGAATTCGAAAACTGGAAACGTATTCTGCCCCAATTCTTGTTTTCCTCACGTTTGGGCTCTTGATTTGGTCTTATGTTAGGGCCGGTGGATTAGGGCATATGCTGTCTTTAACCTCGAAATTGTCTGCGTCGGAATTTTGGGCTTTGTTTTTCCCCTCTCTTACTGCTAACATAAGCTTTTGGGCGACTCTGGCTTTGAATATCCCCGATTTCACTCGCTACGCTAGAACCCAAAAGGACCAAATCCTCGGCCAATTAGGGCTTCCGATTTTCATGGGGGCGTTCACTTTCGTCGGCGTCGCCGTCACCTCCTCCACCGGGGTCATTTTCGGCCGGGTGATTTCCAACCCGATTGACCTTCTCGGCCACATCGGCGGATTCACCACCAAGGTTTTGGCGATTTTTGGGATCATCCTGGCCACCGTGACCACCAACGTCGCCGCCAATGTGGTGGCGCCGGCGAACGCGCTGGTGAATCTCAGCCCTTCTGTGTTCAGTTTCACGAGAGGCGCCGTGCTCACCGCCGTGGTCGGCATCGTCTTCCAGCCGGGGAGGCTTCTGAGCTCCAGCGAGAGCTTCGTTTACACTTGGCTTGTTGGGTACTCGGCGCTTTTGGGTCCGATCGGCGGGATAATGCTGACGGATTACTATCTGATCCGCCAGAGAGAATTGAGCGTCGAAGACTTGTATTCGTCGAGTCCTTCGGGGGCTTATTACTACTCCGGCGGGTTCAATTTGGCGGCGATGGCGGCGCTGGTCGTCGGAGTGTTGCCGGTGATTCCTGGGTTTTTGGAGAAGGTGGAGATCGTGTCTAGGGTTCCGAAAGTATTCGGAATTATTTACGGAAACGCCTGGTTTATAAGCTCCTTTTTGGCTGGTTTTTGCTATTGGGGTCTCTCTGTTCTGTTGAAACGGCAAAAAGTTCCTCGCCCTTTGGACCCACTTCTT

Protein sequence

MVTQSINLRLHHHILHLSPSISAHRHRSVVSQVPFSPISPKLLLKRRSNFTRMASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQPGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCYWGLSVLLKRQKVPRPLDPLL
Homology
BLAST of MC05g1339 vs. ExPASy Swiss-Prot
Match: Q9LZD0 (Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 3.2e-202
Identity = 346/515 (67.18%), Postives = 421/515 (81.75%), Query Frame = 0

Query: 35  FSPISP--KLLLKRRSNF-TRMASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLA 94
           FSPI P   L+L R      R+++  G   ND  + ++   P+L ++DL+PTTP QRT +
Sbjct: 74  FSPILPGDNLVLSRSGVIRPRLSAMTGSEINDHGYDESQFDPSLTNDDLKPTTPSQRTFS 133

Query: 95  WPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYG 154
           W +M++LW+GL+VGVP YYLAGSLVDLGMAWWQGIATVV AN + +  LVLT  PGT YG
Sbjct: 134 WLDMSSLWIGLVVGVPTYYLAGSLVDLGMAWWQGIATVVTANLILLVPLVLTAQPGTLYG 193

Query: 155 ISFPILARSSFGIHGAHVPTLLRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLP 214
           ISFP+LARSSFGI GAH+PTLLRAL+GCGWYGIETWI GEAIF+LLP  IK+S+ SH LP
Sbjct: 194 ISFPVLARSSFGIRGAHIPTLLRALVGCGWYGIETWIGGEAIFLLLPGHIKKSALSHTLP 253

Query: 215 WLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLG 274
           WLGTSPL FSCF+ FW+ QL  VW+GMDGIRKLE YSAPIL+ LT  LL WSY++AGG G
Sbjct: 254 WLGTSPLEFSCFIVFWLAQLCIVWRGMDGIRKLEKYSAPILISLTSCLLAWSYLKAGGFG 313

Query: 275 HMLSLTSKLSASEFWALFFPSLTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFM 334
           HMLSL+SKL++++FW LFFPSLTANISFWATLALNIPDF+R+A++Q DQI+GQ+GLP+FM
Sbjct: 314 HMLSLSSKLTSAQFWTLFFPSLTANISFWATLALNIPDFSRFAKSQTDQIIGQVGLPVFM 373

Query: 335 GAFTFVGVAVTSSTGVIFGRVISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVA 394
           G FTFVGVAVTSST +IFGRVISNPI+LLG IGG  T +LAI GI LAT+TTN+AANVVA
Sbjct: 374 GLFTFVGVAVTSSTSIIFGRVISNPIELLGQIGGLATTLLAIVGISLATLTTNIAANVVA 433

Query: 395 PANALVNLSPSVFSFTRGAVLTAVVGIVFQPGRLLSSSESFVYTWLVGYSALLGPIGGIM 454
           PANALVNL+P  F+F RGA LTAV+GIVFQP RLL SSESFVYTWL+GYSALLGPIGGI+
Sbjct: 434 PANALVNLNPKFFTFGRGAFLTAVLGIVFQPWRLLKSSESFVYTWLIGYSALLGPIGGII 493

Query: 455 LTDYYLIRQRELSVEDLYSSSPSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSR 514
           L DYYLI++ +L++ DLYS SPSG YY+S G+N+AA+ ALV G++PV+PGFL K+  +S+
Sbjct: 494 LVDYYLIKKMKLNIGDLYSLSPSGEYYFSKGYNVAAVVALVAGIIPVVPGFLHKISALSK 553

Query: 515 VPKVFGIIYGNAWFISSFLAGFCYWGLSVLLKRQK 547
           +   F ++Y NA F S  +AGF YW +   L R++
Sbjct: 554 ISNGFVVVYDNALFFSFIIAGFVYWIIMSRLGRKQ 588

BLAST of MC05g1339 vs. ExPASy Swiss-Prot
Match: P94575 (Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.7e-78
Identity = 179/482 (37.14%), Postives = 267/482 (55.39%), Query Frame = 0

Query: 75  LGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANA 134
           L +EDL P   E+RT      A++W+G I  +P Y   G L+ +G++ WQ +A ++ A+ 
Sbjct: 13  LSNEDLVPLGQEKRTWKAMNFASIWMGCIHNIPTYATVGGLIAIGLSPWQVLAIIITASL 72

Query: 135 VTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGWYGIETWIAGEAIF 194
           +   AL L GH GTKYG+ FP++ R+S+GI+GA++P LLRA     W GI+T+    A+ 
Sbjct: 73  ILFGALALNGHAGTKYGLPFPVIIRASYGIYGANIPALLRAFTAIMWLGIQTFAGSTALN 132

Query: 195 ILLPESIKE-SSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPILV 254
           ILL                LG        F+ FW + L  +  GM+ I++ E ++ P LV
Sbjct: 133 ILLLNMWPGWGEIGGEWNILGIHLSGLLSFVFFWAIHLLVLHHGMESIKRFEVWAGP-LV 192

Query: 255 FLTFGLLIWSYVR-AGGLGHMLSLTSKLS--ASEFWALFFPSLTANISFWATLALNIPDF 314
           +L FG ++W  V  AGGLG + S   K    +  FW  F   +T  I  WATL LNIPDF
Sbjct: 193 YLVFGGMVWWAVDIAGGLGPIYSQPGKFHTFSETFWP-FAAGVTGIIGIWATLILNIPDF 252

Query: 315 TRYARTQKDQILGQL-GLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLLGHIGGFTTK 374
           TR+A TQK+QI GQ  GLP     F F  + VTS + V FG  I + +D+L         
Sbjct: 253 TRFAETQKEQIKGQFYGLPGTFALFAFASITVTSGSQVAFGEPIWDVVDILARFDNPYVI 312

Query: 375 VLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQPGRLLSSS 434
           VL++  + +AT++ NVAAN+V+PA  + N  P   +F RG+ +TA++ +   P +L+ S+
Sbjct: 313 VLSVITLCIATISVNVAANIVSPAYDIANALPKYINFKRGSFITALLALFTVPWKLMESA 372

Query: 435 ESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSGAYYYSGGFNLAAMA 494
            S VY +L     +LGP+ G+M+ DY++IR+RELSV+DLYS   +G Y Y  G+N  A A
Sbjct: 373 TS-VYAFLGLIGGMLGPVAGVMMADYFIIRKRELSVDDLYSE--TGRYVYWKGYNYRAFA 432

Query: 495 ALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCYWGLSVLLKRQKVPR 552
           A ++G L         + ++     V   +Y  +WF+   ++   Y    ++L R   P 
Sbjct: 433 ATMLGAL---------ISLIGMYVPVLKSLYDISWFVGVLISFLFY----IVLMRVHPPA 476

BLAST of MC05g1339 vs. ExPASy Swiss-Prot
Match: O14035 (Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC29B12.14c PE=3 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 1.2e-58
Identity = 154/452 (34.07%), Postives = 234/452 (51.77%), Query Frame = 0

Query: 75  LGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANA 134
           L +EDL P    QR          W+   V V  + +AG+ V+ G++WW+   TV     
Sbjct: 48  LTNEDLYPVPKSQRVWGPWNYVAFWLADSVNVNTWMIAGTAVESGLSWWEAWITVWVGYT 107

Query: 135 VTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGWYGIETWIAGEAIF 194
           +    L + G  G  Y ISFP+L+RSSFGI G+  P L RA++ C WYG++ WI GE + 
Sbjct: 108 IAAFILTIAGRAGAVYHISFPVLSRSSFGIWGSLWPILNRAVMACVWYGVQAWIGGECVT 167

Query: 195 ILLPESIKESSFSHV---LPWLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPI 254
           +++  SI   SFSH+   +   GT    +  F  FW++   ++W  +  IR L T  + +
Sbjct: 168 LMI-RSI-WPSFSHIPNTMAKSGTETYQWVGFFIFWLISNVAIWFPVYQIRHLFTAKSFL 227

Query: 255 LVFLTFGLLIWSYVRAGGLGHMLSLTSKLSA-SEFWALFFPSLTANISFWATLALNIPDF 314
                   LIW+ V+A G G  +   ++LS  +  WA+    + + +  +ATL +N PDF
Sbjct: 228 APPAAIAFLIWALVKAHGAGDAIHAKTQLSTWNHGWAV-TAGIISCLDNFATLIVNNPDF 287

Query: 315 TRYARTQKDQILGQL-GLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLL-------GH 374
           TR+A T    I  QL  +P+  G  T +GV V S++  I+G  I NP+DLL        H
Sbjct: 288 TRFATTPNAPIFPQLITIPMGFGITTLIGVLVGSASKSIYGENIWNPLDLLKSFLDHSNH 347

Query: 375 IGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQP 434
            G          G+ LA +  N+AAN V+  N    L P   +  RG  + +++GI   P
Sbjct: 348 HGVRAGVFFISTGLCLAQLGVNIAANTVSAGNDTSALCPMFINIRRGGYIASIIGICMCP 407

Query: 435 GRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSGAYYYSGG 494
             LLSSS SF  + L  Y+  L    GI++ DY++IR+  L V+ LY+ +P+  Y+++ G
Sbjct: 408 WNLLSSSNSFANS-LSAYAVFLSSFAGILIADYFVIRKGYLKVDALYTINPNEPYWFTYG 467

Query: 495 FNLAAMAALVVGVLPVIPGFLEKVEIVSRVPK 515
            NL A A+ + G+L  + G    V    +VPK
Sbjct: 468 INLRAFASYICGLLINVVGLAGAVG--DKVPK 493

BLAST of MC05g1339 vs. ExPASy Swiss-Prot
Match: Q10279 (Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fur4 PE=1 SV=2)

HSP 1 Score: 224.2 bits (570), Expect = 3.7e-57
Identity = 148/468 (31.62%), Postives = 238/468 (50.85%), Query Frame = 0

Query: 75  LGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANA 134
           L ++DL P  P +R        + W+   V +  + ++ + ++LG+ WW+    V     
Sbjct: 41  LTNKDLYPVPPSKRLWGPWNFISFWLADAVNINTWMISATAIELGLNWWEAWICVWVGYL 100

Query: 135 VTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGWYGIETWIAGEAIF 194
           +    +  TG PG  Y ISFP+L+RSSFG  G+  P L R+++ C WYG++ WI GE + 
Sbjct: 101 ICGILVATTGRPGAVYHISFPVLSRSSFGTWGSLWPILNRSVLACVWYGVQAWIGGECV- 160

Query: 195 ILLPESIKESSFSHV---LPWLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPI 254
           +L+  SI   SFSH+   +   GT    +  F  FW++   ++W  +  IR L T+ A +
Sbjct: 161 VLMIRSI-WPSFSHIPNTMAKSGTETYQWVGFFIFWLLSNIAIWFPVHQIRHLFTFKAIV 220

Query: 255 LVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTANISFWATLALNIPDFT 314
                   LIW+ V+A G G ++   +KL   E   +    +   I  +ATL +N PDF 
Sbjct: 221 APPAAIAFLIWALVKAHGAGPVIHEPTKLGQYEHAWVVINGIVTCIDGFATLIVNNPDFA 280

Query: 315 RYARTQKDQILGQ-LGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLL------GHIG 374
           R+A T       Q + +P+  G  + +GV V+S++  I+G  + +P  LL       +  
Sbjct: 281 RFATTPGAVHWPQIITVPLAFGVTSLIGVLVSSASKAIYGTTLWDPTQLLASFLDHSNAH 340

Query: 375 GFTTKVLAI-FGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQPG 434
           G    V  I FG+ +A +  N+AAN V+  N L  L P+V +  RG  + +++ +   P 
Sbjct: 341 GVRAGVFFIAFGLCIAQLGVNIAANSVSAGNDLSALLPTVINVRRGGYIASIIALCMCPW 400

Query: 435 RLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSGAYYYSGGF 494
            LLSS+ +F  T+L  YS  L    G+++ DYY +R+  + V  LYSSS S  YY+  G 
Sbjct: 401 NLLSSNNNFT-TYLSSYSVFLSSFAGVIIADYYFVRKGLIRVAPLYSSSSSSPYYFWKGI 460

Query: 495 NLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLA 532
           N  A A+ + G+L  I G         +VPKV   ++   +F+   +A
Sbjct: 461 NFRAFASYICGMLINIVGMAGSTG--QKVPKVANTMFNLNYFLGITVA 503

BLAST of MC05g1339 vs. ExPASy Swiss-Prot
Match: P75712 (Putative allantoin permease OS=Escherichia coli (strain K12) OX=83333 GN=ybbW PE=1 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 4.3e-45
Identity = 122/449 (27.17%), Postives = 229/449 (51.00%), Query Frame = 0

Query: 77  SEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVT 136
           SEDL P T  QRT       TLW+G +  VP Y + G    LG++ +  +  ++ +    
Sbjct: 14  SEDLLPKTQSQRTWKTFNYFTLWMGSVHNVPNYVMVGGFFILGLSTFSIMLAIILSAFFI 73

Query: 137 VAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLR-ALIGCGWYGIETWIAGEAIFI 196
            A +VL G  G+KYG+ F ++ R+S+G+ GA  P LLR  +    W+G++ +    A  I
Sbjct: 74  AAVMVLNGAAGSKYGVPFAMILRASYGVRGALFPGLLRGGIAAIMWFGLQCYAGSLACLI 133

Query: 197 LLPESIKE-SSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPILVF 256
           L+ +      +       LG S      FL FW+V +   + G   + K      P +  
Sbjct: 134 LIGKIWPGFLTLGGDFTLLGLSLPGLITFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYI 193

Query: 257 LTFGLLIWSYVRAGGLGHMLS-LTSKLSASEFWA-LFFPSLTANISFWATLALNIPDFTR 316
           +  G+ IW+ +   G+G +   + S +  +E    LF   + A ++ WA  A++  DFT+
Sbjct: 194 VFGGMAIWA-ISLVGIGPIFDYIPSGIQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQ 253

Query: 317 YARTQKDQILGQ-LGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLLGHIGGFTTKVL 376
            A + ++Q LGQ LGL +    F   GV + +   + +G    N +D++           
Sbjct: 254 NAHSFREQALGQTLGLVVAYILFAVAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFF 313

Query: 377 AIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQPGRLLSSSES 436
           A+  I++ T++TN   N++     +  ++P+  ++  G ++ +++ ++  P +L+ + +S
Sbjct: 314 AVLVILMTTISTNATGNIIPAGYQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDS 373

Query: 437 FVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSGAYYYSGGFNLAAMAAL 496
            +Y +L     +LGP+ G+M+  Y+++ + ++++++LY ++P    YY  GFNL A +  
Sbjct: 374 -IYLFLDIIGGMLGPVIGVMMAHYFVVMRGQINLDELY-TAPGDYKYYDNGFNLTAFSVT 433

Query: 497 VVGVLPVIPG-FLEKVEIVSRVPKVFGII 520
           +V V+  + G F+  +E +SRV    G+I
Sbjct: 434 LVAVILSLGGKFIHFMEPLSRVSWFVGVI 459

BLAST of MC05g1339 vs. NCBI nr
Match: XP_038906223.1 (purine-uracil permease NCS1 [Benincasa hispida])

HSP 1 Score: 929 bits (2401), Expect = 0.0
Identity = 472/564 (83.69%), Postives = 513/564 (90.96%), Query Frame = 0

Query: 1   MVTQSINLRLHHHILHLSPSISA----HRHRSVVSQVP--FSPISPKLLLKRRSN--FTR 60
           MVTQ INL LHHHI HL PS+SA    HRH   VSQ P  FSP++PKLLLKRRSN  FT 
Sbjct: 1   MVTQCINLHLHHHIFHLPPSLSALRHRHRHHPTVSQSPLLFSPVTPKLLLKRRSNLKFTP 60

Query: 61  -MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYL 120
            MASA   P+ D    D+      G EDLRPTTPEQRTL+WPEMA LWVGLIVGVPAYYL
Sbjct: 61  PMASAVDHPSEDTQNEDS------GREDLRPTTPEQRTLSWPEMALLWVGLIVGVPAYYL 120

Query: 121 AGSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPT 180
           AGSLVDLGM+WWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+ ARSSFGIHGAH+PT
Sbjct: 121 AGSLVDLGMSWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPT 180

Query: 181 LLRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQL 240
           LLRALIGCGWYGIETWIAGEAIF+LLPESIK+SSFS VLPWLGTSPLAF CFLAFW+VQL
Sbjct: 181 LLRALIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFICFLAFWIVQL 240

Query: 241 ASVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFP 300
           ASVWKGMDGIRKLE +SAPILVFLT  LLIWS VRAGGLGHMLSLTSKLS+SEFWALFFP
Sbjct: 241 ASVWKGMDGIRKLEIFSAPILVFLTVCLLIWSCVRAGGLGHMLSLTSKLSSSEFWALFFP 300

Query: 301 SLTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGR 360
           SLTANISFWATLALNIPDFTRYA++QKDQI+GQLGLPIFMGAFTFVGVAVTSSTG+IFG 
Sbjct: 301 SLTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGLIFGH 360

Query: 361 VISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAV 420
           +ISNPI+LLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNL+PS F+FTRGA+
Sbjct: 361 IISNPIELLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGAL 420

Query: 421 LTAVVGIVFQPGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSS 480
           LTAV+GI+FQP RLLSSSESFVYTWLVGYSALLGPIGG+M+TDYYLIR+RELSVEDLYS+
Sbjct: 421 LTAVIGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYST 480

Query: 481 SPSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLA 540
           SP+GAYYY+GGFNLAA+AALVVGVLPVIPGFLEKV+IVS+VPKVF +IYGNAWFIS+FLA
Sbjct: 481 SPTGAYYYTGGFNLAAVAALVVGVLPVIPGFLEKVKIVSKVPKVFSVIYGNAWFISTFLA 540

Query: 541 GFCYWGLSVLLKRQKVPRPLDPLL 555
           GFCYWGLS+LLKR+K+ R   PLL
Sbjct: 541 GFCYWGLSILLKRRKLSRRFGPLL 558

BLAST of MC05g1339 vs. NCBI nr
Match: XP_008442760.1 (PREDICTED: purine-uracil permease NCS1 [Cucumis melo] >KAA0043951.1 purine-uracil permease NCS1 [Cucumis melo var. makuwa] >TYK25190.1 purine-uracil permease NCS1 [Cucumis melo var. makuwa])

HSP 1 Score: 910 bits (2352), Expect = 0.0
Identity = 464/560 (82.86%), Postives = 501/560 (89.46%), Query Frame = 0

Query: 1   MVTQSINLRLHHHILHLSPSISAHRHRSVVSQVP--FSPISPKLLLKRRSN--FTR-MAS 60
           MVT  INL LH HI HL PSIS  R+R  VS  P  FSPI+PKLLLKRRSN  FT  MAS
Sbjct: 1   MVTHCINLHLHRHIFHLPPSISTLRYRPTVSHSPLLFSPITPKLLLKRRSNLRFTSPMAS 60

Query: 61  AAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSL 120
           A   P+ D    D+      G +DLRPTT EQRTL+WPEMA LW+GLIVGVP+YYLAGSL
Sbjct: 61  ADANPSEDTQNDDS------GKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSL 120

Query: 121 VDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRA 180
           VDLGM+WWQGIATVV ANAVT+ AL+L GHPGTKYGISFP+ ARSSFGIHGAH+PTLLRA
Sbjct: 121 VDLGMSWWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRA 180

Query: 181 LIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVW 240
           LIGCGWYGIETWIAGEAIF+LLPESIK+SSFS +LPWLGTSPLAF+CFLAFWVVQLASVW
Sbjct: 181 LIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLASVW 240

Query: 241 KGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTA 300
           KGMDGIRKLET+SAPILVFLT  LLIWS V+AGG GHMLSLTSKLS+SEFWALFFPSLTA
Sbjct: 241 KGMDGIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPSLTA 300

Query: 301 NISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISN 360
           NISFWATLALNIPDFTRYA++QKDQI+GQLGLPIFMGAFTFVGVAVTSSTGVIFG +ISN
Sbjct: 301 NISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISN 360

Query: 361 PIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAV 420
           PIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+LTAV
Sbjct: 361 PIDLLGHIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAV 420

Query: 421 VGIVFQPGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSG 480
           +GI+FQP RLLSSSESFVYTWLVGYSALLGPIGG+M+TDYYLIR +ELSVEDLYS+SP G
Sbjct: 421 IGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTSPRG 480

Query: 481 AYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCY 540
           AYYYSGGFNL A+ ALVVGVLPVIPGFLEKVEIVS+VPKVF +IYG+AWFISSFLAGFCY
Sbjct: 481 AYYYSGGFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAGFCY 540

Query: 541 WGLSVLLKRQKVPRPLDPLL 555
           WGLSVLLKR+K      PLL
Sbjct: 541 WGLSVLLKRRKASLRFGPLL 554

BLAST of MC05g1339 vs. NCBI nr
Match: XP_023539092.1 (purine-uracil permease NCS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 456/552 (82.61%), Postives = 497/552 (90.04%), Query Frame = 0

Query: 6   INLRLHHHILHLSPSISAHRHRSVVSQVPFSPISPKLLLKRR----SNFTRMASAAGRPT 65
           ++L LHHH   L P++S  R    VS + FSPI+PKLLL+ R    +NFT MASAA  PT
Sbjct: 18  VSLHLHHHNFRLPPALSTVRRHPTVSLLHFSPITPKLLLRHRFKSNTNFTAMASAADNPT 77

Query: 66  NDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMA 125
           ++ H  ++      GS+DLRPTTPEQRTLAWPEMA LW+GL+VGVPAYYLAGSLVDLGMA
Sbjct: 78  DNTHVENS------GSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMA 137

Query: 126 WWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGW 185
           WWQGIATVVAANAVT+ AL+LTGHPGTKYGISFPI ARSSFGIHGAH+PTLLRALIGCGW
Sbjct: 138 WWQGIATVVAANAVTLIALILTGHPGTKYGISFPIFARSSFGIHGAHIPTLLRALIGCGW 197

Query: 186 YGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDGI 245
           YGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLASVWKGMDGI
Sbjct: 198 YGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 257

Query: 246 RKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTANISFWA 305
           RKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPSLTANISFWA
Sbjct: 258 RKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWA 317

Query: 306 TLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLLG 365
           TLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +ISNPIDLLG
Sbjct: 318 TLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLG 377

Query: 366 HIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQ 425
           HIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+LTAV+GIVFQ
Sbjct: 378 HIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQ 437

Query: 426 PGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSGAYYYSG 485
           P RLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY IR+REL VEDLYS SP+GAYYYSG
Sbjct: 438 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSLSPTGAYYYSG 497

Query: 486 GFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCYWGLSVL 545
           GFNL A+ ALVVGVLPVIPGFLEKV+IVS+VP+VF +IYGNAWFIS+F+AGFCYWGLSVL
Sbjct: 498 GFNLTAVTALVVGVLPVIPGFLEKVKIVSKVPQVFTVIYGNAWFISTFIAGFCYWGLSVL 557

Query: 546 LKRQKVPRPLDP 553
           LKR+KV   L P
Sbjct: 558 LKRRKVSSCLGP 563

BLAST of MC05g1339 vs. NCBI nr
Match: XP_023005190.1 (purine-uracil permease NCS1-like [Cucurbita maxima])

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 454/552 (82.25%), Postives = 494/552 (89.49%), Query Frame = 0

Query: 6   INLRLHHHILHLSPSISAHRHRSVVSQVPFSPISPKLLLKRR----SNFTRMASAAGRPT 65
           +NL LH H     P ++  R    VS + FSPI+PKLLL+ R    +NF  MASAA  PT
Sbjct: 18  VNLHLHRHNFRFPPCLTTVRRHPTVSLLRFSPITPKLLLRHRFKSNTNFIPMASAANNPT 77

Query: 66  NDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMA 125
           ++ H  +       GS+DLRPTTPEQRTLAWPEMA LW+GL+VGVPAYYLAGSLVDLGMA
Sbjct: 78  DNTHVENP------GSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMA 137

Query: 126 WWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGW 185
           WWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+ ARSSFGIHGAH+PTLLRALIGCGW
Sbjct: 138 WWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 197

Query: 186 YGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDGI 245
           YGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLASVWKGMDGI
Sbjct: 198 YGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 257

Query: 246 RKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTANISFWA 305
           RKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPSLTANISFWA
Sbjct: 258 RKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWA 317

Query: 306 TLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLLG 365
           TLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +ISNPIDLLG
Sbjct: 318 TLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLG 377

Query: 366 HIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQ 425
           HIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+LTAV+GIVFQ
Sbjct: 378 HIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQ 437

Query: 426 PGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSGAYYYSG 485
           P RLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY +R+REL VEDLYSSSP+GAYYYSG
Sbjct: 438 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFLRKRELIVEDLYSSSPTGAYYYSG 497

Query: 486 GFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCYWGLSVL 545
           GFNL A+AALVVGVLPVIPGFLEKV+IVS+VP+VF +IYGNAWFISSF+AGFCYWGLSVL
Sbjct: 498 GFNLTAVAALVVGVLPVIPGFLEKVKIVSKVPEVFTVIYGNAWFISSFIAGFCYWGLSVL 557

Query: 546 LKRQKVPRPLDP 553
           LKR+KV   L P
Sbjct: 558 LKRRKVSSCLGP 563

BLAST of MC05g1339 vs. NCBI nr
Match: KAG6596517.1 (Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 903 bits (2333), Expect = 0.0
Identity = 452/553 (81.74%), Postives = 495/553 (89.51%), Query Frame = 0

Query: 5   SINLRLHHHILHLSPSISAHRHRSVVSQVPFSPISPKLLL----KRRSNFTRMASAAGRP 64
           ++N+ LHHH       ++  R    VS + FSPI+PKLLL    K  +NF  MASAA  P
Sbjct: 17  AVNVHLHHHNFRFPACLTTVRRHPTVSLLQFSPITPKLLLRHGFKSNTNFIPMASAADNP 76

Query: 65  TNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGM 124
           T++ H  ++      GS+DLRPTTPEQRTLAWPEMA LW+GL+VGVPAYYLAGSLVDLGM
Sbjct: 77  TDNTHVENS------GSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGM 136

Query: 125 AWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCG 184
           AWWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+ ARSSFGIHGAH+PTLLRALIGCG
Sbjct: 137 AWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCG 196

Query: 185 WYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDG 244
           WYGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLASVWKGMDG
Sbjct: 197 WYGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDG 256

Query: 245 IRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTANISFW 304
           IRKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPSLTANISFW
Sbjct: 257 IRKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFW 316

Query: 305 ATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLL 364
           ATLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +ISNPIDLL
Sbjct: 317 ATLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLL 376

Query: 365 GHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVF 424
           GHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNL+PS F+FTRGA+LTAV+GIVF
Sbjct: 377 GHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALLTAVIGIVF 436

Query: 425 QPGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSGAYYYS 484
           QP RLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY IR+REL VEDLYSSSP+GAYYYS
Sbjct: 437 QPWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSSSPTGAYYYS 496

Query: 485 GGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCYWGLSV 544
           GGFNL A+AALVVGVLPVIPGFLEKV+IVS+VP+VF +IYGNAWFIS+F+AGFCYWGLSV
Sbjct: 497 GGFNLTAVAALVVGVLPVIPGFLEKVKIVSKVPQVFTVIYGNAWFISTFIAGFCYWGLSV 556

Query: 545 LLKRQKVPRPLDP 553
           LLKR+KV   L P
Sbjct: 557 LLKRRKVSSLLGP 563

BLAST of MC05g1339 vs. ExPASy TrEMBL
Match: A0A5D3DPI8 (Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003170 PE=3 SV=1)

HSP 1 Score: 910 bits (2352), Expect = 0.0
Identity = 464/560 (82.86%), Postives = 501/560 (89.46%), Query Frame = 0

Query: 1   MVTQSINLRLHHHILHLSPSISAHRHRSVVSQVP--FSPISPKLLLKRRSN--FTR-MAS 60
           MVT  INL LH HI HL PSIS  R+R  VS  P  FSPI+PKLLLKRRSN  FT  MAS
Sbjct: 1   MVTHCINLHLHRHIFHLPPSISTLRYRPTVSHSPLLFSPITPKLLLKRRSNLRFTSPMAS 60

Query: 61  AAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSL 120
           A   P+ D    D+      G +DLRPTT EQRTL+WPEMA LW+GLIVGVP+YYLAGSL
Sbjct: 61  ADANPSEDTQNDDS------GKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSL 120

Query: 121 VDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRA 180
           VDLGM+WWQGIATVV ANAVT+ AL+L GHPGTKYGISFP+ ARSSFGIHGAH+PTLLRA
Sbjct: 121 VDLGMSWWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRA 180

Query: 181 LIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVW 240
           LIGCGWYGIETWIAGEAIF+LLPESIK+SSFS +LPWLGTSPLAF+CFLAFWVVQLASVW
Sbjct: 181 LIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLASVW 240

Query: 241 KGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTA 300
           KGMDGIRKLET+SAPILVFLT  LLIWS V+AGG GHMLSLTSKLS+SEFWALFFPSLTA
Sbjct: 241 KGMDGIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPSLTA 300

Query: 301 NISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISN 360
           NISFWATLALNIPDFTRYA++QKDQI+GQLGLPIFMGAFTFVGVAVTSSTGVIFG +ISN
Sbjct: 301 NISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISN 360

Query: 361 PIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAV 420
           PIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+LTAV
Sbjct: 361 PIDLLGHIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAV 420

Query: 421 VGIVFQPGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSG 480
           +GI+FQP RLLSSSESFVYTWLVGYSALLGPIGG+M+TDYYLIR +ELSVEDLYS+SP G
Sbjct: 421 IGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTSPRG 480

Query: 481 AYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCY 540
           AYYYSGGFNL A+ ALVVGVLPVIPGFLEKVEIVS+VPKVF +IYG+AWFISSFLAGFCY
Sbjct: 481 AYYYSGGFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAGFCY 540

Query: 541 WGLSVLLKRQKVPRPLDPLL 555
           WGLSVLLKR+K      PLL
Sbjct: 541 WGLSVLLKRRKASLRFGPLL 554

BLAST of MC05g1339 vs. ExPASy TrEMBL
Match: A0A1S3B756 (purine-uracil permease NCS1 OS=Cucumis melo OX=3656 GN=LOC103486539 PE=3 SV=1)

HSP 1 Score: 910 bits (2352), Expect = 0.0
Identity = 464/560 (82.86%), Postives = 501/560 (89.46%), Query Frame = 0

Query: 1   MVTQSINLRLHHHILHLSPSISAHRHRSVVSQVP--FSPISPKLLLKRRSN--FTR-MAS 60
           MVT  INL LH HI HL PSIS  R+R  VS  P  FSPI+PKLLLKRRSN  FT  MAS
Sbjct: 1   MVTHCINLHLHRHIFHLPPSISTLRYRPTVSHSPLLFSPITPKLLLKRRSNLRFTSPMAS 60

Query: 61  AAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSL 120
           A   P+ D    D+      G +DLRPTT EQRTL+WPEMA LW+GLIVGVP+YYLAGSL
Sbjct: 61  ADANPSEDTQNDDS------GKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSL 120

Query: 121 VDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRA 180
           VDLGM+WWQGIATVV ANAVT+ AL+L GHPGTKYGISFP+ ARSSFGIHGAH+PTLLRA
Sbjct: 121 VDLGMSWWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRA 180

Query: 181 LIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVW 240
           LIGCGWYGIETWIAGEAIF+LLPESIK+SSFS +LPWLGTSPLAF+CFLAFWVVQLASVW
Sbjct: 181 LIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLASVW 240

Query: 241 KGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTA 300
           KGMDGIRKLET+SAPILVFLT  LLIWS V+AGG GHMLSLTSKLS+SEFWALFFPSLTA
Sbjct: 241 KGMDGIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPSLTA 300

Query: 301 NISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISN 360
           NISFWATLALNIPDFTRYA++QKDQI+GQLGLPIFMGAFTFVGVAVTSSTGVIFG +ISN
Sbjct: 301 NISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISN 360

Query: 361 PIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAV 420
           PIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+LTAV
Sbjct: 361 PIDLLGHIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAV 420

Query: 421 VGIVFQPGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSG 480
           +GI+FQP RLLSSSESFVYTWLVGYSALLGPIGG+M+TDYYLIR +ELSVEDLYS+SP G
Sbjct: 421 IGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTSPRG 480

Query: 481 AYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCY 540
           AYYYSGGFNL A+ ALVVGVLPVIPGFLEKVEIVS+VPKVF +IYG+AWFISSFLAGFCY
Sbjct: 481 AYYYSGGFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAGFCY 540

Query: 541 WGLSVLLKRQKVPRPLDPLL 555
           WGLSVLLKR+K      PLL
Sbjct: 541 WGLSVLLKRRKASLRFGPLL 554

BLAST of MC05g1339 vs. ExPASy TrEMBL
Match: A0A6J1KWR9 (purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=3 SV=1)

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 454/552 (82.25%), Postives = 494/552 (89.49%), Query Frame = 0

Query: 6   INLRLHHHILHLSPSISAHRHRSVVSQVPFSPISPKLLLKRR----SNFTRMASAAGRPT 65
           +NL LH H     P ++  R    VS + FSPI+PKLLL+ R    +NF  MASAA  PT
Sbjct: 18  VNLHLHRHNFRFPPCLTTVRRHPTVSLLRFSPITPKLLLRHRFKSNTNFIPMASAANNPT 77

Query: 66  NDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMA 125
           ++ H  +       GS+DLRPTTPEQRTLAWPEMA LW+GL+VGVPAYYLAGSLVDLGMA
Sbjct: 78  DNTHVENP------GSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMA 137

Query: 126 WWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGW 185
           WWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+ ARSSFGIHGAH+PTLLRALIGCGW
Sbjct: 138 WWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 197

Query: 186 YGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDGI 245
           YGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLASVWKGMDGI
Sbjct: 198 YGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 257

Query: 246 RKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTANISFWA 305
           RKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPSLTANISFWA
Sbjct: 258 RKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWA 317

Query: 306 TLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLLG 365
           TLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +ISNPIDLLG
Sbjct: 318 TLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLG 377

Query: 366 HIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQ 425
           HIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+LTAV+GIVFQ
Sbjct: 378 HIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQ 437

Query: 426 PGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSGAYYYSG 485
           P RLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY +R+REL VEDLYSSSP+GAYYYSG
Sbjct: 438 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFLRKRELIVEDLYSSSPTGAYYYSG 497

Query: 486 GFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCYWGLSVL 545
           GFNL A+AALVVGVLPVIPGFLEKV+IVS+VP+VF +IYGNAWFISSF+AGFCYWGLSVL
Sbjct: 498 GFNLTAVAALVVGVLPVIPGFLEKVKIVSKVPEVFTVIYGNAWFISSFIAGFCYWGLSVL 557

Query: 546 LKRQKVPRPLDP 553
           LKR+KV   L P
Sbjct: 558 LKRRKVSSCLGP 563

BLAST of MC05g1339 vs. ExPASy TrEMBL
Match: A0A6J1GDF7 (purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 PE=3 SV=1)

HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 448/553 (81.01%), Postives = 494/553 (89.33%), Query Frame = 0

Query: 5   SINLRLHHHILHLSPSISAHRHRSVVSQVPFSPISPKLLLKRR----SNFTRMASAAGRP 64
           ++NL LHHH       ++  R    VS + FSPI+PKLLL+ R    +NF  MASAA  P
Sbjct: 17  AVNLHLHHHNFRFPACLTTVRRHPTVSLLQFSPITPKLLLRHRFNSNTNFIPMASAADNP 76

Query: 65  TNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGM 124
           T++ H  ++      GS+DLRPT PEQRTLAWPEMA LW+GL+VGVPAYYLAGSLVDLGM
Sbjct: 77  TDNTHVENS------GSDDLRPTMPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGM 136

Query: 125 AWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCG 184
           AWWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+ ARSSFGIHGAH+PTLLRALIGCG
Sbjct: 137 AWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCG 196

Query: 185 WYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDG 244
           WYGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLASVWKGMDG
Sbjct: 197 WYGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDG 256

Query: 245 IRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTANISFW 304
           IRKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPSLTANISFW
Sbjct: 257 IRKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFW 316

Query: 305 ATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLL 364
           ATLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +ISNPIDLL
Sbjct: 317 ATLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLL 376

Query: 365 GHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVF 424
           GHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNL+PS F+FTRGA+LTAV+GIVF
Sbjct: 377 GHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALLTAVIGIVF 436

Query: 425 QPGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSGAYYYS 484
           QP RLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY IR+REL VEDLYSSSP+GAYY+S
Sbjct: 437 QPWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSSSPTGAYYFS 496

Query: 485 GGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCYWGLSV 544
           GGFNL A+AALVVGVLPVIPGFLEKV++VS+VP++F +IYGNAWFIS+F+AGFCY GLSV
Sbjct: 497 GGFNLTAVAALVVGVLPVIPGFLEKVKLVSKVPQLFTVIYGNAWFISTFIAGFCYLGLSV 556

Query: 545 LLKRQKVPRPLDP 553
           LLKR+KV   L P
Sbjct: 557 LLKRRKVSSWLGP 563

BLAST of MC05g1339 vs. ExPASy TrEMBL
Match: A0A0A0LAP3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G746620 PE=3 SV=1)

HSP 1 Score: 893 bits (2308), Expect = 0.0
Identity = 452/558 (81.00%), Postives = 493/558 (88.35%), Query Frame = 0

Query: 1   MVTQSINLRLHHHILHLSPSISAHRHRSVVSQ--VPFSPISPKLLLKRRSN--FTR-MAS 60
           MVT  INL LH HI HL PS S  R+R   S   +PFSPI+PKLLLKRRSN  FT  MAS
Sbjct: 1   MVTHCINLHLHRHIFHLPPSFSTLRYRPTASPSPLPFSPITPKLLLKRRSNLQFTSPMAS 60

Query: 61  AAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSL 120
           A   P  D    D+      G EDLRPTT EQRTL+WPEMA LW+GLIVGVP+YYLAGSL
Sbjct: 61  ATANPPEDTQNEDS------GKEDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSL 120

Query: 121 VDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRA 180
           VDLGM+WWQGIATVVAANAVT+ AL+L GHPGTKYGISFP+ ARSSFGIHGAH+PTLLRA
Sbjct: 121 VDLGMSWWQGIATVVAANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRA 180

Query: 181 LIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVW 240
           LIGCGWYGIETWIAGEAI +LLP+SIK+S+FS  LPWLGTSPLAF+CFL FWV+QLASVW
Sbjct: 181 LIGCGWYGIETWIAGEAISLLLPQSIKKSAFSQTLPWLGTSPLAFTCFLGFWVIQLASVW 240

Query: 241 KGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTA 300
           KGMDGIRKLET+SAPILVFLT  LLIWS V+AGG GHMLSLTSKLS SEFWALFFPSLTA
Sbjct: 241 KGMDGIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSFSEFWALFFPSLTA 300

Query: 301 NISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISN 360
           NISFWATLALNIPDFTRYA++QKDQI+GQLGLPIFMGAFTFVGVAVTSSTGVIFG +ISN
Sbjct: 301 NISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISN 360

Query: 361 PIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAV 420
           PIDLL HIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+LTAV
Sbjct: 361 PIDLLDHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAV 420

Query: 421 VGIVFQPGRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRQRELSVEDLYSSSPSG 480
           +GI+FQP RLLSSSESFVYTWLVGYSALLGPIGG+M+TDYYLIR +ELSVEDLYS+SP+ 
Sbjct: 421 IGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMMTDYYLIRGKELSVEDLYSTSPTA 480

Query: 481 AYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCY 540
           AYYYSGGFNL A+ ALVVGVLPVIPGFLEKVE+VS+VPKVF +IYG+AWF+S+FLAGFCY
Sbjct: 481 AYYYSGGFNLVAVVALVVGVLPVIPGFLEKVELVSKVPKVFSVIYGSAWFVSTFLAGFCY 540

Query: 541 WGLSVLLKRQKVPRPLDP 553
           WGLSVLLKR+K      P
Sbjct: 541 WGLSVLLKRRKASLRFGP 552

BLAST of MC05g1339 vs. TAIR 10
Match: AT5G03555.1 (permease, cytosine/purines, uracil, thiamine, allantoin family protein )

HSP 1 Score: 706.1 bits (1821), Expect = 2.3e-203
Identity = 346/515 (67.18%), Postives = 421/515 (81.75%), Query Frame = 0

Query: 35  FSPISP--KLLLKRRSNF-TRMASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLA 94
           FSPI P   L+L R      R+++  G   ND  + ++   P+L ++DL+PTTP QRT +
Sbjct: 74  FSPILPGDNLVLSRSGVIRPRLSAMTGSEINDHGYDESQFDPSLTNDDLKPTTPSQRTFS 133

Query: 95  WPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYG 154
           W +M++LW+GL+VGVP YYLAGSLVDLGMAWWQGIATVV AN + +  LVLT  PGT YG
Sbjct: 134 WLDMSSLWIGLVVGVPTYYLAGSLVDLGMAWWQGIATVVTANLILLVPLVLTAQPGTLYG 193

Query: 155 ISFPILARSSFGIHGAHVPTLLRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLP 214
           ISFP+LARSSFGI GAH+PTLLRAL+GCGWYGIETWI GEAIF+LLP  IK+S+ SH LP
Sbjct: 194 ISFPVLARSSFGIRGAHIPTLLRALVGCGWYGIETWIGGEAIFLLLPGHIKKSALSHTLP 253

Query: 215 WLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLG 274
           WLGTSPL FSCF+ FW+ QL  VW+GMDGIRKLE YSAPIL+ LT  LL WSY++AGG G
Sbjct: 254 WLGTSPLEFSCFIVFWLAQLCIVWRGMDGIRKLEKYSAPILISLTSCLLAWSYLKAGGFG 313

Query: 275 HMLSLTSKLSASEFWALFFPSLTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFM 334
           HMLSL+SKL++++FW LFFPSLTANISFWATLALNIPDF+R+A++Q DQI+GQ+GLP+FM
Sbjct: 314 HMLSLSSKLTSAQFWTLFFPSLTANISFWATLALNIPDFSRFAKSQTDQIIGQVGLPVFM 373

Query: 335 GAFTFVGVAVTSSTGVIFGRVISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVA 394
           G FTFVGVAVTSST +IFGRVISNPI+LLG IGG  T +LAI GI LAT+TTN+AANVVA
Sbjct: 374 GLFTFVGVAVTSSTSIIFGRVISNPIELLGQIGGLATTLLAIVGISLATLTTNIAANVVA 433

Query: 395 PANALVNLSPSVFSFTRGAVLTAVVGIVFQPGRLLSSSESFVYTWLVGYSALLGPIGGIM 454
           PANALVNL+P  F+F RGA LTAV+GIVFQP RLL SSESFVYTWL+GYSALLGPIGGI+
Sbjct: 434 PANALVNLNPKFFTFGRGAFLTAVLGIVFQPWRLLKSSESFVYTWLIGYSALLGPIGGII 493

Query: 455 LTDYYLIRQRELSVEDLYSSSPSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSR 514
           L DYYLI++ +L++ DLYS SPSG YY+S G+N+AA+ ALV G++PV+PGFL K+  +S+
Sbjct: 494 LVDYYLIKKMKLNIGDLYSLSPSGEYYFSKGYNVAAVVALVAGIIPVVPGFLHKISALSK 553

Query: 515 VPKVFGIIYGNAWFISSFLAGFCYWGLSVLLKRQK 547
           +   F ++Y NA F S  +AGF YW +   L R++
Sbjct: 554 ISNGFVVVYDNALFFSFIIAGFVYWIIMSRLGRKQ 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZD03.2e-20267.18Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1[more]
P945751.7e-7837.14Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI ... [more]
O140351.2e-5834.07Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / A... [more]
Q102793.7e-5731.62Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812... [more]
P757124.3e-4527.17Putative allantoin permease OS=Escherichia coli (strain K12) OX=83333 GN=ybbW PE... [more]
Match NameE-valueIdentityDescription
XP_038906223.10.083.69purine-uracil permease NCS1 [Benincasa hispida][more]
XP_008442760.10.082.86PREDICTED: purine-uracil permease NCS1 [Cucumis melo] >KAA0043951.1 purine-uraci... [more]
XP_023539092.10.082.61purine-uracil permease NCS1 [Cucurbita pepo subsp. pepo][more]
XP_023005190.10.082.25purine-uracil permease NCS1-like [Cucurbita maxima][more]
KAG6596517.10.081.74Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A5D3DPI80.082.86Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3B7560.082.86purine-uracil permease NCS1 OS=Cucumis melo OX=3656 GN=LOC103486539 PE=3 SV=1[more]
A0A6J1KWR90.082.25purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=... [more]
A0A6J1GDF70.081.01purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 P... [more]
A0A0A0LAP30.081.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G746620 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G03555.12.3e-20367.18permease, cytosine/purines, uracil, thiamine, allantoin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001248Purine-cytosine permeasePFAMPF02133Transp_cyt_purcoord: 83..519
e-value: 1.9E-100
score: 336.8
NoneNo IPR availableGENE3D1.10.4160.10Hydantoin permeasecoord: 67..532
e-value: 1.6E-114
score: 384.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 55..84
NoneNo IPR availablePANTHERPTHR30618:SF0PURINE-URACIL PERMEASE NCS1coord: 60..545
NoneNo IPR availablePANTHERPTHR30618NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTERcoord: 60..545
NoneNo IPR availableCDDcd11485SLC-NCS1sbd_YbbW-likecoord: 73..539
e-value: 0.0
score: 523.285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g1339.1MC05g1339.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015851 nucleobase transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015205 nucleobase transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity