MC05g1323 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g1323 vs. ExPASy Swiss-Prot
Match: Q9SA91 (Protein PSK SIMULATOR 2 OS=Arabidopsis thaliana OX=3702 GN=PSI2 PE=2 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 1.2e-14
Identity = 55/153 (35.95%), Postives = 83/153 (54.25%), Query Frame = 0

Query: 345 LAGAGLSIHYAKIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPP 404
           L  AGLS+HYA ++  I+  AS P  + ++ RD L+N LPAT++TALR RL++  +    
Sbjct: 355 LGEAGLSLHYANLIQQIDNIASRPSSLPSNVRDTLYNALPATVKTALRPRLQTLDQEEEL 414

Query: 405 SLYDPVVAAEWKSAVAKILQWLAPMAHDT-----------ETWDSEQSFEKPLAGAGAGE 464
           S+       E K+ + K LQWL P A +T           E  +S   F K   G G GE
Sbjct: 415 SV------PEIKAEMEKSLQWLVPFAENTTKAHQGFGWVGEWANSRIEFGK---GKGKGE 474

Query: 465 SRSHVLLLQTLHYADREKTEGAIVQLLVALSNI 487
           +  +   LQTLH+AD+   +  +++L+V L  +
Sbjct: 475 NNGNPTRLQTLHHADKPIVDSYVLELVVWLHRL 498

BLAST of MC05g1323 vs. ExPASy Swiss-Prot
Match: Q9XID5 (Protein PSK SIMULATOR 1 OS=Arabidopsis thaliana OX=3702 GN=PSI1 PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 8.4e-13
Identity = 51/148 (34.46%), Postives = 82/148 (55.41%), Query Frame = 0

Query: 345 LAGAGLSIHYAKIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPP 404
           L  AGL++HYA I+  I+   S    + A  RD L+  LP +I++ALRSR++S       
Sbjct: 380 LGSAGLALHYANIITQIDTLVSRSSTMPASTRDALYQGLPPSIKSALRSRIQSF------ 439

Query: 405 SLYDPVVAAEWKSAVAKILQWLAPMAHDTETWDSEQSF----EKPLAGAGAGE--SRSHV 464
            + + +   + K+ + K LQWL P+A  T T  +   F    E   +G+ A +  +   +
Sbjct: 440 QVKEELTVPQIKAEMEKTLQWLVPVA--TNTTKAHHGFGWVGEWASSGSEANQRPAGQTI 499

Query: 465 LLLQTLHYADREKTEGAIVQLLVALSNI 487
           L + TLH+AD+EKTE  I+ L+V L ++
Sbjct: 500 LRIDTLHHADKEKTEAYILDLVVWLHHL 519

BLAST of MC05g1323 vs. ExPASy Swiss-Prot
Match: P0DO24 (Protein PSK SIMULATOR 3 OS=Arabidopsis thaliana OX=3702 GN=PSI3 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.7e-08
Identity = 41/146 (28.08%), Postives = 75/146 (51.37%), Query Frame = 0

Query: 345 LAGAGLSIHYAKIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPP 404
           L  AGL++HYA I++ I+   +    I ++ RD L+  LP  I+ ALRS+++S       
Sbjct: 372 LGPAGLALHYANIIVQIDTLVARASSITSNARDSLYQSLPPGIKLALRSKIKSF------ 431

Query: 405 SLYDPVVAAEWKSAVAKILQWLAPMAHDTETWDSEQSFEKPLAGAG----AGESRSHVLL 464
           ++   +   + K  + + L WL P+A +T        +    A  G    +  S   +L 
Sbjct: 432 NVDKELSVTQIKDEMERTLHWLVPVAGNTTKAHHGFGWVGEWANTGTDFTSKPSGGDILR 491

Query: 465 LQTLHYADREKTEGAIVQLLVALSNI 487
           ++TL++A +EKTE  I+  ++ L ++
Sbjct: 492 IETLYHASKEKTEIYILGQIIWLQHL 511

BLAST of MC05g1323 vs. NCBI nr
Match: XP_022144636.1 (uncharacterized protein LOC111014277 [Momordica charantia])

HSP 1 Score: 952 bits (2462), Expect = 0.0
Identity = 483/486 (99.38%), Postives = 486/486 (100.00%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL
Sbjct: 26  ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 85

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
           NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM
Sbjct: 86  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 145

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           +RFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV
Sbjct: 146 ERFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 205

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 240
           TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM
Sbjct: 206 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 265

Query: 241 FGLKNFLRLPSEPPLMKLADLKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGRRIR 300
           FGLKNFLRLPSEPPLMKLAD+KSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGRRIR
Sbjct: 266 FGLKNFLRLPSEPPLMKLADVKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGRRIR 325

Query: 301 KTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYAKIVIL 360
           KTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYAKIVIL
Sbjct: 326 KTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYAKIVIL 385

Query: 361 IEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEWKSAVA 420
           IEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEWKSAVA
Sbjct: 386 IEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEWKSAVA 445

Query: 421 KILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREKTEGAIVQLL 480
           KILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREKTEGAIV+LL
Sbjct: 446 KILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREKTEGAIVELL 505

Query: 481 VALSNI 486
           VALSNI
Sbjct: 506 VALSNI 511

BLAST of MC05g1323 vs. NCBI nr
Match: XP_038904412.1 (protein PSK SIMULATOR 2-like [Benincasa hispida])

HSP 1 Score: 601 bits (1550), Expect = 7.71e-210
Identity = 327/497 (65.79%), Postives = 381/497 (76.66%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           E + IGFL+ EI+ LMSKLVQLWN+LED EF+R K+ +SNS GI+KLIS D+ FLTELF+
Sbjct: 26  EPSAIGFLSLEITALMSKLVQLWNRLEDNEFSRAKQNLSNSIGIRKLISTDEAFLTELFM 85

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
            EIV DLQY+A+SI RF  KCSDPVLHEFE+FV+DP+KN F+WFGWQY+WKKM+RRVKKM
Sbjct: 86  KEIVEDLQYIAKSIVRFGAKCSDPVLHEFERFVKDPLKNEFNWFGWQYKWKKMDRRVKKM 145

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           QRFV LTAEL REMEILAEVERN+KRTT IF+F GG G+SFK+RKK+ WHRR VQSLKL+
Sbjct: 146 QRFVVLTAELWREMEILAEVERNLKRTTTIFSFSGGQGKSFKYRKKISWHRRRVQSLKLI 205

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLA---SGEFN 240
           TPWNR FDYI RLFMRS+IT++ERIKIVFGV+E+RRP     EKSA   RL    S  ++
Sbjct: 206 TPWNRTFDYIFRLFMRSMITIIERIKIVFGVREMRRPEE-SGEKSA--DRLVPAVSYRWS 265

Query: 241 EMMFGLKNFLRLPSEPPLMKLADLKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGR 300
           E+    K        PPLMK++       SE K+  QF HFRSF+DCK   I S      
Sbjct: 266 ELEEQGKK-QNYNHSPPLMKIS-------SESKRFTQFPHFRSFRDCKNREIGSS-QPSL 325

Query: 301 RIRKTTSLNLKNR-VENRASS-PSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYA 360
            +RKT+SL +KNR V++RASS P R NGG+YSISSFF  ENLS  P +SL  A L+IHY 
Sbjct: 326 PVRKTSSLKVKNRAVDSRASSSPKRTNGGHYSISSFFIKENLSNSPKNSLGAAALAIHYG 385

Query: 361 KIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEW 420
           KIV+ IE  ASAPHLIG+++RD+LFNMLP +I  ALR RLR   K R  SLYDPV+AAE 
Sbjct: 386 KIVVSIENLASAPHLIGSEERDDLFNMLPVSIVKALRLRLRKTTKARHSSLYDPVLAAES 445

Query: 421 KSAVAKILQWLAPMAHDTETWDSEQSFEKP------LAGAGAGESRSHVLLLQTLHYADR 480
           KSA+AKILQWLAPMAHD  TW SEQSFEK         G G+G SRSHVLLLQTLHYADR
Sbjct: 446 KSAMAKILQWLAPMAHDMNTWHSEQSFEKQPDCEGNSDGGGSG-SRSHVLLLQTLHYADR 505

Query: 481 EKTEGAIVQLLVALSNI 486
           EKTE AIV+LLVALSNI
Sbjct: 506 EKTEDAIVELLVALSNI 509

BLAST of MC05g1323 vs. NCBI nr
Match: XP_023528100.1 (uncharacterized protein LOC111791118 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 600 bits (1546), Expect = 2.91e-209
Identity = 322/491 (65.58%), Postives = 371/491 (75.56%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           + AGIGFL+FEI+ LMSKLVQ+WN+LED EF RVK+QISNS GI  LIS+D++FL EL +
Sbjct: 26  DMAGIGFLSFEIAPLMSKLVQVWNRLEDEEFMRVKQQISNSKGIGILISNDEQFLMELLM 85

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
            EIV DLQY+A+SIARF  KCSDPVLHEFEKFV DP+KN FDW  WQYRW+KMERRVKKM
Sbjct: 86  VEIVGDLQYIAKSIARFGDKCSDPVLHEFEKFVEDPMKNEFDWGRWQYRWEKMERRVKKM 145

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           Q+F+ LTAELSREME+LA VERN+ R T +F+F GGGG+SF +RK++ WHRR VQSLKL+
Sbjct: 146 QKFIKLTAELSREMEVLAAVERNLGRNTTVFSFAGGGGKSFNYRKEISWHRRRVQSLKLL 205

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 240
           TPWNR FDYILRLFMRSIIT+ +RIKIVFGV E+R P   +S K   G R+ +       
Sbjct: 206 TPWNRTFDYILRLFMRSIITITQRIKIVFGVNEMRLPE--DSGKRFTGRRIPADNCRRSK 265

Query: 241 F---GLKNFLRLPSEPPLMKLADLKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGR 300
               G K        PPLMK +       +E K+  QF HFRSF+DCK G   SPP   +
Sbjct: 266 VEEQGKKQSYN--RSPPLMKNS-------AESKRFSQFPHFRSFRDCKYGATVSPP-PSQ 325

Query: 301 RIRKTTSLNLKNRV-ENRASS-PSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYA 360
            +RKT+SL L N   ENRASS P RINGG+YSISSFF  ENL  PPP+SL  A LSIHY 
Sbjct: 326 PVRKTSSLKLTNTASENRASSSPRRINGGHYSISSFFIKENLPNPPPNSLGAAALSIHYG 385

Query: 361 KIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEW 420
           KIV LIE+ ASAP LI   +RD+L+NMLP +IR ALRSRLR AAK+R  SLYDPV+AAEW
Sbjct: 386 KIVTLIEQMASAPRLIDNKERDKLYNMLPLSIRKALRSRLRKAAKIRHSSLYDPVLAAEW 445

Query: 421 KSAVAKILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREKTEGA 480
           KSA AKILQWL  MAHD ETW SE SFEK   G   G SR  VLLLQTLHYADREKTEGA
Sbjct: 446 KSATAKILQWLTSMAHDMETWHSEHSFEKKPNGGDMGGSRPRVLLLQTLHYADREKTEGA 504

Query: 481 IVQLLVALSNI 486
           IV++LVALSNI
Sbjct: 506 IVEVLVALSNI 504

BLAST of MC05g1323 vs. NCBI nr
Match: KAG7028067.1 (hypothetical protein SDJN02_09247, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 597 bits (1538), Expect = 1.29e-208
Identity = 320/491 (65.17%), Postives = 376/491 (76.58%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           ++A IGFL+FEI+ LMSKLV LWN+L+D+EFT VK+ +S+STGIKKLISDD++FL ELF+
Sbjct: 23  DTAAIGFLSFEITALMSKLVLLWNRLQDSEFTTVKQNLSSSTGIKKLISDDEQFLMELFM 82

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
            EIV DLQY+A+SI RF  KCSDPVLHEFEKFV DP+KN F+WFGWQY+WKKM+RRVKKM
Sbjct: 83  KEIVEDLQYIAKSIVRFGPKCSDPVLHEFEKFVTDPLKNEFNWFGWQYKWKKMDRRVKKM 142

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           QRF+ L AEL+REMEI+AE+ER+++R TAIFAF GG   SFK+RKK+ WHRRHVQSLKL 
Sbjct: 143 QRFIVLAAELTREMEIVAEIERHLRRKTAIFAFAGGKRHSFKYRKKISWHRRHVQSLKLT 202

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 240
           TPWNR +DYI+RLFMRS+IT++ERIKIVFGVKEIRR      EKSA    +  G      
Sbjct: 203 TPWNRTYDYIVRLFMRSMITIIERIKIVFGVKEIRRL-DYGGEKSA-DRLVPVGSCRRSE 262

Query: 241 FGLKNFLRLPSE-PPLMKLADLKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGRRI 300
              +   +  +  PPLMK++       SE KK  QF+HF SF+DCK GGI SPP      
Sbjct: 263 LEEQGKKQSNNHCPPLMKIS-------SEIKKAPQFSHFPSFRDCKDGGIGSPP-PLTAA 322

Query: 301 RKTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYAKIVI 360
           R+T  L LKNR     SSP R NGG+YSISSFF  ENLS PPP+SL  A L+IHY+KIVI
Sbjct: 323 RQTLPLTLKNRAP---SSPRRTNGGHYSISSFFIKENLSNPPPNSLGAAALAIHYSKIVI 382

Query: 361 LIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEWKSAV 420
            IE+ ASAPHLI A+DR ELFNMLPA+IR A+R+    AAK R P LY+P +AAEWKSA+
Sbjct: 383 SIERLASAPHLITAEDRHELFNMLPASIRNAVRT----AAKFRHPPLYNPDLAAEWKSAM 442

Query: 421 AKILQWLAPMAHDTETWDSEQSFEKPLAGAGA----GESRSHVLLLQTLHYADREKTEGA 480
            +I QWLAPMA D ETW SEQS EK   GAG     G SRSHVLLLQT HYADREKTEGA
Sbjct: 443 GRIFQWLAPMARDMETWHSEQSIEKQPDGAGTAADGGGSRSHVLLLQTFHYADREKTEGA 496

Query: 481 IVQLLVALSNI 486
           IV+LLVALSNI
Sbjct: 503 IVELLVALSNI 496

BLAST of MC05g1323 vs. NCBI nr
Match: KAG6580658.1 (Protein PSK SIMULATOR 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 598 bits (1541), Expect = 1.67e-208
Identity = 322/491 (65.58%), Postives = 371/491 (75.56%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           + AGIGFL+FEI+ LMSKLVQLWN+LED EF RVK QISNS GI  LIS+D++FL EL +
Sbjct: 26  DMAGIGFLSFEIAPLMSKLVQLWNRLEDEEFMRVKRQISNSKGIGILISNDEQFLMELLM 85

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
            EIV DLQY+A+SIARF  KCSDPVLHEFEKFV+DP+K+ FDW  WQYRW+KMERRVKKM
Sbjct: 86  VEIVGDLQYIAKSIARFGDKCSDPVLHEFEKFVQDPMKSEFDWGRWQYRWEKMERRVKKM 145

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           Q+F+ LTAELSREME+LA +ERN+ R T IF+F GGG +SF +RK++ WHRR VQSLKL+
Sbjct: 146 QKFIVLTAELSREMEVLAAIERNLGRNTTIFSFAGGGRKSFNYRKEISWHRRRVQSLKLL 205

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 240
           TPWNR FDYILRLFMRSIIT+ +RIKIVFGV EIR P   +S K   G R+ +       
Sbjct: 206 TPWNRTFDYILRLFMRSIITITQRIKIVFGVNEIRLPE--DSGKRFTGRRIPADNCRRSK 265

Query: 241 F---GLKNFLRLPSEPPLMKLADLKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGR 300
               G K        PPLMK +       +E K+  QF HFRSF+DCK G   SPP   +
Sbjct: 266 VEEQGKKQSYN--RSPPLMKNS-------AESKRFSQFPHFRSFRDCKYGATVSPP-PSQ 325

Query: 301 RIRKTTSLNLKNR-VENRASS-PSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYA 360
            +RKT+SL LKN   ENRASS P RINGG+ SISSFF  ENL  PPP+SL  A LSIHY 
Sbjct: 326 PVRKTSSLKLKNTGAENRASSSPRRINGGHKSISSFFIKENLPNPPPNSLGAAALSIHYG 385

Query: 361 KIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEW 420
           KIV LIE+ ASAP LI   +RD+L+NMLP +IR ALRSRLR AAK+R  SLYDPV+AAEW
Sbjct: 386 KIVTLIEQMASAPRLIDNKERDKLYNMLPLSIRKALRSRLRKAAKIRHSSLYDPVLAAEW 445

Query: 421 KSAVAKILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREKTEGA 480
           KSA AKILQWL  MAHD ETW SE SFEK   G   G S+ HVLLLQTLHYADREKTEGA
Sbjct: 446 KSATAKILQWLTSMAHDMETWHSEHSFEKKPDGGDMGGSKPHVLLLQTLHYADREKTEGA 504

Query: 481 IVQLLVALSNI 486
           IV++LVALSNI
Sbjct: 506 IVEVLVALSNI 504

BLAST of MC05g1323 vs. ExPASy TrEMBL
Match: A0A6J1CU02 (uncharacterized protein LOC111014277 OS=Momordica charantia OX=3673 GN=LOC111014277 PE=4 SV=1)

HSP 1 Score: 952 bits (2462), Expect = 0.0
Identity = 483/486 (99.38%), Postives = 486/486 (100.00%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL
Sbjct: 26  ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 85

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
           NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM
Sbjct: 86  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 145

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           +RFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV
Sbjct: 146 ERFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 205

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 240
           TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM
Sbjct: 206 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 265

Query: 241 FGLKNFLRLPSEPPLMKLADLKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGRRIR 300
           FGLKNFLRLPSEPPLMKLAD+KSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGRRIR
Sbjct: 266 FGLKNFLRLPSEPPLMKLADVKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGRRIR 325

Query: 301 KTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYAKIVIL 360
           KTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYAKIVIL
Sbjct: 326 KTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYAKIVIL 385

Query: 361 IEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEWKSAVA 420
           IEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEWKSAVA
Sbjct: 386 IEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEWKSAVA 445

Query: 421 KILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREKTEGAIVQLL 480
           KILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREKTEGAIV+LL
Sbjct: 446 KILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREKTEGAIVELL 505

Query: 481 VALSNI 486
           VALSNI
Sbjct: 506 VALSNI 511

BLAST of MC05g1323 vs. ExPASy TrEMBL
Match: A0A6J1H4W1 (uncharacterized protein LOC111460568 OS=Cucurbita moschata OX=3662 GN=LOC111460568 PE=4 SV=1)

HSP 1 Score: 594 bits (1532), Expect = 5.10e-208
Identity = 319/491 (64.97%), Postives = 375/491 (76.37%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           ++A IGFL+FEI+ LMSKLV LWN+L+D+EFT VK+ +S+STGIKKLISDD++FL ELF+
Sbjct: 23  DTAAIGFLSFEITALMSKLVLLWNRLQDSEFTTVKQNLSSSTGIKKLISDDEQFLMELFM 82

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
            EIV DLQY+A+SI RF  KCSDPVLHEFEKFV DP+KN F+WFGWQY+WKKM+RRVKKM
Sbjct: 83  KEIVEDLQYIAKSIVRFGPKCSDPVLHEFEKFVTDPLKNEFNWFGWQYKWKKMDRRVKKM 142

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           QRF+ LT EL+REMEI+AE+ER+++R TAIFAF GG   SFK+RKK+ WHRRHVQSLKL 
Sbjct: 143 QRFIVLTGELTREMEIVAEIERHLRRKTAIFAFAGGKRHSFKYRKKISWHRRHVQSLKLT 202

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 240
           TPWNR +DYI+RLFMRS+IT++ERIKIVFGVKEIRR      EKSA    +  G      
Sbjct: 203 TPWNRTYDYIVRLFMRSMITIIERIKIVFGVKEIRRL-DYGGEKSA-DRLVPVGSCRRSE 262

Query: 241 FGLKNFLRLPSE-PPLMKLADLKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGRRI 300
              +   +  +  PPLMK++       SE KK  QF+HF  F+DCK GGI SPP      
Sbjct: 263 LEEQGKKQSNNHCPPLMKIS-------SEIKKAPQFSHFPLFRDCKDGGIGSPP-PLTAA 322

Query: 301 RKTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYAKIVI 360
           R+T  L LKNR     SSP R NGG+YSISSFF  ENLS PPP+SL  A L+IHY+KIVI
Sbjct: 323 RQTLPLTLKNRAP---SSPRRTNGGHYSISSFFIKENLSNPPPNSLGAAALAIHYSKIVI 382

Query: 361 LIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEWKSAV 420
            IE+ ASAPHLI A+DR ELFNMLPA+IR A+R+    AAK R P LY+P +AAEWKSA+
Sbjct: 383 SIERLASAPHLITAEDRHELFNMLPASIRKAVRT----AAKFRHPPLYNPDLAAEWKSAM 442

Query: 421 AKILQWLAPMAHDTETWDSEQSFEKPLAGAGA----GESRSHVLLLQTLHYADREKTEGA 480
            +I QWLAPMA D ETW SEQS EK   GAG     G SRSHVLLLQT HYADREKTEGA
Sbjct: 443 GRIFQWLAPMARDMETWHSEQSIEKQPDGAGTAADGGGSRSHVLLLQTFHYADREKTEGA 496

Query: 481 IVQLLVALSNI 486
           IV+LLVALSNI
Sbjct: 503 IVELLVALSNI 496

BLAST of MC05g1323 vs. ExPASy TrEMBL
Match: A0A6J1J7K1 (uncharacterized protein LOC111481975 OS=Cucurbita maxima OX=3661 GN=LOC111481975 PE=4 SV=1)

HSP 1 Score: 590 bits (1520), Expect = 1.24e-205
Identity = 318/491 (64.77%), Postives = 370/491 (75.36%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           + AGIGFL+FEI+ LMSKLVQLWN+LED EF RVK+QISNS GI+ LIS+D++FL EL  
Sbjct: 26  DMAGIGFLSFEIAPLMSKLVQLWNRLEDEEFMRVKQQISNSKGIEILISNDEQFLMELLR 85

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
            EIV DLQY+A+SIARF  KCSDPVLH+FEKFV DP+KN FDW  WQYRW+KMERRVKKM
Sbjct: 86  VEIVGDLQYIAKSIARFGDKCSDPVLHKFEKFVEDPMKNEFDWGRWQYRWEKMERRVKKM 145

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           Q+F+  TAELSREME+LA VERN+ R T  F+F GGGG+SF +RK++ WHRR VQSLKL+
Sbjct: 146 QKFIVFTAELSREMEVLAAVERNLGRNTTNFSFAGGGGKSFNYRKEISWHRRRVQSLKLL 205

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 240
           TPWNR FDYILRLFMRSIIT+ +RIKIVFGV E+  P   +S K   G R+ +       
Sbjct: 206 TPWNRTFDYILRLFMRSIITITQRIKIVFGVNEMWLPE--DSGKKITGRRIPADNCRRSK 265

Query: 241 F---GLKNFLRLPSEPPLMKLADLKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPHDGR 300
               G K        PPLMK +       +E K+  QF HFRSF+DCK G   SPP   +
Sbjct: 266 VEEQGKKQSYN--RSPPLMKNS-------AESKRFSQFPHFRSFRDCKYGATVSPP-PSQ 325

Query: 301 RIRKTTSLNLKNR-VENRASSP-SRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYA 360
           ++RKT+SL LKN  VENR SS   RINGG+YSISSFF  ENL + PP+SL  A LSIHY 
Sbjct: 326 QVRKTSSLKLKNTAVENRVSSSLRRINGGHYSISSFFIKENLPSSPPNSLGAAALSIHYG 385

Query: 361 KIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEW 420
           KIV LIE+ ASAP LIG+ +RD+L+NMLP +IR ALRSRLR AAK+R  SLYDPV+AAEW
Sbjct: 386 KIVTLIEQMASAPRLIGSKERDKLYNMLPLSIRKALRSRLRKAAKIRHSSLYDPVLAAEW 445

Query: 421 KSAVAKILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREKTEGA 480
           KSA AKILQWL  MAHD ETW SE S EK   G   G S+  VLLLQTLHYADREKTEGA
Sbjct: 446 KSATAKILQWLTSMAHDMETWHSEHSVEKKPNGGDMGGSKPCVLLLQTLHYADREKTEGA 504

Query: 481 IVQLLVALSNI 486
           IV++LVALSNI
Sbjct: 506 IVEVLVALSNI 504

BLAST of MC05g1323 vs. ExPASy TrEMBL
Match: A0A6J1KWH4 (uncharacterized protein LOC111498831 OS=Cucurbita maxima OX=3661 GN=LOC111498831 PE=4 SV=1)

HSP 1 Score: 588 bits (1515), Expect = 1.88e-205
Identity = 320/492 (65.04%), Postives = 372/492 (75.61%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           ++A IGFL+FEI+ LMSKLV LWN+L+D+EFT VK+ +S+STGIKKLISDD++FL E+F+
Sbjct: 23  DTAAIGFLSFEITALMSKLVLLWNRLQDSEFTTVKQNLSSSTGIKKLISDDEQFLMEIFM 82

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
            EIV DLQY+A+SI RF  KCSDPVLHEFEKFV DP+ N F+WFGWQY+WKKM+RRVKKM
Sbjct: 83  KEIVEDLQYIAKSIVRFGPKCSDPVLHEFEKFVTDPLNNEFNWFGWQYKWKKMDRRVKKM 142

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           QRF+ LTAEL+REMEI+AE+ER+++R TAIFAF GG   SFK+RKK+ WHRRHVQSLKL 
Sbjct: 143 QRFIVLTAELTREMEIVAEIERHLRRKTAIFAFSGGKRHSFKYRKKISWHRRHVQSLKLT 202

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 240
           TPWNR +DYI+RLFMRS+IT++ERIKIVFGVKEIRR      EKSA    +  G      
Sbjct: 203 TPWNRTYDYIVRLFMRSMITIIERIKIVFGVKEIRRL-DYGGEKSA-DRLVPVGSCRRSE 262

Query: 241 FGLKNFLRLPSE-PPLMKLADLKSSVISEG-KKVGQFTHFRSFKDCKGGGIDSPPHDGRR 300
              +   +  +  PPLMK        IS G KK  QF+HF SF+DCK GGI SPP     
Sbjct: 263 LEEQGKKQSNNHCPPLMK--------ISSGIKKAPQFSHFPSFRDCKDGGIGSPP-PLTA 322

Query: 301 IRKTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSIHYAKIV 360
            R+T  L +KNR     SSP R NGG+YSISSFF  ENLS PPP+SL  A L+IHY+KIV
Sbjct: 323 ARQTLPLTVKNRAP---SSPRRTNGGHYSISSFFIKENLSNPPPNSLGVAALAIHYSKIV 382

Query: 361 ILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVAAEWKSA 420
           ILIE+ ASAPHLI A DR ELFNMLPA+IR AL S    AAK R P LY P +AAEWKSA
Sbjct: 383 ILIERLASAPHLITAKDRHELFNMLPASIRKALMS----AAKFRHPPLYYPDLAAEWKSA 442

Query: 421 VAKILQWLAPMAHDTETWDSEQSFEKPLAGAGA----GESRSHVLLLQTLHYADREKTEG 480
           + +I QWLAPMA D ETW SEQS EK   GAG     G SRSHVLLLQT HYADREKTE 
Sbjct: 443 MGRIFQWLAPMARDMETWHSEQSIEKQPDGAGTAADGGGSRSHVLLLQTFHYADREKTES 496

Query: 481 AIVQLLVALSNI 486
           AIV+LLVALSNI
Sbjct: 503 AIVELLVALSNI 496

BLAST of MC05g1323 vs. ExPASy TrEMBL
Match: A0A6J1F859 (uncharacterized protein LOC111441752 OS=Cucurbita moschata OX=3662 GN=LOC111441752 PE=4 SV=1)

HSP 1 Score: 588 bits (1517), Expect = 4.22e-205
Identity = 319/494 (64.57%), Postives = 371/494 (75.10%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           + AGIGFL+FEI+ LM KLVQLWN+LED EF RVK QIS+S GI  LIS+D++FL EL +
Sbjct: 26  DMAGIGFLSFEIAPLMWKLVQLWNRLEDEEFMRVKRQISDSKGIGILISNDEQFLMELLM 85

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
            EIV DLQY+A+SIARF  KCSDPVLHEFEKFV+DP+K+ FDW  WQYRW+KMERRVKKM
Sbjct: 86  VEIVGDLQYIAKSIARFGDKCSDPVLHEFEKFVQDPMKSEFDWGRWQYRWEKMERRVKKM 145

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           Q+F+ LTAELSREME+LA +ERN+ R T IF+F GGG +SF +RK++ WHRR VQSLKL+
Sbjct: 146 QKFIVLTAELSREMEVLAAIERNLGRNTTIFSFAGGGRKSFNYRKEISWHRRRVQSLKLL 205

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMM 240
           TPWNR FDYILRLFMRSIIT+ +RIKIVFGV E+R P   +S K   G R+ +       
Sbjct: 206 TPWNRTFDYILRLFMRSIITITQRIKIVFGVNEMRLPE--DSGKRFTGRRIPADNCRRSK 265

Query: 241 F---GLKNFLRLPS---EPPLMKLADLKSSVISEGKKVGQFTHFRSFKDCKGGGIDSPPH 300
               G K   +  S    PPLMK +       +E K+  QF HFRSF+DCK G   SPP 
Sbjct: 266 VEEQGKKQQGKKQSYNRSPPLMKNS-------AESKRFSQFPHFRSFRDCKYGATVSPP- 325

Query: 301 DGRRIRKTTSLNLKNR-VENRASS-PSRINGGNYSISSFFSTENLSTPPPSSLAGAGLSI 360
             + +RKT+SL LKN   ENRASS P RINGG+ SISSFF  ENL  PPP+SL  A LSI
Sbjct: 326 PSQPVRKTSSLKLKNTGTENRASSSPRRINGGHKSISSFFIKENLPNPPPNSLGAAALSI 385

Query: 361 HYAKIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVVA 420
           HY KIV LIE+ ASAP LI   +RD+L+NMLP +IR ALRSRLR AAK+R  SLYDPV+A
Sbjct: 386 HYGKIVTLIEQMASAPRLIDNKERDKLYNMLPLSIRKALRSRLRKAAKIRHSSLYDPVLA 445

Query: 421 AEWKSAVAKILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREKT 480
           AEWKSA AKILQWL  MAHD ETW SE SFEK   G   G S+  VLLLQTLHYADREKT
Sbjct: 446 AEWKSATAKILQWLTSMAHDMETWHSEHSFEKKPDGGDMGGSKPRVLLLQTLHYADREKT 505

Query: 481 EGAIVQLLVALSNI 486
           EGAIV++LVALSNI
Sbjct: 506 EGAIVEVLVALSNI 509

BLAST of MC05g1323 vs. TAIR 10
Match: AT5G04550.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 292.0 bits (746), Expect = 9.0e-79
Identity = 195/558 (34.95%), Postives = 298/558 (53.41%), Query Frame = 0

Query: 1   ESAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFL 60
           + A +G LAFE++ L+SKLV LW  L D    R++++I++STGIKKL+S+DD F+  L  
Sbjct: 22  DKAVLGVLAFEVASLLSKLVHLWQSLSDKNVARLRDEITHSTGIKKLVSEDDDFIVRLIR 81

Query: 61  NEIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKM 120
           +E++ +++ VA+++AR A KC+DP L  FE    D +K   D +GWQ+ WKKM+++ KKM
Sbjct: 82  DEMMENVENVAKAVARLARKCNDPKLKCFENCFSDMMKTGADPYGWQFGWKKMDKKAKKM 141

Query: 121 QRFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRKKVKWHRRHVQSLKLV 180
           +RF++  A L +E EILA++E+  KR   + +         +++KKV W R  V++L+ V
Sbjct: 142 ERFISSNASLYQETEILADLEQTFKR---MKSNESATDNLLEYQKKVTWKRHEVKNLRDV 201

Query: 181 TPWNRGFDYILRLFMRSIITVVERIKIVFG-----------------------VKEIRRP 240
           + WNR +DY + L +RS+ T++ R K VFG                       V  I  P
Sbjct: 202 SLWNRTYDYTVILLVRSVFTILSRTKHVFGISYRVEASDVSSADSDFIGRSHSVSTILTP 261

Query: 241 PSVESEKSAAGHRLASGEFNEM-----------MFGLKNFL--RLPSEPP---------- 300
            S +SE S    R ASG                   + +FL   L +E P          
Sbjct: 262 VSHKSESSGL-PRFASGPLGRFTGPASGSAATRSTKMSDFLSGSLSAESPKSGPLVAEKH 321

Query: 301 ---------LMKLADLKSSVISEG---KKVGQFTHFRSF----KDCKGGGIDS-PPHDGR 360
                    L K+      +I  G   KK+GQ     S     K  K   +    P  G 
Sbjct: 322 KRFKFYSGQLGKITSKSGPLIGMGKNNKKMGQTPERPSISSVKKQLKANRLTQVGPFKGC 381

Query: 361 RIRKTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTP--------PPSSLAGAG 420
            +       L  R  N A + S  +      S+    ENL+ P         P++L  A 
Sbjct: 382 MVSHDGITPLSTRARNGARNSSAEHHILEDNSNSVHVENLTLPSRPKLSDAAPNTLGTAC 441

Query: 421 LSIHYAKIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAK-VRPPSLYD 480
           L++HYA ++I+IE+  ++PHLIG D RD+L+NMLPA++RT+LR RL+  +K +   ++YD
Sbjct: 442 LALHYANVIIVIERFVASPHLIGDDARDDLYNMLPASVRTSLRERLKPYSKNLSSSTVYD 501

Query: 481 PVVAAEWKSAVAKILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYAD 487
           P +A EW  A+A IL+WL P+AH+   W SE+S+E       +  SR+H++L QTL +A+
Sbjct: 502 PGLAREWTDAMAGILEWLGPLAHNMIKWQSERSYEHQ-----SLVSRTHIVLAQTLFFAN 561

BLAST of MC05g1323 vs. TAIR 10
Match: AT3G23160.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 246.5 bits (628), Expect = 4.4e-65
Identity = 169/495 (34.14%), Postives = 260/495 (52.53%), Query Frame = 0

Query: 5   IGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFLNEIV 64
           IG L+FE++ +MSK + L   L DTE +++K ++ +S G++KL+S D+  L +L ++E +
Sbjct: 43  IGILSFEVANVMSKTIHLHRSLSDTEISKLKAEVFHSEGVRKLVSSDENHLLDLSVSEKL 102

Query: 65  RDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKMQRFV 124
            DL  VA  ++R   KC++P L  FE    D V    D+    +  K ME  VKKM+RFV
Sbjct: 103 DDLSRVASVVSRLGKKCNEPALQGFEHVYEDIVNGAIDFRKLGFLVKDMESMVKKMERFV 162

Query: 125 ALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFK-FRKKVKWHRRHVQSLKLVTPW 184
             T  L  EME++ E+E+ I +            ES K F +K+ W R+ V+SL+  + W
Sbjct: 163 NATCSLYCEMEVMNELEQAIVKLQR----SQQHQESVKAFEQKLMWQRQDVKSLRDGSLW 222

Query: 185 NRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRLASGEFNEMMFGL 244
           N+ +D ++ +  R++ T+  RI+ VFG   +R    V  ++  + +  +    +  + G 
Sbjct: 223 NQTYDKVVEMLARTVCTIYGRIETVFGGLGLRGKKDVTLKRDRSKNEASKAVNSRSVAGF 282

Query: 245 KNFLRLPSEPPLMKLADLKSSVISEGKKVGQFTH------FRSFKDCK------GGGIDS 304
           K               D + S   E  + G F         R F +C       G   D 
Sbjct: 283 K---------------DSRRSEADEFTRAGDFNFPCGTNPGRMFMECLAMNRTIGDDDDD 342

Query: 305 PPHDGRRIRKTTSLNLKNRVENRASSPSRINGGNYSISSFFSTENLSTPPPSSLAGAGLS 364
              DG RI    S     R  N+    SR+            T++ S    S++ G+ LS
Sbjct: 343 DDEDGGRITFPLSTARMIR-SNKFGFKSRL------------TQHASA---STIGGSALS 402

Query: 365 IHYAKIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPVV 424
           +HYA +VI++EK    PHLIG + RD+L+ MLP +++T L++ LRS  K    S+YD  +
Sbjct: 403 LHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTSLKTTLKASLRSYLK--NISIYDAPL 462

Query: 425 AAEWKSAVAKILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADREK 484
           A +WK  +  IL WLAP+AH+   W SE++FE+          R++VLLLQTL++ADREK
Sbjct: 463 AHDWKETIDGILSWLAPLAHNMIRWQSERNFEQ----QNQIVKRTNVLLLQTLYFADREK 496

Query: 485 TEGAIVQLLVALSNI 487
           TE AI +LLV L+ I
Sbjct: 523 TEAAICKLLVGLNYI 496

BLAST of MC05g1323 vs. TAIR 10
Match: AT5G51670.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 204.5 bits (519), Expect = 1.9e-52
Identity = 139/496 (28.02%), Postives = 246/496 (49.60%), Query Frame = 0

Query: 2   SAGIGFLAFEISVLMSKLVQLWNQLEDTEFTRVKEQISNSTGIKKLISDDDRFLTELFLN 61
           ++ +G L+FE++ +M+KL+ L + L D+     ++   +  G+ K+++ D+ F   L   
Sbjct: 34  TSSVGVLSFEVARVMTKLLHLTHSLTDSNLLTPRDHSLSLEGLTKIVNGDETFHLSLVCA 93

Query: 62  EIVRDLQYVARSIARFATKCSDPVLHEFEKFVRDPVKNHFDWFGWQYRWKKMERRVKKMQ 121
           E+   L + A S++R + +C+   L  F +   +      D  GW    K  E + KK++
Sbjct: 94  ELADSLAHAANSVSRLSNRCTTASLRSFHRLFHEFADMGRDPHGWVMNCKDTEAKNKKIE 153

Query: 122 RFVALTAELSREMEILAEVERNIKRTTAIFAFGGGGGESFKFRK----------KVKWHR 181
           R+V++T  L REME +A +E ++++ +          E ++ +K          K++  +
Sbjct: 154 RYVSVTTALYREMEEMAILENSLRKQSLQIGIEFEEEEDYENKKDVMKVIDLQNKIERQK 213

Query: 182 RHVQSLKLVTPWNRGFDYILRLFMRSIITVVERIKIVFGVKEIRRPPSVESEKSAAGHRL 241
           +HV+ LK  + WN+ FD ++ +  RS+ T + R+K VF            S  +A G+ +
Sbjct: 214 QHVKYLKDRSLWNKSFDTVVLILARSVFTALARLKSVF------------SSAAATGYMV 273

Query: 242 ASGEFNEMMFGLKNFLRLPSEPPLMKLADLKSSVISEGKKVGQFTHFRSFKDCKGGGIDS 301
            +                        ++ L  S+ S    +                +  
Sbjct: 274 PT-----------------------VVSSLPRSLSSSSSSMNL--------------VHP 333

Query: 302 PPHDGRRIRKTTSLNLKNRVENRASSPSRINGGNYSISSFF-STENLSTPPPSSLAGAGL 361
            P+D  R + TTS                        S+F   +  L  PP ++L GAG+
Sbjct: 334 SPNDEERDKTTTS------------------------SAFLEESSRLLKPPETTLGGAGV 393

Query: 362 SIHYAKIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPPSLYDPV 421
           ++HYA +++++EK    P L+G D RD+L++MLPA++R++LRSRL+        +  D  
Sbjct: 394 ALHYANLIVVMEKMIKQPQLVGLDARDDLYSMLPASVRSSLRSRLKGVGF----TATDGG 451

Query: 422 VAAEWKSAVAKILQWLAPMAHDTETWDSEQSFEKPLAGAGAGESRSHVLLLQTLHYADRE 481
           +A EWK+A+ +IL+WL P+A +   W SE+SFE+    A A  S++ V+L+QTL +AD+ 
Sbjct: 454 LATEWKAALGRILRWLLPLAQNMIRWQSERSFEQQHM-ATATNSQNRVMLVQTLVFADKV 451

Query: 482 KTEGAIVQLLVALSNI 487
           KTE AI +LLV L+ I
Sbjct: 514 KTEAAITELLVGLNYI 451

BLAST of MC05g1323 vs. TAIR 10
Match: AT1G30755.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 82.8 bits (203), Expect = 8.3e-16
Identity = 55/153 (35.95%), Postives = 83/153 (54.25%), Query Frame = 0

Query: 345 LAGAGLSIHYAKIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPP 404
           L  AGLS+HYA ++  I+  AS P  + ++ RD L+N LPAT++TALR RL++  +    
Sbjct: 355 LGEAGLSLHYANLIQQIDNIASRPSSLPSNVRDTLYNALPATVKTALRPRLQTLDQEEEL 414

Query: 405 SLYDPVVAAEWKSAVAKILQWLAPMAHDT-----------ETWDSEQSFEKPLAGAGAGE 464
           S+       E K+ + K LQWL P A +T           E  +S   F K   G G GE
Sbjct: 415 SV------PEIKAEMEKSLQWLVPFAENTTKAHQGFGWVGEWANSRIEFGK---GKGKGE 474

Query: 465 SRSHVLLLQTLHYADREKTEGAIVQLLVALSNI 487
           +  +   LQTLH+AD+   +  +++L+V L  +
Sbjct: 475 NNGNPTRLQTLHHADKPIVDSYVLELVVWLHRL 498

BLAST of MC05g1323 vs. TAIR 10
Match: AT1G34320.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 76.6 bits (187), Expect = 6.0e-14
Identity = 51/148 (34.46%), Postives = 82/148 (55.41%), Query Frame = 0

Query: 345 LAGAGLSIHYAKIVILIEKSASAPHLIGADDRDELFNMLPATIRTALRSRLRSAAKVRPP 404
           L  AGL++HYA I+  I+   S    + A  RD L+  LP +I++ALRSR++S       
Sbjct: 380 LGSAGLALHYANIITQIDTLVSRSSTMPASTRDALYQGLPPSIKSALRSRIQSF------ 439

Query: 405 SLYDPVVAAEWKSAVAKILQWLAPMAHDTETWDSEQSF----EKPLAGAGAGE--SRSHV 464
            + + +   + K+ + K LQWL P+A  T T  +   F    E   +G+ A +  +   +
Sbjct: 440 QVKEELTVPQIKAEMEKTLQWLVPVA--TNTTKAHHGFGWVGEWASSGSEANQRPAGQTI 499

Query: 465 LLLQTLHYADREKTEGAIVQLLVALSNI 487
           L + TLH+AD+EKTE  I+ L+V L ++
Sbjct: 500 LRIDTLHHADKEKTEAYILDLVVWLHHL 519