MC05g1250 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g1250
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	MC05: 16725389 .. 16726132 (-)
RNA-Seq Expression	MC05g1250
Synteny	MC05g1250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGCTAGAGGGAAAGGTAGCATTGATAACTGGTGGAGCCAGTGGCATTGGCGAATGCACGGCAAGAGTTTTTGCCCACCATGGTGCCAAAGTAGTGATTGCAGACATCCAAGACGACCTTGGCCACTCAGTTTGCAAGGTACTTGGCTCCTCCAACTCCCTCTATGTCCACTGTGACGTCACAGACGAATCCCACATAAAAAACGCCATCCACAAGGCCATCGAAGCCTTTGGAAAGTTGGACATCATGTTCAACAACGCAGGCATAGCTGACGCCAGCAAACCGCGAATCATCGACAATGAGAAAGAAGATTTCGAACGAGTGCTCAGCATAAACGTCACCGGAGTTTTCCTCGGCATCAAACACGCAGCTCAAGCAATGATCCCGACGAGAAGCGGAAGCATAATATCAACTGCAAGTGTGGCCTCATATGTTGGAGGTGCAGCCTCACATGCCTATACTTGCTCAAAGCACGCTGTGGTTGGGTTAACCAAAAATGCTGCCGTTGAGTTAGGCCAATTTGGGATTAGGGTTAATTGTTTATCTCCATATGCATTGGCCACACCTTTGGCTACAAAGTTTGTAGGGTTGGATAATGAGGAGTTTGAGAATTCAATGAACTCTAAGGCAAATTTAAAGGGTGTGACGCTTAAAGCTGAAGATGTGGCCAATGCAGCTTTGTTTTTGGCAAGCGATGAAGCGAAGTACGTGAGCGGACACAATCTATTGATCGATGGAGGTTTT

mRNA sequence

AGGCTAGAGGGAAAGGTAGCATTGATAACTGGTGGAGCCAGTGGCATTGGCGAATGCACGGCAAGAGTTTTTGCCCACCATGGTGCCAAAGTAGTGATTGCAGACATCCAAGACGACCTTGGCCACTCAGTTTGCAAGGTACTTGGCTCCTCCAACTCCCTCTATGTCCACTGTGACGTCACAGACGAATCCCACATAAAAAACGCCATCCACAAGGCCATCGAAGCCTTTGGAAAGTTGGACATCATGTTCAACAACGCAGGCATAGCTGACGCCAGCAAACCGCGAATCATCGACAATGAGAAAGAAGATTTCGAACGAGTGCTCAGCATAAACGTCACCGGAGTTTTCCTCGGCATCAAACACGCAGCTCAAGCAATGATCCCGACGAGAAGCGGAAGCATAATATCAACTGCAAGTGTGGCCTCATATGTTGGAGGTGCAGCCTCACATGCCTATACTTGCTCAAAGCACGCTGTGGTTGGGTTAACCAAAAATGCTGCCGTTGAGTTAGGCCAATTTGGGATTAGGGTTAATTGTTTATCTCCATATGCATTGGCCACACCTTTGGCTACAAAGTTTGTAGGGTTGGATAATGAGGAGTTTGAGAATTCAATGAACTCTAAGGCAAATTTAAAGGGTGTGACGCTTAAAGCTGAAGATGTGGCCAATGCAGCTTTGTTTTTGGCAAGCGATGAAGCGAAGTACGTGAGCGGACACAATCTATTGATCGATGGAGGTTTT

Coding sequence (CDS)

AGGCTAGAGGGAAAGGTAGCATTGATAACTGGTGGAGCCAGTGGCATTGGCGAATGCACGGCAAGAGTTTTTGCCCACCATGGTGCCAAAGTAGTGATTGCAGACATCCAAGACGACCTTGGCCACTCAGTTTGCAAGGTACTTGGCTCCTCCAACTCCCTCTATGTCCACTGTGACGTCACAGACGAATCCCACATAAAAAACGCCATCCACAAGGCCATCGAAGCCTTTGGAAAGTTGGACATCATGTTCAACAACGCAGGCATAGCTGACGCCAGCAAACCGCGAATCATCGACAATGAGAAAGAAGATTTCGAACGAGTGCTCAGCATAAACGTCACCGGAGTTTTCCTCGGCATCAAACACGCAGCTCAAGCAATGATCCCGACGAGAAGCGGAAGCATAATATCAACTGCAAGTGTGGCCTCATATGTTGGAGGTGCAGCCTCACATGCCTATACTTGCTCAAAGCACGCTGTGGTTGGGTTAACCAAAAATGCTGCCGTTGAGTTAGGCCAATTTGGGATTAGGGTTAATTGTTTATCTCCATATGCATTGGCCACACCTTTGGCTACAAAGTTTGTAGGGTTGGATAATGAGGAGTTTGAGAATTCAATGAACTCTAAGGCAAATTTAAAGGGTGTGACGCTTAAAGCTGAAGATGTGGCCAATGCAGCTTTGTTTTTGGCAAGCGATGAAGCGAAGTACGTGAGCGGACACAATCTATTGATCGATGGAGGTTTT

Protein sequence

RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGHNLLIDGGF

Homology

[Close]

BLAST of MC05g1250 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 2.4e-96
Identity = 175/249 (70.28%), Postives = 207/249 (83.13%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           RLEGKVALITGGASGIGE TA++F+ HGAKV IAD+QD+LGHSV + +G+SNS Y+HCDV
Sbjct: 14  RLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCDV 73

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           T+E  +KNA+   +  +GKLDIMF+NAGI+D ++PRIIDNEK DFERV S+NVTGVFL +
Sbjct: 74  TNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFLCM 133

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHAA+ MIP RSG+IISTAS++S +GG +SHAY  SKHAV+GLT+N AVELGQFGIRVNC
Sbjct: 134 KHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRVNC 193

Query: 181 LSPYALATPLATKFVGLDN-EEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSG 240
           LSP+ L T L  KF G+ N EEFEN +N   NLKG     EDVANAAL+LASDEAKYVSG
Sbjct: 194 LSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALYLASDEAKYVSG 253

Query: 241 HNLLIDGGF 249
           HNL IDGGF
Sbjct: 254 HNLFIDGGF 262

[Close]

BLAST of MC05g1250 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 2.6e-79
Identity = 153/248 (61.69%), Postives = 189/248 (76.21%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           +L GKVA+ITGGASGIG CTAR+F  HGA+VV+ADIQD+LG S+   LG   S YVHCDV
Sbjct: 14  KLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCDV 73

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           T+E  +  A+  A+  FGKLD+MFNNAG++     R+ +  KEDFERVL++N+ G FLG 
Sbjct: 74  TNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPFLGT 133

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHAA+ M P R GSIISTAS++S V GAASHAYT SKHA+VG T+NAA ELG+ GIRVNC
Sbjct: 134 KHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIRVNC 193

Query: 181 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGV-TLKAEDVANAALFLASDEAKYVSG 240
           +SP  +ATPLA   +G+D+E  E  M + ANLKG   LKA+D+A AALFLASD+ +YVSG
Sbjct: 194 VSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGRYVSG 253

Query: 241 HNLLIDGG 248
            NL +DGG
Sbjct: 254 QNLRVDGG 261

[Close]

BLAST of MC05g1250 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 7.7e-71
Identity = 135/250 (54.00%), Postives = 176/250 (70.40%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSL-YVHCD 60
           RL+ KVA+ITGGA GIGE TA++F  +GAKVVIADI DD G  VC  +GS + + +VHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLG 120
           VT +  ++N +   I   GKLDIMF N G+   +   I++   EDF+RV+ INV G FL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 IKHAAQAMIPTRSGSIISTASVASYVGG-AASHAYTCSKHAVVGLTKNAAVELGQFGIRV 180
            KHAA+ MIP + GSI+ TAS++S+  G   SH YT +KHAV+GLT +   ELGQ GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHGIRV 192

Query: 181 NCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVS 240
           NC+SPY +A+PL T   G+D+   E   +  ANLKG+ L+AEDVA+A  +LA DE+KYVS
Sbjct: 193 NCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLKGILLRAEDVADAVAYLAGDESKYVS 252

Query: 241 GHNLLIDGGF 249
           G NL+IDGG+
Sbjct: 253 GLNLVIDGGY 262

[Close]

BLAST of MC05g1250 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.2e-70
Identity = 134/250 (53.60%), Postives = 176/250 (70.40%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSL-YVHCD 60
           RL+ KVA+ITGGA GIGE TA++F  +GAKVVIADI DD G  VC  +GS + + +VHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLG 120
           VT +  ++N +   I   GKLDIMF N G+   +   I++   EDF+RV+ INV G FL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 IKHAAQAMIPTRSGSIISTASVASYVGG-AASHAYTCSKHAVVGLTKNAAVELGQFGIRV 180
            KHAA+ MIP + GSI+ TAS++S+  G   SH YT +KHAV+GLT +   ELG++GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIRV 192

Query: 181 NCLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVS 240
           NC+SPY +A+PL T   G+D+   E   +  ANLKG  L+AEDVA+A  +LA DE+KYVS
Sbjct: 193 NCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLKGTLLRAEDVADAVAYLAGDESKYVS 252

Query: 241 GHNLLIDGGF 249
           G NL+IDGG+
Sbjct: 253 GLNLVIDGGY 262

[Close]

BLAST of MC05g1250 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.3e-67
Identity = 137/255 (53.73%), Postives = 174/255 (68.24%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSS-NSLYVHCD 60
           RLEGKVAL+TGGASGIGE  AR+F  HGAK+ I D+QD+LG  V + LG   ++ Y HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 61  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLG 120
           VT E  ++ A+    E +G +DIM NNAGI       I D +  +F++V  INV GVFLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 121 IKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 180
           +KHAA+ MIP   GSI+S ASV+S + GA  H YT +KHAVVGLTK+ A ELG+ GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 181 CLSPYALATPLATKFVGLDNEE------FENSMNSKANLKGVTLKAEDVANAALFLASDE 240
           C+SPYA+ T L+  ++     +      F   + S ANLKGV L   DVA A L+LA++E
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLATEE 241

Query: 241 AKYVSGHNLLIDGGF 249
           +KYVSG NL+IDGGF
Sbjct: 242 SKYVSGLNLVIDGGF 256

[Close]

BLAST of MC05g1250 vs. NCBI nr
Match: XP_022144617.1 (secoisolariciresinol dehydrogenase-like [Momordica charantia])

HSP 1 Score: 484 bits (1246), Expect = 6.67e-172
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV
Sbjct: 8   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 67

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI
Sbjct: 68  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 127

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC
Sbjct: 128 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 187

Query: 181 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH 240
           LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH
Sbjct: 188 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH 247

Query: 241 NLLIDGGF 248
           NLLIDGGF
Sbjct: 248 NLLIDGGF 255

[Close]

BLAST of MC05g1250 vs. NCBI nr
Match: XP_023891279.1 (secoisolariciresinol dehydrogenase-like [Quercus suber])

HSP 1 Score: 422 bits (1085), Expect = 2.72e-147
Identity = 213/248 (85.89%), Postives = 231/248 (93.15%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           RLEGKVALITGGASGIGE TA+VF HHGAKVVIADIQDDLGHSV + LGSSN+LYVHCDV
Sbjct: 13  RLEGKVALITGGASGIGEFTAKVFVHHGAKVVIADIQDDLGHSVIEALGSSNALYVHCDV 72

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           TDE+ IKNA+ KA+  +GKLDIMFNNAGIAD +K RIIDNEK DFERVLS+NVTGVFLGI
Sbjct: 73  TDEAQIKNAVDKAVATYGKLDIMFNNAGIADENKARIIDNEKADFERVLSVNVTGVFLGI 132

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHA+QAMIP RSG IIST+S++SYVGGAAS+AYTCSKHAV+GLTKNAAVELGQFGIRVNC
Sbjct: 133 KHASQAMIPARSGCIISTSSISSYVGGAASYAYTCSKHAVLGLTKNAAVELGQFGIRVNC 192

Query: 181 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH 240
           LSPYALATPLATKFVG+D+E  EN MNS ANLKGVTLKAEDVANAALFLASDE+KYVSGH
Sbjct: 193 LSPYALATPLATKFVGVDDETLENMMNSLANLKGVTLKAEDVANAALFLASDESKYVSGH 252

Query: 241 NLLIDGGF 248
           NLLIDGGF
Sbjct: 253 NLLIDGGF 260

[Close]

BLAST of MC05g1250 vs. NCBI nr
Match: XP_030949167.1 (secoisolariciresinol dehydrogenase-like [Quercus lobata])

HSP 1 Score: 421 bits (1081), Expect = 1.11e-146
Identity = 212/248 (85.48%), Postives = 232/248 (93.55%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           RLEGKVALITGGASGIGE TA+VF HHGAKVVIADIQD+LGHSV + LGSSN+LYVHCDV
Sbjct: 13  RLEGKVALITGGASGIGEFTAKVFVHHGAKVVIADIQDELGHSVIEALGSSNALYVHCDV 72

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           TDE+ IKNA+ KA+  +GKLDIMFNNAGIAD +K RIIDNEK DFERVLS+NVTGVFLGI
Sbjct: 73  TDEAQIKNAVDKAVATYGKLDIMFNNAGIADENKVRIIDNEKADFERVLSVNVTGVFLGI 132

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHA+QAMIP RSGSIIST+S++SYVGGAAS++YTCSKHAVVGLTKNAAVELGQFGIRVNC
Sbjct: 133 KHASQAMIPARSGSIISTSSISSYVGGAASYSYTCSKHAVVGLTKNAAVELGQFGIRVNC 192

Query: 181 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH 240
           LSPYALATPLATKFVG+D+E  EN MNS ANLKGVTLKAEDVANAALFLASDE++YVSGH
Sbjct: 193 LSPYALATPLATKFVGVDDETLENVMNSLANLKGVTLKAEDVANAALFLASDESRYVSGH 252

Query: 241 NLLIDGGF 248
           NLLIDGGF
Sbjct: 253 NLLIDGGF 260

[Close]

BLAST of MC05g1250 vs. NCBI nr
Match: KAG2683042.1 (hypothetical protein I3760_10G013800 [Carya illinoinensis])

HSP 1 Score: 421 bits (1082), Expect = 1.70e-146
Identity = 211/248 (85.08%), Postives = 229/248 (92.34%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           RLEGKVALITGGASGIGECTA+VFA HGA+VV+ADIQD+LGH + + LG SNSLYVHCDV
Sbjct: 35  RLEGKVALITGGASGIGECTAKVFARHGARVVVADIQDELGHELIQALGPSNSLYVHCDV 94

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           TDES IKNA+ KA+  FGKLDIMFNNAGIAD +K RIIDNEK DFERVLS+NVTGVFLGI
Sbjct: 95  TDESQIKNAVEKAVATFGKLDIMFNNAGIADENKARIIDNEKSDFERVLSVNVTGVFLGI 154

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHAAQAMIP RSG IISTASV+SYVGGAASHAY CSKHAV+GLTKNAAVELGQFGIRVNC
Sbjct: 155 KHAAQAMIPVRSGCIISTASVSSYVGGAASHAYACSKHAVLGLTKNAAVELGQFGIRVNC 214

Query: 181 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH 240
           LSPYAL TPLA KFVGL++E FEN+MNS ANLKGVTLKAEDVANAAL+LASDE++YVSGH
Sbjct: 215 LSPYALVTPLARKFVGLEDEAFENTMNSLANLKGVTLKAEDVANAALYLASDESRYVSGH 274

Query: 241 NLLIDGGF 248
           NLLIDGGF
Sbjct: 275 NLLIDGGF 282

[Close]

BLAST of MC05g1250 vs. NCBI nr
Match: XP_042946954.1 (secoisolariciresinol dehydrogenase-like [Carya illinoinensis] >KAG6638128.1 hypothetical protein CIPAW_10G014100 [Carya illinoinensis] >KAG7958304.1 hypothetical protein I3843_10G014000 [Carya illinoinensis])

HSP 1 Score: 421 bits (1082), Expect = 1.70e-146
Identity = 211/248 (85.08%), Postives = 229/248 (92.34%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           RLEGKVALITGGASGIGECTA+VFA HGA+VV+ADIQD+LGH + + LG SNSLYVHCDV
Sbjct: 35  RLEGKVALITGGASGIGECTAKVFARHGARVVVADIQDELGHELIQALGPSNSLYVHCDV 94

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           TDES IKNA+ KA+  FGKLDIMFNNAGIAD +K RIIDNEK DFERVLS+NVTGVFLGI
Sbjct: 95  TDESQIKNAVEKAVATFGKLDIMFNNAGIADENKARIIDNEKSDFERVLSVNVTGVFLGI 154

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHAAQAMIP RSG IISTASV+SYVGGAASHAY CSKHAV+GLTKNAAVELGQFGIRVNC
Sbjct: 155 KHAAQAMIPVRSGCIISTASVSSYVGGAASHAYACSKHAVLGLTKNAAVELGQFGIRVNC 214

Query: 181 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH 240
           LSPYAL TPLA KFVGL++E FEN+MNS ANLKGVTLKAEDVANAAL+LASDE++YVSGH
Sbjct: 215 LSPYALVTPLARKFVGLEDEAFENTMNSLANLKGVTLKAEDVANAALYLASDESRYVSGH 274

Query: 241 NLLIDGGF 248
           NLLIDGGF
Sbjct: 275 NLLIDGGF 282

[Close]

BLAST of MC05g1250 vs. ExPASy TrEMBL
Match: A0A6J1CU62 (secoisolariciresinol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111014261 PE=4 SV=1)

HSP 1 Score: 484 bits (1246), Expect = 3.23e-172
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV
Sbjct: 8   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 67

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI
Sbjct: 68  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 127

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC
Sbjct: 128 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 187

Query: 181 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH 240
           LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH
Sbjct: 188 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH 247

Query: 241 NLLIDGGF 248
           NLLIDGGF
Sbjct: 248 NLLIDGGF 255

[Close]

BLAST of MC05g1250 vs. ExPASy TrEMBL
Match: A0A7N2N7S8 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 421 bits (1081), Expect = 5.35e-147
Identity = 212/248 (85.48%), Postives = 232/248 (93.55%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           RLEGKVALITGGASGIGE TA+VF HHGAKVVIADIQD+LGHSV + LGSSN+LYVHCDV
Sbjct: 13  RLEGKVALITGGASGIGEFTAKVFVHHGAKVVIADIQDELGHSVIEALGSSNALYVHCDV 72

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           TDE+ IKNA+ KA+  +GKLDIMFNNAGIAD +K RIIDNEK DFERVLS+NVTGVFLGI
Sbjct: 73  TDEAQIKNAVDKAVATYGKLDIMFNNAGIADENKVRIIDNEKADFERVLSVNVTGVFLGI 132

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHA+QAMIP RSGSIIST+S++SYVGGAAS++YTCSKHAVVGLTKNAAVELGQFGIRVNC
Sbjct: 133 KHASQAMIPARSGSIISTSSISSYVGGAASYSYTCSKHAVVGLTKNAAVELGQFGIRVNC 192

Query: 181 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH 240
           LSPYALATPLATKFVG+D+E  EN MNS ANLKGVTLKAEDVANAALFLASDE++YVSGH
Sbjct: 193 LSPYALATPLATKFVGVDDETLENVMNSLANLKGVTLKAEDVANAALFLASDESRYVSGH 252

Query: 241 NLLIDGGF 248
           NLLIDGGF
Sbjct: 253 NLLIDGGF 260

[Close]

BLAST of MC05g1250 vs. ExPASy TrEMBL
Match: A0A5A7TKJ5 (Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004260 PE=4 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 6.23e-146
Identity = 208/249 (83.53%), Postives = 230/249 (92.37%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVC-KVLGSSNSLYVHCD 60
           RLEGKVALITGGASGIGECTA++F HHGAKVVIADIQDDLGH++C  VLGS+NSLYVHCD
Sbjct: 15  RLEGKVALITGGASGIGECTAKIFVHHGAKVVIADIQDDLGHALCANVLGSTNSLYVHCD 74

Query: 61  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLG 120
           VTDES ++ A+  A+E FGKLD+M NNAGI D SKPRIIDN++ DF+RVL+INVTGVFLG
Sbjct: 75  VTDESQVQAAVAAAVETFGKLDVMMNNAGIVDPSKPRIIDNDRSDFDRVLNINVTGVFLG 134

Query: 121 IKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 180
           IKHAAQAMIP ++GSI+STASVASY+GG+ASHAYTCSKHAVVGLTKNAAVELGQFGIRVN
Sbjct: 135 IKHAAQAMIPAKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 194

Query: 181 CLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSG 240
           CLSPYAL TPLATKFVGL+  EFE  M SKANLKGVTLKAEDVANAALFLASDE++YVSG
Sbjct: 195 CLSPYALVTPLATKFVGLNGAEFEEVMGSKANLKGVTLKAEDVANAALFLASDESRYVSG 254

Query: 241 HNLLIDGGF 248
           HNLLIDGGF
Sbjct: 255 HNLLIDGGF 263

[Close]

BLAST of MC05g1250 vs. ExPASy TrEMBL
Match: A0A7N2M6N8 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 6.23e-146
Identity = 211/248 (85.08%), Postives = 231/248 (93.15%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           RLEGKVALITGGASGIGE TA+VF HHGAKVVIADIQD+LGHSV +  GSSN+LYVHCDV
Sbjct: 13  RLEGKVALITGGASGIGEFTAKVFVHHGAKVVIADIQDELGHSVIEAHGSSNALYVHCDV 72

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           TDE+ IKNA+ KA+  +GKLDIMFNNAGIAD +K RIIDNEK DFERVLS+NVTGVFLGI
Sbjct: 73  TDEAQIKNAVDKAVATYGKLDIMFNNAGIADENKVRIIDNEKADFERVLSVNVTGVFLGI 132

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHA+QAMIP RSGSIIST+S++SYVGGAAS++YTCSKHAVVGLTKNAAVELGQFGIRVNC
Sbjct: 133 KHASQAMIPARSGSIISTSSISSYVGGAASYSYTCSKHAVVGLTKNAAVELGQFGIRVNC 192

Query: 181 LSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSGH 240
           LSPYALATPLATKFVG+D+E  EN MNS ANLKGVTLKAEDVANAALFLASDE++YVSGH
Sbjct: 193 LSPYALATPLATKFVGVDDETLENVMNSLANLKGVTLKAEDVANAALFLASDESRYVSGH 252

Query: 241 NLLIDGGF 248
           NLLIDGGF
Sbjct: 253 NLLIDGGF 260

[Close]

BLAST of MC05g1250 vs. ExPASy TrEMBL
Match: A0A1S3B7H5 (secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103486649 PE=4 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 6.23e-146
Identity = 208/249 (83.53%), Postives = 230/249 (92.37%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVC-KVLGSSNSLYVHCD 60
           RLEGKVALITGGASGIGECTA++F HHGAKVVIADIQDDLGH++C  VLGS+NSLYVHCD
Sbjct: 15  RLEGKVALITGGASGIGECTAKIFVHHGAKVVIADIQDDLGHALCANVLGSTNSLYVHCD 74

Query: 61  VTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLG 120
           VTDES ++ A+  A+E FGKLD+M NNAGI D SKPRIIDN++ DF+RVL+INVTGVFLG
Sbjct: 75  VTDESQVQAAVAAAVETFGKLDVMMNNAGIVDPSKPRIIDNDRSDFDRVLNINVTGVFLG 134

Query: 121 IKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 180
           IKHAAQAMIP ++GSI+STASVASY+GG+ASHAYTCSKHAVVGLTKNAAVELGQFGIRVN
Sbjct: 135 IKHAAQAMIPAKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 194

Query: 181 CLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSG 240
           CLSPYAL TPLATKFVGL+  EFE  M SKANLKGVTLKAEDVANAALFLASDE++YVSG
Sbjct: 195 CLSPYALVTPLATKFVGLNGAEFEEVMGSKANLKGVTLKAEDVANAALFLASDESRYVSG 254

Query: 241 HNLLIDGGF 248
           HNLLIDGGF
Sbjct: 255 HNLLIDGGF 263

[Close]

BLAST of MC05g1250 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 4.8e-68
Identity = 139/260 (53.46%), Postives = 170/260 (65.38%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVL----GSSNSLYV 60
           RL GKVALITGGA+GIGE   R+F  HGAKV I D+QDDLG  VCK L        + ++
Sbjct: 17  RLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFFI 76

Query: 61  HCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGV 120
           H DV  E  I NA+  A++ FG LDI+ NNAG+  A  P I +    +FE    +NV G 
Sbjct: 77  HGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDVNVKGA 136

Query: 121 FLGIKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGI 180
           FL +KHAA+ MIP + GSI+S  SV   VGG   H+Y  SKHAV+GLT++ A ELGQ GI
Sbjct: 137 FLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQHGI 196

Query: 181 RVNCLSPYALATPLATKFVGLDNEE--------FENSMNSKANLKGVTLKAEDVANAALF 240
           RVNC+SPYA+AT LA     L  EE        F N   + ANLKGV L  +DVANA LF
Sbjct: 197 RVNCVSPYAVATKLA--LAHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDVANAVLF 256

Query: 241 LASDEAKYVSGHNLLIDGGF 249
           LASD+++Y+SG NL+IDGGF
Sbjct: 257 LASDDSRYISGDNLMIDGGF 274

[Close]

BLAST of MC05g1250 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 6.9e-67
Identity = 135/263 (51.33%), Postives = 180/263 (68.44%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNS----LYV 60
           RLEGKVA+ITGGA GIG+ T  +FA HGA VVIAD+ +  G S+ K L S  +     ++
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 61  HCDVTDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPR--IIDNEKEDFERVLSINVT 120
            CDV+ E+ ++N ++  +  +G+LDI+FNNAG+    K    I+D + ++F+ V+ +NV 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 121 GVFLGIKHAAQAMIPTR-SGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQ 180
           GV LG+KH A+AMI     G IISTASVA  +GG   HAYT SKHA+VGLTKNAA ELG+
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 181 FGIRVNCLSPYALATPLATK---------FVGLDNEEFENSMNSKANLKGVTLKAEDVAN 240
           +GIRVNC+SP+ +AT +                D EE E  + S ANLKG TL+A D+A 
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIAE 270

Query: 241 AALFLASDEAKYVSGHNLLIDGG 248
           AAL+LASDE+KYV+GHNL++DGG
Sbjct: 271 AALYLASDESKYVNGHNLVVDGG 293

[Close]

BLAST of MC05g1250 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 6.1e-63
Identity = 119/249 (47.79%), Postives = 172/249 (69.08%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           RL+GK+ +ITGGASGIG  + R+F  HGA+VVI D+QD+LG +V   +G   + Y HCDV
Sbjct: 5   RLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCDV 64

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           T+E+ ++NA+   +E +GKLD++F+NAG+ +     I+D    + +R ++IN+ G    I
Sbjct: 65  TNETEVENAVKFTVEKYGKLDVLFSNAGVIEPF-VSILDLNLNELDRTIAINLRGTAAFI 124

Query: 121 KHAAQAMIPTR-SGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 180
           KHAA+AM+     GSI+ T SVA+ + G A H YT SKH ++GL K+A+  LG++GIRVN
Sbjct: 125 KHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIRVN 184

Query: 181 CLSPYALATPLATKFVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAKYVSG 240
            ++P+ +ATPL      ++    E + ++ ANLKG+ LKA  VA AALFLASDE+ YVSG
Sbjct: 185 GVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKGIVLKARHVAEAALFLASDESAYVSG 244

Query: 241 HNLLIDGGF 249
            NL +DGG+
Sbjct: 245 QNLAVDGGY 252

[Close]

BLAST of MC05g1250 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 236.1 bits (601), Expect = 3.0e-62
Identity = 126/253 (49.80%), Postives = 165/253 (65.22%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           +LEGKVALITGGASG+G+ TA  F  HGA+VVIAD+  + G    K LG S + +V CDV
Sbjct: 40  KLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELG-SEAEFVRCDV 99

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDN-EKEDFERVLSINVTGVFLG 120
           T E+ I  A+   +E +GKLD+M+NNAGI     P  I   +  +FERV+ INV GV  G
Sbjct: 100 TVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINVFGVVSG 159

Query: 121 IKHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 180
           IKHAA+ MIP RSG I+ T+SVA   GG A H+YT SK    G+ K+AA EL + G+R+N
Sbjct: 160 IKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVRIN 219

Query: 181 CLSPYALATPLATK-----FVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEA 240
           C+SP  +ATPL        F  +  E+   ++     LKG   +  DVA AAL+LAS++ 
Sbjct: 220 CISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGMGELKGAECEEADVAKAALYLASNDG 279

Query: 241 KYVSGHNLLIDGG 248
           KYV+GHNL++DGG
Sbjct: 280 KYVTGHNLVVDGG 291

[Close]

BLAST of MC05g1250 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 235.3 bits (599), Expect = 5.1e-62
Identity = 127/253 (50.20%), Postives = 165/253 (65.22%), Query Frame = 0


Query: 1   RLEGKVALITGGASGIGECTARVFAHHGAKVVIADIQDDLGHSVCKVLGSSNSLYVHCDV 60
           +LEGKVALITGGASGIG+ TA  F  HGAKV+IADIQ  +G    + LG S   Y  CDV
Sbjct: 77  KLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELGPS-CAYFPCDV 136

Query: 61  TDESHIKNAIHKAIEAFGKLDIMFNNAGIADASKPRIIDNEKEDFERVLSINVTGVFLGI 120
           T ES I NA+  A+    KLDIM+NNAGI   + P I+D +   F++V++ NV GV  GI
Sbjct: 137 TKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKVINTNVRGVMAGI 196

Query: 121 KHAAQAMIPTRSGSIISTASVASYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNC 180
           KHAA+ MIP  SGSII   SV   +GG A H Y+ SK AV+G+ ++ A EL +  IRVNC
Sbjct: 197 KHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHRIRVNC 256

Query: 181 LSPYALATPLATK-----FVGLDNEEFENSMNSKANLKGVTLKAEDVANAALFLASDEAK 240
           +SP+A+ T          + G+D+      + S   L G   +  DVANAA++LASD++K
Sbjct: 257 ISPFAITTSFVMDEMRQIYPGVDDSRLIQIVQSTGVLNGEVCEPTDVANAAVYLASDDSK 316

Query: 241 YVSGHNLLIDGGF 249
           YV+GHNL++DGGF
Sbjct: 317 YVNGHNLVVDGGF 328

The following BLAST results are available for this feature:

BLAST of MC05g1250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	2.4e-96	70.28	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q7FAE1	2.6e-79	61.69	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
A3F5F0	7.7e-71	54.00	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
Q94KL8	2.2e-70	53.60	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
F1SWA0	2.3e-67	53.73	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]

BLAST of MC05g1250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144617.1	6.67e-172	100.00	secoisolariciresinol dehydrogenase-like [Momordica charantia]	[more]
XP_023891279.1	2.72e-147	85.89	secoisolariciresinol dehydrogenase-like [Quercus suber]	[more]
XP_030949167.1	1.11e-146	85.48	secoisolariciresinol dehydrogenase-like [Quercus lobata]	[more]
KAG2683042.1	1.70e-146	85.08	hypothetical protein I3760_10G013800 [Carya illinoinensis]	[more]
XP_042946954.1	1.70e-146	85.08	secoisolariciresinol dehydrogenase-like [Carya illinoinensis] >KAG6638128.1 hypo...	[more]

BLAST of MC05g1250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CU62	3.23e-172	100.00	secoisolariciresinol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A7N2N7S8	5.35e-147	85.48	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]
A0A5A7TKJ5	6.23e-146	83.53	Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A7N2M6N8	6.23e-146	85.08	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]
A0A1S3B7H5	6.23e-146	83.53	secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103486649 ...	[more]

BLAST of MC05g1250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52340.1	4.8e-68	53.46	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G51680.1	6.9e-67	51.33	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	6.1e-63	47.79	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	3.0e-62	49.80	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G03140.1	5.1e-62	50.20	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 6..23 score: 49.89 coord: 127..143 score: 33.53 coord: 153..172 score: 42.87 coord: 212..232 score: 34.88 coord: 78..89 score: 58.12 coord: 174..191 score: 37.48
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 133..141 score: 46.12 coord: 153..172 score: 37.39 coord: 78..89 score: 51.1
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 11..248 e-value: 1.5E-69 score: 234.2
None	No IPR available	GENE3D	3.40.50.720		coord: 1..248 e-value: 5.0E-82 score: 277.0
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 2..248 e-value: 1.6E-23 score: 80.2
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 8..201 e-value: 4.1E-20 score: 69.2
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 1..248
None	No IPR available	PANTHER	PTHR43180:SF50	SECOISOLARICIRESINOL DEHYDROGENASE-LIKE	coord: 1..248
None	No IPR available	CDD	cd05326	secoisolariciresinol-DH_like_SDR_c	coord: 1..248 e-value: 1.64523E-121 score: 344.054
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 140..168
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 3..248

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g1250.1	MC05g1250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity