MC05g1177 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g1177 vs. ExPASy Swiss-Prot
Match: Q9M1S8 (Probable glutamate carboxypeptidase AMP1 OS=Arabidopsis thaliana OX=3702 GN=AMP1 PE=1 SV=3)

HSP 1 Score: 776.5 bits (2004), Expect = 2.5e-223
Identity = 397/705 (56.31%), Postives = 516/705 (73.19%), Query Frame = 0

Query: 3   QPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFS---TSSLSATSSPRNSLRFQQL 62
           +P +  ++ I   +P PL +FLF+I++ +  FYTLH     T  L  + +  N+LR ++L
Sbjct: 8   RPTVTGISIIPFRQPPPLCSFLFVIVLFVATFYTLHHPDAVTPPLLFSRNAYNALRLRRL 67

Query: 63  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 122
           FLSSASN T++SYLR LT HPHLAGT+PS +T  YV +HF+ LGLETH  +++ALLSYP 
Sbjct: 68  FLSSASNATISSYLRELTRHPHLAGTKPSLDTLHYVFNHFQSLGLETHVAEYEALLSYPT 127

Query: 123 YASLSARLSNGSVVNIPSSE--NIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAA 182
           + S++A  SN + +    ++      VV+PYHAYSPSG+A G  VFVN+G + DY AL +
Sbjct: 128 HISVTASFSNTTTLEFDLNDVPGDSPVVRPYHAYSPSGSAQGNVVFVNHGEERDYHALES 187

Query: 183 IGVGVAGCIVVARKGD-FPRGVVVAKAEANGAKGVLLYAEGDRFR-QGFERGTVMRGIGD 242
           IGV V GC+V+ARKG+   RG +V  AEA GA GVL+YAE D     G ERGTVMRGIGD
Sbjct: 188 IGVSVKGCVVLARKGENLGRGAIVKIAEAKGALGVLIYAENDGGGFGGIERGTVMRGIGD 247

Query: 243 PLSPGWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTA 302
           P+SPGW  + G E+L+L+D  V +RFPKIPS+PLS   AEIIL+SL  A  P EWR+ + 
Sbjct: 248 PVSPGWPGVVGGEKLSLDDELVTRRFPKIPSLPLSLRNAEIILASLGGARAPLEWRN-SG 307

Query: 303 KLRSSSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAV 362
           ++     VGP G   +N T+QGE K+  I NV+  I+G EE DR+V++GNHRDAW++GAV
Sbjct: 308 RVGPGQRVGP-GRMVINMTFQGEMKMKKINNVVVTIRGSEEADRYVILGNHRDAWTYGAV 367

Query: 363 DPNSGTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKA 422
           DPNSGT+ALLDI+RRFALL + GW PRRTILLCSWDAEEFGM+GSTEW+E+N++NLG  A
Sbjct: 368 DPNSGTSALLDISRRFALLLKSGWRPRRTILLCSWDAEEFGMIGSTEWIEENVLNLGASA 427

Query: 423 VAYLNVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERL 482
           VAYLNVDCAVQG GFF+GATPQLD LL+ V   VQDPD  G TV +T+ + N    I+RL
Sbjct: 428 VAYLNVDCAVQGSGFFAGATPQLDGLLVDVLKLVQDPDAVGLTVEETFKSQNNI--IQRL 487

Query: 483 GALNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGL 542
             ++SDF+ F+ HAG+PS+D+YYG D+PVYHTAFD+YDWM +  DP FHRHV +  IWGL
Sbjct: 488 SRVDSDFSGFLHHAGIPSIDMYYGADYPVYHTAFDSYDWMIHNADPLFHRHVAMAGIWGL 547

Query: 543 LALRLADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEA 602
           L + LAD+ ++PF Y+SYA+QLQA++D L+  L+  VS+ PLS AIQE    A+E  +EA
Sbjct: 548 LGILLADEPLIPFDYISYADQLQAHRDKLSKLLEGKVSVNPLSMAIQEFSLVAKEAADEA 607

Query: 603 KRLREQETSSD--VALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESAL 662
           K+L+ +  S +   A  ++R LNDRLML ERGFLD +G++G+ WFKHLVYGP ++ ES L
Sbjct: 608 KKLKGKSYSKNDVAAAAKRRELNDRLMLVERGFLDAEGIKGKEWFKHLVYGPAAEPESKL 667

Query: 663 VYFPGIADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKG 699
            +FPGIADA++    MN  E    I+HEIWRV RAI+RA+ ALKG
Sbjct: 668 GFFPGIADAIA----MNASE--GIIEHEIWRVARAIQRASKALKG 702

BLAST of MC05g1177 vs. ExPASy Swiss-Prot
Match: A0A1D6L709 (Probable glutamate carboxypeptidase VP8 OS=Zea mays OX=4577 GN=VP8 PE=2 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 6.7e-197
Identity = 347/652 (53.22%), Postives = 462/652 (70.86%), Query Frame = 0

Query: 58  QLFLSSA--SNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALL 117
           +LFLS +  +N ++A+ LR+LT  PHLAGT  S+  A +V +  R  GL+T + +++ LL
Sbjct: 56  RLFLSQSPGANASIAADLRALTAGPHLAGTPASAGAAAHVLARLRAAGLQTLTREYEPLL 115

Query: 118 SYPKYASLSARLSNGSV-----VNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDE 177
           SYP +ASL+    +GS+     +  P+ E  + VV PYHAY+PSG A   AVFVN GR+E
Sbjct: 116 SYPGHASLALLRPDGSLLARLSLEEPADEG-RRVVPPYHAYAPSGGAVAEAVFVNLGREE 175

Query: 178 DYRALAAIGVGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTV- 237
           DY  L  +GVGV G + VAR+G   RG VVA+A   GA  VL+    D    G ERG V 
Sbjct: 176 DYVVLERLGVGVRGRVAVARRGGGYRGGVVARAADKGAVAVLIAGNAD---GGVERGVVL 235

Query: 238 MRGIGDPLSPGWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPE 297
           + G GDPL+PGWAA  GAERL  +D  V +RFP IPSMP+SA+ A  I+ SL   ++P E
Sbjct: 236 LGGPGDPLTPGWAATSGAERLKFDDKAVKQRFPSIPSMPVSAKTAAAIIRSLGGPAIPAE 295

Query: 298 WRDTTAKLRSSSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDA 357
           W+D       +  +GP GPT +NFTYQ +RK   IR++  +IKG EEPDR+V++GNHRDA
Sbjct: 296 WKDGLGV--DTGGLGP-GPTLVNFTYQEDRKFYKIRDIFGIIKGQEEPDRYVILGNHRDA 355

Query: 358 WSFGAVDPNSGTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIV 417
           W++GAVDPNSGTAALLDIARR  ++ + GW PRR+I+LCSWD EEFGM+GSTEWVE N+ 
Sbjct: 356 WTYGAVDPNSGTAALLDIARRLGIMLQSGWKPRRSIILCSWDGEEFGMIGSTEWVEDNLE 415

Query: 418 NLGTKAVAYLNVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGF 477
           +L +KAVAYLNVDCAVQG GFF+G+TPQLD LL+ +T QV+DPDV G  V+DTW   +G 
Sbjct: 416 DLHSKAVAYLNVDCAVQGVGFFAGSTPQLDKLLVDITRQVRDPDVTGKMVHDTWNEMSGG 475

Query: 478 GNIERLGALNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTV 537
             IERL   +SDFA F+ HAG+PSVD+YYG +FP YHTA DTY+WM  +GDP F RH+ +
Sbjct: 476 IKIERLARTDSDFAPFLHHAGIPSVDLYYGEEFPGYHTALDTYNWMEKHGDPFFLRHLAI 535

Query: 538 GNIWGLLALRLADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQ 597
             IWGLLALRLA+D +LPF Y +Y +QLQ +  TL+       ++  ++  + +L  AA 
Sbjct: 536 TEIWGLLALRLANDPVLPFDYQAYTSQLQEHIKTLSALTSNGHAVNLMNGCVNDLSGAAM 595

Query: 598 EIENEAKRLREQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDY 657
           E+  E K+L++ +   + A +++R LNDRL+LAER FL  +GL+GR WFKHL+Y PP DY
Sbjct: 596 EVLKEMKKLQQMDLYDEHARMRRRLLNDRLLLAERSFLQPEGLQGRGWFKHLLYSPPEDY 655

Query: 658 ESALVYFPGIADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 702
           ES L +FPGIADA+S S  ++ +E++ A+QHE+W+V RAI+RAA+ L+G+ S
Sbjct: 656 ESKLSFFPGIADAISRSANLSDKEQEVAMQHEVWKVCRAIQRAASVLRGEFS 700

BLAST of MC05g1177 vs. ExPASy Swiss-Prot
Match: Q852M4 (Probable glutamate carboxypeptidase PLA3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA3 PE=1 SV=2)

HSP 1 Score: 682.6 bits (1760), Expect = 4.8e-195
Identity = 343/648 (52.93%), Postives = 460/648 (70.99%), Query Frame = 0

Query: 59  LFLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYP 118
           L LS  +N T+A+ LR+LT  PHLAGT  ++  A  V S FR  GL T + ++  LLSYP
Sbjct: 92  LALSEGANATIAADLRALTAGPHLAGTGAAAGAAARVLSGFRAAGLRTLTREYTPLLSYP 151

Query: 119 KYASLSARLSNGSVVNIPSSENIQGV----VKPYHAYSPSGAAYGRAVFVNYGRDEDYRA 178
            +ASL+   ++ +++   S +    V    V+PYHAY+PSG A   AVFVN GR+EDY  
Sbjct: 152 GHASLALLRADRTLLAHLSLDEPADVGRRLVRPYHAYAPSGGAVAEAVFVNLGREEDYLT 211

Query: 179 LAAIGVGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTV-MRGI 238
           L  +GV V G + VA +G   RG VV +A    A  VL+    D    G ERGTV + G 
Sbjct: 212 LERLGVSVRGRVAVAIRGGGYRGGVVRRAAERSAAAVLIAGHAD---GGVERGTVILGGP 271

Query: 239 GDPLSPGWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDT 298
           GDPL+PGWAA  GAERL+ +  +V +RFP IPSMP+S + A  I+ +L   ++P +W+  
Sbjct: 272 GDPLTPGWAATAGAERLDFDHEDVKRRFPAIPSMPVSGKTASAIIRTLGGPALPADWQTG 331

Query: 299 TAKLRSSSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFG 358
                    VGP GPT +NFTYQ +RK+  I+++ A+IKG EEPDR+V++GNHRDAW++G
Sbjct: 332 VGLPVDVGGVGP-GPTLVNFTYQEDRKMGMIQDIFAIIKGYEEPDRYVILGNHRDAWTYG 391

Query: 359 AVDPNSGTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGT 418
           AVDPNSGT+ALLDIARR  ++ + GW+PRRTI+LCSWDAEEFGM+GSTEWVE+N+ +L +
Sbjct: 392 AVDPNSGTSALLDIARRLGIMLQSGWTPRRTIILCSWDAEEFGMIGSTEWVEENLEDLQS 451

Query: 419 KAVAYLNVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIE 478
           KAVAYLNVDCAVQG G F+G+TPQLD+LL+ VT QV+DPDV+G TV+DTW    G  NIE
Sbjct: 452 KAVAYLNVDCAVQGIGLFAGSTPQLDNLLVDVTRQVKDPDVEGKTVHDTWNKMTGGINIE 511

Query: 479 RLGALNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIW 538
           RL   +SDFA F+ HAG+P +D+YYG++FP YHTA D+Y WM  +GDP F RHV +  IW
Sbjct: 512 RLARTDSDFAPFLHHAGIPCMDLYYGKEFPGYHTALDSYHWMEKHGDPLFLRHVAIVEIW 571

Query: 539 GLLALRLADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIEN 598
           GLLALRLADD +LPF Y +YA+QLQ + +  +  ++ S  +  L+ +I++L  A  E   
Sbjct: 572 GLLALRLADDPVLPFDYQTYASQLQEHANAFSSMMENSKWVHLLNRSIEDLSDAGLEFLK 631

Query: 599 EAKRLREQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESAL 658
           EAK+L++Q  S   +L+++R LNDRL+LAER FL  DGL+GR WFKHL+Y PP DYES L
Sbjct: 632 EAKKLQDQNISDGYSLMRRRLLNDRLLLAERSFLQADGLQGRGWFKHLMYSPPEDYESKL 691

Query: 659 VYFPGIADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 702
            +FPG+ADA+S S+  + +E+QAA++HE+ ++ RAI+RAA  L+G+ S
Sbjct: 692 SFFPGVADAISRSSNRSAKEQQAAVRHEVRKISRAIQRAADVLRGEFS 735

BLAST of MC05g1177 vs. ExPASy Swiss-Prot
Match: Q7Y228 (Probable glutamate carboxypeptidase LAMP1 OS=Arabidopsis thaliana OX=3702 GN=LAMP1 PE=2 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 3.4e-156
Identity = 303/698 (43.41%), Postives = 429/698 (61.46%), Query Frame = 0

Query: 32  LGFYTLHFSTSSLSATSSPRNSLRFQQLFLSS--ASNYTVASYLRSLTLHPHLAGTEPSS 91
           L F     S S  S  SSP  S  + +LF+S+  + N +VA  L +LT  PH+AGT  ++
Sbjct: 7   LAFVIAALSYSFFSLFSSPPKS-HYHELFISTSFSDNASVALNLHTLTRRPHVAGTVANA 66

Query: 92  ETARYVESHFRDLGLETHSIQFDALLSYPKYASLSARLSNGS--VVNIPSSENI------ 151
           E A YV S F    L++H + +   L+YP + SL    ++ +  +  +   E +      
Sbjct: 67  EAAEYVRSVFTSSALKSHVVAYQVSLTYPVHRSLVLTPTDSAKPITFLLEQEKLGDNPYA 126

Query: 152 QGVVKPYHAYSPSGAAYGRAVFVNYGRDEDY-RALAAIGVGVAGCIVVARKGDFPRGVVV 211
             V+  +H Y+ SG   G  V+ NYGR ED+ R    +GV V+G +V+AR G   RG +V
Sbjct: 127 NEVMPTFHGYAKSGNVSGPVVYANYGRVEDFVRLKKDMGVNVSGAVVIARYGQIYRGDIV 186

Query: 212 AKAEANGAKGVLLYAE-----GDRF--------RQGFERGTVMRGIGDPLSPGWAAIDGA 271
             A   GA GV++Y +     GD +          G + GTV  G+GDP +PGWA++DG 
Sbjct: 187 KNAYEAGAVGVVIYTDKRDYGGDEWFPASKWMPPSGVQVGTVYNGLGDPTTPGWASVDGC 246

Query: 272 ERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSS----LDTASVPPEWRDTTAKLRSSSSV 331
           ERL+    E+    P IPS+P+SA  AE+IL +    +    V P              V
Sbjct: 247 ERLSDEAVELSGDVPLIPSLPVSAADAEVILKTVVGDVSDGDVYP--------------V 306

Query: 332 GPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAA 391
           GP GP  LN +Y GE  ++ I NV+ VI+G EEPDR+V++GNHRDAW+FGAVDPNSGTA 
Sbjct: 307 GP-GPGVLNLSYIGETVIAKIENVIGVIEGEEEPDRYVILGNHRDAWTFGAVDPNSGTAV 366

Query: 392 LLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYLNVDC 451
           L++IA+R   L++ GW PRRTI+LC+WDAEE+G++GSTEWVE+N   L ++AVAYLNVDC
Sbjct: 367 LMEIAQRLDKLQKRGWKPRRTIILCNWDAEEYGLIGSTEWVEENREMLSSRAVAYLNVDC 426

Query: 452 AVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALNSDFA 511
           AV GPGF + ATPQLD+L+     +V+DPD    T+Y++W  ++    I RLG   SD+A
Sbjct: 427 AVSGPGFHASATPQLDELIKVAAQEVRDPDNATQTIYESWIGSSDSVVIRRLGGGGSDYA 486

Query: 512 AFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALRLADD 571
           +FVQH GVP VD+ +GR +PVYH+ +D + WM  +GDP F RHV + ++ GL+ALRLAD+
Sbjct: 487 SFVQHVGVPGVDMSFGRGYPVYHSMYDDFTWMEKFGDPMFQRHVAMASVLGLVALRLADE 546

Query: 572 LILPFSYVSYANQL-QAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLREQE 631
            I+PF+Y SYA +L ++ +D  N+ L  ++ +  L  +I++L +AA+ I  E + ++   
Sbjct: 547 EIIPFNYTSYALELKKSAEDLENEKLGHNIDVSTLIKSIEDLSTAAKHISLEKEAIK--- 606

Query: 632 TSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIADA 691
                  L+ R LNDRLM+AER   D DGL  RPW+KHL+YGP    +     FPG+ DA
Sbjct: 607 -----GALKVRELNDRLMMAERALTDRDGLSERPWYKHLIYGPSKYDDYGSKSFPGVDDA 666

Query: 692 VSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDL 701
           +  + K+N +     +QH+IWRV RAIR A+  LKG+L
Sbjct: 667 IDNAKKLNTKASWENVQHQIWRVSRAIRHASLVLKGEL 680

BLAST of MC05g1177 vs. ExPASy Swiss-Prot
Match: O35409 (Glutamate carboxypeptidase 2 OS=Mus musculus OX=10090 GN=Folh1 PE=1 SV=2)

HSP 1 Score: 358.2 bits (918), Expect = 2.1e-97
Identity = 249/728 (34.20%), Postives = 380/728 (52.20%), Query Frame = 0

Query: 31  LLGFYTLHFSTSSLSATSSPRNSLRFQQLFLSSASNYTVASYLRSLTLHPHLAGTEPSSE 90
           L+GF    F   S  AT +  +S   ++ FL       +  +L + T  PHLAGT+ + E
Sbjct: 35  LIGFLFGWFIKPSNEATGNVSHS-GMKKEFLHELKAENIKKFLYNFTRTPHLAGTQNNFE 94

Query: 91  TARYVESHFRDLGLETHSI-QFDALLSY-----PKYASLSARLSNG------SVVNIPSS 150
            A+ +   +++ GL+   +  +D LLSY     P Y S+     N       S    P  
Sbjct: 95  LAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYISIINEDGNEIFKTSLSEQPPPGY 154

Query: 151 ENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRAL-AAIGVGVAGCIVVARKGDFPRG 210
           ENI  VV PY A+SP G   G  V+VNY R ED+  L   + +  +G IV+AR G   RG
Sbjct: 155 ENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLEREMKISCSGKIVIARYGKVFRG 214

Query: 211 VVVAKAEANGAKGVLLYAE-GDRF--------------RQGFERGTV--MRGIGDPLSPG 270
            +V  A+  GAKG++LY++  D F                G +RG V  + G GDPL+PG
Sbjct: 215 NMVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPG 274

Query: 271 WAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVP-PEWRDTTAKLRS 330
           + A + A R  L ++  L   P IP  P+  + A+ +L  +   + P   W+     L+ 
Sbjct: 275 YPANEHAYRHELTNAVGL---PSIPVHPIGYDDAQKLLEHMGGPAPPDSSWK---GGLKV 334

Query: 331 SSSVGPGGPTFLNFTYQGER-------KVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSF 390
             +VGPG     NF+ Q  +       KV+ I NV+  +KG  EPDR+V++G HRDAW F
Sbjct: 335 PYNVGPGFAG--NFSTQKVKMHIHSYTKVTRIYNVIGTLKGALEPDRYVILGGHRDAWVF 394

Query: 391 GAVDPNSGTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLG 450
           G +DP SG A + +I R F  L++ G  PRRTIL  SWDAEEFG++GSTEW E++   L 
Sbjct: 395 GGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILFASWDAEEFGLLGSTEWAEEHSRLLQ 454

Query: 451 TKAVAYLNVDCAVQGPGFFS-GATPQLDDLLLKVTAQVQDPD--VKGATVYDTWTATN-- 510
            + VAY+N D +++G        TP +  L+  +T ++Q PD   +G ++YD+W   +  
Sbjct: 455 ERGVAYINADSSIEGNYTLRVDCTPLMYSLVYNLTKELQSPDEGFEGKSLYDSWKEKSPS 514

Query: 511 ----GFGNIERLGALNSDFAAFVQHAGVPSVDVYYGRD--------FPVYHTAFDTYDWM 570
               G   I +LG+ N DF  F Q  G+ S    Y ++        +P+YH+ ++TY+ +
Sbjct: 515 PEFIGMPRISKLGSGN-DFEVFFQRLGIASGRARYTKNWKTNKVSSYPLYHSVYETYELV 574

Query: 571 ANYGDPSFHRHVTVGNIWGLLALRLADDLILPFSYVSYANQLQAYKDTL-NDFLDESVSL 630
             + DP+F  H+TV  + G +   LA+ ++LPF   SYA  L+ Y DT+ N  +     +
Sbjct: 575 VKFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAVALKKYADTIYNISMKHPQEM 634

Query: 631 LPLSTAIQELQSAAQEIENEA----KRLREQETSSDVALLQKRALNDRLMLAERGFLDVD 690
                +   L SA     + A    +RL+E + S+ + L   R +ND+LM  ER F+D  
Sbjct: 635 KAYMISFDSLFSAVNNFTDVASKFNQRLQELDKSNPILL---RIMNDQLMYLERAFIDPL 694

Query: 691 GLRGRPWFKHLVYGPPSDYESALVYFPGIADAVSE-STKMNQRERQAAIQHEIWRVVRAI 698
           GL GRP+++H++Y P S  + A   FPGI DA+ + S+K+N  +    ++ +I      +
Sbjct: 695 GLPGRPFYRHIIYAPSSHNKYAGESFPGIYDALFDISSKVNASKAWNEVKRQISIATFTV 749

BLAST of MC05g1177 vs. NCBI nr
Match: XP_038905595.1 (probable glutamate carboxypeptidase AMP1 [Benincasa hispida])

HSP 1 Score: 1238 bits (3204), Expect = 0.0
Identity = 625/702 (89.03%), Postives = 657/702 (93.59%), Query Frame = 0

Query: 1   MAQPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFST-SSLSATSSPRNSLRFQQL 60
           MAQPPLKQL TICTSKPAPL TFLF++IIC+LGF+T HFS+ SS SATS+P NS+RFQQL
Sbjct: 1   MAQPPLKQLATICTSKPAPLPTFLFVVIICVLGFFTFHFSSLSSFSATSTPSNSVRFQQL 60

Query: 61  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 120
            LSSASNYTVASYLRSLTLHPHLAGTEPS ET +YVESHFR+LGLETHSIQ+DALLSYPK
Sbjct: 61  LLSSASNYTVASYLRSLTLHPHLAGTEPSLETVQYVESHFRELGLETHSIQYDALLSYPK 120

Query: 121 YASLSARLSNGSVVNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAAIG 180
            ASLSARL NGSVVNIP SE ++GVV+PYHAYSPSG AYG AVFVNYGRDEDYRALA IG
Sbjct: 121 SASLSARLPNGSVVNIPLSEYVEGVVQPYHAYSPSGTAYGTAVFVNYGRDEDYRALAKIG 180

Query: 181 VGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTVMRGIGDPLSP 240
           V V GCI VARKG+FPRGVVVAKAEANGAKGVLLY EGD FRQGFERGTVMRGIGDPLSP
Sbjct: 181 VAVDGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEGDGFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTAKLRS 300
           GWAAIDGAERL+LNDSEVLKRFPKIPSMPLSAEAAEIILSSLD+ASVPPEWRD   KL  
Sbjct: 241 GWAAIDGAERLSLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDSASVPPEWRDMKGKL-G 300

Query: 301 SSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360
           S++VGPGGPTF+NFTYQGERKV+TIRNV+AVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS
Sbjct: 301 SAAVGPGGPTFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360

Query: 361 GTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYL 420
           GTAALLDIARRFALLRRLGW+PRRTILLCSWDAEEFGM+GSTEWVEQNIVNLGTKAVAYL
Sbjct: 361 GTAALLDIARRFALLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYL 420

Query: 421 NVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALN 480
           NVDCAVQGPGFFSGATPQLDDLL +VTAQVQDPDVKGATVYDTWTA NG GNIERLGALN
Sbjct: 421 NVDCAVQGPGFFSGATPQLDDLLHEVTAQVQDPDVKGATVYDTWTAKNGIGNIERLGALN 480

Query: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALR 540
           SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDP F RHVTVG+IWGLLALR
Sbjct: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFQRHVTVGSIWGLLALR 540

Query: 541 LADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLR 600
           L+DDLILPFSYVSYANQLQAY+DTLND LD SVSL  LST+IQEL+SAAQEIENEAKRLR
Sbjct: 541 LSDDLILPFSYVSYANQLQAYQDTLNDVLDGSVSLHTLSTSIQELKSAAQEIENEAKRLR 600

Query: 601 EQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660
           EQE  SDVAL QKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFP I
Sbjct: 601 EQEIFSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPSI 660

Query: 661 ADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 701
           ADAVSES KMN+RE +  IQHEIWRV RAIRRAA ALKG+LS
Sbjct: 661 ADAVSESKKMNKREMEEVIQHEIWRVARAIRRAALALKGELS 701

BLAST of MC05g1177 vs. NCBI nr
Match: XP_008442988.1 (PREDICTED: probable glutamate carboxypeptidase 2 isoform X1 [Cucumis melo] >KAA0043770.1 putative glutamate carboxypeptidase 2 isoform X1 [Cucumis melo var. makuwa] >TYK25363.1 putative glutamate carboxypeptidase 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1224 bits (3167), Expect = 0.0
Identity = 614/702 (87.46%), Postives = 649/702 (92.45%), Query Frame = 0

Query: 1   MAQPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFSTSS-LSATSSPRNSLRFQQL 60
           M QPPLKQL TICTS+PAPL TF F+IIIC+LGFY  HFS+SS  S TSSPRNS+RFQQL
Sbjct: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60

Query: 61  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 120
            LSS SNYTVASYLRSLTLHPHLAGTEPSSET RYVESHFRDLGLETHSIQ+DALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 YASLSARLSNGSVVNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAAIG 180
             SLS   SNGSVVNIP SEN++GVV+PYHAYSPSG  YG AVFVNYGRDEDYR LA +G
Sbjct: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTVMRGIGDPLSP 240
           V V GCI VARKG+FPRGVVVAKAEANGAKGVLLY E DRFRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTAKLRS 300
           GWAA+DGAERLNLNDSEVLKRFPKIPSMPLS+E+AEIIL+SLDTASVPPEWRD  A LRS
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300

Query: 301 SSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360
           ++ VGPGGP F+NFTYQGERKV+TIRNV+AVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS
Sbjct: 301 AA-VGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360

Query: 361 GTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYL 420
           GTAALLDIARRFALLR+LGW+PRRTILLCSWDAEEFGM+GSTEWVEQNIVNLGTKAVAYL
Sbjct: 361 GTAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYL 420

Query: 421 NVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALN 480
           NVDCAVQGPGFF+GATPQLDDLL  VTAQVQDPDVKGATV+DTWTA NG GNIERLGA+N
Sbjct: 421 NVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVN 480

Query: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALR 540
           SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDP FHRHVTVG+IWGLLALR
Sbjct: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALR 540

Query: 541 LADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLR 600
           L+DDLILPFSYVSYANQLQAYKDTLN  LD SVSL  LST+I+EL+SAAQEIENEAKRLR
Sbjct: 541 LSDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLR 600

Query: 601 EQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660
           EQETSSDVAL QKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI
Sbjct: 601 EQETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660

Query: 661 ADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 701
           ADAVSES +MN+   +  IQHEIWRV RAI RAA ALKG+LS
Sbjct: 661 ADAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 701

BLAST of MC05g1177 vs. NCBI nr
Match: XP_023539721.1 (probable glutamate carboxypeptidase AMP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1216 bits (3147), Expect = 0.0
Identity = 608/702 (86.61%), Postives = 650/702 (92.59%), Query Frame = 0

Query: 1   MAQPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFSTSS-LSATSSPRNSLRFQQL 60
           MA PPLKQLTT+C+SKP+P+TT LF IIIC+LGFYT H S+SS  S TSS RNS+R++Q 
Sbjct: 1   MAPPPLKQLTTLCSSKPSPVTTLLFFIIICVLGFYTFHSSSSSSFSVTSSLRNSVRYRQF 60

Query: 61  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 120
            LSSASNYTVASYLRSLTLHPHLAGTEPSSET RYVESHFRDLG ETHSI++DALLSYPK
Sbjct: 61  LLSSASNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGFETHSIRYDALLSYPK 120

Query: 121 YASLSARLSNGSVVNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAAIG 180
           YASL+ARL NGSVV IP SEN++GVVKPYHAYSPSG AYG AVFVNYGRDEDYRALAA+G
Sbjct: 121 YASLTARLPNGSVVQIPLSENVEGVVKPYHAYSPSGTAYGVAVFVNYGRDEDYRALAAMG 180

Query: 181 VGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTVMRGIGDPLSP 240
           V VAGCI VARKG+FPRGVVVAKAEANG KGVLLYA+ D +RQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVAGCIAVARKGEFPRGVVVAKAEANGVKGVLLYADSDGYRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTAKLRS 300
           GWAAIDGAERLNLNDSEVL++FPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTT KL  
Sbjct: 241 GWAAIDGAERLNLNDSEVLRKFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTIKL-G 300

Query: 301 SSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360
           +++VGPGGPT+LNFTYQGERK++TI NVLAVIKGLEEPDRFV+MGNHRDAWSFGAVDPNS
Sbjct: 301 TAAVGPGGPTYLNFTYQGERKIATIHNVLAVIKGLEEPDRFVIMGNHRDAWSFGAVDPNS 360

Query: 361 GTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYL 420
           GTAALLDIARRFALLR+LGWSPRRT+LLCSWDAEEFGM+GSTEWVEQNIVNLGTKAVAYL
Sbjct: 361 GTAALLDIARRFALLRKLGWSPRRTLLLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYL 420

Query: 421 NVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALN 480
           NVDCAVQGPGFFSGATPQLDDLL +VTAQV+DPDVKGATVYDTWTATNG  NIERLGALN
Sbjct: 421 NVDCAVQGPGFFSGATPQLDDLLHEVTAQVEDPDVKGATVYDTWTATNGIANIERLGALN 480

Query: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALR 540
           SDFAAFVQHAGVPS+DVYYGRDFPVYHTAFDTYDWMA+YGDP FHRH  VG+IWGLLALR
Sbjct: 481 SDFAAFVQHAGVPSIDVYYGRDFPVYHTAFDTYDWMASYGDPLFHRHAAVGSIWGLLALR 540

Query: 541 LADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLR 600
           LADDLILPFSYVSYANQLQAY+D LND LD SVSL PLST+I E +SAA+EIENEAKRLR
Sbjct: 541 LADDLILPFSYVSYANQLQAYRDILNDILDGSVSLQPLSTSIHEFKSAAEEIENEAKRLR 600

Query: 601 EQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660
           EQET + VAL Q RALNDRLMLAERGFLDVDGLRGR WFKHLVYGP SDYESAL YFPGI
Sbjct: 601 EQETFTAVALFQTRALNDRLMLAERGFLDVDGLRGRTWFKHLVYGPSSDYESALAYFPGI 660

Query: 661 ADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 701
           ADA+SESTKMNQ E Q  IQHEIWRVVRAI RAA ALKG+LS
Sbjct: 661 ADAISESTKMNQSEGQGVIQHEIWRVVRAITRAAAALKGELS 701

BLAST of MC05g1177 vs. NCBI nr
Match: KAG6596649.1 (putative glutamate carboxypeptidase AMP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1215 bits (3143), Expect = 0.0
Identity = 609/702 (86.75%), Postives = 650/702 (92.59%), Query Frame = 0

Query: 1   MAQPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFSTSS-LSATSSPRNSLRFQQL 60
           MA PPLKQLTT+C+SKP+P+TT LF IIIC+LGFYT H S+SS  SATSS RNS+R++Q 
Sbjct: 1   MAPPPLKQLTTLCSSKPSPVTTLLFFIIICVLGFYTFHSSSSSSFSATSSLRNSVRYRQF 60

Query: 61  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 120
            LSSASNYTVASYLRSLTLHPHLAGTEPSSET RYVESHFRDLG ETHSI++DALLSYPK
Sbjct: 61  LLSSASNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGFETHSIRYDALLSYPK 120

Query: 121 YASLSARLSNGSVVNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAAIG 180
           YASL+ARL NGSVV IP SEN++GVVKPYHAYSPSG AYG AVFVNYGRDEDYRALAA+G
Sbjct: 121 YASLTARLPNGSVVQIPLSENVEGVVKPYHAYSPSGTAYGVAVFVNYGRDEDYRALAAMG 180

Query: 181 VGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTVMRGIGDPLSP 240
           V VAGCI V RKG+FPRGVVVAKAEANG KGVLLYA+ D +RQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVAGCIAVVRKGEFPRGVVVAKAEANGVKGVLLYADSDGYRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTAKLRS 300
           GWAAIDGAERLNLNDSEVL++FPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTT KL  
Sbjct: 241 GWAAIDGAERLNLNDSEVLRKFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTIKL-G 300

Query: 301 SSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360
           +++VGPGGPT+LNFTYQGERK++TI NVLAVIKGLEEPDRFV+MGNHRDAWSFGAVDPNS
Sbjct: 301 TAAVGPGGPTYLNFTYQGERKIATIHNVLAVIKGLEEPDRFVIMGNHRDAWSFGAVDPNS 360

Query: 361 GTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYL 420
           GTAALLDIARRFALLR+LGWSPRRT+LLCSWDAEEFGM+GSTEWVEQNIVNLGTKAVAYL
Sbjct: 361 GTAALLDIARRFALLRKLGWSPRRTLLLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYL 420

Query: 421 NVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALN 480
           NVDCAVQGPGFFSGATPQLDDLL +VTAQV+DPDVKGATVYDTWTATNG GNIERLGALN
Sbjct: 421 NVDCAVQGPGFFSGATPQLDDLLHEVTAQVEDPDVKGATVYDTWTATNGIGNIERLGALN 480

Query: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALR 540
           SDFAAFVQHAGVPS+DVYYGRDFPVYHTAFDTYDWMA+YGDP FHRH  VG+IWGLLALR
Sbjct: 481 SDFAAFVQHAGVPSIDVYYGRDFPVYHTAFDTYDWMASYGDPLFHRHAAVGSIWGLLALR 540

Query: 541 LADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLR 600
           LADDLILPFSYVSYANQLQAY+D LND LD SVSL  LST+I E +SAA+EIENEAKRLR
Sbjct: 541 LADDLILPFSYVSYANQLQAYRDILNDILDGSVSLQSLSTSIHEFKSAAEEIENEAKRLR 600

Query: 601 EQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660
           EQET + VAL Q RALNDRLMLAERGFLDVDGLRGR WFKHLVYGP SDYESAL YFPGI
Sbjct: 601 EQETFTAVALFQTRALNDRLMLAERGFLDVDGLRGRTWFKHLVYGPSSDYESALSYFPGI 660

Query: 661 ADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 701
           ADAVSES KMNQ E Q  IQHEIWRVVRAI RAATALKG+LS
Sbjct: 661 ADAVSESMKMNQSEGQGVIQHEIWRVVRAITRAATALKGELS 701

BLAST of MC05g1177 vs. NCBI nr
Match: XP_004136724.3 (LOW QUALITY PROTEIN: probable glutamate carboxypeptidase AMP1 [Cucumis sativus])

HSP 1 Score: 1212 bits (3135), Expect = 0.0
Identity = 611/702 (87.04%), Postives = 648/702 (92.31%), Query Frame = 0

Query: 1   MAQPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFSTSS-LSATSSPRNSLRFQQL 60
           M Q PLKQL TICTS+PAPL TF F+IIIC+LGFYT HFSTSS  S TSSPRNS+RFQQL
Sbjct: 1   MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFSVTSSPRNSVRFQQL 60

Query: 61  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 120
            LSS SNYTVASYLRSLTLHPHLAGTEPSSET RYVESHFRDLGLETHSIQ+DALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 YASLSARLSNGSVVNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAAIG 180
             SLS  LSNG+VVNIP SEN++GVV+PYHAYSPSG AYG AVFVNYGRDEDYR LA +G
Sbjct: 121 STSLSVLLSNGTVVNIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTVMRGIGDPLSP 240
           V V GCI VARKG+FPRGVVVAKAEANGAKGVLLY EGD FRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTAKLRS 300
           GWAA+DGAERLNLNDSEVLKRFPKIPSMPLSAE+AEIILSSLDTASVPPEWR   A L  
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRKK-ANL-G 300

Query: 301 SSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360
           S++VGPGGP F+NFTYQGERKV+TIRNV+AVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS
Sbjct: 301 SAAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360

Query: 361 GTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYL 420
           GTAALLDIARRF+LLRRLGW+PRRTILLCSWDAEEFGM+GSTEWVEQNIVNLGTKAVAYL
Sbjct: 361 GTAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYL 420

Query: 421 NVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALN 480
           NVDCAVQGPGFF+GATPQLDDLL  VTAQVQDPDVKGATV+DTWTA NG GNIERLGA+N
Sbjct: 421 NVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVN 480

Query: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALR 540
           SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDP FHRHVTVG+IWGLLALR
Sbjct: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALR 540

Query: 541 LADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLR 600
           L+DDLILPFSY+SYANQLQAYKDTLN  LD SVSL  LS++I+EL+ AAQEIENEAKRLR
Sbjct: 541 LSDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLR 600

Query: 601 EQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660
           EQE SSDVAL QKRALNDRLMLAERGFLDVDGLRG PWFKHLVYGP S+YESALVYFPGI
Sbjct: 601 EQEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGI 660

Query: 661 ADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 701
           ADAVSES ++N+RE +  IQHEIWRV RAIRRAA ALKG+LS
Sbjct: 661 ADAVSESKEINKRELEELIQHEIWRVARAIRRAAAALKGELS 700

BLAST of MC05g1177 vs. ExPASy TrEMBL
Match: A0A5D3DNZ1 (Putative glutamate carboxypeptidase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005020 PE=3 SV=1)

HSP 1 Score: 1224 bits (3167), Expect = 0.0
Identity = 614/702 (87.46%), Postives = 649/702 (92.45%), Query Frame = 0

Query: 1   MAQPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFSTSS-LSATSSPRNSLRFQQL 60
           M QPPLKQL TICTS+PAPL TF F+IIIC+LGFY  HFS+SS  S TSSPRNS+RFQQL
Sbjct: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60

Query: 61  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 120
            LSS SNYTVASYLRSLTLHPHLAGTEPSSET RYVESHFRDLGLETHSIQ+DALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 YASLSARLSNGSVVNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAAIG 180
             SLS   SNGSVVNIP SEN++GVV+PYHAYSPSG  YG AVFVNYGRDEDYR LA +G
Sbjct: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTVMRGIGDPLSP 240
           V V GCI VARKG+FPRGVVVAKAEANGAKGVLLY E DRFRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTAKLRS 300
           GWAA+DGAERLNLNDSEVLKRFPKIPSMPLS+E+AEIIL+SLDTASVPPEWRD  A LRS
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300

Query: 301 SSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360
           ++ VGPGGP F+NFTYQGERKV+TIRNV+AVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS
Sbjct: 301 AA-VGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360

Query: 361 GTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYL 420
           GTAALLDIARRFALLR+LGW+PRRTILLCSWDAEEFGM+GSTEWVEQNIVNLGTKAVAYL
Sbjct: 361 GTAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYL 420

Query: 421 NVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALN 480
           NVDCAVQGPGFF+GATPQLDDLL  VTAQVQDPDVKGATV+DTWTA NG GNIERLGA+N
Sbjct: 421 NVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVN 480

Query: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALR 540
           SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDP FHRHVTVG+IWGLLALR
Sbjct: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALR 540

Query: 541 LADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLR 600
           L+DDLILPFSYVSYANQLQAYKDTLN  LD SVSL  LST+I+EL+SAAQEIENEAKRLR
Sbjct: 541 LSDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLR 600

Query: 601 EQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660
           EQETSSDVAL QKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI
Sbjct: 601 EQETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660

Query: 661 ADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 701
           ADAVSES +MN+   +  IQHEIWRV RAI RAA ALKG+LS
Sbjct: 661 ADAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 701

BLAST of MC05g1177 vs. ExPASy TrEMBL
Match: A0A1S3B7R6 (probable glutamate carboxypeptidase 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486721 PE=3 SV=1)

HSP 1 Score: 1224 bits (3167), Expect = 0.0
Identity = 614/702 (87.46%), Postives = 649/702 (92.45%), Query Frame = 0

Query: 1   MAQPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFSTSS-LSATSSPRNSLRFQQL 60
           M QPPLKQL TICTS+PAPL TF F+IIIC+LGFY  HFS+SS  S TSSPRNS+RFQQL
Sbjct: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60

Query: 61  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 120
            LSS SNYTVASYLRSLTLHPHLAGTEPSSET RYVESHFRDLGLETHSIQ+DALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 YASLSARLSNGSVVNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAAIG 180
             SLS   SNGSVVNIP SEN++GVV+PYHAYSPSG  YG AVFVNYGRDEDYR LA +G
Sbjct: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTVMRGIGDPLSP 240
           V V GCI VARKG+FPRGVVVAKAEANGAKGVLLY E DRFRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTAKLRS 300
           GWAA+DGAERLNLNDSEVLKRFPKIPSMPLS+E+AEIIL+SLDTASVPPEWRD  A LRS
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300

Query: 301 SSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360
           ++ VGPGGP F+NFTYQGERKV+TIRNV+AVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS
Sbjct: 301 AA-VGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360

Query: 361 GTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYL 420
           GTAALLDIARRFALLR+LGW+PRRTILLCSWDAEEFGM+GSTEWVEQNIVNLGTKAVAYL
Sbjct: 361 GTAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYL 420

Query: 421 NVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALN 480
           NVDCAVQGPGFF+GATPQLDDLL  VTAQVQDPDVKGATV+DTWTA NG GNIERLGA+N
Sbjct: 421 NVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVN 480

Query: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALR 540
           SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDP FHRHVTVG+IWGLLALR
Sbjct: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALR 540

Query: 541 LADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLR 600
           L+DDLILPFSYVSYANQLQAYKDTLN  LD SVSL  LST+I+EL+SAAQEIENEAKRLR
Sbjct: 541 LSDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLR 600

Query: 601 EQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660
           EQETSSDVAL QKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI
Sbjct: 601 EQETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660

Query: 661 ADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 701
           ADAVSES +MN+   +  IQHEIWRV RAI RAA ALKG+LS
Sbjct: 661 ADAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 701

BLAST of MC05g1177 vs. ExPASy TrEMBL
Match: A0A0A0LEA0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G790960 PE=3 SV=1)

HSP 1 Score: 1214 bits (3142), Expect = 0.0
Identity = 611/702 (87.04%), Postives = 647/702 (92.17%), Query Frame = 0

Query: 1   MAQPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFSTSS-LSATSSPRNSLRFQQL 60
           M Q PLKQL TICTS+PAPL TF F+IIIC+LGFYT HFSTSS  S TSSPRNS+RFQQL
Sbjct: 1   MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFSVTSSPRNSVRFQQL 60

Query: 61  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 120
            LSS SNYTVASYLRSLTLHPHLAGTEPSSET RYVESHFRDLGLETHSIQ+DALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 YASLSARLSNGSVVNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAAIG 180
             SLS  LSNG+VV IP SEN++GVV+PYHAYSPSG AYG AVFVNYGRDEDYR LA +G
Sbjct: 121 STSLSVLLSNGTVVYIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTVMRGIGDPLSP 240
           V V GCI VARKG+FPRGVVVAKAEANGAKGVLLY EGD FRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTAKLRS 300
           GWAA+DGAERLNLNDSEVLKRFPKIPSMPLSAE+AEIILSSLDTASVPPEWRD  A L  
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANL-G 300

Query: 301 SSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360
           S++VGPGGP F+NFTYQGERKV+TIRNV+AVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS
Sbjct: 301 SAAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360

Query: 361 GTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYL 420
           GTAALLDIARRF+LLRRLGW+PRRTILLCSWDAEEFGM+GSTEWVEQNIVNLGTKAVAYL
Sbjct: 361 GTAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYL 420

Query: 421 NVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALN 480
           NVDCAVQGPGFF+GATPQLDDLL  VTAQVQDPDVKGATV+DTWTA NG GNIERLGA+N
Sbjct: 421 NVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVN 480

Query: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALR 540
           SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDP FHRHVTVG+IWGLLALR
Sbjct: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALR 540

Query: 541 LADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLR 600
           L+DDLILPFSY+SYANQLQAYKDTLN  LD SVSL  LS++I+EL+ AAQEIENEAKRLR
Sbjct: 541 LSDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLR 600

Query: 601 EQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660
           EQE SSDVAL QKRALNDRLMLAERGFLDVDGLRG PWFKHLVYGP S+YESALVYFPGI
Sbjct: 601 EQEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGI 660

Query: 661 ADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 701
           ADAVSES  +N+RE +  IQHEIWRV RAIRRAA ALKG+LS
Sbjct: 661 ADAVSESKVINKRELEELIQHEIWRVARAIRRAAAALKGELS 701

BLAST of MC05g1177 vs. ExPASy TrEMBL
Match: A0A6J1FMI1 (probable glutamate carboxypeptidase AMP1 OS=Cucurbita moschata OX=3662 GN=LOC111446753 PE=3 SV=1)

HSP 1 Score: 1207 bits (3124), Expect = 0.0
Identity = 604/702 (86.04%), Postives = 649/702 (92.45%), Query Frame = 0

Query: 1   MAQPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFSTSS-LSATSSPRNSLRFQQL 60
           MA PPLKQLTT+C+SKP+P+TT LF IIIC+LGFYT H S+SS  SATSS RNS+R++Q 
Sbjct: 1   MAPPPLKQLTTLCSSKPSPVTTLLFFIIICVLGFYTFHSSSSSSFSATSSLRNSVRYRQF 60

Query: 61  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 120
            LSSASNYTVASYLRSLTLHPHLAGTEPSSET RYVESHFRDLG ETHSI++DALLSYPK
Sbjct: 61  LLSSASNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGFETHSIRYDALLSYPK 120

Query: 121 YASLSARLSNGSVVNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAAIG 180
           YASL+ARL NGSVV IP SEN++GVVKPYHAYSPSG AYG AVFVNYGRDEDYRALAA+G
Sbjct: 121 YASLTARLPNGSVVQIPLSENVEGVVKPYHAYSPSGTAYGVAVFVNYGRDEDYRALAAMG 180

Query: 181 VGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTVMRGIGDPLSP 240
           V VAGCI VARKG+FPRGVVVAKAEANG KGVLLYA+ D +RQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVAGCIAVARKGEFPRGVVVAKAEANGVKGVLLYADSDGYRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTAKLRS 300
           GWAAIDGAERLNLNDSEVL++FPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTT KL  
Sbjct: 241 GWAAIDGAERLNLNDSEVLRKFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTIKL-G 300

Query: 301 SSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360
           +++VGPGGPT+LNFTYQGERK++TI NVLAVIKGLEEPDRFV+MGNHRDAWSFGAVDPNS
Sbjct: 301 TAAVGPGGPTYLNFTYQGERKIATIHNVLAVIKGLEEPDRFVIMGNHRDAWSFGAVDPNS 360

Query: 361 GTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYL 420
           GTAALLDIARRFALLR+LGWSPRRT+LLCSWDAEEFGM+GSTEWVEQNIVNLGTKAVAYL
Sbjct: 361 GTAALLDIARRFALLRKLGWSPRRTLLLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYL 420

Query: 421 NVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALN 480
           NVDCAVQGPGFFSGATPQLDDLL +VTAQV+DPDVKGATVYDTWTATNG GNIERLGALN
Sbjct: 421 NVDCAVQGPGFFSGATPQLDDLLHEVTAQVEDPDVKGATVYDTWTATNGIGNIERLGALN 480

Query: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALR 540
           SDFAAFVQHAGVPS+DVYYGRDFPVYHTAFDTYDWMA+YGDP FHRH  VG+IWGLLALR
Sbjct: 481 SDFAAFVQHAGVPSIDVYYGRDFPVYHTAFDTYDWMASYGDPLFHRHAAVGSIWGLLALR 540

Query: 541 LADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLR 600
           LADDLILPFSY SYANQLQAY+D LND LD SV+L  LST+I E +SAA+EIENEAKRLR
Sbjct: 541 LADDLILPFSYASYANQLQAYRDKLNDILDGSVALQSLSTSIHEFKSAAKEIENEAKRLR 600

Query: 601 EQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660
           EQET + VAL Q RALNDRLMLAERGFLDV+GLRGR WFKHLVYGP SDYESAL YFPGI
Sbjct: 601 EQETFTAVALFQTRALNDRLMLAERGFLDVEGLRGRTWFKHLVYGPSSDYESALSYFPGI 660

Query: 661 ADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 701
           A+A+SES KMNQ E Q  IQHEIWRVVRAI RAA ALKG+LS
Sbjct: 661 ANAISESMKMNQSEGQGVIQHEIWRVVRAITRAAAALKGELS 701

BLAST of MC05g1177 vs. ExPASy TrEMBL
Match: A0A6J1KUI3 (probable glutamate carboxypeptidase AMP1 OS=Cucurbita maxima OX=3661 GN=LOC111498787 PE=3 SV=1)

HSP 1 Score: 1193 bits (3087), Expect = 0.0
Identity = 597/702 (85.04%), Postives = 641/702 (91.31%), Query Frame = 0

Query: 1   MAQPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFSTSS-LSATSSPRNSLRFQQL 60
           MA  PLKQLTT+C+SKP+P+TT LF IIIC+LGFYT H S+SS   ATSS RNS+R++Q 
Sbjct: 1   MAPMPLKQLTTLCSSKPSPVTTLLFFIIICVLGFYTFHSSSSSSFFATSSLRNSVRYRQF 60

Query: 61  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 120
            LSSASNYTVASYLRSLTLHPHLAGTEPSSET RYVESHFRDLG ETHSI++DALLSYPK
Sbjct: 61  LLSSASNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGFETHSIRYDALLSYPK 120

Query: 121 YASLSARLSNGSVVNIPSSENIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAAIG 180
           YASL+ARL NGSVV IP SEN++GVV PYHAYSPSG AYG AVFVNYGRDEDYR LAA+G
Sbjct: 121 YASLTARLPNGSVVQIPLSENVEGVVNPYHAYSPSGTAYGVAVFVNYGRDEDYRTLAAMG 180

Query: 181 VGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAEGDRFRQGFERGTVMRGIGDPLSP 240
           V VAGCI VARKG+FPR VVVAKAEANG KGVLLY + D +RQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVAGCIAVARKGEFPRSVVVAKAEANGVKGVLLYTDSDGYRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTAKLRS 300
           GWAAIDGAERLNLNDSEVL++FPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTT KL  
Sbjct: 241 GWAAIDGAERLNLNDSEVLRKFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTVKL-G 300

Query: 301 SSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNS 360
           +++VGPGGPT+LNFTYQ E+K++TI NVLAVIKGLEEPDRFV+MGNHRDAWSFGAVDPNS
Sbjct: 301 TAAVGPGGPTYLNFTYQAEKKIATIHNVLAVIKGLEEPDRFVIMGNHRDAWSFGAVDPNS 360

Query: 361 GTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYL 420
           GTAALLDIARRF+LLR+LGWSPRRT+LLCSWDAEEFGM+GSTEWVEQNIVNLGTKAVAYL
Sbjct: 361 GTAALLDIARRFSLLRKLGWSPRRTLLLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYL 420

Query: 421 NVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALN 480
           NVDCAVQGPGFFSGATPQLDDLL +VTAQV+DPDVKGATVYDTWTATNG GNIERLGALN
Sbjct: 421 NVDCAVQGPGFFSGATPQLDDLLHEVTAQVEDPDVKGATVYDTWTATNGIGNIERLGALN 480

Query: 481 SDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALR 540
           SDFAAFVQHAGVPS+DVYYGRDFPVYHTAFDTYDWMA+YGDP FHRH  VG+IWGLLALR
Sbjct: 481 SDFAAFVQHAGVPSIDVYYGRDFPVYHTAFDTYDWMASYGDPLFHRHAAVGSIWGLLALR 540

Query: 541 LADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLR 600
           LADDLILPFSYVSYANQLQAY+D LND LD SVSL  LST+I E +SAA+EIE EAKRLR
Sbjct: 541 LADDLILPFSYVSYANQLQAYRDILNDILDGSVSLQSLSTSIHEFKSAAEEIEKEAKRLR 600

Query: 601 EQETSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGI 660
           EQET + VAL Q RALNDRLMLAERGFLDVDGLRG  WFKHLVYGP SDYESAL YFPGI
Sbjct: 601 EQETFTAVALFQTRALNDRLMLAERGFLDVDGLRGHTWFKHLVYGPSSDYESALAYFPGI 660

Query: 661 ADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDLS 701
           ADA+SES KMNQ E Q  IQHEIWRVVRAI RAA ALKG+LS
Sbjct: 661 ADAISESMKMNQSEGQGVIQHEIWRVVRAITRAAAALKGELS 701

BLAST of MC05g1177 vs. TAIR 10
Match: AT3G54720.1 (Peptidase M28 family protein )

HSP 1 Score: 776.5 bits (2004), Expect = 1.7e-224
Identity = 397/705 (56.31%), Postives = 516/705 (73.19%), Query Frame = 0

Query: 3   QPPLKQLTTICTSKPAPLTTFLFIIIICLLGFYTLHFS---TSSLSATSSPRNSLRFQQL 62
           +P +  ++ I   +P PL +FLF+I++ +  FYTLH     T  L  + +  N+LR ++L
Sbjct: 8   RPTVTGISIIPFRQPPPLCSFLFVIVLFVATFYTLHHPDAVTPPLLFSRNAYNALRLRRL 67

Query: 63  FLSSASNYTVASYLRSLTLHPHLAGTEPSSETARYVESHFRDLGLETHSIQFDALLSYPK 122
           FLSSASN T++SYLR LT HPHLAGT+PS +T  YV +HF+ LGLETH  +++ALLSYP 
Sbjct: 68  FLSSASNATISSYLRELTRHPHLAGTKPSLDTLHYVFNHFQSLGLETHVAEYEALLSYPT 127

Query: 123 YASLSARLSNGSVVNIPSSE--NIQGVVKPYHAYSPSGAAYGRAVFVNYGRDEDYRALAA 182
           + S++A  SN + +    ++      VV+PYHAYSPSG+A G  VFVN+G + DY AL +
Sbjct: 128 HISVTASFSNTTTLEFDLNDVPGDSPVVRPYHAYSPSGSAQGNVVFVNHGEERDYHALES 187

Query: 183 IGVGVAGCIVVARKGD-FPRGVVVAKAEANGAKGVLLYAEGDRFR-QGFERGTVMRGIGD 242
           IGV V GC+V+ARKG+   RG +V  AEA GA GVL+YAE D     G ERGTVMRGIGD
Sbjct: 188 IGVSVKGCVVLARKGENLGRGAIVKIAEAKGALGVLIYAENDGGGFGGIERGTVMRGIGD 247

Query: 243 PLSPGWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTA 302
           P+SPGW  + G E+L+L+D  V +RFPKIPS+PLS   AEIIL+SL  A  P EWR+ + 
Sbjct: 248 PVSPGWPGVVGGEKLSLDDELVTRRFPKIPSLPLSLRNAEIILASLGGARAPLEWRN-SG 307

Query: 303 KLRSSSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAV 362
           ++     VGP G   +N T+QGE K+  I NV+  I+G EE DR+V++GNHRDAW++GAV
Sbjct: 308 RVGPGQRVGP-GRMVINMTFQGEMKMKKINNVVVTIRGSEEADRYVILGNHRDAWTYGAV 367

Query: 363 DPNSGTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKA 422
           DPNSGT+ALLDI+RRFALL + GW PRRTILLCSWDAEEFGM+GSTEW+E+N++NLG  A
Sbjct: 368 DPNSGTSALLDISRRFALLLKSGWRPRRTILLCSWDAEEFGMIGSTEWIEENVLNLGASA 427

Query: 423 VAYLNVDCAVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERL 482
           VAYLNVDCAVQG GFF+GATPQLD LL+ V   VQDPD  G TV +T+ + N    I+RL
Sbjct: 428 VAYLNVDCAVQGSGFFAGATPQLDGLLVDVLKLVQDPDAVGLTVEETFKSQNNI--IQRL 487

Query: 483 GALNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGL 542
             ++SDF+ F+ HAG+PS+D+YYG D+PVYHTAFD+YDWM +  DP FHRHV +  IWGL
Sbjct: 488 SRVDSDFSGFLHHAGIPSIDMYYGADYPVYHTAFDSYDWMIHNADPLFHRHVAMAGIWGL 547

Query: 543 LALRLADDLILPFSYVSYANQLQAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEA 602
           L + LAD+ ++PF Y+SYA+QLQA++D L+  L+  VS+ PLS AIQE    A+E  +EA
Sbjct: 548 LGILLADEPLIPFDYISYADQLQAHRDKLSKLLEGKVSVNPLSMAIQEFSLVAKEAADEA 607

Query: 603 KRLREQETSSD--VALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESAL 662
           K+L+ +  S +   A  ++R LNDRLML ERGFLD +G++G+ WFKHLVYGP ++ ES L
Sbjct: 608 KKLKGKSYSKNDVAAAAKRRELNDRLMLVERGFLDAEGIKGKEWFKHLVYGPAAEPESKL 667

Query: 663 VYFPGIADAVSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKG 699
            +FPGIADA++    MN  E    I+HEIWRV RAI+RA+ ALKG
Sbjct: 668 GFFPGIADAIA----MNASE--GIIEHEIWRVARAIQRASKALKG 702

BLAST of MC05g1177 vs. TAIR 10
Match: AT5G19740.1 (Peptidase M28 family protein )

HSP 1 Score: 553.5 bits (1425), Expect = 2.4e-157
Identity = 303/698 (43.41%), Postives = 429/698 (61.46%), Query Frame = 0

Query: 32  LGFYTLHFSTSSLSATSSPRNSLRFQQLFLSS--ASNYTVASYLRSLTLHPHLAGTEPSS 91
           L F     S S  S  SSP  S  + +LF+S+  + N +VA  L +LT  PH+AGT  ++
Sbjct: 7   LAFVIAALSYSFFSLFSSPPKS-HYHELFISTSFSDNASVALNLHTLTRRPHVAGTVANA 66

Query: 92  ETARYVESHFRDLGLETHSIQFDALLSYPKYASLSARLSNGS--VVNIPSSENI------ 151
           E A YV S F    L++H + +   L+YP + SL    ++ +  +  +   E +      
Sbjct: 67  EAAEYVRSVFTSSALKSHVVAYQVSLTYPVHRSLVLTPTDSAKPITFLLEQEKLGDNPYA 126

Query: 152 QGVVKPYHAYSPSGAAYGRAVFVNYGRDEDY-RALAAIGVGVAGCIVVARKGDFPRGVVV 211
             V+  +H Y+ SG   G  V+ NYGR ED+ R    +GV V+G +V+AR G   RG +V
Sbjct: 127 NEVMPTFHGYAKSGNVSGPVVYANYGRVEDFVRLKKDMGVNVSGAVVIARYGQIYRGDIV 186

Query: 212 AKAEANGAKGVLLYAE-----GDRF--------RQGFERGTVMRGIGDPLSPGWAAIDGA 271
             A   GA GV++Y +     GD +          G + GTV  G+GDP +PGWA++DG 
Sbjct: 187 KNAYEAGAVGVVIYTDKRDYGGDEWFPASKWMPPSGVQVGTVYNGLGDPTTPGWASVDGC 246

Query: 272 ERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSS----LDTASVPPEWRDTTAKLRSSSSV 331
           ERL+    E+    P IPS+P+SA  AE+IL +    +    V P              V
Sbjct: 247 ERLSDEAVELSGDVPLIPSLPVSAADAEVILKTVVGDVSDGDVYP--------------V 306

Query: 332 GPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAA 391
           GP GP  LN +Y GE  ++ I NV+ VI+G EEPDR+V++GNHRDAW+FGAVDPNSGTA 
Sbjct: 307 GP-GPGVLNLSYIGETVIAKIENVIGVIEGEEEPDRYVILGNHRDAWTFGAVDPNSGTAV 366

Query: 392 LLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGSTEWVEQNIVNLGTKAVAYLNVDC 451
           L++IA+R   L++ GW PRRTI+LC+WDAEE+G++GSTEWVE+N   L ++AVAYLNVDC
Sbjct: 367 LMEIAQRLDKLQKRGWKPRRTIILCNWDAEEYGLIGSTEWVEENREMLSSRAVAYLNVDC 426

Query: 452 AVQGPGFFSGATPQLDDLLLKVTAQVQDPDVKGATVYDTWTATNGFGNIERLGALNSDFA 511
           AV GPGF + ATPQLD+L+     +V+DPD    T+Y++W  ++    I RLG   SD+A
Sbjct: 427 AVSGPGFHASATPQLDELIKVAAQEVRDPDNATQTIYESWIGSSDSVVIRRLGGGGSDYA 486

Query: 512 AFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPSFHRHVTVGNIWGLLALRLADD 571
           +FVQH GVP VD+ +GR +PVYH+ +D + WM  +GDP F RHV + ++ GL+ALRLAD+
Sbjct: 487 SFVQHVGVPGVDMSFGRGYPVYHSMYDDFTWMEKFGDPMFQRHVAMASVLGLVALRLADE 546

Query: 572 LILPFSYVSYANQL-QAYKDTLNDFLDESVSLLPLSTAIQELQSAAQEIENEAKRLREQE 631
            I+PF+Y SYA +L ++ +D  N+ L  ++ +  L  +I++L +AA+ I  E + ++   
Sbjct: 547 EIIPFNYTSYALELKKSAEDLENEKLGHNIDVSTLIKSIEDLSTAAKHISLEKEAIK--- 606

Query: 632 TSSDVALLQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIADA 691
                  L+ R LNDRLM+AER   D DGL  RPW+KHL+YGP    +     FPG+ DA
Sbjct: 607 -----GALKVRELNDRLMMAERALTDRDGLSERPWYKHLIYGPSKYDDYGSKSFPGVDDA 666

Query: 692 VSESTKMNQRERQAAIQHEIWRVVRAIRRAATALKGDL 701
           +  + K+N +     +QH+IWRV RAIR A+  LKG+L
Sbjct: 667 IDNAKKLNTKASWENVQHQIWRVSRAIRHASLVLKGEL 680

BLAST of MC05g1177 vs. TAIR 10
Match: AT4G07670.1 (protease-associated (PA) domain-containing protein )

HSP 1 Score: 196.8 bits (499), Expect = 5.7e-50
Identity = 113/261 (43.30%), Postives = 154/261 (59.00%), Query Frame = 0

Query: 162 VFVNYGRDEDY-RALAAIGVGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAE---- 221
           V+ NYGR ED+ R    +GV V+G +V+AR G   +  +V  A   GA GV++Y      
Sbjct: 11  VYANYGRVEDFVRLKKDMGVNVSGAVVIARYGQIYKVDIVNNAYEAGAVGVVIYTNKRDY 70

Query: 222 -GDRF--------RQGFERGTVMRGIGDPLSPGWAAIDGAERLNLNDSEVLKRFPKIPSM 281
            GD +          GF+ GTV  G+GDP +PGWA++DG ERL+    E+    P IPS+
Sbjct: 71  GGDEWFPASKWMPPSGFQVGTVYNGLGDPTTPGWASVDGCERLSDEAVELSGDVPLIPSL 130

Query: 282 PLSAEAAEIILSSLDTASVPPEWRDTTAKLRSSSSVGPGGPTFLNFTYQGERKVSTIRNV 341
           P+SA  AE+IL ++                     VGP GP  LN +Y     V+ I+NV
Sbjct: 131 PVSAADAEVILKTI------------------VGDVGP-GPGILNLSY----IVTKIQNV 190

Query: 342 LAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLD--------IARRFALLRRLGW 401
           + VI+G EEPDR+V++ NHRD W+F AVDPNSGTA L++        IA+R   L++ GW
Sbjct: 191 IGVIEGEEEPDRYVILRNHRDTWTFRAVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGW 248

BLAST of MC05g1177 vs. TAIR 10
Match: AT4G07670.2 (protease-associated (PA) domain-containing protein )

HSP 1 Score: 192.2 bits (487), Expect = 1.4e-48
Identity = 105/236 (44.49%), Postives = 144/236 (61.02%), Query Frame = 0

Query: 178 IGVGVAGCIVVARKGDFPRGVVVAKAEANGAKGVLLYAE-----GDRF--------RQGF 237
           +GV V+G +V+AR G   +  +V  A   GA GV++Y       GD +          GF
Sbjct: 1   MGVNVSGAVVIARYGQIYKVDIVNNAYEAGAVGVVIYTNKRDYGGDEWFPASKWMPPSGF 60

Query: 238 ERGTVMRGIGDPLSPGWAAIDGAERLNLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDTA 297
           + GTV  G+GDP +PGWA++DG ERL+    E+    P IPS+P+SA  AE+IL ++   
Sbjct: 61  QVGTVYNGLGDPTTPGWASVDGCERLSDEAVELSGDVPLIPSLPVSAADAEVILKTI--- 120

Query: 298 SVPPEWRDTTAKLRSSSSVGPGGPTFLNFTYQGERKVSTIRNVLAVIKGLEEPDRFVLMG 357
                             VGP GP  LN +Y     V+ I+NV+ VI+G EEPDR+V++ 
Sbjct: 121 ---------------VGDVGP-GPGILNLSY----IVTKIQNVIGVIEGEEEPDRYVILR 180

Query: 358 NHRDAWSFGAVDPNSGTAALLDIARRFALLRRLGWSPRRTILLCSWDAEEFGMVGS 401
           NHRD W+F AVDPNSGTA L++IA+R   L++ GW PRRTI+LC+WDAEE+G+V S
Sbjct: 181 NHRDTWTFRAVDPNSGTAVLMEIAQRLDKLQKRGWKPRRTIILCNWDAEEYGLVSS 213