MC05g1086 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g1086 vs. ExPASy Swiss-Prot
Match: O22941 (Insulin-degrading enzyme-like 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=PXM16 PE=2 SV=1)

HSP 1 Score: 1459.1 bits (3776), Expect = 0.0e+00
Identity = 701/972 (72.12%), Postives = 823/972 (84.67%), Query Frame = 0

Query: 1   MAVVKEQVT---HEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSD 60
           MAV K   T    EI+KPRTD REYR IVL+N L+VLL+SDPDTDK AASM+V+VGSFSD
Sbjct: 1   MAVEKSNTTVGGVEILKPRTDNREYRMIVLKNLLQVLLISDPDTDKCAASMSVSVGSFSD 60

Query: 61  PKGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFE 120
           P+GLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+TASE+TNY+F+VNADCF+
Sbjct: 61  PQGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTASEETNYHFDVNADCFD 120

Query: 121 EALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFS 180
           EALDRFAQFFIKPLMS DATMREIKAVDSENQKNLLSDGWR+ QLQ+H+S E HPYHKFS
Sbjct: 121 EALDRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDGWRIRQLQKHLSKEDHPYHKFS 180

Query: 181 TGNWDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIP 240
           TGN DTL V+P+AKG+DTR ELI+F+E++YS+NIMHLVVY KE+LDK+Q LVE +FQ+I 
Sbjct: 181 TGNMDTLHVRPQAKGVDTRSELIKFYEEHYSANIMHLVVYGKESLDKIQDLVERMFQEIQ 240

Query: 241 NHNCNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIG 300
           N N     FPGQPCT++HL++LV+AIPIKQGHKL + WP+TP IHHY E P +YLGHLIG
Sbjct: 241 NTNKVVPRFPGQPCTADHLQILVKAIPIKQGHKLGVSWPVTPSIHHYDEAPSQYLGHLIG 300

Query: 301 HEGEGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYID 360
           HEGEGSL++ LKTLGWATGLSAGE + T ++SFFKV IDLTDAG EHMQ+I+GLLF YI 
Sbjct: 301 HEGEGSLFHALKTLGWATGLSAGEGEWTLDYSFFKVSIDLTDAGHEHMQEILGLLFNYIQ 360

Query: 361 LLKQSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKF 420
           LL+Q+G+C+WIFDELSAICETKFHYQDKI P+ Y+V+++ NMQ+YP +DWLVGSSLP+KF
Sbjct: 361 LLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLVGSSLPTKF 420

Query: 421 NPNLIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPD 480
           NP ++  V+D+LS  NFRIFWES+KFEGQTDK EPWY T YS+E I+ + IQEW+ SAPD
Sbjct: 421 NPAIVQKVVDELSPSNFRIFWESQKFEGQTDKAEPWYNTAYSLEKITSSTIQEWVQSAPD 480

Query: 481 VKLHLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRC 540
           V LHLPAPN+FIPTDLSLKDA +K   PVLLRK+ +S LWYKPDTMF  PKAYVK+DF C
Sbjct: 481 VHLHLPAPNVFIPTDLSLKDADDKETVPVLLRKTPFSRLWYKPDTMFSKPKAYVKMDFNC 540

Query: 541 PHAGISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRV 600
           P A  SP+A VLTDIFTRLLMDYLNEYAY AQVAGLYYG++ ++ GF++TL+GYNHKLR+
Sbjct: 541 PLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFELTLLGYNHKLRI 600

Query: 601 LLETVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEE 660
           LLETVV KIA F VKPDRF VIKETV KEYQN+KF+QPY QAMYYCSLILQD TWP  EE
Sbjct: 601 LLETVVGKIANFEVKPDRFAVIKETVTKEYQNYKFRQPYHQAMYYCSLILQDQTWPWTEE 660

Query: 661 LAILPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPL 720
           L +L HL AED+ KF P LLS   +ECYIAGN+E NE+ESM++HIED+ F  P PI RPL
Sbjct: 661 LDVLSHLEAEDVAKFVPMLLSRTFIECYIAGNVENNEAESMVKHIEDVLFNDPKPICRPL 720

Query: 721 YPSQHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAK 780
           +PSQH TNRVVKL  G+ YFY  +G NP+DENSALVHYIQVHRDDF  N+KLQLF LVAK
Sbjct: 721 FPSQHLTNRVVKLGEGMKYFYHQDGSNPSDENSALVHYIQVHRDDFSMNIKLQLFGLVAK 780

Query: 781 QSAFHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLV 840
           Q+ FHQLRTVEQLGYIT L QRNDSGI G+QFIIQS+ KGP +ID RVE+ L+ FESKL 
Sbjct: 781 QATFHQLRTVEQLGYITALAQRNDSGIYGVQFIIQSSVKGPGHIDSRVESLLKNFESKLY 840

Query: 841 DMTIDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQE 900
           +M+ ++FKSNV ALIDMKLEK KNL+EES FYWREI  GTLKF+R+E+EV+ L+ L  QE
Sbjct: 841 EMSNEDFKSNVTALIDMKLEKHKNLKEESRFYWREIQSGTLKFNRKEAEVSALKQLQKQE 900

Query: 901 LINFFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLY 960
           LI+FF+E+IKVGA RKK+LS+RVYG+ H  E +SD  +   P +V+I+DI  FR+SQPL+
Sbjct: 901 LIDFFDEYIKVGAARKKSLSIRVYGSQHLKEMASDKDEVPSP-SVEIEDIVGFRKSQPLH 960

Query: 961 GSFKGAFGHVKL 970
           GSF+G  G  KL
Sbjct: 961 GSFRGC-GQPKL 970

BLAST of MC05g1086 vs. ExPASy Swiss-Prot
Match: F4J3D9 (Insulin-degrading enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At3g57470 PE=3 SV=2)

HSP 1 Score: 1327.8 bits (3435), Expect = 0.0e+00
Identity = 649/966 (67.18%), Postives = 768/966 (79.50%), Query Frame = 0

Query: 1   MAVVKEQVT-----HEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSF 60
           MAV  E  T      EI+KPRTDKREYRRIVL+NSLEVLL+SDP+TDK AASM V+VGSF
Sbjct: 1   MAVGMENATASGECGEILKPRTDKREYRRIVLKNSLEVLLISDPETDKCAASMNVSVGSF 60

Query: 61  SDPKGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADC 120
           +DP+GLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+F++N D 
Sbjct: 61  TDPEGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDS 120

Query: 121 FEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHK 180
           F EALDRFAQFFI+PLMS DATMREIKAVDSE+Q NLLSD WRM QLQ+H+S E HPYHK
Sbjct: 121 FYEALDRFAQFFIQPLMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHK 180

Query: 181 FSTGNWDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQD 240
           FSTGN DTL V+P+  G+DTR ELI+F++++YS+NIMHLVVY KENLDK Q LVE +FQ 
Sbjct: 181 FSTGNMDTLHVRPEENGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQG 240

Query: 241 IPNHNCNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHL 300
           I N N     FPGQPCT +HL+VLV+A+PI QGH+L + WP+TP I HY+E PCRYLG L
Sbjct: 241 IRNTNQGIPRFPGQPCTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDL 300

Query: 301 IGHEGEGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKY 360
           IGHEGEGSL++ LK LGWATGL AGE+D +  +SFF V IDLTDAG EHMQDI+GLLF+Y
Sbjct: 301 IGHEGEGSLFHALKILGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEY 360

Query: 361 IDLLKQSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPS 420
           I +L+QSG+ +WIFDELSAICE +FHYQ KI PI Y V++S NM++YP + WLVGSSLPS
Sbjct: 361 IKVLQQSGVSQWIFDELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPS 420

Query: 421 KFNPNLIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSA 480
           KFNP ++  VLD+LS  N RIFWES KFEGQTDKVEPWY T YS+E I+   IQEWM SA
Sbjct: 421 KFNPAIVQKVLDELSPNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSA 480

Query: 481 PDVKLHLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDF 540
           PDV L LP PN+FIPTD SLKD  +K  FPVLLRK+SYS LWYKPDT FF PKAYVK+DF
Sbjct: 481 PDVNLLLPTPNVFIPTDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDF 540

Query: 541 RCPHAGISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKL 600
            CP A  SP+A VL+DIF  LL+DYLNEYAY AQ AGL YG++ ++ GF+++L G+NHKL
Sbjct: 541 NCPLAVSSPDAAVLSDIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKL 600

Query: 601 RVLLETVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLM 660
           R+LLE V++KIA+F VKPDRF VIKETV K YQN KFQQP++QA  YCSL+LQD  WP  
Sbjct: 601 RILLEAVIQKIAKFEVKPDRFSVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWT 660

Query: 661 EELAILPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISR 720
           EEL  L HL AEDL  F P LLS   +ECYIAGN+E++E+ESM++HIED+ F    PI R
Sbjct: 661 EELDALSHLEAEDLANFVPMLLSRTFVECYIAGNVEKDEAESMVKHIEDVLFTDSKPICR 720

Query: 721 PLYPSQHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALV 780
           PL+PSQ  TNRV +L  G+ +FY  EG N +DENSALVHYIQVH+D+F  N KLQLF L+
Sbjct: 721 PLFPSQFLTNRVTELGTGMKHFYYQEGSNSSDENSALVHYIQVHKDEFSMNSKLQLFELI 780

Query: 781 AKQSAFHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESK 840
           AKQ  FHQLRT+EQLGYIT L   NDSG+ G+QFIIQS+ KGP +ID RVE+ L+  ESK
Sbjct: 781 AKQDTFHQLRTIEQLGYITSLSLSNDSGVYGVQFIIQSSVKGPGHIDSRVESLLKDLESK 840

Query: 841 LVDMTIDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAH 900
             +M+ +EFKSNV  LIDMKLEK KNL EES FYW EI  GTLKF+R ++EVA LR L  
Sbjct: 841 FYNMSDEEFKSNVTNLIDMKLEKDKNLDEESWFYWAEIQTGTLKFNRIDAEVAALRLLKK 900

Query: 901 QELINFFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQP 960
            E I+FF+E+IKV AP KK+LS+ VYGN H  E  +D    +   +++I+DI  FR+SQP
Sbjct: 901 DEWIDFFDEYIKVDAPNKKSLSICVYGNQHLKEMRND-KDKIPSTSIEIEDIVCFRKSQP 960

Query: 961 LYGSFK 962
           LYGS K
Sbjct: 961 LYGSLK 965

BLAST of MC05g1086 vs. ExPASy Swiss-Prot
Match: Q24K02 (Insulin-degrading enzyme OS=Bos taurus OX=9913 GN=IDE PE=2 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 2.7e-204
Identity = 384/963 (39.88%), Postives = 555/963 (57.63%), Query Frame = 0

Query: 6    EQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKGLEGLA 65
            +++ H I+K   DKREYR + L N ++VLLVSDP TDKS+A++ V++GS SDP  + GL+
Sbjct: 48   KRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDKSSAALDVHIGSLSDPPNIAGLS 107

Query: 66   HFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEALDRFAQ 125
            HF EHMLF  ++KYP E+ YS++++EH GS NAFT+ E TNYYF+V+ +  E ALDRFAQ
Sbjct: 108  HFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQ 167

Query: 126  FFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGNWDTLE 185
            FF+ PL       RE+ AVDSE++KN+++D WR+ QL++   +  HP+ KF TGN  TLE
Sbjct: 168  FFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLE 227

Query: 186  VQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHNCNCAH 245
             +P  +G+D R EL++FH   YSSN+M + V  +E+LD + +LV  +F ++ N N     
Sbjct: 228  TRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPE 287

Query: 246  FPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLY 305
            FP  P   EHLK L + +PIK    L + +PI     +YK  P  YLGHLIGHEG GSL 
Sbjct: 288  FPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLL 347

Query: 306  YVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLKQSGIC 365
              LK+ GW   L  G+ +    F FF + +DLT+ G  H++DI+  +F+YI  L+  G  
Sbjct: 348  SELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQ 407

Query: 366  KWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPNLIGTV 425
            +W+F E   +    F ++DK RP  Y   ++  +  YP E+ L    L  +F P+LI  V
Sbjct: 408  EWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMV 467

Query: 426  LDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKLHLPAP 485
            LD+L  EN R+   SK FEG+TD+ E WYGT Y  E I   +I++W  +  + K  LP  
Sbjct: 468  LDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPMK 527

Query: 486  NIFIPTDLSLKDAH-EKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHAGISP 545
            N FIPT+  +     E   +P L++ ++ S LW+K D  FF PKA +  +F  P A + P
Sbjct: 528  NEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDP 587

Query: 546  EAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLETVVE 605
                +  ++  LL D LNEYAY+A++AGL Y +  T  G  +++ GYN K  +LL+ ++E
Sbjct: 588  LHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIE 647

Query: 606  KIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAILPHL 665
            K+A F +   RF +IKE  M+   NF+ +QP+Q AMYY  L++ +  W   E    L  +
Sbjct: 648  KMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDV 707

Query: 666  GAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPSQHPT 725
                L  F P LLS   +E  + GNI +  +  +++ +ED   +  +  ++PL PSQ   
Sbjct: 708  TLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAH--TKPLLPSQLVR 767

Query: 726  NRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSAFHQL 785
             R V+L     + Y     N    N  +  Y Q       +N+ L+LF  +  +  F+ L
Sbjct: 768  YREVQLPDRGWFVYQQR--NEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTL 827

Query: 786  RTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMTIDEF 845
            RT EQLGYI F   R  +GI+GL+FIIQS  K P  ++ RVEAFL   E  + DMT + F
Sbjct: 828  RTKEQLGYIVFSGPRRANGIQGLRFIIQS-EKPPHYLESRVEAFLITMEKSIEDMTEEAF 887

Query: 846  KSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELINFFNE 905
            + ++ AL   +L+K K L  E   YW EI      FDR   EVA L+TL  +++I F+ E
Sbjct: 888  QKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKEDIIKFYKE 947

Query: 906  HIKVGAPRKKALSVRVYGNLHSS---------EYSSDLSQ-PLQPDTVKIDDIFSFRRSQ 958
             + V APR+  +SV V      S         +   +LSQ P  P    I ++  F+R  
Sbjct: 948  MLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGL 1005

BLAST of MC05g1086 vs. ExPASy Swiss-Prot
Match: P14735 (Insulin-degrading enzyme OS=Homo sapiens OX=9606 GN=IDE PE=1 SV=4)

HSP 1 Score: 710.3 bits (1832), Expect = 3.0e-203
Identity = 382/963 (39.67%), Postives = 555/963 (57.63%), Query Frame = 0

Query: 6    EQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKGLEGLA 65
            +++ + I K   DKREYR + L N ++VLL+SDP TDKS+A++ V++GS SDP  + GL+
Sbjct: 48   KRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLS 107

Query: 66   HFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEALDRFAQ 125
            HF EHMLF  ++KYP E+ YS++++EH GS NAFT+ E TNYYF+V+ +  E ALDRFAQ
Sbjct: 108  HFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQ 167

Query: 126  FFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGNWDTLE 185
            FF+ PL       RE+ AVDSE++KN+++D WR+ QL++   +  HP+ KF TGN  TLE
Sbjct: 168  FFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLE 227

Query: 186  VQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHNCNCAH 245
             +P  +G+D R EL++FH   YSSN+M + V  +E+LD + +LV  +F ++ N N     
Sbjct: 228  TRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPE 287

Query: 246  FPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLY 305
            FP  P   EHLK L + +PIK    L + +PI     +YK  P  YLGHLIGHEG GSL 
Sbjct: 288  FPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLL 347

Query: 306  YVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLKQSGIC 365
              LK+ GW   L  G+ +    F FF + +DLT+ G  H++DI+  +F+YI  L+  G  
Sbjct: 348  SELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQ 407

Query: 366  KWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPNLIGTV 425
            +W+F E   +    F ++DK RP  Y   ++  +  YP E+ L    L  +F P+LI  V
Sbjct: 408  EWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMV 467

Query: 426  LDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKLHLPAP 485
            LD+L  EN R+   SK FEG+TD+ E WYGT Y  E I   +I++W  +  + K  LP  
Sbjct: 468  LDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTK 527

Query: 486  NIFIPTDLSLKDAH-EKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHAGISP 545
            N FIPT+  +     E   +P L++ ++ S LW+K D  FF PKA +  +F  P A + P
Sbjct: 528  NEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDP 587

Query: 546  EAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLETVVE 605
                +  ++  LL D LNEYAY+A++AGL Y +  T  G  +++ GYN K  +LL+ ++E
Sbjct: 588  LHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIE 647

Query: 606  KIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAILPHL 665
            K+A F +   RF +IKE  M+   NF+ +QP+Q AMYY  L++ +  W   E    L  +
Sbjct: 648  KMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDV 707

Query: 666  GAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPSQHPT 725
                L  F P LLS   +E  + GNI +  +  +++ +ED   +  +  ++PL PSQ   
Sbjct: 708  TLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAH--TKPLLPSQLVR 767

Query: 726  NRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSAFHQL 785
             R V+L     + Y     N    N  +  Y Q       +N+ L+LF  +  +  F+ L
Sbjct: 768  YREVQLPDRGWFVYQQR--NEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTL 827

Query: 786  RTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMTIDEF 845
            RT EQLGYI F   R  +GI+GL+FIIQS  K P  ++ RVEAFL   E  + DMT + F
Sbjct: 828  RTKEQLGYIVFSGPRRANGIQGLRFIIQS-EKPPHYLESRVEAFLITMEKSIEDMTEEAF 887

Query: 846  KSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELINFFNE 905
            + ++ AL   +L+K K L  E   YW EI      FDR  +EVA L+TL  +++I F+ E
Sbjct: 888  QKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKE 947

Query: 906  HIKVGAPRKKALSVRVYGNLHSS---------EYSSDLSQ-PLQPDTVKIDDIFSFRRSQ 958
             + V APR+  +SV V      S         +   +LSQ P  P    I ++  F+R  
Sbjct: 948  MLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGL 1005

BLAST of MC05g1086 vs. ExPASy Swiss-Prot
Match: P35559 (Insulin-degrading enzyme OS=Rattus norvegicus OX=10116 GN=Ide PE=1 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 9.6e-202
Identity = 385/966 (39.86%), Postives = 555/966 (57.45%), Query Frame = 0

Query: 6    EQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKGLEGLA 65
            +++   IVK   DKREYR + L N ++VLL+SDP TDKS+A++ V++GS SDP  + GL+
Sbjct: 48   QRIEDHIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLS 107

Query: 66   HFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEALDRFAQ 125
            HF EHMLF  ++KYP E+ YS++++EH GS NAFT+ E TNYYF+V+ +  E ALDRFAQ
Sbjct: 108  HFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQ 167

Query: 126  FFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGNWDTLE 185
            FF+ PL       RE+ AVDSE++KN+++D WR+ QL++   +  HP+ KF TGN  TLE
Sbjct: 168  FFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLE 227

Query: 186  VQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHNCNCAH 245
             +P  +G+D R EL++FH   YSSN+M + V  +E+LD + +LV  +F ++ N N     
Sbjct: 228  TRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPE 287

Query: 246  FPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLY 305
            FP  P   EHLK L + +PIK    L + +PI     +YK  P  YLGHLIGHEG GSL 
Sbjct: 288  FPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHEGPGSLL 347

Query: 306  YVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLKQSGIC 365
              LK+ GW   L  G+ +    F FF + +DLT+ G  H++DI+  +F+YI  L+  G  
Sbjct: 348  SELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQ 407

Query: 366  KWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPNLIGTV 425
            +W+F E   +    F ++DK RP  Y   ++  +  YP    L    L  +F P+LI  V
Sbjct: 408  EWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMV 467

Query: 426  LDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKLHLPAP 485
            LD+L  EN R+   SK FEG+TD+ E WYGT Y  E I   +IQ+W  +  + K  LP  
Sbjct: 468  LDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTK 527

Query: 486  NIFIPTDLSL----KDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHAG 545
            N FIPT+  +    KDA     +P L++ ++ S LW+K D  FF PKA +  +F  P A 
Sbjct: 528  NEFIPTNFEILALEKDA---TPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAY 587

Query: 546  ISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLET 605
            + P    +  ++  LL D LNEYAY+A++AGL Y +  T  G  +++ GYN K  +LL+ 
Sbjct: 588  VDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKK 647

Query: 606  VVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAIL 665
            + EK+A F +   RF +IKE  M+   NF+ +QP+Q AMYY  L++ +  W   E    L
Sbjct: 648  ITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEAL 707

Query: 666  PHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPSQ 725
              +    L  F P LLS   +E  + GNI +  +  +++ +ED   +  +  ++PL PSQ
Sbjct: 708  DDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAH--TKPLLPSQ 767

Query: 726  HPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSAF 785
                R V+L     + Y     N    N  +  Y Q       +N+ L+LF  +  +  F
Sbjct: 768  LVRYREVQLPDRGWFVYQRR--NEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCF 827

Query: 786  HQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMTI 845
            + LRT EQLGYI F   R  +GI+GL+FIIQS  K P  ++ RVEAFL   E  + DMT 
Sbjct: 828  NTLRTKEQLGYIVFSGPRRANGIQGLRFIIQS-EKPPHYLESRVEAFLITMEKAIEDMTE 887

Query: 846  DEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELINF 905
            + F+ ++ AL   +L+K K L  E   YW EI      +DR   EVA L+TL+  ++I F
Sbjct: 888  EAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNYDRDNIEVAYLKTLSKDDIIKF 947

Query: 906  FNEHIKVGAPRKKALSVRVYGNLHSS-----EYSS----DLSQ-PLQPDTVKIDDIFSFR 958
            + E + V APR+  +SV V      S     E+ S    +LS+ P  P    I ++  F+
Sbjct: 948  YKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPSQNDINLSEAPPLPQPEVIHNMTEFK 1005

BLAST of MC05g1086 vs. NCBI nr
Match: XP_022154260.1 (insulin-degrading enzyme-like 1, peroxisomal isoform X1 [Momordica charantia] >XP_022154261.1 insulin-degrading enzyme-like 1, peroxisomal isoform X1 [Momordica charantia] >XP_022154262.1 insulin-degrading enzyme-like 1, peroxisomal isoform X1 [Momordica charantia])

HSP 1 Score: 1971 bits (5107), Expect = 0.0
Identity = 968/969 (99.90%), Postives = 968/969 (99.90%), Query Frame = 0

Query: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60
           MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG
Sbjct: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60

Query: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120

Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180

Query: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240
           WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN
Sbjct: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240

Query: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300
           CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360
           EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360

Query: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420
           QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN
Sbjct: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420

Query: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480
           LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL
Sbjct: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480

Query: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540
           HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA
Sbjct: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540

Query: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600
           GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE
Sbjct: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600

Query: 601 TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660
           TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI
Sbjct: 601 TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660

Query: 661 LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720
           LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS
Sbjct: 661 LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720

Query: 721 QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780
           QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA
Sbjct: 721 QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780

Query: 781 FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840
           FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT
Sbjct: 781 FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840

Query: 841 IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900
           IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN
Sbjct: 841 IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900

Query: 901 FFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960
           FFNEHIKVGAP KKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAPHKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960

Query: 961 KGAFGHVKL 969
           KGAFGHVKL
Sbjct: 961 KGAFGHVKL 969

BLAST of MC05g1086 vs. NCBI nr
Match: KAG6580840.1 (Insulin-degrading enzyme-like 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1835 bits (4753), Expect = 0.0
Identity = 889/969 (91.74%), Postives = 936/969 (96.59%), Query Frame = 0

Query: 1    MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60
            MAV KEQVTH+IVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDP+G
Sbjct: 972  MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 1031

Query: 61   LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120
            LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN DCFEEAL
Sbjct: 1032 LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 1091

Query: 121  DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180
            DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHP+HKFSTGN
Sbjct: 1092 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 1151

Query: 181  WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240
            WDTLEVQPKAKGLDTRHEL++F++ NYSSN+MHLVVYAKENLDKVQSLVENIFQDIPN+N
Sbjct: 1152 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 1211

Query: 241  CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300
            CN A+FPGQPC SEHL+VLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 1212 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 1271

Query: 301  EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360
            EGSLYYVLKTLGWATGLSAGESDST NFSFFKV IDLTD GQEHMQD+VGLLFKYI LLK
Sbjct: 1272 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 1331

Query: 361  QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420
            +SGIC+WIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQ YPPEDWLVGSSLPSKFNPN
Sbjct: 1332 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 1391

Query: 421  LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480
            LIG VLDQLSV+N RIFWESK FEG+TDKVE WYGT YSIE ISG LIQ+W+ SAPDVKL
Sbjct: 1392 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 1451

Query: 481  HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540
            HLPA N+FIPTDLSLK A EKVEFP+LLRKSSYST+WYKPDTMFFTPKAYVKIDF+CPHA
Sbjct: 1452 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 1511

Query: 541  GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600
             ISPEAEVLT+IFTRLLMDYLNEYAYSAQVAGLYYGI+P ++GFQVTLIGYNHKLR+LLE
Sbjct: 1512 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNHKLRILLE 1571

Query: 601  TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660
            T+VEKIA F+VKPDRFLVIKETV+KEYQNFKFQ+PYQQA+YYCSLILQDHTWPLMEELAI
Sbjct: 1572 TIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWPLMEELAI 1631

Query: 661  LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720
            LPHLGAEDLVKFAPTLLSSA LECYIAGNIERNESE+MIEHIED+FFKG NPISRPLYPS
Sbjct: 1632 LPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPISRPLYPS 1691

Query: 721  QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780
            QHPTNRVVKL RGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQ+A
Sbjct: 1692 QHPTNRVVKLERGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQAA 1751

Query: 781  FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840
            FHQLRTVEQLGYIT LMQRNDSGIRG+QFIIQST KGPRNIDLRVE+FLEMFE KLVDMT
Sbjct: 1752 FHQLRTVEQLGYITVLMQRNDSGIRGVQFIIQSTVKGPRNIDLRVESFLEMFEKKLVDMT 1811

Query: 841  IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900
            I+EFKSNVNAL+DMKLEKFKNLREESGFYWREI+DGT+KFDRRESEVA L+TL HQ+LIN
Sbjct: 1812 IEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTLTHQDLIN 1871

Query: 901  FFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960
            FFNEHIKVGAPRK++LSVRVYGNLHSSEYS+DL QPLQPDTVKIDDIFSFRRSQPLYGSF
Sbjct: 1872 FFNEHIKVGAPRKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRSQPLYGSF 1931

Query: 961  KGAFGHVKL 969
            KGAFG+VKL
Sbjct: 1932 KGAFGNVKL 1940

BLAST of MC05g1086 vs. NCBI nr
Match: XP_023526970.1 (insulin-degrading enzyme-like 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1832 bits (4745), Expect = 0.0
Identity = 888/969 (91.64%), Postives = 935/969 (96.49%), Query Frame = 0

Query: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60
           MAV KEQVTH+IVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDP+G
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 60

Query: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN DCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 120

Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHP+HKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 180

Query: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240
           WDTLEVQPKAKGLDTRHEL++F++ NYSSN+MHLVVYAKENLDKVQSLVENIFQDIPN+N
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 240

Query: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300
           CN A+FPGQPC SEHL+VLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360
           EGSLYYVLKTLGWATGLSAGESDST NFSFFKV IDLTD GQEHMQD+VGLLFKYI LLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 360

Query: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420
           +SGIC+WIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQ YPPEDWLVGSSLPSKFNPN
Sbjct: 361 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 420

Query: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480
           LIG VLDQLSV+N RIFWESK FEG+TDKVE WYGT YSIE ISG LIQ+W+ SAPDVKL
Sbjct: 421 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 480

Query: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540
           HLPA N+FIPTDLSLK A EKVEFP+LLRKSSYST+WYKPDTMFFTPKAYVKIDF+CPHA
Sbjct: 481 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 540

Query: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600
            ISPEAEVLT+IFTRLLMDYLNEYAYSAQVAGLYYGI+P ++GFQVTLIGYNHKLR+LLE
Sbjct: 541 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNHKLRILLE 600

Query: 601 TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660
           T+VEKIA F+VKPDRFLVIKETV+KEYQNFKFQ+PYQQA+YYCSLILQDHTWPLMEELAI
Sbjct: 601 TIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWPLMEELAI 660

Query: 661 LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720
           LPHLGAEDLVKFAPTLLSSA LECYIAGNIERNESE+MIEHIED+FFKG NPISRPLYPS
Sbjct: 661 LPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPISRPLYPS 720

Query: 721 QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780
           QHPTNRVVKL RGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQ+A
Sbjct: 721 QHPTNRVVKLERGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQAA 780

Query: 781 FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840
           FHQLRTVEQLGYIT LMQRNDSGIRG+QFIIQST KGPRNIDLRVE+FLEMFE KLVDMT
Sbjct: 781 FHQLRTVEQLGYITVLMQRNDSGIRGVQFIIQSTVKGPRNIDLRVESFLEMFEKKLVDMT 840

Query: 841 IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900
           I+EFKSNVNAL+DMKLEKFKNLREESGFYWREI+DGT+KFDRRESEVA L+TL HQ+LIN
Sbjct: 841 IEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTLTHQDLIN 900

Query: 901 FFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960
           FFNEHIKVGA RK++LSVRVYGNLHSSEYS+DL QPLQPDTVKIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAARKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRSQPLYGSF 960

Query: 961 KGAFGHVKL 969
           KGAFG+VKL
Sbjct: 961 KGAFGNVKL 969

BLAST of MC05g1086 vs. NCBI nr
Match: XP_022934728.1 (insulin-degrading enzyme-like 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1831 bits (4743), Expect = 0.0
Identity = 887/969 (91.54%), Postives = 936/969 (96.59%), Query Frame = 0

Query: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60
           MAV KEQVTH+IVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDP+G
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 60

Query: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN DCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 120

Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHP+HKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 180

Query: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240
           WDTLEVQPKAKGLDTRHEL++F++ NYSSN+MHLVVYAKENLDKVQSLVENIFQDIPN+N
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 240

Query: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300
           CN A+FPGQPC SEHL+VLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360
           EGSLYYVLKTLGWATGLSAGESDST NFSFFKV IDLTD GQEHMQD+VGLLFKYI LLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 360

Query: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420
           +SGIC+WIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQ YPPEDWLVGSSLPSKFNPN
Sbjct: 361 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 420

Query: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480
           LIG VLDQLSV+N RIFWESK FEG+TD+VE WYGT YSIE ISG LIQ+W+ SAPDVKL
Sbjct: 421 LIGMVLDQLSVDNVRIFWESKNFEGKTDRVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 480

Query: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540
           HLPA N+FIPTDLSLK A EKVEFP+LLRKSSYST+WYKPDTMFFTPKAYVKIDF+CPHA
Sbjct: 481 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 540

Query: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600
            ISPEAEVLT+IFTRLLMDYLNEYAYSAQVAGLYYGI+P ++GFQVTLIGYNHKLR+LLE
Sbjct: 541 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNHKLRILLE 600

Query: 601 TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660
           T+VEKIA+F+VKPDRFLVIKETV+KEYQNFKFQ+PYQQA+YYCSLILQDHTWPLMEELAI
Sbjct: 601 TIVEKIAKFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWPLMEELAI 660

Query: 661 LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720
           LPHLGAEDLVKFAPTLLSSA LECYIAGNIERNESE+MIEHIED+FFKG NPISRPLYPS
Sbjct: 661 LPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPISRPLYPS 720

Query: 721 QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780
           QHPTNRVVKL RGIGYFYSAEGLN NDENSALVHYIQVHRDDFLQNVKLQLFALVAKQ+A
Sbjct: 721 QHPTNRVVKLERGIGYFYSAEGLNRNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQAA 780

Query: 781 FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840
           FHQLRTVEQLGYIT LMQRNDSGIRG+QFIIQST KGPRNIDLRVE+FLEMFE KLVDMT
Sbjct: 781 FHQLRTVEQLGYITVLMQRNDSGIRGVQFIIQSTVKGPRNIDLRVESFLEMFEKKLVDMT 840

Query: 841 IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900
           I+EFKSNVNAL+DMKLEKFKNLREESGFYWREI+DGT+KFDRRESEVA L+TL HQ+LIN
Sbjct: 841 IEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTLTHQDLIN 900

Query: 901 FFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960
           FFNEHIKVGAPRK++LSVRVYGNLHSSEYS+DL QPLQPDTVKIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAPRKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRSQPLYGSF 960

Query: 961 KGAFGHVKL 969
           KGAFG+VKL
Sbjct: 961 KGAFGNVKL 969

BLAST of MC05g1086 vs. NCBI nr
Match: XP_022982829.1 (insulin-degrading enzyme-like 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1830 bits (4740), Expect = 0.0
Identity = 886/969 (91.43%), Postives = 934/969 (96.39%), Query Frame = 0

Query: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60
           MAV KEQVTH+IVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDP+G
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 60

Query: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN DCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 120

Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHP+HKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 180

Query: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240
           WDTLEVQPKAKGLDTRHEL++F+E NYSSN+MHLVVYAKENLDKVQ LVENIFQDIPN+N
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNNN 240

Query: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300
           CN A+FPGQPC SEHL+VLVRAIPIKQGHKLRIVWPI+PEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPISPEIHHYKEGPCRYLGHLIGHEG 300

Query: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360
           EGSLYYVLKTLGWATGLSAGESDST NFSFFKV IDLTD GQEHMQD+VGLLFKYI LLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 360

Query: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420
           +SGIC+WIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQ YPPEDWLVGSSLPSKFNPN
Sbjct: 361 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 420

Query: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480
           LIG VLDQLSV+N RIFWESK FEG+TDKVE WYGT YSIE ISG LIQ+W+ SAPDVKL
Sbjct: 421 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 480

Query: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540
           HLPA N+FIPTDLSLK A EKVEFP+LLRKSSYST+WYKPDTMFFTPKAYVKIDF+CPHA
Sbjct: 481 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 540

Query: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600
            ISPEAEVLT+IFTRLLMDYLNEYAYSAQVAGLYYGI+P ++GFQVTLIGYNHKLR+LLE
Sbjct: 541 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNHKLRILLE 600

Query: 601 TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660
           T+VEKIA F+VKPDRFLVIKETV+KEYQNFKFQ+PYQQA+YYCSLILQDHTWPLMEELAI
Sbjct: 601 TIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWPLMEELAI 660

Query: 661 LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720
           LPHLGAEDLVKFAPTLLSSA LECYIAGNIERNESE+MIEHIED+FFKG NPISRPLYPS
Sbjct: 661 LPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPISRPLYPS 720

Query: 721 QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780
           QHPTNRVVKL RGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQ+A
Sbjct: 721 QHPTNRVVKLERGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQAA 780

Query: 781 FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840
           FHQLRTVEQLGYIT LMQRND GIRG+QF+IQST KGPRNIDLRVE+FLEMFE KLVDMT
Sbjct: 781 FHQLRTVEQLGYITVLMQRNDFGIRGVQFVIQSTVKGPRNIDLRVESFLEMFEKKLVDMT 840

Query: 841 IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900
           I+EFKSNVNAL+DMKLEKFKNLREESGFYWREI+DGT+KFDRRESEVA L+TL HQ+LIN
Sbjct: 841 IEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTLTHQDLIN 900

Query: 901 FFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960
           FFNEHIKVGAPRK++LSVRVYGNLHSSEYS+DL QPLQPDTVKIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAPRKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRSQPLYGSF 960

Query: 961 KGAFGHVKL 969
           KGAFG+VKL
Sbjct: 961 KGAFGNVKL 969

BLAST of MC05g1086 vs. ExPASy TrEMBL
Match: A0A6J1DJ48 (insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021555 PE=3 SV=1)

HSP 1 Score: 1971 bits (5107), Expect = 0.0
Identity = 968/969 (99.90%), Postives = 968/969 (99.90%), Query Frame = 0

Query: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60
           MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG
Sbjct: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60

Query: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120

Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180

Query: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240
           WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN
Sbjct: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240

Query: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300
           CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360
           EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360

Query: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420
           QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN
Sbjct: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420

Query: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480
           LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL
Sbjct: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480

Query: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540
           HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA
Sbjct: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540

Query: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600
           GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE
Sbjct: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600

Query: 601 TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660
           TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI
Sbjct: 601 TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660

Query: 661 LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720
           LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS
Sbjct: 661 LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720

Query: 721 QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780
           QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA
Sbjct: 721 QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780

Query: 781 FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840
           FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT
Sbjct: 781 FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840

Query: 841 IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900
           IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN
Sbjct: 841 IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900

Query: 901 FFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960
           FFNEHIKVGAP KKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAPHKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960

Query: 961 KGAFGHVKL 969
           KGAFGHVKL
Sbjct: 961 KGAFGHVKL 969

BLAST of MC05g1086 vs. ExPASy TrEMBL
Match: A0A6J1F8G9 (insulin-degrading enzyme-like 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111441824 PE=3 SV=1)

HSP 1 Score: 1831 bits (4743), Expect = 0.0
Identity = 887/969 (91.54%), Postives = 936/969 (96.59%), Query Frame = 0

Query: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60
           MAV KEQVTH+IVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDP+G
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 60

Query: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN DCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 120

Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHP+HKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 180

Query: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240
           WDTLEVQPKAKGLDTRHEL++F++ NYSSN+MHLVVYAKENLDKVQSLVENIFQDIPN+N
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIFQDIPNNN 240

Query: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300
           CN A+FPGQPC SEHL+VLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360
           EGSLYYVLKTLGWATGLSAGESDST NFSFFKV IDLTD GQEHMQD+VGLLFKYI LLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 360

Query: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420
           +SGIC+WIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQ YPPEDWLVGSSLPSKFNPN
Sbjct: 361 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 420

Query: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480
           LIG VLDQLSV+N RIFWESK FEG+TD+VE WYGT YSIE ISG LIQ+W+ SAPDVKL
Sbjct: 421 LIGMVLDQLSVDNVRIFWESKNFEGKTDRVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 480

Query: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540
           HLPA N+FIPTDLSLK A EKVEFP+LLRKSSYST+WYKPDTMFFTPKAYVKIDF+CPHA
Sbjct: 481 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 540

Query: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600
            ISPEAEVLT+IFTRLLMDYLNEYAYSAQVAGLYYGI+P ++GFQVTLIGYNHKLR+LLE
Sbjct: 541 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNHKLRILLE 600

Query: 601 TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660
           T+VEKIA+F+VKPDRFLVIKETV+KEYQNFKFQ+PYQQA+YYCSLILQDHTWPLMEELAI
Sbjct: 601 TIVEKIAKFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWPLMEELAI 660

Query: 661 LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720
           LPHLGAEDLVKFAPTLLSSA LECYIAGNIERNESE+MIEHIED+FFKG NPISRPLYPS
Sbjct: 661 LPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPISRPLYPS 720

Query: 721 QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780
           QHPTNRVVKL RGIGYFYSAEGLN NDENSALVHYIQVHRDDFLQNVKLQLFALVAKQ+A
Sbjct: 721 QHPTNRVVKLERGIGYFYSAEGLNRNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQAA 780

Query: 781 FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840
           FHQLRTVEQLGYIT LMQRNDSGIRG+QFIIQST KGPRNIDLRVE+FLEMFE KLVDMT
Sbjct: 781 FHQLRTVEQLGYITVLMQRNDSGIRGVQFIIQSTVKGPRNIDLRVESFLEMFEKKLVDMT 840

Query: 841 IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900
           I+EFKSNVNAL+DMKLEKFKNLREESGFYWREI+DGT+KFDRRESEVA L+TL HQ+LIN
Sbjct: 841 IEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTLTHQDLIN 900

Query: 901 FFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960
           FFNEHIKVGAPRK++LSVRVYGNLHSSEYS+DL QPLQPDTVKIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAPRKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRSQPLYGSF 960

Query: 961 KGAFGHVKL 969
           KGAFG+VKL
Sbjct: 961 KGAFGNVKL 969

BLAST of MC05g1086 vs. ExPASy TrEMBL
Match: A0A6J1J5M2 (insulin-degrading enzyme-like 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111481567 PE=3 SV=1)

HSP 1 Score: 1830 bits (4740), Expect = 0.0
Identity = 886/969 (91.43%), Postives = 934/969 (96.39%), Query Frame = 0

Query: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60
           MAV KEQVTH+IVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDP+G
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPEG 60

Query: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120
           LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN DCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNVDCFEEAL 120

Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHP+HKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPFHKFSTGN 180

Query: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240
           WDTLEVQPKAKGLDTRHEL++F+E NYSSN+MHLVVYAKENLDKVQ LVENIFQDIPN+N
Sbjct: 181 WDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNNN 240

Query: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300
           CN A+FPGQPC SEHL+VLVRAIPIKQGHKLRIVWPI+PEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPISPEIHHYKEGPCRYLGHLIGHEG 300

Query: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360
           EGSLYYVLKTLGWATGLSAGESDST NFSFFKV IDLTD GQEHMQD+VGLLFKYI LLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLFKYISLLK 360

Query: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420
           +SGIC+WIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQ YPPEDWLVGSSLPSKFNPN
Sbjct: 361 ESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSLPSKFNPN 420

Query: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480
           LIG VLDQLSV+N RIFWESK FEG+TDKVE WYGT YSIE ISG LIQ+W+ SAPDVKL
Sbjct: 421 LIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQSAPDVKL 480

Query: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540
           HLPA N+FIPTDLSLK A EKVEFP+LLRKSSYST+WYKPDTMFFTPKAYVKIDF+CPHA
Sbjct: 481 HLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKIDFKCPHA 540

Query: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600
            ISPEAEVLT+IFTRLLMDYLNEYAYSAQVAGLYYGI+P ++GFQVTLIGYNHKLR+LLE
Sbjct: 541 DISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNHKLRILLE 600

Query: 601 TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660
           T+VEKIA F+VKPDRFLVIKETV+KEYQNFKFQ+PYQQA+YYCSLILQDHTWPLMEELAI
Sbjct: 601 TIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWPLMEELAI 660

Query: 661 LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720
           LPHLGAEDLVKFAPTLLSSA LECYIAGNIERNESE+MIEHIED+FFKG NPISRPLYPS
Sbjct: 661 LPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPISRPLYPS 720

Query: 721 QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780
           QHPTNRVVKL RGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQ+A
Sbjct: 721 QHPTNRVVKLERGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQAA 780

Query: 781 FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840
           FHQLRTVEQLGYIT LMQRND GIRG+QF+IQST KGPRNIDLRVE+FLEMFE KLVDMT
Sbjct: 781 FHQLRTVEQLGYITVLMQRNDFGIRGVQFVIQSTVKGPRNIDLRVESFLEMFEKKLVDMT 840

Query: 841 IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900
           I+EFKSNVNAL+DMKLEKFKNLREESGFYWREI+DGT+KFDRRESEVA L+TL HQ+LIN
Sbjct: 841 IEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTLTHQDLIN 900

Query: 901 FFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960
           FFNEHIKVGAPRK++LSVRVYGNLHSSEYS+DL QPLQPDTVKIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAPRKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRSQPLYGSF 960

Query: 961 KGAFGHVKL 969
           KGAFG+VKL
Sbjct: 961 KGAFGNVKL 969

BLAST of MC05g1086 vs. ExPASy TrEMBL
Match: A0A6J1DJV0 (insulin-degrading enzyme-like 1, peroxisomal isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021555 PE=4 SV=1)

HSP 1 Score: 1786 bits (4627), Expect = 0.0
Identity = 873/874 (99.89%), Postives = 873/874 (99.89%), Query Frame = 0

Query: 96  MNAFTASEQTNYYFEVNADCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSD 155
           MNAFTASEQTNYYFEVNADCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSD
Sbjct: 1   MNAFTASEQTNYYFEVNADCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSD 60

Query: 156 GWRMHQLQRHISSESHPYHKFSTGNWDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLV 215
           GWRMHQLQRHISSESHPYHKFSTGNWDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLV
Sbjct: 61  GWRMHQLQRHISSESHPYHKFSTGNWDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLV 120

Query: 216 VYAKENLDKVQSLVENIFQDIPNHNCNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVW 275
           VYAKENLDKVQSLVENIFQDIPNHNCNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVW
Sbjct: 121 VYAKENLDKVQSLVENIFQDIPNHNCNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVW 180

Query: 276 PITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVII 335
           PITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVII
Sbjct: 181 PITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVII 240

Query: 336 DLTDAGQEHMQDIVGLLFKYIDLLKQSGICKWIFDELSAICETKFHYQDKIRPIDYVVNL 395
           DLTDAGQEHMQDIVGLLFKYIDLLKQSGICKWIFDELSAICETKFHYQDKIRPIDYVVNL
Sbjct: 241 DLTDAGQEHMQDIVGLLFKYIDLLKQSGICKWIFDELSAICETKFHYQDKIRPIDYVVNL 300

Query: 396 SLNMQLYPPEDWLVGSSLPSKFNPNLIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYG 455
           SLNMQLYPPEDWLVGSSLPSKFNPNLIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYG
Sbjct: 301 SLNMQLYPPEDWLVGSSLPSKFNPNLIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYG 360

Query: 456 TTYSIENISGTLIQEWMLSAPDVKLHLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYST 515
           TTYSIENISGTLIQEWMLSAPDVKLHLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYST
Sbjct: 361 TTYSIENISGTLIQEWMLSAPDVKLHLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYST 420

Query: 516 LWYKPDTMFFTPKAYVKIDFRCPHAGISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYY 575
           LWYKPDTMFFTPKAYVKIDFRCPHAGISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYY
Sbjct: 421 LWYKPDTMFFTPKAYVKIDFRCPHAGISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYY 480

Query: 576 GINPTEAGFQVTLIGYNHKLRVLLETVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQP 635
           GINPTEAGFQVTLIGYNHKLRVLLETVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQP
Sbjct: 481 GINPTEAGFQVTLIGYNHKLRVLLETVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQP 540

Query: 636 YQQAMYYCSLILQDHTWPLMEELAILPHLGAEDLVKFAPTLLSSACLECYIAGNIERNES 695
           YQQAMYYCSLILQDHTWPLMEELAILPHLGAEDLVKFAPTLLSSACLECYIAGNIERNES
Sbjct: 541 YQQAMYYCSLILQDHTWPLMEELAILPHLGAEDLVKFAPTLLSSACLECYIAGNIERNES 600

Query: 696 ESMIEHIEDIFFKGPNPISRPLYPSQHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHY 755
           ESMIEHIEDIFFKGPNPISRPLYPSQHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHY
Sbjct: 601 ESMIEHIEDIFFKGPNPISRPLYPSQHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHY 660

Query: 756 IQVHRDDFLQNVKLQLFALVAKQSAFHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTA 815
           IQVHRDDFLQNVKLQLFALVAKQSAFHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTA
Sbjct: 661 IQVHRDDFLQNVKLQLFALVAKQSAFHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTA 720

Query: 816 KGPRNIDLRVEAFLEMFESKLVDMTIDEFKSNVNALIDMKLEKFKNLREESGFYWREISD 875
           KGPRNIDLRVEAFLEMFESKLVDMTIDEFKSNVNALIDMKLEKFKNLREESGFYWREISD
Sbjct: 721 KGPRNIDLRVEAFLEMFESKLVDMTIDEFKSNVNALIDMKLEKFKNLREESGFYWREISD 780

Query: 876 GTLKFDRRESEVATLRTLAHQELINFFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQ 935
           GTLKFDRRESEVATLRTLAHQELINFFNEHIKVGAP KKALSVRVYGNLHSSEYSSDLSQ
Sbjct: 781 GTLKFDRRESEVATLRTLAHQELINFFNEHIKVGAPHKKALSVRVYGNLHSSEYSSDLSQ 840

Query: 936 PLQPDTVKIDDIFSFRRSQPLYGSFKGAFGHVKL 969
           PLQPDTVKIDDIFSFRRSQPLYGSFKGAFGHVKL
Sbjct: 841 PLQPDTVKIDDIFSFRRSQPLYGSFKGAFGHVKL 874

BLAST of MC05g1086 vs. ExPASy TrEMBL
Match: A0A1S3B7B5 (insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486819 PE=3 SV=1)

HSP 1 Score: 1743 bits (4515), Expect = 0.0
Identity = 846/969 (87.31%), Postives = 909/969 (93.81%), Query Frame = 0

Query: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSDPKG 60
           MAV KEQVTHEIVKPR DKREYRRIVL+NSLEVLLVSDPDTDKSAASMTV+VGSFSDP+G
Sbjct: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLKNSLEVLLVSDPDTDKSAASMTVDVGSFSDPEG 60

Query: 61  LEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEAL 120
           LEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGSMNAFTASE+TNYYF+VNADCFEEAL
Sbjct: 61  LEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSMNAFTASEETNYYFDVNADCFEEAL 120

Query: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGN 180
           DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQ HISSESHP+HKFSTGN
Sbjct: 121 DRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQSHISSESHPFHKFSTGN 180

Query: 181 WDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHN 240
           W+TLEVQPKAKGLDTRHEL++F+E NYSSN+MHLVVYAKE LDKVQ LVENIFQDIPNHN
Sbjct: 181 WETLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKEKLDKVQILVENIFQDIPNHN 240

Query: 241 CNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEG 300
           CN A+FPGQPCTSEHL+VLVRAIPIK+GHKLRI+WPITPEIHHYKEGPCRYLGHLIGHEG
Sbjct: 241 CNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHYKEGPCRYLGHLIGHEG 300

Query: 301 EGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLK 360
           EGSLYYVLKTLGWATGLSAGESDST NFSFFKV+IDLTD GQEHMQ+++GLLF+YI LLK
Sbjct: 301 EGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQEVIGLLFRYISLLK 360

Query: 361 QSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPN 420
           QSGIC+WIFDELSAICETKFHY DKIRPI+YV++LSLNMQLYPPEDWLVGSSLPSKF+P 
Sbjct: 361 QSGICQWIFDELSAICETKFHYADKIRPINYVISLSLNMQLYPPEDWLVGSSLPSKFDPK 420

Query: 421 LIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKL 480
           LIGTVLDQLSV+N RIFWESKKFEG+TDKVE WYGT YSIE ISG L+Q+WM SAPDV L
Sbjct: 421 LIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKISGHLVQDWMQSAPDVNL 480

Query: 481 HLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHA 540
           HLPA NIFIPTDLSLK A EKVEFPVLLRKSSYS++WYKPDTMF TPKAYVKIDF CPHA
Sbjct: 481 HLPASNIFIPTDLSLKRACEKVEFPVLLRKSSYSSIWYKPDTMFSTPKAYVKIDFICPHA 540

Query: 541 GISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLE 600
            ISPEAEVLT IFTRLLMDYLNEYAY A+VAGLYYGIN  ++GFQVTL GYNHKLRVLLE
Sbjct: 541 DISPEAEVLTAIFTRLLMDYLNEYAYYAEVAGLYYGINLIDSGFQVTLNGYNHKLRVLLE 600

Query: 601 TVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAI 660
           T+VEKIA+F+VKPDR+LVIKET++K+YQNFKFQQPYQQA+YYCSLIL+D TWPLM+ELAI
Sbjct: 601 TIVEKIAKFSVKPDRYLVIKETLLKDYQNFKFQQPYQQALYYCSLILEDRTWPLMDELAI 660

Query: 661 LPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPS 720
           LPHLGA DL KF PTLLSSA LEC+IAGNIER E+ESMI+HIED FFKGPNPISRPL PS
Sbjct: 661 LPHLGARDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDTFFKGPNPISRPLNPS 720

Query: 721 QHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSA 780
           Q+P NR+VKL RGIGY YSAEGLN NDENSALVHYIQVHRD+FLQNVKLQLFA VAKQ+A
Sbjct: 721 QYPANRIVKLERGIGYLYSAEGLNSNDENSALVHYIQVHRDEFLQNVKLQLFAHVAKQAA 780

Query: 781 FHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMT 840
           FHQLRTVEQLGYIT L Q N  GIRG+QFIIQST KGPR IDLRVEAFL+MFE KLV+MT
Sbjct: 781 FHQLRTVEQLGYITVLAQSNHCGIRGVQFIIQSTVKGPRGIDLRVEAFLDMFEKKLVEMT 840

Query: 841 IDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELIN 900
           IDEFKSNVNAL+D KLEKFKNL+EES FYW EIS GTLKFDRRESEVA L+TL HQ+LIN
Sbjct: 841 IDEFKSNVNALVDAKLEKFKNLKEESRFYWGEISGGTLKFDRRESEVALLKTLTHQDLIN 900

Query: 901 FFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSF 960
           FFNEHIKVGAP KK+LSVRVYGNLHSSEYS DL+QPLQP+TVKIDDIFSFRRSQPLYGSF
Sbjct: 901 FFNEHIKVGAPCKKSLSVRVYGNLHSSEYSGDLNQPLQPNTVKIDDIFSFRRSQPLYGSF 960

Query: 961 KGAFGHVKL 969
           KGA+G+VKL
Sbjct: 961 KGAYGNVKL 969

BLAST of MC05g1086 vs. TAIR 10
Match: AT2G41790.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 1459.1 bits (3776), Expect = 0.0e+00
Identity = 701/972 (72.12%), Postives = 823/972 (84.67%), Query Frame = 0

Query: 1   MAVVKEQVT---HEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSFSD 60
           MAV K   T    EI+KPRTD REYR IVL+N L+VLL+SDPDTDK AASM+V+VGSFSD
Sbjct: 1   MAVEKSNTTVGGVEILKPRTDNREYRMIVLKNLLQVLLISDPDTDKCAASMSVSVGSFSD 60

Query: 61  PKGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFE 120
           P+GLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+TASE+TNY+F+VNADCF+
Sbjct: 61  PQGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTASEETNYHFDVNADCFD 120

Query: 121 EALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFS 180
           EALDRFAQFFIKPLMS DATMREIKAVDSENQKNLLSDGWR+ QLQ+H+S E HPYHKFS
Sbjct: 121 EALDRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDGWRIRQLQKHLSKEDHPYHKFS 180

Query: 181 TGNWDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIP 240
           TGN DTL V+P+AKG+DTR ELI+F+E++YS+NIMHLVVY KE+LDK+Q LVE +FQ+I 
Sbjct: 181 TGNMDTLHVRPQAKGVDTRSELIKFYEEHYSANIMHLVVYGKESLDKIQDLVERMFQEIQ 240

Query: 241 NHNCNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIG 300
           N N     FPGQPCT++HL++LV+AIPIKQGHKL + WP+TP IHHY E P +YLGHLIG
Sbjct: 241 NTNKVVPRFPGQPCTADHLQILVKAIPIKQGHKLGVSWPVTPSIHHYDEAPSQYLGHLIG 300

Query: 301 HEGEGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYID 360
           HEGEGSL++ LKTLGWATGLSAGE + T ++SFFKV IDLTDAG EHMQ+I+GLLF YI 
Sbjct: 301 HEGEGSLFHALKTLGWATGLSAGEGEWTLDYSFFKVSIDLTDAGHEHMQEILGLLFNYIQ 360

Query: 361 LLKQSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKF 420
           LL+Q+G+C+WIFDELSAICETKFHYQDKI P+ Y+V+++ NMQ+YP +DWLVGSSLP+KF
Sbjct: 361 LLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLVGSSLPTKF 420

Query: 421 NPNLIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPD 480
           NP ++  V+D+LS  NFRIFWES+KFEGQTDK EPWY T YS+E I+ + IQEW+ SAPD
Sbjct: 421 NPAIVQKVVDELSPSNFRIFWESQKFEGQTDKAEPWYNTAYSLEKITSSTIQEWVQSAPD 480

Query: 481 VKLHLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRC 540
           V LHLPAPN+FIPTDLSLKDA +K   PVLLRK+ +S LWYKPDTMF  PKAYVK+DF C
Sbjct: 481 VHLHLPAPNVFIPTDLSLKDADDKETVPVLLRKTPFSRLWYKPDTMFSKPKAYVKMDFNC 540

Query: 541 PHAGISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRV 600
           P A  SP+A VLTDIFTRLLMDYLNEYAY AQVAGLYYG++ ++ GF++TL+GYNHKLR+
Sbjct: 541 PLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFELTLLGYNHKLRI 600

Query: 601 LLETVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEE 660
           LLETVV KIA F VKPDRF VIKETV KEYQN+KF+QPY QAMYYCSLILQD TWP  EE
Sbjct: 601 LLETVVGKIANFEVKPDRFAVIKETVTKEYQNYKFRQPYHQAMYYCSLILQDQTWPWTEE 660

Query: 661 LAILPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPL 720
           L +L HL AED+ KF P LLS   +ECYIAGN+E NE+ESM++HIED+ F  P PI RPL
Sbjct: 661 LDVLSHLEAEDVAKFVPMLLSRTFIECYIAGNVENNEAESMVKHIEDVLFNDPKPICRPL 720

Query: 721 YPSQHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAK 780
           +PSQH TNRVVKL  G+ YFY  +G NP+DENSALVHYIQVHRDDF  N+KLQLF LVAK
Sbjct: 721 FPSQHLTNRVVKLGEGMKYFYHQDGSNPSDENSALVHYIQVHRDDFSMNIKLQLFGLVAK 780

Query: 781 QSAFHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLV 840
           Q+ FHQLRTVEQLGYIT L QRNDSGI G+QFIIQS+ KGP +ID RVE+ L+ FESKL 
Sbjct: 781 QATFHQLRTVEQLGYITALAQRNDSGIYGVQFIIQSSVKGPGHIDSRVESLLKNFESKLY 840

Query: 841 DMTIDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQE 900
           +M+ ++FKSNV ALIDMKLEK KNL+EES FYWREI  GTLKF+R+E+EV+ L+ L  QE
Sbjct: 841 EMSNEDFKSNVTALIDMKLEKHKNLKEESRFYWREIQSGTLKFNRKEAEVSALKQLQKQE 900

Query: 901 LINFFNEHIKVGAPRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLY 960
           LI+FF+E+IKVGA RKK+LS+RVYG+ H  E +SD  +   P +V+I+DI  FR+SQPL+
Sbjct: 901 LIDFFDEYIKVGAARKKSLSIRVYGSQHLKEMASDKDEVPSP-SVEIEDIVGFRKSQPLH 960

Query: 961 GSFKGAFGHVKL 970
           GSF+G  G  KL
Sbjct: 961 GSFRGC-GQPKL 970

BLAST of MC05g1086 vs. TAIR 10
Match: AT3G57470.2 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 593/891 (66.55%), Postives = 705/891 (79.12%), Query Frame = 0

Query: 71  MLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEALDRFAQFFIKP 130
           MLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+F++N D F EALDRFAQFFI+P
Sbjct: 1   MLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDSFYEALDRFAQFFIQP 60

Query: 131 LMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGNWDTLEVQPKA 190
           LMS DATMREIKAVDSE+Q NLLSD WRM QLQ+H+S E HPYHKFSTGN DTL V+P+ 
Sbjct: 61  LMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHKFSTGNMDTLHVRPEE 120

Query: 191 KGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHNCNCAHFPGQP 250
            G+DTR ELI+F++++YS+NIMHLVVY KENLDK Q LVE +FQ I N N     FPGQP
Sbjct: 121 NGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQGIRNTNQGIPRFPGQP 180

Query: 251 CTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKT 310
           CT +HL+VLV+A+PI QGH+L + WP+TP I HY+E PCRYLG LIGHEGEGSL++ LK 
Sbjct: 181 CTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDLIGHEGEGSLFHALKI 240

Query: 311 LGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLKQSGICKWIFD 370
           LGWATGL AGE+D +  +SFF V IDLTDAG EHMQDI+GLLF+YI +L+QSG+ +WIFD
Sbjct: 241 LGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEYIKVLQQSGVSQWIFD 300

Query: 371 ELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPNLIGTVLDQLS 430
           ELSAICE +FHYQ KI PI Y V++S NM++YP + WLVGSSLPSKFNP ++  VLD+LS
Sbjct: 301 ELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAIVQKVLDELS 360

Query: 431 VENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKLHLPAPNIFIP 490
             N RIFWES KFEGQTDKVEPWY T YS+E I+   IQEWM SAPDV L LP PN+FIP
Sbjct: 361 PNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSAPDVNLLLPTPNVFIP 420

Query: 491 TDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHAGISPEAEVLT 550
           TD SLKD  +K  FPVLLRK+SYS LWYKPDT FF PKAYVK+DF CP A  SP+A VL+
Sbjct: 421 TDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDFNCPLAVSSPDAAVLS 480

Query: 551 DIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLETVVEKIARFN 610
           DIF  LL+DYLNEYAY AQ AGL YG++ ++ GF+++L G+NHKLR+LLE V++KIA+F 
Sbjct: 481 DIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKLRILLEAVIQKIAKFE 540

Query: 611 VKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAILPHLGAEDLV 670
           VKPDRF VIKETV K YQN KFQQP++QA  YCSL+LQD  WP  EEL  L HL AEDL 
Sbjct: 541 VKPDRFSVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWTEELDALSHLEAEDLA 600

Query: 671 KFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPSQHPTNRVVKL 730
            F P LLS   +ECYIAGN+E++E+ESM++HIED+ F    PI RPL+PSQ  TNRV +L
Sbjct: 601 NFVPMLLSRTFVECYIAGNVEKDEAESMVKHIEDVLFTDSKPICRPLFPSQFLTNRVTEL 660

Query: 731 ARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSAFHQLRTVEQL 790
             G+ +FY  EG N +DENSALVHYIQVH+D+F  N KLQLF L+AKQ  FHQLRT+EQL
Sbjct: 661 GTGMKHFYYQEGSNSSDENSALVHYIQVHKDEFSMNSKLQLFELIAKQDTFHQLRTIEQL 720

Query: 791 GYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMTIDEFKSNVNA 850
           GYIT L   NDSG+ G+QFIIQS+ KGP +ID RVE+ L+  ESK  +M+ +EFKSNV  
Sbjct: 721 GYITSLSLSNDSGVYGVQFIIQSSVKGPGHIDSRVESLLKDLESKFYNMSDEEFKSNVTN 780

Query: 851 LIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELINFFNEHIKVGA 910
           LIDMKLEK KNL EES FYW EI  GTLKF+R ++EVA LR L   E I+FF+E+IKV A
Sbjct: 781 LIDMKLEKDKNLDEESWFYWAEIQTGTLKFNRIDAEVAALRLLKKDEWIDFFDEYIKVDA 840

Query: 911 PRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSFK 962
           P KK+LS+ VYGN H  E  +D    +   +++I+DI  FR+SQPLYGS K
Sbjct: 841 PNKKSLSICVYGNQHLKEMRND-KDKIPSTSIEIEDIVCFRKSQPLYGSLK 890

BLAST of MC05g1086 vs. TAIR 10
Match: AT3G57470.3 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 1203.3 bits (3112), Expect = 0.0e+00
Identity = 585/891 (65.66%), Postives = 697/891 (78.23%), Query Frame = 0

Query: 71  MLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEALDRFAQFFIKP 130
           MLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+F++N D F EALDRFAQFFI+P
Sbjct: 1   MLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDSFYEALDRFAQFFIQP 60

Query: 131 LMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHKFSTGNWDTLEVQPKA 190
           LMS DATMREIKAVDSE+Q NLLSD WRM QLQ+H+S E HPYHKFSTGN DTL V+P+ 
Sbjct: 61  LMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHKFSTGNMDTLHVRPEE 120

Query: 191 KGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQDIPNHNCNCAHFPGQP 250
            G+DTR ELI+F++++YS+NIMHLVVY KENLDK Q LVE +FQ I N N     FPGQP
Sbjct: 121 NGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQGIRNTNQGIPRFPGQP 180

Query: 251 CTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKT 310
           CT +HL+VLV+A+PI QGH+L + WP+TP I HY+E PCRYLG LIGHEGEGSL++ LK 
Sbjct: 181 CTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDLIGHEGEGSLFHALKI 240

Query: 311 LGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKYIDLLKQSGICKWIFD 370
           LGWATGL AGE+D +  +SFF V IDLTDAG EHMQDI+GLLF+YI +L+QSG+ +WIFD
Sbjct: 241 LGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEYIKVLQQSGVSQWIFD 300

Query: 371 ELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPNLIGTVLDQLS 430
           ELSAICE +FHYQ KI PI Y V++S NM++YP + WLVGSSLPSKFNP ++  VLD+LS
Sbjct: 301 ELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAIVQKVLDELS 360

Query: 431 VENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSAPDVKLHLPAPNIFIP 490
             N RIFWES KFEGQTDKVEPWY T YS+E I+   IQEWM SAPDV L LP PN+FIP
Sbjct: 361 PNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSAPDVNLLLPTPNVFIP 420

Query: 491 TDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDFRCPHAGISPEAEVLT 550
           TD SLKD  +K  FPVLLRK+SYS LWYKPDT FF PKAYVK+DF CP A  SP+A VL+
Sbjct: 421 TDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDFNCPLAVSSPDAAVLS 480

Query: 551 DIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLETVVEKIARFN 610
           DIF  LL+DYLNEYAY AQ AGL YG++ ++ GF+++L G+NHKLR+LLE V++KIA+F 
Sbjct: 481 DIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKLRILLEAVIQKIAKFE 540

Query: 611 VKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAILPHLGAEDLV 670
           VKPDRF VIKETV K YQN KFQQP++QA  YCSL+LQD  WP  EEL  L HL AEDL 
Sbjct: 541 VKPDRFSVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWTEELDALSHLEAEDLA 600

Query: 671 KFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPSQHPTNRVVKL 730
            F P LLS   +ECYIAGN+E++E+ESM++HIED+ F    PI RPL+PSQ  TNRV +L
Sbjct: 601 NFVPMLLSRTFVECYIAGNVEKDEAESMVKHIEDVLFTDSKPICRPLFPSQFLTNRVTEL 660

Query: 731 ARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALVAKQSAFHQLRTVEQL 790
             G+ +FY  EG N +DENSALVHYIQVH+D+F  N KLQLF L+AKQ  FHQLRT+EQL
Sbjct: 661 GTGMKHFYYQEGSNSSDENSALVHYIQVHKDEFSMNSKLQLFELIAKQDTFHQLRTIEQL 720

Query: 791 GYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMTIDEFKSNVNA 850
           GYIT L   NDSG+ G+QFIIQS+ KGP +ID RVE+ L+  ESK  +M+ +EF      
Sbjct: 721 GYITSLSLSNDSGVYGVQFIIQSSVKGPGHIDSRVESLLKDLESKFYNMSDEEF------ 780

Query: 851 LIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELINFFNEHIKVGA 910
               KLEK KNL EES FYW EI  GTLKF+R ++EVA LR L   E I+FF+E+IKV A
Sbjct: 781 ----KLEKDKNLDEESWFYWAEIQTGTLKFNRIDAEVAALRLLKKDEWIDFFDEYIKVDA 840

Query: 911 PRKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGSFK 962
           P KK+LS+ VYGN H  E  +D    +   +++I+DI  FR+SQPLYGS K
Sbjct: 841 PNKKSLSICVYGNQHLKEMRND-KDKIPSTSIEIEDIVCFRKSQPLYGSLK 880

BLAST of MC05g1086 vs. TAIR 10
Match: AT3G57470.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 1201.0 bits (3106), Expect = 0.0e+00
Identity = 581/850 (68.35%), Postives = 685/850 (80.59%), Query Frame = 0

Query: 1   MAVVKEQVT-----HEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDKSAASMTVNVGSF 60
           MAV  E  T      EI+KPRTDKREYRRIVL+NSLEVLL+SDP+TDK AASM V+VGSF
Sbjct: 1   MAVGMENATASGECGEILKPRTDKREYRRIVLKNSLEVLLISDPETDKCAASMNVSVGSF 60

Query: 61  SDPKGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADC 120
           +DP+GLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+F++N D 
Sbjct: 61  TDPEGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDS 120

Query: 121 FEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPYHK 180
           F EALDRFAQFFI+PLMS DATMREIKAVDSE+Q NLLSD WRM QLQ+H+S E HPYHK
Sbjct: 121 FYEALDRFAQFFIQPLMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHK 180

Query: 181 FSTGNWDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIFQD 240
           FSTGN DTL V+P+  G+DTR ELI+F++++YS+NIMHLVVY KENLDK Q LVE +FQ 
Sbjct: 181 FSTGNMDTLHVRPEENGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQG 240

Query: 241 IPNHNCNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHL 300
           I N N     FPGQPCT +HL+VLV+A+PI QGH+L + WP+TP I HY+E PCRYLG L
Sbjct: 241 IRNTNQGIPRFPGQPCTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDL 300

Query: 301 IGHEGEGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLFKY 360
           IGHEGEGSL++ LK LGWATGL AGE+D +  +SFF V IDLTDAG EHMQDI+GLLF+Y
Sbjct: 301 IGHEGEGSLFHALKILGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEY 360

Query: 361 IDLLKQSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSLPS 420
           I +L+QSG+ +WIFDELSAICE +FHYQ KI PI Y V++S NM++YP + WLVGSSLPS
Sbjct: 361 IKVLQQSGVSQWIFDELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPS 420

Query: 421 KFNPNLIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWMLSA 480
           KFNP ++  VLD+LS  N RIFWES KFEGQTDKVEPWY T YS+E I+   IQEWM SA
Sbjct: 421 KFNPAIVQKVLDELSPNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSA 480

Query: 481 PDVKLHLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKIDF 540
           PDV L LP PN+FIPTD SLKD  +K  FPVLLRK+SYS LWYKPDT FF PKAYVK+DF
Sbjct: 481 PDVNLLLPTPNVFIPTDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDF 540

Query: 541 RCPHAGISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKL 600
            CP A  SP+A VL+DIF  LL+DYLNEYAY AQ AGL YG++ ++ GF+++L G+NHKL
Sbjct: 541 NCPLAVSSPDAAVLSDIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKL 600

Query: 601 RVLLETVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLM 660
           R+LLE V++KIA+F VKPDRF VIKETV K YQN KFQQP++QA  YCSL+LQD  WP  
Sbjct: 601 RILLEAVIQKIAKFEVKPDRFSVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWT 660

Query: 661 EELAILPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPISR 720
           EEL  L HL AEDL  F P LLS   +ECYIAGN+E++E+ESM++HIED+ F    PI R
Sbjct: 661 EELDALSHLEAEDLANFVPMLLSRTFVECYIAGNVEKDEAESMVKHIEDVLFTDSKPICR 720

Query: 721 PLYPSQHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFALV 780
           PL+PSQ  TNRV +L  G+ +FY  EG N +DENSALVHYIQVH+D+F  N KLQLF L+
Sbjct: 721 PLFPSQFLTNRVTELGTGMKHFYYQEGSNSSDENSALVHYIQVHKDEFSMNSKLQLFELI 780

Query: 781 AKQSAFHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESK 840
           AKQ  FHQLRT+EQLGYIT L   NDSG+ G+QFIIQS+ KGP +ID RVE+ L+  ESK
Sbjct: 781 AKQDTFHQLRTIEQLGYITSLSLSNDSGVYGVQFIIQSSVKGPGHIDSRVESLLKDLESK 840

Query: 841 LVDMTIDEFK 846
             +M+ +EFK
Sbjct: 841 FYNMSDEEFK 850

BLAST of MC05g1086 vs. TAIR 10
Match: AT1G06900.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 467.2 bits (1201), Expect = 3.1e-131
Identity = 301/944 (31.89%), Postives = 485/944 (51.38%), Query Frame = 0

Query: 32   EVLLVSDPDTDKSAASMTVNVGSFSDPKGLEGLAHFLEHMLFYASEKYPLEDSYSKYITE 91
            EV    D  T K+AA+M V++GSF DP   +GLAHFLEHMLF  S ++P E+ Y  Y+++
Sbjct: 95   EVKGKGDHQTKKAAAAMCVSMGSFLDPPEAQGLAHFLEHMLFMGSTEFPDENEYDSYLSK 154

Query: 92   HGGSMNAFTASEQTNYYFEVNADCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKN 151
            HGGS NA+T  E T Y+FEV  +  + AL RF+QFF+ PLM  +A  RE+ AVDSE  + 
Sbjct: 155  HGGSSNAYTEMEHTCYHFEVKREFLQGALKRFSQFFVAPLMKTEAMEREVLAVDSEFNQA 214

Query: 152  LLSDGWRMHQLQRHISSESHPYHKFSTGNWDTLEVQPKAKGLDTRHELIRFHEKNYSSNI 211
            L +D  R+ QLQ + S++ HP+++F+ GN  +L       G+D R  +++ +++ Y   +
Sbjct: 215  LQNDACRLQQLQCYTSAKGHPFNRFAWGNKKSLS-GAMENGVDLRECIVKLYKEYYHGGL 274

Query: 212  MHLVVYAKENLDKVQSLVENIFQDIPNHNCNCAHFPGQPCTSEHLKVLVRAIPIKQGHKL 271
            M LVV   E+LD ++S V  +F D+ N +        +    +  K L R   +K  H L
Sbjct: 275  MKLVVIGGESLDMLESWVVELFGDVKNGSKIRPTLEAEGPIWKGGK-LYRLEAVKDVHIL 334

Query: 272  RIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTLGWATGLSAGESDSTTNFS-- 331
             + W + P    Y + P  YL HL+GHEG GSL+  LK  GWAT LSAG  D   N S  
Sbjct: 335  DLTWTLPPLRSAYVKKPEDYLAHLLGHEGRGSLHSFLKAKGWATSLSAGVGDDGINRSSL 394

Query: 332  --FFKVIIDLTDAGQEHMQDIVGLLFKYIDLLKQSGICKWIFDELSAICETKFHYQDKIR 391
               F + I LTD+G E + DI+G +++Y+ LL+     +WIF EL  I    F + ++  
Sbjct: 395  AYVFGMSIHLTDSGLEKIYDIIGYIYQYLKLLRDVSPQEWIFKELQDIGNMDFRFAEEQP 454

Query: 392  PIDYVVNLSLNMQLYPPEDWLVGSSLPSKFNPNLIGTVLDQLSVENFRIFWESKKFEGQT 451
              DY   LS NM  YP E  + G  +   ++P LI  ++   + +N RI   SK  + + 
Sbjct: 455  ADDYAAELSENMLAYPVEHVIYGDYVYQTWDPKLIEDLMGFFTPQNMRIDVVSKSIKSEE 514

Query: 452  DKVEPWYGTTYSIENISGTLIQEWM-LSAPDVKLHLPAPNIFIPTDLSLKDAHEKVE--- 511
             + EPW+G++Y  E++  +L++ W   S  D  LHLP+ N FIP D S++  +  V+   
Sbjct: 515  FQQEPWFGSSYIEEDVPLSLMESWSNPSEVDNSLHLPSKNQFIPCDFSIRAINSDVDPKS 574

Query: 512  --FPVLLRKSSYSTLWYKPDTMFFTPKA--YVKIDFRCPHAGISPEAEVLTDIFTRLLMD 571
               P  +    +   WYK D  F  P+A  Y +I+ +  +A +  +  +LT+++  LL D
Sbjct: 575  QSPPRCIIDEPFMKFWYKLDETFKVPRANTYFRINLKGAYASV--KNCLLTELYINLLKD 634

Query: 572  YLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNHKLRVLLETVVEKIARFNVKPDRFLVI 631
             LNE  Y A +A L   ++      ++ + G+N K+  LL  ++     F    +RF VI
Sbjct: 635  ELNEIIYQASIAKLETSLSMYGDKLELKVYGFNEKIPALLSKILAIAKSFMPNLERFKVI 694

Query: 632  KETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWPLMEELAILPHLGAEDLVKFAPTLLSS 691
            KE + + ++N    +P   + Y    +L    +   E+L++L  L  +DL  F P L S 
Sbjct: 695  KENMERGFRNTN-MKPLNHSTYLRLQLLCKRIYDSDEKLSVLNDLSLDDLNSFIPELRSQ 754

Query: 692  ACLECYIAGNIERNESESMIEHIEDIFFKGPNPISRPLYPSQHPTNRVVKLARGIGYFYS 751
              +E    GN+  +E+ ++    +D     P P S+  +  Q     +     G      
Sbjct: 755  IFIEALCHGNLSEDEAVNISNIFKDSLTVEPLP-SKCRHGEQ-----ITCFPMGAKLVRD 814

Query: 752  AEGLNPNDENSALVHYIQVHRDDFLQ---NVKLQLFALVAKQSAFHQLRTVEQLGYITFL 811
                N ++ NS +  Y Q+  ++         L LF  + ++  F+QLRT EQLGY+   
Sbjct: 815  VNVKNKSETNSVVELYYQIEPEEAQSTRTKAVLDLFHEIIEEPLFNQLRTKEQLGYVVEC 874

Query: 812  MQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFESKLVDMTIDEFKSNVNALIDMKL 871
              R    + G  F +QS+  GP ++  RV+ F++  E  L  +  + ++   + +I   L
Sbjct: 875  GPRLTYRVHGFCFCVQSSKYGPVHLLGRVDNFIKDIEGLLEQLDDESYEDYRSGMIARLL 934

Query: 872  EKFKNLREESGFYWREISDGTLKFDRRESEVATLRTLAHQELINFFNEHIKVGAPRKKAL 931
            EK  +L  E+   W +I D    FD    E   LR++  +++I+++  + +  +P+ + L
Sbjct: 935  EKDPSLLSETNDLWSQIVDKRYMFDFSHKEAEELRSIQKKDVISWYKTYFRESSPKCRRL 994

Query: 932  SVRVYG-NLHSSEYSSDLSQPLQPDTVKIDDIFSFRRSQPLYGS 960
            +VRV+G + +  E  +D     Q     I D  +F+ +   Y S
Sbjct: 995  AVRVWGCDTNMKETQTD-----QKAVQVIADAVAFKSTSKFYPS 1022