MC05g1024 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g1024
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Werner Syndrome-like exonuclease
Location	MC05: 13843220 .. 13843831 (+)
RNA-Seq Expression	MC05g1024
Synteny	MC05g1024

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACCACCAGCGCCAGCCACCGAATCCCCTCCGATTCCGACAATTTATTCGACGTCACCATAGATTCCGATCATATTCGCACTCTCCTCACATCTTCTCCGGCCATGGTCGATTCCTGGATCTCTGAAACCCTCGCGATTCGGACTCCACCTCTCATCGTTGGTTTGTACATCGAGTGGCGCCTCAACGCCAATCGCTCTCACAGAGACCGGGTCACCACATTGGAACTCTGTTTCAATCGCCGATGCCTCATTTTCCAACTGATTCACGCCTCCGAAATCCCTCGCTCTCTGTTCGATTTCTTGAACAACGAGTCGTTCAGTTTCGTCGGAGTTGGAATCGGCAGGCACGTCGAGAAGCTCTACAGCGATCACGGCTTGAGAGTGAGAAATGCTGTGGATCTCATGAGTTTGGTCGAGAATGTGATGGGGAGAAGAGATCTGAGATGTGCGGGATTGAGGGGCTTGGCGAAAGAGATTTTTGGAAAGATGATTTCTGAGCCGAAGGACGCGACGACGTGTAGGTGGGATAATGAATGGCTGACTATGAATCAGGTGTCGTATGCTTCTGTTGATGTGTTTCTGTCATCTGAGATTGGAAGACGCCTT

mRNA sequence

ATGGCTACCACCAGCGCCAGCCACCGAATCCCCTCCGATTCCGACAATTTATTCGACGTCACCATAGATTCCGATCATATTCGCACTCTCCTCACATCTTCTCCGGCCATGGTCGATTCCTGGATCTCTGAAACCCTCGCGATTCGGACTCCACCTCTCATCGTTGGTTTGTACATCGAGTGGCGCCTCAACGCCAATCGCTCTCACAGAGACCGGGTCACCACATTGGAACTCTGTTTCAATCGCCGATGCCTCATTTTCCAACTGATTCACGCCTCCGAAATCCCTCGCTCTCTGTTCGATTTCTTGAACAACGAGTCGTTCAGTTTCGTCGGAGTTGGAATCGGCAGGCACGTCGAGAAGCTCTACAGCGATCACGGCTTGAGAGTGAGAAATGCTGTGGATCTCATGAGTTTGGTCGAGAATGTGATGGGGAGAAGAGATCTGAGATGTGCGGGATTGAGGGGCTTGGCGAAAGAGATTTTTGGAAAGATGATTTCTGAGCCGAAGGACGCGACGACGTGTAGGTGGGATAATGAATGGCTGACTATGAATCAGGTGTCGTATGCTTCTGTTGATGTGTTTCTGTCATCTGAGATTGGAAGACGCCTT

Coding sequence (CDS)

ATGGCTACCACCAGCGCCAGCCACCGAATCCCCTCCGATTCCGACAATTTATTCGACGTCACCATAGATTCCGATCATATTCGCACTCTCCTCACATCTTCTCCGGCCATGGTCGATTCCTGGATCTCTGAAACCCTCGCGATTCGGACTCCACCTCTCATCGTTGGTTTGTACATCGAGTGGCGCCTCAACGCCAATCGCTCTCACAGAGACCGGGTCACCACATTGGAACTCTGTTTCAATCGCCGATGCCTCATTTTCCAACTGATTCACGCCTCCGAAATCCCTCGCTCTCTGTTCGATTTCTTGAACAACGAGTCGTTCAGTTTCGTCGGAGTTGGAATCGGCAGGCACGTCGAGAAGCTCTACAGCGATCACGGCTTGAGAGTGAGAAATGCTGTGGATCTCATGAGTTTGGTCGAGAATGTGATGGGGAGAAGAGATCTGAGATGTGCGGGATTGAGGGGCTTGGCGAAAGAGATTTTTGGAAAGATGATTTCTGAGCCGAAGGACGCGACGACGTGTAGGTGGGATAATGAATGGCTGACTATGAATCAGGTGTCGTATGCTTCTGTTGATGTGTTTCTGTCATCTGAGATTGGAAGACGCCTT

Protein sequence

MATTSASHRIPSDSDNLFDVTIDSDHIRTLLTSSPAMVDSWISETLAIRTPPLIVGLYIEWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVEKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDNEWLTMNQVSYASVDVFLSSEIGRRL

Homology

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BLAST of MC05g1024 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.6e-11
Identity = 43/155 (27.74%), Postives = 75/155 (48.39%), Query Frame = 0


Query: 54  IVGLYIEWRLNANRSHRDRVTTLELCFN-RRCLIFQLIHASEIPRSLFDFLNNESFSFVG 113
           ++G  IEW     +    +V  +++C + ++C +F +   +  P+ L   L +ES   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 114 VGIGRHVEKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCA---GLRGLAKEIFGKMISEP 173
           VGI     KL SD+ L+++  ++L     + M  + LRC       GL K +F + + + 
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIEL-----SEMANQKLRCKEKWTFNGLIKHLFKEQLYKR 191

Query: 174 KDATTCRWDNEWLTMNQVSYASVDVFLSSEIGRRL 205
           K      WD   LT +Q  YA+ D +    I ++L
Sbjct: 192 KSYRCSNWDIFLLTEDQKLYAATDAYAGLLIYKKL 221

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BLAST of MC05g1024 vs. NCBI nr
Match: XP_022147750.1 (Werner Syndrome-like exonuclease [Momordica charantia])

HSP 1 Score: 295 bits (754), Expect = 1.55e-98
Identity = 148/204 (72.55%), Postives = 163/204 (79.90%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDHIRTLLTSSPAMVDSWISETLAIRTPPLIVGLYIE 60
           MA +   H IPSDS NL+DVTIDSD I TLLTSSPAMVDSWISETLAI+TPPLIVGL IE
Sbjct: 1   MAISIVDHEIPSDSHNLYDVTIDSDQIHTLLTSSPAMVDSWISETLAIQTPPLIVGLDIE 60

Query: 61  WRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVE 120
           WR N N S+ + V TL+LCF+RRCLIFQLIHASEIPRSL DFL +ESF+FVGVGIG  VE
Sbjct: 61  WRPNRNSSYENPVATLQLCFDRRCLIFQLIHASEIPRSLTDFLTDESFTFVGVGIGEDVE 120

Query: 121 KLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDNE 180
           KL  D+GL V N VDL +L EN MGR DL+ AGL+GLAKE+ GK IS+PK  T  RWDNE
Sbjct: 121 KLLCDYGLNVSNTVDLRNLAENAMGRGDLKNAGLKGLAKEVLGKEISKPKSVTMSRWDNE 180

Query: 181 WLTMNQVSYASVDVFLSSEIGRRL 204
           WL  NQV YA VD FLSSEIGR L
Sbjct: 181 WLVPNQVKYACVDAFLSSEIGRHL 204

[Close]

BLAST of MC05g1024 vs. NCBI nr
Match: XP_011652174.1 (Werner Syndrome-like exonuclease [Cucumis sativus] >KGN59430.1 hypothetical protein Csa_001395 [Cucumis sativus])

HSP 1 Score: 246 bits (628), Expect = 1.73e-79
Identity = 123/205 (60.00%), Postives = 150/205 (73.17%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           MA T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TLAI+TPPL+VGL I
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+ GK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 204
           EWLT+NQV YA +D F S EIG+ L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGKFL 204

[Close]

BLAST of MC05g1024 vs. NCBI nr
Match: XP_008443244.1 (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo] >ADN33882.1 3'-5' exonuclease [Cucumis melo subsp. melo] >KAA0053896.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa] >TYK25510.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 244 bits (624), Expect = 7.01e-79
Identity = 122/205 (59.51%), Postives = 149/205 (72.68%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           M+ T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TL I+TPPL+VGL I
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+ GK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 204
           EWLT+NQV YA +D F S EIGR L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGRFL 204

[Close]

BLAST of MC05g1024 vs. NCBI nr
Match: XP_038903327.1 (Werner Syndrome-like exonuclease isoform X2 [Benincasa hispida])

HSP 1 Score: 233 bits (594), Expect = 1.22e-74
Identity = 116/188 (61.70%), Postives = 143/188 (76.06%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           MA T   H++PSDS N FD+T+DSD  I TLLT+SP+MV++WISETLA++TPPL+VGL I
Sbjct: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTNSPSMVENWISETLALQTPPLMVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QLIH  EIP+SLF+FL NES +FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCMGRRCLILQLIHTPEIPKSLFEFLENESLTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   VDL +L E+V GRRDL+ AGL+ L KE+ GK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRVDLRNLAESVTGRRDLKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQV 187
           EWLT+NQ+
Sbjct: 181 EWLTLNQM 187

[Close]

BLAST of MC05g1024 vs. NCBI nr
Match: KAG6580879.1 (Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017635.1 Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 234 bits (596), Expect = 1.26e-74
Identity = 124/205 (60.49%), Postives = 144/205 (70.24%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           MA T      PS S N FDVT +SD  I TLLT SP MVD WISETL  +TPPLIVGL I
Sbjct: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTPPLIVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N  RS+ + V TL+LC  RRCLI QLI+A E P+SL++FL NES SFVGVGI    
Sbjct: 61  EWRPNI-RSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDN 180
           EKL  D+GL+VR  VDL +L E+V GRR+L+ AGL+ LAKEI GK I +P+  T  RWD 
Sbjct: 121 EKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 204
           EWLT NQ+ YA  D F+S EIGR L
Sbjct: 181 EWLTHNQIKYACFDAFVSYEIGRHL 204

[Close]

BLAST of MC05g1024 vs. ExPASy TrEMBL
Match: A0A6J1D107 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016613 PE=4 SV=1)

HSP 1 Score: 295 bits (754), Expect = 7.51e-99
Identity = 148/204 (72.55%), Postives = 163/204 (79.90%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDHIRTLLTSSPAMVDSWISETLAIRTPPLIVGLYIE 60
           MA +   H IPSDS NL+DVTIDSD I TLLTSSPAMVDSWISETLAI+TPPLIVGL IE
Sbjct: 1   MAISIVDHEIPSDSHNLYDVTIDSDQIHTLLTSSPAMVDSWISETLAIQTPPLIVGLDIE 60

Query: 61  WRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVE 120
           WR N N S+ + V TL+LCF+RRCLIFQLIHASEIPRSL DFL +ESF+FVGVGIG  VE
Sbjct: 61  WRPNRNSSYENPVATLQLCFDRRCLIFQLIHASEIPRSLTDFLTDESFTFVGVGIGEDVE 120

Query: 121 KLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDNE 180
           KL  D+GL V N VDL +L EN MGR DL+ AGL+GLAKE+ GK IS+PK  T  RWDNE
Sbjct: 121 KLLCDYGLNVSNTVDLRNLAENAMGRGDLKNAGLKGLAKEVLGKEISKPKSVTMSRWDNE 180

Query: 181 WLTMNQVSYASVDVFLSSEIGRRL 204
           WL  NQV YA VD FLSSEIGR L
Sbjct: 181 WLVPNQVKYACVDAFLSSEIGRHL 204

[Close]

BLAST of MC05g1024 vs. ExPASy TrEMBL
Match: A0A0A0LBN3 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819870 PE=4 SV=1)

HSP 1 Score: 246 bits (628), Expect = 8.37e-80
Identity = 123/205 (60.00%), Postives = 150/205 (73.17%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           MA T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TLAI+TPPL+VGL I
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+ GK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 204
           EWLT+NQV YA +D F S EIG+ L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGKFL 204

[Close]

BLAST of MC05g1024 vs. ExPASy TrEMBL
Match: A0A5A7UI20 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006600 PE=4 SV=1)

HSP 1 Score: 244 bits (624), Expect = 3.39e-79
Identity = 122/205 (59.51%), Postives = 149/205 (72.68%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           M+ T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TL I+TPPL+VGL I
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+ GK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 204
           EWLT+NQV YA +D F S EIGR L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGRFL 204

[Close]

BLAST of MC05g1024 vs. ExPASy TrEMBL
Match: E5GBP0 (3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 244 bits (624), Expect = 3.39e-79
Identity = 122/205 (59.51%), Postives = 149/205 (72.68%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           M+ T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TL I+TPPL+VGL I
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+ GK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 204
           EWLT+NQV YA +D F S EIGR L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGRFL 204

[Close]

BLAST of MC05g1024 vs. ExPASy TrEMBL
Match: A0A1S3B738 (Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV=1)

HSP 1 Score: 244 bits (624), Expect = 3.39e-79
Identity = 122/205 (59.51%), Postives = 149/205 (72.68%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           M+ T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TL I+TPPL+VGL I
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+ GK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 204
           EWLT+NQV YA +D F S EIGR L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGRFL 204

[Close]

BLAST of MC05g1024 vs. TAIR 10
Match: AT3G12430.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 3.6e-13
Identity = 50/196 (25.51%), Postives = 88/196 (44.90%), Query Frame = 0


Query: 30  LLTSSPAMVDSWISETLA---IRTPPLIVGLYIEWRLNA------------------NRS 89
           ++T  P+++  WI + L+     + PL+VG+ ++W  ++                   RS
Sbjct: 29  IVTPDPSVIGQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRYRSDSPPVRYRS 88

Query: 90  HRDRV--------TTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVE 149
              RV         TL+LC   RC+I QL H   +P+ L +FL +  ++FVG+   +   
Sbjct: 89  DSPRVRRRFDPPADTLQLCVGNRCIIIQLSHCERVPQVLRNFLADRDYTFVGIWNSQDAG 148

Query: 150 KL-YSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCRWDN 196
           KL  S H L +   +DL   V +  GR   RC+  + + + +    +   +  +   W  
Sbjct: 149 KLKRSKHELEIAVLLDLRKFVSDSSGRTMKRCSFEKIVEENLGHSGVRLDRKVSRSDWRV 208

[Close]

BLAST of MC05g1024 vs. TAIR 10
Match: AT3G12460.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 8.9e-12
Identity = 48/209 (22.97%), Postives = 89/209 (42.58%), Query Frame = 0


Query: 18  FDVTIDSDHIRTLLTSSPAMVDSWISETL---AIRTPPLIVGLYIEWRLNANRSHRDRVT 77
           + V    + +   +T  P+++  WI +      + + PL+VG+ ++W  +        V+
Sbjct: 17  YSVDFFGEELFVTVTPDPSVIGQWIHDVFFHNRLSSHPLVVGVGVQWTPSGYHPASPPVS 76

Query: 78  --------------------------TLELCFNRRCLIFQLIHASEIPRSLFDFLNNESF 137
                                     TL+LC   RC+I QL +   +P+ L +FL +   
Sbjct: 77  YRSDSSPDSYRSDSPPVFYSSDPPADTLQLCVGNRCIIIQLRYCERVPQVLRNFLADRDN 136

Query: 138 SFVGVGIGRHVEKL-YSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEIFG-KMI 196
           +FVG+   +   KL  S H L +   +DL   V +  GRR +    L  + +E  G   +
Sbjct: 137 TFVGIWNSQDAGKLERSRHQLEIAELMDLREFVSDSSGRRSMYNYSLEKIVEENLGYPGV 196

[Close]

BLAST of MC05g1024 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-11
Identity = 46/156 (29.49%), Postives = 72/156 (46.15%), Query Frame = 0


Query: 52  PLIVGLYIEWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFV 111
           PL+VGL ++W    +    D    L+LC   RCLI QL H   IP  L  FL +E+ +FV
Sbjct: 55  PLVVGLDVQWTPGGSDPPPD---ILQLCVGNRCLIIQLSHCKRIPEVLRSFLEDETITFV 114

Query: 112 GVGIGRHVEKLYS-DHGLRVRNAVDL-----MSLVENVMGRRDLRCAGLRGLAKEIFGKM 171
           GV   +   KL    H L +   +D+       L+ +   +    C G +G+ K+     
Sbjct: 115 GVWNSQDQGKLERFRHQLEIWRLLDIRHYLPTRLLNSSFEKIVEECLGYKGVRKD----- 174

Query: 172 ISEPKDATTCRWDNEWLTMNQVSYASVDVFLSSEIG 202
               K+     W    L+ +Q+  AS DV++  ++G
Sbjct: 175 ----KEICMSNWGARSLSHDQIVQASDDVYVCCKLG 198

[Close]

BLAST of MC05g1024 vs. TAIR 10
Match: AT3G12470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 4.9e-10
Identity = 48/179 (26.82%), Postives = 83/179 (46.37%), Query Frame = 0


Query: 25  DHIRTLLTSSPAMVDSWISETLAIR----TPPLIVGLYIEWRLNANRSHRDRVTTLELCF 84
           D +   +T + +++  WI    +        PL+VG+ ++WR +++        TL+LC 
Sbjct: 24  DDLIVTVTPTASVIRRWIRSVRSYNRNHSVHPLVVGIGVQWRPDSS-DPDPPPKTLQLCV 83

Query: 85  NRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVEKLYS-DHGLRVRNAVDLMSL 144
             RCLI QL +   +P+ L  FL +   +FVGV  G+  +KL    H + +   +D+   
Sbjct: 84  GSRCLIIQLGYNYGLPKVLRTFLADPKTTFVGVWNGQDQKKLEKCRHRVEIGKLLDIRMF 143

Query: 145 VENVMGRRDLRCAGLRGLAKEIFGKMISEPKDATTCR--WDNEWLTMNQVSYASVDVFL 197
           V +  G R   C+    + KE  G+ +    D   C   W    L   QV  AS++ ++
Sbjct: 144 VRDSRGARMCFCS-FEQIVKERLGR-VGVRLDPAICMSDWGVYNLNHYQVLQASIESYV 199

[Close]

BLAST of MC05g1024 vs. TAIR 10
Match: AT3G12440.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 8.4e-10
Identity = 41/136 (30.15%), Postives = 67/136 (49.26%), Query Frame = 0


Query: 75  TLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVEKL-YSDHGLRVRNA 134
           TL+LC   RC+I QL + + +P  L  FL + + +FVG    +   KL  S H L +   
Sbjct: 218 TLQLCVGNRCIIIQLFYCNRVPHVLRRFLGDRNHTFVGFWNSQDAGKLKRSRHRLEIAEL 277

Query: 135 VDLMSLVENVMGRRDLRCAGLRGLAKEIF------GKMISEPKDATTCRWDNEWLTMNQV 194
           +DL + V +  GR      GL+G + E+        + +   K      W    L+ +Q+
Sbjct: 278 LDLRNHVVDSRGR------GLKGRSFEVVVGECLGYRGVRLEKAIGMSDWSVYDLSYDQI 337

Query: 195 SYASVDVFLSSEIGRR 204
             AS+DV++ S++G R
Sbjct: 338 LQASIDVYVCSKLGFR 347

The following BLAST results are available for this feature:

BLAST of MC05g1024 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O93530	5.6e-11	27.74	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]

BLAST of MC05g1024 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147750.1	1.55e-98	72.55	Werner Syndrome-like exonuclease [Momordica charantia]	[more]
XP_011652174.1	1.73e-79	60.00	Werner Syndrome-like exonuclease [Cucumis sativus] >KGN59430.1 hypothetical prot...	[more]
XP_008443244.1	7.01e-79	59.51	PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo] >ADN33882.1 3'-5' exo...	[more]
XP_038903327.1	1.22e-74	61.70	Werner Syndrome-like exonuclease isoform X2 [Benincasa hispida]	[more]
KAG6580879.1	1.26e-74	60.49	Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of MC05g1024 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D107	7.51e-99	72.55	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016613 ...	[more]
A0A0A0LBN3	8.37e-80	60.00	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A5A7UI20	3.39e-79	59.51	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
E5GBP0	3.39e-79	59.51	3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A1S3B738	3.39e-79	59.51	Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV...	[more]

BLAST of MC05g1024 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12430.1	3.6e-13	25.51	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12460.1	8.9e-12	22.97	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G36110.1	1.2e-11	29.49	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12470.1	4.9e-10	26.82	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12440.1	8.4e-10	30.15	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 14..204 e-value: 1.4E-44 score: 154.0
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 40..196 e-value: 4.2E-10 score: 39.7
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 10..204
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 10..204
None	No IPR available	CDD	cd06141	WRN_exo	coord: 32..204 e-value: 1.45976E-46 score: 149.266
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 46..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g1024.1	MC05g1024.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding