MC05g0971 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0971
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	MC05: 10637450 .. 10639116 (+)
RNA-Seq Expression	MC05g0971
Synteny	MC05g0971

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGGGCGAGAGATGTTCTTCAGGGTGATAAGGATGGGGTTCGAAGACATATACGTATCAAGTCGAAGTTCATCATCCAACAAGTATGGCAAAGACTACTTCATATGCACTAGCCCTGCTTCCACGTTGGTTCCTGTGACTATAAAACCTGGTGAAGAGGGGAGGGGCGCCTAAGTGATCGAGCATGATGACTTGTAATTAATTTTCTCTCTTCAAAATCCACATTTTACCCAAATTCATTGTGAACATTACACCACATGATCAAGTAAAGTAGCAGCCAAAATTTACATATGCTCCTCCATTTTCGAGGACTTTATTAATGAGTTTCTCTGGTCTCCTGAATGTGATTATACTATTCACAAGCTGGCACTGTTCTATTATAATATAGATCAATGAGCAGAATAACTACTTTTTTTATTACAGGATTTATCAATCTCAATCTCCTCTGTTTGCCTACAATGGCTTCACCTGAGGGAAGAATAGTCCCCGCAGCGGTTGCTCTGCCGCTGCAGGATCGAGTGGCTATCGTGACTGGCGCCTCTCGTGGCATTGGCCGAGGCGTCGCCCTCCACCTTGCTGCTCTTGGCGCCAGAGTTGTAGTAAACTACCTATCCAGTTCGGCTGCAGCCAGTCGTGTTGCTGCCGAGATCAACTCCTCCCCTGCAGCCGACATCGCGGGGAGCTACCCCCGAGCCGTTACGTGTCAGGCAGATGTGTCTAAGCCAGACGAAGTGAAGGCCCTATTCGACACTGCCGAGGAGGCATTCGGGTCCCCAGTCCATATCCTGGTCAACTCTGCCGGAATTTGGGATCCTACATATCCATATATTGCAGATACATCTTTGGAGATTTTCGACCGAATTTTCAGGTCTTCTCTATTCTAGAATACTATTTTGTACTTTCAAATGTCAAAATTTTGCTTTTTACTCTCAATTAGTGAAGCTTAAATTGAGGTGGGTGAGTATATTTTTAGTGTGAACGCAATGGGGAGTTTCCTGTGCTGTAAAGAAGCGGCAAAGAGAGTGAAAGGCGACGGTGGGGGGCGGATTATAATGATCTCGTCGTCAGCGGTGGCGGCACCGTCGCCGGGAATAGGGGCGTACACGGCGTCGAAGGCGGCGGTGGAGGCGATGACGAAGACTCTGGCAAAGGAACTGAGAGGGACGGGGATTTCGGCGAACTGCGTAGCGCCGGGAGCGACGGCGACAGACATGTTGTACGGAGGGATGGATGAGGAAGCAGTGAAGAAGGTGATAGAAAAGTGTCCGTTGGGGCGGCTTGGGTATCCGACGGACGTGGCTTATTTGGTGGGCTTTTTGGCTTCCGACGACGGTGAGTGGATCAATGGCCAGCTCTTGCTCGTCAATGGCGGCATCGTCTAGTTTCTTTGAGATTTGTCCGATGTGTTGTGCTGTGCTGTATTGTTTTGTGTTTTGGATTTGGAATCTTGGACAATTGTTTTGAGCAACTCTCACTCAACTAAAAAACGAAAGAAGGATAATTTAATTTGAGCGTTTAAATATTTCCTTGCCTTTGTAACCATCTCTTTATTTATTTATTATTATTATTATTTTATGAGTAAATTTTGATTGGATAACTTTATTTTTCTAAAAATGGGTTTTTGAATAGTCAAATGCAATTAATAATATACTCTAATCTTATTCCGT

mRNA sequence

CAGGGGCGAGAGATGTTCTTCAGGGTGATAAGGATGGGGTTCGAAGACATATACGTATCAAGTCGAAGTTCATCATCCAACAAGTATGGCAAAGACTACTTCATATGCACTAGCCCTGCTTCCACGTTGGTTCCTGTGACTATAAAACCTGGTGAAGAGGGGAGGGGCGCCTTTATCAATCTCAATCTCCTCTGTTTGCCTACAATGGCTTCACCTGAGGGAAGAATAGTCCCCGCAGCGGTTGCTCTGCCGCTGCAGGATCGAGTGGCTATCGTGACTGGCGCCTCTCGTGGCATTGGCCGAGGCGTCGCCCTCCACCTTGCTGCTCTTGGCGCCAGAGTTGTAGTAAACTACCTATCCAGTTCGGCTGCAGCCAGTCGTGTTGCTGCCGAGATCAACTCCTCCCCTGCAGCCGACATCGCGGGGAGCTACCCCCGAGCCGTTACGTGTCAGGCAGATGTGTCTAAGCCAGACGAAGTGAAGGCCCTATTCGACACTGCCGAGGAGGCATTCGGGTCCCCAGTCCATATCCTGGTCAACTCTGCCGGAATTTGGGATCCTACATATCCATATATTGCAGATACATCTTTGGAGATTTTCGACCGAATTTTCAGTGTGAACGCAATGGGGAGTTTCCTGTGCTGTAAAGAAGCGGCAAAGAGAGTGAAAGGCGACGGTGGGGGGCGGATTATAATGATCTCGTCGTCAGCGGTGGCGGCACCGTCGCCGGGAATAGGGGCGTACACGGCGTCGAAGGCGGCGGTGGAGGCGATGACGAAGACTCTGGCAAAGGAACTGAGAGGGACGGGGATTTCGGCGAACTGCGTAGCGCCGGGAGCGACGGCGACAGACATGTTGTACGGAGGGATGGATGAGGAAGCAGTGAAGAAGGTGATAGAAAAGTGTCCGTTGGGGCGGCTTGGGTATCCGACGGACGTGGCTTATTTGGTGGGCTTTTTGGCTTCCGACGACGGTGAGTGGATCAATGGCCAGCTCTTGCTCGTCAATGGCGGCATCGTCTAGTTTCTTTGAGATTTGTCCGATGTGTTGTGCTGTGCTGTATTGTTTTGTGTTTTGGATTTGGAATCTTGGACAATTGTTTTGAGCAACTCTCACTCAACTAAAAAACGAAAGAAGGATAATTTAATTTGAGCGTTTAAATATTTCCTTGCCTTTGTAACCATCTCTTTATTTATTTATTATTATTATTATTTTATGAGTAAATTTTGATTGGATAACTTTATTTTTCTAAAAATGGGTTTTTGAATAGTCAAATGCAATTAATAATATACTCTAATCTTATTCCGT

Coding sequence (CDS)

CAGGGGCGAGAGATGTTCTTCAGGGTGATAAGGATGGGGTTCGAAGACATATACGTATCAAGTCGAAGTTCATCATCCAACAAGTATGGCAAAGACTACTTCATATGCACTAGCCCTGCTTCCACGTTGGTTCCTGTGACTATAAAACCTGGTGAAGAGGGGAGGGGCGCCTTTATCAATCTCAATCTCCTCTGTTTGCCTACAATGGCTTCACCTGAGGGAAGAATAGTCCCCGCAGCGGTTGCTCTGCCGCTGCAGGATCGAGTGGCTATCGTGACTGGCGCCTCTCGTGGCATTGGCCGAGGCGTCGCCCTCCACCTTGCTGCTCTTGGCGCCAGAGTTGTAGTAAACTACCTATCCAGTTCGGCTGCAGCCAGTCGTGTTGCTGCCGAGATCAACTCCTCCCCTGCAGCCGACATCGCGGGGAGCTACCCCCGAGCCGTTACGTGTCAGGCAGATGTGTCTAAGCCAGACGAAGTGAAGGCCCTATTCGACACTGCCGAGGAGGCATTCGGGTCCCCAGTCCATATCCTGGTCAACTCTGCCGGAATTTGGGATCCTACATATCCATATATTGCAGATACATCTTTGGAGATTTTCGACCGAATTTTCAGTGTGAACGCAATGGGGAGTTTCCTGTGCTGTAAAGAAGCGGCAAAGAGAGTGAAAGGCGACGGTGGGGGGCGGATTATAATGATCTCGTCGTCAGCGGTGGCGGCACCGTCGCCGGGAATAGGGGCGTACACGGCGTCGAAGGCGGCGGTGGAGGCGATGACGAAGACTCTGGCAAAGGAACTGAGAGGGACGGGGATTTCGGCGAACTGCGTAGCGCCGGGAGCGACGGCGACAGACATGTTGTACGGAGGGATGGATGAGGAAGCAGTGAAGAAGGTGATAGAAAAGTGTCCGTTGGGGCGGCTTGGGTATCCGACGGACGTGGCTTATTTGGTGGGCTTTTTGGCTTCCGACGACGGTGAGTGGATCAATGGCCAGCTCTTGCTCGTCAATGGCGGCATCGTCTAG

Protein sequence

QGREMFFRVIRMGFEDIYVSSRSSSSNKYGKDYFICTSPASTLVPVTIKPGEEGRGAFINLNLLCLPTMASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV

Homology

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BLAST of MC05g0971 vs. ExPASy Swiss-Prot
Match: Q9SQR4 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g03980 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.2e-82
Identity = 161/259 (62.16%), Postives = 194/259 (74.90%), Query Frame = 0


Query: 83  LPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPA-ADIA 142
           LPL  RVAIVTG+SRGIGR +A+HLA LGAR+V+NY S +A A RVA+EIN  P   +I 
Sbjct: 12  LPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERVASEINDFPVREEIT 71

Query: 143 GSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFD 202
           G  PRA+  QA+VS+P +VK++FD AE AF +PVHILVNSAGI DP YP IADTS+E FD
Sbjct: 72  GKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDPKYPTIADTSVEDFD 131

Query: 203 RIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKT 262
             FSVN  G+FLC KEAA R+K  GGGRII+++SS   +  PG GAY ASKAAVE M K 
Sbjct: 132 HTFSVNTKGAFLCSKEAANRLKQGGGGRIILLTSSQTRSLKPGFGAYAASKAAVETMVKI 191

Query: 263 LAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLA 322
           LAKEL+GTGI+ANCVAPG  AT+M + G   E V+K+  + P GR+G   DV  LVGFLA
Sbjct: 192 LAKELKGTGITANCVAPGPIATEMFFDGKTPELVEKIAAESPFGRVGEAKDVVPLVGFLA 251

Query: 323 SDDGEWINGQLLLVNGGIV 341
            D GEW+NGQ++ VNGG V
Sbjct: 252 GDGGEWVNGQIIPVNGGYV 270

[Close]

BLAST of MC05g0971 vs. ExPASy Swiss-Prot
Match: Q9SQR2 (NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ChlADR2 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 9.2e-75
Identity = 150/258 (58.14%), Postives = 189/258 (73.26%), Query Frame = 0


Query: 83  LPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEI--NSSPAADI 142
           L L  RVAIVTG+SRGIGR +A+HLA LGARVVVNY +S   A +VA  I  N S  A++
Sbjct: 12  LCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATAITTNCSKDAEV 71

Query: 143 AGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIF 202
           AG  PR +  +AD+S+P +VK+LFD AE  F SPVHILVNSA I DP +  I+D S+E+F
Sbjct: 72  AGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNHSTISDMSVELF 131

Query: 203 DRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTK 262
           DRI SVN  G+F+C +EAA R+K  GGGRII++S+S V   +   G+YTASKAAVEAM K
Sbjct: 132 DRIISVNTRGAFICAREAANRLKRGGGGRIILLSTSLVQTLNTNYGSYTASKAAVEAMAK 191

Query: 263 TLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFL 322
            LAKEL+GT I+ NCV+PG  AT+M Y G+  E V+KV  +   GR+G   D+A +VGFL
Sbjct: 192 ILAKELKGTEITVNCVSPGPVATEMFYTGLSNEIVEKVKSQNLFGRIGETKDIAPVVGFL 251

Query: 323 ASDDGEWINGQLLLVNGG 339
           ASD GEWINGQ+++ NGG
Sbjct: 252 ASDAGEWINGQVIMANGG 269

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BLAST of MC05g0971 vs. ExPASy Swiss-Prot
Match: Q08632 (Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-72
Identity = 154/258 (59.69%), Postives = 189/258 (73.26%), Query Frame = 0


Query: 83  LPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPAADIAG 142
           LPL  RVAIVTGASRGIGR +AL++A  GA+VV++Y S+  AA  VA+ IN+   +  +G
Sbjct: 17  LPLGGRVAIVTGASRGIGREIALNMAEKGAKVVIHYSSNQHAAEEVASIINNKSPS--SG 76

Query: 143 SYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDR 202
              RA+ C+ADV++P +V  LFDTAE AFG P+HI+VN+AG+ D  YP +A TS E +DR
Sbjct: 77  DGVRAIVCKADVAEPSQVAQLFDTAEHAFG-PLHIVVNNAGVTDSKYPTLAQTSDEEWDR 136

Query: 203 IFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKTL 262
           IF VN  G+FLC +EAAKRV   GGGRII ISSS VA P P  GAYTASKAAVE MT+ L
Sbjct: 137 IFQVNCKGAFLCSREAAKRVVRGGGGRIINISSSLVAMPIPRYGAYTASKAAVEMMTRIL 196

Query: 263 AKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLAS 322
           A+ELRGT I+ANCVAPG  ATDM + G  E AV+  ++  P  RLG   DVA LV FLAS
Sbjct: 197 AQELRGTQITANCVAPGPVATDMFFAGKSEAAVEAGVKSNPFERLGKVEDVAPLVAFLAS 256

Query: 323 DDGEWINGQLLLVNGGIV 341
           D+GEW+N Q++ VNGG V
Sbjct: 257 DEGEWVNAQVVRVNGGQV 271

[Close]

BLAST of MC05g0971 vs. ExPASy Swiss-Prot
Match: W3XC32 (Trihydroxynaphthalene reductase PfmaI OS=Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) OX=1229662 GN=Pfmaj PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 7.6e-37
Identity = 103/265 (38.87%), Postives = 157/265 (59.25%), Query Frame = 0


Query: 85  LQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPAADIAGSY 144
           L+ +VA+VTGA RGIGR +A+ L   GA+V+VNY +SS +A  V   I  S      GS 
Sbjct: 27  LEGKVALVTGAGRGIGREMAMELGRRGAKVIVNYANSSESAQEVVNAIKKS------GS- 86

Query: 145 PRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDRIF 204
             AV  +A+VS  D++ +LFD A + +G  +HI+ +++G+   ++ ++ D + E FDR+F
Sbjct: 87  -DAVAIKANVSDVDQIVSLFDQAVKVWGK-LHIVCSNSGV--VSFGHVKDVTPEEFDRVF 146

Query: 205 SVNAMGSFLCCKEAAKRVKGDGGGRIIMISS-SAVAAPSPGIGAYTASKAAVEAMTKTLA 264
           ++N  G F   +EA K +  + GGR+I++ S +  A   P    Y+ SK A+E   + +A
Sbjct: 147 TINTRGQFFVAREAYKHL--EVGGRLILMGSITGQAKGVPKHAVYSGSKGAIETFVRCMA 206

Query: 265 KELRGTGISANCVAPGATATDMLY--------GG--MDEEAVKKVIEK-CPLGRLGYPTD 324
            +     I+ N VAPG   TDM +        GG  +D+EAV +      PLGR+G P D
Sbjct: 207 VDFGDKKITVNAVAPGGIKTDMYHAVCREYIPGGTELDDEAVDEYASTWSPLGRVGLPID 266

Query: 325 VAYLVGFLASDDGEWINGQLLLVNG 338
           +A  V FLAS DGEW+NG++L ++G
Sbjct: 267 IARCVCFLASQDGEWVNGKVLGIDG 278

[Close]

BLAST of MC05g0971 vs. ExPASy Swiss-Prot
Match: A0A411PQN6 (Short chain dehydrogenase AgnL6 OS=Paecilomyces divaricatus OX=644132 GN=AgnL6 PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.2e-36
Identity = 106/273 (38.83%), Postives = 158/273 (57.88%), Query Frame = 0


Query: 78  PAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPA 137
           P  +   L  +VA+VTGA RGIG  +A+ LA  GA+V+VNY  SS+ A+ V  EI S   
Sbjct: 7   PPHLPYRLDGKVALVTGAGRGIGAAMAVELARCGAKVIVNYARSSSFANAVVDEIKS--- 66

Query: 138 ADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSL 197
               GS   AV  QADVS+  +   LF+ A + FG  + I+ ++AG+   ++ ++++ + 
Sbjct: 67  ---LGS--DAVAIQADVSQVSQTVRLFEEAVQVFGG-LDIVCSNAGV--VSFGHLSEVTE 126

Query: 198 EIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISS-SAVAAPSPGIGAYTASKAAVE 257
           E FDR+FSVN  G F   +EA + +  +  GRII++SS +A +   P    Y+ASK A+ 
Sbjct: 127 EEFDRVFSVNTRGQFFVAREAYRHL--NSPGRIILMSSNTADSFTVPKHSLYSASKGAIN 186

Query: 258 AMTKTLAKELRGTGISANCVAPGATATDMLY----------GGMDEEAVKKVIEK-CPLG 317
              + LAK+     I+ N VAPG T TDM +            M +E + +++    PL 
Sbjct: 187 TFVRCLAKDCGDKKITVNAVAPGGTVTDMFHETAKDYLPNADKMSKEQILQIVANVSPLT 246

Query: 318 RLGYPTDVAYLVGFLASDDGEWINGQLLLVNGG 339
           R GYP D+A +V FLAS + EW+NG++L V+GG
Sbjct: 247 RCGYPIDIAKVVCFLASSESEWVNGKVLGVDGG 266

[Close]

BLAST of MC05g0971 vs. NCBI nr
Match: XP_022155881.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 544 bits (1401), Expect = 1.53e-193
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 58  FINLNLLCLPTMASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVN 117
           FINLNLLCLPTMASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVN
Sbjct: 12  FINLNLLCLPTMASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVN 71

Query: 118 YLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHI 177
           YLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHI
Sbjct: 72  YLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHI 131

Query: 178 LVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSA 237
           LVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSA
Sbjct: 132 LVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSA 191

Query: 238 VAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKK 297
           VAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKK
Sbjct: 192 VAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKK 251

Query: 298 VIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 340
           VIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV
Sbjct: 252 VIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 294

[Close]

BLAST of MC05g0971 vs. NCBI nr
Match: XP_022155882.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 520 bits (1339), Expect = 1.90e-184
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0


Query: 69  MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 128
           MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV
Sbjct: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60

Query: 129 AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 188
           AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT
Sbjct: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120

Query: 189 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 248
           YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY
Sbjct: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180

Query: 249 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 308
           TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG
Sbjct: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240

Query: 309 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 340
           YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV
Sbjct: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 272

[Close]

BLAST of MC05g0971 vs. NCBI nr
Match: KGN59874.2 (hypothetical protein Csa_001420 [Cucumis sativus])

HSP 1 Score: 467 bits (1202), Expect = 2.87e-158
Identity = 252/354 (71.19%), Postives = 282/354 (79.66%), Query Frame = 0


Query: 1   QGREMFFRVIRMGFEDIYVSSRSSSSNKYGKDYFICTSPASTLVPVTIKPGEEGRGA--- 60
           QGRE+FFRVIRMGFEDIYVSS  S SNK+GKD+FICT PAS LVPVT+KPGE+ RGA   
Sbjct: 284 QGRELFFRVIRMGFEDIYVSSPGSLSNKFGKDHFICTGPASMLVPVTVKPGEQWRGAQYR 343

Query: 61  -----------FINLNLLCLPTMASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALH 120
                       I  +   + TMAS  G   PA  ALPLQDRVAIVTGASRGIGRG+ALH
Sbjct: 344 SISSAAAFLSTIIYQSQPLVSTMASESG---PAVPALPLQDRVAIVTGASRGIGRGIALH 403

Query: 121 LAALGARVVVNYLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDT 180
           LA LGARVVVNY+SSSA A +V A+INSS AA   GS  RA+  +ADVS P++VK+LFD 
Sbjct: 404 LAGLGARVVVNYVSSSAEADQVVADINSSSAA---GSSQRAIAWRADVSDPEQVKSLFDA 463

Query: 181 AEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDG 240
           AE+AFGS VHILVNSAGI DPTYPYIA+T LEIFD +FSVN  G FLCCKEAA RVK  G
Sbjct: 464 AEQAFGSQVHILVNSAGISDPTYPYIANTPLEIFDHLFSVNTRGCFLCCKEAANRVKRGG 523

Query: 241 GGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDML 300
           GGRII+ISS+AVAA + G+GAYTASKAAVEAM K  AKEL GTGIS NC+APGATAT+M 
Sbjct: 524 GGRIILISSTAVAATTAGLGAYTASKAAVEAMAKVTAKELSGTGISVNCIAPGATATEMF 583

Query: 301 YGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 340
           Y G+DEE VKKVI+KCP+GRLG P DVA  VGFLASDDGEWINGQ++LVNGGIV
Sbjct: 584 YKGIDEEGVKKVIDKCPMGRLGVPKDVASFVGFLASDDGEWINGQVILVNGGIV 631

[Close]

BLAST of MC05g0971 vs. NCBI nr
Match: KAA0051280.1 (photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa] >TYK29981.1 photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa])

HSP 1 Score: 461 bits (1186), Expect = 7.28e-156
Identity = 248/354 (70.06%), Postives = 281/354 (79.38%), Query Frame = 0


Query: 1   QGREMFFRVIRMGFEDIYVSSRSSSSNKYGKDYFICTSPASTLVPVTIKPGEEGRGA--- 60
           QGRE+FFRVIRMGFEDIYVSS  S SNK+GKD+FICT PAS LVPVT+KPGE+ +GA   
Sbjct: 283 QGRELFFRVIRMGFEDIYVSSPGSLSNKFGKDHFICTGPASMLVPVTVKPGEQWKGAQYR 342

Query: 61  -------FINLNLL----CLPTMASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALH 120
                  F+   +      + TMAS  G   PA  ALPLQDRVAIVTGASRGIGRG+ALH
Sbjct: 343 SISSAATFLITRIYQSQPLVSTMASESG---PAVPALPLQDRVAIVTGASRGIGRGIALH 402

Query: 121 LAALGARVVVNYLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDT 180
           LA LGARVVVNY++SSA A +V A INS+ AA   GS  RA+  +ADVS P++VK+LFD 
Sbjct: 403 LAGLGARVVVNYVASSAEADQVVASINSTSAA---GSSQRAIAWRADVSDPEQVKSLFDA 462

Query: 181 AEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDG 240
           AE+AFGS VHILVNSAGI DPTYPYIADT LEIFD +FSVN  G FLCCKEAA RVK  G
Sbjct: 463 AEQAFGSQVHILVNSAGISDPTYPYIADTPLEIFDHLFSVNTRGCFLCCKEAANRVKRGG 522

Query: 241 GGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDML 300
           GGRII+ISS+AV A + G+GAYTASKAAVEAM K  AKEL GTGIS NC+APGATAT+M 
Sbjct: 523 GGRIILISSTAVVATTAGLGAYTASKAAVEAMAKVAAKELSGTGISVNCIAPGATATEMF 582

Query: 301 YGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 340
           Y G++EE VKKVIEKCP+GR+G P DVA  VGFLASDDGEWINGQ++LVNGGIV
Sbjct: 583 YKGINEEGVKKVIEKCPMGRIGVPKDVASFVGFLASDDGEWINGQVILVNGGIV 630

[Close]

BLAST of MC05g0971 vs. NCBI nr
Match: KAG6597707.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 385 bits (990), Expect = 1.98e-131
Identity = 206/272 (75.74%), Postives = 231/272 (84.93%), Query Frame = 0


Query: 69  MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 128
           MAS  G   PA  ALPLQDRV IVTGASRGIGR +A HLAALGAR+VVNY+SSSA A RV
Sbjct: 1   MASESG---PAMAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRV 60

Query: 129 AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 188
           AAEINSS A    GS  RA+  +ADVS P++VK+LFDTAE+ FG+ VHI+VN AGI DPT
Sbjct: 61  AAEINSSSAT---GSGLRAIAWRADVSDPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPT 120

Query: 189 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 248
           YPYIADTSLEIFD+IFSVN  GSFLCC+EAA RVK  GGGRII+ISS+AVAA + GIGAY
Sbjct: 121 YPYIADTSLEIFDQIFSVNTRGSFLCCREAANRVKRGGGGRIILISSTAVAAATAGIGAY 180

Query: 249 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 308
           TASKAAVEAMTK  AKELRG+ I+ NC+APGATAT+MLYGGMDEEAVK+VIEKCPLGR+G
Sbjct: 181 TASKAAVEAMTKVAAKELRGSRITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVG 240

Query: 309 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 340
            P DVA  VGFLASD+GEWINGQ++LVNGGIV
Sbjct: 241 EPKDVASFVGFLASDEGEWINGQVMLVNGGIV 266

[Close]

BLAST of MC05g0971 vs. ExPASy TrEMBL
Match: A0A6J1DT22 (NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022892 PE=4 SV=1)

HSP 1 Score: 544 bits (1401), Expect = 7.42e-194
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 58  FINLNLLCLPTMASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVN 117
           FINLNLLCLPTMASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVN
Sbjct: 12  FINLNLLCLPTMASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVN 71

Query: 118 YLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHI 177
           YLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHI
Sbjct: 72  YLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHI 131

Query: 178 LVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSA 237
           LVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSA
Sbjct: 132 LVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSA 191

Query: 238 VAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKK 297
           VAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKK
Sbjct: 192 VAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKK 251

Query: 298 VIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 340
           VIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV
Sbjct: 252 VIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 294

[Close]

BLAST of MC05g0971 vs. ExPASy TrEMBL
Match: A0A6J1DQK1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022892 PE=4 SV=1)

HSP 1 Score: 520 bits (1339), Expect = 9.19e-185
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0


Query: 69  MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 128
           MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV
Sbjct: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60

Query: 129 AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 188
           AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT
Sbjct: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120

Query: 189 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 248
           YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY
Sbjct: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180

Query: 249 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 308
           TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG
Sbjct: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240

Query: 309 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 340
           YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV
Sbjct: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 272

[Close]

BLAST of MC05g0971 vs. ExPASy TrEMBL
Match: A0A5A7U7W3 (Photosynthetic NDH subunit of subcomplex B 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00100 PE=4 SV=1)

HSP 1 Score: 461 bits (1186), Expect = 3.52e-156
Identity = 248/354 (70.06%), Postives = 281/354 (79.38%), Query Frame = 0


Query: 1   QGREMFFRVIRMGFEDIYVSSRSSSSNKYGKDYFICTSPASTLVPVTIKPGEEGRGA--- 60
           QGRE+FFRVIRMGFEDIYVSS  S SNK+GKD+FICT PAS LVPVT+KPGE+ +GA   
Sbjct: 283 QGRELFFRVIRMGFEDIYVSSPGSLSNKFGKDHFICTGPASMLVPVTVKPGEQWKGAQYR 342

Query: 61  -------FINLNLL----CLPTMASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALH 120
                  F+   +      + TMAS  G   PA  ALPLQDRVAIVTGASRGIGRG+ALH
Sbjct: 343 SISSAATFLITRIYQSQPLVSTMASESG---PAVPALPLQDRVAIVTGASRGIGRGIALH 402

Query: 121 LAALGARVVVNYLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDT 180
           LA LGARVVVNY++SSA A +V A INS+ AA   GS  RA+  +ADVS P++VK+LFD 
Sbjct: 403 LAGLGARVVVNYVASSAEADQVVASINSTSAA---GSSQRAIAWRADVSDPEQVKSLFDA 462

Query: 181 AEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDG 240
           AE+AFGS VHILVNSAGI DPTYPYIADT LEIFD +FSVN  G FLCCKEAA RVK  G
Sbjct: 463 AEQAFGSQVHILVNSAGISDPTYPYIADTPLEIFDHLFSVNTRGCFLCCKEAANRVKRGG 522

Query: 241 GGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDML 300
           GGRII+ISS+AV A + G+GAYTASKAAVEAM K  AKEL GTGIS NC+APGATAT+M 
Sbjct: 523 GGRIILISSTAVVATTAGLGAYTASKAAVEAMAKVAAKELSGTGISVNCIAPGATATEMF 582

Query: 301 YGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 340
           Y G++EE VKKVIEKCP+GR+G P DVA  VGFLASDDGEWINGQ++LVNGGIV
Sbjct: 583 YKGINEEGVKKVIEKCPMGRIGVPKDVASFVGFLASDDGEWINGQVILVNGGIV 630

[Close]

BLAST of MC05g0971 vs. ExPASy TrEMBL
Match: A0A6J1F321 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439224 PE=4 SV=1)

HSP 1 Score: 384 bits (985), Expect = 5.52e-131
Identity = 206/272 (75.74%), Postives = 230/272 (84.56%), Query Frame = 0


Query: 69  MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 128
           MAS  G   PA  ALPLQDRV IVTGASRGIGR +A HLAALGAR+VVNY+SSSA A RV
Sbjct: 1   MASESG---PAMAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRV 60

Query: 129 AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 188
           AAEINSS A    GS  RA+  +ADVS P++VK+LFDTAE+ FG+ VHI+VN AGI DPT
Sbjct: 61  AAEINSSSAT---GSGLRAIAWRADVSNPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPT 120

Query: 189 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 248
           YPYIADTSLEIFD+IFSVN  GSFLCC+EAA RVK  GGGRII+ISS+AVAA + GIGAY
Sbjct: 121 YPYIADTSLEIFDQIFSVNTRGSFLCCREAAIRVKRGGGGRIILISSTAVAAATAGIGAY 180

Query: 249 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 308
           TASKAAVEAMTK  AKELRG+ I+ NC+APGATAT+MLYGGMDEEAVK+VIEKCPLGR+G
Sbjct: 181 TASKAAVEAMTKVAAKELRGSRITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVG 240

Query: 309 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 340
            P DVA  VGFLASD+GEWINGQ+ LVNGGIV
Sbjct: 241 EPKDVASFVGFLASDEGEWINGQVTLVNGGIV 266

[Close]

BLAST of MC05g0971 vs. ExPASy TrEMBL
Match: A0A0A0LI83 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G843800 PE=4 SV=1)

HSP 1 Score: 381 bits (978), Expect = 6.40e-130
Identity = 204/272 (75.00%), Postives = 227/272 (83.46%), Query Frame = 0


Query: 69  MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 128
           MAS  G   PA  ALPLQDRVAIVTGASRGIGRG+ALHLA LGARVVVNY+SSSA A +V
Sbjct: 1   MASESG---PAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVSSSAEADQV 60

Query: 129 AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 188
            A+INSS AA   GS  RA+  +ADVS P++VK+LFD AE+AFGS VHILVNSAGI DPT
Sbjct: 61  VADINSSSAA---GSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPT 120

Query: 189 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 248
           YPYIA+T LEIFD +FSVN  G FLCCKEAA RVK  GGGRII+ISS+AVAA + G+GAY
Sbjct: 121 YPYIANTPLEIFDHLFSVNTRGCFLCCKEAANRVKRGGGGRIILISSTAVAATTAGLGAY 180

Query: 249 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 308
           TASKAAVEAM K  AKEL GTGIS NC+APGATAT+M Y G+DEE VKKVI+KCP+GRLG
Sbjct: 181 TASKAAVEAMAKVTAKELSGTGISVNCIAPGATATEMFYKGIDEEGVKKVIDKCPMGRLG 240

Query: 309 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 340
            P DVA  VGFLASDDGEWINGQ++LVNGGIV
Sbjct: 241 VPKDVASFVGFLASDDGEWINGQVILVNGGIV 266

[Close]

BLAST of MC05g0971 vs. TAIR 10
Match: AT3G03980.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 308.1 bits (788), Expect = 8.5e-84
Identity = 161/259 (62.16%), Postives = 194/259 (74.90%), Query Frame = 0


Query: 83  LPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPA-ADIA 142
           LPL  RVAIVTG+SRGIGR +A+HLA LGAR+V+NY S +A A RVA+EIN  P   +I 
Sbjct: 12  LPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERVASEINDFPVREEIT 71

Query: 143 GSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFD 202
           G  PRA+  QA+VS+P +VK++FD AE AF +PVHILVNSAGI DP YP IADTS+E FD
Sbjct: 72  GKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDPKYPTIADTSVEDFD 131

Query: 203 RIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKT 262
             FSVN  G+FLC KEAA R+K  GGGRII+++SS   +  PG GAY ASKAAVE M K 
Sbjct: 132 HTFSVNTKGAFLCSKEAANRLKQGGGGRIILLTSSQTRSLKPGFGAYAASKAAVETMVKI 191

Query: 263 LAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLA 322
           LAKEL+GTGI+ANCVAPG  AT+M + G   E V+K+  + P GR+G   DV  LVGFLA
Sbjct: 192 LAKELKGTGITANCVAPGPIATEMFFDGKTPELVEKIAAESPFGRVGEAKDVVPLVGFLA 251

Query: 323 SDDGEWINGQLLLVNGGIV 341
            D GEW+NGQ++ VNGG V
Sbjct: 252 GDGGEWVNGQIIPVNGGYV 270

[Close]

BLAST of MC05g0971 vs. TAIR 10
Match: AT4G13180.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 3.5e-77
Identity = 145/259 (55.98%), Postives = 185/259 (71.43%), Query Frame = 0


Query: 82  ALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPAADIA 141
           +LPL  RVAIVTGA+RG+GR +A+HL +LGARV +NY+SSS+ A  + +E+N S      
Sbjct: 10  SLPLAGRVAIVTGATRGMGREIAIHLHSLGARVTINYVSSSSKAELLVSELNDS------ 69

Query: 142 GSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFD 201
                A+  +ADVS PD++  LFD  E+ FGS VHI+VN AG+ DP YP +++T+LE FD
Sbjct: 70  SQLKSAIAVKADVSDPDQINNLFDQTEQEFGSKVHIVVNCAGVLDPKYPSLSETTLEDFD 129

Query: 202 RIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKT 261
             F++N  GSFLCCKEAAKRV   GGGRIIM+S+S V   +PG G Y ASKAAVE M K 
Sbjct: 130 NTFTINTRGSFLCCKEAAKRVMRGGGGRIIMMSTSMVGGLAPGYGVYAASKAAVETMVKV 189

Query: 262 LAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLA 321
           LAKEL+G+ I+ANCVAPG  AT+M Y G  +E VK +   CP+GR+G   D+  +VGFLA
Sbjct: 190 LAKELKGSRITANCVAPGPVATEMFYAGKSDETVKMLAGACPMGRIGESKDITEIVGFLA 249

Query: 322 SDDGEWINGQLLLVNGGIV 341
            D GEWINGQ++  NGG V
Sbjct: 250 GDGGEWINGQVIRANGGFV 262

[Close]

BLAST of MC05g0971 vs. TAIR 10
Match: AT5G18210.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 285.0 bits (728), Expect = 7.7e-77
Identity = 153/243 (62.96%), Postives = 185/243 (76.13%), Query Frame = 0


Query: 85  LQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPAADIAGSY 144
           L  RVAIVTG+SRGIGR +A+HLA LGA++V+NY + S  A +VAAEINSS     AG+ 
Sbjct: 8   LAGRVAIVTGSSRGIGRAIAIHLAELGAKIVINYTTRSTEADQVAAEINSS-----AGTV 67

Query: 145 PR--AVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDR 204
           P+  AV   AD+S+P ++K+LFD AE+AF SPVHILVNSAGI +P YP IA+T +E FDR
Sbjct: 68  PQPIAVVFLADISEPSQIKSLFDAAEKAFNSPVHILVNSAGILNPNYPTIANTPIEEFDR 127

Query: 205 IFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKTL 264
           IF VN  GSFLCCKEAAKR+K  GGGRII+++SS   A  PG GAYTASKAAVEAM K L
Sbjct: 128 IFKVNTRGSFLCCKEAAKRLKRGGGGRIILLTSSLTEALIPGQGAYTASKAAVEAMVKIL 187

Query: 265 AKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLAS 324
           AKEL+G GI+ANCV+PG  AT+M + G  EE V  +IE+ P GRLG   D+A +VGFLAS
Sbjct: 188 AKELKGLGITANCVSPGPVATEMFFDGKSEETVMNIIERSPFGRLGETKDIASVVGFLAS 245

Query: 325 DDG 326
           D G
Sbjct: 248 DGG 245

[Close]

BLAST of MC05g0971 vs. TAIR 10
Match: AT3G04000.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 6.6e-76
Identity = 150/258 (58.14%), Postives = 189/258 (73.26%), Query Frame = 0


Query: 83  LPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEI--NSSPAADI 142
           L L  RVAIVTG+SRGIGR +A+HLA LGARVVVNY +S   A +VA  I  N S  A++
Sbjct: 12  LCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATAITTNCSKDAEV 71

Query: 143 AGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIF 202
           AG  PR +  +AD+S+P +VK+LFD AE  F SPVHILVNSA I DP +  I+D S+E+F
Sbjct: 72  AGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNHSTISDMSVELF 131

Query: 203 DRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTK 262
           DRI SVN  G+F+C +EAA R+K  GGGRII++S+S V   +   G+YTASKAAVEAM K
Sbjct: 132 DRIISVNTRGAFICAREAANRLKRGGGGRIILLSTSLVQTLNTNYGSYTASKAAVEAMAK 191

Query: 263 TLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFL 322
            LAKEL+GT I+ NCV+PG  AT+M Y G+  E V+KV  +   GR+G   D+A +VGFL
Sbjct: 192 ILAKELKGTEITVNCVSPGPVATEMFYTGLSNEIVEKVKSQNLFGRIGETKDIAPVVGFL 251

Query: 323 ASDDGEWINGQLLLVNGG 339
           ASD GEWINGQ+++ NGG
Sbjct: 252 ASDAGEWINGQVIMANGG 269

[Close]

BLAST of MC05g0971 vs. TAIR 10
Match: AT1G24360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 2.4e-30
Identity = 96/263 (36.50%), Postives = 140/263 (53.23%), Query Frame = 0


Query: 78  PAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPA 137
           P  V   ++  V ++TGASRGIG+ +AL L   G +V+VNY  S+  A  VA +I     
Sbjct: 67  PGEVVQKVESPVVVITGASRGIGKAIALALGKAGCKVLVNYARSAKEAEEVAKQIE---- 126

Query: 138 ADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSL 197
            +  G   +A+T   DVSK  +V A+  TA + +G+ + ++VN+AGI   T   +     
Sbjct: 127 -EYGG---QAITFGGDVSKATDVDAMMKTALDKWGT-IDVVVNNAGITRDT--LLIRMKQ 186

Query: 198 EIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEA 257
             +D + ++N  G FLC + A K +     GRII ISS      + G   Y A+K  V +
Sbjct: 187 SQWDEVIALNLTGVFLCTQAAVKIMMKKKRGRIINISSVVGLIGNIGQANYAAAKGGVIS 246

Query: 258 MTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLV 317
            +KT A+E     I+ N V PG  A+DM    + E+  KK++   PLGR G   +VA LV
Sbjct: 247 FSKTAAREGASRNINVNVVCPGFIASDMT-AELGEDMEKKILGTIPLGRYGKAEEVAGLV 306

Query: 318 GFLA-SDDGEWINGQLLLVNGGI 340
            FLA S    +I GQ   ++GGI
Sbjct: 307 EFLALSPAASYITGQAFTIDGGI 317

The following BLAST results are available for this feature:

BLAST of MC05g0971 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SQR4	1.2e-82	62.16	NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis th...	[more]
Q9SQR2	9.2e-75	58.14	NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q08632	1.1e-72	59.69	Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1	[more]
W3XC32	7.6e-37	38.87	Trihydroxynaphthalene reductase PfmaI OS=Pestalotiopsis fici (strain W106-1 / CG...	[more]
A0A411PQN6	2.2e-36	38.83	Short chain dehydrogenase AgnL6 OS=Paecilomyces divaricatus OX=644132 GN=AgnL6 P...	[more]

BLAST of MC05g0971 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155881.1	1.53e-193	100.00	NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X1 [Momor...	[more]
XP_022155882.1	1.90e-184	100.00	NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X2 [Momor...	[more]
KGN59874.2	2.87e-158	71.19	hypothetical protein Csa_001420 [Cucumis sativus]	[more]
KAA0051280.1	7.28e-156	70.06	photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa] >TYK2998...	[more]
KAG6597707.1	1.98e-131	75.74	NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbi...	[more]

BLAST of MC05g0971 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DT22	7.42e-194	100.00	NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X1 OS=Mom...	[more]
A0A6J1DQK1	9.19e-185	100.00	NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X2 OS=Mom...	[more]
A0A5A7U7W3	3.52e-156	70.06	Photosynthetic NDH subunit of subcomplex B 2 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A6J1F321	5.52e-131	75.74	NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita mosc...	[more]
A0A0A0LI83	6.40e-130	75.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G843800 PE=4 SV=1	[more]

BLAST of MC05g0971 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03980.1	8.5e-84	62.16	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G13180.1	3.5e-77	55.98	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G18210.1	7.7e-77	62.96	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G04000.1	6.6e-76	58.14	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G24360.1	2.4e-30	36.50	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 228..236 score: 48.98 coord: 173..184 score: 46.23 coord: 248..267 score: 43.64
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 269..286 score: 41.12 coord: 248..267 score: 44.01 coord: 303..323 score: 43.46 coord: 222..238 score: 32.16 coord: 89..106 score: 49.13 coord: 173..184 score: 42.85
None	No IPR available	SMART	SM00822	PKS_KR	coord: 88..279 e-value: 0.0043 score: -56.3
None	No IPR available	GENE3D	3.40.50.720		coord: 68..339 e-value: 5.3E-81 score: 273.8
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 94..339 e-value: 1.4E-57 score: 194.9
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 91..287 e-value: 3.2E-11 score: 40.8
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 85..338 e-value: 1.5E-9 score: 35.2
None	No IPR available	PANTHER	PTHR48107	NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED	coord: 78..340
None	No IPR available	PANTHER	PTHR48107:SF19	SHORT-CHAIN DEHYDROGENASE/REDUCTASE	coord: 78..340
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 235..263
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 86..339

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0971.1	MC05g0971.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0006303	double-strand break repair via nonhomologous end joining
biological_process	GO:0042138	meiotic DNA double-strand break formation
biological_process	GO:0097552	mitochondrial double-strand break repair via homologous recombination
biological_process	GO:0007095	mitotic G2 DNA damage checkpoint signaling
biological_process	GO:0000723	telomere maintenance
cellular_component	GO:0030870	Mre11 complex
cellular_component	GO:0035861	site of double-strand break
molecular_function	GO:0008296	3'-5'-exodeoxyribonuclease activity
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0030145	manganese ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0000014	single-stranded DNA endodeoxyribonuclease activity