MC05g0932 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0932
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	O-methyltransferase
Location	MC05: 9517927 .. 9519105 (-)
RNA-Seq Expression	MC05g0932
Synteny	MC05g0932

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGAGATTTCAGAATTGCTTGAAGCTCAAGTCCACATTTGGAATCATGCTTTCAAATATATCAACTCCATGTCTCTAAAATGTGTTGTCGAATTGGGCATACCAGACATTCTCCATACCCATGGCCAGCCCATGAGCCTTTCCGATTTAGTGGAAGCGCTCCATATTCAACCCTCCAAAGCTCAATGCCTTGGCCGCCTCATGCGCCTCCTCGTTCATTCTGGCTTCTTTGCTCGAACACATAGCGAGAATGATGAGCAAGAAACGAAGTACGCTGTCACACCTTCCTCTCGACTTCTCCTTCGTCACAACGACACATTGGAAACCTTACCCTTCTTGTTTCTAATTCTTCATCATGCCATGATGGCTTCGTGGGAGACCATGAGTTGCTGGTTGTGTAGTGTAGATGAGAACTATTCCAGTGCTTTTGAAATGGCGAATGGTAAGTCAATATGGAATTATATTGCAGAGGAACCTGAATTTGGCAATCTATTTCATCGAACAATAGCATATGATTCTCAACTCATTGGGAGAATAGTAACAACGGAGTGTCGAGAGGTGTTTGAGGGGCTGAAGTCGATCGTTGATGTTGGTGGAGGCACAGGCATTATGGCCAAGGCCATCGTGGAAGCATTCCCACACATTACTTACATTGTGTTTGATCTCCCGCAAGTAGTTGCTAACCACCAACAAACCACAAAAAACTTGAGTTTCATTGGAGGAGACATGTTTGAGGCAAAAATTCCACCAACAAATGCAGTTTTACTCAAGGTATGTCTCTCCTCTCGATTTTGTACTATTTCCAAGATTAGTAACAAGTACTTATCCTTTTAAGTTTTAAAATTAGAGTACTTACTATGAGATTTTGATAATCAGTCAGTTTTGTACAACTGGAATGATGAAGAAAGCATTCAGATACTGAAGAATTGTAAAGATGCGATTTTAAGTAATGCTGAGGGTGGAAAAGTGATAATCATAGAGGTGGCGTTGGAATATCAAGTGGAAGACAAGGAGTCAATTGAAACTCAGCTTTGTGGCGATGTGTTGATGATGACTAATTTTGCCATTAGAAAGAGAAACGAGAGAGAATGGAAGAATCTCTTCTTAGCTGCTGGTTTTAGTGACTACAAGATAATTCCTATGCTAGGTTTAAGATCTCTCATCGAGGTGTATCCT

mRNA sequence

ATGGGTGAGATTTCAGAATTGCTTGAAGCTCAAGTCCACATTTGGAATCATGCTTTCAAATATATCAACTCCATGTCTCTAAAATGTGTTGTCGAATTGGGCATACCAGACATTCTCCATACCCATGGCCAGCCCATGAGCCTTTCCGATTTAGTGGAAGCGCTCCATATTCAACCCTCCAAAGCTCAATGCCTTGGCCGCCTCATGCGCCTCCTCGTTCATTCTGGCTTCTTTGCTCGAACACATAGCGAGAATGATGAGCAAGAAACGAAGTACGCTGTCACACCTTCCTCTCGACTTCTCCTTCGTCACAACGACACATTGGAAACCTTACCCTTCTTGTTTCTAATTCTTCATCATGCCATGATGGCTTCGTGGGAGACCATGAGTTGCTGGTTGTGTAGTGTAGATGAGAACTATTCCAGTGCTTTTGAAATGGCGAATGGTAAGTCAATATGGAATTATATTGCAGAGGAACCTGAATTTGGCAATCTATTTCATCGAACAATAGCATATGATTCTCAACTCATTGGGAGAATAGTAACAACGGAGTGTCGAGAGGTGTTTGAGGGGCTGAAGTCGATCGTTGATGTTGGTGGAGGCACAGGCATTATGGCCAAGGCCATCGTGGAAGCATTCCCACACATTACTTACATTGTGTTTGATCTCCCGCAAGTAGTTGCTAACCACCAACAAACCACAAAAAACTTGAGTTTCATTGGAGGAGACATGTTTGAGGCAAAAATTCCACCAACAAATGCAGTTTTACTCAAGTCAGTTTTGTACAACTGGAATGATGAAGAAAGCATTCAGATACTGAAGAATTGTAAAGATGCGATTTTAAGTAATGCTGAGGGTGGAAAAGTGATAATCATAGAGGTGGCGTTGGAATATCAAGTGGAAGACAAGGAGTCAATTGAAACTCAGCTTTGTGGCGATGTGTTGATGATGACTAATTTTGCCATTAGAAAGAGAAACGAGAGAGAATGGAAGAATCTCTTCTTAGCTGCTGGTTTTAGTGACTACAAGATAATTCCTATGCTAGGTTTAAGATCTCTCATCGAGGTGTATCCT

Coding sequence (CDS)

ATGGGTGAGATTTCAGAATTGCTTGAAGCTCAAGTCCACATTTGGAATCATGCTTTCAAATATATCAACTCCATGTCTCTAAAATGTGTTGTCGAATTGGGCATACCAGACATTCTCCATACCCATGGCCAGCCCATGAGCCTTTCCGATTTAGTGGAAGCGCTCCATATTCAACCCTCCAAAGCTCAATGCCTTGGCCGCCTCATGCGCCTCCTCGTTCATTCTGGCTTCTTTGCTCGAACACATAGCGAGAATGATGAGCAAGAAACGAAGTACGCTGTCACACCTTCCTCTCGACTTCTCCTTCGTCACAACGACACATTGGAAACCTTACCCTTCTTGTTTCTAATTCTTCATCATGCCATGATGGCTTCGTGGGAGACCATGAGTTGCTGGTTGTGTAGTGTAGATGAGAACTATTCCAGTGCTTTTGAAATGGCGAATGGTAAGTCAATATGGAATTATATTGCAGAGGAACCTGAATTTGGCAATCTATTTCATCGAACAATAGCATATGATTCTCAACTCATTGGGAGAATAGTAACAACGGAGTGTCGAGAGGTGTTTGAGGGGCTGAAGTCGATCGTTGATGTTGGTGGAGGCACAGGCATTATGGCCAAGGCCATCGTGGAAGCATTCCCACACATTACTTACATTGTGTTTGATCTCCCGCAAGTAGTTGCTAACCACCAACAAACCACAAAAAACTTGAGTTTCATTGGAGGAGACATGTTTGAGGCAAAAATTCCACCAACAAATGCAGTTTTACTCAAGTCAGTTTTGTACAACTGGAATGATGAAGAAAGCATTCAGATACTGAAGAATTGTAAAGATGCGATTTTAAGTAATGCTGAGGGTGGAAAAGTGATAATCATAGAGGTGGCGTTGGAATATCAAGTGGAAGACAAGGAGTCAATTGAAACTCAGCTTTGTGGCGATGTGTTGATGATGACTAATTTTGCCATTAGAAAGAGAAACGAGAGAGAATGGAAGAATCTCTTCTTAGCTGCTGGTTTTAGTGACTACAAGATAATTCCTATGCTAGGTTTAAGATCTCTCATCGAGGTGTATCCT

Protein sequence

MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPSKAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHHAMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRIVTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFIGGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP

Homology

[Close]

BLAST of MC05g0932 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.3e-95
Identity = 184/360 (51.11%), Postives = 248/360 (68.89%), Query Frame = 0


Query: 5   SELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPSKAQC 64
           +E+L AQ HIWN  F +INSMSLKC ++LGIPDI++ HG+PM++S L  AL I   K+ C
Sbjct: 24  TEILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHGKPMTISQLTLALPINRKKSPC 83

Query: 65  LGRLMRLLVHSGFFARTHSENDE----QETKYAVTPSSRLLLRHNDTLETLPFLFLILHH 124
           + RLMR+L+HSGFFA   +E  E    +E  Y +T +S+LLL+ N  +   PFL  +L  
Sbjct: 84  VYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDN-PMSVTPFLLAMLDP 143

Query: 125 AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI 184
            M   W+ +S W  + D    + F+ ANG + W+Y + EP+    F+  +A D++L+  +
Sbjct: 144 VMTKPWDFLSNWFQNGD---PTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLVTSV 203

Query: 185 VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI 244
           V  +C+ VFEG++S+VDVGGGTG +A +I  AFPHI   VFDLP VVA+  Q   NL+F+
Sbjct: 204 VIEKCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVAD-LQGGNNLNFV 263

Query: 245 GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAI-LSNAEGGKVIIIEVALEYQ 304
           GGDMF   +P T  VLLK +L++WNDEES++ILK CK+AI  SN +GGKVIII++ +E +
Sbjct: 264 GGDMF-VDVPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDMKVENE 323

Query: 305 V-EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 359
             ED ES ETQL  D+LMM     R+RNE+EW  LF  AGFS+YKI P+LGLRSLIEVYP
Sbjct: 324 KDEDDESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILGLRSLIEVYP 377

[Close]

BLAST of MC05g0932 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-92
Identity = 173/354 (48.87%), Postives = 237/354 (66.95%), Query Frame = 0


Query: 5   SELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPSKAQC 64
           +ELL AQ H+WNH F +I SMSLKC ++LGIPDI+H HG+PM+L +LV  L + P ++QC
Sbjct: 10  AELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHGKPMTLPELVAKLPVHPKRSQC 69

Query: 65  LGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHHAMMA 124
           + RLMR+LVHSGF A    +  ++E  Y +T +SRLLL  +D+L   P +  +L   +  
Sbjct: 70  VYRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLM-DDSLSIRPLVLAMLDPILTK 129

Query: 125 SWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRIVTTE 184
            W  +S W  + D    + F  A  +S W+Y   EP+  N F+  +A D++L+  ++  E
Sbjct: 130 PWHYLSAWFQNDD---PTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTSVLLKE 189

Query: 185 CREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFIGGDM 244
            + VF GL S+VDVGGGTG +AKAI  AFPH+   V DL  VVA   Q +KNL++  GDM
Sbjct: 190 GQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAG-LQGSKNLNYFAGDM 249

Query: 245 FEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKE 304
           FEA IPP +A+LLK +L++W++EE ++ILK C++AI S   GGKVIII++ +     D +
Sbjct: 250 FEA-IPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQGDYK 309

Query: 305 SIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 359
           S ETQL  D+ MM     R+R+E EW+ LFL AGFS YKI P+LGLRSLIEVYP
Sbjct: 310 STETQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEVYP 357

[Close]

BLAST of MC05g0932 vs. ExPASy Swiss-Prot
Match: Q93WU2 (Eugenol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=EOMT1 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.5e-86
Identity = 165/353 (46.74%), Postives = 230/353 (65.16%), Query Frame = 0


Query: 6   ELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPSKAQCL 65
           +LL+AQVH+WNH + + NSMSLKC ++LGIPDILH HG+PM+LS L++++ I   K QC 
Sbjct: 14  QLLQAQVHVWNHMYAFANSMSLKCAIQLGIPDILHKHGRPMTLSQLLQSIPINKEKTQCF 73

Query: 66  GRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHHAMMAS 125
            RLMR LV+S FF   ++ N+ QE  Y +TP+S LLL+    L   P + ++L       
Sbjct: 74  QRLMRALVNSNFFIEENNSNN-QEVCYWLTPASCLLLK-EAPLTVTPLVQVVLDPTFTNP 133

Query: 126 WETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRIVTTEC 185
           W  MS W     E +++ FE ANG + W  +A EP  G  F   ++ DS+LI  + T + 
Sbjct: 134 WHHMSEWF--THEKHATQFEAANGCTFWEKLANEPSKGRFFDEAMSCDSRLIAHVFTKDY 193

Query: 186 REVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFIGGDMF 245
           + V EG++++VDVGGG G MAKAIVEA P I   V DLP VVA   ++T NL++IGGDMF
Sbjct: 194 KHVIEGIRTLVDVGGGNGTMAKAIVEAMPTIKCTVIDLPHVVAG-LESTDNLNYIGGDMF 253

Query: 246 EAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKES 305
           ++ IP  +A+LLKS++++W+D E ++ILK CKDA++    GGKVIII+V +    +  E 
Sbjct: 254 QS-IPSADAILLKSIIHDWDDVEGLKILKKCKDAVVM---GGKVIIIDVVVGVNHDIDEV 313

Query: 306 IETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 359
           +E QL  D+ MM  F  ++R   EW+ L   AGF  YK+ P  G+RSLIE YP
Sbjct: 314 LEDQLHFDMAMMCYFNAKERTMSEWEKLIYDAGFKSYKLTPAFGVRSLIEAYP 357

[Close]

BLAST of MC05g0932 vs. ExPASy Swiss-Prot
Match: Q93WU3 (Chavicol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=CVOMT1 PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-85
Identity = 164/353 (46.46%), Postives = 230/353 (65.16%), Query Frame = 0


Query: 6   ELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPSKAQCL 65
           +LL+AQ H+WNH + + NSMSLKC ++LGIPDILH H  PM+LS L++A+ I   K+Q  
Sbjct: 14  QLLQAQAHVWNHMYAFANSMSLKCAIQLGIPDILHKHDHPMTLSQLLKAIPINKEKSQSF 73

Query: 66  GRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHHAMMAS 125
            RLMR LV+S FF   +S N  QE  Y +TP+SRLLL+    L   P + ++L       
Sbjct: 74  QRLMRALVNSNFFIEENSNN--QEVCYWLTPASRLLLK-GAPLTVAPLVQVVLDPTFTNP 133

Query: 126 WETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRIVTTEC 185
           W  MS W     EN+++ FE ANG + W  +A +P  G  F   ++ DS+L+  ++T + 
Sbjct: 134 WHYMSEWF--KHENHATQFEAANGCTFWEKLANKPSMGRFFDEAMSCDSRLVAHVLTKDY 193

Query: 186 REVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFIGGDMF 245
           + V +G++++VDVGGG G MAKAIVEA P +   V DLP VVA  + T K LS+IGGDMF
Sbjct: 194 KHVIDGIRTLVDVGGGNGTMAKAIVEAVPTMKCTVLDLPHVVAGLESTDK-LSYIGGDMF 253

Query: 246 EAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKES 305
           ++ IP  +A+LLK ++++W+DEE ++ILK CKDA+     GGKVIII+V +    +  E 
Sbjct: 254 QS-IPSADAILLKFIIHDWDDEEGLKILKRCKDAV---GIGGKVIIIDVVVGVNHDVDEV 313

Query: 306 IETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 359
           +E QL  D+ MM+ F  ++R   EW+ L  AAGF+ YK+ P  G+RSLIE YP
Sbjct: 314 LEDQLHFDMAMMSYFNAKERTMNEWEKLISAAGFTSYKLTPAFGVRSLIEAYP 356

[Close]

BLAST of MC05g0932 vs. ExPASy Swiss-Prot
Match: Q84KK4 (Isoflavone 4'-O-methyltransferase OS=Lotus japonicus OX=34305 GN=HI4'OMT PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.6e-85
Identity = 162/361 (44.88%), Postives = 240/361 (66.48%), Query Frame = 0


Query: 3   EISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPSKA 62
           E +EL +AQ+H++ H + +++SM+LK  +ELGI D++H+HG+P++L +L  AL+++PSK 
Sbjct: 10  EDTELSQAQIHLYKHVYNFVSSMALKSAMELGIADVIHSHGKPITLPELATALNLRPSKI 69

Query: 63  QCLGRLMRLLVHSGFFART---HSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILH 122
             L R +RLL H+GFFA+T     E  E+ET Y +TP S+LL++ N T    P +   LH
Sbjct: 70  GVLHRFLRLLTHNGFFAKTTVSRGEGAEEETAYGLTPPSKLLVKSNSTC-LAPIVKGALH 129

Query: 123 HAMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNL--FHRTIAYDSQLI 182
            + +  W +   W    D    + FE A G+S W ++ +E E   L  F   +A DS + 
Sbjct: 130 PSSLDMWRSSKKWFLE-DNEELTLFESATGESFWEFLNKETESDTLSMFQEAMAADSHMF 189

Query: 183 GRIVTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNL 242
            ++   EC+ VFEGL S+VDV GG G + K I EAFPH+   VFD PQVVAN     +NL
Sbjct: 190 -KLALKECKHVFEGLGSLVDVAGGRGGVTKLIREAFPHVKCTVFDQPQVVAN-LTGDENL 249

Query: 243 SFIGGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALE 302
           +F+GGDMF++ +PP +AVLLK VL++WNDE S++ILKNCK+AI    + GKVIII+++++
Sbjct: 250 NFVGGDMFKS-VPPADAVLLKWVLHDWNDELSLKILKNCKEAISGRGKEGKVIIIDISID 309

Query: 303 YQVEDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVY 359
              +D+E  E +L  D++M+T F  ++R ++EW+ L   AGFS YKI P+ G +SLIEV+
Sbjct: 310 ETSDDRELTELKLDYDLVMLTMFNGKEREKKEWEKLIYDAGFSSYKITPICGFKSLIEVF 365

[Close]

BLAST of MC05g0932 vs. NCBI nr
Match: XP_022152414.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 724 bits (1870), Expect = 1.15e-263
Identity = 358/358 (100.00%), Postives = 358/358 (100.00%), Query Frame = 0


Query: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60
           MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS
Sbjct: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60

Query: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHH 120
           KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHH
Sbjct: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHH 120

Query: 121 AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI 180
           AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI
Sbjct: 121 AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI 180

Query: 181 VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI 240
           VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI
Sbjct: 181 VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI 240

Query: 241 GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQV 300
           GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQV
Sbjct: 241 GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQV 300

Query: 301 EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 358
           EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP
Sbjct: 301 EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 358

[Close]

BLAST of MC05g0932 vs. NCBI nr
Match: XP_022158734.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 559 bits (1440), Expect = 3.49e-198
Identity = 270/358 (75.42%), Postives = 314/358 (87.71%), Query Frame = 0


Query: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60
           MGE +ELL AQ H+WNH FKYINSMSLKCVVELGIPDI+H+HGQPMSLS+LV+ALHIQPS
Sbjct: 1   MGESTELLRAQAHVWNHTFKYINSMSLKCVVELGIPDIIHSHGQPMSLSNLVDALHIQPS 60

Query: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHH 120
           KAQCL RLMRLLVH+GFF +TH +N+++E  YA+TPSSRLLL  N   E +PF+FLIL+ 
Sbjct: 61  KAQCLNRLMRLLVHAGFFIQTHGDNEQEE--YALTPSSRLLLCRNTAFENIPFVFLILNR 120

Query: 121 AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI 180
           AMMA  ETMS WLCSVDE+ +SAFEMANGKSIW+Y+++E +FG LFH+T+ +DSQLIG++
Sbjct: 121 AMMAPLETMSSWLCSVDEHSNSAFEMANGKSIWDYVSQEHDFGKLFHQTMVHDSQLIGKV 180

Query: 181 VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI 240
           VT ECREVFEGLKS+VDVGG TG MAKAI EAFPHIT  VFDLPQV AN QQTTKNLSFI
Sbjct: 181 VTRECREVFEGLKSLVDVGGSTGFMAKAIAEAFPHITCTVFDLPQVAANSQQTTKNLSFI 240

Query: 241 GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQV 300
           GGDMFEAKIPP NAVLLK +L++WNDEESI+ILKNCKDAI S AE GKVI+I++ +E Q+
Sbjct: 241 GGDMFEAKIPPANAVLLKWILHDWNDEESIRILKNCKDAIPSRAEXGKVIVIDMVVENQI 300

Query: 301 EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 358
           EDKES+E QLC D+L+MT++  R+RNEREWKNLFL AGFSDYKII MLGLRSLIEVYP
Sbjct: 301 EDKESVEAQLCCDLLVMTSYNGRERNEREWKNLFLTAGFSDYKIISMLGLRSLIEVYP 356

[Close]

BLAST of MC05g0932 vs. NCBI nr
Match: XP_022152376.1 (trans-resveratrol di-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 516 bits (1328), Expect = 4.02e-181
Identity = 254/358 (70.95%), Postives = 295/358 (82.40%), Query Frame = 0


Query: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60
           MG+  ELL+AQ H+WNHA++Y++SMSLKC VELGIPDI+H+HG+PMSLS+LV ALHIQP 
Sbjct: 1   MGDNIELLKAQAHVWNHAYRYMDSMSLKCAVELGIPDIIHSHGEPMSLSNLVGALHIQPF 60

Query: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHH 120
           KA CLGRLM LLVHSGFF +TH EN EQE KY +T +SRLLLRHN   E +  L LI + 
Sbjct: 61  KAHCLGRLMSLLVHSGFFVQTHDEN-EQEVKYTLTSTSRLLLRHNTPFEHISLLLLIFNK 120

Query: 121 AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI 180
           AMMAS+ETMS W  S DE +S AFE  N KS+W+Y+A+EP+F N+ ++ + YDSQLIGR+
Sbjct: 121 AMMASYETMSSWFRSTDERHSCAFETTNDKSVWDYVAQEPDFSNILNKAMMYDSQLIGRV 180

Query: 181 VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI 240
           VT ECR VF+GLKS+VDVGG  G MAKAI EAFPHIT IVFDLP VV N QQTT+NL+FI
Sbjct: 181 VTRECRRVFKGLKSLVDVGGNRGFMAKAIAEAFPHITCIVFDLPHVVDNLQQTTENLNFI 240

Query: 241 GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQV 300
            G+MFEAKIPP NAVLLK VL++WNDE+SI+ILKNCKDAI S AEGGKVIIIE+  E Q+
Sbjct: 241 AGNMFEAKIPPANAVLLKWVLHDWNDEKSIKILKNCKDAIPSRAEGGKVIIIEMVTENQI 300

Query: 301 EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 358
           EDKESIETQLC DV  M+    R+RNE EWKNLFLAAGFSDYKII MLGLRSLIEVYP
Sbjct: 301 EDKESIETQLCEDVFTMSVVTGRERNESEWKNLFLAAGFSDYKIISMLGLRSLIEVYP 357

[Close]

BLAST of MC05g0932 vs. NCBI nr
Match: XP_038905391.1 (trans-resveratrol di-O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 501 bits (1289), Expect = 3.58e-175
Identity = 257/365 (70.41%), Postives = 294/365 (80.55%), Query Frame = 0


Query: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60
           MGE  ELL+AQ HIWNH FK INSMSLKCVVELGIPDI+H HGQPMSLS LVEALHI PS
Sbjct: 1   MGENKELLQAQAHIWNHTFKCINSMSLKCVVELGIPDIIHNHGQPMSLSQLVEALHIHPS 60

Query: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLL--RHNDTLETLPFLFLIL 120
           KAQCLGRLMRLL+HSGFFA+T      Q   +++TPSSRLLL   H    +T   L L L
Sbjct: 61  KAQCLGRLMRLLLHSGFFAQT------QSGFFSLTPSSRLLLSQNHKTIFDTKTLLLLFL 120

Query: 121 HHAMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPE---FGNLFHRTIAYDSQ 180
              MMA W+TMS WLC+  ++YS+AF+MANGKSIW+Y+ +E E   FG LFH+T+  DSQ
Sbjct: 121 SPLMMAPWQTMSNWLCASTQDYSTAFQMANGKSIWDYVIQEEESYGFGKLFHQTLVCDSQ 180

Query: 181 LIGRIVTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANH--QQT 240
           LIG++VT+EC E+FEGL S+VDVGGG G MAK I+EAFPH+T IV DLPQVVAN   QQ 
Sbjct: 181 LIGKVVTSECGEMFEGLSSLVDVGGGAGTMAKVILEAFPHLTCIVLDLPQVVANQKPQQP 240

Query: 241 TKNLSFIGGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIE 300
            KNL+FI GDMFE KIPP NAVLLKS+L++WNDEESIQILKNCKDAI S AEGG+VIIIE
Sbjct: 241 IKNLNFIEGDMFE-KIPPANAVLLKSILHSWNDEESIQILKNCKDAIPSRAEGGRVIIIE 300

Query: 301 VALEYQVEDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSL 358
           + L+ +  D ESIETQLC DVLMMT F+ R+RNEREWK LFLAAGFSDY IIP+LGLRSL
Sbjct: 301 MVLDQKERDMESIETQLCFDVLMMTTFSGRERNEREWKTLFLAAGFSDYNIIPILGLRSL 358

[Close]

BLAST of MC05g0932 vs. NCBI nr
Match: XP_022982783.1 (probable O-methyltransferase 3 [Cucurbita maxima])

HSP 1 Score: 499 bits (1284), Expect = 1.79e-174
Identity = 257/362 (70.99%), Postives = 299/362 (82.60%), Query Frame = 0


Query: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60
           MGE  ELLEAQ H+WNH F YINSMSL+C+V+LGIPDI+H+HG+PMSLS LVEALHIQPS
Sbjct: 1   MGENRELLEAQAHVWNHTFNYINSMSLRCIVQLGIPDIIHSHGRPMSLSSLVEALHIQPS 60

Query: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHN-DTLETLPFLFLILH 120
           KAQCLGRLMRLLVHSGFFA THS        +++T  SRLLLR N +  +T PFL L+L 
Sbjct: 61  KAQCLGRLMRLLVHSGFFAETHSGF------FSLTTPSRLLLRQNHNAFDTTPFLLLLLS 120

Query: 121 HAMMASWETMSCWLCSVDENYSS-AFEMANGKSIWNYIAEEPE-FGNLFHRTIAYDSQLI 180
              MA WETM+ WLCS D++++S AFEMANG +IW+YIA++P  FGNLFH+ + +DSQLI
Sbjct: 121 PVFMAPWETMNDWLCSDDQDHNSTAFEMANGNTIWDYIAQQPSGFGNLFHQALIHDSQLI 180

Query: 181 GRIVTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANH--QQTTK 240
           G++VT+ECR +FEGL S+VDVGGG G MAKAIVEAFPHI+ IVFDLPQVVAN   QQ  K
Sbjct: 181 GKVVTSECRGMFEGLTSLVDVGGGGGAMAKAIVEAFPHISCIVFDLPQVVANQEPQQAKK 240

Query: 241 NLSFIGGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVA 300
           NL+FIGGDMFE KIP  NA+LLK VL++W+DEESIQILKNCK AI S AEGGKVIIIE+ 
Sbjct: 241 NLNFIGGDMFE-KIPQANALLLKFVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEIV 300

Query: 301 LEYQVEDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIE 357
           LE Q  DKES+ETQLC DV MMT F  R+RNEREWK+LFLAAGFSDY IIP+LGLRS+IE
Sbjct: 301 LEEQ-RDKESVETQLCLDVYMMTTFTGRERNEREWKSLFLAAGFSDYNIIPVLGLRSIIE 354

[Close]

BLAST of MC05g0932 vs. ExPASy TrEMBL
Match: A0A6J1DFY3 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111020151 PE=3 SV=1)

HSP 1 Score: 724 bits (1870), Expect = 5.55e-264
Identity = 358/358 (100.00%), Postives = 358/358 (100.00%), Query Frame = 0


Query: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60
           MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS
Sbjct: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60

Query: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHH 120
           KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHH
Sbjct: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHH 120

Query: 121 AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI 180
           AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI
Sbjct: 121 AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI 180

Query: 181 VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI 240
           VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI
Sbjct: 181 VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI 240

Query: 241 GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQV 300
           GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQV
Sbjct: 241 GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQV 300

Query: 301 EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 358
           EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP
Sbjct: 301 EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 358

[Close]

BLAST of MC05g0932 vs. ExPASy TrEMBL
Match: A0A6J1E096 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111025195 PE=3 SV=1)

HSP 1 Score: 559 bits (1440), Expect = 1.69e-198
Identity = 270/358 (75.42%), Postives = 314/358 (87.71%), Query Frame = 0


Query: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60
           MGE +ELL AQ H+WNH FKYINSMSLKCVVELGIPDI+H+HGQPMSLS+LV+ALHIQPS
Sbjct: 1   MGESTELLRAQAHVWNHTFKYINSMSLKCVVELGIPDIIHSHGQPMSLSNLVDALHIQPS 60

Query: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHH 120
           KAQCL RLMRLLVH+GFF +TH +N+++E  YA+TPSSRLLL  N   E +PF+FLIL+ 
Sbjct: 61  KAQCLNRLMRLLVHAGFFIQTHGDNEQEE--YALTPSSRLLLCRNTAFENIPFVFLILNR 120

Query: 121 AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI 180
           AMMA  ETMS WLCSVDE+ +SAFEMANGKSIW+Y+++E +FG LFH+T+ +DSQLIG++
Sbjct: 121 AMMAPLETMSSWLCSVDEHSNSAFEMANGKSIWDYVSQEHDFGKLFHQTMVHDSQLIGKV 180

Query: 181 VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI 240
           VT ECREVFEGLKS+VDVGG TG MAKAI EAFPHIT  VFDLPQV AN QQTTKNLSFI
Sbjct: 181 VTRECREVFEGLKSLVDVGGSTGFMAKAIAEAFPHITCTVFDLPQVAANSQQTTKNLSFI 240

Query: 241 GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQV 300
           GGDMFEAKIPP NAVLLK +L++WNDEESI+ILKNCKDAI S AE GKVI+I++ +E Q+
Sbjct: 241 GGDMFEAKIPPANAVLLKWILHDWNDEESIRILKNCKDAIPSRAEXGKVIVIDMVVENQI 300

Query: 301 EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 358
           EDKES+E QLC D+L+MT++  R+RNEREWKNLFL AGFSDYKII MLGLRSLIEVYP
Sbjct: 301 EDKESVEAQLCCDLLVMTSYNGRERNEREWKNLFLTAGFSDYKIISMLGLRSLIEVYP 356

[Close]

BLAST of MC05g0932 vs. ExPASy TrEMBL
Match: A0A6J1DHJ0 (trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111020124 PE=3 SV=1)

HSP 1 Score: 516 bits (1328), Expect = 1.95e-181
Identity = 254/358 (70.95%), Postives = 295/358 (82.40%), Query Frame = 0


Query: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60
           MG+  ELL+AQ H+WNHA++Y++SMSLKC VELGIPDI+H+HG+PMSLS+LV ALHIQP 
Sbjct: 1   MGDNIELLKAQAHVWNHAYRYMDSMSLKCAVELGIPDIIHSHGEPMSLSNLVGALHIQPF 60

Query: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHH 120
           KA CLGRLM LLVHSGFF +TH EN EQE KY +T +SRLLLRHN   E +  L LI + 
Sbjct: 61  KAHCLGRLMSLLVHSGFFVQTHDEN-EQEVKYTLTSTSRLLLRHNTPFEHISLLLLIFNK 120

Query: 121 AMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRI 180
           AMMAS+ETMS W  S DE +S AFE  N KS+W+Y+A+EP+F N+ ++ + YDSQLIGR+
Sbjct: 121 AMMASYETMSSWFRSTDERHSCAFETTNDKSVWDYVAQEPDFSNILNKAMMYDSQLIGRV 180

Query: 181 VTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFI 240
           VT ECR VF+GLKS+VDVGG  G MAKAI EAFPHIT IVFDLP VV N QQTT+NL+FI
Sbjct: 181 VTRECRRVFKGLKSLVDVGGNRGFMAKAIAEAFPHITCIVFDLPHVVDNLQQTTENLNFI 240

Query: 241 GGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQV 300
            G+MFEAKIPP NAVLLK VL++WNDE+SI+ILKNCKDAI S AEGGKVIIIE+  E Q+
Sbjct: 241 AGNMFEAKIPPANAVLLKWVLHDWNDEKSIKILKNCKDAIPSRAEGGKVIIIEMVTENQI 300

Query: 301 EDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVYP 358
           EDKESIETQLC DV  M+    R+RNE EWKNLFLAAGFSDYKII MLGLRSLIEVYP
Sbjct: 301 EDKESIETQLCEDVFTMSVVTGRERNESEWKNLFLAAGFSDYKIISMLGLRSLIEVYP 357

[Close]

BLAST of MC05g0932 vs. ExPASy TrEMBL
Match: A0A6J1J5U8 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111481541 PE=3 SV=1)

HSP 1 Score: 499 bits (1284), Expect = 8.64e-175
Identity = 257/362 (70.99%), Postives = 299/362 (82.60%), Query Frame = 0


Query: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60
           MGE  ELLEAQ H+WNH F YINSMSL+C+V+LGIPDI+H+HG+PMSLS LVEALHIQPS
Sbjct: 1   MGENRELLEAQAHVWNHTFNYINSMSLRCIVQLGIPDIIHSHGRPMSLSSLVEALHIQPS 60

Query: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHN-DTLETLPFLFLILH 120
           KAQCLGRLMRLLVHSGFFA THS        +++T  SRLLLR N +  +T PFL L+L 
Sbjct: 61  KAQCLGRLMRLLVHSGFFAETHSGF------FSLTTPSRLLLRQNHNAFDTTPFLLLLLS 120

Query: 121 HAMMASWETMSCWLCSVDENYSS-AFEMANGKSIWNYIAEEPE-FGNLFHRTIAYDSQLI 180
              MA WETM+ WLCS D++++S AFEMANG +IW+YIA++P  FGNLFH+ + +DSQLI
Sbjct: 121 PVFMAPWETMNDWLCSDDQDHNSTAFEMANGNTIWDYIAQQPSGFGNLFHQALIHDSQLI 180

Query: 181 GRIVTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANH--QQTTK 240
           G++VT+ECR +FEGL S+VDVGGG G MAKAIVEAFPHI+ IVFDLPQVVAN   QQ  K
Sbjct: 181 GKVVTSECRGMFEGLTSLVDVGGGGGAMAKAIVEAFPHISCIVFDLPQVVANQEPQQAKK 240

Query: 241 NLSFIGGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVA 300
           NL+FIGGDMFE KIP  NA+LLK VL++W+DEESIQILKNCK AI S AEGGKVIIIE+ 
Sbjct: 241 NLNFIGGDMFE-KIPQANALLLKFVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEIV 300

Query: 301 LEYQVEDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIE 357
           LE Q  DKES+ETQLC DV MMT F  R+RNEREWK+LFLAAGFSDY IIP+LGLRS+IE
Sbjct: 301 LEEQ-RDKESVETQLCLDVYMMTTFTGRERNEREWKSLFLAAGFSDYNIIPVLGLRSIIE 354

[Close]

BLAST of MC05g0932 vs. ExPASy TrEMBL
Match: A0A6J1F479 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111442010 PE=3 SV=1)

HSP 1 Score: 493 bits (1270), Expect = 1.16e-172
Identity = 255/362 (70.44%), Postives = 297/362 (82.04%), Query Frame = 0


Query: 1   MGEISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQPS 60
           MGE  ELLEA+ H+WNH F YINSMSL+CVV+LGIPDI+H+HG+PMSLS LVEALHIQPS
Sbjct: 1   MGENRELLEAEAHVWNHTFNYINSMSLRCVVQLGIPDIIHSHGRPMSLSSLVEALHIQPS 60

Query: 61  KAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHN-DTLETLPFLFLILH 120
           KAQCLGRLMRLLVHSGFFA THS        +++T  SRLLLR N +  +T P L L+  
Sbjct: 61  KAQCLGRLMRLLVHSGFFAETHSGF------FSLTTPSRLLLRQNHNAFDTTPLLLLLTS 120

Query: 121 HAMMASWETMSCWLCSVDENYSS-AFEMANGKSIWNYIAEEPE-FGNLFHRTIAYDSQLI 180
              MA WETMS WLCS D++++S AFEMANG +IW+YIA++P  FGNLFH+T+ +DSQLI
Sbjct: 121 PVFMAPWETMSNWLCSDDKDHNSTAFEMANGNTIWDYIAQQPSGFGNLFHQTLIHDSQLI 180

Query: 181 GRIVTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANH--QQTTK 240
           G++VT+ECR +FEGL S+VDVGGG G MAKAIVEAFPHI+ IVFDLPQVVA+   QQ  K
Sbjct: 181 GKVVTSECRGMFEGLTSLVDVGGGAGAMAKAIVEAFPHISCIVFDLPQVVASQEPQQAKK 240

Query: 241 NLSFIGGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVA 300
           NL+FIGGDMFE KIP  NA+LLK VL++W+DEESIQILKNCK AI S AEGGKVIIIE+ 
Sbjct: 241 NLNFIGGDMFE-KIPQANALLLKLVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEMV 300

Query: 301 LEYQVEDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIE 357
            E Q  DKES+ETQLC DV MMT F  R+RNEREWK+LFLAAGFSDY IIP+LGLRS+IE
Sbjct: 301 FEEQ-RDKESVETQLCCDVYMMTTFTGRERNEREWKSLFLAAGFSDYNIIPVLGLRSIIE 354

[Close]

BLAST of MC05g0932 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 185.3 bits (469), Expect = 8.8e-47
Identity = 114/366 (31.15%), Postives = 197/366 (53.83%), Query Frame = 0


Query: 4   ISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTH--GQPMSLSDLVEALHIQPSK 63
           + E  +A + IW + F + +  + KC ++L IP+ +  H   QP++L++L  A+   PS 
Sbjct: 23  LDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSH 82

Query: 64  AQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTP-SSRLLLRHNDTLETLPFLFLILHH 123
              L R+MR LVH G F    ++ D   T Y  TP S RL++   D     PF+      
Sbjct: 83  ---LRRIMRFLVHQGIFKEIPTK-DGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTP 142

Query: 124 AMMASWETMSCWLCS-VDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGR 183
            M+A W  +S  + S V+ +    F+  +GK +W++  + P   ++ +  +A D++ +  
Sbjct: 143 EMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVVP 202

Query: 184 IVTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSF 243
            V   C  +F+G+ ++VDVGGGTG     +V+ FP I    FDLP V+    +    +  
Sbjct: 203 RVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVI-EVAEVLDGVEN 262

Query: 244 IGGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALE-- 303
           + GDMF++ IP  +A+ +K VL++W D++ I+ILKNCK+A+  N   GKV+I+E  +   
Sbjct: 263 VEGDMFDS-IPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNI--GKVLIVESVIGEN 322

Query: 304 -----YQVEDKESIETQLCGDVLMMTNFAI-RKRNEREWKNLFLAAGFSDYKIIPMLGLR 358
                    D++    +L  D++MM + +  ++R  +EW  +   AGF+ Y++  +  ++
Sbjct: 323 KKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQ 380

[Close]

BLAST of MC05g0932 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 163.3 bits (412), Expect = 3.6e-40
Identity = 109/366 (29.78%), Postives = 181/366 (49.45%), Query Frame = 0


Query: 4   ISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTH--GQPMSLSDLVEALHIQPSK 63
           + E  +A + IW + F + +  + KC ++L IP+ +  H   QP++LS+L  A+   PS 
Sbjct: 9   LDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSH 68

Query: 64  AQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHHA 123
              L R+MR LVH G F    ++ D   T Y  TP SR ++                   
Sbjct: 69  ---LRRIMRFLVHQGLFKEVPTK-DGLATGYTNTPLSRRMM------------------- 128

Query: 124 MMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRIV 183
                                     +GK +W +  +      L +  +A D++ +   V
Sbjct: 129 ----------------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDARRVVPRV 188

Query: 184 TTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFIG 243
              C+ +F+G+ ++VDVGGGTG     +V+ FP I    FDLP V+    Q    +  + 
Sbjct: 189 AGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVI-EVAQVLDGVENVE 248

Query: 244 GDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVAL----- 303
           GDMF++ IP ++AV++K VL++W D++ I+ILKNCK+A+L N   GKV+I+E  +     
Sbjct: 249 GDMFDS-IPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNI--GKVLIVECVIGEKKN 308

Query: 304 ----EYQVEDKESIETQLCGDVLMMTNFAI-RKRNEREWKNLFLAAGFSDYKIIPMLGLR 358
               E + +  E +  QL  D++MM + +  ++R  +EW  +   AGF+ Y++     ++
Sbjct: 309 TMIAEERDDKLEHVRLQL--DMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQ 323

[Close]

BLAST of MC05g0932 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 149.4 bits (376), Expect = 5.3e-36
Identity = 106/347 (30.55%), Postives = 187/347 (53.89%), Query Frame = 0


Query: 25  MSLKCVVELGIPDIL--HTHGQPMSLSDLVEALHIQPSKAQC---LGRLMRLLVHSGFF- 84
           M LK  +ELG+ D +   ++G  +S S++  +L  +P+  +    L R++RLLV      
Sbjct: 44  MVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILK 103

Query: 85  -----ARTHSENDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILH-HAMMASWETMSCW 144
                +R + +  + E  YA  P  +  L+ +D   +L  L L+LH   ++ +W  +   
Sbjct: 104 CCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSSLLLLLHSQVILKTWTNLKDV 163

Query: 145 LCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRIVTTECREVFEGL 204
           +    + +SSA +M     ++ YI+ + +F  LFHR ++  S ++ + V  E R  FE +
Sbjct: 164 ILEGKDAFSSAHDM----RLFEYISSDDQFSKLFHRAMSESSTMVMKKVLEEYRG-FEDV 223

Query: 205 KSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFIGGDMFEAKIPPT 264
            ++VDVGGG G +   I   +PHI  + FDL QV+         +  + GDMF  ++P  
Sbjct: 224 NTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQVL-TQAPFYPGVKHVSGDMF-IEVPKG 283

Query: 265 NAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKE-SIETQLC 324
           +A+ +K +L++W DE+ I+ILKNC  ++    E GKVII+E+    + +  + S  T L 
Sbjct: 284 DAIFMKWILHDWGDEDCIKILKNCWKSL---PEKGKVIIVEMITPMEPKPNDFSCNTVLG 343

Query: 325 GDVLMMTNFA-IRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVY 358
            D+LM+T  +  ++R+  +++NL  A+GF   +II +    S+IE +
Sbjct: 344 MDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYSVIEFH 380

[Close]

BLAST of MC05g0932 vs. TAIR 10
Match: AT5G53810.1 (O-methyltransferase family protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.5e-33
Identity = 109/348 (31.32%), Postives = 180/348 (51.72%), Query Frame = 0


Query: 25  MSLKCVVELGIPDILHTHGQP---MSLSDLVEALHIQPSKAQC---LGRLMRLLV-HSGF 84
           M LK  +ELG+ D + T G     +S S++   L  +P   +    L R++R LV HS  
Sbjct: 40  MVLKAALELGVIDTITTVGGGDLWLSPSEIALRLPTKPCNLEAPALLDRMLRFLVSHSVL 99

Query: 85  FARTHSENDEQ----ETKYAVTPSSRLLLRHNDTLE-TLPFLFLI-LHHAMMASWETMSC 144
             RT  E + Q    E  YA  P  + LL  +D +  +   LF++ L    + +W  +  
Sbjct: 100 KCRTVIEENGQTGKVERVYAAEPVCKYLLNKSDDVSGSFASLFMLDLSDVFIKTWTHLE- 159

Query: 145 WLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRIVTTECREVFEG 204
               V      AF  A+G  ++ YI  +  FG +F+R +   S ++   V  +  E F+ 
Sbjct: 160 ---DVILEGRDAFSSAHGMKLFEYIQADERFGKVFNRAMLESSTMVTEKV-LKFYEGFKD 219

Query: 205 LKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFIGGDMFEAKIPP 264
           +K++VDVGGG G     I   +PH+  I FDL  V+AN   +   ++ + GDMF  KIP 
Sbjct: 220 VKTLVDVGGGLGNTLGLITSKYPHLIGINFDLAPVLAN-AHSYPGVNHVAGDMF-IKIPK 279

Query: 265 TNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKESIETQLC 324
            +A+ +K +L++W DE+ + ILKNC  ++    E GK+II+E+    + +  +     + 
Sbjct: 280 GDAIFMKWILHDWTDEQCVAILKNCWKSL---EENGKLIIVEMVTPVEAKSGDICSNIVF 339

Query: 325 G-DVLMMTNFA-IRKRNEREWKNLFLAAGFSDYKIIPMLGLRSLIEVY 358
           G D+ M+T  +  ++R+  E++NL  A+GFS   I+  +   S+IE+Y
Sbjct: 340 GMDMTMLTQCSGGKERDLYEFENLAYASGFSRCAIVCAVYPFSVIEIY 377

[Close]

BLAST of MC05g0932 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-32
Identity = 95/341 (27.86%), Postives = 168/341 (49.27%), Query Frame = 0


Query: 9   EAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQ-PSKAQCLGR 68
           EA +     A   +  M+LK  +EL + +I+  +G PMS +++   L  + P     L R
Sbjct: 18  EAALFAMQLASASVLPMALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDR 77

Query: 69  LMRLLVHSGFFARTHSE--NDEQETKYAVTPSSRLLLRHNDTLETLPFLFLILHHAMMAS 128
           ++RLL        ++ +   D  E  Y + P  + L ++ D +       +     +M S
Sbjct: 78  ILRLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMES 137

Query: 129 WETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTIAYDSQLIGRIVTTEC 188
           W  +   +     +    F  A G S + Y   +P F  +F+  ++  S +  + +  E 
Sbjct: 138 WYHLKDAIL----DGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKI-LET 197

Query: 189 REVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVANHQQTTKNLSFIGGDMF 248
            + FEGL S+VDVGGG G   K IV  +P++  I FDLP V+ +   +   +  +GGDMF
Sbjct: 198 YKGFEGLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIED-APSHPGIEHVGGDMF 257

Query: 249 EAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKES 308
              +P  +A+ +K + ++W+DE  ++ LKNC +++    E GKVI+ E  L    +   S
Sbjct: 258 -VSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESL---PEDGKVILAECILPETPDSSLS 317

Query: 309 IETQLCGDVLMMT-NFAIRKRNEREWKNLFLAAGFSDYKII 346
            +  +  D +M+  N   ++R E+E++ L  A+GF   K++
Sbjct: 318 TKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVV 348

The following BLAST results are available for this feature:

BLAST of MC05g0932 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B0ZB57	1.3e-95	51.11	Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1	[more]
B6VJS4	1.8e-92	48.87	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1...	[more]
Q93WU2	2.5e-86	46.74	Eugenol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=EOMT1 PE=1 SV=1	[more]
Q93WU3	1.2e-85	46.46	Chavicol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=CVOMT1 PE=1 SV=1	[more]
Q84KK4	4.6e-85	44.88	Isoflavone 4'-O-methyltransferase OS=Lotus japonicus OX=34305 GN=HI4'OMT PE=1 SV...	[more]

BLAST of MC05g0932 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152414.1	1.15e-263	100.00	probable O-methyltransferase 3 [Momordica charantia]	[more]
XP_022158734.1	3.49e-198	75.42	probable O-methyltransferase 3 [Momordica charantia]	[more]
XP_022152376.1	4.02e-181	70.95	trans-resveratrol di-O-methyltransferase-like [Momordica charantia]	[more]
XP_038905391.1	3.58e-175	70.41	trans-resveratrol di-O-methyltransferase-like [Benincasa hispida]	[more]
XP_022982783.1	1.79e-174	70.99	probable O-methyltransferase 3 [Cucurbita maxima]	[more]

BLAST of MC05g0932 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DFY3	5.55e-264	100.00	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111020151 PE...	[more]
A0A6J1E096	1.69e-198	75.42	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111025195 PE...	[more]
A0A6J1DHJ0	1.95e-181	70.95	trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1J5U8	8.64e-175	70.99	probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111481541 PE=3 ...	[more]
A0A6J1F479	1.16e-172	70.44	probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111442010 PE=...	[more]

BLAST of MC05g0932 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	8.8e-47	31.15	O-methyltransferase family protein	[more]
AT4G35150.1	3.6e-40	29.78	O-methyltransferase family protein	[more]
AT1G77530.1	5.3e-36	30.55	O-methyltransferase family protein	[more]
AT5G53810.1	1.5e-33	31.32	O-methyltransferase family protein	[more]
AT5G54160.1	1.2e-32	27.86	O-methyltransferase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 142..340 e-value: 9.3E-50 score: 168.9
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 2..101 e-value: 3.9E-41 score: 141.0
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 105..358 e-value: 2.5E-71 score: 241.8
None	No IPR available	PANTHER	PTHR11746:SF250	O-METHYLTRANSFERASE	coord: 5..358
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 195..292 e-value: 0.00288244 score: 35.0983
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 25..73 e-value: 3.0E-18 score: 65.6
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 2..358 e-value: 4.4E-113 score: 375.8
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 5..358
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 15..358 score: 72.78035
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 110..357
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 5..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0932.1	MC05g0932.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity