MC05g0920 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0920
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAuxin-responsive GH3 family protein
LocationMC05: 9049633 .. 9052593 (+)
RNA-Seq ExpressionMC05g0920
SyntenyMC05g0920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCATAGCCTCTACTTCCCTTACATTTCCTCCCTTTTGTTTTTTGTTTTTGGTGTAAATTTTTATTTCCAAAAATCCCCTTCAGAATCTGTTATTTAATCATTTCCCCTTCTGCCTCCCCCCCTGCCATTGCAGATTGAAGATACCTGGAAAACACAAAAAGGGATGTACCTTTAATACAACACCACCATGGTTAATAGTTTATAACCTGATCATGGAACCAAAAACCAAGCTTCTTCTTCTTTTTCATCTTCTTTTTCTGTTCCATTCGTTTTCTTGATACCCATCTTGTAAATTTTGATCTCATCCAAATCCCCAAACTCTCTCTGTTTGATCATTTGATCTCCTCTAGAGATGTTGCCAAATTTTGACCCCAACGACAATGAATCTGGCCTGAAACTGCTTGAAGATCTGACACAAAATGCAAGGCAAATACAAGAGCAGGTGATTCAGAAGATTCTCTCACAAAATGCCAACACAGAATATCTCAAGAACTTCATCAATAATGGCTGCTGCAGCCACTCCTCCCCTGACCTCAAACTTTTCAAAAACTCTGTCCCTGTGGTCAACTACGAAGACATTAAGCCTTACATCGAGAGAATTGCCAATGGGGAGCCCTCCAACATAATCTCAGCTCAACCCATCACTGAGCTTCTCACAAGGTAACTAAATTCAATTCATTCTGTACTGGAACTTTTCTTTTTCTTGTAACATCATCAATGGAAAAATGGCAGCTCCGGAACTTCTGGAGGCCAGCCGAAGATGATGCCTTCCACTGCTGAAGACTTGGACAGAAAGACATTCTTCTACAACCTCCTCGTCCCTATCATGAACAAGTACTAAACTTTGACACAGAACCAAAAAAAACATCTCCTTTATGAATAAAACAGTGCAATTTTTCACTTTTTTTTCTTTCTGTTTTGGCTTTTTTTAGGCATGTGGGTGGTTTGGAGGAAGGAAAAGGGATGTACTTGTTGTTTATAAAGCCAGAGATCAGCACCCCATCTGGGCTAATGGCGAGGCCAGTGCTGACAAGCTACTACAAGAGCAAGAACTTCAGAAACAGGCCTTTCAATAGGTATAATGTGTACACAAGCCCTGATGAGACAATTTTATGCTCTGACAGTAAGCAGAGTATGTACTGCCAATTGCTTTGTGGGTTGATTCAGAGGGACGAGGTTTTGAGGGTGGGCGCAGTTTTTGCCTCGGCTTTCCTCAGGGCCATAAAATTCTTAGAAGATTACTGGAAAGAGATGAGCCATAACATAAGAAGTGGCTGTGTCAGCGAGTGGATCACTGACCCCAGCTGCAGAAGCTCTGTTTCTTTGATCCTCACGAAGCCCAATGAGGAGTTGGCTGATTTGATTGATGCCATGTGCGGGGAGAAATCGTGGGAAGGGATTATTAAGAAGATTTGGGCCAAGACCAAATACATTGAGGTTATTGTGACAGGGTCTATGGCTCAGTATATTCCCACATTGGAATTTTACAGTGGGGGGCTTCCATTGGTTTCTACCATGTATGCTTCCTCTGAGTGCTATTTTGGGATTAATTTTAGTCCTTTGAGTAAGCCTGAGGATGTTTCGTATACCCTTCTGCCAAATATGGCCTTCTTTGAGTTTCTGCCTGTGGAGAGAAATGACGGAGGAGAATTGGCTCGTTCGGGCCCCCACCGGAATGGGGGCGCAGTTCGGGACGACGGCGACGAGATGGGGAAACTTGAAACAGTGGACCTCGTCGACGTGGAACTCGGTCGCTATTACGAGCTTGTGGTGACCACTTTCACGGGTGAAACTCCTTTCCCTCTTTTTGTGTTTTTGGTTGCTTTTTTGGGGATCTGACCCACATCATTTGTTAGAGTTCATTAGGGGAAGAAAAAGACTATTCAACTCAACCAGATTCAAACAGGGGTTTTGTTTTCCTGGCTTAAATTTGTTTGTTGTTGGTTAGTTAATCTAATCATGCTTAATAATGTTCATGTGGGGGTGCGTGTGTGTGTGTTCAGGGCTATACAGATACAGAGTTGGTGACATTCTAAAGGTAACTGGATTCCACAACAATGCTCCTCAGTTCCGCTTCATTCATCGTCGGAACGTCGTGTTGAGCATCGACACCGACAAGACGAATGAAGACGATCTGTTGAAGGCAATAACAAACGCAAAATTGCTGGTTGAGCCACTGGGCATTCTGTTGACAGAGTACAGTAGCTATGCAGACACCAGCTCAATCCCAGGGCATTATGTGCTGTTTTGGGAGCTGAAGAAGCGAGGCGGTGGGGGCGAGAATGCTGCAGCGACCGCAGTCGACTTGGGAGTGATGGAGGAATGCTGCTCAAGTATAGAAGAAAGTCTGGATTCTGTGTACAGAAGATGCAGGAGCAAGGATAAATCCATAGGGCCATTGGAGATAAGGGTGGTGAAAGGAGGAGCTTTTGATGCACTGATGGATTATTGTGTGTCACAAGGCTCTTCTGTGAACCAATACAAGACACCAAGATGCATCAAATCGGAGGAGGCCATTAGGATTCTTGATTCCAGAGTGGTGGGAAGATTCTTCAGCAGGAAAACCCCATTTTGGGAGCCTTTCAGAGTGGACTGCTCTAAATGACTGACTCCTTGTAGTGTGTTAATGTGGAATCTTCCTCATTTTCTCTCTCTCTTTCTCTTTCTTCTTTTTCTGTCTGGCTGGGAACCAGTGCCCTTTCAAACCGTGATCTGGTTAGATCCTTTTGTTCCCAAATTTGAATTGATTGTGTAAAGCCGAGGGCTCTTTATGCATTTAAAACTGTGTATTACTTTTCGGATTTTGTGTGGGAACTATTTGGACAAAAGCAACAGTTGGTTTAGCACAACAGATACATTAGAATTTTCAACTTCTTCAATTAAGTCCCTAAACTTTAAAAATGGTTAACTTTGTTGAGTTTTACAAACAAAGTCCCATATTGACTAAAAAGAGGTTTGATC

mRNA sequence

CCATAGCCTCTACTTCCCTTACATTTCCTCCCTTTTGTTTTTTGTTTTTGGTGTAAATTTTTATTTCCAAAAATCCCCTTCAGAATCTGTTATTTAATCATTTCCCCTTCTGCCTCCCCCCCTGCCATTGCAGATTGAAGATACCTGGAAAACACAAAAAGGGATGTACCTTTAATACAACACCACCATGGTTAATAGTTTATAACCTGATCATGGAACCAAAAACCAAGCTTCTTCTTCTTTTTCATCTTCTTTTTCTGTTCCATTCGTTTTCTTGATACCCATCTTGTAAATTTTGATCTCATCCAAATCCCCAAACTCTCTCTGTTTGATCATTTGATCTCCTCTAGAGATGTTGCCAAATTTTGACCCCAACGACAATGAATCTGGCCTGAAACTGCTTGAAGATCTGACACAAAATGCAAGGCAAATACAAGAGCAGGTGATTCAGAAGATTCTCTCACAAAATGCCAACACAGAATATCTCAAGAACTTCATCAATAATGGCTGCTGCAGCCACTCCTCCCCTGACCTCAAACTTTTCAAAAACTCTGTCCCTGTGGTCAACTACGAAGACATTAAGCCTTACATCGAGAGAATTGCCAATGGGGAGCCCTCCAACATAATCTCAGCTCAACCCATCACTGAGCTTCTCACAAGCTCCGGAACTTCTGGAGGCCAGCCGAAGATGATGCCTTCCACTGCTGAAGACTTGGACAGAAAGACATTCTTCTACAACCTCCTCGTCCCTATCATGAACAAGCATGTGGGTGGTTTGGAGGAAGGAAAAGGGATGTACTTGTTGTTTATAAAGCCAGAGATCAGCACCCCATCTGGGCTAATGGCGAGGCCAGTGCTGACAAGCTACTACAAGAGCAAGAACTTCAGAAACAGGCCTTTCAATAGGTATAATGTGTACACAAGCCCTGATGAGACAATTTTATGCTCTGACAGTAAGCAGAGTATGTACTGCCAATTGCTTTGTGGGTTGATTCAGAGGGACGAGGTTTTGAGGGTGGGCGCAGTTTTTGCCTCGGCTTTCCTCAGGGCCATAAAATTCTTAGAAGATTACTGGAAAGAGATGAGCCATAACATAAGAAGTGGCTGTGTCAGCGAGTGGATCACTGACCCCAGCTGCAGAAGCTCTGTTTCTTTGATCCTCACGAAGCCCAATGAGGAGTTGGCTGATTTGATTGATGCCATGTGCGGGGAGAAATCGTGGGAAGGGATTATTAAGAAGATTTGGGCCAAGACCAAATACATTGAGGTTATTGTGACAGGGTCTATGGCTCAGTATATTCCCACATTGGAATTTTACAGTGGGGGGCTTCCATTGGTTTCTACCATGTATGCTTCCTCTGAGTGCTATTTTGGGATTAATTTTAGTCCTTTGAGTAAGCCTGAGGATGTTTCGTATACCCTTCTGCCAAATATGGCCTTCTTTGAGTTTCTGCCTGTGGAGAGAAATGACGGAGGAGAATTGGCTCGTTCGGGCCCCCACCGGAATGGGGGCGCAGTTCGGGACGACGGCGACGAGATGGGGAAACTTGAAACAGTGGACCTCGTCGACGTGGAACTCGGTCGCTATTACGAGCTTGTGGTGACCACTTTCACGGGGCTATACAGATACAGAGTTGGTGACATTCTAAAGGTAACTGGATTCCACAACAATGCTCCTCAGTTCCGCTTCATTCATCGTCGGAACGTCGTGTTGAGCATCGACACCGACAAGACGAATGAAGACGATCTGTTGAAGGCAATAACAAACGCAAAATTGCTGGTTGAGCCACTGGGCATTCTGTTGACAGAGTACAGTAGCTATGCAGACACCAGCTCAATCCCAGGGCATTATGTGCTGTTTTGGGAGCTGAAGAAGCGAGGCGGTGGGGGCGAGAATGCTGCAGCGACCGCAGTCGACTTGGGAGTGATGGAGGAATGCTGCTCAAGTATAGAAGAAAGTCTGGATTCTGTGTACAGAAGATGCAGGAGCAAGGATAAATCCATAGGGCCATTGGAGATAAGGGTGGTGAAAGGAGGAGCTTTTGATGCACTGATGGATTATTGTGTGTCACAAGGCTCTTCTGTGAACCAATACAAGACACCAAGATGCATCAAATCGGAGGAGGCCATTAGGATTCTTGATTCCAGAGTGGTGGGAAGATTCTTCAGCAGGAAAACCCCATTTTGGGAGCCTTTCAGAGTGGACTGCTCTAAATGACTGACTCCTTGTAGTGTGTTAATGTGGAATCTTCCTCATTTTCTCTCTCTCTTTCTCTTTCTTCTTTTTCTGTCTGGCTGGGAACCAGTGCCCTTTCAAACCGTGATCTGGTTAGATCCTTTTGTTCCCAAATTTGAATTGATTGTGTAAAGCCGAGGGCTCTTTATGCATTTAAAACTGTGTATTACTTTTCGGATTTTGTGTGGGAACTATTTGGACAAAAGCAACAGTTGGTTTAGCACAACAGATACATTAGAATTTTCAACTTCTTCAATTAAGTCCCTAAACTTTAAAAATGGTTAACTTTGTTGAGTTTTACAAACAAAGTCCCATATTGACTAAAAAGAGGTTTGATC

Coding sequence (CDS)

ATGTTGCCAAATTTTGACCCCAACGACAATGAATCTGGCCTGAAACTGCTTGAAGATCTGACACAAAATGCAAGGCAAATACAAGAGCAGGTGATTCAGAAGATTCTCTCACAAAATGCCAACACAGAATATCTCAAGAACTTCATCAATAATGGCTGCTGCAGCCACTCCTCCCCTGACCTCAAACTTTTCAAAAACTCTGTCCCTGTGGTCAACTACGAAGACATTAAGCCTTACATCGAGAGAATTGCCAATGGGGAGCCCTCCAACATAATCTCAGCTCAACCCATCACTGAGCTTCTCACAAGCTCCGGAACTTCTGGAGGCCAGCCGAAGATGATGCCTTCCACTGCTGAAGACTTGGACAGAAAGACATTCTTCTACAACCTCCTCGTCCCTATCATGAACAAGCATGTGGGTGGTTTGGAGGAAGGAAAAGGGATGTACTTGTTGTTTATAAAGCCAGAGATCAGCACCCCATCTGGGCTAATGGCGAGGCCAGTGCTGACAAGCTACTACAAGAGCAAGAACTTCAGAAACAGGCCTTTCAATAGGTATAATGTGTACACAAGCCCTGATGAGACAATTTTATGCTCTGACAGTAAGCAGAGTATGTACTGCCAATTGCTTTGTGGGTTGATTCAGAGGGACGAGGTTTTGAGGGTGGGCGCAGTTTTTGCCTCGGCTTTCCTCAGGGCCATAAAATTCTTAGAAGATTACTGGAAAGAGATGAGCCATAACATAAGAAGTGGCTGTGTCAGCGAGTGGATCACTGACCCCAGCTGCAGAAGCTCTGTTTCTTTGATCCTCACGAAGCCCAATGAGGAGTTGGCTGATTTGATTGATGCCATGTGCGGGGAGAAATCGTGGGAAGGGATTATTAAGAAGATTTGGGCCAAGACCAAATACATTGAGGTTATTGTGACAGGGTCTATGGCTCAGTATATTCCCACATTGGAATTTTACAGTGGGGGGCTTCCATTGGTTTCTACCATGTATGCTTCCTCTGAGTGCTATTTTGGGATTAATTTTAGTCCTTTGAGTAAGCCTGAGGATGTTTCGTATACCCTTCTGCCAAATATGGCCTTCTTTGAGTTTCTGCCTGTGGAGAGAAATGACGGAGGAGAATTGGCTCGTTCGGGCCCCCACCGGAATGGGGGCGCAGTTCGGGACGACGGCGACGAGATGGGGAAACTTGAAACAGTGGACCTCGTCGACGTGGAACTCGGTCGCTATTACGAGCTTGTGGTGACCACTTTCACGGGGCTATACAGATACAGAGTTGGTGACATTCTAAAGGTAACTGGATTCCACAACAATGCTCCTCAGTTCCGCTTCATTCATCGTCGGAACGTCGTGTTGAGCATCGACACCGACAAGACGAATGAAGACGATCTGTTGAAGGCAATAACAAACGCAAAATTGCTGGTTGAGCCACTGGGCATTCTGTTGACAGAGTACAGTAGCTATGCAGACACCAGCTCAATCCCAGGGCATTATGTGCTGTTTTGGGAGCTGAAGAAGCGAGGCGGTGGGGGCGAGAATGCTGCAGCGACCGCAGTCGACTTGGGAGTGATGGAGGAATGCTGCTCAAGTATAGAAGAAAGTCTGGATTCTGTGTACAGAAGATGCAGGAGCAAGGATAAATCCATAGGGCCATTGGAGATAAGGGTGGTGAAAGGAGGAGCTTTTGATGCACTGATGGATTATTGTGTGTCACAAGGCTCTTCTGTGAACCAATACAAGACACCAAGATGCATCAAATCGGAGGAGGCCATTAGGATTCTTGATTCCAGAGTGGTGGGAAGATTCTTCAGCAGGAAAACCCCATTTTGGGAGCCTTTCAGAGTGGACTGCTCTAAATGA

Protein sequence

MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPDLKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDYWKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAKTKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPNMAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTFTGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPLGILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSVYRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRVVGRFFSRKTPFWEPFRVDCSK
Homology
BLAST of MC05g0920 vs. ExPASy Swiss-Prot
Match: Q9FZ87 (Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana OX=3702 GN=GH3.17 PE=1 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 6.7e-257
Identity = 434/620 (70.00%), Postives = 517/620 (83.39%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           M+P++DPND E+GLKLLEDLT NA  IQ+QV+ +ILSQN+ T+YL+ F++      +  +
Sbjct: 1   MIPSYDPNDTEAGLKLLEDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLD----GEADKN 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
            + FKN VPVVNY+D+KP+I+RIA+GE S+I+SAQPITELLTSSGTS G+PK+MPSTAE+
Sbjct: 61  QQSFKNKVPVVNYDDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEE 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           L+RKTFFY++LVPIMNK+V GL+EGKGMYLLFIKPEI TPSGLMARPVLTSYYKS++FRN
Sbjct: 121 LERKTFFYSMLVPIMNKYVDGLDEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFN+YNVYTSPD+TILC DSKQSMYCQLLCGL+QR  VLRVGAVFASAFLRA+KFLED+
Sbjct: 181 RPFNKYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDH 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           +KE+  +IR+G V+ WITD SCR SV  IL  PN+ELAD I++ C EKSWEGI+++IW K
Sbjct: 241 YKELCADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAEKSWEGILRRIWPK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
            KY+EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGIN +PL  P DVSYTLLPN
Sbjct: 301 AKYVEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MA+FEFLPV+     E+     H N     DD D + +   V+LV+VE+G+YYE+V+TTF
Sbjct: 361 MAYFEFLPVDDKSHEEI-HFATHSN---TDDDDDALKEDLIVNLVNVEVGQYYEIVITTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKL--LVE 480
           TGLYRYRVGDILKVTGFHN APQFRF+ RRNVVLSIDTDKT+E+DLL A+T AKL  L  
Sbjct: 421 TGLYRYRVGDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQH 480

Query: 481 PLGILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLD 540
           P  +LLTEY+SYADTSSIPGHYVLFWELK R     +     +D   ME+CCS +E+ LD
Sbjct: 481 PSSLLLTEYTSYADTSSIPGHYVLFWELKPR----HSNDPPKLDDKTMEDCCSEVEDCLD 540

Query: 541 SVYRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDS 600
            VYRRCR++DKSIGPLEIRVV  G FD+LMD+CVSQGSS+NQYKTPRC+KS  A+ ILDS
Sbjct: 541 YVYRRCRNRDKSIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDS 600

Query: 601 RVVGRFFSRKTPFWEPFRVD 619
           RV+GRFFS++ P WEP  +D
Sbjct: 601 RVIGRFFSKRVPQWEPLGLD 608

BLAST of MC05g0920 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 6.8e-201
Identity = 351/605 (58.02%), Postives = 448/605 (74.05%), Query Frame = 0

Query: 10  NESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPDLKLFKNSVP 69
           N++ L+ +ED+T NA  +Q +V+++ILS+NA+ EYLK     G       D + FK+ +P
Sbjct: 21  NKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEG-----RTDRETFKHIMP 80

Query: 70  VVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYN 129
           VV YEDI+P I RIANG+ S ++ + PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+
Sbjct: 81  VVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 140

Query: 130 LLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVY 189
           LL+P+M++ V GL++GKGMY LFIK E  TP GL ARPVLTSYYKS +F+NRP++ Y  Y
Sbjct: 141 LLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNY 200

Query: 190 TSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDYWKEMSHNIR 249
           TSP++TILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W E++ +IR
Sbjct: 201 TSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIR 260

Query: 250 SGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAKTKYIEVIVT 309
           +G +S  ITD S R +V  IL KP+ +LAD +++ C + SW+GII ++W  TKY++VIVT
Sbjct: 261 TGTLSSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVT 320

Query: 310 GSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPNMAFFEFLPV 369
           G+M+QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+PNMA+FEFLPV
Sbjct: 321 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPV 380

Query: 370 ERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTFTGLYRYRVG 429
            RN G   + S P            E  + E VDLVDV+LG+ YELVVTT+ GLYRYRVG
Sbjct: 381 HRNSGVTSSISLPKAL--------TEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVG 440

Query: 430 DILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPLGILLTEYSS 489
           D+L V GF NNAPQF FI R+NVVLSID+DKT+E +L  A+ NA   + P    L+EY+S
Sbjct: 441 DVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTS 500

Query: 490 YADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSVYRRCRSKDK 549
           YADTSSIPGHYVLFWEL   G        T +   V E+CC +IEESL+SVYR+ R  DK
Sbjct: 501 YADTSSIPGHYVLFWELCLNGN-------TPIPPSVFEDCCLTIEESLNSVYRQGRVSDK 560

Query: 550 SIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRVVGRFFSRKT 609
           SIGPLEI++V+ G FD LMDY +S G+S+NQYKTPRC+K    I +L+SRVV  +FS K 
Sbjct: 561 SIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKC 604

Query: 610 PFWEP 615
           P W P
Sbjct: 621 PKWSP 604

BLAST of MC05g0920 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.5e-200
Identity = 351/605 (58.02%), Postives = 450/605 (74.38%), Query Frame = 0

Query: 10  NESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPDLKLFKNSVP 69
           N+  L+L+E+LT NA Q+Q QV+++IL++NA+ EYL+    NG       D + FKN +P
Sbjct: 21  NKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNG-----RTDRETFKNIMP 80

Query: 70  VVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYN 129
           V+ YEDI+P I RIANG+ S I+S++PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+
Sbjct: 81  VITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 140

Query: 130 LLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVY 189
           LL+P+M++ V GLE GKGMY LFIK E  TP GL ARPVLTSYYKS +F+ RP++ Y  Y
Sbjct: 141 LLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNY 200

Query: 190 TSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDYWKEMSHNIR 249
           TSP+ETILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W E+  +IR
Sbjct: 201 TSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIR 260

Query: 250 SGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAKTKYIEVIVT 309
           +G +S  ITDPS R +V+ IL KP+ +LAD ++  C + SW+GII ++W  TKY++VIVT
Sbjct: 261 TGTLSSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVT 320

Query: 310 GSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPNMAFFEFLPV 369
           G+M+QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+P+MA+FEFLPV
Sbjct: 321 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPV 380

Query: 370 ERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTFTGLYRYRVG 429
            RN+G   + + P            E  + E VDLVDV+LG+ YELVVTT+ GL RYRVG
Sbjct: 381 HRNNGVTNSINLPKAL--------TEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVG 440

Query: 430 DILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPLGILLTEYSS 489
           D+L+VTGF N APQF FI R+NVVLSID+DKT+E +L  A+ NA   + P    L+EY+S
Sbjct: 441 DLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTS 500

Query: 490 YADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSVYRRCRSKDK 549
           YADTSSIPGHYVLFWEL   G        T +   V E+CC ++EES ++VYR+ R  DK
Sbjct: 501 YADTSSIPGHYVLFWELCLDGN-------TPIPPSVFEDCCLAVEESFNTVYRQGRVSDK 560

Query: 550 SIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRVVGRFFSRKT 609
           SIGPLEI++V+ G FD LMDY +S G+S+NQYKTPRC+K    I +L+SRVV  +FS K 
Sbjct: 561 SIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKC 604

Query: 610 PFWEP 615
           P W P
Sbjct: 621 PKWVP 604

BLAST of MC05g0920 vs. ExPASy Swiss-Prot
Match: P0C0M3 (Probable indole-3-acetic acid-amido synthetase GH3.11 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.11 PE=2 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 2.1e-194
Identity = 335/598 (56.02%), Postives = 440/598 (73.58%), Query Frame = 0

Query: 14  LKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPDLKLFKNSVPVVNY 73
           L+ LE LT NA++ QE ++ KIL +N  TEYL  F+N       S ++  FK  VPVV Y
Sbjct: 14  LEELEMLTVNAKEAQELILTKILERNQATEYLSKFMN------GSTNISAFKRHVPVVTY 73

Query: 74  EDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 133
           + + PYI RIA GE S+I+  + I ELL SSGTS G+P++MPS  +DLDR+T+ Y+L++P
Sbjct: 74  DKVHPYILRIATGEESSILCGEYILELLRSSGTSRGEPRLMPSILKDLDRRTYLYSLIMP 133

Query: 134 IMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 193
           IMNK++ GL EGK MYLLF+K E  T SG+  R VLTSYYKS +F +R  + YN YTSPD
Sbjct: 134 IMNKYISGLGEGKAMYLLFVKAETLTDSGIPVRSVLTSYYKSPHFLHRKHDLYNNYTSPD 193

Query: 194 ETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDYWKEMSHNIRSGCV 253
           E ILC DS+QSMYCQLLCGL++R  VLR+GAVFASAFLR+I FLE +W+++ ++IR G +
Sbjct: 194 EVILCPDSQQSMYCQLLCGLVERQHVLRIGAVFASAFLRSISFLEQHWRDLVNDIRIGQL 253

Query: 254 SEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAKTKYIEVIVTGSMA 313
           +  IT P+CR ++   L  PN ELAD ++A+C   SW+GI+ ++W   KYIE ++TG+MA
Sbjct: 254 NSSITSPACRLAMLNFLALPNPELADQVEAICSCGSWKGILGRLWPNVKYIEAVLTGTMA 313

Query: 314 QYIPTLEFYSGG-LPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPNMAFFEFLPVERN 373
           QYIP LEFY GG +P V TMYASSE YFG+N SPL  P DVSYT+LPNMA+FEF+P+E  
Sbjct: 314 QYIPMLEFYGGGAIPFVCTMYASSESYFGVNLSPLCSPADVSYTILPNMAYFEFIPLE-- 373

Query: 374 DGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTFTGLYRYRVGDIL 433
                       +G  + D  + +   + V LVDV++G YYELVVTTF+GLYRYRVGD+L
Sbjct: 374 ------------DGLRLTDHEEVIENDKLVSLVDVKVGCYYELVVTTFSGLYRYRVGDVL 433

Query: 434 KVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPLGILLTEYSSYAD 493
           +VTGF+N APQF+FI RRNV+LSID+DKTNE+DL  ++T AK ++E    LL EY+SY D
Sbjct: 434 QVTGFYNRAPQFKFICRRNVILSIDSDKTNEEDLHNSVTTAKKILENQNYLLLEYTSYTD 493

Query: 494 TSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSVYRRCRSKDKSIG 553
            S++PGHYVLFWE+K       +     +D  ++E CC+++EESLD VYRRCR+ D+SIG
Sbjct: 494 ISTVPGHYVLFWEIKST----HDERPAPLDAQLLESCCAAVEESLDYVYRRCRAHDRSIG 553

Query: 554 PLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRVVGRFFSRKTP 611
           PLEIR+V+ GAFDALMD  VS GSS+NQYKTPRCI+S  A+++L+S+V+  FFS + P
Sbjct: 554 PLEIRLVEAGAFDALMDLLVSHGSSINQYKTPRCIESSLALKLLNSKVIACFFSPQDP 587

BLAST of MC05g0920 vs. ExPASy Swiss-Prot
Match: O82243 (Putative indole-3-acetic acid-amido synthetase GH3.9 OS=Arabidopsis thaliana OX=3702 GN=GH3.9 PE=2 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 1.4e-193
Identity = 339/610 (55.57%), Postives = 447/610 (73.28%), Query Frame = 0

Query: 9   DNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPDLKLFKNSV 68
           D++S LK LE +T  A ++Q+ +++ IL +N +TEYL  ++N       S D+  FK +V
Sbjct: 7   DHDSVLKELERITSKAAEVQDNILRGILERNKDTEYLSKYMN------GSKDVLEFKRAV 66

Query: 69  PVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFY 128
           P++ Y+DI PYI+RIANGE S++I+   ITE+L SSGTS G+PK+MP+  EDLDR+TF Y
Sbjct: 67  PIIIYKDIYPYIQRIANGEDSSLITGHSITEILCSSGTSAGEPKLMPTIPEDLDRRTFLY 126

Query: 129 NLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNV 188
           NL++PI+NK++ GL++GK MYL F+K E STP GL  R VLTSYYKSK+F+ RP++ +N 
Sbjct: 127 NLIIPIVNKYITGLDKGKAMYLNFVKAETSTPCGLPIRAVLTSYYKSKHFQCRPYDPFND 186

Query: 189 YTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDYWKEMSHNI 248
            TSP +TILC DS QSMYCQLL GLI R +V+R+GAVFASAFLRAI +LE  W ++  +I
Sbjct: 187 LTSPIQTILCEDSNQSMYCQLLAGLIHRHKVMRLGAVFASAFLRAISYLEKKWSQLCEDI 246

Query: 249 RSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAKTKYIEVIV 308
           R+G ++  ITDP C+ ++S +L  PN ELA  I+ +CG  SW+GI+ ++W K K+IE +V
Sbjct: 247 RTGSLNPMITDPGCQMAMSCLLMSPNPELASEIEEICGRSSWKGILCQLWPKAKFIEAVV 306

Query: 309 TGSMAQYIPTLEFYS-GGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPNMAFFEFL 368
           TGSMAQYIP LEF+S G +PLV  MYASSE YFG+N  PLSKP DV +TLLPNM +FEF+
Sbjct: 307 TGSMAQYIPALEFFSQGKIPLVCPMYASSETYFGVNVEPLSKPSDVVFTLLPNMCYFEFI 366

Query: 369 PVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTFTGLYRYR 428
           P+ +N  G L+            DD +++   + VDLV+V+LGRYYELVVTTF GLYRYR
Sbjct: 367 PLGKN--GTLSFD---------LDDDEQVPCDKVVDLVNVKLGRYYELVVTTFAGLYRYR 426

Query: 429 VGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPLG--ILLT 488
           +GD+L+V GF+N APQFRFI RRNVVLSID DKTNE+DL ++IT AK   + LG    L 
Sbjct: 427 IGDVLQVAGFYNGAPQFRFICRRNVVLSIDLDKTNEEDLHRSITLAK---KKLGSNAFLA 486

Query: 489 EYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSVYRRCR 548
           EY+SYADTSS+PGHYVLFWE++             ++  +MEECC ++EE LD +YR+CR
Sbjct: 487 EYTSYADTSSVPGHYVLFWEIQGH-----------LEPKLMEECCVAVEEELDYIYRQCR 546

Query: 549 SKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEA-IRILDSRVVGRF 608
           +K++SIG LEIRVVK G F+ LMD  +SQG S NQYKTPRC+KS  A  ++L+  V+  F
Sbjct: 547 TKERSIGALEIRVVKPGTFEKLMDLIISQGGSFNQYKTPRCVKSNSATFKLLNGHVMASF 585

Query: 609 FSRKTPFWEP 615
           FS + P W P
Sbjct: 607 FSPRDPTWVP 585

BLAST of MC05g0920 vs. NCBI nr
Match: XP_022153513.1 (indole-3-acetic acid-amido synthetase GH3.17-like [Momordica charantia])

HSP 1 Score: 1253 bits (3242), Expect = 0.0
Identity = 621/621 (100.00%), Postives = 621/621 (100.00%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD
Sbjct: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED
Sbjct: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY
Sbjct: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK
Sbjct: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF
Sbjct: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480
           TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL
Sbjct: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480

Query: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540
           GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV
Sbjct: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540

Query: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600
           YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600

Query: 601 VGRFFSRKTPFWEPFRVDCSK 621
           VGRFFSRKTPFWEPFRVDCSK
Sbjct: 601 VGRFFSRKTPFWEPFRVDCSK 621

BLAST of MC05g0920 vs. NCBI nr
Match: KAG6581972.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018391.1 Indole-3-acetic acid-amido synthetase GH3.17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1054 bits (2725), Expect = 0.0
Identity = 530/621 (85.35%), Postives = 568/621 (91.47%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           MLP+FDPNDNE GLKLLEDLTQNARQIQEQVI+KIL+QNANTEYLK+FIN GC    SPD
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFIN-GC---HSPD 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           + LFKN+VPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGG PKMMPSTAED
Sbjct: 61  VHLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGHPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVP+MNK+V GLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFNRYNVYTSPDETILC+DS+QSMYCQLLCGL+QRD+VLRVGAVFASAFLRAIKFLED+
Sbjct: 181 RPFNRYNVYTSPDETILCADSRQSMYCQLLCGLVQRDDVLRVGAVFASAFLRAIKFLEDH 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           WKEMS NIR G VS+WITDP+CR+SVSL+LTKPN  LADLIDAMCGEK WEGIIKKIW K
Sbjct: 241 WKEMSDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MAFFEFLPVE+N+G EL+    H NGG+V        + ETVDLVDV+LG YYELVVTTF
Sbjct: 361 MAFFEFLPVEKNNG-ELS----HCNGGSV----PPADEFETVDLVDVKLGHYYELVVTTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480
           TGLYRYRVGDIL VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AKLL+EPL
Sbjct: 421 TGLYRYRVGDILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPL 480

Query: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540
           G+LLTEY+SYADT SIPGHYVLFWELK RGG     +   ++  +MEECCSS+E+SLDSV
Sbjct: 481 GVLLTEYTSYADTGSIPGHYVLFWELKNRGG---CESLGELESSLMEECCSSVEQSLDSV 540

Query: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600
           YRRCRSKDKSIGPLEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAIRILDSR 
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRA 600

Query: 601 VGRFFSRKTPFWEPFRVDCSK 621
           VGRFFSRKTPFWEPFR   S+
Sbjct: 601 VGRFFSRKTPFWEPFRAQYSQ 605

BLAST of MC05g0920 vs. NCBI nr
Match: XP_023528388.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1052 bits (2720), Expect = 0.0
Identity = 529/621 (85.19%), Postives = 568/621 (91.47%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           MLP+FDPND+E GLKLLEDLTQNARQIQEQVI+KIL+QNANTEYLK+FIN GC    SPD
Sbjct: 1   MLPSFDPNDDEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFIN-GC---HSPD 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           + LFKN+VPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGG PKMMPSTAED
Sbjct: 61  VHLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGHPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVP+MNK+V GLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFNRYNVYTSPDETILC+DS+QSMYCQLLCGL+QRD+VLRVGAVFASAFLRAIKFLED+
Sbjct: 181 RPFNRYNVYTSPDETILCADSRQSMYCQLLCGLVQRDDVLRVGAVFASAFLRAIKFLEDH 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           WKEMS NIR G VS+WITDP+CR+SVSL+LTKPN  LADLIDAMCGEK WEGIIKKIW K
Sbjct: 241 WKEMSDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MAFFEFLPVE+N+G EL+    H NGG+V        + ETVDLVDV+LG YYELVVTTF
Sbjct: 361 MAFFEFLPVEKNNG-ELS----HCNGGSV----PPADEFETVDLVDVKLGHYYELVVTTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480
           TGLYRYRVGDIL VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AKLL+EPL
Sbjct: 421 TGLYRYRVGDILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPL 480

Query: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540
           G+LLTEY+SYADT SIPGHYVLFWELK RGG     +   ++  +MEECCSS+E+SLDSV
Sbjct: 481 GVLLTEYTSYADTGSIPGHYVLFWELKNRGG---CESLGELESSLMEECCSSVEQSLDSV 540

Query: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600
           YRRCRSKDKSIGPLEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAIRILDSR 
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRA 600

Query: 601 VGRFFSRKTPFWEPFRVDCSK 621
           VGRFFSRKTPFWEPFR   S+
Sbjct: 601 VGRFFSRKTPFWEPFRAQYSQ 605

BLAST of MC05g0920 vs. NCBI nr
Match: XP_022980842.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita maxima])

HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 529/621 (85.19%), Postives = 568/621 (91.47%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           ML +FDPNDNE GLKLLEDLTQNARQIQEQVI+KIL+QNANTEYLK+FIN GC    SPD
Sbjct: 1   MLSSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFIN-GC---HSPD 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           + LFKN+VPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED
Sbjct: 61  VHLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVP+MNK+V GLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFNRYNVYTSPDETILC+DS+QSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLED+
Sbjct: 181 RPFNRYNVYTSPDETILCADSRQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDH 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           WKEMS NIR G VS+WITDP+CR+SVSL+LTKPN  LADLID+MCGEK WEGIIKKIW K
Sbjct: 241 WKEMSDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDSMCGEKLWEGIIKKIWPK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MAFFEFLPVE+N+G EL+    H NGG+V        + ETVDLVDV+LG YYELVVTTF
Sbjct: 361 MAFFEFLPVEKNNG-ELS----HCNGGSV----PPADEFETVDLVDVKLGHYYELVVTTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480
           TGLYRYRVGDIL VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AKLL+EPL
Sbjct: 421 TGLYRYRVGDILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPL 480

Query: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540
           G+LLTEY+SYADT SIPGHYVLFWELK RGG     +   ++  +MEECCSS+E+SLDSV
Sbjct: 481 GVLLTEYTSYADTGSIPGHYVLFWELKNRGG---CESLGELESSLMEECCSSVEQSLDSV 540

Query: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600
           YRRCRSKDKSIGPLEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAI+ILDSR 
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRA 600

Query: 601 VGRFFSRKTPFWEPFRVDCSK 621
           VGRFFSRKTPFWEPFR   S+
Sbjct: 601 VGRFFSRKTPFWEPFRAQYSQ 605

BLAST of MC05g0920 vs. NCBI nr
Match: XP_022955484.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita moschata])

HSP 1 Score: 1050 bits (2714), Expect = 0.0
Identity = 528/621 (85.02%), Postives = 567/621 (91.30%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           MLP+FDPNDNE GLKLLEDLTQNARQIQEQVI+KIL+QNANTEYLK+FIN GC    SPD
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFIN-GC---HSPD 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           + LFKN+VPVVNYEDIKPYIERIANGEPSNIISAQPI ELLTSSGTSGG PKMMPSTAED
Sbjct: 61  VHLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPIIELLTSSGTSGGHPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVP+MNK+V GLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFNRYNVYTSPDETILC+DS+QSMYCQLLCGL+QR++VLRVGAVFASAFLRAIKFLED+
Sbjct: 181 RPFNRYNVYTSPDETILCADSRQSMYCQLLCGLVQREDVLRVGAVFASAFLRAIKFLEDH 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           WKEMS NIR G VS+WITDP+CR+SVSL+LTKPN  LADLIDAMCGEK WEGIIKKIW K
Sbjct: 241 WKEMSDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MAFFEFLPVE+N+G EL+    H NGG+V        + ETVDLVDV+LG YYELVVTTF
Sbjct: 361 MAFFEFLPVEKNNG-ELS----HCNGGSV----PPADEFETVDLVDVKLGHYYELVVTTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480
           TGLYRYRVGDIL VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AKLL+EPL
Sbjct: 421 TGLYRYRVGDILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPL 480

Query: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540
           G+LLTEY+SYADT SIPGHYVLFWELK RGG     +   ++  +MEECCSS+E+SLDSV
Sbjct: 481 GVLLTEYTSYADTGSIPGHYVLFWELKNRGG---CESLGELESSLMEECCSSVEKSLDSV 540

Query: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600
           YRRCRSKDKSIGPLEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAIRILDSR 
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRA 600

Query: 601 VGRFFSRKTPFWEPFRVDCSK 621
           VGRFFSRKTPFWEPFR   S+
Sbjct: 601 VGRFFSRKTPFWEPFRAQYSQ 605

BLAST of MC05g0920 vs. ExPASy TrEMBL
Match: A0A6J1DHN6 (indole-3-acetic acid-amido synthetase GH3.17-like OS=Momordica charantia OX=3673 GN=LOC111020995 PE=3 SV=1)

HSP 1 Score: 1253 bits (3242), Expect = 0.0
Identity = 621/621 (100.00%), Postives = 621/621 (100.00%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD
Sbjct: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED
Sbjct: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY
Sbjct: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK
Sbjct: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF
Sbjct: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480
           TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL
Sbjct: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480

Query: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540
           GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV
Sbjct: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540

Query: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600
           YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600

Query: 601 VGRFFSRKTPFWEPFRVDCSK 621
           VGRFFSRKTPFWEPFRVDCSK
Sbjct: 601 VGRFFSRKTPFWEPFRVDCSK 621

BLAST of MC05g0920 vs. ExPASy TrEMBL
Match: A0A6J1ISD7 (indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita maxima OX=3661 GN=LOC111480114 PE=3 SV=1)

HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 529/621 (85.19%), Postives = 568/621 (91.47%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           ML +FDPNDNE GLKLLEDLTQNARQIQEQVI+KIL+QNANTEYLK+FIN GC    SPD
Sbjct: 1   MLSSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFIN-GC---HSPD 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           + LFKN+VPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED
Sbjct: 61  VHLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVP+MNK+V GLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFNRYNVYTSPDETILC+DS+QSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLED+
Sbjct: 181 RPFNRYNVYTSPDETILCADSRQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDH 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           WKEMS NIR G VS+WITDP+CR+SVSL+LTKPN  LADLID+MCGEK WEGIIKKIW K
Sbjct: 241 WKEMSDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDSMCGEKLWEGIIKKIWPK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MAFFEFLPVE+N+G EL+    H NGG+V        + ETVDLVDV+LG YYELVVTTF
Sbjct: 361 MAFFEFLPVEKNNG-ELS----HCNGGSV----PPADEFETVDLVDVKLGHYYELVVTTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480
           TGLYRYRVGDIL VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AKLL+EPL
Sbjct: 421 TGLYRYRVGDILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPL 480

Query: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540
           G+LLTEY+SYADT SIPGHYVLFWELK RGG     +   ++  +MEECCSS+E+SLDSV
Sbjct: 481 GVLLTEYTSYADTGSIPGHYVLFWELKNRGG---CESLGELESSLMEECCSSVEQSLDSV 540

Query: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600
           YRRCRSKDKSIGPLEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAI+ILDSR 
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRA 600

Query: 601 VGRFFSRKTPFWEPFRVDCSK 621
           VGRFFSRKTPFWEPFR   S+
Sbjct: 601 VGRFFSRKTPFWEPFRAQYSQ 605

BLAST of MC05g0920 vs. ExPASy TrEMBL
Match: A0A6J1GWE7 (indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita moschata OX=3662 GN=LOC111457496 PE=3 SV=1)

HSP 1 Score: 1050 bits (2714), Expect = 0.0
Identity = 528/621 (85.02%), Postives = 567/621 (91.30%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           MLP+FDPNDNE GLKLLEDLTQNARQIQEQVI+KIL+QNANTEYLK+FIN GC    SPD
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFIN-GC---HSPD 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           + LFKN+VPVVNYEDIKPYIERIANGEPSNIISAQPI ELLTSSGTSGG PKMMPSTAED
Sbjct: 61  VHLFKNTVPVVNYEDIKPYIERIANGEPSNIISAQPIIELLTSSGTSGGHPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVP+MNK+V GLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFNRYNVYTSPDETILC+DS+QSMYCQLLCGL+QR++VLRVGAVFASAFLRAIKFLED+
Sbjct: 181 RPFNRYNVYTSPDETILCADSRQSMYCQLLCGLVQREDVLRVGAVFASAFLRAIKFLEDH 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           WKEMS NIR G VS+WITDP+CR+SVSL+LTKPN  LADLIDAMCGEK WEGIIKKIW K
Sbjct: 241 WKEMSDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINF+PLSKP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MAFFEFLPVE+N+G EL+    H NGG+V        + ETVDLVDV+LG YYELVVTTF
Sbjct: 361 MAFFEFLPVEKNNG-ELS----HCNGGSV----PPADEFETVDLVDVKLGHYYELVVTTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480
           TGLYRYRVGDIL VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AKLL+EPL
Sbjct: 421 TGLYRYRVGDILMVTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPL 480

Query: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540
           G+LLTEY+SYADT SIPGHYVLFWELK RGG     +   ++  +MEECCSS+E+SLDSV
Sbjct: 481 GVLLTEYTSYADTGSIPGHYVLFWELKNRGG---CESLGELESSLMEECCSSVEKSLDSV 540

Query: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600
           YRRCRSKDKSIGPLEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAIRILDSR 
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRA 600

Query: 601 VGRFFSRKTPFWEPFRVDCSK 621
           VGRFFSRKTPFWEPFR   S+
Sbjct: 601 VGRFFSRKTPFWEPFRAQYSQ 605

BLAST of MC05g0920 vs. ExPASy TrEMBL
Match: A0A5A7TMG2 (Indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002300 PE=3 SV=1)

HSP 1 Score: 1040 bits (2688), Expect = 0.0
Identity = 521/620 (84.03%), Postives = 565/620 (91.13%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           MLPNFDPN+NESGLKLLEDLT NA+QIQEQVI+KIL+QN+NTEYLK+F++N   SHS  D
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIRKILTQNSNTEYLKSFLHNHHYSHSL-D 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           L+ FK+SVPVVNYEDIKPYIERIANGEPS+IIS+QPITELLTSSGTSGGQPKMMPSTAED
Sbjct: 61  LQTFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVP++NK+V GLEEGKGMYLLFIKPE+STPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFN+YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLEDY
Sbjct: 181 RPFNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDY 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           WKE+S NIR G +S+WI+DP+CR+SVSLILTK N  LADLID +CGEKSWEGIIKK+W K
Sbjct: 241 WKELSDNIRKGELSQWISDPNCRASVSLILTKSNPVLADLIDGLCGEKSWEGIIKKLWPK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINF+PLSKP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MAFFEFLPVE+NDG EL     H NG       DE    +TVDLVDV+LG+YYELVVTTF
Sbjct: 361 MAFFEFLPVEKNDG-ELT----HCNGTTNTSVQDEF---KTVDLVDVKLGQYYELVVTTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480
           TGLYRYRVGDILKVTGFHN APQF+FIHRRNVVLSIDTDKTNEDDLL AIT AKLLVEPL
Sbjct: 421 TGLYRYRVGDILKVTGFHNKAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPL 480

Query: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540
           G+LLTEY+SYADT SIPGHYVLFWE+K+R G         V++ +MEECCSSIEESLDSV
Sbjct: 481 GVLLTEYTSYADTESIPGHYVLFWEMKRRSGSNYENFEELVEVDLMEECCSSIEESLDSV 540

Query: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600
           YRRCRSKDKSIGPLEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAI+ILDSRV
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRV 600

Query: 601 VGRFFSRKTPFWEPFRVDCS 620
           V R+FS+KTPFWEPFR   S
Sbjct: 601 VARYFSKKTPFWEPFRGQYS 611

BLAST of MC05g0920 vs. ExPASy TrEMBL
Match: A0A1S3BYQ7 (indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo OX=3656 GN=LOC103494645 PE=3 SV=1)

HSP 1 Score: 1040 bits (2688), Expect = 0.0
Identity = 521/620 (84.03%), Postives = 565/620 (91.13%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           MLPNFDPN+NESGLKLLEDLT NA+QIQEQVI+KIL+QN+NTEYLK+F++N   SHS  D
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIRKILTQNSNTEYLKSFLHNHHYSHSL-D 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
           L+ FK+SVPVVNYEDIKPYIERIANGEPS+IIS+QPITELLTSSGTSGGQPKMMPSTAED
Sbjct: 61  LQTFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAED 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           LDRKTFFYNLLVP++NK+V GLEEGKGMYLLFIKPE+STPSGLMARPVLTSYYKSKNFRN
Sbjct: 121 LDRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFN+YNVYTSPDETILCSDSKQSMYCQLLCGL+QRDEVLRVGAVFASAFLRAIKFLEDY
Sbjct: 181 RPFNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDY 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           WKE+S NIR G +S+WI+DP+CR+SVSLILTK N  LADLID +CGEKSWEGIIKK+W K
Sbjct: 241 WKELSDNIRKGELSQWISDPNCRASVSLILTKSNPVLADLIDGLCGEKSWEGIIKKLWPK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
           TKYIEVIVTGSMAQYIPTLEFYSGGLPL+STMYASSECYFGINF+PLSKP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MAFFEFLPVE+NDG EL     H NG       DE    +TVDLVDV+LG+YYELVVTTF
Sbjct: 361 MAFFEFLPVEKNDG-ELT----HCNGTTNTSVQDEF---KTVDLVDVKLGQYYELVVTTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480
           TGLYRYRVGDILKVTGFHN APQF+FIHRRNVVLSIDTDKTNEDDLL AIT AKLLVEPL
Sbjct: 421 TGLYRYRVGDILKVTGFHNKAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPL 480

Query: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540
           G+LLTEY+SYADT SIPGHYVLFWE+K+R G         V++ +MEECCSSIEESLDSV
Sbjct: 481 GVLLTEYTSYADTESIPGHYVLFWEMKRRSGSNYENFEELVEVDLMEECCSSIEESLDSV 540

Query: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600
           YRRCRSKDKSIGPLEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAI+ILDSRV
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRV 600

Query: 601 VGRFFSRKTPFWEPFRVDCS 620
           V R+FS+KTPFWEPFR   S
Sbjct: 601 VARYFSKKTPFWEPFRGQYS 611

BLAST of MC05g0920 vs. TAIR 10
Match: AT1G28130.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 887.9 bits (2293), Expect = 4.8e-258
Identity = 434/620 (70.00%), Postives = 517/620 (83.39%), Query Frame = 0

Query: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60
           M+P++DPND E+GLKLLEDLT NA  IQ+QV+ +ILSQN+ T+YL+ F++      +  +
Sbjct: 1   MIPSYDPNDTEAGLKLLEDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLD----GEADKN 60

Query: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120
            + FKN VPVVNY+D+KP+I+RIA+GE S+I+SAQPITELLTSSGTS G+PK+MPSTAE+
Sbjct: 61  QQSFKNKVPVVNYDDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEE 120

Query: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180
           L+RKTFFY++LVPIMNK+V GL+EGKGMYLLFIKPEI TPSGLMARPVLTSYYKS++FRN
Sbjct: 121 LERKTFFYSMLVPIMNKYVDGLDEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRN 180

Query: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240
           RPFN+YNVYTSPD+TILC DSKQSMYCQLLCGL+QR  VLRVGAVFASAFLRA+KFLED+
Sbjct: 181 RPFNKYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDH 240

Query: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300
           +KE+  +IR+G V+ WITD SCR SV  IL  PN+ELAD I++ C EKSWEGI+++IW K
Sbjct: 241 YKELCADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAEKSWEGILRRIWPK 300

Query: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360
            KY+EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGIN +PL  P DVSYTLLPN
Sbjct: 301 AKYVEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSYTLLPN 360

Query: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420
           MA+FEFLPV+     E+     H N     DD D + +   V+LV+VE+G+YYE+V+TTF
Sbjct: 361 MAYFEFLPVDDKSHEEI-HFATHSN---TDDDDDALKEDLIVNLVNVEVGQYYEIVITTF 420

Query: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKL--LVE 480
           TGLYRYRVGDILKVTGFHN APQFRF+ RRNVVLSIDTDKT+E+DLL A+T AKL  L  
Sbjct: 421 TGLYRYRVGDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQH 480

Query: 481 PLGILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLD 540
           P  +LLTEY+SYADTSSIPGHYVLFWELK R     +     +D   ME+CCS +E+ LD
Sbjct: 481 PSSLLLTEYTSYADTSSIPGHYVLFWELKPR----HSNDPPKLDDKTMEDCCSEVEDCLD 540

Query: 541 SVYRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDS 600
            VYRRCR++DKSIGPLEIRVV  G FD+LMD+CVSQGSS+NQYKTPRC+KS  A+ ILDS
Sbjct: 541 YVYRRCRNRDKSIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDS 600

Query: 601 RVVGRFFSRKTPFWEPFRVD 619
           RV+GRFFS++ P WEP  +D
Sbjct: 601 RVIGRFFSKRVPQWEPLGLD 608

BLAST of MC05g0920 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 701.8 bits (1810), Expect = 4.8e-202
Identity = 351/605 (58.02%), Postives = 448/605 (74.05%), Query Frame = 0

Query: 10  NESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPDLKLFKNSVP 69
           N++ L+ +ED+T NA  +Q +V+++ILS+NA+ EYLK     G       D + FK+ +P
Sbjct: 21  NKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEG-----RTDRETFKHIMP 80

Query: 70  VVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYN 129
           VV YEDI+P I RIANG+ S ++ + PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+
Sbjct: 81  VVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 140

Query: 130 LLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVY 189
           LL+P+M++ V GL++GKGMY LFIK E  TP GL ARPVLTSYYKS +F+NRP++ Y  Y
Sbjct: 141 LLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNY 200

Query: 190 TSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDYWKEMSHNIR 249
           TSP++TILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W E++ +IR
Sbjct: 201 TSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIR 260

Query: 250 SGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAKTKYIEVIVT 309
           +G +S  ITD S R +V  IL KP+ +LAD +++ C + SW+GII ++W  TKY++VIVT
Sbjct: 261 TGTLSSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVT 320

Query: 310 GSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPNMAFFEFLPV 369
           G+M+QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+PNMA+FEFLPV
Sbjct: 321 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPV 380

Query: 370 ERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTFTGLYRYRVG 429
            RN G   + S P            E  + E VDLVDV+LG+ YELVVTT+ GLYRYRVG
Sbjct: 381 HRNSGVTSSISLPKAL--------TEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVG 440

Query: 430 DILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPLGILLTEYSS 489
           D+L V GF NNAPQF FI R+NVVLSID+DKT+E +L  A+ NA   + P    L+EY+S
Sbjct: 441 DVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTS 500

Query: 490 YADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSVYRRCRSKDK 549
           YADTSSIPGHYVLFWEL   G        T +   V E+CC +IEESL+SVYR+ R  DK
Sbjct: 501 YADTSSIPGHYVLFWELCLNGN-------TPIPPSVFEDCCLTIEESLNSVYRQGRVSDK 560

Query: 550 SIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRVVGRFFSRKT 609
           SIGPLEI++V+ G FD LMDY +S G+S+NQYKTPRC+K    I +L+SRVV  +FS K 
Sbjct: 561 SIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKC 604

Query: 610 PFWEP 615
           P W P
Sbjct: 621 PKWSP 604

BLAST of MC05g0920 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 700.7 bits (1807), Expect = 1.1e-201
Identity = 351/605 (58.02%), Postives = 450/605 (74.38%), Query Frame = 0

Query: 10  NESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPDLKLFKNSVP 69
           N+  L+L+E+LT NA Q+Q QV+++IL++NA+ EYL+    NG       D + FKN +P
Sbjct: 21  NKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNG-----RTDRETFKNIMP 80

Query: 70  VVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYN 129
           V+ YEDI+P I RIANG+ S I+S++PI+E LTSSGTSGG+ K+MP+  E+LDR++  Y+
Sbjct: 81  VITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 140

Query: 130 LLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVY 189
           LL+P+M++ V GLE GKGMY LFIK E  TP GL ARPVLTSYYKS +F+ RP++ Y  Y
Sbjct: 141 LLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNY 200

Query: 190 TSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDYWKEMSHNIR 249
           TSP+ETILCSDS QSMY Q+LCGL Q  EVLRVGAVFAS F+RAIKFLE +W E+  +IR
Sbjct: 201 TSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIR 260

Query: 250 SGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAKTKYIEVIVT 309
           +G +S  ITDPS R +V+ IL KP+ +LAD ++  C + SW+GII ++W  TKY++VIVT
Sbjct: 261 TGTLSSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVT 320

Query: 310 GSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPNMAFFEFLPV 369
           G+M+QYIPTL++YS GLPLV TMYASSECYFG+N  PL KP +VSYTL+P+MA+FEFLPV
Sbjct: 321 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPV 380

Query: 370 ERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTFTGLYRYRVG 429
            RN+G   + + P            E  + E VDLVDV+LG+ YELVVTT+ GL RYRVG
Sbjct: 381 HRNNGVTNSINLPKAL--------TEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVG 440

Query: 430 DILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPLGILLTEYSS 489
           D+L+VTGF N APQF FI R+NVVLSID+DKT+E +L  A+ NA   + P    L+EY+S
Sbjct: 441 DLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTS 500

Query: 490 YADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSVYRRCRSKDK 549
           YADTSSIPGHYVLFWEL   G        T +   V E+CC ++EES ++VYR+ R  DK
Sbjct: 501 YADTSSIPGHYVLFWELCLDGN-------TPIPPSVFEDCCLAVEESFNTVYRQGRVSDK 560

Query: 550 SIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRVVGRFFSRKT 609
           SIGPLEI++V+ G FD LMDY +S G+S+NQYKTPRC+K    I +L+SRVV  +FS K 
Sbjct: 561 SIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKC 604

Query: 610 PFWEP 615
           P W P
Sbjct: 621 PKWVP 604

BLAST of MC05g0920 vs. TAIR 10
Match: AT1G28130.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 691.0 bits (1782), Expect = 8.6e-199
Identity = 338/473 (71.46%), Postives = 391/473 (82.66%), Query Frame = 0

Query: 148 MYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPDETILCSDSKQSMYC 207
           MYLLFIKPEI TPSGLMARPVLTSYYKS++FRNRPFN+YNVYTSPD+TILC DSKQSMYC
Sbjct: 1   MYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFNKYNVYTSPDQTILCQDSKQSMYC 60

Query: 208 QLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDYWKEMSHNIRSGCVSEWITDPSCRSSVS 267
           QLLCGL+QR  VLRVGAVFASAFLRA+KFLED++KE+  +IR+G V+ WITD SCR SV 
Sbjct: 61  QLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKELCADIRTGTVTSWITDSSCRDSVL 120

Query: 268 LILTKPNEELADLIDAMCGEKSWEGIIKKIWAKTKYIEVIVTGSMAQYIPTLEFYSGGLP 327
            IL  PN+ELAD I++ C EKSWEGI+++IW K KY+EVIVTGSMAQYIPTLEFYSGGLP
Sbjct: 121 SILNGPNQELADEIESECAEKSWEGILRRIWPKAKYVEVIVTGSMAQYIPTLEFYSGGLP 180

Query: 328 LVSTMYASSECYFGINFSPLSKPEDVSYTLLPNMAFFEFLPVERNDGGELARSGPHRNGG 387
           LVSTMYASSECYFGIN +PL  P DVSYTLLPNMA+FEFLPV+     E+     H N  
Sbjct: 181 LVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYFEFLPVDDKSHEEI-HFATHSN-- 240

Query: 388 AVRDDGDEMGKLETVDLVDVELGRYYELVVTTFTGLYRYRVGDILKVTGFHNNAPQFRFI 447
              DD D + +   V+LV+VE+G+YYE+V+TTFTGLYRYRVGDILKVTGFHN APQFRF+
Sbjct: 241 -TDDDDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRVGDILKVTGFHNKAPQFRFV 300

Query: 448 HRRNVVLSIDTDKTNEDDLLKAITNAKL--LVEPLGILLTEYSSYADTSSIPGHYVLFWE 507
            RRNVVLSIDTDKT+E+DLL A+T AKL  L  P  +LLTEY+SYADTSSIPGHYVLFWE
Sbjct: 301 QRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTEYTSYADTSSIPGHYVLFWE 360

Query: 508 LKKRGGGGENAAATAVDLGVMEECCSSIEESLDSVYRRCRSKDKSIGPLEIRVVKGGAFD 567
           LK R     +     +D   ME+CCS +E+ LD VYRRCR++DKSIGPLEIRVV  G FD
Sbjct: 361 LKPR----HSNDPPKLDDKTMEDCCSEVEDCLDYVYRRCRNRDKSIGPLEIRVVSLGTFD 420

Query: 568 ALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRVVGRFFSRKTPFWEPFRVD 619
           +LMD+CVSQGSS+NQYKTPRC+KS  A+ ILDSRV+GRFFS++ P WEP  +D
Sbjct: 421 SLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRVPQWEPLGLD 465

BLAST of MC05g0920 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 688.7 bits (1776), Expect = 4.2e-198
Identity = 336/603 (55.72%), Postives = 443/603 (73.47%), Query Frame = 0

Query: 14  LKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPDLKLFKNSVPVVNY 73
           LK +E++T+N   +QE+V+ +IL++N+NTEYLK F  +G       D K FK+ VPVV Y
Sbjct: 24  LKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVV-----DRKTFKSKVPVVTY 83

Query: 74  EDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRKTFFYNLLVP 133
           ED+KP I+RI+NG+ S I+S+ PITE LTSSGTS G+ K+MP+  EDLDR+   Y+LL+P
Sbjct: 84  EDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLMP 143

Query: 134 IMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFNRYNVYTSPD 193
           +MN +V GL++GKG+Y LF+K E  T  GL ARPVLTSYYKS +F+ RP++ YNVYTSP+
Sbjct: 144 VMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSPN 203

Query: 194 ETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDYWKEMSHNIRSGCV 253
           E ILCSDS QSMY Q+LCGL+ R EVLR+GAVFAS  LRAI FL++ WKE++ +I +G +
Sbjct: 204 EAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGTL 263

Query: 254 SEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAKTKYIEVIVTGSMA 313
           S  I DP+ ++ +S ILTKP++ELA+ +  +C +++WEGII KIW  TKY++VIVTG+MA
Sbjct: 264 SSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAMA 323

Query: 314 QYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPNMAFFEFLPVERND 373
           QYIPTLE+YSGGLP+  TMYASSE YFGIN  P+ KP +VSYT++PNMA+FEFLP     
Sbjct: 324 QYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHN--- 383

Query: 374 GGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTFTGLYRYRVGDILK 433
                    H   GA     DE      V+L +VE+G+ YELV+TT+ GLYRYRVGDIL+
Sbjct: 384 ---------HDGDGAAEASLDE---TSLVELANVEVGKEYELVITTYAGLYRYRVGDILR 443

Query: 434 VTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPLGILLTEYSSYADT 493
           VTGFHN+APQF+FI R+NV+LS+++DKT+E +L KA+ NA  L    G  + EY+SYA+T
Sbjct: 444 VTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAET 503

Query: 494 SSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSVYRRCRSKDKSIGP 553
            +IPGHYV++WEL      G + +   +   VM +CC  +EESL+SVYR+ R  DKSIGP
Sbjct: 504 KTIPGHYVIYWELL-----GRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGP 563

Query: 554 LEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRVVGRFFSRKTPFWE 613
           LEIRVV+ G F+ LMDY +S+G+S+NQYK PRC+     + +LDSRVV   FS   P W 
Sbjct: 564 LEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWS 601

Query: 614 PFR 617
           P R
Sbjct: 624 PER 601

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZ876.7e-25770.00Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LSQ46.8e-20158.02Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
O818291.5e-20058.02Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
P0C0M32.1e-19456.02Probable indole-3-acetic acid-amido synthetase GH3.11 OS=Oryza sativa subsp. jap... [more]
O822431.4e-19355.57Putative indole-3-acetic acid-amido synthetase GH3.9 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
XP_022153513.10.0100.00indole-3-acetic acid-amido synthetase GH3.17-like [Momordica charantia][more]
KAG6581972.10.085.35Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023528388.10.085.19indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita pepo subsp. pepo][more]
XP_022980842.10.085.19indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita maxima][more]
XP_022955484.10.085.02indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1DHN60.0100.00indole-3-acetic acid-amido synthetase GH3.17-like OS=Momordica charantia OX=3673... [more]
A0A6J1ISD70.085.19indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1GWE70.085.02indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A5A7TMG20.084.03Indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo var. makuwa OX... [more]
A0A1S3BYQ70.084.03indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo OX=3656 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT1G28130.14.8e-25870.00Auxin-responsive GH3 family protein [more]
AT5G54510.14.8e-20258.02Auxin-responsive GH3 family protein [more]
AT4G27260.11.1e-20158.02Auxin-responsive GH3 family protein [more]
AT1G28130.28.6e-19971.46Auxin-responsive GH3 family protein [more]
AT4G37390.14.2e-19855.72Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 10..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 375..394
NoneNo IPR availablePANTHERPTHR31901:SF33INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.17coord: 3..612
IPR004993GH3 familyPFAMPF03321GH3coord: 17..590
e-value: 6.2E-203
score: 675.4
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 3..612

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0920.1MC05g0920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity