MC05g0825 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0825
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	WRKY domain-containing protein
Location	MC05: 7126538 .. 7129119 (-)
RNA-Seq Expression	MC05g0825
Synteny	MC05g0825

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCAATCTCTCTTAATTTGCTGACGTGTCATGTTATAATTTATAATATATCTTCCTCGTAGCCACAACACAATTAACACATTTTTTGGCATAAAATAAAAATAAATAAATAGAAGGAAAAAAATAAGAAGAAGAAGCTTCCCTTTATAAATGATATCACCTTATTACTTGTCTTTCACGAATATTCAAAGTTCAAACACAAGTATTATTGAAGAACCTCTCATTTCTAGCCCGCCATTTCTCTCTCTCTTCTGCACCAAACGAATCAATGATGGAACAATATTTCCCATCAACTTCCCTCTCGCTGCACCAGATGCTGATTTCTCGAGCTCCCGAGCTCGCCGGGATCCATGGAGCTGGCCCGTTACAGCGCCCGCACGCCTCCGGTGGGTTTCCGGTGTTTCGCGATGTTTCTATCGACGACATGTCGGTCGATGGAGTGAGGAGTATGAAGGATGACGTCGTTTCGCTGAGTAGAAGCGTCCGTTTTGTTGATGGGTCAGCCGGGGAGGGAGTGGAAGAGCTGGAGAAGAAAGCGGCGGGGGCCGGGACGAGTACAAGGACGACCGGGCCGGAGGCAGGGCGGGGAGATTTTGAGAAGAAGAAGAGGAGGCAGAGATATGCCTTTCAAACCAGGAGTCAAGTTGATATTCTTGATGATGGTTATAGATGGAGAAAATATGGACAAAAGGCTGTGAAGAACAACAAATTCCCAAGGTAATTCAATTCTACTTTCTTGTTGGTTTTTCTTTTTCTTTTTTTTTTTAACTTTTATATATAATAGTGCATGAGATTCAAACCTTATTCGATTCATATTGAAGGTTGTTTGTTCGTGGATTTTTTTTCCTGCAAAATTTGCATAAAAAAATTAGAGTAATAATTTGAATCTACTCTATTTTAAGAGAAGTTTATAGAGTAATTTAGGAATTAATAGTAATTTAGTTGGCATTTTTTTTCTTTTTTTTTTGCAAGCAATTTAGTTGCCATATCGGTGGTAATTGAATTTTTAGGAATTAATAGAGGAGGGTTGATGTGTCATTAAATTTTGTTCATATATTTGGATATGTAGGTATGAGAAATCCATTTCAAGCTAGTCAAATTTTATTGATTATGCCAATTGTCATTTTTGTATGTCAAATAGGATTGGAGTTATATTCATGTGTTGAAATAAAATTAAACAATAAGTTAATAATACATCAACTTTTTTAAATAAATTTTAATAGTTCTTTTTTAGGGAATAAGCTAATTGAAAACTTGAAAACTTTTTATGTATTAAGGGCAAGGGTAAAATTGAGAATCCTAAAAATCAATAAACATTTGGTAGCGTTACCACTCATAAATCTTTTTAGGTTGAAATCTTTGTTAATATTGGATAACAATTTTTGTACCTTTTAACATTGGATAGATATGATAATTATGTTCTTAGCGTTAAAAGATGCACTTAAAAAGTCAATGGTATTAATTATTTTAAAGTTAAATATAATTTTTCTTCTCTATAATTTAAGTGCTTCAATTTGTTGATATGATTTTATAAACCAACAATGCCTACTCATGCTGTTATGATAAGGTAACCATATTATCTTAAAATCGCTTTCAAATTCTAAGTCAAACTAAAAATAAAAAATAAAAAAAAATTCAAAATATTAATTTTGTTTTTTAAGTTTGATTAAGAATTCATATTTGTTATAAAGAAAATGAAAAACATACTAAATAAATAATGAAAACATAAGTATAACTTAAAAAAAAAAAAAAAACGAAATCATTGTCAAACCAAATCAACAATCCTAAAGATAGAAAAATAAAATAAAACAAAACTCATTTGATAACAATTAATCAATATTAGATTGATTTTTTTTAGTACAACTAGTGGGAGCGGGAGATTCGAATTCATGACTTTGACCGGTGACATTTACCGTTGAGCTAATCTCATATTGACTCAATACCTAGATTAATTAGTCATATATGTCATATATCTTTTTATAGTTGATTACTAGTAACAACCAATTAATTAGCACATACGCCTTCCGTAAGATCAAAGATTCAAATCCACATATCCTAAATATATAAAGATGACTAGAATATAATGTATGAGTGAAGGAACCTTCAATAGCGGGAAATGTATATGTGTGTTAACAACTAAACTATATACTCGAGTTCAGAGTAATTAATTAAACCCTATATTCAATACTTAACTAATAATAAACTAGCTAGCGTAGATAATGAAGTTAATAATGATTAGGGTTGATTGAATTATTTGGGTGAAATAATTATGTTGATAACATTTTGTTTGGGTGATGAATTGAAAAATTGCAGAAGCTACTATAGATGTACACACCAAGGTTGCAAAGTGAAGAAGCAAGTGCAAAGACTGACTAAAGATGAAGCTGTGGTTATGACCACTTATGAAGGCATTCACTCTCATCCCATTGACAAATCCACTGATAACTTTGAGCATATTTTGACTCAGATGCAAATTTACACTTCTCATTGATCAATTTTTTTTCCCCACATACAACTTGATATTATTCATCATCATTGTAAAGTTCATTACAATTTTTTCTATAAGAAATCTAAACATTTTTC

mRNA sequence

GCCAATCTCTCTTAATTTGCTGACGTGTCATGTTATAATTTATAATATATCTTCCTCGTAGCCACAACACAATTAACACATTTTTTGGCATAAAATAAAAATAAATAAATAGAAGGAAAAAAATAAGAAGAAGAAGCTTCCCTTTATAAATGATATCACCTTATTACTTGTCTTTCACGAATATTCAAAGTTCAAACACAAGTATTATTGAAGAACCTCTCATTTCTAGCCCGCCATTTCTCTCTCTCTTCTGCACCAAACGAATCAATGATGGAACAATATTTCCCATCAACTTCCCTCTCGCTGCACCAGATGCTGATTTCTCGAGCTCCCGAGCTCGCCGGGATCCATGGAGCTGGCCCGTTACAGCGCCCGCACGCCTCCGGTGGGTTTCCGGTGTTTCGCGATGTTTCTATCGACGACATGTCGGTCGATGGAGTGAGGAGTATGAAGGATGACGTCGTTTCGCTGAGTAGAAGCGTCCGTTTTGTTGATGGGTCAGCCGGGGAGGGAGTGGAAGAGCTGGAGAAGAAAGCGGCGGGGGCCGGGACGAGTACAAGGACGACCGGGCCGGAGGCAGGGCGGGGAGATTTTGAGAAGAAGAAGAGGAGGCAGAGATATGCCTTTCAAACCAGGAGTCAAGTTGATATTCTTGATGATGGTTATAGATGGAGAAAATATGGACAAAAGGCTGTGAAGAACAACAAATTCCCAAGAAGCTACTATAGATGTACACACCAAGGTTGCAAAGTGAAGAAGCAAGTGCAAAGACTGACTAAAGATGAAGCTGTGGTTATGACCACTTATGAAGGCATTCACTCTCATCCCATTGACAAATCCACTGATAACTTTGAGCATATTTTGACTCAGATGCAAATTTACACTTCTCATTGATCAATTTTTTTTCCCCACATACAACTTGATATTATTCATCATCATTGTAAAGTTCATTACAATTTTTTCTATAAGAAATCTAAACATTTTTC

Coding sequence (CDS)

ATGATGGAACAATATTTCCCATCAACTTCCCTCTCGCTGCACCAGATGCTGATTTCTCGAGCTCCCGAGCTCGCCGGGATCCATGGAGCTGGCCCGTTACAGCGCCCGCACGCCTCCGGTGGGTTTCCGGTGTTTCGCGATGTTTCTATCGACGACATGTCGGTCGATGGAGTGAGGAGTATGAAGGATGACGTCGTTTCGCTGAGTAGAAGCGTCCGTTTTGTTGATGGGTCAGCCGGGGAGGGAGTGGAAGAGCTGGAGAAGAAAGCGGCGGGGGCCGGGACGAGTACAAGGACGACCGGGCCGGAGGCAGGGCGGGGAGATTTTGAGAAGAAGAAGAGGAGGCAGAGATATGCCTTTCAAACCAGGAGTCAAGTTGATATTCTTGATGATGGTTATAGATGGAGAAAATATGGACAAAAGGCTGTGAAGAACAACAAATTCCCAAGAAGCTACTATAGATGTACACACCAAGGTTGCAAAGTGAAGAAGCAAGTGCAAAGACTGACTAAAGATGAAGCTGTGGTTATGACCACTTATGAAGGCATTCACTCTCATCCCATTGACAAATCCACTGATAACTTTGAGCATATTTTGACTCAGATGCAAATTTACACTTCTCATTGA

Protein sequence

MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRSMKDDVVSLSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQIYTSH

Homology

[Close]

BLAST of MC05g0825 vs. ExPASy Swiss-Prot
Match: Q9FYA2 (Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 8.8e-44
Identity = 85/113 (75.22%), Postives = 97/113 (85.84%), Query Frame = 0


Query: 95  TSTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYR 154
           +  R+ G        +KK ++QRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYR
Sbjct: 32  SKVRSEGCSKSVESSKKKGKKQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYR 91

Query: 155 CTHQGCKVKKQVQRLTKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQIYTS 208
           CT+ GC VKKQVQRLT D+ VV+TTYEG+HSHPI+KST+NFEHILTQMQIY+S
Sbjct: 92  CTYGGCNVKKQVQRLTVDQEVVVTTYEGVHSHPIEKSTENFEHILTQMQIYSS 144

[Close]

BLAST of MC05g0825 vs. ExPASy Swiss-Prot
Match: Q9S763 (Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.5e-39
Identity = 75/107 (70.09%), Postives = 88/107 (82.24%), Query Frame = 0


Query: 99  TTGPEAGRGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQ 158
           T+  E  R   +KK+R  RYAFQTRSQVDILDDGYRWRKYGQKAVKNN FPRSYY+CT +
Sbjct: 34  TSSEEKPRSKKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCTEE 93

Query: 159 GCKVKKQVQRLTKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQIY 206
           GC+VKKQVQR   DE VV+TTY+G+H+H +DK +DNF HILTQM I+
Sbjct: 94  GCRVKKQVQRQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIF 140

[Close]

BLAST of MC05g0825 vs. ExPASy Swiss-Prot
Match: Q9FFS3 (Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.3e-28
Identity = 62/106 (58.49%), Postives = 77/106 (72.64%), Query Frame = 0


Query: 103 EAGRGDFEKKKRR-QRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCK 162
           E G+   EK+ R+  R AF TRS  D+LDDGYRWRKYGQK+VK+N  PRSYYRCT+  C 
Sbjct: 70  EKGKELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCN 129

Query: 163 VKKQVQRLTKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQIYTS 208
           VKKQVQRL KD  VV+TTYEG+H+HP +K  +    +L Q+Q  +S
Sbjct: 130 VKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLSS 175

[Close]

BLAST of MC05g0825 vs. ExPASy Swiss-Prot
Match: Q8VWQ4 (Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.3e-28
Identity = 59/98 (60.20%), Postives = 72/98 (73.47%), Query Frame = 0


Query: 106 RGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQ 165
           +G  ++    QR AF TRS  D+LDDGYRWRKYGQK+VKNN  PRSYYRCT+  C VKKQ
Sbjct: 90  KGKGKRTLAMQRIAFHTRSDDDVLDDGYRWRKYGQKSVKNNAHPRSYYRCTYHTCNVKKQ 149

Query: 166 VQRLTKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQ 204
           VQRL KD  VV+TTYEG+H+HP +K  +    +L Q+Q
Sbjct: 150 VQRLAKDPNVVVTTYEGVHNHPCEKLMETLSPLLRQLQ 187

[Close]

BLAST of MC05g0825 vs. ExPASy Swiss-Prot
Match: Q8GY11 (Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.5e-27
Identity = 55/94 (58.51%), Postives = 73/94 (77.66%), Query Frame = 0


Query: 110 EKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRL 169
           +KK +  R++F+T+S  DILDDGYRWRKYGQK+VKN+ +PRSYYRCT   C VKKQVQRL
Sbjct: 10  DKKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQRL 69

Query: 170 TKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQ 204
           +K+ ++V TTYEGIH+HP ++       +L Q+Q
Sbjct: 70  SKETSIVETTYEGIHNHPCEELMQTLTPLLHQLQ 103

[Close]

BLAST of MC05g0825 vs. NCBI nr
Match: XP_022145186.1 (probable WRKY transcription factor 75 [Momordica charantia])

HSP 1 Score: 414 bits (1065), Expect = 5.44e-146
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS 60
           MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS
Sbjct: 1   MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS 60

Query: 61  MKDDVVSLSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAF 120
           MKDDVVSLSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAF
Sbjct: 61  MKDDVVSLSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAF 120

Query: 121 QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTY 180
           QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTY
Sbjct: 121 QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTY 180

Query: 181 EGIHSHPIDKSTDNFEHILTQMQIYTSH 208
           EGIHSHPIDKSTDNFEHILTQMQIYTSH
Sbjct: 181 EGIHSHPIDKSTDNFEHILTQMQIYTSH 208

[Close]

BLAST of MC05g0825 vs. NCBI nr
Match: XP_038902977.1 (probable WRKY transcription factor 50 [Benincasa hispida])

HSP 1 Score: 268 bits (685), Expect = 5.69e-88
Identity = 149/206 (72.33%), Postives = 163/206 (79.13%), Query Frame = 0


Query: 8   STSLSLHQMLISRAPELAGIHGAGPL-QRPHASGGFPVFRDVSIDDMSVDGVRSMKDD-- 67
           S+SLS HQML SRA EL G HG     Q P  SGGFP+ RDV  DDMS D   SMKDD  
Sbjct: 15  SSSLSFHQMLFSRASELVGSHGGDHYRQPPPVSGGFPLSRDVLGDDMSFDASSSMKDDDV 74

Query: 68  VVSLSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKK--RRQRYAFQT 127
           +VSLS+S R VD SA  GV+ LEKK      +T TTG   GRGD +KKK  R +R+AFQT
Sbjct: 75  IVSLSKSFR-VDESARIGVDGLEKKEVATAGATTTTG--TGRGDHDKKKKMRSRRFAFQT 134

Query: 128 RSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTYEG 187
           RSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLT+DE VV+TTYEG
Sbjct: 135 RSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEG 194

Query: 188 IHSHPIDKSTDNFEHILTQMQIYTSH 208
           IHSHPI+KSTDNFEHIL+QMQIYTS+
Sbjct: 195 IHSHPIEKSTDNFEHILSQMQIYTSY 217

[Close]

BLAST of MC05g0825 vs. NCBI nr
Match: XP_022972157.1 (probable WRKY transcription factor 75 [Cucurbita maxima])

HSP 1 Score: 242 bits (617), Expect = 8.56e-78
Identity = 133/201 (66.17%), Postives = 149/201 (74.13%), Query Frame = 0


Query: 8   STSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRSMKDDVVS 67
           S SLS  + LISRA E  GIHG+   +    SGGFP+F DVS   MSVD   SMK+D+VS
Sbjct: 15  SNSLSFQKTLISRASEPTGIHGSH-FREAGISGGFPLFPDVSRSYMSVDTRSSMKEDMVS 74

Query: 68  LSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVD 127
           +S+     DGSAGE VE       G   +T       G  D +KK R +R+AFQTRSQVD
Sbjct: 75  VSKGFH-ADGSAGEVVE-------GGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVD 134

Query: 128 ILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTYEGIHSHP 187
           ILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE VV+TTYEGIHSHP
Sbjct: 135 ILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHP 194

Query: 188 IDKSTDNFEHILTQMQIYTSH 208
           I KSTDNFEHILTQMQIYTS+
Sbjct: 195 IQKSTDNFEHILTQMQIYTSY 206

[Close]

BLAST of MC05g0825 vs. NCBI nr
Match: XP_022932710.1 (probable WRKY transcription factor 75 [Cucurbita moschata])

HSP 1 Score: 238 bits (606), Expect = 3.40e-76
Identity = 133/201 (66.17%), Postives = 149/201 (74.13%), Query Frame = 0


Query: 8   STSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRSMKDDVVS 67
           STSLS  +MLISRA E  GIHG+   +    SGGFP+F DVS   MSV    SMK+D+VS
Sbjct: 15  STSLSFQKMLISRASEPTGIHGSH-FREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVS 74

Query: 68  LSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVD 127
           +S+      GSA E VE       G   +T  T    G  D +KK R +R+AFQTRSQVD
Sbjct: 75  VSK------GSAVEVVE-------GGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQVD 134

Query: 128 ILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTYEGIHSHP 187
           ILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE VV+TTYEGIHSHP
Sbjct: 135 ILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHP 194

Query: 188 IDKSTDNFEHILTQMQIYTSH 208
           I KSTDNFEHILTQMQIYTS+
Sbjct: 195 IQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of MC05g0825 vs. NCBI nr
Match: XP_023539084.1 (probable WRKY transcription factor 75 isoform X1 [Cucurbita pepo subsp. pepo] >AXF54192.1 WRKY1 [Cucurbita pepo])

HSP 1 Score: 236 bits (601), Expect = 1.96e-75
Identity = 132/201 (65.67%), Postives = 148/201 (73.63%), Query Frame = 0


Query: 8   STSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRSMKDDVVS 67
           STSLS  +MLISRA E  GIHG+   +    SGGFP+F DVS   MSV    S K+D+VS
Sbjct: 15  STSLSFQKMLISRASEPTGIHGS-QFREAAISGGFPLFPDVSGSYMSVGTRSSTKEDMVS 74

Query: 68  LSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVD 127
           +S+      GSA E VE       G   +T  T    G  D +KK R +R+AFQTRSQVD
Sbjct: 75  VSK------GSAVEVVE-------GGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQVD 134

Query: 128 ILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTYEGIHSHP 187
           ILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE VV+TTYEGIHSHP
Sbjct: 135 ILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHP 194

Query: 188 IDKSTDNFEHILTQMQIYTSH 208
           I KSTDNFEHILTQMQIYTS+
Sbjct: 195 IQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of MC05g0825 vs. ExPASy TrEMBL
Match: A0A6J1CVL3 (probable WRKY transcription factor 75 OS=Momordica charantia OX=3673 GN=LOC111014691 PE=4 SV=1)

HSP 1 Score: 414 bits (1065), Expect = 2.64e-146
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS 60
           MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS
Sbjct: 1   MMEQYFPSTSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRS 60

Query: 61  MKDDVVSLSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAF 120
           MKDDVVSLSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAF
Sbjct: 61  MKDDVVSLSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAF 120

Query: 121 QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTY 180
           QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTY
Sbjct: 121 QTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTY 180

Query: 181 EGIHSHPIDKSTDNFEHILTQMQIYTSH 208
           EGIHSHPIDKSTDNFEHILTQMQIYTSH
Sbjct: 181 EGIHSHPIDKSTDNFEHILTQMQIYTSH 208

[Close]

BLAST of MC05g0825 vs. ExPASy TrEMBL
Match: A0A6J1I419 (probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC111470780 PE=4 SV=1)

HSP 1 Score: 242 bits (617), Expect = 4.14e-78
Identity = 133/201 (66.17%), Postives = 149/201 (74.13%), Query Frame = 0


Query: 8   STSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRSMKDDVVS 67
           S SLS  + LISRA E  GIHG+   +    SGGFP+F DVS   MSVD   SMK+D+VS
Sbjct: 15  SNSLSFQKTLISRASEPTGIHGSH-FREAGISGGFPLFPDVSRSYMSVDTRSSMKEDMVS 74

Query: 68  LSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVD 127
           +S+     DGSAGE VE       G   +T       G  D +KK R +R+AFQTRSQVD
Sbjct: 75  VSKGFH-ADGSAGEVVE-------GGAETTEMAAARQGDNDKKKKTRSRRFAFQTRSQVD 134

Query: 128 ILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTYEGIHSHP 187
           ILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE VV+TTYEGIHSHP
Sbjct: 135 ILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHP 194

Query: 188 IDKSTDNFEHILTQMQIYTSH 208
           I KSTDNFEHILTQMQIYTS+
Sbjct: 195 IQKSTDNFEHILTQMQIYTSY 206

[Close]

BLAST of MC05g0825 vs. ExPASy TrEMBL
Match: A0A6J1F2W9 (probable WRKY transcription factor 75 OS=Cucurbita moschata OX=3662 GN=LOC111439177 PE=4 SV=1)

HSP 1 Score: 238 bits (606), Expect = 1.65e-76
Identity = 133/201 (66.17%), Postives = 149/201 (74.13%), Query Frame = 0


Query: 8   STSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRSMKDDVVS 67
           STSLS  +MLISRA E  GIHG+   +    SGGFP+F DVS   MSV    SMK+D+VS
Sbjct: 15  STSLSFQKMLISRASEPTGIHGSH-FREAGISGGFPLFPDVSGSYMSVGTRSSMKEDMVS 74

Query: 68  LSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVD 127
           +S+      GSA E VE       G   +T  T    G  D +KK R +R+AFQTRSQVD
Sbjct: 75  VSK------GSAVEVVE-------GGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQVD 134

Query: 128 ILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTYEGIHSHP 187
           ILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE VV+TTYEGIHSHP
Sbjct: 135 ILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHP 194

Query: 188 IDKSTDNFEHILTQMQIYTSH 208
           I KSTDNFEHILTQMQIYTS+
Sbjct: 195 IQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of MC05g0825 vs. ExPASy TrEMBL
Match: A0A345BTD5 (WRKY1 OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 236 bits (601), Expect = 9.47e-76
Identity = 132/201 (65.67%), Postives = 148/201 (73.63%), Query Frame = 0


Query: 8   STSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPVFRDVSIDDMSVDGVRSMKDDVVS 67
           STSLS  +MLISRA E  GIHG+   +    SGGFP+F DVS   MSV    S K+D+VS
Sbjct: 15  STSLSFQKMLISRASEPTGIHGS-QFREAAISGGFPLFPDVSGSYMSVGTRSSTKEDMVS 74

Query: 68  LSRSVRFVDGSAGEGVEELEKKAAGAGTSTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVD 127
           +S+      GSA E VE       G   +T  T    G  D +KK R +R+AFQTRSQVD
Sbjct: 75  VSK------GSAVEVVE-------GGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQVD 134

Query: 128 ILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVVMTTYEGIHSHP 187
           ILDDGYRWRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLT+DE VV+TTYEGIHSHP
Sbjct: 135 ILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHP 194

Query: 188 IDKSTDNFEHILTQMQIYTSH 208
           I KSTDNFEHILTQMQIYTS+
Sbjct: 195 IQKSTDNFEHILTQMQIYTSY 201

[Close]

BLAST of MC05g0825 vs. ExPASy TrEMBL
Match: A0A0A0KU50 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G012390 PE=4 SV=1)

HSP 1 Score: 233 bits (593), Expect = 2.47e-74
Identity = 136/211 (64.45%), Postives = 151/211 (71.56%), Query Frame = 0


Query: 8   STSLSLHQMLISRAPELAGIHGAGPLQRPHASGGFPV-FRDVSIDDMSVDGVRSMKDD-- 67
           S++LSL Q+L SRA    G H   P   P  S GFP+ FRDVS DDMS D   S+KDD  
Sbjct: 15  SSALSLQQILFSRASNHGGDHFRQP---PFVSDGFPILFRDVSTDDMSFDATSSVKDDDR 74

Query: 68  ---VVSLSRSVRFVDGSAGEG--VEELEKKAAGAGTSTRTTGPEAGRG---DFEKKKRRQ 127
               VS+S     VDGS+G    VE   KK  G G          GRG   D +KK R +
Sbjct: 75  NNVAVSVSTERFRVDGSSGRSGVVEYGLKKEEGEGR---------GRGEDCDKKKKMRNR 134

Query: 128 RYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTKDEAVV 187
           R+AFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLT+DE VV
Sbjct: 135 RFAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVV 194

Query: 188 MTTYEGIHSHPIDKSTDNFEHILTQMQIYTS 207
           +TTYEGIHSHPI+KSTDNFEHIL+QMQIYT+
Sbjct: 195 VTTYEGIHSHPIEKSTDNFEHILSQMQIYTT 213

[Close]

BLAST of MC05g0825 vs. TAIR 10
Match: AT5G13080.1 (WRKY DNA-binding protein 75 )

HSP 1 Score: 178.3 bits (451), Expect = 6.2e-45
Identity = 85/113 (75.22%), Postives = 97/113 (85.84%), Query Frame = 0


Query: 95  TSTRTTGPEAGRGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYR 154
           +  R+ G        +KK ++QRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYR
Sbjct: 32  SKVRSEGCSKSVESSKKKGKKQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYR 91

Query: 155 CTHQGCKVKKQVQRLTKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQIYTS 208
           CT+ GC VKKQVQRLT D+ VV+TTYEG+HSHPI+KST+NFEHILTQMQIY+S
Sbjct: 92  CTYGGCNVKKQVQRLTVDQEVVVTTYEGVHSHPIEKSTENFEHILTQMQIYSS 144

[Close]

BLAST of MC05g0825 vs. TAIR 10
Match: AT3G01970.1 (WRKY DNA-binding protein 45 )

HSP 1 Score: 161.8 bits (408), Expect = 6.0e-40
Identity = 75/107 (70.09%), Postives = 88/107 (82.24%), Query Frame = 0


Query: 99  TTGPEAGRGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQ 158
           T+  E  R   +KK+R  RYAFQTRSQVDILDDGYRWRKYGQKAVKNN FPRSYY+CT +
Sbjct: 34  TSSEEKPRSKKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCTEE 93

Query: 159 GCKVKKQVQRLTKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQIY 206
           GC+VKKQVQR   DE VV+TTY+G+H+H +DK +DNF HILTQM I+
Sbjct: 94  GCRVKKQVQRQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIF 140

[Close]

BLAST of MC05g0825 vs. TAIR 10
Match: AT1G64000.1 (WRKY DNA-binding protein 56 )

HSP 1 Score: 127.1 bits (318), Expect = 1.7e-29
Identity = 59/98 (60.20%), Postives = 72/98 (73.47%), Query Frame = 0


Query: 106 RGDFEKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQ 165
           +G  ++    QR AF TRS  D+LDDGYRWRKYGQK+VKNN  PRSYYRCT+  C VKKQ
Sbjct: 90  KGKGKRTLAMQRIAFHTRSDDDVLDDGYRWRKYGQKSVKNNAHPRSYYRCTYHTCNVKKQ 149

Query: 166 VQRLTKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQ 204
           VQRL KD  VV+TTYEG+H+HP +K  +    +L Q+Q
Sbjct: 150 VQRLAKDPNVVVTTYEGVHNHPCEKLMETLSPLLRQLQ 187

[Close]

BLAST of MC05g0825 vs. TAIR 10
Match: AT5G41570.1 (WRKY DNA-binding protein 24 )

HSP 1 Score: 127.1 bits (318), Expect = 1.7e-29
Identity = 62/106 (58.49%), Postives = 77/106 (72.64%), Query Frame = 0


Query: 103 EAGRGDFEKKKRR-QRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCK 162
           E G+   EK+ R+  R AF TRS  D+LDDGYRWRKYGQK+VK+N  PRSYYRCT+  C 
Sbjct: 70  EKGKELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCN 129

Query: 163 VKKQVQRLTKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQIYTS 208
           VKKQVQRL KD  VV+TTYEG+H+HP +K  +    +L Q+Q  +S
Sbjct: 130 VKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLSS 175

[Close]

BLAST of MC05g0825 vs. TAIR 10
Match: AT2G46130.1 (WRKY DNA-binding protein 43 )

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-28
Identity = 55/94 (58.51%), Postives = 73/94 (77.66%), Query Frame = 0


Query: 110 EKKKRRQRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRL 169
           +KK +  R++F+T+S  DILDDGYRWRKYGQK+VKN+ +PRSYYRCT   C VKKQVQRL
Sbjct: 10  DKKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQRL 69

Query: 170 TKDEAVVMTTYEGIHSHPIDKSTDNFEHILTQMQ 204
           +K+ ++V TTYEGIH+HP ++       +L Q+Q
Sbjct: 70  SKETSIVETTYEGIHNHPCEELMQTLTPLLHQLQ 103

The following BLAST results are available for this feature:

BLAST of MC05g0825 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYA2	8.8e-44	75.22	Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 ...	[more]
Q9S763	8.5e-39	70.09	Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 ...	[more]
Q9FFS3	2.3e-28	58.49	Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 ...	[more]
Q8VWQ4	2.3e-28	60.20	Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 ...	[more]
Q8GY11	1.5e-27	58.51	Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 ...	[more]

BLAST of MC05g0825 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145186.1	5.44e-146	100.00	probable WRKY transcription factor 75 [Momordica charantia]	[more]
XP_038902977.1	5.69e-88	72.33	probable WRKY transcription factor 50 [Benincasa hispida]	[more]
XP_022972157.1	8.56e-78	66.17	probable WRKY transcription factor 75 [Cucurbita maxima]	[more]
XP_022932710.1	3.40e-76	66.17	probable WRKY transcription factor 75 [Cucurbita moschata]	[more]
XP_023539084.1	1.96e-75	65.67	probable WRKY transcription factor 75 isoform X1 [Cucurbita pepo subsp. pepo] >A...	[more]

BLAST of MC05g0825 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CVL3	2.64e-146	100.00	probable WRKY transcription factor 75 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1I419	4.14e-78	66.17	probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC11147078...	[more]
A0A6J1F2W9	1.65e-76	66.17	probable WRKY transcription factor 75 OS=Cucurbita moschata OX=3662 GN=LOC111439...	[more]
A0A345BTD5	9.47e-76	65.67	WRKY1 OS=Cucurbita pepo OX=3663 PE=2 SV=1	[more]
A0A0A0KU50	2.47e-74	64.45	WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G012390 PE=4 S...	[more]

BLAST of MC05g0825 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G13080.1	6.2e-45	75.22	WRKY DNA-binding protein 75	[more]
AT3G01970.1	6.0e-40	70.09	WRKY DNA-binding protein 45	[more]
AT1G64000.1	1.7e-29	60.20	WRKY DNA-binding protein 56	[more]
AT5G41570.1	1.7e-29	58.49	WRKY DNA-binding protein 24	[more]
AT2G46130.1	1.1e-28	58.51	WRKY DNA-binding protein 43	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 129..188 e-value: 1.2E-38 score: 144.4
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 130..187 e-value: 5.2E-26 score: 90.7
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 124..189 score: 29.527967
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 114..188 e-value: 4.2E-33 score: 115.0
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 121..188
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..107
None	No IPR available	PANTHER	PTHR31221:SF222	WRKY TRANSCRIPTION FACTOR 75-RELATED	coord: 108..205
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 108..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0825.1	MC05g0825.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding