MC05g0793 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0793
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDNA-directed RNA polymerase subunit beta
LocationMC05: 6672331 .. 6672504 (-)
RNA-Seq ExpressionMC05g0793
SyntenyMC05g0793
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTTACACACAACACCCCTTAGAGGAAGGGCCAAGCAAGGTGGACAACGGGTGGGAGAAATAGAGGTTTGGGCTCTAGAGGGATTTGGTGTTGCTCATATTTTGCAAGAGATGCTTACTTATAAATCTGATCATATTAGAGCTCGGCAAGATATACTTGATACTACGATCATC

mRNA sequence

TTGTTACACACAACACCCCTTAGAGGAAGGGCCAAGCAAGGTGGACAACGGGTGGGAGAAATAGAGGTTTGGGCTCTAGAGGGATTTGGTGTTGCTCATATTTTGCAAGAGATGCTTACTTATAAATCTGATCATATTAGAGCTCGGCAAGATATACTTGATACTACGATCATC

Coding sequence (CDS)

TTGTTACACACAACACCCCTTAGAGGAAGGGCCAAGCAAGGTGGACAACGGGTGGGAGAAATAGAGGTTTGGGCTCTAGAGGGATTTGGTGTTGCTCATATTTTGCAAGAGATGCTTACTTATAAATCTGATCATATTAGAGCTCGGCAAGATATACTTGATACTACGATCATC

Protein sequence

LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII
Homology
BLAST of MC05g0793 vs. ExPASy Swiss-Prot
Match: Q589C0 (DNA-directed RNA polymerase subunit beta OS=Silene latifolia OX=37657 GN=rpoB PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.5e-22
Identity = 50/58 (86.21%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1    LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 59
            L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHI+ARQD+L TTII
Sbjct: 968  LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIKARQDVLGTTII 1025

BLAST of MC05g0793 vs. ExPASy Swiss-Prot
Match: A9LYH7 (DNA-directed RNA polymerase subunit beta OS=Acorus americanus OX=263995 GN=rpoB PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 2.0e-22
Identity = 50/58 (86.21%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1    LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 59
            L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTII
Sbjct: 981  LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTII 1038

BLAST of MC05g0793 vs. ExPASy Swiss-Prot
Match: Q3V542 (DNA-directed RNA polymerase subunit beta OS=Acorus calamus OX=4465 GN=rpoB PE=3 SV=2)

HSP 1 Score: 105.5 bits (262), Expect = 2.0e-22
Identity = 50/58 (86.21%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1    LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 59
            L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTII
Sbjct: 969  LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTII 1026

BLAST of MC05g0793 vs. ExPASy Swiss-Prot
Match: Q5QA72 (DNA-directed RNA polymerase subunit beta OS=Acorus gramineus OX=55184 GN=rpoB PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 2.0e-22
Identity = 50/58 (86.21%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1    LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 59
            L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTII
Sbjct: 969  LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTII 1026

BLAST of MC05g0793 vs. ExPASy Swiss-Prot
Match: Q8S8X9 (DNA-directed RNA polymerase subunit beta OS=Atropa belladonna OX=33113 GN=rpoB PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 2.0e-22
Identity = 50/58 (86.21%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1    LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 59
            L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTII
Sbjct: 968  LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTII 1025

BLAST of MC05g0793 vs. NCBI nr
Match: BBN70082.1 (hypothetical protein Prudu_1405S000400 [Prunus dulcis])

HSP 1 Score: 104 bits (260), Expect = 2.76e-27
Identity = 50/58 (86.21%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1  LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 58
          L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTII
Sbjct: 16 LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTII 73

BLAST of MC05g0793 vs. NCBI nr
Match: NLZ73073.1 (DNA-directed RNA polymerase subunit beta [Bacteroidales bacterium])

HSP 1 Score: 104 bits (259), Expect = 5.36e-27
Identity = 49/58 (84.48%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1  LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 58
          L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTI+
Sbjct: 27 LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIV 84

BLAST of MC05g0793 vs. NCBI nr
Match: VVW90808.1 (unnamed protein product, partial [Nymphaea colorata])

HSP 1 Score: 104 bits (259), Expect = 1.73e-26
Identity = 49/58 (84.48%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1   LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 58
           L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTI+
Sbjct: 71  LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIV 128

BLAST of MC05g0793 vs. NCBI nr
Match: WP_199287517.1 (hypothetical protein, partial [Photobacterium chitinilyticum] >RWX52626.1 DNA-directed RNA polymerase subunit beta, partial [Photobacterium chitinilyticum])

HSP 1 Score: 103 bits (257), Expect = 2.21e-26
Identity = 49/58 (84.48%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1   LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 58
           L+   PLRGR+KQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTII
Sbjct: 88  LVTQQPLRGRSKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTII 145

BLAST of MC05g0793 vs. NCBI nr
Match: VVW90363.1 (unnamed protein product, partial [Nymphaea colorata])

HSP 1 Score: 104 bits (259), Expect = 3.19e-26
Identity = 49/58 (84.48%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1   LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 58
           L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTI+
Sbjct: 131 LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIV 188

BLAST of MC05g0793 vs. ExPASy TrEMBL
Match: A0A5H2XTM0 (DNA-directed RNA polymerase OS=Prunus dulcis OX=3755 GN=Prudu_1405S000400 PE=3 SV=1)

HSP 1 Score: 104 bits (260), Expect = 1.34e-27
Identity = 50/58 (86.21%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1  LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 58
          L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTII
Sbjct: 16 LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTII 73

BLAST of MC05g0793 vs. ExPASy TrEMBL
Match: A0A7X8ZSZ9 (DNA-directed RNA polymerase subunit beta OS=Bacteroidales bacterium OX=2030927 GN=GX905_04560 PE=4 SV=1)

HSP 1 Score: 104 bits (259), Expect = 2.59e-27
Identity = 49/58 (84.48%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1  LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 58
          L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTI+
Sbjct: 27 LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIV 84

BLAST of MC05g0793 vs. ExPASy TrEMBL
Match: A0A5K1HTK9 (DNA-directed RNA polymerase (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS30763 PE=3 SV=1)

HSP 1 Score: 104 bits (259), Expect = 8.38e-27
Identity = 49/58 (84.48%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1   LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 58
           L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTI+
Sbjct: 71  LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIV 128

BLAST of MC05g0793 vs. ExPASy TrEMBL
Match: A0A444JHR1 (DNA-directed RNA polymerase (Fragment) OS=Photobacterium chitinilyticum OX=2485123 GN=EDI28_26660 PE=4 SV=1)

HSP 1 Score: 103 bits (257), Expect = 1.07e-26
Identity = 49/58 (84.48%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1   LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 58
           L+   PLRGR+KQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTII
Sbjct: 88  LVTQQPLRGRSKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTII 145

BLAST of MC05g0793 vs. ExPASy TrEMBL
Match: A0A5K1HR94 (DNA-directed RNA polymerase (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS30651 PE=3 SV=1)

HSP 1 Score: 104 bits (259), Expect = 1.54e-26
Identity = 49/58 (84.48%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1   LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 58
           L+   PLRGRAKQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTI+
Sbjct: 131 LVTQQPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTIV 188

BLAST of MC05g0793 vs. TAIR 10
Match: ATCG00190.1 (RNA polymerase subunit beta )

HSP 1 Score: 104.4 bits (259), Expect = 3.2e-23
Identity = 49/58 (84.48%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1    LLHTTPLRGRAKQGGQRVGEIEVWALEGFGVAHILQEMLTYKSDHIRARQDILDTTII 59
            L+   PLRGR+KQGGQRVGE+EVWALEGFGVAHILQEMLTYKSDHIRARQ++L TTII
Sbjct: 970  LVTQQPLRGRSKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLGTTII 1027

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q589C01.5e-2286.21DNA-directed RNA polymerase subunit beta OS=Silene latifolia OX=37657 GN=rpoB PE... [more]
A9LYH72.0e-2286.21DNA-directed RNA polymerase subunit beta OS=Acorus americanus OX=263995 GN=rpoB ... [more]
Q3V5422.0e-2286.21DNA-directed RNA polymerase subunit beta OS=Acorus calamus OX=4465 GN=rpoB PE=3 ... [more]
Q5QA722.0e-2286.21DNA-directed RNA polymerase subunit beta OS=Acorus gramineus OX=55184 GN=rpoB PE... [more]
Q8S8X92.0e-2286.21DNA-directed RNA polymerase subunit beta OS=Atropa belladonna OX=33113 GN=rpoB P... [more]
Match NameE-valueIdentityDescription
BBN70082.12.76e-2786.21hypothetical protein Prudu_1405S000400 [Prunus dulcis][more]
NLZ73073.15.36e-2784.48DNA-directed RNA polymerase subunit beta [Bacteroidales bacterium][more]
VVW90808.11.73e-2684.48unnamed protein product, partial [Nymphaea colorata][more]
WP_199287517.12.21e-2684.48hypothetical protein, partial [Photobacterium chitinilyticum] >RWX52626.1 DNA-di... [more]
VVW90363.13.19e-2684.48unnamed protein product, partial [Nymphaea colorata][more]
Match NameE-valueIdentityDescription
A0A5H2XTM01.34e-2786.21DNA-directed RNA polymerase OS=Prunus dulcis OX=3755 GN=Prudu_1405S000400 PE=3 S... [more]
A0A7X8ZSZ92.59e-2784.48DNA-directed RNA polymerase subunit beta OS=Bacteroidales bacterium OX=2030927 G... [more]
A0A5K1HTK98.38e-2784.48DNA-directed RNA polymerase (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOC... [more]
A0A444JHR11.07e-2684.48DNA-directed RNA polymerase (Fragment) OS=Photobacterium chitinilyticum OX=24851... [more]
A0A5K1HR941.54e-2684.48DNA-directed RNA polymerase (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOC... [more]
Match NameE-valueIdentityDescription
ATCG00190.13.2e-2384.48RNA polymerase subunit beta [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.1800.10RNA polymerase alpha subunit dimerisation domaincoord: 1..52
e-value: 6.3E-13
score: 51.0
NoneNo IPR availablePANTHERPTHR20856:SF30DNA-DIRECTED RNA POLYMERASE SUBUNIT BETAcoord: 6..58
NoneNo IPR availableSUPERFAMILY64484beta and beta-prime subunits of DNA dependent RNA-polymerasecoord: 5..53
IPR007641RNA polymerase Rpb2, domain 7PFAMPF04560RNA_pol_Rpb2_7coord: 14..53
e-value: 3.5E-14
score: 53.0
IPR015712DNA-directed RNA polymerase, subunit 2PANTHERPTHR20856DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2coord: 6..58

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0793.1MC05g0793.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0009536 plastid
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0032549 ribonucleoside binding