MC05g0763 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0763
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionpirin-like protein
LocationMC05: 6247404 .. 6249843 (-)
RNA-Seq ExpressionMC05g0763
SyntenyMC05g0763
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGATTGTGTGTTTGCTTTTTAGTTAATAGTTTTAGTTTTAGAAAGGTGTTCATCATGATGATTCAAGCTTGACATCATATTGATGATTGGTCCAAAATGAATCAAAAAAATAAAACAAAACAAAATAATTTTTATTTAATTATTGGACCCCACTTCTGAATTTTCCCACGATTCCAATCCCCATGCGTCGCCTCTCTGTCAGAACCCCCAACAAACCCAAATCCTCACTCCATTTATATATATATCCCCAAATCCCTAATCAAAATCCCCAATTTCCCAATTCCCAAATTCAGTTCATGATCACAGCCTCTATTTCCAAATTCCCCGCCATTGTTCCTTCCAGATTCTCCTTCTTCCGCTCCGCCGCCGCCTCCTCCGCCATGTCTGAATCCGATCGCTCATCGCCCTTCGCCGCTCCTCGCTTGGTCCTCAAGAAGATTCTCGCCAAGCTCCAGCACGAAGGAGACGGCGCCGTCGTTCGTAGAGGCATTGGAAGGTACTAATTCTTCTCAAATCTTCTTCAATCTTCTTCTCCGCCCACTCATTTCTTCTTCTCTCTGTTCCTCAGAACTGATTTGAAGAATTTGGATCCGTTTCTCATGCTCGACGATTTCTCCGGTACCGGAACCGATTCTCTTGAGATTCATTCGCTTTAATTTCTTCTTCCTCTGTTTTCTGATTCAATTTTCTCTCCGTCGATTCATCTTTTGCAGTCAGTCCGCCGGCTGGATTCCCTGACCATCCTCACAGAGGTTCATTTTCAATTCCTTCCGTTGAATTTTCATACCAACTCCTCTAACAAACTTCTTCTCCTTTTTTTTTTTTTAATGCACGCGTTTTAACTTTGAGCAGAGGTTGTATTTTTTAAAATTCTTGAATATTCTATTTATAAAGTTGATTTTGAAGTCTTGTATTAAATGAAACCGTGTCCTAAAACCACGATTTCCTATATATATGAGTGTGAAATGTCTTTTTTTTTTATTTTTAAAGATTTGGAGTATTTTTAAATTTTTTTGGGGGGCCAAGTATCTTATTTTAAAAATAAGTCTCCTATTTTCTCATATTTTGATGTTGATTTGTTTTTTTTTTAATAGGGTTTGAGACAGTGACCTACATGTTACAGGTAAAATCTGGTTTCTGTGGTGAAATTAAGAAATTATGGTGTGGGGACAAATTTCTTTTTTTTTTCTTTTTTAATTTTGATTATTTATTTGGATTTTAGGGAGGAATTACCCATCAAGATTTTTCTGGCCACAAGGGTACAATCAGGACTGGAGATGTCCAGGTTAAATATGTCATTTCCTTAAAAATATATATATATAGTTTATGTGGCTTTTTTTATTTTTAATTTTTAAATATGATGTATTGAGGAGGTTATTTATTACGTCACAATTGACATTTTGGGTTATTTTGAGGTGGCAGTGGATGACAGCAGGGAGAGGAATTATCCATTCGGAAATGCCTGCTGGAGAAGGAAGTCAAAAGGGCTTGCAGCTTTGGATCAATCTCTCATCTCGTGACAAAACGTAAAAAAAAAAAAAAACTGTTTTAAGAGAGAAATTATTGAATATGATCGATCGTGAATGTTATATTGACAATTACATTGTGGCAATTTGCTAGGATGGAGCCGAGGTATCAGGAGCTGCCAAGCGAGAACATTCCGAGAGCGGAGAAAGACGGGGTTGAAGTTCGCGTCATAGCAGGAGAGTCCATGGGAGTGCACTCTCCAATCTACACACGAACCCCAACCATGTACCTCGACTTCACTCTGAAACCAAGGGCTCAAGTGCACCAAAGCATCCCGGATTCGTGGACCGCATTCGTGTACATAATCGAGGGGGAAGGGGTGTTCGGGGCACCAAGCTCGTCTCGAGCGGGCGCCCACCATGTGCTAGTGCTTGGAGTTGGAGAAGGGGTGAGCGTGTGGAACAGATCTTCAAAGCCATTGAGGTTTGTTCTGATTGCAGGGAAGCCTTTGAATGAACCAATTGTGCAATATGGACCATTTGTGATGAACACACAATCTGAAATTGATCAAGCCATTGAGGATTATCACTACTCCAAAAATGGGTTTGAAATGGCAAAGCATTGGAGATCTCAGTGAGAATAAAAAAAAAGCTAAAAAAAAAAAAGGGAATTTTTTGAAAAGTAAAAAAAAAAAAAAAGAATTGGTCAATGTCTCTATCCAAACAGAGCAAAAATATTTACATTTTAAGGAAAGGAAACCTTCTAGAAGACCCCCAAATGCTCTGCTGAATCTTCTGTTGGGGTCAGCTTATCTTAGTCAAAATTTATGTTTTTATCTATTTTTATTTTATTTTTTTATTTTTACTTTTGTTGGTGGTAAAGGGTTGTAATAGTCTTTTTCTTTTTATGTATTTTTATTATAAATTTTATCAACTATTTTGTTGGCTAAATGTACCTTCTAAGAGTTTAT

mRNA sequence

AAAAGATTGTGTGTTTGCTTTTTAGTTAATAGTTTTAGTTTTAGAAAGGTGTTCATCATGATGATTCAAGCTTGACATCATATTGATGATTGGTCCAAAATGAATCAAAAAAATAAAACAAAACAAAATAATTTTTATTTAATTATTGGACCCCACTTCTGAATTTTCCCACGATTCCAATCCCCATGCGTCGCCTCTCTGTCAGAACCCCCAACAAACCCAAATCCTCACTCCATTTATATATATATCCCCAAATCCCTAATCAAAATCCCCAATTTCCCAATTCCCAAATTCAGTTCATGATCACAGCCTCTATTTCCAAATTCCCCGCCATTGTTCCTTCCAGATTCTCCTTCTTCCGCTCCGCCGCCGCCTCCTCCGCCATGTCTGAATCCGATCGCTCATCGCCCTTCGCCGCTCCTCGCTTGGTCCTCAAGAAGATTCTCGCCAAGCTCCAGCACGAAGGAGACGGCGCCGTCGTTCGTAGAGGCATTGGAAGAACTGATTTGAAGAATTTGGATCCGTTTCTCATGCTCGACGATTTCTCCGTCAGTCCGCCGGCTGGATTCCCTGACCATCCTCACAGAGGGTTTGAGACAGTGACCTACATGTTACAGGGAGGAATTACCCATCAAGATTTTTCTGGCCACAAGGGTACAATCAGGACTGGAGATGTCCAGTGGATGACAGCAGGGAGAGGAATTATCCATTCGGAAATGCCTGCTGGAGAAGGAAGTCAAAAGGGCTTGCAGCTTTGGATCAATCTCTCATCTCGTGACAAAACGATGGAGCCGAGGTATCAGGAGCTGCCAAGCGAGAACATTCCGAGAGCGGAGAAAGACGGGGTTGAAGTTCGCGTCATAGCAGGAGAGTCCATGGGAGTGCACTCTCCAATCTACACACGAACCCCAACCATGTACCTCGACTTCACTCTGAAACCAAGGGCTCAAGTGCACCAAAGCATCCCGGATTCGTGGACCGCATTCGTGTACATAATCGAGGGGGAAGGGGTGTTCGGGGCACCAAGCTCGTCTCGAGCGGGCGCCCACCATGTGCTAGTGCTTGGAGTTGGAGAAGGGGTGAGCGTGTGGAACAGATCTTCAAAGCCATTGAGGTTTGTTCTGATTGCAGGGAAGCCTTTGAATGAACCAATTGTGCAATATGGACCATTTGTGATGAACACACAATCTGAAATTGATCAAGCCATTGAGGATTATCACTACTCCAAAAATGGGTTTGAAATGGCAAAGCATTGGAGATCTCAGTGAGAATAAAAAAAAAGCTAAAAAAAAAAAAGGGAATTTTTTGAAAAGTAAAAAAAAAAAAAAAGAATTGGTCAATGTCTCTATCCAAACAGAGCAAAAATATTTACATTTTAAGGAAAGGAAACCTTCTAGAAGACCCCCAAATGCTCTGCTGAATCTTCTGTTGGGGTCAGCTTATCTTAGTCAAAATTTATGTTTTTATCTATTTTTATTTTATTTTTTTATTTTTACTTTTGTTGGTGGTAAAGGGTTGTAATAGTCTTTTTCTTTTTATGTATTTTTATTATAAATTTTATCAACTATTTTGTTGGCTAAATGTACCTTCTAAGAGTTTAT

Coding sequence (CDS)

ATGCGTCGCCTCTCTGTCAGAACCCCCAACAAACCCAAATCCTCACTCCATTTATATATATATCCCCAAATCCCTAATCAAAATCCCCAATTTCCCAATTCCCAAATTCAGTTCATGATCACAGCCTCTATTTCCAAATTCCCCGCCATTGTTCCTTCCAGATTCTCCTTCTTCCGCTCCGCCGCCGCCTCCTCCGCCATGTCTGAATCCGATCGCTCATCGCCCTTCGCCGCTCCTCGCTTGGTCCTCAAGAAGATTCTCGCCAAGCTCCAGCACGAAGGAGACGGCGCCGTCGTTCGTAGAGGCATTGGAAGAACTGATTTGAAGAATTTGGATCCGTTTCTCATGCTCGACGATTTCTCCGTCAGTCCGCCGGCTGGATTCCCTGACCATCCTCACAGAGGGTTTGAGACAGTGACCTACATGTTACAGGGAGGAATTACCCATCAAGATTTTTCTGGCCACAAGGGTACAATCAGGACTGGAGATGTCCAGTGGATGACAGCAGGGAGAGGAATTATCCATTCGGAAATGCCTGCTGGAGAAGGAAGTCAAAAGGGCTTGCAGCTTTGGATCAATCTCTCATCTCGTGACAAAACGATGGAGCCGAGGTATCAGGAGCTGCCAAGCGAGAACATTCCGAGAGCGGAGAAAGACGGGGTTGAAGTTCGCGTCATAGCAGGAGAGTCCATGGGAGTGCACTCTCCAATCTACACACGAACCCCAACCATGTACCTCGACTTCACTCTGAAACCAAGGGCTCAAGTGCACCAAAGCATCCCGGATTCGTGGACCGCATTCGTGTACATAATCGAGGGGGAAGGGGTGTTCGGGGCACCAAGCTCGTCTCGAGCGGGCGCCCACCATGTGCTAGTGCTTGGAGTTGGAGAAGGGGTGAGCGTGTGGAACAGATCTTCAAAGCCATTGAGGTTTGTTCTGATTGCAGGGAAGCCTTTGAATGAACCAATTGTGCAATATGGACCATTTGTGATGAACACACAATCTGAAATTGATCAAGCCATTGAGGATTATCACTACTCCAAAAATGGGTTTGAAATGGCAAAGCATTGGAGATCTCAGTGA

Protein sequence

MRRLSVRTPNKPKSSLHLYIYPQIPNQNPQFPNSQIQFMITASISKFPAIVPSRFSFFRSAAASSAMSESDRSSPFAAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRTPTMYLDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAPSSSRAGAHHVLVLGVGEGVSVWNRSSKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKNGFEMAKHWRSQ
Homology
BLAST of MC05g0763 vs. ExPASy Swiss-Prot
Match: Q9SEE4 (Pirin-like protein OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 2.0e-128
Identity = 211/288 (73.26%), Postives = 250/288 (86.81%), Query Frame = 0

Query: 73  SSPFAAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFSVSPPAGFPDHP 132
           SS F+ PRLV+KK+LA+ Q+EGDGA+VRR IGR +L+NLDPFLMLD+FSVS PAGFPDHP
Sbjct: 4   SSIFSRPRLVVKKVLARAQNEGDGAIVRRSIGRPELQNLDPFLMLDEFSVSQPAGFPDHP 63

Query: 133 HRGFETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAGEGSQKGLQLWI 192
           HRGFETVTYMLQG  THQDF+GHKGTIRTGDVQWMTAGRGI+HSEMPAG G+QKGLQLWI
Sbjct: 64  HRGFETVTYMLQGAFTHQDFAGHKGTIRTGDVQWMTAGRGIVHSEMPAGPGTQKGLQLWI 123

Query: 193 NLSSRDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRTPTMYLDFTLKP 252
           NLSS+DK +EPRYQEL  ++IP+AEKDGV V ++AGESMG  S ++TRTPTMYLDFTLKP
Sbjct: 124 NLSSKDKMIEPRYQELLHQDIPKAEKDGVSVTILAGESMGKKSQVFTRTPTMYLDFTLKP 183

Query: 253 RAQVHQSIPDSWTAFVYIIEGEGVFGAPSSSRAGAHHVLVLGVGEGVSVWNRSSKPLRFV 312
            ++ HQ IP++W AF+YI+EGEG FG+  S+   AHH LVLG GEG+SVWN+SSKPLRFV
Sbjct: 184 GSEHHQPIPETWNAFLYIVEGEGAFGSSDSTTTPAHHCLVLGPGEGLSVWNKSSKPLRFV 243

Query: 313 LIAGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKNGFEMAKHWRSQ 361
           LI G+P+NEP+VQYGPFVMNT+SEI QA +DY   KNGFE ++ W S+
Sbjct: 244 LIGGQPINEPVVQYGPFVMNTKSEIMQAYQDYQLGKNGFERSRQWYSK 291

BLAST of MC05g0763 vs. ExPASy Swiss-Prot
Match: Q9ZW82 (Pirin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PRN2 PE=1 SV=3)

HSP 1 Score: 436.4 bits (1121), Expect = 3.1e-121
Identity = 216/311 (69.45%), Postives = 253/311 (81.35%), Query Frame = 0

Query: 55  FSFFRSAAASSAMSESDRSSPFAAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPF 114
           F F R+  + S+ +  D  S     R V+KK+ AKLQ EGDGAVVRRGI R++ K LDPF
Sbjct: 17  FRFIRNIKSMSSSTSQDFVS-----RPVIKKVFAKLQKEGDGAVVRRGISRSEQKLLDPF 76

Query: 115 LMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGII 174
           LMLD+FSVSPPAGFPDHPHRGFETVTY+L+GGITHQDF GHKGTI  GDVQWMTAGRGII
Sbjct: 77  LMLDEFSVSPPAGFPDHPHRGFETVTYVLEGGITHQDFKGHKGTIYAGDVQWMTAGRGII 136

Query: 175 HSEMPAGEGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVH 234
           HSEMP  E   KGLQLWINLSS +K +EP YQEL   +IP+AE++GVEV+VIAGESMG+ 
Sbjct: 137 HSEMPE-EEVNKGLQLWINLSSNEKMIEPNYQELSHSDIPKAEQNGVEVKVIAGESMGIQ 196

Query: 235 SPIYTRTPTMYLDFTLKPRAQVHQSIPDSWTAFVYII---EGEGVFGAPSSSRAGAHHVL 294
           SP+YTRTPTM+LDFTL+P AQ+HQ++P+SW AF YI+   EG GVF + +SS   AH V+
Sbjct: 197 SPVYTRTPTMFLDFTLQPGAQIHQNVPESWNAFAYILESGEGGGVFSSSNSSPIPAHSVV 256

Query: 295 VLGVG-EGVSVWNR-SSKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKN 354
           V G G +GVSVWN+ SSK LRFVLIAG+P+ EP+VQYGPFVMNTQ+EID  IEDYHY KN
Sbjct: 257 VFGPGNDGVSVWNKSSSKQLRFVLIAGEPIGEPVVQYGPFVMNTQAEIDMTIEDYHYGKN 316

Query: 355 GFEMAKHWRSQ 361
           GFEMAK+WRSQ
Sbjct: 317 GFEMAKYWRSQ 321

BLAST of MC05g0763 vs. ExPASy Swiss-Prot
Match: Q9LX49 (Pirin-1 OS=Arabidopsis thaliana OX=3702 GN=PRN1 PE=1 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 2.7e-109
Identity = 191/281 (67.97%), Postives = 233/281 (82.92%), Query Frame = 0

Query: 77  AAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGF 136
           + PR+V+KK+LAKL+ EG+GAVVR GI + D K LDPF++L +FS S  AGFPDHPHRGF
Sbjct: 7   SVPRIVIKKVLAKLEKEGEGAVVRNGITKIDQKLLDPFVLLVEFSFSLSAGFPDHPHRGF 66

Query: 137 ETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLSS 196
           E+VTYMLQGGI H+D  GHKGTI+ GDVQWMTAGRGIIHSE P  E    GLQLWINL S
Sbjct: 67  ESVTYMLQGGIIHKDPKGHKGTIQAGDVQWMTAGRGIIHSEFPE-EEVNNGLQLWINLPS 126

Query: 197 RDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRTPTMYLDFTLKPRAQV 256
            +K  EP+Y+EL S +IPRAE++GVEV+VIAG+SMG+ SP+YTRTPTM+LDFTLKP +Q 
Sbjct: 127 TEKMTEPKYKELSSLDIPRAEENGVEVKVIAGDSMGIKSPVYTRTPTMFLDFTLKPGSQT 186

Query: 257 HQSIPDSWTAFVYIIEG-EGVFGAPSSSRAGAHHVLVLGVGEGVSVWNRS-SKPLRFVLI 316
           HQ++P+SWTAF YIIEG EGVFG+ +SS   AHHV+V G G+ VSVWN+S S+ LRF+LI
Sbjct: 187 HQTVPESWTAFAYIIEGDEGVFGSLNSSAISAHHVVVFGPGDLVSVWNKSTSRSLRFLLI 246

Query: 317 AGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKNGFEMAK 356
           AG+P+ EP+VQ GPFVMN+Q+EID A +DY  +KNGFEMAK
Sbjct: 247 AGEPIGEPVVQCGPFVMNSQAEIDMAFDDYQNAKNGFEMAK 286

BLAST of MC05g0763 vs. ExPASy Swiss-Prot
Match: Q9LPS9 (Pirin-like protein At1g50590 OS=Arabidopsis thaliana OX=3702 GN=At1g50590 PE=2 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 6.1e-101
Identity = 183/303 (60.40%), Postives = 225/303 (74.26%), Query Frame = 0

Query: 67  MSESDRSSPFAAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFSVSPPA 126
           M  S++SS     RLV+KK+ A+  HEG GAVVRR IGR + +  DPFL+LD+FSVS PA
Sbjct: 1   MPISEKSSA-TNTRLVVKKLFARQLHEGFGAVVRRSIGRFEFRYFDPFLVLDEFSVSAPA 60

Query: 127 GFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAGEGS-- 186
           GFPDHPHRGFETVTYML+G I H+D  GHKG IR G +QWMTAG+GI+HSEMP+   +  
Sbjct: 61  GFPDHPHRGFETVTYMLEGEILHEDCEGHKGVIREGGLQWMTAGKGIVHSEMPSSNSNGI 120

Query: 187 --QKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRTP 246
              KGLQLWINLSSR K +EP YQE+ S++I   EKDGV VRVIAGE  GV S I TRTP
Sbjct: 121 THNKGLQLWINLSSRQKLVEPSYQEIESKDIAETEKDGVRVRVIAGEWNGVKSKICTRTP 180

Query: 247 TMYLDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAP--SSSRAGAHHVLVLGV-GEGV 306
           TMYLDFTL P +++ Q IP  W AFVY+++G G FG      S A AHH+LVLG+ G+ +
Sbjct: 181 TMYLDFTLSPGSRISQPIPLHWNAFVYVLQGHGHFGDSKLQHSAAAAHHLLVLGLGGDML 240

Query: 307 SVWNRSSK--PLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKNGFEMAKHW 361
             WN S    PLRF+L+AG+P+ EP+VQ+GPFVMNTQ EID+ I+D+   +NGFE A+HW
Sbjct: 241 EAWNGSDSGLPLRFILVAGEPIGEPMVQFGPFVMNTQEEIDETIDDFENFRNGFEKARHW 300

BLAST of MC05g0763 vs. ExPASy Swiss-Prot
Match: Q9LX45 (Putative pirin-like protein At3g59260 OS=Arabidopsis thaliana OX=3702 GN=At3g59260 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.4e-96
Identity = 170/258 (65.89%), Postives = 203/258 (78.68%), Query Frame = 0

Query: 105 RTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDV 164
           ++D + LDPF+ L +FSVSPP GF DHPHRGFE+VTYM QGGI HQD +G+KGTI  GDV
Sbjct: 15  KSDHELLDPFVSLVEFSVSPPGGFKDHPHRGFESVTYMFQGGIIHQDCNGNKGTIHEGDV 74

Query: 165 QWMTAGRGIIHSEMPAGEGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVR 224
           QWMTAGRGIIHSEMP  E   KGLQLWINL S  K +EP+  E+ S  IP A+  GVEV+
Sbjct: 75  QWMTAGRGIIHSEMPE-EQVNKGLQLWINLPSSAKMIEPKNIEISSSEIPSADDYGVEVK 134

Query: 225 VIAGESMGVHSPIYTRTPTMYLDFTLKPRAQVHQSIPDSWTAFVYIIEG-EGVFGAPSSS 284
           VIAGESMGV SP YT+TP M+LDFTL P+AQ HQ++P+SWTAF YI+EG EGVF +  SS
Sbjct: 135 VIAGESMGVKSPFYTKTPIMFLDFTLDPKAQTHQAVPESWTAFAYIVEGDEGVFSSSDSS 194

Query: 285 RAGAHHVLVLGVGEGVSVWNRS-SKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIE 344
              AH+V+V G G+ VSVWN S S+PLRF+LIAG+P+ EP+VQ+GPFVMN+Q EI+  I 
Sbjct: 195 TVQAHNVVVFGTGDEVSVWNTSNSRPLRFLLIAGEPIGEPVVQHGPFVMNSQDEIEMTIG 254

Query: 345 DYHYSKNGFEMAKHWRSQ 361
           DY    NGFEMAKHWRS+
Sbjct: 255 DYRNGMNGFEMAKHWRSE 271

BLAST of MC05g0763 vs. NCBI nr
Match: XP_038879766.1 (pirin-like protein [Benincasa hispida])

HSP 1 Score: 566 bits (1460), Expect = 1.82e-201
Identity = 286/336 (85.12%), Postives = 305/336 (90.77%), Query Frame = 0

Query: 28  NPQFPNSQIQFM--ITASISKFPAIVPSRFSFFRSAAASSAMSESDRSSP-FAAPRLVLK 87
           NP FP   IQFM    + I + PAI+   F FF S+A SSAMS SD S P F  PR V+K
Sbjct: 6   NPFFP-IPIQFMQFSLSPIFRSPAIISPTFFFF-SSAVSSAMSTSDHSPPAFDRPRSVIK 65

Query: 88  KILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQ 147
           KILAKLQHEGDGAVVRRGIGR DLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQ
Sbjct: 66  KILAKLQHEGDGAVVRRGIGRNDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQ 125

Query: 148 GGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLSSRDKTMEPR 207
           GGITHQDF+GHKGTIRTGDVQWMTAGRGIIHSEMPAGEGSQKGLQLWINL+S DK MEPR
Sbjct: 126 GGITHQDFAGHKGTIRTGDVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLASHDKMMEPR 185

Query: 208 YQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRTPTMYLDFTLKPRAQVHQSIPDSW 267
           YQE+ S+NIPRAEKDGVEVRVIAGESMGVHSPIYTRTPTMYLDFTL+PRAQVHQSIPD+W
Sbjct: 186 YQEISSDNIPRAEKDGVEVRVIAGESMGVHSPIYTRTPTMYLDFTLRPRAQVHQSIPDTW 245

Query: 268 TAFVYIIEGEGVFGAPSSSRAGAHHVLVLGVGEGVSVWNRSSKPLRFVLIAGKPLNEPIV 327
           T+FVYIIEGEGVFG+P+SSR  AHHVLVLG G+GVSVWNRSSKPLRFVLIAGKPLNEPIV
Sbjct: 246 TSFVYIIEGEGVFGSPNSSRVAAHHVLVLGGGDGVSVWNRSSKPLRFVLIAGKPLNEPIV 305

Query: 328 QYGPFVMNTQSEIDQAIEDYHYSKNGFEMAKHWRSQ 360
           QYGPFVMN+QSEID+AIEDYHY+KNGFEMAK+WRSQ
Sbjct: 306 QYGPFVMNSQSEIDKAIEDYHYAKNGFEMAKYWRSQ 339

BLAST of MC05g0763 vs. NCBI nr
Match: XP_008453920.1 (PREDICTED: pirin-like protein [Cucumis melo])

HSP 1 Score: 560 bits (1444), Expect = 7.73e-199
Identity = 288/359 (80.22%), Postives = 312/359 (86.91%), Query Frame = 0

Query: 6   VRTPNKPKSSLHLYIYPQIPNQNPQFPNSQIQFM--ITASISKFPAIVPS-RFSFFRSAA 65
           +R PN  +S   + IY     ++P+   SQ  FM    +   K PAI  S +FSF   + 
Sbjct: 1   MRCPNPKESPNPIRIY-----KSPKSSPSQFTFMKFSPSPFFKSPAITVSPKFSF---SI 60

Query: 66  ASSAMSESDRSSP-FAAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFS 125
           AS+AMS SD S P F  PR V+KKILAKLQHEGDGAVVRRGIGR DLKNLDPFLMLDDFS
Sbjct: 61  ASAAMSSSDHSPPAFDRPRSVIKKILAKLQHEGDGAVVRRGIGRNDLKNLDPFLMLDDFS 120

Query: 126 VSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAG 185
           VSPPAGFPDHPHRGFETVTYMLQGGITHQDF+GHKGTIRTGDVQWMTAGRGIIHSEMPAG
Sbjct: 121 VSPPAGFPDHPHRGFETVTYMLQGGITHQDFAGHKGTIRTGDVQWMTAGRGIIHSEMPAG 180

Query: 186 EGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRT 245
           EGSQKGLQLWINL+S DK MEPRYQE+ S+NIPRAEKDGVEVRVIAGESMGVHSPIYTRT
Sbjct: 181 EGSQKGLQLWINLASHDKMMEPRYQEISSDNIPRAEKDGVEVRVIAGESMGVHSPIYTRT 240

Query: 246 PTMYLDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAPSSSRAGAHHVLVLGVGEGVSV 305
           PTMYLDFTL+PRAQVHQSIPD+WT+FVYIIEGEGVFG+ +SSR  AHHVLVLG G+GVSV
Sbjct: 241 PTMYLDFTLRPRAQVHQSIPDTWTSFVYIIEGEGVFGSSNSSRVAAHHVLVLGGGDGVSV 300

Query: 306 WNRSSKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKNGFEMAKHWRSQ 360
           WNRSSKPLRFVLIAGKPLNEPIVQYGPFVMN+QSEIDQAIEDYHY+KNGFEMAK+WRSQ
Sbjct: 301 WNRSSKPLRFVLIAGKPLNEPIVQYGPFVMNSQSEIDQAIEDYHYAKNGFEMAKYWRSQ 351

BLAST of MC05g0763 vs. NCBI nr
Match: XP_004152078.2 (pirin-like protein [Cucumis sativus] >KGN53133.2 hypothetical protein Csa_015206 [Cucumis sativus])

HSP 1 Score: 558 bits (1437), Expect = 3.59e-198
Identity = 274/319 (85.89%), Postives = 296/319 (92.79%), Query Frame = 0

Query: 44  ISKFPAI-VPSRFSFFRSAAASSAMSESDRSSP-FAAPRLVLKKILAKLQHEGDGAVVRR 103
           I K PAI +P +FSF  S + ++AMS SD S P F  PR V+KKILAKLQHEGDGAVVRR
Sbjct: 8   IFKSPAITIPPKFSFSFSFSVAAAMSSSDHSPPAFDRPRSVIKKILAKLQHEGDGAVVRR 67

Query: 104 GIGRTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRT 163
           GIGR DLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDF+GHKGTIRT
Sbjct: 68  GIGRNDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFAGHKGTIRT 127

Query: 164 GDVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGV 223
           GDVQWMTAGRGIIHSEMPAGEGSQKGLQLWINL+S DK MEPRYQE+ S+NIPRAEKDGV
Sbjct: 128 GDVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLASHDKMMEPRYQEISSDNIPRAEKDGV 187

Query: 224 EVRVIAGESMGVHSPIYTRTPTMYLDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAPS 283
           EVRVIAGESMGVHSPIYTRTPTMYLDF+L+PRAQVHQSIPD+WT+FVYIIEGEGVFG+ +
Sbjct: 188 EVRVIAGESMGVHSPIYTRTPTMYLDFSLRPRAQVHQSIPDTWTSFVYIIEGEGVFGSSN 247

Query: 284 SSRAGAHHVLVLGVGEGVSVWNRSSKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAI 343
           SSR  AHHVLVLG G+G+SVWNRSSKPLRFVL+AGKPLNEPIVQYGPFVMN+QSEIDQAI
Sbjct: 248 SSRVAAHHVLVLGGGDGLSVWNRSSKPLRFVLVAGKPLNEPIVQYGPFVMNSQSEIDQAI 307

Query: 344 EDYHYSKNGFEMAKHWRSQ 360
           EDYHY+KNGFEMAK+WRSQ
Sbjct: 308 EDYHYAKNGFEMAKYWRSQ 326

BLAST of MC05g0763 vs. NCBI nr
Match: XP_022932582.1 (pirin-like protein [Cucurbita moschata] >KAG6597627.1 hypothetical protein SDJN03_10807, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 546 bits (1408), Expect = 9.03e-194
Identity = 264/318 (83.02%), Postives = 291/318 (91.51%), Query Frame = 0

Query: 44  ISKFPAIVPSRFSFFRSAAASSAMSESDRSSP-FAAPRLVLKKILAKLQHEGDGAVVRRG 103
           I K PAI+  RFS   + A S+ MS SD SSP F  PR V+KK+LAKLQHEGDGAVVRRG
Sbjct: 8   IFKLPAILSPRFSIIPTTAISATMSTSDHSSPAFDRPRSVIKKVLAKLQHEGDGAVVRRG 67

Query: 104 IGRTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTG 163
           IGR+DLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDF+GHKGTIRTG
Sbjct: 68  IGRSDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFAGHKGTIRTG 127

Query: 164 DVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVE 223
           DVQWMTAGRGIIHSEMPAGEG QKGLQLWINL++ DK MEP+YQE+ S++IPRAE DGVE
Sbjct: 128 DVQWMTAGRGIIHSEMPAGEGPQKGLQLWINLAAHDKMMEPKYQEILSDDIPRAENDGVE 187

Query: 224 VRVIAGESMGVHSPIYTRTPTMYLDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAPSS 283
           V VIAGESMGVHSPIYT+TPTMYLDFTLKP+AQVHQSIPD+WT+FVYII+GEGVFG+ +S
Sbjct: 188 VNVIAGESMGVHSPIYTQTPTMYLDFTLKPKAQVHQSIPDTWTSFVYIIDGEGVFGSLNS 247

Query: 284 SRAGAHHVLVLGVGEGVSVWNRSSKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIE 343
           SR  AHHVLVLG G+G+SVWNRSS PLRF+L+AGKPLNEPIVQ+GPFVMN+QSEIDQAIE
Sbjct: 248 SRVAAHHVLVLGPGDGLSVWNRSSNPLRFILVAGKPLNEPIVQHGPFVMNSQSEIDQAIE 307

Query: 344 DYHYSKNGFEMAKHWRSQ 360
           DYHY+KNGFEMAKHWRSQ
Sbjct: 308 DYHYAKNGFEMAKHWRSQ 325

BLAST of MC05g0763 vs. NCBI nr
Match: XP_023540122.1 (pirin-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 546 bits (1408), Expect = 9.03e-194
Identity = 264/318 (83.02%), Postives = 291/318 (91.51%), Query Frame = 0

Query: 44  ISKFPAIVPSRFSFFRSAAASSAMSESDRSSP-FAAPRLVLKKILAKLQHEGDGAVVRRG 103
           I K PAI+  RFS   + A S+ MS SD SSP F  PR V+KK+LAKLQHEGDGAVVRRG
Sbjct: 8   IFKLPAILSPRFSIIPTTAISATMSTSDHSSPAFDRPRSVIKKVLAKLQHEGDGAVVRRG 67

Query: 104 IGRTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTG 163
           IGR+DLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDF+GHKGTIRTG
Sbjct: 68  IGRSDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFAGHKGTIRTG 127

Query: 164 DVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVE 223
           DVQWMTAGRGIIHSEMPAGEG QKGLQLWINL++ DK MEP+YQE+ SE+IPRAE DGVE
Sbjct: 128 DVQWMTAGRGIIHSEMPAGEGPQKGLQLWINLAAHDKMMEPKYQEILSEDIPRAENDGVE 187

Query: 224 VRVIAGESMGVHSPIYTRTPTMYLDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAPSS 283
           V VIAGESMGVHSPIYT+TPTMYLDFTLKP+AQVHQSIPD+WT+FVYII+GEGVFG+ +S
Sbjct: 188 VNVIAGESMGVHSPIYTQTPTMYLDFTLKPKAQVHQSIPDTWTSFVYIIDGEGVFGSLNS 247

Query: 284 SRAGAHHVLVLGVGEGVSVWNRSSKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIE 343
           SR  AHHVLVLG G+G+SVWN+SS PLRF+L+AGKPLNEPIVQ+GPFVMN+QSEIDQAIE
Sbjct: 248 SRVAAHHVLVLGPGDGLSVWNKSSNPLRFILVAGKPLNEPIVQHGPFVMNSQSEIDQAIE 307

Query: 344 DYHYSKNGFEMAKHWRSQ 360
           DYHY+KNGFEMAKHWRSQ
Sbjct: 308 DYHYAKNGFEMAKHWRSQ 325

BLAST of MC05g0763 vs. ExPASy TrEMBL
Match: A0A1S3BXE1 (pirin-like protein OS=Cucumis melo OX=3656 GN=LOC103494500 PE=3 SV=1)

HSP 1 Score: 560 bits (1444), Expect = 3.74e-199
Identity = 288/359 (80.22%), Postives = 312/359 (86.91%), Query Frame = 0

Query: 6   VRTPNKPKSSLHLYIYPQIPNQNPQFPNSQIQFM--ITASISKFPAIVPS-RFSFFRSAA 65
           +R PN  +S   + IY     ++P+   SQ  FM    +   K PAI  S +FSF   + 
Sbjct: 1   MRCPNPKESPNPIRIY-----KSPKSSPSQFTFMKFSPSPFFKSPAITVSPKFSF---SI 60

Query: 66  ASSAMSESDRSSP-FAAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFS 125
           AS+AMS SD S P F  PR V+KKILAKLQHEGDGAVVRRGIGR DLKNLDPFLMLDDFS
Sbjct: 61  ASAAMSSSDHSPPAFDRPRSVIKKILAKLQHEGDGAVVRRGIGRNDLKNLDPFLMLDDFS 120

Query: 126 VSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAG 185
           VSPPAGFPDHPHRGFETVTYMLQGGITHQDF+GHKGTIRTGDVQWMTAGRGIIHSEMPAG
Sbjct: 121 VSPPAGFPDHPHRGFETVTYMLQGGITHQDFAGHKGTIRTGDVQWMTAGRGIIHSEMPAG 180

Query: 186 EGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRT 245
           EGSQKGLQLWINL+S DK MEPRYQE+ S+NIPRAEKDGVEVRVIAGESMGVHSPIYTRT
Sbjct: 181 EGSQKGLQLWINLASHDKMMEPRYQEISSDNIPRAEKDGVEVRVIAGESMGVHSPIYTRT 240

Query: 246 PTMYLDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAPSSSRAGAHHVLVLGVGEGVSV 305
           PTMYLDFTL+PRAQVHQSIPD+WT+FVYIIEGEGVFG+ +SSR  AHHVLVLG G+GVSV
Sbjct: 241 PTMYLDFTLRPRAQVHQSIPDTWTSFVYIIEGEGVFGSSNSSRVAAHHVLVLGGGDGVSV 300

Query: 306 WNRSSKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKNGFEMAKHWRSQ 360
           WNRSSKPLRFVLIAGKPLNEPIVQYGPFVMN+QSEIDQAIEDYHY+KNGFEMAK+WRSQ
Sbjct: 301 WNRSSKPLRFVLIAGKPLNEPIVQYGPFVMNSQSEIDQAIEDYHYAKNGFEMAKYWRSQ 351

BLAST of MC05g0763 vs. ExPASy TrEMBL
Match: A0A6J1EX63 (pirin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111439095 PE=3 SV=1)

HSP 1 Score: 546 bits (1408), Expect = 4.37e-194
Identity = 264/318 (83.02%), Postives = 291/318 (91.51%), Query Frame = 0

Query: 44  ISKFPAIVPSRFSFFRSAAASSAMSESDRSSP-FAAPRLVLKKILAKLQHEGDGAVVRRG 103
           I K PAI+  RFS   + A S+ MS SD SSP F  PR V+KK+LAKLQHEGDGAVVRRG
Sbjct: 8   IFKLPAILSPRFSIIPTTAISATMSTSDHSSPAFDRPRSVIKKVLAKLQHEGDGAVVRRG 67

Query: 104 IGRTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTG 163
           IGR+DLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDF+GHKGTIRTG
Sbjct: 68  IGRSDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFAGHKGTIRTG 127

Query: 164 DVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVE 223
           DVQWMTAGRGIIHSEMPAGEG QKGLQLWINL++ DK MEP+YQE+ S++IPRAE DGVE
Sbjct: 128 DVQWMTAGRGIIHSEMPAGEGPQKGLQLWINLAAHDKMMEPKYQEILSDDIPRAENDGVE 187

Query: 224 VRVIAGESMGVHSPIYTRTPTMYLDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAPSS 283
           V VIAGESMGVHSPIYT+TPTMYLDFTLKP+AQVHQSIPD+WT+FVYII+GEGVFG+ +S
Sbjct: 188 VNVIAGESMGVHSPIYTQTPTMYLDFTLKPKAQVHQSIPDTWTSFVYIIDGEGVFGSLNS 247

Query: 284 SRAGAHHVLVLGVGEGVSVWNRSSKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIE 343
           SR  AHHVLVLG G+G+SVWNRSS PLRF+L+AGKPLNEPIVQ+GPFVMN+QSEIDQAIE
Sbjct: 248 SRVAAHHVLVLGPGDGLSVWNRSSNPLRFILVAGKPLNEPIVQHGPFVMNSQSEIDQAIE 307

Query: 344 DYHYSKNGFEMAKHWRSQ 360
           DYHY+KNGFEMAKHWRSQ
Sbjct: 308 DYHYAKNGFEMAKHWRSQ 325

BLAST of MC05g0763 vs. ExPASy TrEMBL
Match: A0A6J1I7X3 (pirin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111470813 PE=3 SV=1)

HSP 1 Score: 543 bits (1400), Expect = 7.22e-193
Identity = 264/320 (82.50%), Postives = 291/320 (90.94%), Query Frame = 0

Query: 42  ASISKFPAIVPSRFSFFRSAAASSAMSESDRSSP-FAAPRLVLKKILAKLQHEGDGAVVR 101
           A I K PAI+  RFS   + A S+ MS SD SSP F  PR V+KK+LAKLQHEGDGAVVR
Sbjct: 6   ALIFKLPAILFPRFSIIPTTAISATMSTSDHSSPAFDRPRSVVKKVLAKLQHEGDGAVVR 65

Query: 102 RGIGRTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIR 161
           RGIGR+DLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDF+GHKGTIR
Sbjct: 66  RGIGRSDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFAGHKGTIR 125

Query: 162 TGDVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDG 221
           TGDVQWMTAGRGIIHSEMPAGEG QKGLQLWINL++ DK MEP+YQE+ S +IPRAE DG
Sbjct: 126 TGDVQWMTAGRGIIHSEMPAGEGPQKGLQLWINLAAHDKMMEPKYQEILSNDIPRAENDG 185

Query: 222 VEVRVIAGESMGVHSPIYTRTPTMYLDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAP 281
           VEV VIAGESMGVHSPIYT+TPTMYLDFTLKP+AQVHQSIP++WT+FVYII+GEGVFG+ 
Sbjct: 186 VEVNVIAGESMGVHSPIYTQTPTMYLDFTLKPKAQVHQSIPETWTSFVYIIDGEGVFGSL 245

Query: 282 SSSRAGAHHVLVLGVGEGVSVWNRSSKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQA 341
           +SSR  AHHVLVLG G+G+SVWNRSS PLRF+L+AGKPLNEPIVQ+GPFVMN+QSEIDQA
Sbjct: 246 NSSRVAAHHVLVLGPGDGLSVWNRSSNPLRFILVAGKPLNEPIVQHGPFVMNSQSEIDQA 305

Query: 342 IEDYHYSKNGFEMAKHWRSQ 360
           IEDYHY+KNGFEMAKHWRSQ
Sbjct: 306 IEDYHYAKNGFEMAKHWRSQ 325

BLAST of MC05g0763 vs. ExPASy TrEMBL
Match: A0A6J1GWD7 (pirin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111457750 PE=3 SV=1)

HSP 1 Score: 531 bits (1367), Expect = 2.55e-188
Identity = 257/295 (87.12%), Postives = 276/295 (93.56%), Query Frame = 0

Query: 67  MSESDRS-SPFAAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFSVSPP 126
           MS S  S S F  PR V+KKILAKLQ+EGDGAVVRRGIGR DLKNLDPFLMLDDFSV+PP
Sbjct: 1   MSSSSHSPSAFDRPRSVIKKILAKLQNEGDGAVVRRGIGRNDLKNLDPFLMLDDFSVTPP 60

Query: 127 AGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAGEGSQ 186
           AGFPDHPHRGFETVTYMLQGGITHQDF+GHKGTIRTGDVQWMTAGRGI+HSEMPAG GSQ
Sbjct: 61  AGFPDHPHRGFETVTYMLQGGITHQDFAGHKGTIRTGDVQWMTAGRGIVHSEMPAGAGSQ 120

Query: 187 KGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRTPTMY 246
           KGLQLWINL+SRDK M+PRYQE+PS+NIPRAEKDGVEVRVIAGESMGV SPIYT+TPTMY
Sbjct: 121 KGLQLWINLASRDKMMKPRYQEIPSDNIPRAEKDGVEVRVIAGESMGVRSPIYTQTPTMY 180

Query: 247 LDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAPSSSRAGAHHVLVLGVGEGVSVWNRS 306
           LDFTLKPRAQVHQSIPD+WT+FVYII+GEGVFG+P+SS   AHHVLVLG G GVSVWN+S
Sbjct: 181 LDFTLKPRAQVHQSIPDTWTSFVYIIDGEGVFGSPNSSPVAAHHVLVLGGGNGVSVWNKS 240

Query: 307 SKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKNGFEMAKHWRSQ 360
           SKPLRFVLIAGKPLNEPIVQYGPFVMN+QSEI QAIEDY Y+KNGFEMAK+WRSQ
Sbjct: 241 SKPLRFVLIAGKPLNEPIVQYGPFVMNSQSEIAQAIEDYRYAKNGFEMAKYWRSQ 295

BLAST of MC05g0763 vs. ExPASy TrEMBL
Match: A0A6J1IS18 (pirin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111479446 PE=3 SV=1)

HSP 1 Score: 529 bits (1363), Expect = 1.03e-187
Identity = 257/295 (87.12%), Postives = 274/295 (92.88%), Query Frame = 0

Query: 67  MSESDRS-SPFAAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFSVSPP 126
           MS S  S S F  PR V+KKILAKLQ+EGDGAVVRRGIGR DLKNLDPFLMLDDFSVSPP
Sbjct: 1   MSSSSHSPSAFDRPRSVIKKILAKLQNEGDGAVVRRGIGRNDLKNLDPFLMLDDFSVSPP 60

Query: 127 AGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAGEGSQ 186
           AGFPDHPHRGFETVTYMLQGGITHQDF+GHKGTIRTGDVQWMTAGRGI+HSEMPAG GSQ
Sbjct: 61  AGFPDHPHRGFETVTYMLQGGITHQDFAGHKGTIRTGDVQWMTAGRGIVHSEMPAGAGSQ 120

Query: 187 KGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRTPTMY 246
           KGLQLWINL+SRDK M+PRYQE+PS+NIPRAEKDGVEVRVIAGESMGV SPIYT+TPTMY
Sbjct: 121 KGLQLWINLASRDKMMKPRYQEIPSDNIPRAEKDGVEVRVIAGESMGVRSPIYTQTPTMY 180

Query: 247 LDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAPSSSRAGAHHVLVLGVGEGVSVWNRS 306
           LDF LKPRAQVHQSIPD+WT+FVYII+GEGVFG+PSSS   AHHVLVLG G GVSVWN+S
Sbjct: 181 LDFILKPRAQVHQSIPDTWTSFVYIIDGEGVFGSPSSSPVAAHHVLVLGGGNGVSVWNKS 240

Query: 307 SKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKNGFEMAKHWRSQ 360
           SKPLRFVLIAGKPLNEPIVQYGPFVMN+QSEI QA EDY Y+KNGFEMAK+WRSQ
Sbjct: 241 SKPLRFVLIAGKPLNEPIVQYGPFVMNSQSEIAQAFEDYRYAKNGFEMAKYWRSQ 295

BLAST of MC05g0763 vs. TAIR 10
Match: AT2G43120.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 436.4 bits (1121), Expect = 2.2e-122
Identity = 216/311 (69.45%), Postives = 253/311 (81.35%), Query Frame = 0

Query: 55  FSFFRSAAASSAMSESDRSSPFAAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPF 114
           F F R+  + S+ +  D  S     R V+KK+ AKLQ EGDGAVVRRGI R++ K LDPF
Sbjct: 17  FRFIRNIKSMSSSTSQDFVS-----RPVIKKVFAKLQKEGDGAVVRRGISRSEQKLLDPF 76

Query: 115 LMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGII 174
           LMLD+FSVSPPAGFPDHPHRGFETVTY+L+GGITHQDF GHKGTI  GDVQWMTAGRGII
Sbjct: 77  LMLDEFSVSPPAGFPDHPHRGFETVTYVLEGGITHQDFKGHKGTIYAGDVQWMTAGRGII 136

Query: 175 HSEMPAGEGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVH 234
           HSEMP  E   KGLQLWINLSS +K +EP YQEL   +IP+AE++GVEV+VIAGESMG+ 
Sbjct: 137 HSEMPE-EEVNKGLQLWINLSSNEKMIEPNYQELSHSDIPKAEQNGVEVKVIAGESMGIQ 196

Query: 235 SPIYTRTPTMYLDFTLKPRAQVHQSIPDSWTAFVYII---EGEGVFGAPSSSRAGAHHVL 294
           SP+YTRTPTM+LDFTL+P AQ+HQ++P+SW AF YI+   EG GVF + +SS   AH V+
Sbjct: 197 SPVYTRTPTMFLDFTLQPGAQIHQNVPESWNAFAYILESGEGGGVFSSSNSSPIPAHSVV 256

Query: 295 VLGVG-EGVSVWNR-SSKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKN 354
           V G G +GVSVWN+ SSK LRFVLIAG+P+ EP+VQYGPFVMNTQ+EID  IEDYHY KN
Sbjct: 257 VFGPGNDGVSVWNKSSSKQLRFVLIAGEPIGEPVVQYGPFVMNTQAEIDMTIEDYHYGKN 316

Query: 355 GFEMAKHWRSQ 361
           GFEMAK+WRSQ
Sbjct: 317 GFEMAKYWRSQ 321

BLAST of MC05g0763 vs. TAIR 10
Match: AT3G59220.1 (pirin )

HSP 1 Score: 396.7 bits (1018), Expect = 1.9e-110
Identity = 191/281 (67.97%), Postives = 233/281 (82.92%), Query Frame = 0

Query: 77  AAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGF 136
           + PR+V+KK+LAKL+ EG+GAVVR GI + D K LDPF++L +FS S  AGFPDHPHRGF
Sbjct: 7   SVPRIVIKKVLAKLEKEGEGAVVRNGITKIDQKLLDPFVLLVEFSFSLSAGFPDHPHRGF 66

Query: 137 ETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAGEGSQKGLQLWINLSS 196
           E+VTYMLQGGI H+D  GHKGTI+ GDVQWMTAGRGIIHSE P  E    GLQLWINL S
Sbjct: 67  ESVTYMLQGGIIHKDPKGHKGTIQAGDVQWMTAGRGIIHSEFPE-EEVNNGLQLWINLPS 126

Query: 197 RDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRTPTMYLDFTLKPRAQV 256
            +K  EP+Y+EL S +IPRAE++GVEV+VIAG+SMG+ SP+YTRTPTM+LDFTLKP +Q 
Sbjct: 127 TEKMTEPKYKELSSLDIPRAEENGVEVKVIAGDSMGIKSPVYTRTPTMFLDFTLKPGSQT 186

Query: 257 HQSIPDSWTAFVYIIEG-EGVFGAPSSSRAGAHHVLVLGVGEGVSVWNRS-SKPLRFVLI 316
           HQ++P+SWTAF YIIEG EGVFG+ +SS   AHHV+V G G+ VSVWN+S S+ LRF+LI
Sbjct: 187 HQTVPESWTAFAYIIEGDEGVFGSLNSSAISAHHVVVFGPGDLVSVWNKSTSRSLRFLLI 246

Query: 317 AGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKNGFEMAK 356
           AG+P+ EP+VQ GPFVMN+Q+EID A +DY  +KNGFEMAK
Sbjct: 247 AGEPIGEPVVQCGPFVMNSQAEIDMAFDDYQNAKNGFEMAK 286

BLAST of MC05g0763 vs. TAIR 10
Match: AT1G50590.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 369.0 bits (946), Expect = 4.3e-102
Identity = 183/303 (60.40%), Postives = 225/303 (74.26%), Query Frame = 0

Query: 67  MSESDRSSPFAAPRLVLKKILAKLQHEGDGAVVRRGIGRTDLKNLDPFLMLDDFSVSPPA 126
           M  S++SS     RLV+KK+ A+  HEG GAVVRR IGR + +  DPFL+LD+FSVS PA
Sbjct: 1   MPISEKSSA-TNTRLVVKKLFARQLHEGFGAVVRRSIGRFEFRYFDPFLVLDEFSVSAPA 60

Query: 127 GFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDVQWMTAGRGIIHSEMPAGEGS-- 186
           GFPDHPHRGFETVTYML+G I H+D  GHKG IR G +QWMTAG+GI+HSEMP+   +  
Sbjct: 61  GFPDHPHRGFETVTYMLEGEILHEDCEGHKGVIREGGLQWMTAGKGIVHSEMPSSNSNGI 120

Query: 187 --QKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVRVIAGESMGVHSPIYTRTP 246
              KGLQLWINLSSR K +EP YQE+ S++I   EKDGV VRVIAGE  GV S I TRTP
Sbjct: 121 THNKGLQLWINLSSRQKLVEPSYQEIESKDIAETEKDGVRVRVIAGEWNGVKSKICTRTP 180

Query: 247 TMYLDFTLKPRAQVHQSIPDSWTAFVYIIEGEGVFGAP--SSSRAGAHHVLVLGV-GEGV 306
           TMYLDFTL P +++ Q IP  W AFVY+++G G FG      S A AHH+LVLG+ G+ +
Sbjct: 181 TMYLDFTLSPGSRISQPIPLHWNAFVYVLQGHGHFGDSKLQHSAAAAHHLLVLGLGGDML 240

Query: 307 SVWNRSSK--PLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIEDYHYSKNGFEMAKHW 361
             WN S    PLRF+L+AG+P+ EP+VQ+GPFVMNTQ EID+ I+D+   +NGFE A+HW
Sbjct: 241 EAWNGSDSGLPLRFILVAGEPIGEPMVQFGPFVMNTQEEIDETIDDFENFRNGFEKARHW 300

BLAST of MC05g0763 vs. TAIR 10
Match: AT3G59260.1 (pirin, putative )

HSP 1 Score: 353.2 bits (905), Expect = 2.5e-97
Identity = 170/258 (65.89%), Postives = 203/258 (78.68%), Query Frame = 0

Query: 105 RTDLKNLDPFLMLDDFSVSPPAGFPDHPHRGFETVTYMLQGGITHQDFSGHKGTIRTGDV 164
           ++D + LDPF+ L +FSVSPP GF DHPHRGFE+VTYM QGGI HQD +G+KGTI  GDV
Sbjct: 15  KSDHELLDPFVSLVEFSVSPPGGFKDHPHRGFESVTYMFQGGIIHQDCNGNKGTIHEGDV 74

Query: 165 QWMTAGRGIIHSEMPAGEGSQKGLQLWINLSSRDKTMEPRYQELPSENIPRAEKDGVEVR 224
           QWMTAGRGIIHSEMP  E   KGLQLWINL S  K +EP+  E+ S  IP A+  GVEV+
Sbjct: 75  QWMTAGRGIIHSEMPE-EQVNKGLQLWINLPSSAKMIEPKNIEISSSEIPSADDYGVEVK 134

Query: 225 VIAGESMGVHSPIYTRTPTMYLDFTLKPRAQVHQSIPDSWTAFVYIIEG-EGVFGAPSSS 284
           VIAGESMGV SP YT+TP M+LDFTL P+AQ HQ++P+SWTAF YI+EG EGVF +  SS
Sbjct: 135 VIAGESMGVKSPFYTKTPIMFLDFTLDPKAQTHQAVPESWTAFAYIVEGDEGVFSSSDSS 194

Query: 285 RAGAHHVLVLGVGEGVSVWNRS-SKPLRFVLIAGKPLNEPIVQYGPFVMNTQSEIDQAIE 344
              AH+V+V G G+ VSVWN S S+PLRF+LIAG+P+ EP+VQ+GPFVMN+Q EI+  I 
Sbjct: 195 TVQAHNVVVFGTGDEVSVWNTSNSRPLRFLLIAGEPIGEPVVQHGPFVMNSQDEIEMTIG 254

Query: 345 DYHYSKNGFEMAKHWRSQ 361
           DY    NGFEMAKHWRS+
Sbjct: 255 DYRNGMNGFEMAKHWRSE 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SEE42.0e-12873.26Pirin-like protein OS=Solanum lycopersicum OX=4081 PE=2 SV=1[more]
Q9ZW823.1e-12169.45Pirin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PRN2 PE=1 SV=3[more]
Q9LX492.7e-10967.97Pirin-1 OS=Arabidopsis thaliana OX=3702 GN=PRN1 PE=1 SV=1[more]
Q9LPS96.1e-10160.40Pirin-like protein At1g50590 OS=Arabidopsis thaliana OX=3702 GN=At1g50590 PE=2 S... [more]
Q9LX453.4e-9665.89Putative pirin-like protein At3g59260 OS=Arabidopsis thaliana OX=3702 GN=At3g592... [more]
Match NameE-valueIdentityDescription
XP_038879766.11.82e-20185.12pirin-like protein [Benincasa hispida][more]
XP_008453920.17.73e-19980.22PREDICTED: pirin-like protein [Cucumis melo][more]
XP_004152078.23.59e-19885.89pirin-like protein [Cucumis sativus] >KGN53133.2 hypothetical protein Csa_015206... [more]
XP_022932582.19.03e-19483.02pirin-like protein [Cucurbita moschata] >KAG6597627.1 hypothetical protein SDJN0... [more]
XP_023540122.19.03e-19483.02pirin-like protein [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A1S3BXE13.74e-19980.22pirin-like protein OS=Cucumis melo OX=3656 GN=LOC103494500 PE=3 SV=1[more]
A0A6J1EX634.37e-19483.02pirin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111439095 PE=3 SV=1[more]
A0A6J1I7X37.22e-19382.50pirin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111470813 PE=3 SV=1[more]
A0A6J1GWD72.55e-18887.12pirin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111457750 PE=3 SV=1[more]
A0A6J1IS181.03e-18787.12pirin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111479446 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G43120.12.2e-12269.45RmlC-like cupins superfamily protein [more]
AT3G59220.11.9e-11067.97pirin [more]
AT1G50590.14.3e-10260.40RmlC-like cupins superfamily protein [more]
AT3G59260.12.5e-9765.89pirin, putative [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 214..319
e-value: 5.2E-110
score: 369.1
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 93..345
e-value: 5.2E-110
score: 369.1
IPR003829Pirin, N-terminal domainPFAMPF02678Pirincoord: 100..192
e-value: 1.7E-32
score: 111.6
IPR008778Pirin, C-terminal domainPFAMPF05726Pirin_Ccoord: 245..349
e-value: 1.1E-34
score: 119.0
IPR012093PirinPANTHERPTHR13903PIRIN-RELATEDcoord: 72..360
NoneNo IPR availablePANTHERPTHR13903:SF21PIRIN-LIKE PROTEINcoord: 72..360
NoneNo IPR availableCDDcd02247cupin_pirin_Ccoord: 242..318
e-value: 2.62177E-24
score: 92.6794
NoneNo IPR availableCDDcd02909cupin_pirin_Ncoord: 97..194
e-value: 1.99555E-58
score: 182.735
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 80..358

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0763.1MC05g0763.1mRNA