MC05g0757 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0757 vs. ExPASy Swiss-Prot
Match: Q9MA46 (Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGL PE=1 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.6e-138
Identity = 266/455 (58.46%), Postives = 329/455 (72.31%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSSRQLVSSSSVLV 60
           MAA     NPI       + +    H+    S+     S  +V S S+    +SSS + +
Sbjct: 1   MAAKVFTQNPIYSQSLVRDKTPQQKHNLDHFSIS-QHTSKRLVVSSSTMSPPISSSPLSL 60

Query: 61  P---LIDVFSPATAAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHPLLRREVIRYGEFV 120
           P         P+ A AV      L+R+WR+I G +NWE+L+EP L P+L++E+ RYG  +
Sbjct: 61  PSSSSSQAIPPSRAPAV---TLPLSRVWREIQGSNNWENLIEP-LSPILQQEITRYGNLL 120

Query: 121 TVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGL-ESSGYEVTKYIYATPPDINIPPIQNSP 180
           +  YK FDL+PNSKRYL+CK+GK++LLKE G+ +  GY+VTKYIYAT PDIN+ PI+N P
Sbjct: 121 SASYKGFDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYIYAT-PDINLNPIKNEP 180

Query: 181 PCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPHNQRPD 240
               RWIGYVAVSSDE+ K LGRRDI++TFRGTVTN EW+ANL SSLTPARLDPHN RPD
Sbjct: 181 N-RARWIGYVAVSSDESVKRLGRRDILVTFRGTVTNHEWLANLKSSLTPARLDPHNPRPD 240

Query: 241 VKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALAV 300
           VKVESGFL LYTS E+  KFGLESCREQLLSE+SRL+NK+K EE+SIT+AGHSMGS+LA 
Sbjct: 241 VKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKHKGEEISITLAGHSMGSSLAQ 300

Query: 301 LLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPIT 360
           LLAYDIAELG+N+R + K  VPV+VFSF GPRVGN GFK+RCEELGVKVLRI NVNDPIT
Sbjct: 301 LLAYDIAELGMNQRRDEKP-VPVTVFSFAGPRVGNLGFKKRCEELGVKVLRITNVNDPIT 360

Query: 361 KMPGVLFNENFRVFGGLLNGGGNNS-YEHVGVELVLDFFNMQNPSCVHDLETYISLLRSP 420
           K+PG LFNENFR  GG+     + S Y HVGVEL LDFF++QN SCVHDLETYI+L+  P
Sbjct: 361 KLPGFLFNENFRSLGGVYELPWSCSCYTHVGVELTLDFFDVQNISCVHDLETYITLVNRP 420

Query: 421 KEEEQEEENGRIIRKNNSGGDFINRAMEFFCSNAQ 451
           +  +        + ++N GG+F+NR  E   S  +
Sbjct: 421 RCSK------LAVNEDNFGGEFLNRTSELMFSKGR 441

BLAST of MC05g0757 vs. ExPASy Swiss-Prot
Match: Q9SIN9 (Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL5 PE=1 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 3.1e-137
Identity = 270/471 (57.32%), Postives = 329/471 (69.85%), Query Frame = 0

Query: 9   NPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSSRQLVSSSSVLVPLI----- 68
           NP   H  F     P    F   +L LP    N     +++    SSSS+L P+I     
Sbjct: 6   NPNMKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSS--SSSSLLAPVILNSPV 65

Query: 69  -----------DVFSPATAAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHPLLRREVIR 128
                        F  ++ AA V     L+R+WR+I GC+NW+DL+EP L+PLL++E+ R
Sbjct: 66  ASSSPPPIYCAPKFPCSSGAATV----PLSRVWREIQGCNNWKDLIEP-LNPLLQQEITR 125

Query: 129 YGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLES-SGYEVTKYIYATPP-DINIP 188
           YG  V+ CYKAFDLDPNSKRYL CK+GK++LLKE  ++    Y+VTKYIYATP  +INI 
Sbjct: 126 YGNLVSTCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININIS 185

Query: 189 PIQNSPPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDP 248
           PIQN      RW+GYVA SSD++ K LGRRDIV+TFRGTVTN EW+AN MSSLTPAR  P
Sbjct: 186 PIQNEMNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHP 245

Query: 249 HNQRPDVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSM 308
           HN R DVKVESGFL+LYTS+E+  KFGLESCR+QLLSE+SRL+NKYK EE+SIT+AGHSM
Sbjct: 246 HNPRLDVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSM 305

Query: 309 GSALAVLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVN 368
           GS+LA LLAYDIAELGLNRR+  K  +PV+VFSF GPRVGN  FK+RCEELGVKVLRI N
Sbjct: 306 GSSLAQLLAYDIAELGLNRRI-GKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITN 365

Query: 369 VNDPITKMPGVLFNENFRVFGGLLNGGGNNS-YEHVGVELVLDFFNMQNPSCVHDLETYI 428
           VNDP+TK+PGVLFNENFRV GG      + S Y HVGVEL LDFF++QN SCVHDL+TYI
Sbjct: 366 VNDPVTKLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYI 425

Query: 429 SLLRSPKEEEQEEENGRIIRKNNSGGDFINRAMEFFCSNAQSLNMFPRRNQ 461
            LL       Q   N R +  ++   D  N A+EF  +N + + MF +R +
Sbjct: 426 DLL------NQRRTNSRSV-DSDEDEDSDNVALEFLKTNGEKM-MFLKRQR 460

BLAST of MC05g0757 vs. ExPASy Swiss-Prot
Match: A2WY00 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=EG1 PE=3 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.3e-114
Identity = 209/359 (58.22%), Postives = 265/359 (73.82%), Query Frame = 0

Query: 65  VFSPATAAAVVHKVA-GLARLWRKIHGCDNWEDLVEPTLHPLLRREVIRYGEFVTVCYKA 124
           V +P        K A  +A +WR++ GC +WE ++EP  HP+LR EV RYGE V  CYKA
Sbjct: 39  VVAPVARRGAARKTAETVAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKA 98

Query: 125 FDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPPCCGRWI 184
           FDLDP S+RYL CK+G+  +L+EVG+  +GYEVT+YIYA   D+++P ++ S    GRWI
Sbjct: 99  FDLDPASRRYLNCKYGRERMLEEVGMGGAGYEVTRYIYAA-ADVSVPTMEPSTSGRGRWI 158

Query: 185 GYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPHNQRPDVKVESGF 244
           GYVAVS+DE S+ LGRRD++++FRGTVT +EW+ANLMSSL  ARLDP + RPDVKVESGF
Sbjct: 159 GYVAVSTDEMSRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGF 218

Query: 245 LTLYTSEETSIKF-GLESCREQLLSEVSRLLNKYK--NEEVSITMAGHSMGSALAVLLAY 304
           L+LYTS + + +F G  SCREQLL EVSRL+  Y    E+VS+T+AGHSMGSALA+L AY
Sbjct: 219 LSLYTSADKTCRFGGAGSCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAY 278

Query: 305 DIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPG 364
           D+AELGLNR        PV+VFSFGGPRVGN+ FK RC+ELGVK LR+ NV+DPITK+PG
Sbjct: 279 DLAELGLNR------AAPVTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPG 338

Query: 365 VLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLRSPKEEE 420
           V  NE      G+L    ++ Y HVGVEL LDFF + + + VHDL TYISLLR   +++
Sbjct: 339 VFLNE---ATAGVLRPWRHSCYTHVGVELPLDFFKVGDLASVHDLATYISLLRGADKKQ 387

BLAST of MC05g0757 vs. ExPASy Swiss-Prot
Match: Q8S1D9 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=EG1 PE=1 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.3e-114
Identity = 209/359 (58.22%), Postives = 265/359 (73.82%), Query Frame = 0

Query: 65  VFSPATAAAVVHKVA-GLARLWRKIHGCDNWEDLVEPTLHPLLRREVIRYGEFVTVCYKA 124
           V +P        K A  +A +WR++ GC +WE ++EP  HP+LR EV RYGE V  CYKA
Sbjct: 39  VVAPVARRGAARKTAETVAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKA 98

Query: 125 FDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPPCCGRWI 184
           FDLDP S+RYL CK+G+  +L+EVG+  +GYEVT+YIYA   D+++P ++ S    GRWI
Sbjct: 99  FDLDPASRRYLNCKYGRERMLEEVGMGGAGYEVTRYIYAA-ADVSVPTMEPSTSGRGRWI 158

Query: 185 GYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPHNQRPDVKVESGF 244
           GYVAVS+DE S+ LGRRD++++FRGTVT +EW+ANLMSSL  ARLDP + RPDVKVESGF
Sbjct: 159 GYVAVSTDEMSRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGF 218

Query: 245 LTLYTSEETSIKF-GLESCREQLLSEVSRLLNKYK--NEEVSITMAGHSMGSALAVLLAY 304
           L+LYTS + + +F G  SCREQLL EVSRL+  Y    E+VS+T+AGHSMGSALA+L AY
Sbjct: 219 LSLYTSADKTCRFGGAGSCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAY 278

Query: 305 DIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPG 364
           D+AELGLNR        PV+VFSFGGPRVGN+ FK RC+ELGVK LR+ NV+DPITK+PG
Sbjct: 279 DLAELGLNR------AAPVTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPG 338

Query: 365 VLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLRSPKEEE 420
           V  NE      G+L    ++ Y HVGVEL LDFF + + + VHDL TYISLLR   +++
Sbjct: 339 VFLNE---ATAGVLRPWRHSCYTHVGVELPLDFFKVGDLASVHDLATYISLLRGADKKQ 387

BLAST of MC05g0757 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 286.2 bits (731), Expect = 6.8e-76
Identity = 169/380 (44.47%), Postives = 225/380 (59.21%), Query Frame = 0

Query: 85  WRKIHGCDNWEDLVEPTLHPLLRREVIRYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLL 144
           WRKI G D+W  L++P + P+LR E+IRYGE    CY AFD DP S+   +C+F +R L 
Sbjct: 89  WRKIQGEDDWAGLMDP-MDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLF 148

Query: 145 KEVGLESSGYEVTKYIYATPPDINIPPIQNSPPCC------GRWIGYVAVSSDE--TSKS 204
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS D   T   
Sbjct: 149 DSLGIIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCR 208

Query: 205 LGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPHN-QRPDVKVESGFLTLYTSEETSIK 264
           LGRRDI I +RGTVT  EWIA+L   L P   +      P VK ESGFL LYT ++TS  
Sbjct: 209 LGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCN 268

Query: 265 FGLESCREQLLSEVSRLLNKY---KNEEVSITMAGHSMGSALAVLLAYDIAELGLNRRVN 324
           F   S REQ+L+EV RL+ +Y   + EE+SIT+ GHS+G ALAVL AYD+AE+G+NR   
Sbjct: 269 FSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRK 328

Query: 325 NKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGG 384
            K V+PV+ F++GGPRVGN  FK+R E+LGVKVLR+VN +D + K PG+  NE       
Sbjct: 329 GK-VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALM 388

Query: 385 LLNGGGNNSYEHVGVELVLDFFNMQNP--------SCVHDLETYISLLRSPKEEEQEEEN 444
            L GG    Y HVG  L LD  + ++P        S  H+LE  + LL     +    + 
Sbjct: 389 KLAGGLPWCYSHVGEMLPLD--HQKSPFLKPTVDLSTAHNLEALLHLL-----DGYHGKG 448

BLAST of MC05g0757 vs. NCBI nr
Match: XP_004148168.2 (galactolipase DONGLE, chloroplastic [Cucumis sativus] >KGN57254.1 hypothetical protein Csa_009777 [Cucumis sativus])

HSP 1 Score: 780 bits (2015), Expect = 1.13e-281
Identity = 411/496 (82.86%), Postives = 435/496 (87.70%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSS--------RQL 60
           MAA TLKLN IP+HVTF ET+RPMSHSFGQVS+P  RKSD  VSS SSS        R  
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIP--RKSDTNVSSSSSSSSSSSASRRLS 60

Query: 61  VSSSSVLVPLIDVFSP--------ATAAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHP 120
           VSSSSVLVPL+DVFS         A AAA+V KVA LARLWR+IHGC++WEDLVEPTLHP
Sbjct: 61  VSSSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHP 120

Query: 121 LLRREVIRYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIYATP 180
           LLRRE+IRYGEFVT CYKAFDLDPNSKRYLTCKFGK+SLLKEVGLESSGYEVTKYIYATP
Sbjct: 121 LLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATP 180

Query: 181 PDINIPPIQNSPPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLT 240
           PDINIPPIQNSPP CGRWIGYVAVSSDETSK LGRRDI+ITFRGTVTN EWIANLMSSLT
Sbjct: 181 PDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLT 240

Query: 241 PARLDPHNQRPDVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSIT 300
           PARLDPHN RPDVKVESGFLTLYTSEE+S+KFGLESCREQLLSEVSRLLNKYK EEVSIT
Sbjct: 241 PARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSIT 300

Query: 301 MAGHSMGSALAVLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVK 360
           MAGHSMGSALA+LLAYDIAELGLNRR NN EVVPVSVFSFGGPRVGNSGFK+RCEELGVK
Sbjct: 301 MAGHSMGSALALLLAYDIAELGLNRRTNN-EVVPVSVFSFGGPRVGNSGFKKRCEELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFRVFGGLLNGG NNSYEHVGVELVLDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRSPKEEEQEEEN-------GRIIRKNNSGGDFINRAMEFFCSNAQSLNMFPR 473
           LETYISLLR PK++E E+E+       G+II    + G+FIN+AMEF CSNAQSLNMFP 
Sbjct: 421 LETYISLLRCPKKQEDEDEDEDEDSGRGKIII---NSGEFINKAMEFLCSNAQSLNMFPW 480

BLAST of MC05g0757 vs. NCBI nr
Match: XP_038886126.1 (galactolipase DONGLE, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 765 bits (1976), Expect = 7.24e-276
Identity = 402/485 (82.89%), Postives = 428/485 (88.25%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSSRQL-VSSSSVL 60
           MAA TLKLN IP+HVTF ET+RPMSHSFGQV +P+  K +NV+S  SSSR+L VSSS VL
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVLIPI--KPNNVLSLSSSSRRLSVSSSLVL 60

Query: 61  VPLIDVFSP----ATAAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHPLLRREVIRYGE 120
           VPL+DVFS     A AAA+VHKVA LA LWR+IHGC++WEDL+EPTLHPLLRRE+IRYGE
Sbjct: 61  VPLMDVFSSSCSTAAAAAMVHKVASLAHLWRQIHGCNDWEDLMEPTLHPLLRREIIRYGE 120

Query: 121 FVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNS 180
           FVT CYKAFDLDPNSKRYLTCKFGK++LL+EVGLESSGYEVTKYIYATPPDINI PIQNS
Sbjct: 121 FVTACYKAFDLDPNSKRYLTCKFGKKNLLREVGLESSGYEVTKYIYATPPDINISPIQNS 180

Query: 181 PPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPHNQRP 240
           PP CGRWIGYVAVSSDETSK LGRRDIVITFRGTVTN EWIANLMSSLT ARLDPHN RP
Sbjct: 181 PPSCGRWIGYVAVSSDETSKRLGRRDIVITFRGTVTNPEWIANLMSSLTLARLDPHNHRP 240

Query: 241 DVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALA 300
           DVKVESGFLTLYTSEETS+KFGLESCREQLLSEVSRLLNKYK EEVSITMAGHSMGSALA
Sbjct: 241 DVKVESGFLTLYTSEETSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMAGHSMGSALA 300

Query: 301 VLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPI 360
           +LLAYDIAELGLNRR NN EVVPVSVFSFGGPRVGNSGFK+RCEELGVKVLRIVNVNDPI
Sbjct: 301 LLLAYDIAELGLNRRTNN-EVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLRIVNVNDPI 360

Query: 361 TKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLR-S 420
           TKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLR  
Sbjct: 361 TKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLRCD 420

Query: 421 PKEEEQEEENGRIIRKNN------SGGDFINRAMEFFCSNAQSLNMFPRRNQGSNRVRYL 473
           PK+EE  ++N      N       + G+FIN+AMEF CSNAQ LNMFP R      V YL
Sbjct: 421 PKQEEDHQDNDDDDHNNGRRKIIINSGEFINKAMEFLCSNAQILNMFPWRIP----VNYL 478

BLAST of MC05g0757 vs. NCBI nr
Match: XP_008439078.1 (PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis melo] >KAA0067660.1 galactolipase DONGLE [Cucumis melo var. makuwa] >TYK23664.1 galactolipase DONGLE [Cucumis melo var. makuwa])

HSP 1 Score: 765 bits (1976), Expect = 1.22e-275
Identity = 407/500 (81.40%), Postives = 433/500 (86.60%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSS---------RQ 60
           MAA TLKLN IP+HVTF ET+RPMSHSFGQVS+P  RKSD  VSS SSS         R 
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIP--RKSDINVSSSSSSSSYSSSASRRL 60

Query: 61  LVSSSSVLVPLIDVFSP----------ATAAAVVHKVAGLARLWRKIHGCDNWEDLVEPT 120
            VSSSSVLVPL+DVFS           A AAA+V KVA LARLWR+IHGC++WEDLVEPT
Sbjct: 61  SVSSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPT 120

Query: 121 LHPLLRREVIRYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIY 180
           LHPLLRRE+IRYGEFVT CYKAFDLDPNSKRYLTCKFGK+SLLKEVGLESSGYEVTKYIY
Sbjct: 121 LHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIY 180

Query: 181 ATPPDINIPPIQNSPPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMS 240
           ATPPDINIPPIQNSPP CGRWIGYVAVSSDETSK LGRRDI+ITFRGTVT+ EWIANLMS
Sbjct: 181 ATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMS 240

Query: 241 SLTPARLDPHNQRPDVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEV 300
           SLTPARLDPHN RPDVKVESGFLTLYTSEE+++KFGLESCREQLLSEVSR+LNKYK EEV
Sbjct: 241 SLTPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEV 300

Query: 301 SITMAGHSMGSALAVLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEEL 360
           SITMAGHSMGSALA+LLAYDIAELGLNRR NN EVVPVSVFSFGGPRVGNSGFK+RC+EL
Sbjct: 301 SITMAGHSMGSALALLLAYDIAELGLNRRTNN-EVVPVSVFSFGGPRVGNSGFKKRCDEL 360

Query: 361 GVKVLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSC 420
           GVKVLRIVNVNDPITKMPGVLFNENFR FGGLLNGG NNSYEHVGVELVLDFFNMQNPSC
Sbjct: 361 GVKVLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSC 420

Query: 421 VHDLETYISLLRSPKEEE---QEEEN-----GRIIRKNNSGGDFINRAMEFFCSNAQSLN 473
           VHDLETYISLLR PK++E   Q+E+N     GR     NSG +FIN+A EF CSNAQSLN
Sbjct: 421 VHDLETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSG-EFINKAKEFLCSNAQSLN 480

BLAST of MC05g0757 vs. NCBI nr
Match: XP_022956251.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 709 bits (1830), Expect = 5.78e-254
Identity = 384/489 (78.53%), Postives = 408/489 (83.44%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSSRQLVSSSSVLV 60
           MA  TLKLN   +HVTFSET+RP+SHSFGQVSLP  RKSD+V S+   S    SSSSVLV
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLP--RKSDHVSSTSRRSVSSSSSSSVLV 60

Query: 61  PLIDVFSPAT---------AAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHPLLRREVI 120
           PLID+FS ++         A A+VHKVAGLARLWR+IHGC++WE LVEPTLHPLLRREVI
Sbjct: 61  PLIDMFSSSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVI 120

Query: 121 RYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIYATPPDINIPP 180
           RYGEFVT CYKAFDLDPNSKRYLTCKFGK+SLLKEVGLESSGYEVTKYIYATPPDINIPP
Sbjct: 121 RYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPP 180

Query: 181 IQNSPPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPH 240
           IQNSPP CGRWIGYVAVSSDETS+ LGRRD+VITFRGTVTNSEWIANLMSSLTPARLDPH
Sbjct: 181 IQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPH 240

Query: 241 NQRPDVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMG 300
           N RPDVKVESGFLTLYT EETS KFGLESCREQLLSE+SRLLNKYK+EEVSITMAGHSMG
Sbjct: 241 NHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMG 300

Query: 301 SALAVLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNV 360
           SALA+LLAYDIAELGLN+R N KEVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR+VNV
Sbjct: 301 SALALLLAYDIAELGLNKRTN-KEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNV 360

Query: 361 NDPITKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISL 420
           NDPITKMPGV        FGG         YEHVGVELVLDFFNMQNPSCVHDLETYISL
Sbjct: 361 NDPITKMPGV--------FGG---------YEHVGVELVLDFFNMQNPSCVHDLETYISL 420

Query: 421 LRSPKEEEQEEE-------NGRIIRKNNSGGDFINRAMEFFCSNAQSLNMFPRRNQGSNR 473
           LR PKEE QE +       NGR  RK  +GGDFIN+AMEF      S NMFP RN     
Sbjct: 421 LRYPKEEHQEHDDDDHHHHNGR--RKAINGGDFINKAMEFL-----SFNMFPWRNP---- 458

BLAST of MC05g0757 vs. NCBI nr
Match: KAG6582093.1 (Galactolipase DONGLE, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 708 bits (1827), Expect = 1.37e-253
Identity = 383/484 (79.13%), Postives = 407/484 (84.09%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSSRQLVSSSSVLV 60
           MA  TLKLN   +HVTFSET+RP+SHSFGQVSLP  RKSD+V S+   S    SSSSVLV
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLP--RKSDHVSSTSRRSVSSSSSSSVLV 60

Query: 61  PLIDVFSPAT-----AAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHPLLRREVIRYGE 120
           PLID+ S ++     A A+VHKVAGLARLWR+IHGC++WE LVEPTLHPLLRREVIRYGE
Sbjct: 61  PLIDMSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGE 120

Query: 121 FVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNS 180
           FVT CYKAFDLDPNSKRYLTCKFGK+SLLKEVGLESSGYEVTKYIYATPPDINIPPIQNS
Sbjct: 121 FVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNS 180

Query: 181 PPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPHNQRP 240
           PP CGRWIGYVAVSSDETS+ LGRRD+VITFRGTVTNSEWIANLMSSLTPARLDPHN RP
Sbjct: 181 PPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRP 240

Query: 241 DVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALA 300
           DVKVESGFLTLYT EETS KFGLESCREQLLSE+SRLLNKYK+EEVSITMAGHSMGSALA
Sbjct: 241 DVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALA 300

Query: 301 VLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPI 360
           +LLAYDIAELGLN+R N KEVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR+VNVNDPI
Sbjct: 301 LLLAYDIAELGLNKRTN-KEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPI 360

Query: 361 TKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLRSP 420
           TKMPGV        FGG         YEHVGVELVLDFFNMQNPSCVHDLETYISLLR P
Sbjct: 361 TKMPGV--------FGG---------YEHVGVELVLDFFNMQNPSCVHDLETYISLLRYP 420

Query: 421 KEEEQEEE------NGRIIRKNNSGGDFINRAMEFFCSNAQSLNMFPRRNQGSNRVRYLS 473
           KEE QE +      NGR  RK  +GGDFIN+AMEF      S NMFP RN     + YLS
Sbjct: 421 KEEHQEHDDDHHHHNGR--RKAINGGDFINKAMEFL-----SFNMFPWRNP----MNYLS 453

BLAST of MC05g0757 vs. ExPASy TrEMBL
Match: A0A0A0L8A7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174570 PE=4 SV=1)

HSP 1 Score: 780 bits (2015), Expect = 5.45e-282
Identity = 411/496 (82.86%), Postives = 435/496 (87.70%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSS--------RQL 60
           MAA TLKLN IP+HVTF ET+RPMSHSFGQVS+P  RKSD  VSS SSS        R  
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIP--RKSDTNVSSSSSSSSSSSASRRLS 60

Query: 61  VSSSSVLVPLIDVFSP--------ATAAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHP 120
           VSSSSVLVPL+DVFS         A AAA+V KVA LARLWR+IHGC++WEDLVEPTLHP
Sbjct: 61  VSSSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHP 120

Query: 121 LLRREVIRYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIYATP 180
           LLRRE+IRYGEFVT CYKAFDLDPNSKRYLTCKFGK+SLLKEVGLESSGYEVTKYIYATP
Sbjct: 121 LLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATP 180

Query: 181 PDINIPPIQNSPPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLT 240
           PDINIPPIQNSPP CGRWIGYVAVSSDETSK LGRRDI+ITFRGTVTN EWIANLMSSLT
Sbjct: 181 PDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLT 240

Query: 241 PARLDPHNQRPDVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSIT 300
           PARLDPHN RPDVKVESGFLTLYTSEE+S+KFGLESCREQLLSEVSRLLNKYK EEVSIT
Sbjct: 241 PARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSIT 300

Query: 301 MAGHSMGSALAVLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVK 360
           MAGHSMGSALA+LLAYDIAELGLNRR NN EVVPVSVFSFGGPRVGNSGFK+RCEELGVK
Sbjct: 301 MAGHSMGSALALLLAYDIAELGLNRRTNN-EVVPVSVFSFGGPRVGNSGFKKRCEELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFRVFGGLLNGG NNSYEHVGVELVLDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRSPKEEEQEEEN-------GRIIRKNNSGGDFINRAMEFFCSNAQSLNMFPR 473
           LETYISLLR PK++E E+E+       G+II    + G+FIN+AMEF CSNAQSLNMFP 
Sbjct: 421 LETYISLLRCPKKQEDEDEDEDEDSGRGKIII---NSGEFINKAMEFLCSNAQSLNMFPW 480

BLAST of MC05g0757 vs. ExPASy TrEMBL
Match: A0A5A7VKE2 (Galactolipase DONGLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00300 PE=4 SV=1)

HSP 1 Score: 765 bits (1976), Expect = 5.90e-276
Identity = 407/500 (81.40%), Postives = 433/500 (86.60%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSS---------RQ 60
           MAA TLKLN IP+HVTF ET+RPMSHSFGQVS+P  RKSD  VSS SSS         R 
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIP--RKSDINVSSSSSSSSYSSSASRRL 60

Query: 61  LVSSSSVLVPLIDVFSP----------ATAAAVVHKVAGLARLWRKIHGCDNWEDLVEPT 120
            VSSSSVLVPL+DVFS           A AAA+V KVA LARLWR+IHGC++WEDLVEPT
Sbjct: 61  SVSSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPT 120

Query: 121 LHPLLRREVIRYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIY 180
           LHPLLRRE+IRYGEFVT CYKAFDLDPNSKRYLTCKFGK+SLLKEVGLESSGYEVTKYIY
Sbjct: 121 LHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIY 180

Query: 181 ATPPDINIPPIQNSPPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMS 240
           ATPPDINIPPIQNSPP CGRWIGYVAVSSDETSK LGRRDI+ITFRGTVT+ EWIANLMS
Sbjct: 181 ATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMS 240

Query: 241 SLTPARLDPHNQRPDVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEV 300
           SLTPARLDPHN RPDVKVESGFLTLYTSEE+++KFGLESCREQLLSEVSR+LNKYK EEV
Sbjct: 241 SLTPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEV 300

Query: 301 SITMAGHSMGSALAVLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEEL 360
           SITMAGHSMGSALA+LLAYDIAELGLNRR NN EVVPVSVFSFGGPRVGNSGFK+RC+EL
Sbjct: 301 SITMAGHSMGSALALLLAYDIAELGLNRRTNN-EVVPVSVFSFGGPRVGNSGFKKRCDEL 360

Query: 361 GVKVLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSC 420
           GVKVLRIVNVNDPITKMPGVLFNENFR FGGLLNGG NNSYEHVGVELVLDFFNMQNPSC
Sbjct: 361 GVKVLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSC 420

Query: 421 VHDLETYISLLRSPKEEE---QEEEN-----GRIIRKNNSGGDFINRAMEFFCSNAQSLN 473
           VHDLETYISLLR PK++E   Q+E+N     GR     NSG +FIN+A EF CSNAQSLN
Sbjct: 421 VHDLETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSG-EFINKAKEFLCSNAQSLN 480

BLAST of MC05g0757 vs. ExPASy TrEMBL
Match: A0A1S3AXV1 (galactolipase DONGLE, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483973 PE=4 SV=1)

HSP 1 Score: 765 bits (1976), Expect = 5.90e-276
Identity = 407/500 (81.40%), Postives = 433/500 (86.60%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSS---------RQ 60
           MAA TLKLN IP+HVTF ET+RPMSHSFGQVS+P  RKSD  VSS SSS         R 
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIP--RKSDINVSSSSSSSSYSSSASRRL 60

Query: 61  LVSSSSVLVPLIDVFSP----------ATAAAVVHKVAGLARLWRKIHGCDNWEDLVEPT 120
            VSSSSVLVPL+DVFS           A AAA+V KVA LARLWR+IHGC++WEDLVEPT
Sbjct: 61  SVSSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPT 120

Query: 121 LHPLLRREVIRYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIY 180
           LHPLLRRE+IRYGEFVT CYKAFDLDPNSKRYLTCKFGK+SLLKEVGLESSGYEVTKYIY
Sbjct: 121 LHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIY 180

Query: 181 ATPPDINIPPIQNSPPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMS 240
           ATPPDINIPPIQNSPP CGRWIGYVAVSSDETSK LGRRDI+ITFRGTVT+ EWIANLMS
Sbjct: 181 ATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMS 240

Query: 241 SLTPARLDPHNQRPDVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEV 300
           SLTPARLDPHN RPDVKVESGFLTLYTSEE+++KFGLESCREQLLSEVSR+LNKYK EEV
Sbjct: 241 SLTPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEV 300

Query: 301 SITMAGHSMGSALAVLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEEL 360
           SITMAGHSMGSALA+LLAYDIAELGLNRR NN EVVPVSVFSFGGPRVGNSGFK+RC+EL
Sbjct: 301 SITMAGHSMGSALALLLAYDIAELGLNRRTNN-EVVPVSVFSFGGPRVGNSGFKKRCDEL 360

Query: 361 GVKVLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSC 420
           GVKVLRIVNVNDPITKMPGVLFNENFR FGGLLNGG NNSYEHVGVELVLDFFNMQNPSC
Sbjct: 361 GVKVLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSC 420

Query: 421 VHDLETYISLLRSPKEEE---QEEEN-----GRIIRKNNSGGDFINRAMEFFCSNAQSLN 473
           VHDLETYISLLR PK++E   Q+E+N     GR     NSG +FIN+A EF CSNAQSLN
Sbjct: 421 VHDLETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSG-EFINKAKEFLCSNAQSLN 480

BLAST of MC05g0757 vs. ExPASy TrEMBL
Match: A0A6J1GW19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458003 PE=4 SV=1)

HSP 1 Score: 709 bits (1830), Expect = 2.80e-254
Identity = 384/489 (78.53%), Postives = 408/489 (83.44%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSSRQLVSSSSVLV 60
           MA  TLKLN   +HVTFSET+RP+SHSFGQVSLP  RKSD+V S+   S    SSSSVLV
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLP--RKSDHVSSTSRRSVSSSSSSSVLV 60

Query: 61  PLIDVFSPAT---------AAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHPLLRREVI 120
           PLID+FS ++         A A+VHKVAGLARLWR+IHGC++WE LVEPTLHPLLRREVI
Sbjct: 61  PLIDMFSSSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVI 120

Query: 121 RYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIYATPPDINIPP 180
           RYGEFVT CYKAFDLDPNSKRYLTCKFGK+SLLKEVGLESSGYEVTKYIYATPPDINIPP
Sbjct: 121 RYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPP 180

Query: 181 IQNSPPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPH 240
           IQNSPP CGRWIGYVAVSSDETS+ LGRRD+VITFRGTVTNSEWIANLMSSLTPARLDPH
Sbjct: 181 IQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPH 240

Query: 241 NQRPDVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMG 300
           N RPDVKVESGFLTLYT EETS KFGLESCREQLLSE+SRLLNKYK+EEVSITMAGHSMG
Sbjct: 241 NHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMG 300

Query: 301 SALAVLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNV 360
           SALA+LLAYDIAELGLN+R N KEVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR+VNV
Sbjct: 301 SALALLLAYDIAELGLNKRTN-KEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNV 360

Query: 361 NDPITKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISL 420
           NDPITKMPGV        FGG         YEHVGVELVLDFFNMQNPSCVHDLETYISL
Sbjct: 361 NDPITKMPGV--------FGG---------YEHVGVELVLDFFNMQNPSCVHDLETYISL 420

Query: 421 LRSPKEEEQEEE-------NGRIIRKNNSGGDFINRAMEFFCSNAQSLNMFPRRNQGSNR 473
           LR PKEE QE +       NGR  RK  +GGDFIN+AMEF      S NMFP RN     
Sbjct: 421 LRYPKEEHQEHDDDDHHHHNGR--RKAINGGDFINKAMEFL-----SFNMFPWRNP---- 458

BLAST of MC05g0757 vs. ExPASy TrEMBL
Match: A0A6J1IP19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479244 PE=4 SV=1)

HSP 1 Score: 707 bits (1826), Expect = 9.10e-254
Identity = 382/483 (79.09%), Postives = 407/483 (84.27%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSSRQLVSSSSVLV 60
           MA  TLKLN   +HVTFSET+RPMSHSFGQVSLP  RKSD+V S+   S    SSSSVLV
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPMSHSFGQVSLP--RKSDHVSSTSRRSVSSSSSSSVLV 60

Query: 61  PLIDVFSPAT-----AAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHPLLRREVIRYGE 120
           PLID+FS ++     A A+VHK+AGLARLWR+IHGC++WE LVEPTLHPLLRREVIRYGE
Sbjct: 61  PLIDMFSSSSSSASAAVAMVHKMAGLARLWRQIHGCNDWEHLVEPTLHPLLRREVIRYGE 120

Query: 121 FVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNS 180
           FVT CYKAFDLDPNSKRYLTCKFGK+SLLKEVGLESSGYEVTKYIYATPPDINIPPIQNS
Sbjct: 121 FVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNS 180

Query: 181 PPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPHNQRP 240
           PP CGRWIGYVAVSSDETSK LGRRD+VITFRGTVTNSEWIANLMSSLTPARLDPHN RP
Sbjct: 181 PPSCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPARLDPHNHRP 240

Query: 241 DVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALA 300
           DVKVESGFLTLYT EETS KFGLESCREQLLSE+SRLLNKYK+EEVSITMAGHSMGSALA
Sbjct: 241 DVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMAGHSMGSALA 300

Query: 301 VLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPI 360
           +LLAYDIAELGLN+R N KEVVPVSVFSFGGPRVGNS FKQRCEELGVKVLR+VNVNDPI
Sbjct: 301 LLLAYDIAELGLNKRTN-KEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLRVVNVNDPI 360

Query: 361 TKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLRSP 420
           TK+PGV        FGG         YEHVGVELVLDFFNMQNPSCVHDLETYISLLR P
Sbjct: 361 TKIPGV--------FGG---------YEHVGVELVLDFFNMQNPSCVHDLETYISLLRYP 420

Query: 421 KEEEQEEE-----NGRIIRKNNSGGDFINRAMEFFCSNAQSLNMFPRRNQGSNRVRYLSQ 473
           KEE Q+ +     NGR  RK  +GGDFIN+AMEF      S NM P RN     + YLSQ
Sbjct: 421 KEEHQKHDDDHHHNGR--RKVINGGDFINKAMEFL-----SFNMLPWRNP----MNYLSQ 452

BLAST of MC05g0757 vs. TAIR 10
Match: AT1G05800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 494.2 bits (1271), Expect = 1.2e-139
Identity = 266/455 (58.46%), Postives = 329/455 (72.31%), Query Frame = 0

Query: 1   MAAITLKLNPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSSRQLVSSSSVLV 60
           MAA     NPI       + +    H+    S+     S  +V S S+    +SSS + +
Sbjct: 1   MAAKVFTQNPIYSQSLVRDKTPQQKHNLDHFSIS-QHTSKRLVVSSSTMSPPISSSPLSL 60

Query: 61  P---LIDVFSPATAAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHPLLRREVIRYGEFV 120
           P         P+ A AV      L+R+WR+I G +NWE+L+EP L P+L++E+ RYG  +
Sbjct: 61  PSSSSSQAIPPSRAPAV---TLPLSRVWREIQGSNNWENLIEP-LSPILQQEITRYGNLL 120

Query: 121 TVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGL-ESSGYEVTKYIYATPPDINIPPIQNSP 180
           +  YK FDL+PNSKRYL+CK+GK++LLKE G+ +  GY+VTKYIYAT PDIN+ PI+N P
Sbjct: 121 SASYKGFDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYIYAT-PDINLNPIKNEP 180

Query: 181 PCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPHNQRPD 240
               RWIGYVAVSSDE+ K LGRRDI++TFRGTVTN EW+ANL SSLTPARLDPHN RPD
Sbjct: 181 N-RARWIGYVAVSSDESVKRLGRRDILVTFRGTVTNHEWLANLKSSLTPARLDPHNPRPD 240

Query: 241 VKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSMGSALAV 300
           VKVESGFL LYTS E+  KFGLESCREQLLSE+SRL+NK+K EE+SIT+AGHSMGS+LA 
Sbjct: 241 VKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKHKGEEISITLAGHSMGSSLAQ 300

Query: 301 LLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPIT 360
           LLAYDIAELG+N+R + K  VPV+VFSF GPRVGN GFK+RCEELGVKVLRI NVNDPIT
Sbjct: 301 LLAYDIAELGMNQRRDEKP-VPVTVFSFAGPRVGNLGFKKRCEELGVKVLRITNVNDPIT 360

Query: 361 KMPGVLFNENFRVFGGLLNGGGNNS-YEHVGVELVLDFFNMQNPSCVHDLETYISLLRSP 420
           K+PG LFNENFR  GG+     + S Y HVGVEL LDFF++QN SCVHDLETYI+L+  P
Sbjct: 361 KLPGFLFNENFRSLGGVYELPWSCSCYTHVGVELTLDFFDVQNISCVHDLETYITLVNRP 420

Query: 421 KEEEQEEENGRIIRKNNSGGDFINRAMEFFCSNAQ 451
           +  +        + ++N GG+F+NR  E   S  +
Sbjct: 421 RCSK------LAVNEDNFGGEFLNRTSELMFSKGR 441

BLAST of MC05g0757 vs. TAIR 10
Match: AT2G31690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 490.0 bits (1260), Expect = 2.2e-138
Identity = 270/471 (57.32%), Postives = 329/471 (69.85%), Query Frame = 0

Query: 9   NPIPHHVTFSETSRPMSHSFGQVSLPLPRKSDNVVSSYSSSRQLVSSSSVLVPLI----- 68
           NP   H  F     P    F   +L LP    N     +++    SSSS+L P+I     
Sbjct: 6   NPNMKHAPFLRNRSPQQTLFIPYTLSLPISYQNPKRLKTANSS--SSSSLLAPVILNSPV 65

Query: 69  -----------DVFSPATAAAVVHKVAGLARLWRKIHGCDNWEDLVEPTLHPLLRREVIR 128
                        F  ++ AA V     L+R+WR+I GC+NW+DL+EP L+PLL++E+ R
Sbjct: 66  ASSSPPPIYCAPKFPCSSGAATV----PLSRVWREIQGCNNWKDLIEP-LNPLLQQEITR 125

Query: 129 YGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLLKEVGLES-SGYEVTKYIYATPP-DINIP 188
           YG  V+ CYKAFDLDPNSKRYL CK+GK++LLKE  ++    Y+VTKYIYATP  +INI 
Sbjct: 126 YGNLVSTCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQVTKYIYATPDININIS 185

Query: 189 PIQNSPPCCGRWIGYVAVSSDETSKSLGRRDIVITFRGTVTNSEWIANLMSSLTPARLDP 248
           PIQN      RW+GYVA SSD++ K LGRRDIV+TFRGTVTN EW+AN MSSLTPAR  P
Sbjct: 186 PIQNEMNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEWLANFMSSLTPARFHP 245

Query: 249 HNQRPDVKVESGFLTLYTSEETSIKFGLESCREQLLSEVSRLLNKYKNEEVSITMAGHSM 308
           HN R DVKVESGFL+LYTS+E+  KFGLESCR+QLLSE+SRL+NKYK EE+SIT+AGHSM
Sbjct: 246 HNPRLDVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNKYKGEEMSITLAGHSM 305

Query: 309 GSALAVLLAYDIAELGLNRRVNNKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVN 368
           GS+LA LLAYDIAELGLNRR+  K  +PV+VFSF GPRVGN  FK+RCEELGVKVLRI N
Sbjct: 306 GSSLAQLLAYDIAELGLNRRI-GKGDIPVTVFSFAGPRVGNLEFKKRCEELGVKVLRITN 365

Query: 369 VNDPITKMPGVLFNENFRVFGGLLNGGGNNS-YEHVGVELVLDFFNMQNPSCVHDLETYI 428
           VNDP+TK+PGVLFNENFRV GG      + S Y HVGVEL LDFF++QN SCVHDL+TYI
Sbjct: 366 VNDPVTKLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFDVQNISCVHDLQTYI 425

Query: 429 SLLRSPKEEEQEEENGRIIRKNNSGGDFINRAMEFFCSNAQSLNMFPRRNQ 461
            LL       Q   N R +  ++   D  N A+EF  +N + + MF +R +
Sbjct: 426 DLL------NQRRTNSRSV-DSDEDEDSDNVALEFLKTNGEKM-MFLKRQR 460

BLAST of MC05g0757 vs. TAIR 10
Match: AT1G06800.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 2.8e-77
Identity = 165/348 (47.41%), Postives = 214/348 (61.49%), Query Frame = 0

Query: 85  WRKIHGCDNWEDLVEPTLHPLLRREVIRYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLL 144
           WRKI G D+W  L++P + P+LR E+IRYGE    CY AFD DP S+   +C+F +R L 
Sbjct: 89  WRKIQGEDDWAGLMDP-MDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLF 148

Query: 145 KEVGLESSGYEVTKYIYATPPDINIPPIQNSPPCC------GRWIGYVAVSSDE--TSKS 204
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS D   T   
Sbjct: 149 DSLGIIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCR 208

Query: 205 LGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPHN-QRPDVKVESGFLTLYTSEETSIK 264
           LGRRDI I +RGTVT  EWIA+L   L P   +      P VK ESGFL LYT ++TS  
Sbjct: 209 LGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCN 268

Query: 265 FGLESCREQLLSEVSRLLNKY---KNEEVSITMAGHSMGSALAVLLAYDIAELGLNRRVN 324
           F   S REQ+L+EV RL+ +Y   + EE+SIT+ GHS+G ALAVL AYD+AE+G+NR   
Sbjct: 269 FSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRK 328

Query: 325 NKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGG 384
            K V+PV+ F++GGPRVGN  FK+R E+LGVKVLR+VN +D + K PG+  NE       
Sbjct: 329 GK-VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALM 388

Query: 385 LLNGGGNNSYEHVGVELVLDFFNMQNP--------SCVHDLETYISLL 413
            L GG    Y HVG  L LD  + ++P        S  H+LE  + LL
Sbjct: 389 KLAGGLPWCYSHVGEMLPLD--HQKSPFLKPTVDLSTAHNLEALLHLL 431

BLAST of MC05g0757 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 2.8e-77
Identity = 163/345 (47.25%), Postives = 216/345 (62.61%), Query Frame = 0

Query: 85  WRKIHGCDNWEDLVEPTLHPLLRREVIRYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLL 144
           WRKI G D+W  L++P + P+LR E+IRYGE    CY AFD DP SK   T +F +    
Sbjct: 105 WRKIQGEDDWAGLMDP-MDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFF 164

Query: 145 KEVGLESSGYEVTKYIYATPPDINIPPIQNSPPCC------GRWIGYVAVSSDETSKS-L 204
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS DETS++ L
Sbjct: 165 DSLGMIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRL 224

Query: 205 GRRDIVITFRGTVTNSEWIANLMSSLTPARLDP-HNQRPDVKVESGFLTLYTSEETSIKF 264
           GRRDI I +RGTVT  EWIA+L   L P   +      P VKVESGFL LYT ++T+ KF
Sbjct: 225 GRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKF 284

Query: 265 GLESCREQLLSEVSRLLNKYKNE---EVSITMAGHSMGSALAVLLAYDIAELGLNRRVNN 324
              S REQ+L+EV RL+ ++ ++   ++SIT+ GHS+G ALA+L AYDIAE+ LNR    
Sbjct: 285 ARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKG 344

Query: 325 KEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGGL 384
           K V+PV+V ++GGPRVGN  F++R EELGVKV+R+VNV+D + K PG+  NE+       
Sbjct: 345 K-VIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMK 404

Query: 385 LNGGGNNSYEHVGVELVLDFFNMQ------NPSCVHDLETYISLL 413
           +  G    Y HVG EL LD  N        + S  H+LE  + LL
Sbjct: 405 IAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLL 446

BLAST of MC05g0757 vs. TAIR 10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 4.8e-77
Identity = 169/380 (44.47%), Postives = 225/380 (59.21%), Query Frame = 0

Query: 85  WRKIHGCDNWEDLVEPTLHPLLRREVIRYGEFVTVCYKAFDLDPNSKRYLTCKFGKRSLL 144
           WRKI G D+W  L++P + P+LR E+IRYGE    CY AFD DP S+   +C+F +R L 
Sbjct: 89  WRKIQGEDDWAGLMDP-MDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLF 148

Query: 145 KEVGLESSGYEVTKYIYATPPDINIPPIQNSPPCC------GRWIGYVAVSSDE--TSKS 204
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS D   T   
Sbjct: 149 DSLGIIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCR 208

Query: 205 LGRRDIVITFRGTVTNSEWIANLMSSLTPARLDPHN-QRPDVKVESGFLTLYTSEETSIK 264
           LGRRDI I +RGTVT  EWIA+L   L P   +      P VK ESGFL LYT ++TS  
Sbjct: 209 LGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCN 268

Query: 265 FGLESCREQLLSEVSRLLNKY---KNEEVSITMAGHSMGSALAVLLAYDIAELGLNRRVN 324
           F   S REQ+L+EV RL+ +Y   + EE+SIT+ GHS+G ALAVL AYD+AE+G+NR   
Sbjct: 269 FSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRK 328

Query: 325 NKEVVPVSVFSFGGPRVGNSGFKQRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGG 384
            K V+PV+ F++GGPRVGN  FK+R E+LGVKVLR+VN +D + K PG+  NE       
Sbjct: 329 GK-VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALM 388

Query: 385 LLNGGGNNSYEHVGVELVLDFFNMQNP--------SCVHDLETYISLLRSPKEEEQEEEN 444
            L GG    Y HVG  L LD  + ++P        S  H+LE  + LL     +    + 
Sbjct: 389 KLAGGLPWCYSHVGEMLPLD--HQKSPFLKPTVDLSTAHNLEALLHLL-----DGYHGKG 448