MC05g0726 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0726
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionhistidine protein methyltransferase 1 homolog
LocationMC05: 5751245 .. 5756743 (+)
RNA-Seq ExpressionMC05g0726
SyntenyMC05g0726
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCAACATAATCTTCCACTGCAGTTACTCCATCCGACACATATATTTCGTGGATTTCCCTACCTTCACACAACGTCAACAGCCCGTCAAGAGAATTTTCACTACTTTTTCTGCTTCAATGCCAAAGTACGATGTCAATGTGGTAGGTTCTTTCCATGGGTTGCTATTTTTATCTGATTTTCTTTACAATGATAAGCTCTTTATATACATCCCATTTATAAGAGACTACCTGGAGTTGCCTAGAACCAATGAATTTTTTGACCTTGATGTGGTGTGTGGGATTTAATTTCACCCACAAATAAACGTGGTTAAAATTATGTTCTCAAAGATTTCATTGGATTAAAAGATACTCTTCTCCCCACCAGTCGGAGGTCTAAACCTTCACTCTGAGAACCTCCAATTGGAGGAGCCTACGCAAAATTTGCCACCGTCTGGCTCAGGGCAGTCTCTACTAAGTATCTCTACCTCCACTGCTACTTCATGTTGTTCGACTTGGCCGACTAACAATTCCAAGAAATCTCGAAGCCGGAATTAGGGAGCTTGAGCAGATGCAATTACCATTGATTCTTGATGGGTGCCTCTCTGCTACGGCCTACTGCAACTATGTGGACATGGAGATATGAGTGATGAAAGAGCAAGGGTCGACGAATCTTGGGTTTGGGTGAAGGAAGAAAATTTTCTTTTTATAATTTTTGTTTTAATTTTAAATCAGTCTAAATTAAATCAACCCGAACAACCCACTTAGTTGACTATTTAATTTGTACGCCACATTAGATTTAAGGGTAAACCAATTTTAAAGTAAAATGATTAAATCGTTATTTTTTAAAGTTTAAAGATTAAATCGTTAATTTAATAAAAGTTATTTACAATTTTAGAAAGAAAAAAAAAAAAAACCTTGGACTCAGAAGGTTTGGAGGCGGAGCTCGAACGCGAAAGCTCTGTTCCGCCGTTGAGAAGGAGGAACTCTCAGGGGAGCAACCAGCGATGGCGGCGAACTCTTCGGTAACCTCAAATTGCTGTATTCCTCTCTAAGAATCTCCTTACTCTAAACATCTCACTTCTTACACCTTTGATGATTTGAATCTCTTCTATTGAATTTCTTTCGCCATTTATGGAATTCCAGAGTCCGGCTTTCGAAAATTTCCGGTTGTTTGCGTCAGTGGAAGACGAGAAGTCCAATCCAGGTCTCGGTATAGGTTTTCTCGACTCTCAACAGCCTTCTCTTCCACCTCCACCTCCTTGCCTCGAAGTTATTTCCTCTGAGGTATCATTTTGTGTAGCTTTGTTCTTTCTTTTGGTTGGTTTGAAACTTGAATGTCCAATGATTTCCTCTAATTACTTGTTATTGCGACAGCGAGTTTTGGATCACGTTGTAATTCCTGTTTTCATGAGTATAATCTTGTAGAAATTTCAGTTGCCCAAATTGATCTTATTTGGTGTTGGTAGGTCTCATCTTCTGTCAAATATAAGGTGGAGCCAGTAAGTGTGGGTGGACTCACTATGTTTAAGGTAAAGATTCCTGTTATTTCAAAAAGGGTTCTGAAAGTAGATTAAGAAACAAAACATATGTTAGTAGATAAGCATAATTTGTAAAAATAGTGAACCAAAACAAAATCACTTGACAGTGGACTTAGCTCTGAGTTTTGGCACAAAATTGAGATCTTACATGTAATAATGTGGCGTGTTTGCTAAAATGTAAAGTCAGGAGAGTTGTTTTCTTCTTTTGTTTACTAGCTTTGCCATCTTATGATTTCTGTTCGACATACAGTGATGTACTTAAAGCTATTTACCTGTCTATAGGGACGGGTCAGCACTCAGGAGGTTTTCTCCTTGTCCAACTCTGATTTGGTTCCTGGTGAATATGAAGGTAATCGAGTATCCAAGTTTCCTGGTTCGTGCTAGAAAAGCACGTATCTGGATTATGGCAATTGGCAACAAACATGATTATGATGCATGTACTTTGTGGGCTGTAGGGGGGCTTAAGTTATGGGAAGGCGCATTAGATCTTGTCAAGGCTCTTTGTGGGGAAGTTGAAAATGGGCATTTATCATTTGCTGGAAAACGAGTTCTAGAGGTGGGCATCTTGATATTCAGGTGATTCTATACTGTCTGGAGATTGTAGACGAATACATCTGCATGCTAGATAATGTTTTTAGAGCATTATGATCATTCATGTGTGGATAGGATGGTTGTAAACTTCTTTCAGATCTATTATTTAAACCTCAAACTTGTCTTAAAAAATGACCATTACTTATTGTTCTTTTGCTCCTATTGATGGCGTTTACTTGTGACTATGATTGGATTAGATTGCAAACTTTATTTAGCATTTCTGATTAGAAATATGGTTGATTTGGAGCCAGAAATTTATAATCAATTTCACGAGTGGGATCATCGCTAAAGACGGGTTTATCTTGATGATAAGCAACTGTTAACAGATTTTAGTATCTTCATGCATGTAACAGTTGGAATTGTGAATAGAAGAAAATTAATATATATATAACCACATTTGATTTACTTAACGTACTGCAAAACTTGCTAGTAATTGAGCTTTAGTTTGATCATTAAGAAACATTTCATGGTCAAGAGAATTATCCTCTTTATCATTTTTAACAGTTTAGAAATTCATGTTTGACTCCCAGTGCAGTGTGTATGTAGTTGGTCAAATGTGAATAAACAATCATCTGCTGATTAAATAGTTTTACATTAGAACAGTGTTCCAGGTCAATGTTATAATTTTCTTAGATGTTTCATTTTTTCTGTTTTTTTTTAATTTTTATTTTGAGATTTTAATCAGGTTCTCACATTCAAAGAACTGGGTTTATTTTATGTGCGTTTAATTTAAAAGTTAGTATTCTATATTTATCATGTATATTTTCCTAATGCCCATGTATAAGATCATTTCCTTTTCCTGATGTTTGTTGGGAAGGATCTTTCGATTCCCATACTGGGTTGCGGTTGGTCCAATTTCCTGGATACTGCAAAAGGAAGTTTCTTTCTGACATCTATAAAATCCATCTCTTCCTCATTGACTTTGCGCTCAATGCCTTTTTCAGATCGGATGTGGTCATGGTCTTCCTGGTATCTATGCATGCCTTGAGGTAAATATATTCTTTTGTTTTGTTTCATTACACTTACAGATTAAATGCATGAAAATCTCTATGGTTGTTTACGCCCCTCGCCATTACCCTCTCTCTCTTAGGATGCAGCTGCTGTACATTTCCAGGATTTCAATGCTGAAGTCCTCAGGTGTCTTACCATACCAAATGTGACTGCAAATCTTTCAAAAATGGCTGCAGAATCAGAGGCAAGATCAGAAGTTCGTTACTTTGCTGGTGATTGGAGTGAAATGCATAAAATTCTCCCTTTTGCACCTGACAATGTGGTGAAGCTAAGTGGAAGTTGTGATCCCGGACAAGTTTCTGGCTATGACATTGTTTTAATGGCAGAGACAGTTTATTCTCTTTCCACACTTAAAACTCTGTATAAGCTTATCACACAGGTAAGAACTTATACAGATGTCCATCTAATGTTGTATTATTATTATAGCTTCATTAATTCTAATGTAGTTTTAAACATATTAATAGTGCTTGAGCCGTCCTCATGGAGTTGTATACTTGGCTGGAAAGAAGCATTACTTTGGAGTTGGAGGAGGAACTCGACGCTTTCTATCTGTGTTAGAAAAAGAAGGTGAGCTGGTCTCCCAAAAATTCCTGTGAAAATTATGCTTAAACACTATACTTCTCCCTGCTCCGATGAAGAAGGAGCTATATTAAAAGAAATACGATATGCTTGCTGAGTATCCATTGGAGAACATTTCTTATGAAGCAGTTATAGACAACATTTCTAGACTAAAATATGATGGTTTCGTGAATGTTGTATGGGGCGCTGGCTGATCCATGACAAATGTTTATTTGAAATTTCTGGGACATGATCTTGATCATTTTGACATTTTCTGTGAGTTCATATTTTGAGAACTAAATTAACTTTCAAATCTTTAGGCAACTCGCACTCTTCAAAATCCAGTATATTTGGTGTTGAAAGATTGATCCGCTTCAAGAAACCACTGACAGTTATACTCCTATTCATTTATGTCATCAAAGGAGATCTGTTTATTTATTTACTTTCTGATAATTGGGGTGTTGGGACTCATTTTTGCATTGTACTCATGCCTCACCCTACTAATCCCTATAGGACTTCGCCGACCTTTAGTAATCCGTAGTAAGTTTTCGCCCACTCCAAAACCAGCTTCGGGAGACAACAAGGGATTTAGATCATTTAGGAAGAATTAATACTTGTGATCTTTTAGATTTTTTCCAGAATTAGTTCGCCAAGAAAAAGTTTACATGCACTACTGATGGTTATGATTTCACAGACGGATTTATGATTCGATCACTTTTTTTAGGAGGGATCTATTTCTTGAATTGATTGTTTATCGTCTGCCGTTACAGGTGAAATGGTTTCCAAACTGATTGCAGAAGTTGCCGATGGTTCATCCAACGTTCGAGAAGTGTGGAAGCTCTCACTGAAATAGGTTAGAAATCCTAGCTTTTTTGTACTAAATTACGTTCCTCTAGAAATGATTTGAGAAATTCCTTAAAATAAAATGCTAATGTGATTCATGTTCATTTAAGTTAGTGATACATTCATAAGAGTAATTCAATGGTAATCCTTGCAGAAAGAATTACCGGGGCGACTTATGCTTCACATCGACAGATAAGGCTTGTGTTCATTTAATCACAATCCACCCCCAACAATTTCTTTTTCTATATTTAGACAAGATTTGGAATAGGATTCTAAGTTTTATTGTATTTCGTTAAAATAACTTAAAAATATGTTGGGTTTGAAAGTAGTGATACAGTTTTGCTTAGCTAGAAGCAACCACCTGGTGCTAAAATTCATTACTCACAATAATGCTAAATCGTGACTCGTAGCCACGATTGACCTTAAAATATGTGAAGGGGTGGGAAGAATGCTATTATAATTGTGCTTGGGAGATGCGTGGTCTCATGCAAGGATGGGAGTGGAATCACAAAGCTGAAGACATTGCTGAAAAAGAAATTCAAAATTATGGATTTTCGAATCCGGAGTGAAGGCTAAAAAAGTTCCTATATCTCTCGTCGGAGTTTATATCTTGGACAAATCGATAAAATCCTTGGATATGAATGCTACTTTGGGAAGCACATTCCGAATCATCCATGAAAAATTCTCTATCGACGAAGGAAGAAGTTGAATAGACGATAAAAGTGGCCCGTGTTTGCCTTGTTGAGAATGCCATGCCAAATGTAGTGAGTTAAATGGATTTTAAATATAGTGTCTAGTGTTTGTCTATTTTGATCATTTATTTTCAAAACATTCATATGATCCTAGAATTTTCAGTTCATATATATATATATGTGTGTATGTTTTGTCTTTCTACTCGTAAAATATATATTTTTGATTACTTTTTAAAAGTACATTTCATGGGTGTGAAATGTCATTTTAGGATCAGTTGAAATAATG

mRNA sequence

ATCCAACATAATCTTCCACTGCAGTTACTCCATCCGACACATATATTTCGTGGATTTCCCTACCTTCACACAACGTCAACAGCCCGTCAAGAGAATTTTCACTACTTTTTCTGCTTCAATGCCAAAGTACGATGTCAATGTGAAAGAAAAAAAAAAAAAACCTTGGACTCAGAAGGTTTGGAGGCGGAGCTCGAACGCGAAAGCTCTGTTCCGCCGTTGAGAAGGAGGAACTCTCAGGGGAGCAACCAGCGATGGCGGCGAACTCTTCGAGTCCGGCTTTCGAAAATTTCCGGTTGTTTGCGTCAGTGGAAGACGAGAAGTCCAATCCAGGTCTCGGTATAGGTTTTCTCGACTCTCAACAGCCTTCTCTTCCACCTCCACCTCCTTGCCTCGAAGTTATTTCCTCTGAGGTCTCATCTTCTGTCAAATATAAGGTGGAGCCAGTAAGTGTGGGTGGACTCACTATGTTTAAGGGACGGGTCAGCACTCAGGAGGTTTTCTCCTTGTCCAACTCTGATTTGGTTCCTGGTGAATATGAAGGGGGGCTTAAGTTATGGGAAGGCGCATTAGATCTTGTCAAGGCTCTTTGTGGGGAAGTTGAAAATGGGCATTTATCATTTGCTGGAAAACGAGTTCTAGAGATCGGATGTGGTCATGGTCTTCCTGGTATCTATGCATGCCTTGAGGATGCAGCTGCTGTACATTTCCAGGATTTCAATGCTGAAGTCCTCAGGTGTCTTACCATACCAAATGTGACTGCAAATCTTTCAAAAATGGCTGCAGAATCAGAGGCAAGATCAGAAGTTCGTTACTTTGCTGGTGATTGGAGTGAAATGCATAAAATTCTCCCTTTTGCACCTGACAATGTGGTGAAGCTAAGTGGAAGTTGTGATCCCGGACAAGTTTCTGGCTATGACATTGTTTTAATGGCAGAGACAGTTTATTCTCTTTCCACACTTAAAACTCTGTATAAGCTTATCACACAGTGCTTGAGCCGTCCTCATGGAGTTGTATACTTGGCTGGAAAGAAGCATTACTTTGGAGTTGGAGGAGGAACTCGACGCTTTCTATCTGTGTTAGAAAAAGAAGGTGAAATGGTTTCCAAACTGATTGCAGAAGTTGCCGATGGTTCATCCAACGTTCGAGAAGTGTGGAAGCTCTCACTGAAATAGAAAGAATTACCGGGGCGACTTATGCTTCACATCGACAGATAAGGCTTGTGTTCATTTAATCACAATCCACCCCCAACAATTTCTTTTTCTATATTTAGACAAGATTTGGAATAGGATTCTAAGTTTTATTGTATTTCGTTAAAATAACTTAAAAATATGTTGGGTTTGAAAGTAGTGATACAGTTTTGCTTAGCTAGAAGCAACCACCTGGTGCTAAAATTCATTACTCACAATAATGCTAAATCGTGACTCGTAGCCACGATTGACCTTAAAATATGTGAAGGGGTGGGAAGAATGCTATTATAATTGTGCTTGGGAGATGCGTGGTCTCATGCAAGGATGGGAGTGGAATCACAAAGCTGAAGACATTGCTGAAAAAGAAATTCAAAATTATGGATTTTCGAATCCGGAGTGAAGGCTAAAAAAGTTCCTATATCTCTCGTCGGAGTTTATATCTTGGACAAATCGATAAAATCCTTGGATATGAATGCTACTTTGGGAAGCACATTCCGAATCATCCATGAAAAATTCTCTATCGACGAAGGAAGAAGTTGAATAGACGATAAAAGTGGCCCGTGTTTGCCTTGTTGAGAATGCCATGCCAAATGTAGTGAGTTAAATGGATTTTAAATATAGTGTCTAGTGTTTGTCTATTTTGATCATTTATTTTCAAAACATTCATATGATCCTAGAATTTTCAGTTCATATATATATATATGTGTGTATGTTTTGTCTTTCTACTCGTAAAATATATATTTTTGATTACTTTTTAAAAGTACATTTCATGGGTGTGAAATGTCATTTTAGGATCAGTTGAAATAATG

Coding sequence (CDS)

ATGGCGGCGAACTCTTCGAGTCCGGCTTTCGAAAATTTCCGGTTGTTTGCGTCAGTGGAAGACGAGAAGTCCAATCCAGGTCTCGGTATAGGTTTTCTCGACTCTCAACAGCCTTCTCTTCCACCTCCACCTCCTTGCCTCGAAGTTATTTCCTCTGAGGTCTCATCTTCTGTCAAATATAAGGTGGAGCCAGTAAGTGTGGGTGGACTCACTATGTTTAAGGGACGGGTCAGCACTCAGGAGGTTTTCTCCTTGTCCAACTCTGATTTGGTTCCTGGTGAATATGAAGGGGGGCTTAAGTTATGGGAAGGCGCATTAGATCTTGTCAAGGCTCTTTGTGGGGAAGTTGAAAATGGGCATTTATCATTTGCTGGAAAACGAGTTCTAGAGATCGGATGTGGTCATGGTCTTCCTGGTATCTATGCATGCCTTGAGGATGCAGCTGCTGTACATTTCCAGGATTTCAATGCTGAAGTCCTCAGGTGTCTTACCATACCAAATGTGACTGCAAATCTTTCAAAAATGGCTGCAGAATCAGAGGCAAGATCAGAAGTTCGTTACTTTGCTGGTGATTGGAGTGAAATGCATAAAATTCTCCCTTTTGCACCTGACAATGTGGTGAAGCTAAGTGGAAGTTGTGATCCCGGACAAGTTTCTGGCTATGACATTGTTTTAATGGCAGAGACAGTTTATTCTCTTTCCACACTTAAAACTCTGTATAAGCTTATCACACAGTGCTTGAGCCGTCCTCATGGAGTTGTATACTTGGCTGGAAAGAAGCATTACTTTGGAGTTGGAGGAGGAACTCGACGCTTTCTATCTGTGTTAGAAAAAGAAGGTGAAATGGTTTCCAAACTGATTGCAGAAGTTGCCGATGGTTCATCCAACGTTCGAGAAGTGTGGAAGCTCTCACTGAAATAG

Protein sequence

MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKYKVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGHLSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAAESEARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLSTLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGSSNVREVWKLSLK
Homology
BLAST of MC05g0726 vs. ExPASy Swiss-Prot
Match: Q55DL2 (Histidine protein methyltransferase 1 homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0270580 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.1e-37
Identity = 88/222 (39.64%), Postives = 130/222 (58.56%), Query Frame = 0

Query: 85  LSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGHLSFAGKRVLEIGCGHGLPGIYACL 144
           L  +DL+PG YEGG KLWE ++D++  L  E     +  +GK+VLEIGCGHGLPGIY CL
Sbjct: 116 LDKTDLIPGVYEGGFKLWECSIDIINYLFEE----KIDLSGKKVLEIGCGHGLPGIY-CL 175

Query: 145 EDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAAESEARSEVRYFAGDWSEMHKILPFAPD 204
            + + V FQD+N EV+  LT PNV  N   +       +  +Y +GDW  + ++L     
Sbjct: 176 LNGSIVTFQDYNEEVIYNLTQPNVLINGGDI-------NRAKYISGDWKFVDQLL----- 235

Query: 205 NVVKLSGSCDPGQVSGYDIVLMAETVYSLSTLKTLYKLITQCLSRPHGVVYLAGKKHYFG 264
                       +   +DI+L ++T+Y++ + K LY LI+  L   +G  YLA K +YFG
Sbjct: 236 ------------KNEKFDIILTSDTLYNVGSFKKLYNLISNHL-ESNGKCYLASKTYYFG 295

Query: 265 VGGGTRRFLSVLEKEGEMVSKLIAEVADGSSNVREVWKLSLK 307
           VGGG R+F  +L+    +  K + ++ DG SNVREV +++ K
Sbjct: 296 VGGGIRKFEELLKILNRLSIKTVRDIKDGLSNVREVVEITNK 307

BLAST of MC05g0726 vs. ExPASy Swiss-Prot
Match: Q4KM84 (Histidine protein methyltransferase 1 homolog OS=Rattus norvegicus OX=10116 GN=Mettl18 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 9.3e-34
Identity = 105/308 (34.09%), Postives = 159/308 (51.62%), Query Frame = 0

Query: 17  ASVEDEKSNPGL----GIGFLDSQQPSLPP------PPPCLEVISSEVSSSV--KYKVEP 76
           AS ED  S P      G+     +QPSL P      P  C +V+ ++V   +     V  
Sbjct: 60  ASSEDTDSPPSTADRSGVPEACEKQPSLKPAKEHVIPKDCDQVLENKVLEMLPGSQHVST 119

Query: 77  VSVGGLTM---FKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGHL 136
             V  +++   F G     + FS S+SDL+PG YEGGLK+WE   DL+           +
Sbjct: 120 AVVKTISLKEKFPGENIVSQSFS-SHSDLIPGVYEGGLKIWECTFDLMTYF----TKAKV 179

Query: 137 SFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTAN---------L 196
            FAG++VL++GCG GL GI A    A  VHFQD+N  V+  +T+PNV AN         +
Sbjct: 180 KFAGQKVLDLGCGSGLLGITASKGGAREVHFQDYNGLVIDEVTLPNVVANVPLQGDSNGI 239

Query: 197 SKMAAESEARSEV-------RYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVL 256
           ++ A + + +SEV       R F+G+W+E  K++  +    VK            YD++L
Sbjct: 240 NEPAGKRQRKSEVAQETCKCRLFSGEWAEFCKLV-LSEKLFVK------------YDLIL 299

Query: 257 MAETVYSLSTLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSK 294
            +ET+Y+     TL++ + + LSR +G V LA K HYFGVGGG   F   +E++G   ++
Sbjct: 300 TSETIYNPDYYSTLHETLLRLLSR-NGRVLLASKAHYFGVGGGVHLFQKFVEEKGVFETR 348

BLAST of MC05g0726 vs. ExPASy Swiss-Prot
Match: Q9CZ09 (Histidine protein methyltransferase 1 homolog OS=Mus musculus OX=10090 GN=Mettl18 PE=2 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 3.3e-31
Identity = 87/237 (36.71%), Postives = 125/237 (52.74%), Query Frame = 0

Query: 73  FKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGHLSFAGKRVLEIG 132
           F G     + FS S+SDL+PG YEGGLK+WE   DL+           + FAG++VL++G
Sbjct: 131 FPGENIVSKSFS-SHSDLIPGVYEGGLKIWECTFDLLTYF----TKAKVKFAGQKVLDLG 190

Query: 133 CGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANL-------------SKMAAES 192
           CG GL GI A    A  VHFQD+N  V+  +T+PNV AN+              K   +S
Sbjct: 191 CGSGLLGITASKGGAREVHFQDYNGLVIDEVTLPNVVANVPLQDDSNGKNEPDGKRQRKS 250

Query: 193 EARSEV---RYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLSTL 252
           E   E+   R F+G+W+E  K++       VK            YD++L +ET+Y+    
Sbjct: 251 EVGREICKCRLFSGEWAEFCKLV-LREKLFVK------------YDLILTSETIYNPDYY 310

Query: 253 KTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 294
            TL++   + LSR  G V LA K HYFGVGGG   F   +E++G   ++ +  + +G
Sbjct: 311 STLHETFLRLLSR-SGRVLLASKAHYFGVGGGVHLFQKFVEEKGVFETRTLEVIDEG 348

BLAST of MC05g0726 vs. ExPASy Swiss-Prot
Match: O95568 (Histidine protein methyltransferase 1 homolog OS=Homo sapiens OX=9606 GN=METTL18 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.7e-30
Identity = 80/236 (33.90%), Postives = 124/236 (52.54%), Query Frame = 0

Query: 73  FKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGHLSFAGKRVLEIG 132
           F G     + FS S+SDL+ G YEGGLK+WE   DL+           + FAGK+VL++G
Sbjct: 141 FPGENIVSKSFS-SHSDLITGVYEGGLKIWECTFDLL----AYFTKAKVKFAGKKVLDLG 200

Query: 133 CGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAAESEARS--------- 192
           CG GL GI A    +  +HFQD+N+ V+  +T+PNV AN +    E++            
Sbjct: 201 CGSGLLGITAFKGGSKEIHFQDYNSMVIDEVTLPNVVANSTLEDEENDVNEPDVKRCRKP 260

Query: 193 ------EVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLSTLK 252
                 + R+F+G+WSE  K++  +    VK            YD++L +ET+Y+     
Sbjct: 261 KVTQLYKCRFFSGEWSEFCKLVLSSEKLFVK------------YDLILTSETIYNPDYYS 320

Query: 253 TLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 294
            L++   + LS+ +G V LA K HYFGVGGG   F   +E+     ++++  + +G
Sbjct: 321 NLHQTFLRLLSK-NGRVLLASKAHYFGVGGGVHLFQKFVEERDVFKTRILKIIDEG 358

BLAST of MC05g0726 vs. ExPASy Swiss-Prot
Match: Q2KIJ2 (Histidine protein methyltransferase 1 homolog OS=Bos taurus OX=9913 GN=METTL18 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.2e-30
Identity = 84/248 (33.87%), Postives = 129/248 (52.02%), Query Frame = 0

Query: 65  VSVGGLTMFKGRVSTQEVFS---LSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGHL 124
           +S+   T+ K     + + S    S+SDL+ G YEGGLK+WE   DL+  L        +
Sbjct: 129 ISIMKTTLLKENFPGENIISKSFSSHSDLISGVYEGGLKIWECTFDLLAYL----TKAKV 188

Query: 125 SFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAAESEA 184
            FAGK+VL++GCG GL GI A    A  +HFQD+N+ V+  +T+PNV AN +    E++ 
Sbjct: 189 KFAGKKVLDLGCGSGLLGIMALKGGAKEIHFQDYNSVVIDEVTLPNVVANSTLEDEENDV 248

Query: 185 ---------RSEV-------RYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVL 244
                    RS V       R+F+G+WSE  K++  +     K            YD++L
Sbjct: 249 NEPDVKRLRRSTVAQELCKCRFFSGEWSEFCKLVLSSEKLFEK------------YDLIL 308

Query: 245 MAETVYSLSTLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSK 294
            +ET+Y+      L++   + L + +G V LA K HYFGVGGGT  F   +E+     ++
Sbjct: 309 TSETIYNPDYYVPLHQTFLRLLDK-NGQVLLASKVHYFGVGGGTHLFQKFVEERNVFETR 359

BLAST of MC05g0726 vs. NCBI nr
Match: XP_022138235.1 (histidine protein methyltransferase 1 homolog [Momordica charantia] >XP_022138236.1 histidine protein methyltransferase 1 homolog [Momordica charantia])

HSP 1 Score: 612 bits (1577), Expect = 5.49e-220
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 0

Query: 1   MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 60
           MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY
Sbjct: 49  MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 108

Query: 61  KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 120
           KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH
Sbjct: 109 KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 168

Query: 121 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAAESE 180
           LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAAESE
Sbjct: 169 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAAESE 228

Query: 181 ARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLSTLKTLY 240
           ARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLSTLKTLY
Sbjct: 229 ARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLSTLKTLY 288

Query: 241 KLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGSSNVREV 300
           KLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGSSNVREV
Sbjct: 289 KLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGSSNVREV 348

Query: 301 WKLSLK 306
           WKLSLK
Sbjct: 349 WKLSLK 354

BLAST of MC05g0726 vs. NCBI nr
Match: XP_022979862.1 (histidine protein methyltransferase 1 homolog [Cucurbita maxima] >XP_022979863.1 histidine protein methyltransferase 1 homolog [Cucurbita maxima] >XP_022979864.1 histidine protein methyltransferase 1 homolog [Cucurbita maxima])

HSP 1 Score: 530 bits (1366), Expect = 1.38e-188
Identity = 270/312 (86.54%), Postives = 283/312 (90.71%), Query Frame = 0

Query: 1   MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 60
           MAANSSSPAFENFRLF S EDEK NPGL +GFLDSQQP LPPPPPCLEV+SSEV SSVKY
Sbjct: 1   MAANSSSPAFENFRLFGSAEDEKPNPGLSLGFLDSQQPPLPPPPPCLEVLSSEVCSSVKY 60

Query: 61  KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 120
            VEPV+VG +TMFKGRVSTQEVFSLSNSDLVPG+YEGGLKLWEGALDLVKALCGEVENGH
Sbjct: 61  NVEPVTVGEITMFKGRVSTQEVFSLSNSDLVPGKYEGGLKLWEGALDLVKALCGEVENGH 120

Query: 121 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAA--- 180
           LSFAGKRVLE+GCGHGLPGIYACLEDAAAVHFQDFNAEVLR LTIPNVTANL+K AA   
Sbjct: 121 LSFAGKRVLELGCGHGLPGIYACLEDAAAVHFQDFNAEVLRYLTIPNVTANLTKKAALPT 180

Query: 181 ---ESEARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLS 240
              ES+A S  RYFAGDWSEMHKILPFA D+  KLS +      +GYDIVLMAETVYSLS
Sbjct: 181 NPTESDAGSAARYFAGDWSEMHKILPFASDDKEKLSEN-----FNGYDIVLMAETVYSLS 240

Query: 241 TLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGS 300
           TLKTLYKLITQCLSRPHGVVY+AGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGS
Sbjct: 241 TLKTLYKLITQCLSRPHGVVYMAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGS 300

Query: 301 SNVREVWKLSLK 306
           SNVREVWK+SLK
Sbjct: 301 SNVREVWKISLK 307

BLAST of MC05g0726 vs. NCBI nr
Match: XP_023528866.1 (histidine protein methyltransferase 1 homolog [Cucurbita pepo subsp. pepo] >XP_023528867.1 histidine protein methyltransferase 1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 530 bits (1365), Expect = 2.03e-188
Identity = 268/313 (85.62%), Postives = 283/313 (90.42%), Query Frame = 0

Query: 1   MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 60
           MAANSSSPAFENFRLF S ED+K NPGL +GFLDSQQP LPPPPPCLEV+SSEV SSVK+
Sbjct: 1   MAANSSSPAFENFRLFGSAEDDKPNPGLSLGFLDSQQPPLPPPPPCLEVLSSEVCSSVKF 60

Query: 61  KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 120
            VEPV+VG +TMFKGRVSTQEVFSLSNSDLVPG+YEGGLKLWEGALDLVKALCGEVENGH
Sbjct: 61  NVEPVTVGEITMFKGRVSTQEVFSLSNSDLVPGKYEGGLKLWEGALDLVKALCGEVENGH 120

Query: 121 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMA---- 180
           LSFAGKRVLE+GCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANL+K A    
Sbjct: 121 LSFAGKRVLELGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLTKKAEALP 180

Query: 181 ---AESEARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSL 240
               ES+A S  RYFAGDWSEMHKILPFA D+  KLS +      +GYDIVLMAETVYSL
Sbjct: 181 TNPTESDAGSAARYFAGDWSEMHKILPFASDDKEKLSEN-----FNGYDIVLMAETVYSL 240

Query: 241 STLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 300
           STLKTLYKLITQCLSRPHGVVY+AGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG
Sbjct: 241 STLKTLYKLITQCLSRPHGVVYMAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 300

Query: 301 SSNVREVWKLSLK 306
           SSNVREVWK+SLK
Sbjct: 301 SSNVREVWKISLK 308

BLAST of MC05g0726 vs. NCBI nr
Match: XP_038888789.1 (histidine protein methyltransferase 1 homolog [Benincasa hispida] >XP_038888856.1 histidine protein methyltransferase 1 homolog [Benincasa hispida])

HSP 1 Score: 529 bits (1363), Expect = 4.09e-188
Identity = 271/313 (86.58%), Postives = 281/313 (89.78%), Query Frame = 0

Query: 1   MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 60
           MAANSSSPAFENFRLF S EDEK N GL +GFLDSQQP LPPPPP LEV+SSEVSSSVKY
Sbjct: 1   MAANSSSPAFENFRLFGSAEDEKHNLGLSLGFLDSQQPPLPPPPPTLEVLSSEVSSSVKY 60

Query: 61  KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 120
            VEPVSVGGLTM KGRVSTQEVFSLSNSDLVPG+YEGGLKLWEGALDL+KALCGEVE+GH
Sbjct: 61  NVEPVSVGGLTMLKGRVSTQEVFSLSNSDLVPGKYEGGLKLWEGALDLIKALCGEVEDGH 120

Query: 121 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKM----- 180
           LSF+GKRVLE+GCGHGLPGIYACL+DAAAVHFQDFNAEVLRCLTIPNV ANLSK      
Sbjct: 121 LSFSGKRVLELGCGHGLPGIYACLKDAAAVHFQDFNAEVLRCLTIPNVAANLSKKDETLS 180

Query: 181 --AAESEARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSL 240
               ES+A S  RYFAGDWSEMHKILPFA DN  +LSGS D     GYDIVLMAETVYSL
Sbjct: 181 INPTESDAGSAFRYFAGDWSEMHKILPFASDNEEELSGSFD-----GYDIVLMAETVYSL 240

Query: 241 STLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 300
           STLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG
Sbjct: 241 STLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 300

Query: 301 SSNVREVWKLSLK 306
           SSNVREVWKLSLK
Sbjct: 301 SSNVREVWKLSLK 308

BLAST of MC05g0726 vs. NCBI nr
Match: KAG6582128.1 (Histidine protein methyltransferase 1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 527 bits (1358), Expect = 2.36e-187
Identity = 267/313 (85.30%), Postives = 281/313 (89.78%), Query Frame = 0

Query: 1   MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 60
           MAANSSSPAFENFRLF S ED+K NPG  +GFLDSQQP LPPPPPCLEV+SSEV SSVKY
Sbjct: 1   MAANSSSPAFENFRLFGSAEDDKPNPGFSLGFLDSQQPPLPPPPPCLEVLSSEVCSSVKY 60

Query: 61  KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 120
            VEPV+VG +TMFKGRVSTQEVFSLSNSDLVPG+YEGGLKLWEGALDLVKALCGEVENGH
Sbjct: 61  NVEPVTVGEITMFKGRVSTQEVFSLSNSDLVPGKYEGGLKLWEGALDLVKALCGEVENGH 120

Query: 121 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMA---- 180
           LSFAGKRVLE+GCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANL+K A    
Sbjct: 121 LSFAGKRVLELGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLTKKAEALP 180

Query: 181 ---AESEARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSL 240
               ES+A S   YFAGDWSEMHKILPFA D+  KLS +      +GYDIVLMAETVYSL
Sbjct: 181 TNPTESDAESVACYFAGDWSEMHKILPFASDDKEKLSEN-----FNGYDIVLMAETVYSL 240

Query: 241 STLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 300
           STLKTLYKLITQCLSRPHGVVY+AGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG
Sbjct: 241 STLKTLYKLITQCLSRPHGVVYMAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 300

Query: 301 SSNVREVWKLSLK 306
           SSNVREVWK+SLK
Sbjct: 301 SSNVREVWKISLK 308

BLAST of MC05g0726 vs. ExPASy TrEMBL
Match: A0A6J1C961 (histidine protein methyltransferase 1 homolog OS=Momordica charantia OX=3673 GN=LOC111009460 PE=4 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 2.66e-220
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 0

Query: 1   MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 60
           MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY
Sbjct: 49  MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 108

Query: 61  KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 120
           KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH
Sbjct: 109 KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 168

Query: 121 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAAESE 180
           LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAAESE
Sbjct: 169 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAAESE 228

Query: 181 ARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLSTLKTLY 240
           ARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLSTLKTLY
Sbjct: 229 ARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLSTLKTLY 288

Query: 241 KLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGSSNVREV 300
           KLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGSSNVREV
Sbjct: 289 KLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGSSNVREV 348

Query: 301 WKLSLK 306
           WKLSLK
Sbjct: 349 WKLSLK 354

BLAST of MC05g0726 vs. ExPASy TrEMBL
Match: A0A6J1IXI7 (histidine protein methyltransferase 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111479428 PE=4 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 6.66e-189
Identity = 270/312 (86.54%), Postives = 283/312 (90.71%), Query Frame = 0

Query: 1   MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 60
           MAANSSSPAFENFRLF S EDEK NPGL +GFLDSQQP LPPPPPCLEV+SSEV SSVKY
Sbjct: 1   MAANSSSPAFENFRLFGSAEDEKPNPGLSLGFLDSQQPPLPPPPPCLEVLSSEVCSSVKY 60

Query: 61  KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 120
            VEPV+VG +TMFKGRVSTQEVFSLSNSDLVPG+YEGGLKLWEGALDLVKALCGEVENGH
Sbjct: 61  NVEPVTVGEITMFKGRVSTQEVFSLSNSDLVPGKYEGGLKLWEGALDLVKALCGEVENGH 120

Query: 121 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMAA--- 180
           LSFAGKRVLE+GCGHGLPGIYACLEDAAAVHFQDFNAEVLR LTIPNVTANL+K AA   
Sbjct: 121 LSFAGKRVLELGCGHGLPGIYACLEDAAAVHFQDFNAEVLRYLTIPNVTANLTKKAALPT 180

Query: 181 ---ESEARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLS 240
              ES+A S  RYFAGDWSEMHKILPFA D+  KLS +      +GYDIVLMAETVYSLS
Sbjct: 181 NPTESDAGSAARYFAGDWSEMHKILPFASDDKEKLSEN-----FNGYDIVLMAETVYSLS 240

Query: 241 TLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGS 300
           TLKTLYKLITQCLSRPHGVVY+AGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGS
Sbjct: 241 TLKTLYKLITQCLSRPHGVVYMAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGS 300

Query: 301 SNVREVWKLSLK 306
           SNVREVWK+SLK
Sbjct: 301 SNVREVWKISLK 307

BLAST of MC05g0726 vs. ExPASy TrEMBL
Match: A0A6J1GX54 (histidine protein methyltransferase 1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111457962 PE=4 SV=1)

HSP 1 Score: 526 bits (1355), Expect = 3.28e-187
Identity = 266/313 (84.98%), Postives = 281/313 (89.78%), Query Frame = 0

Query: 1   MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 60
           MAANSSSPAFENFRLF S ED+K NPG  +GFLDSQQP LPPPPPCLEV+SSEV SSVKY
Sbjct: 1   MAANSSSPAFENFRLFGSAEDDKPNPGFSLGFLDSQQPPLPPPPPCLEVLSSEVCSSVKY 60

Query: 61  KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 120
            VEPV+VG +TMFKGRVSTQEVFSLSNSDLVPG+YEGGLKLWEGALDLVKALCGEVENGH
Sbjct: 61  NVEPVTVGEITMFKGRVSTQEVFSLSNSDLVPGKYEGGLKLWEGALDLVKALCGEVENGH 120

Query: 121 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKMA---- 180
           LSFAGKRVLE+GCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANL+K A    
Sbjct: 121 LSFAGKRVLELGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLTKKAEALP 180

Query: 181 ---AESEARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSL 240
               ES+A S   YFAGDWSEMHKILPFA D+  KLS +      +GYDIVLMAETVYSL
Sbjct: 181 TNPTESDAESVACYFAGDWSEMHKILPFASDDKEKLSEN-----FNGYDIVLMAETVYSL 240

Query: 241 STLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 300
           STLKTLY+LITQCLSRPHGVVY+AGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG
Sbjct: 241 STLKTLYRLITQCLSRPHGVVYMAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 300

Query: 301 SSNVREVWKLSLK 306
           SSNVREVWK+SLK
Sbjct: 301 SSNVREVWKISLK 308

BLAST of MC05g0726 vs. ExPASy TrEMBL
Match: A0A0A0LAX3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171830 PE=4 SV=1)

HSP 1 Score: 519 bits (1337), Expect = 1.81e-184
Identity = 265/313 (84.66%), Postives = 277/313 (88.50%), Query Frame = 0

Query: 1   MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 60
           MA NSS+PAF NFRLF S EDEK N G  +GFLDSQQPSLPPPPP LEV+SSEVSSSVKY
Sbjct: 1   MAENSSTPAFGNFRLFGSAEDEKPNLGFSLGFLDSQQPSLPPPPPSLEVLSSEVSSSVKY 60

Query: 61  KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 120
            VEPVSVGGLT+FKGRV TQEVFSLSNSDLVPG YEGGLKLWEGALDLVKALC E+ENGH
Sbjct: 61  NVEPVSVGGLTLFKGRVRTQEVFSLSNSDLVPGTYEGGLKLWEGALDLVKALCEELENGH 120

Query: 121 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKM----- 180
           LSFAGKRVLE+GCGHGLPGIYACL+DAAAVHFQDFNAEVLRCLTIPNV ANLSK      
Sbjct: 121 LSFAGKRVLELGCGHGLPGIYACLKDAAAVHFQDFNAEVLRCLTIPNVAANLSKKYESLP 180

Query: 181 --AAESEARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSL 240
               ES+A S VRYFAGDWSEM+KILPFA +N   LSG+CD     GYDIVLMAETVY+L
Sbjct: 181 SNPTESDAGSAVRYFAGDWSEMYKILPFASNNEENLSGNCD-----GYDIVLMAETVYAL 240

Query: 241 STLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 300
           STLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLI EVADG
Sbjct: 241 STLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIVEVADG 300

Query: 301 SSNVREVWKLSLK 306
           SSNVREVWKLSLK
Sbjct: 301 SSNVREVWKLSLK 308

BLAST of MC05g0726 vs. ExPASy TrEMBL
Match: A0A5D3DJH8 (Histidine protein methyltransferase 1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00030 PE=4 SV=1)

HSP 1 Score: 516 bits (1328), Expect = 4.25e-183
Identity = 264/313 (84.35%), Postives = 276/313 (88.18%), Query Frame = 0

Query: 1   MAANSSSPAFENFRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKY 60
           MA NSS+PAF NFRLF S EDEK N G  +GFLDSQ PSLPPPPP LEV+SSEVSSSVKY
Sbjct: 1   MAENSSTPAFGNFRLFGSAEDEKPNLGFSLGFLDSQHPSLPPPPPSLEVLSSEVSSSVKY 60

Query: 61  KVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGH 120
            VEPVSVG LT+FKGRVSTQEVFSLSNSDLVPG+YEGGLKLWEGALDLVKALC E+ENGH
Sbjct: 61  NVEPVSVGELTLFKGRVSTQEVFSLSNSDLVPGKYEGGLKLWEGALDLVKALCEELENGH 120

Query: 121 LSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLSKM----- 180
           LSFAGKRVLE+GCGHGLPGIYACL+DAAAVHFQDFNAEVLRCLTIPNV ANLSK      
Sbjct: 121 LSFAGKRVLELGCGHGLPGIYACLKDAAAVHFQDFNAEVLRCLTIPNVAANLSKKYESLP 180

Query: 181 --AAESEARSEVRYFAGDWSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSL 240
               ES+A S VRYFAGDWSEM+KILPFA +N   L+GSCD     GYDIVLMAETVYSL
Sbjct: 181 SNPTESDAGSAVRYFAGDWSEMYKILPFASNNEENLNGSCD-----GYDIVLMAETVYSL 240

Query: 241 STLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADG 300
           STLKTLYKLI QCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLI EVADG
Sbjct: 241 STLKTLYKLIIQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIVEVADG 300

Query: 301 SSNVREVWKLSLK 306
           SSNVREVWKLSLK
Sbjct: 301 SSNVREVWKLSLK 308

BLAST of MC05g0726 vs. TAIR 10
Match: AT4G14000.1 (Putative methyltransferase family protein )

HSP 1 Score: 371.3 bits (952), Expect = 7.4e-103
Identity = 180/295 (61.02%), Postives = 235/295 (79.66%), Query Frame = 0

Query: 13  FRLFASVEDEKSNPGLGIGFLDSQQPSLPPPPPCLEVISSEVSSSVKYKVEPVSVGGLTM 72
           F+LF+S        GLG+GFLDS +P+LPPPPP +EV+S EVSSS  ++V+ +++G +T+
Sbjct: 7   FQLFSS----SQGSGLGLGFLDSSEPALPPPPPPVEVLSFEVSSSTDFEVDKLTIGEITL 66

Query: 73  FKGRVSTQEVFSLSNSDLVPGEYEGGLKLWEGALDLVKALCGEVENGHLSFAGKRVLEIG 132
            KGRVST+EVF L NSDLVPG YEGGLKLWEG++DLVKAL  E + G+LSF+GKRVLE+G
Sbjct: 67  LKGRVSTKEVFGLPNSDLVPGVYEGGLKLWEGSIDLVKALEKESQTGNLSFSGKRVLELG 126

Query: 133 CGHGLPGIYACLEDAAAVHFQDFNAEVLRCLTIPNVTANLS-KMAAESEARSEVRYFAGD 192
           CGH LPGIYACL+ + AVHFQDFNAEVLRCLTIPN+ ANLS K ++ S + +EVR+FAG+
Sbjct: 127 CGHALPGIYACLKGSDAVHFQDFNAEVLRCLTIPNLNANLSEKSSSVSVSETEVRFFAGE 186

Query: 193 WSEMHKILPFAPDNVVKLSGSCDPGQVSGYDIVLMAETVYSLSTLKTLYKLITQCLSRPH 252
           WSE+H++LP        ++   +  +  GYDI+LMAET+YS+S  K+ Y+LI +CL+ P 
Sbjct: 187 WSEVHQVLPL-------VNSDGETNKKGGYDIILMAETIYSISAQKSQYELIKRCLAYPD 246

Query: 253 GVVYLAGKKHYFGVGGGTRRFLSVLEKEGEMVSKLIAEVADGSSNVREVWKLSLK 307
           G VY+A KK+YFGVGGGTR+FLS++EK+G + S L+++V DGSSNVREVWKLS K
Sbjct: 247 GAVYMAAKKYYFGVGGGTRQFLSMIEKDGALASTLVSQVTDGSSNVREVWKLSYK 290

BLAST of MC05g0726 vs. TAIR 10
Match: AT2G43320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-52
Identity = 119/331 (35.95%), Postives = 176/331 (53.17%), Query Frame = 0

Query: 37  QPSLPPPPPCLEVISSEVSSSVKYKVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYE 96
           +  L  P P +E+I S+  +  +Y  E +   GL +FKG+VS  ++  LS S+  P + E
Sbjct: 27  EKDLQLPTPAVEIIPSKTVAHHRYSGENLDALGLQVFKGKVSVADIIGLSGSETAPLKNE 86

Query: 97  GGLKLWEGALDLVKALCGEVENGHLSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFN 156
           G LK WE ++ LV  L  E+ +G LSF GKRVLE+GC  G+PGI+ACL+ A++VHFQD +
Sbjct: 87  GSLKSWESSVVLVNVLKNEIRDGQLSFRGKRVLELGCNFGVPGIFACLKGASSVHFQDLS 146

Query: 157 AEVLRCLTIPNVTANLSKMAAESEAR--------------SEVRYFAGDWSEMHKILPFA 216
           AE +RC TIPNV ANL + A + ++R              + VR++AG+W E+  +L   
Sbjct: 147 AETIRCTTIPNVLANL-EQARDRQSRQPESPLTPSRQAISASVRFYAGEWEELSTVLSII 206

Query: 217 PDNVV---------------------------------------KLSGS------CDPGQ 276
             +V+                                       KLSGS       + GQ
Sbjct: 207 RTDVLEPSIPAMNLSFSEEDFMDGCSSQDGSITGQPDFSSRRSRKLSGSRAWERANETGQ 266

Query: 277 VS--GYDIVLMAETVYSLSTLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSV 307
               GYD++LM E  YS+++LK LY LI +CL  P+GV+YLA KK Y G   G +   ++
Sbjct: 267 EGEYGYDVILMTEIPYSITSLKKLYSLIKKCLRPPYGVMYLAAKKQYVGFNSGAKHLRNL 326

BLAST of MC05g0726 vs. TAIR 10
Match: AT2G43320.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-52
Identity = 119/331 (35.95%), Postives = 176/331 (53.17%), Query Frame = 0

Query: 37  QPSLPPPPPCLEVISSEVSSSVKYKVEPVSVGGLTMFKGRVSTQEVFSLSNSDLVPGEYE 96
           +  L  P P +E+I S+  +  +Y  E +   GL +FKG+VS  ++  LS S+  P + E
Sbjct: 27  EKDLQLPTPAVEIIPSKTVAHHRYSGENLDALGLQVFKGKVSVADIIGLSGSETAPLKNE 86

Query: 97  GGLKLWEGALDLVKALCGEVENGHLSFAGKRVLEIGCGHGLPGIYACLEDAAAVHFQDFN 156
           G LK WE ++ LV  L  E+ +G LSF GKRVLE+GC  G+PGI+ACL+ A++VHFQD +
Sbjct: 87  GSLKSWESSVVLVNVLKNEIRDGQLSFRGKRVLELGCNFGVPGIFACLKGASSVHFQDLS 146

Query: 157 AEVLRCLTIPNVTANLSKMAAESEAR--------------SEVRYFAGDWSEMHKILPFA 216
           AE +RC TIPNV ANL + A + ++R              + VR++AG+W E+  +L   
Sbjct: 147 AETIRCTTIPNVLANL-EQARDRQSRQPESPLTPSRQAISASVRFYAGEWEELSTVLSII 206

Query: 217 PDNVV---------------------------------------KLSGS------CDPGQ 276
             +V+                                       KLSGS       + GQ
Sbjct: 207 RTDVLEPSIPAMNLSFSEEDFMDGCSSQDGSITGQPDFSSRRSRKLSGSRAWERANETGQ 266

Query: 277 VS--GYDIVLMAETVYSLSTLKTLYKLITQCLSRPHGVVYLAGKKHYFGVGGGTRRFLSV 307
               GYD++LM E  YS+++LK LY LI +CL  P+GV+YLA KK Y G   G +   ++
Sbjct: 267 EGEYGYDVILMTEIPYSITSLKKLYSLIKKCLRPPYGVMYLAAKKQYVGFNSGAKHLRNL 326

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q55DL23.1e-3739.64Histidine protein methyltransferase 1 homolog OS=Dictyostelium discoideum OX=446... [more]
Q4KM849.3e-3434.09Histidine protein methyltransferase 1 homolog OS=Rattus norvegicus OX=10116 GN=M... [more]
Q9CZ093.3e-3136.71Histidine protein methyltransferase 1 homolog OS=Mus musculus OX=10090 GN=Mettl1... [more]
O955683.7e-3033.90Histidine protein methyltransferase 1 homolog OS=Homo sapiens OX=9606 GN=METTL18... [more]
Q2KIJ26.2e-3033.87Histidine protein methyltransferase 1 homolog OS=Bos taurus OX=9913 GN=METTL18 P... [more]
Match NameE-valueIdentityDescription
XP_022138235.15.49e-220100.00histidine protein methyltransferase 1 homolog [Momordica charantia] >XP_02213823... [more]
XP_022979862.11.38e-18886.54histidine protein methyltransferase 1 homolog [Cucurbita maxima] >XP_022979863.1... [more]
XP_023528866.12.03e-18885.62histidine protein methyltransferase 1 homolog [Cucurbita pepo subsp. pepo] >XP_0... [more]
XP_038888789.14.09e-18886.58histidine protein methyltransferase 1 homolog [Benincasa hispida] >XP_038888856.... [more]
KAG6582128.12.36e-18785.30Histidine protein methyltransferase 1-like protein, partial [Cucurbita argyrospe... [more]
Match NameE-valueIdentityDescription
A0A6J1C9612.66e-220100.00histidine protein methyltransferase 1 homolog OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1IXI76.66e-18986.54histidine protein methyltransferase 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1GX543.28e-18784.98histidine protein methyltransferase 1 homolog OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A0A0LAX31.81e-18484.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171830 PE=4 SV=1[more]
A0A5D3DJH84.25e-18384.35Histidine protein methyltransferase 1-like protein OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
AT4G14000.17.4e-10361.02Putative methyltransferase family protein [more]
AT2G43320.12.0e-5235.95S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT2G43320.22.0e-5235.95S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 76..291
e-value: 7.4E-34
score: 119.0
NoneNo IPR availablePANTHERPTHR14614:SF39HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG-RELATEDcoord: 25..297
IPR019410Lysine methyltransferasePANTHERPTHR14614HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGENcoord: 25..297
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 102..280

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0726.1MC05g0726.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity