MC05g0709 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0709
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description50S ribosomal protein l3
LocationMC05: 5624689 .. 5628654 (-)
RNA-Seq ExpressionMC05g0709
SyntenyMC05g0709
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGCTTTATCCAGAGGTCTCGTTTCTCGTTTCCGTCTTCTCTCCATCAATTCCGGCGCCTCTTCTACAATTTCCCCTTCTTCTTCGCGAACGCATCCCTCTTGTTACTACTTCTTCCGTTCTTTCAGCTCCGAGGATTTGATCCAGGCCGGCGACGTCTCCTCGCCTTCTTCTCCGATCATTGAAGCTAAGCCCGGTGTCATGGCCCCTAACTCGAAGCGGACGGGCGTCATTGCCGTCAAGTGTGGAATGTCGGCGCTGTGGGATAAATGGGGAGCTAGGATACCCATCACCGTGCTCTGGGTTGATGATAATATTGTGTCTCAGGTCAAGACAATCGAGAAAGAAGGTATCACAGCCCTCCAGGTATTACTTAACTCTCTTCTTCTCATTTTCATTCCATTGCCGGAGATAATTTTTGTTTCTCCTTCTTGTTATTATCACTGAGGAGTCTATTTTTGGAACACTATTCACTATTTTAATCTCTTGTGTTATGGCAGCACTTCACTTTCAAGTCCTTGGATTTTGGAATTATACTGAACTGAACTGCACGCCGTTTTGGATTAGTGTCTAACTATGAATACACTTAATTAGACCTAAATATAACCATCTGTGACAGTTTTTTAATTAATAACTGGGTTTTGTGGGTCATTATGCTCATGATCAACCTCCCTAATATTTGGGAGGATTCGCCCACTCCAAAGATAGCTCTGAGAGACAACAAGCGTTTTAGAAATTACTACCATTTACTCCCATTGTTGTTTTCAATATTTCTAAGAAAAAACCAGTTGTCTTGAGTATTAGACCATATGTATGAGGTTTGTTTTTTCAGTTTTAAAAATCTTATATCGCATAGGTAGGTGATAGGACTTGAGTTTATTGACCAAAGGTGCTATGCTATGGTGCTAGTTAGATTGGCTGTGGACAGAAGAAGGCGAAGCATTTGACAAAACCGGAATTAGGACATTTTAGGGCTCAAGGTGTTCCATTGAAGAGGAAACTGAGGGAGTTTCCAGTGTCAGCAGATGCACTTCTTCCAGTTGGTACAACAATTGGTGTTCGTCATTTTGTCCCTGGACAGTATGTTGATGTGACTGGAATCACAAGAGGGAAAGGTTTTCAGGTGAATCCATTGTAGCATTCCTCTAGTTTTTGAACTTGCACCCTTTACCATTTTATTTGTCATTCAATTCCTTATATTTGTTAATATGGTTAAGTTAGTTGCATATTTGAAGCCCTTAAGGTGCTAGGATCATTCGAAATAGAATTGACATGTGAATATCTTTTAGAGATACAATTTATGGATTTTAATGCCATCGCGGGAACATTGAGTGGTATAAATGTATGTCTCTCTTTGAATTTTGGTCAAATTATCTATCCAATGAAATAATGGATTTAACAGATTACGTTGCAACGCAATAATCGGCTGAATAACAAAGAATACTGTAACCACGAGAGTAAGCAATTATTTACACGAAACACAATAACCCCACCCAAAGCTTTTGACATGTTATCATGCTTAATCATGCTCCAACTTGTTTCTTGGCATGCATGCTTAATTAACTAACACTCTGCATGATCTGCAAGGTACAATGTAACTGTGAAAGTTACCATGCTGCACATGTATGTTAGACTAAGAGAACATGAACGGTTTCTCAATGAGGTCATGTGCATCCTTAAGGAGAAAAGAATAACACGATCCATGGATATAAATATAAGCATGAAAGTGTTTGTGGCTACAGAACCCTAGGAAAGACATGTACGTGGCTGATTATTTATGTGGAGCTATGATATTTGCTTTGTCTTCTCTACATGTTTGTGCCAGTTTGGCTGATTTTGGGTTGTATATTTGTGTCCCGAAAGTGATTCACGAGGTCCAATCCACAACTATCATATAAATAACTGTTTTCTTTCCATGTAGGTATATTATTTTTTCTGTTTCCCTAAAAGTGATGTCTCTCATGGCTAATTTTTTTTTTTCTAACTGTGCACAGGGAGTGATGAAAAGGCATGGATTTAAAGGAATGCCAGCATCCCATGGAGCATCATTATCCCATAGAAGCCCTGGTTCTACGGGTCAGAGGGATGCTCCTGGAAAGGTGTGCTTATCTTGACGTTCATTTTTAAGATTGAGTGGTATATCAGTTCTCAAATGCGTTTTAGAGCTACCCTACCTGAAACATGTGCTGGATACTCACATTTGAGATAGTTGCTATTTCGTGTTCGCTTACTGGGTGGGTCTCTCTGATTCTGGGAGTTGTGCTGCTTCTCTCTCTCTCTCTCTCATGTCAATTTGCAAGGCTCCTTCTCAATGAATGAAAATTTTAGAGATGAGAATTAACAATTTACTAAACCATCATGGTGTGGCCTAGTGGTCAATAAGGGTCAAATAACATATGGCTTAGAGGGAATGACTTCAAAAGGTGGCCACATACCTAGAATTTAATATCCTATGAATTACCTTGGCAACCACATGTACTAGGGTCAGACAGTTATCCTGTGAGATTAGTCGAGGTCAGACAGTTATCCTGTGAGATTAGTCGAGGTGCGTGCAAGCTGGCTCGGACACTCACGGATATAAAGAAAAAGAATTTGGAATTTTACTAGGAGTGTTTAGTCAACAAATATGAATAGCTGGCACCCTGGGAAGTGTATGGTTTTTGGATTTAACTATGCTTTTGGATGGAATCAATATTAAGTAATATTTGGAAGTTACTACTTGTGGTTGCAATCTTGAAAATAATATCCTGACGAAACTTAATTTACTAAGTTTTAAGAGATTGAACCTGAATCCCTATACTGAATTTATCTTCAACAGAAGTACTGTGTTTGGATAGAACATACTTTGATTTAGTTTGTTGGAATCATGGAAACTTTTGTGTTGAATGGCAGAATCTCATTCCTGTTTCTAAAGATAAAGGAAAAAAATCCCATAATGTGGAATAATATCCTTGTGACTATGGTCCGTTATGTTAATGTAGTGAAGAATTGTAATTCTTTCTCTCTTTATATCATGTAATTTATGACAGTGAGAAAGCATATACTTATTTTGCAGGTCTTTAAAGGTAAAAAAATGCCTGGACGCATGGGTGGGAAGCAGAGAACTGTGAAAAATGTGTGGGTCTACAAAATTGACCCCGCAAGAAACTTGATATGGGTGAAAGGCCAAGTACGTTTCTTATAAAAACCTAATTCCAACTCCTGATCCAATCCATATTCTCTAGAGTCCTTGTTCTGTTTCTATGTTTGACCCATAAATTTCATACTCTTGGCACAACATGGTCTGAGATTATTGCTGCACACACCTTCAGGTCCCCGGTGCCGAAGGAAACTTTGTATTTATAAAAGACGCATTCTACAAGAAACCCGATAAGTCGATCCTTCCGTTTCCCACCTACTTCGCAACTGAAGACGAGGACTCCGAGAAGTTGGAACCTCTTGTTGCTGATCTTGGAGAAGTAGATCCATTTATGGTTGGAGATTGATTAGGAGTTGCCTTTTCAGCGCAAGTAGTGGATTTTGGTCTGCTTTTGGTTTTTTATTTTCAACTTCTGATAGTGTATGCAGGCGTGCTTATTGATGGCCCTGATGAGTAGTCAATTTCATTATAGAAATGCAGCTGGAGGATCTGTACGAGGAAATCCAACCTCTTAATGCGACGGTAATTTACCAATTTTTTTTCCCCTTTTCCCCCTTTTTCCTATTTGTTTATTTACAACCGCCATCTCAAACATGTACCTGAAATAGCCCTTTTGAAATAAAGAGTAGAGAAGAGGGAGGCTATAAATATTGAGAATTTTTGTTGAGTGGTTTTAACTTTTTATCCTTTTTGTTTATGTGAACTTGGAAAATAGATTGTTTATGGCTGCCATAGTTAGTAATATCTTAACTACCCGAGCGGTATCTCTTATGGGTTGGAAGTTCAAATCTCGTTCTTTTATTTGTAATGTAATATTCTTAAGG

mRNA sequence

ATGTCGGCTTTATCCAGAGGTCTCGTTTCTCGTTTCCGTCTTCTCTCCATCAATTCCGGCGCCTCTTCTACAATTTCCCCTTCTTCTTCGCGAACGCATCCCTCTTGTTACTACTTCTTCCGTTCTTTCAGCTCCGAGGATTTGATCCAGGCCGGCGACGTCTCCTCGCCTTCTTCTCCGATCATTGAAGCTAAGCCCGGTGTCATGGCCCCTAACTCGAAGCGGACGGGCGTCATTGCCGTCAAGTGTGGAATGTCGGCGCTGTGGGATAAATGGGGAGCTAGGATACCCATCACCGTGCTCTGGGTTGATGATAATATTGTGTCTCAGGTCAAGACAATCGAGAAAGAAGGTATCACAGCCCTCCAGATTGGCTGTGGACAGAAGAAGGCGAAGCATTTGACAAAACCGGAATTAGGACATTTTAGGGCTCAAGGTGTTCCATTGAAGAGGAAACTGAGGGAGTTTCCAGTGTCAGCAGATGCACTTCTTCCAGTTGGTACAACAATTGGTGTTCGTCATTTTGTCCCTGGACAGTATGTTGATGTGACTGGAATCACAAGAGGGAAAGGTTTTCAGGGAGTGATGAAAAGGCATGGATTTAAAGGAATGCCAGCATCCCATGGAGCATCATTATCCCATAGAAGCCCTGGTTCTACGGGTCAGAGGGATGCTCCTGGAAAGGTCTTTAAAGGTAAAAAAATGCCTGGACGCATGGGTGGGAAGCAGAGAACTGTGAAAAATGTGTGGGTCTACAAAATTGACCCCGCAAGAAACTTGATATGGGTGAAAGGCCAAGTCCCCGGTGCCGAAGGAAACTTTGTATTTATAAAAGACGCATTCTACAAGAAACCCGATAAGTCGATCCTTCCGTTTCCCACCTACTTCGCAACTGAAGACGAGGACTCCGAGAAGTTGGAACCTCTTGTTGCTGATCTTGGAGAAGTAGATCCATTTATGGTTGGAGATTGATTAGGAGTTGCCTTTTCAGCGCAAGTAGTGGATTTTGGTCTGCTTTTGGTTTTTTATTTTCAACTTCTGATAGTGTATGCAGGCGTGCTTATTGATGGCCCTGATGAGTAGTCAATTTCATTATAGAAATGCAGCTGGAGGATCTGTACGAGGAAATCCAACCTCTTAATGCGACGGTAATTTACCAATTTTTTTTCCCCTTTTCCCCCTTTTTCCTATTTGTTTATTTACAACCGCCATCTCAAACATGTACCTGAAATAGCCCTTTTGAAATAAAGAGTAGAGAAGAGGGAGGCTATAAATATTGAGAATTTTTGTTGAGTGGTTTTAACTTTTTATCCTTTTTGTTTATGTGAACTTGGAAAATAGATTGTTTATGGCTGCCATAGTTAGTAATATCTTAACTACCCGAGCGGTATCTCTTATGGGTTGGAAGTTCAAATCTCGTTCTTTTATTTGTAATGTAATATTCTTAAGG

Coding sequence (CDS)

ATGTCGGCTTTATCCAGAGGTCTCGTTTCTCGTTTCCGTCTTCTCTCCATCAATTCCGGCGCCTCTTCTACAATTTCCCCTTCTTCTTCGCGAACGCATCCCTCTTGTTACTACTTCTTCCGTTCTTTCAGCTCCGAGGATTTGATCCAGGCCGGCGACGTCTCCTCGCCTTCTTCTCCGATCATTGAAGCTAAGCCCGGTGTCATGGCCCCTAACTCGAAGCGGACGGGCGTCATTGCCGTCAAGTGTGGAATGTCGGCGCTGTGGGATAAATGGGGAGCTAGGATACCCATCACCGTGCTCTGGGTTGATGATAATATTGTGTCTCAGGTCAAGACAATCGAGAAAGAAGGTATCACAGCCCTCCAGATTGGCTGTGGACAGAAGAAGGCGAAGCATTTGACAAAACCGGAATTAGGACATTTTAGGGCTCAAGGTGTTCCATTGAAGAGGAAACTGAGGGAGTTTCCAGTGTCAGCAGATGCACTTCTTCCAGTTGGTACAACAATTGGTGTTCGTCATTTTGTCCCTGGACAGTATGTTGATGTGACTGGAATCACAAGAGGGAAAGGTTTTCAGGGAGTGATGAAAAGGCATGGATTTAAAGGAATGCCAGCATCCCATGGAGCATCATTATCCCATAGAAGCCCTGGTTCTACGGGTCAGAGGGATGCTCCTGGAAAGGTCTTTAAAGGTAAAAAAATGCCTGGACGCATGGGTGGGAAGCAGAGAACTGTGAAAAATGTGTGGGTCTACAAAATTGACCCCGCAAGAAACTTGATATGGGTGAAAGGCCAAGTCCCCGGTGCCGAAGGAAACTTTGTATTTATAAAAGACGCATTCTACAAGAAACCCGATAAGTCGATCCTTCCGTTTCCCACCTACTTCGCAACTGAAGACGAGGACTCCGAGAAGTTGGAACCTCTTGTTGCTGATCTTGGAGAAGTAGATCCATTTATGGTTGGAGATTGA

Protein sequence

MSALSRGLVSRFRLLSINSGASSTISPSSSRTHPSCYYFFRSFSSEDLIQAGDVSSPSSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFATEDEDSEKLEPLVADLGEVDPFMVGD
Homology
BLAST of MC05g0709 vs. ExPASy Swiss-Prot
Match: Q9LRN8 (50S ribosomal protein L3-2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RPL3B PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.4e-135
Identity = 247/329 (75.08%), Postives = 275/329 (83.59%), Query Frame = 0

Query: 1   MSALSRGLVSRF-RLLSINSGASSTISPSSSRTHPSCYYFF--RSFSSEDLIQAG---DV 60
           M+A+ RGL+SR  + LSI S     I+PSSS + P C  FF  R FSS+  +  G   D+
Sbjct: 1   MAAVPRGLLSRINQFLSIRS-----ITPSSSESLPHCSSFFLIRRFSSDTGLMDGGGSDI 60

Query: 61  SSPSSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTI 120
               + IIEAK G M+  SKRTG+IAVKCGM+ALWDKWG RIPI++LWVDDNIVSQVKT+
Sbjct: 61  IGAQTRIIEAKQGEMSSRSKRTGIIAVKCGMTALWDKWGKRIPISILWVDDNIVSQVKTV 120

Query: 121 EKEGITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRH 180
           EKEGI ALQIGCGQKK KHL+K  +GHFRAQGVPLKRKLREFPV+ DALLPVGT++GVRH
Sbjct: 121 EKEGIFALQIGCGQKKPKHLSKAVVGHFRAQGVPLKRKLREFPVTEDALLPVGTSLGVRH 180

Query: 181 FVPGQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKK 240
           FVPGQYVDVTGITRGKGFQG MKRHGF GMPASHGASLSHRS GSTGQRDAPGKVFKG+K
Sbjct: 181 FVPGQYVDVTGITRGKGFQGCMKRHGFSGMPASHGASLSHRSGGSTGQRDAPGKVFKGRK 240

Query: 241 MPGRMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPT 300
           M GRMG  QRTVKNVWVYKIDPARNL+WV+GQVPGAEGNFVFIKDA+ KKPD S LPFPT
Sbjct: 241 MAGRMGADQRTVKNVWVYKIDPARNLMWVRGQVPGAEGNFVFIKDAWCKKPDISKLPFPT 300

Query: 301 YFATEDEDSEKLEPLVADLGEVDPFMVGD 324
           Y A EDED  +LEPLVADLGEVDPFM+ +
Sbjct: 301 YLAPEDEDPSELEPLVADLGEVDPFMLAE 324

BLAST of MC05g0709 vs. ExPASy Swiss-Prot
Match: Q2G8Y0 (50S ribosomal protein L3 OS=Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199) OX=279238 GN=rplC PE=3 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 9.7e-58
Identity = 111/206 (53.88%), Postives = 148/206 (71.84%), Query Frame = 0

Query: 75  RTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGITALQIGCGQKKAKHL 134
           RTGVIA K GM+ L+ + G  +P+TVL +++  V  V+T E++G  ALQ+G G+ K K++
Sbjct: 2   RTGVIAKKVGMTRLFQEDGRHVPVTVLSLENCQVVSVRTAERDGYVALQLGAGEAKQKNV 61

Query: 135 TKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQYVDVTGITRGKGFQG 194
            KP+  HF    VPLK ++ EF V+ DALL VG+TI   HFVPGQYVD+TG T+GKGFQG
Sbjct: 62  AKPQREHFAKAEVPLKMEVAEFRVADDALLDVGSTIAASHFVPGQYVDITGQTQGKGFQG 121

Query: 195 VMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMGGKQRTVKNVWVYKI 254
            MKR GF GM A+HG S+SHR+ GSTG R  PG+VFK KKM G MG +QRT +N+ V + 
Sbjct: 122 AMKRWGFGGMRATHGVSISHRAHGSTGNRQDPGRVFKNKKMAGHMGDRQRTQQNLEVVRT 181

Query: 255 DPARNLIWVKGQVPGAEGNFVFIKDA 281
           D  R LI+VKG VPG++  ++ ++DA
Sbjct: 182 DDERGLIFVKGSVPGSKNAWLLVRDA 207

BLAST of MC05g0709 vs. ExPASy Swiss-Prot
Match: Q5NQ64 (50S ribosomal protein L3 OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) OX=264203 GN=rplC PE=3 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.7e-57
Identity = 117/238 (49.16%), Postives = 157/238 (65.97%), Query Frame = 0

Query: 75  RTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGITALQIGCGQKKAKHL 134
           RTGVIA K GM+ L+ + G  +P+TVL ++D  V  VK  +++G  A+Q+G G  KAK++
Sbjct: 2   RTGVIAKKVGMTRLFQEDGRHVPVTVLKLEDVQVVAVKNKDQDGYVAVQLGAGSAKAKNV 61

Query: 135 TKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQYVDVTGITRGKGFQG 194
           TKP  GHF    V LK KL EFPVS DA+L VGT+I   HF+ GQ VD++G T+GKGF G
Sbjct: 62  TKPVRGHFAKAEVELKAKLVEFPVSEDAVLEVGTSISADHFIAGQLVDISGQTQGKGFAG 121

Query: 195 VMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMGGKQRTVKNVWVYKI 254
            MKR GF G+ A+HG SLSHRS GSTG R  PG+VFK KKM G MG +QRT +N+ V   
Sbjct: 122 AMKRWGFGGLRATHGVSLSHRSHGSTGNRQDPGRVFKNKKMAGHMGARQRTQQNLEVIST 181

Query: 255 DPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFATEDEDSEKLEPLVAD 313
           D  R+LI+V+G VPG++G ++ I+DA  K    +  P+P      + +     P+ A+
Sbjct: 182 DAERDLIFVRGSVPGSKGAWLIIRDA-VKVARPTDAPYPAAIKAANGNEAPAAPVAAE 238

BLAST of MC05g0709 vs. ExPASy Swiss-Prot
Match: Q5FTY3 (50S ribosomal protein L3 OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=rplC PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.4e-56
Identity = 115/219 (52.51%), Postives = 146/219 (66.67%), Query Frame = 0

Query: 75  RTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGITALQIGCGQKKAKHL 134
           RTG+IA K GM+ L+ + G  +P+TVL +D+  V   +T E++G TA+Q+G G  K K++
Sbjct: 2   RTGLIAKKLGMTRLFKEDGTHVPVTVLHLDNVEVVDARTNERDGYTAIQLGLGNAKVKNV 61

Query: 135 TKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQYVDVTGITRGKGFQG 194
           TK   GHF    V  KR L EF VS DALL  GT +   HFV GQ VDVTG ++GKGF G
Sbjct: 62  TKANRGHFARTKVEPKRHLAEFRVSEDALLEAGTKLSAAHFVVGQKVDVTGQSKGKGFAG 121

Query: 195 VMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMGGKQRTVKNVWVYKI 254
           VMKRH F G+ ASHG S+SHRS GSTGQR  PGKVFKGKKM G MG ++ T  N+ +  +
Sbjct: 122 VMKRHNFAGLEASHGVSISHRSHGSTGQRQDPGKVFKGKKMAGHMGDERVTTLNLEIAAV 181

Query: 255 DPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFP 294
           D  RNLI V+G +PGA+   V I+DA  KK   +  P+P
Sbjct: 182 DEERNLIMVRGAIPGAKNGLVLIRDAI-KKARHADAPYP 219

BLAST of MC05g0709 vs. ExPASy Swiss-Prot
Match: Q5LLU6 (50S ribosomal protein L3 OS=Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) OX=246200 GN=rplC PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.6e-55
Identity = 115/219 (52.51%), Postives = 147/219 (67.12%), Query Frame = 0

Query: 75  RTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGITALQIGCGQKKAKHL 134
           R+G+IA K GM+ L+ + G +IP+TVL +D+  V   +T EK+G TA+Q+G G  K K +
Sbjct: 2   RSGIIAKKVGMTRLFMEDGKQIPVTVLQLDNLQVVAQRTTEKDGYTAVQLGAGTPKIKRV 61

Query: 135 TKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQYVDVTGITRGKGFQG 194
           +K   GHF AQ V  KRKL EF VS DAL+ VG  I   HF+ GQ VDV+G + GKGF G
Sbjct: 62  SKAMRGHFAAQKVEPKRKLAEFRVSEDALIEVGAEISAEHFLTGQKVDVSGTSIGKGFAG 121

Query: 195 VMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMGGKQRTVKNVWVYKI 254
            MKRH F G+ ASHG S+SHRS GSTGQ   PG+VFKGKKM G MG  + T +N+ V K 
Sbjct: 122 AMKRHNFGGLRASHGVSISHRSHGSTGQCQDPGRVFKGKKMAGHMGAARVTTQNLEVVKT 181

Query: 255 DPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFP 294
           D  R L+++KG VPG +  +V +KDA  KK  +  LPFP
Sbjct: 182 DAERGLVFIKGAVPGPKSGWVTVKDAVKKKAPEG-LPFP 219

BLAST of MC05g0709 vs. NCBI nr
Match: XP_022138212.1 (50S ribosomal protein L3-2, chloroplastic [Momordica charantia])

HSP 1 Score: 647 bits (1669), Expect = 3.27e-234
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MSALSRGLVSRFRLLSINSGASSTISPSSSRTHPSCYYFFRSFSSEDLIQAGDVSSPSSP 60
           MSALSRGLVSRFRLLSINSGASSTISPSSSRTHPSCYYFFRSFSSEDLIQAGDVSSPSSP
Sbjct: 1   MSALSRGLVSRFRLLSINSGASSTISPSSSRTHPSCYYFFRSFSSEDLIQAGDVSSPSSP 60

Query: 61  IIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGIT 120
           IIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGIT
Sbjct: 61  IIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGIT 120

Query: 121 ALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQY 180
           ALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQY
Sbjct: 121 ALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQY 180

Query: 181 VDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMG 240
           VDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMG
Sbjct: 181 VDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMG 240

Query: 241 GKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFATED 300
           GKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFATED
Sbjct: 241 GKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFATED 300

Query: 301 EDSEKLEPLVADLGEVDPFMVGD 323
           EDSEKLEPLVADLGEVDPFMVGD
Sbjct: 301 EDSEKLEPLVADLGEVDPFMVGD 323

BLAST of MC05g0709 vs. NCBI nr
Match: XP_022972230.1 (50S ribosomal protein L3-2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 596 bits (1537), Expect = 4.82e-214
Identity = 299/326 (91.72%), Postives = 311/326 (95.40%), Query Frame = 0

Query: 1   MSALSRGLVSRFRLLSINSGASSTI---SPSSSRTHPSCYYFFRSFSSEDLIQAGDVSSP 60
           MSALSRGL+SRFRLLSINSGAS TI   S SSS THPSCY+FFR+FS+E  IQAGD SS 
Sbjct: 1   MSALSRGLISRFRLLSINSGASPTITSSSSSSSWTHPSCYFFFRTFSTEGSIQAGDGSSL 60

Query: 61  SSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKE 120
           SSPIIEAK GVM+PNSKRTGVIAVKCGMSALWDKWGAR+PITVLWVDDNIVSQVKTIEKE
Sbjct: 61  SSPIIEAKSGVMSPNSKRTGVIAVKCGMSALWDKWGARVPITVLWVDDNIVSQVKTIEKE 120

Query: 121 GITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVP 180
           GITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVS DALLPVGTTIGVRHFVP
Sbjct: 121 GITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSEDALLPVGTTIGVRHFVP 180

Query: 181 GQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPG 240
           GQ+VDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKM G
Sbjct: 181 GQFVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMAG 240

Query: 241 RMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFA 300
           RMGGKQRTVKNVWVYKIDPARNL+WV+GQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFA
Sbjct: 241 RMGGKQRTVKNVWVYKIDPARNLMWVRGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFA 300

Query: 301 TEDEDSEKLEPLVADLGEVDPFMVGD 323
            +DED+E LEPLVADLGEVDPFMVG+
Sbjct: 301 NDDEDTETLEPLVADLGEVDPFMVGE 326

BLAST of MC05g0709 vs. NCBI nr
Match: XP_022947107.1 (50S ribosomal protein L3-2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 588 bits (1516), Expect = 7.92e-211
Identity = 294/327 (89.91%), Postives = 309/327 (94.50%), Query Frame = 0

Query: 1   MSALSRGLVSRFRLLSINSGASSTI----SPSSSRTHPSCYYFFRSFSSEDLIQAGDVSS 60
           MSALSRGL+SRFRLLSINS AS TI    S SSS THPSCY+FFR+FS+E  IQAGD SS
Sbjct: 1   MSALSRGLISRFRLLSINSAASPTITSSSSSSSSWTHPSCYFFFRTFSTEGSIQAGDGSS 60

Query: 61  PSSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEK 120
            SSPIIEAKPGVM+PNSKRTGVIAVKCGMSALWDKWGAR+PITVLWVDDNIVSQVKTIEK
Sbjct: 61  LSSPIIEAKPGVMSPNSKRTGVIAVKCGMSALWDKWGARVPITVLWVDDNIVSQVKTIEK 120

Query: 121 EGITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFV 180
           EGITALQIGCGQKKAKHLTKPE+GHFRAQGVPLKRKLREFPV+ DALLPVGTTIGVRHFV
Sbjct: 121 EGITALQIGCGQKKAKHLTKPEVGHFRAQGVPLKRKLREFPVTEDALLPVGTTIGVRHFV 180

Query: 181 PGQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMP 240
           PGQ++DVTGITRGKGFQGVMK+HGFKGMPASHGASLSHRS GSTGQRDAPGKVFKGKKM 
Sbjct: 181 PGQFIDVTGITRGKGFQGVMKKHGFKGMPASHGASLSHRSLGSTGQRDAPGKVFKGKKMA 240

Query: 241 GRMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYF 300
           GRMGGKQRTVKNVWVYKIDPARNL+WV+GQVPGAEGNFVFIKDAFYKKPDKSILPFPTYF
Sbjct: 241 GRMGGKQRTVKNVWVYKIDPARNLMWVRGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYF 300

Query: 301 ATEDEDSEKLEPLVADLGEVDPFMVGD 323
           A  DED+E LEPLVADLGEVDPFMVG+
Sbjct: 301 ANNDEDTETLEPLVADLGEVDPFMVGE 327

BLAST of MC05g0709 vs. NCBI nr
Match: KAG7029037.1 (50S ribosomal protein L3-2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587 bits (1513), Expect = 2.27e-210
Identity = 293/327 (89.60%), Postives = 309/327 (94.50%), Query Frame = 0

Query: 1   MSALSRGLVSRFRLLSINSGASSTI----SPSSSRTHPSCYYFFRSFSSEDLIQAGDVSS 60
           MSALSRGL+SRFRLLSINS AS TI    S SSS THPSCY+FFR+FS+E  IQAGD SS
Sbjct: 1   MSALSRGLISRFRLLSINSAASPTITSSSSSSSSWTHPSCYFFFRTFSTEGSIQAGDGSS 60

Query: 61  PSSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEK 120
            +SPIIEAKPGVM+PNSKRTGVIAVKCGMSALWDKWGAR+PITVLWVDDNIVSQVKTIEK
Sbjct: 61  LTSPIIEAKPGVMSPNSKRTGVIAVKCGMSALWDKWGARVPITVLWVDDNIVSQVKTIEK 120

Query: 121 EGITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFV 180
           EGITALQIGCGQKKAKHLTKPE+GHFRAQGVPLKRKLREFPV+ DALLPVGTTIGVRHFV
Sbjct: 121 EGITALQIGCGQKKAKHLTKPEVGHFRAQGVPLKRKLREFPVTEDALLPVGTTIGVRHFV 180

Query: 181 PGQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMP 240
           PGQ++DVTGITRGKGFQGVMK+HGFKGMPASHGASLSHRS GSTGQRDAPGKVFKGKKM 
Sbjct: 181 PGQFIDVTGITRGKGFQGVMKKHGFKGMPASHGASLSHRSLGSTGQRDAPGKVFKGKKMA 240

Query: 241 GRMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYF 300
           GRMGGKQRTVKNVWVYKIDPARNL+WV+GQVPGAEGNFVFIKDAFYKKPDKSILPFPTYF
Sbjct: 241 GRMGGKQRTVKNVWVYKIDPARNLMWVRGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYF 300

Query: 301 ATEDEDSEKLEPLVADLGEVDPFMVGD 323
           A  DED+E LEPLVADLGEVDPFMVG+
Sbjct: 301 ANNDEDTETLEPLVADLGEVDPFMVGE 327

BLAST of MC05g0709 vs. NCBI nr
Match: KAG6597593.1 (50S ribosomal protein L3-2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 586 bits (1511), Expect = 4.58e-210
Identity = 294/327 (89.91%), Postives = 309/327 (94.50%), Query Frame = 0

Query: 1   MSALSRGLVSRFRLLSINSGASSTI----SPSSSRTHPSCYYFFRSFSSEDLIQAGDVSS 60
           MSALSRGL+SRFRLLSINS AS TI    S SSS THPSCY+FFR+FS+E  IQAGD SS
Sbjct: 1   MSALSRGLISRFRLLSINSAASPTITSSSSSSSSWTHPSCYFFFRTFSTEGSIQAGDGSS 60

Query: 61  PSSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEK 120
            SSPIIEAKPGVM+PNSKRTGVIAVKCGMSALWDKWGAR+PITVLWVDDNIVSQVKTIEK
Sbjct: 61  LSSPIIEAKPGVMSPNSKRTGVIAVKCGMSALWDKWGARVPITVLWVDDNIVSQVKTIEK 120

Query: 121 EGITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFV 180
           EGITALQIGCGQKKAKHLTKPE+GHFRAQGVPLKRKLREFPV+ DALLPVGTTIGVRHFV
Sbjct: 121 EGITALQIGCGQKKAKHLTKPEVGHFRAQGVPLKRKLREFPVTEDALLPVGTTIGVRHFV 180

Query: 181 PGQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMP 240
           PGQ+VDVTGITRGKGFQGVMKRHGF GMPASHGASLSHRS GSTGQRDAPGKVFKGKKM 
Sbjct: 181 PGQFVDVTGITRGKGFQGVMKRHGFDGMPASHGASLSHRSLGSTGQRDAPGKVFKGKKMA 240

Query: 241 GRMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYF 300
           GRMGGKQRTVKNVWVYKIDPARNL+WV+GQVPGAEGNFVFIKDAFYKKP+KSILPFPTYF
Sbjct: 241 GRMGGKQRTVKNVWVYKIDPARNLMWVRGQVPGAEGNFVFIKDAFYKKPNKSILPFPTYF 300

Query: 301 ATEDEDSEKLEPLVADLGEVDPFMVGD 323
           A +DED+E LEPLVADLGEVDPFMVG+
Sbjct: 301 ANDDEDTETLEPLVADLGEVDPFMVGE 327

BLAST of MC05g0709 vs. ExPASy TrEMBL
Match: A0A6J1CAF7 (50S ribosomal protein L3-2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111009440 PE=3 SV=1)

HSP 1 Score: 647 bits (1669), Expect = 1.58e-234
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MSALSRGLVSRFRLLSINSGASSTISPSSSRTHPSCYYFFRSFSSEDLIQAGDVSSPSSP 60
           MSALSRGLVSRFRLLSINSGASSTISPSSSRTHPSCYYFFRSFSSEDLIQAGDVSSPSSP
Sbjct: 1   MSALSRGLVSRFRLLSINSGASSTISPSSSRTHPSCYYFFRSFSSEDLIQAGDVSSPSSP 60

Query: 61  IIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGIT 120
           IIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGIT
Sbjct: 61  IIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKEGIT 120

Query: 121 ALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQY 180
           ALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQY
Sbjct: 121 ALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVPGQY 180

Query: 181 VDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMG 240
           VDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMG
Sbjct: 181 VDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMG 240

Query: 241 GKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFATED 300
           GKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFATED
Sbjct: 241 GKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFATED 300

Query: 301 EDSEKLEPLVADLGEVDPFMVGD 323
           EDSEKLEPLVADLGEVDPFMVGD
Sbjct: 301 EDSEKLEPLVADLGEVDPFMVGD 323

BLAST of MC05g0709 vs. ExPASy TrEMBL
Match: A0A6J1I7Y9 (50S ribosomal protein L3-2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470821 PE=3 SV=1)

HSP 1 Score: 596 bits (1537), Expect = 2.33e-214
Identity = 299/326 (91.72%), Postives = 311/326 (95.40%), Query Frame = 0

Query: 1   MSALSRGLVSRFRLLSINSGASSTI---SPSSSRTHPSCYYFFRSFSSEDLIQAGDVSSP 60
           MSALSRGL+SRFRLLSINSGAS TI   S SSS THPSCY+FFR+FS+E  IQAGD SS 
Sbjct: 1   MSALSRGLISRFRLLSINSGASPTITSSSSSSSWTHPSCYFFFRTFSTEGSIQAGDGSSL 60

Query: 61  SSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEKE 120
           SSPIIEAK GVM+PNSKRTGVIAVKCGMSALWDKWGAR+PITVLWVDDNIVSQVKTIEKE
Sbjct: 61  SSPIIEAKSGVMSPNSKRTGVIAVKCGMSALWDKWGARVPITVLWVDDNIVSQVKTIEKE 120

Query: 121 GITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFVP 180
           GITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVS DALLPVGTTIGVRHFVP
Sbjct: 121 GITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSEDALLPVGTTIGVRHFVP 180

Query: 181 GQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPG 240
           GQ+VDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKM G
Sbjct: 181 GQFVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMAG 240

Query: 241 RMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFA 300
           RMGGKQRTVKNVWVYKIDPARNL+WV+GQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFA
Sbjct: 241 RMGGKQRTVKNVWVYKIDPARNLMWVRGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYFA 300

Query: 301 TEDEDSEKLEPLVADLGEVDPFMVGD 323
            +DED+E LEPLVADLGEVDPFMVG+
Sbjct: 301 NDDEDTETLEPLVADLGEVDPFMVGE 326

BLAST of MC05g0709 vs. ExPASy TrEMBL
Match: A0A6J1G5I5 (50S ribosomal protein L3-2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451076 PE=3 SV=1)

HSP 1 Score: 588 bits (1516), Expect = 3.84e-211
Identity = 294/327 (89.91%), Postives = 309/327 (94.50%), Query Frame = 0

Query: 1   MSALSRGLVSRFRLLSINSGASSTI----SPSSSRTHPSCYYFFRSFSSEDLIQAGDVSS 60
           MSALSRGL+SRFRLLSINS AS TI    S SSS THPSCY+FFR+FS+E  IQAGD SS
Sbjct: 1   MSALSRGLISRFRLLSINSAASPTITSSSSSSSSWTHPSCYFFFRTFSTEGSIQAGDGSS 60

Query: 61  PSSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEK 120
            SSPIIEAKPGVM+PNSKRTGVIAVKCGMSALWDKWGAR+PITVLWVDDNIVSQVKTIEK
Sbjct: 61  LSSPIIEAKPGVMSPNSKRTGVIAVKCGMSALWDKWGARVPITVLWVDDNIVSQVKTIEK 120

Query: 121 EGITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFV 180
           EGITALQIGCGQKKAKHLTKPE+GHFRAQGVPLKRKLREFPV+ DALLPVGTTIGVRHFV
Sbjct: 121 EGITALQIGCGQKKAKHLTKPEVGHFRAQGVPLKRKLREFPVTEDALLPVGTTIGVRHFV 180

Query: 181 PGQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMP 240
           PGQ++DVTGITRGKGFQGVMK+HGFKGMPASHGASLSHRS GSTGQRDAPGKVFKGKKM 
Sbjct: 181 PGQFIDVTGITRGKGFQGVMKKHGFKGMPASHGASLSHRSLGSTGQRDAPGKVFKGKKMA 240

Query: 241 GRMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYF 300
           GRMGGKQRTVKNVWVYKIDPARNL+WV+GQVPGAEGNFVFIKDAFYKKPDKSILPFPTYF
Sbjct: 241 GRMGGKQRTVKNVWVYKIDPARNLMWVRGQVPGAEGNFVFIKDAFYKKPDKSILPFPTYF 300

Query: 301 ATEDEDSEKLEPLVADLGEVDPFMVGD 323
           A  DED+E LEPLVADLGEVDPFMVG+
Sbjct: 301 ANNDEDTETLEPLVADLGEVDPFMVGE 327

BLAST of MC05g0709 vs. ExPASy TrEMBL
Match: E5GC40 (50S ribosomal protein l3 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 556 bits (1432), Expect = 2.50e-198
Identity = 278/328 (84.76%), Postives = 302/328 (92.07%), Query Frame = 0

Query: 1   MSALSRGLVSRFRLLSINSGASSTISPSS----SRTHPSCYYFFRSFSSEDLIQAGDVSS 60
           MSALSRGL+SR RLLSINSGAS +++ SS    SRT+ SCYYFFR+FS++DL QA D S 
Sbjct: 1   MSALSRGLISRLRLLSINSGASHSVTSSSYSSSSRTNASCYYFFRAFSTQDLTQASDGSL 60

Query: 61  PSSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEK 120
           P+S IIEAKPG M+ NS+RTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEK
Sbjct: 61  PASSIIEAKPGTMSSNSRRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEK 120

Query: 121 EGITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFV 180
           EGITALQIGCGQKK KHL+KPELGHFRAQGVPLKRKL+EFPVS DALLPVGT IG RHFV
Sbjct: 121 EGITALQIGCGQKKEKHLSKPELGHFRAQGVPLKRKLKEFPVSQDALLPVGTEIGARHFV 180

Query: 181 PGQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMP 240
           PGQYVDVTGI+RGKGFQGVMKRHGFKGMPASHGASLSHRS GSTGQRDAPGKVFKG+KM 
Sbjct: 181 PGQYVDVTGISRGKGFQGVMKRHGFKGMPASHGASLSHRSGGSTGQRDAPGKVFKGRKMA 240

Query: 241 GRMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKS-ILPFPTY 300
           GRMGGKQRTVKNVW+YKIDPARNLIWVKG +PGAEG+FVFIKDA YK+ +KS ILPFPTY
Sbjct: 241 GRMGGKQRTVKNVWIYKIDPARNLIWVKGPIPGAEGSFVFIKDAMYKRLNKSAILPFPTY 300

Query: 301 FATEDEDSEKLEPLVADLGEVDPFMVGD 323
           FAT+ ED++KLEPLVADLGEVDPFM+GD
Sbjct: 301 FATDGEDTDKLEPLVADLGEVDPFMIGD 328

BLAST of MC05g0709 vs. ExPASy TrEMBL
Match: A0A1S3AYD1 (50S ribosomal protein L3-2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483925 PE=3 SV=1)

HSP 1 Score: 556 bits (1432), Expect = 2.50e-198
Identity = 278/328 (84.76%), Postives = 302/328 (92.07%), Query Frame = 0

Query: 1   MSALSRGLVSRFRLLSINSGASSTISPSS----SRTHPSCYYFFRSFSSEDLIQAGDVSS 60
           MSALSRGL+SR RLLSINSGAS +++ SS    SRT+ SCYYFFR+FS++DL QA D S 
Sbjct: 1   MSALSRGLISRLRLLSINSGASHSVTSSSYSSSSRTNASCYYFFRAFSTQDLTQASDGSL 60

Query: 61  PSSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEK 120
           P+S IIEAKPG M+ NS+RTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEK
Sbjct: 61  PASSIIEAKPGTMSSNSRRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTIEK 120

Query: 121 EGITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRHFV 180
           EGITALQIGCGQKK KHL+KPELGHFRAQGVPLKRKL+EFPVS DALLPVGT IG RHFV
Sbjct: 121 EGITALQIGCGQKKEKHLSKPELGHFRAQGVPLKRKLKEFPVSQDALLPVGTEIGARHFV 180

Query: 181 PGQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMP 240
           PGQYVDVTGI+RGKGFQGVMKRHGFKGMPASHGASLSHRS GSTGQRDAPGKVFKG+KM 
Sbjct: 181 PGQYVDVTGISRGKGFQGVMKRHGFKGMPASHGASLSHRSGGSTGQRDAPGKVFKGRKMA 240

Query: 241 GRMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKS-ILPFPTY 300
           GRMGGKQRTVKNVW+YKIDPARNLIWVKG +PGAEG+FVFIKDA YK+ +KS ILPFPTY
Sbjct: 241 GRMGGKQRTVKNVWIYKIDPARNLIWVKGPIPGAEGSFVFIKDAMYKRLNKSAILPFPTY 300

Query: 301 FATEDEDSEKLEPLVADLGEVDPFMVGD 323
           FAT+ ED++KLEPLVADLGEVDPFM+GD
Sbjct: 301 FATDGEDTDKLEPLVADLGEVDPFMIGD 328

BLAST of MC05g0709 vs. TAIR 10
Match: AT3G17465.1 (ribosomal protein L3 plastid )

HSP 1 Score: 482.3 bits (1240), Expect = 3.1e-136
Identity = 247/329 (75.08%), Postives = 275/329 (83.59%), Query Frame = 0

Query: 1   MSALSRGLVSRF-RLLSINSGASSTISPSSSRTHPSCYYFF--RSFSSEDLIQAG---DV 60
           M+A+ RGL+SR  + LSI S     I+PSSS + P C  FF  R FSS+  +  G   D+
Sbjct: 1   MAAVPRGLLSRINQFLSIRS-----ITPSSSESLPHCSSFFLIRRFSSDTGLMDGGGSDI 60

Query: 61  SSPSSPIIEAKPGVMAPNSKRTGVIAVKCGMSALWDKWGARIPITVLWVDDNIVSQVKTI 120
               + IIEAK G M+  SKRTG+IAVKCGM+ALWDKWG RIPI++LWVDDNIVSQVKT+
Sbjct: 61  IGAQTRIIEAKQGEMSSRSKRTGIIAVKCGMTALWDKWGKRIPISILWVDDNIVSQVKTV 120

Query: 121 EKEGITALQIGCGQKKAKHLTKPELGHFRAQGVPLKRKLREFPVSADALLPVGTTIGVRH 180
           EKEGI ALQIGCGQKK KHL+K  +GHFRAQGVPLKRKLREFPV+ DALLPVGT++GVRH
Sbjct: 121 EKEGIFALQIGCGQKKPKHLSKAVVGHFRAQGVPLKRKLREFPVTEDALLPVGTSLGVRH 180

Query: 181 FVPGQYVDVTGITRGKGFQGVMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKK 240
           FVPGQYVDVTGITRGKGFQG MKRHGF GMPASHGASLSHRS GSTGQRDAPGKVFKG+K
Sbjct: 181 FVPGQYVDVTGITRGKGFQGCMKRHGFSGMPASHGASLSHRSGGSTGQRDAPGKVFKGRK 240

Query: 241 MPGRMGGKQRTVKNVWVYKIDPARNLIWVKGQVPGAEGNFVFIKDAFYKKPDKSILPFPT 300
           M GRMG  QRTVKNVWVYKIDPARNL+WV+GQVPGAEGNFVFIKDA+ KKPD S LPFPT
Sbjct: 241 MAGRMGADQRTVKNVWVYKIDPARNLMWVRGQVPGAEGNFVFIKDAWCKKPDISKLPFPT 300

Query: 301 YFATEDEDSEKLEPLVADLGEVDPFMVGD 324
           Y A EDED  +LEPLVADLGEVDPFM+ +
Sbjct: 301 YLAPEDEDPSELEPLVADLGEVDPFMLAE 324

BLAST of MC05g0709 vs. TAIR 10
Match: AT2G43030.1 (Ribosomal protein L3 family protein )

HSP 1 Score: 154.5 bits (389), Expect = 1.5e-37
Identity = 86/206 (41.75%), Postives = 125/206 (60.68%), Query Frame = 0

Query: 77  GVIAVKCGMSALWDKWGARIPITVL-WVDDNIVSQVKTIEKEGITALQIGCGQKKAKHLT 136
           GV+  K GM + +++ G  +P+TV+ + + NIV+Q+KT+  +G  A+QIG  + + K LT
Sbjct: 57  GVMGSKLGMMSFFEEDGTVVPVTVVGFREGNIVTQIKTLATDGYDAVQIGYRRVRDKKLT 116

Query: 137 KPELGHFRAQGVPLKRKLREFPV-SADALLPVGTTIGVRHFVPGQYVDVTGITRGKGFQG 196
           KPE GH +  G    R L+EF + + +   P    +    F  G  VDV G T GKGFQG
Sbjct: 117 KPETGHLQKAGTIPMRHLQEFRLTNIEGFEPNQKLVFDEIFKEGDLVDVAGTTIGKGFQG 176

Query: 197 VMKRHGFKGMPASHGASLSHRSPGSTGQRDAPGKVFKGKKMPGRMGGKQRTVKNVWVYKI 256
            +KRH FK    +HG S SHR+ GS G    PG+V+KGKKMPGRMGG +  ++ + + K+
Sbjct: 177 GIKRHHFKRGQMTHG-SKSHRALGSIGAGTTPGRVYKGKKMPGRMGGTRTKIRKLKIVKV 236

Query: 257 DPARNLIWVKGQVPGAEGNFVFIKDA 281
           D   N++ +KG +PG  GN + I  A
Sbjct: 237 DKELNVVMIKGALPGKPGNLLRITPA 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LRN84.4e-13575.0850S ribosomal protein L3-2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RPL... [more]
Q2G8Y09.7e-5853.8850S ribosomal protein L3 OS=Novosphingobium aromaticivorans (strain ATCC 700278 ... [more]
Q5NQ643.7e-5749.1650S ribosomal protein L3 OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 ... [more]
Q5FTY32.4e-5652.5150S ribosomal protein L3 OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=rpl... [more]
Q5LLU62.6e-5552.5150S ribosomal protein L3 OS=Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / ... [more]
Match NameE-valueIdentityDescription
XP_022138212.13.27e-234100.0050S ribosomal protein L3-2, chloroplastic [Momordica charantia][more]
XP_022972230.14.82e-21491.7250S ribosomal protein L3-2, chloroplastic [Cucurbita maxima][more]
XP_022947107.17.92e-21189.9150S ribosomal protein L3-2, chloroplastic [Cucurbita moschata][more]
KAG7029037.12.27e-21089.6050S ribosomal protein L3-2, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
KAG6597593.14.58e-21089.9150S ribosomal protein L3-2, mitochondrial, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
A0A6J1CAF71.58e-234100.0050S ribosomal protein L3-2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1I7Y92.33e-21491.7250S ribosomal protein L3-2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1G5I53.84e-21189.9150S ribosomal protein L3-2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
E5GC402.50e-19884.7650S ribosomal protein l3 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3AYD12.50e-19884.7650S ribosomal protein L3-2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10348392... [more]
Match NameE-valueIdentityDescription
AT3G17465.13.1e-13675.08ribosomal protein L3 plastid [more]
AT2G43030.11.5e-3741.75Ribosomal protein L3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.160.810coord: 107..170
e-value: 7.7E-23
score: 82.7
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 200..284
e-value: 3.0E-27
score: 97.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..242
NoneNo IPR availablePANTHERPTHR11229:SF15BNAA01G27990D PROTEINcoord: 1..321
IPR019927Ribosomal protein L3, bacterial/organelle-typeTIGRFAMTIGR03625TIGR03625coord: 77..278
e-value: 2.7E-77
score: 257.1
IPR019927Ribosomal protein L3, bacterial/organelle-typePANTHERPTHR1122950S RIBOSOMAL PROTEIN L3coord: 1..321
IPR019927Ribosomal protein L3, bacterial/organelle-typeHAMAPMF_01325_BRibosomal_L3_Bcoord: 75..281
score: 34.444241
IPR000597Ribosomal protein L3PFAMPF00297Ribosomal_L3coord: 173..256
e-value: 4.6E-19
score: 68.6
IPR019926Ribosomal protein L3, conserved sitePROSITEPS00474RIBOSOMAL_L3coord: 175..198
IPR009000Translation protein, beta-barrel domain superfamilySUPERFAMILY50447Translation proteinscoord: 65..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0709.1MC05g0709.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006412 translation
cellular_component GO:0005762 mitochondrial large ribosomal subunit
cellular_component GO:0005840 ribosome
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003735 structural constituent of ribosome