MC05g0648 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0648
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pectin lyase-like superfamily protein
Location	MC05: 4968297 .. 4971998 (+)
RNA-Seq Expression	MC05g0648
Synteny	MC05g0648

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTTTCACCTCTTCTGATCTTCTTCTGTTTTCTTCTAGCCGTTTCCGTGTCTCTCAGTTGCGCCAGAGATTTTGAATCCGCCGCCGATGCATTGGATTTCCTTCGTTCGCCGGCGGACGCGGCGGAGCCTCCCGGAGGAGACCCATGGCGGATTCCACGTATTTTTGATGTTACTGATTATGGAGCAGTTGCTGATGGAAATACGGAGAGCAGTTTGGTAAGTCACTTGCTTTTATACACAAATTCCATATATATATATATATATATATATTATTTTATTCCATTATATTTTAATTTATTTTTAAAAGAAAAATATTTACGTGAAACTTATTTATATGGTTGAAAAATTGAAAGTTTATTATTAAAAAAAAAATCTATATTTTTTTATTTCTATATCTTATCCTTCGGGGGAATAAGAAAACTTTGGTGCCAATTATTTCTCTAACAATTGTGAATAGGGGTGTCATAATTGACAATATATACTTCAATTAATTGAGGATTCTCCCCCGATGACTTTTGAGAAGATATTCATTGAGCTAAAATTTGATTATATTCATGGTTCTTGGAAAAGGCAAACTGTTTTATTAGTCAAGTGTGTCATTTCTATACATTTCTATGTCCTTTGGAATAATTGTTTTCCTTGATAGATTGTGACGGTGATGTGTGGTTACCACTATTTAGATGTAACCTTTAATTGTATTATTTCTATTTAATGCAATATTTTGTGTTTCAAAAAAAATATTTAATAGTGAAAAGAACCATGTATTTTCTAAAATATTTTTAAAATTGAGAAAAAGAAAATTGAACCACTCTAAATTTAAATTTTATAGCATTAAAATCTAACCTTGAATAAATGTGACCTCAAAACTAATTTTACACTAAATTTAACTTGTAAAATATTTGTTGTAAAAAAAATGTAAAATATTTAAAATCAAACCACAAAAATTAGAAATCAACCTTTGCTAATCAATTTTGTTTTTGGTTTGTTATTTTTAAAATTAACCTTATAAACACTTTTTTTTCCTATCTATTTATTTGTTTCATTGTTTTTTATATAATTAAAAAAAAATTACAGGCTTGTTTTTGTTTTTTAAATTTGATTAAAAATTCATATTTGATAACCTTACTAATGCAAATCTAAAAAACAAGCTTAATTAAAAAAACAAAATGTATCAAATGATACTGAAAATATTTCGCCTGGCCGCAAAATCTAGCAAGCAAAAAATTACCCAGATCCACCAATGAAAATGTCCCTTAAAAACAAAAAGAACCAATTCCTTTCGTTTTATTTTTAATCTTATTTTCCTATCAAATTTGAACATGACATTACCATCAATATTGTTTTAAAATATAAATATTACTTCATACCGAAGAGTTTTCGTTAGGTGTAAAACCTGTATTAAAGCTTTAAAAAAAGAGACTAAAATGAATTAAAGTTAAATATAATAATCCAAATAAATATTATAAACTAAAATGAATCAATATTAAAATACTAGATCTATAAAATTTTGTTGAGGTATAGGAATTCATGGTAGCGTGGGAGGAGGCATGCGCTTATCCAGGAAGGTCGGTGTTGTACGTACCACAGGGCACATTCTTCGTCGGAGAAACTGTATTTTCCGGCCCCTGTTTCAACGACCTCTCTCCCAAAGTCCTCATTTTAGGAACCTTGAGAGCTCCGACCGCCCTCGACGACGCCGACGTCTGGATCCACTTCAAAAGCCTCCATCACCTCGACATCACTGGTGGCGACACCAATACCACCGCCATTCTCGACGGTCAGGGCGCTCAAACCTGGTCCAATTCTGCCTGCCGTCGCCACTTCACTTGCGACATATTTGCCACCGTAAGTATATTCAGAGACCATAATAAATCGATGTATACCTTAATTAGTTGAGTCAGGCTTTAATTTAATTTTCAATTTTATGTCTATAAATTTTTGGTACGTCAGATAAGTTCTTAAATATTCAATTTTGTTATATATTCTTATATTTTTTTAAAAAAATTTCACTAAATCAATTAGGTATTAAAATTAAACATTCTTTTATTTTAAAACGAGTTGGGGTAACTTATTAGATTCATAAGATAACTATGAGACATTTTAAAATTTAGGGATTTATTAGACACAAATTAAATTAAAAGTTTATCGACTATCAATAGACACTATTTAAAGTGTATAGACTTATTTTTAATAGTGCTAAATTAAAAATTTATTTACAAAATATACCTCTCCTCAAACTTATTCTAAGAATAATGTTCGACAAATTGTAAAGAAACTACCTATGTTACTCCATAGTTTTCAACTTGCAAAGTGCCTACAGTTATTCGAGCACTATTTCCCAAATAAGTTTCGGGAAAGGTATATTTTGCAAATAAGTTCCTAATTTAGCATTATTAAAAAAATCTGTGACTTATTAAAGGTGTAATTTTACCTTTGCTGTTAGTTGCATTGCAATTGCCCTCCCCTTCTATTTTTTGAATCAAAACACCAAAATCCAACCTTTTTCTCTCTCCCAATTTGGTGGATCAGTCTTTGAAGCTGAGCGATGTGTCATATGGAAGGGTGAGCAATATTGAGGTTGTGGACAGCAAAGGTTTCCATGTTGCTCTTCATGGTTGTGACAATATTCATCTCTCTGGATTCAATATTAGAGCTCCAGGGGACAGTCAGAACACCGACGGCATCCATGTTAGTCAGTCTACCAACATCAACATTACTTCCTCCGATATTGGCGTCGGCGACGATTGTGTTTCCATCGGACCCGGCAGCGTTAACGTGTCCGTTTCCGACATTAACTGTGGTCCGGGACATGGAATTAGGTATCTTTTATCTACCATCTACTTTCTACTTTCTAATTAGTGTTGTGTTTGTAGAAAAAAGTACGTGAAATGGTAGAATCTTGAACAAGTAGTGTTTTTGGGTATTTGGAATAATATAATGCAGTGTGGGGAGCCTAGGGAGGTACCAAAATGAGATGGATGTGGTGGGAATAAGAGTGGAGAATTGTTCCATAAATGGCACTACAAATGGAGTGAGGATAAAGACATGGCCAGGACAGCATTCAAGCATAGCTACCAATTTGACATTTCAAAACATTGTCATGTCCAATGTCTCAAACCCCATTGTCATTGACCAACAATATTGTCCCAACCATTCTTGCAAAACACATGAGGTAATTAACTACTCTTAATATGACCCTACTCCACATAAAACTCATTCTTAACCATTAACAATTAACAAAACGCTGGTGTGACATGTTCGTTTGATAAATTTTGGCTAAGTTAGTGTAATGTGTAACAAAACAGTGTTGGATAGATAACGATGATCATGTCACATTCAGTGTTATGATTATGGTATTAACTACAAGTAGTACTTTTGGTCTGACTCGAAGTTGTGTTTTGAGAATAGTTTTAGTTTAGAAAAATACTTTAATTAATATACTTTCATTAAAAGCACTTGTTTTTGAGGCAGGCATCGAATGTGAAGCTGAGTGAGATAAAGATGAGAAACATAAGTGGGAGTTACAACTCAGAGTTTGCAGTAAATTTGCAGTGCAGCTCAGGGAAGGCTTGTGAGAAAATTGAGCTTTCTTACATAAATCTCACTGCCACTCAGCCACCTGATCAAAACCACCAAATCGGGCGATTCAACTGCAACGGTGCCCTTCATGCCTTCACTATTCTTAATTCTACATTT

mRNA sequence

ATGAAGCTTTCACCTCTTCTGATCTTCTTCTGTTTTCTTCTAGCCGTTTCCGTGTCTCTCAGTTGCGCCAGAGATTTTGAATCCGCCGCCGATGCATTGGATTTCCTTCGTTCGCCGGCGGACGCGGCGGAGCCTCCCGGAGGAGACCCATGGCGGATTCCACGTATTTTTGATGTTACTGATTATGGAGCAGTTGCTGATGGAAATACGGAGAGCAGTTTGGAATTCATGGTAGCGTGGGAGGAGGCATGCGCTTATCCAGGAAGGTCGGTGTTGTACGTACCACAGGGCACATTCTTCGTCGGAGAAACTGTATTTTCCGGCCCCTGTTTCAACGACCTCTCTCCCAAAGTCCTCATTTTAGGAACCTTGAGAGCTCCGACCGCCCTCGACGACGCCGACGTCTGGATCCACTTCAAAAGCCTCCATCACCTCGACATCACTGGTGGCGACACCAATACCACCGCCATTCTCGACGGTCAGGGCGCTCAAACCTGGTCCAATTCTGCCTGCCGTCGCCACTTCACTTGCGACATATTTGCCACCTCTTTGAAGCTGAGCGATGTGTCATATGGAAGGGTGAGCAATATTGAGGTTGTGGACAGCAAAGGTTTCCATGTTGCTCTTCATGGTTGTGACAATATTCATCTCTCTGGATTCAATATTAGAGCTCCAGGGGACAGTCAGAACACCGACGGCATCCATGTTAGTCAGTCTACCAACATCAACATTACTTCCTCCGATATTGGCGTCGGCGACGATTGTGTTTCCATCGGACCCGGCAGCGTTAACGTGTCCGTTTCCGACATTAACTGTGGTCCGGGACATGGAATTAGTGTGGGGAGCCTAGGGAGGTACCAAAATGAGATGGATGTGGTGGGAATAAGAGTGGAGAATTGTTCCATAAATGGCACTACAAATGGAGTGAGGATAAAGACATGGCCAGGACAGCATTCAAGCATAGCTACCAATTTGACATTTCAAAACATTGTCATGTCCAATGTCTCAAACCCCATTGTCATTGACCAACAATATTGTCCCAACCATTCTTGCAAAACACATGAGGCATCGAATGTGAAGCTGAGTGAGATAAAGATGAGAAACATAAGTGGGAGTTACAACTCAGAGTTTGCAGTAAATTTGCAGTGCAGCTCAGGGAAGGCTTGTGAGAAAATTGAGCTTTCTTACATAAATCTCACTGCCACTCAGCCACCTGATCAAAACCACCAAATCGGGCGATTCAACTGCAACGGTGCCCTTCATGCCTTCACTATTCTTAATTCTACATTT

Coding sequence (CDS)

ATGAAGCTTTCACCTCTTCTGATCTTCTTCTGTTTTCTTCTAGCCGTTTCCGTGTCTCTCAGTTGCGCCAGAGATTTTGAATCCGCCGCCGATGCATTGGATTTCCTTCGTTCGCCGGCGGACGCGGCGGAGCCTCCCGGAGGAGACCCATGGCGGATTCCACGTATTTTTGATGTTACTGATTATGGAGCAGTTGCTGATGGAAATACGGAGAGCAGTTTGGAATTCATGGTAGCGTGGGAGGAGGCATGCGCTTATCCAGGAAGGTCGGTGTTGTACGTACCACAGGGCACATTCTTCGTCGGAGAAACTGTATTTTCCGGCCCCTGTTTCAACGACCTCTCTCCCAAAGTCCTCATTTTAGGAACCTTGAGAGCTCCGACCGCCCTCGACGACGCCGACGTCTGGATCCACTTCAAAAGCCTCCATCACCTCGACATCACTGGTGGCGACACCAATACCACCGCCATTCTCGACGGTCAGGGCGCTCAAACCTGGTCCAATTCTGCCTGCCGTCGCCACTTCACTTGCGACATATTTGCCACCTCTTTGAAGCTGAGCGATGTGTCATATGGAAGGGTGAGCAATATTGAGGTTGTGGACAGCAAAGGTTTCCATGTTGCTCTTCATGGTTGTGACAATATTCATCTCTCTGGATTCAATATTAGAGCTCCAGGGGACAGTCAGAACACCGACGGCATCCATGTTAGTCAGTCTACCAACATCAACATTACTTCCTCCGATATTGGCGTCGGCGACGATTGTGTTTCCATCGGACCCGGCAGCGTTAACGTGTCCGTTTCCGACATTAACTGTGGTCCGGGACATGGAATTAGTGTGGGGAGCCTAGGGAGGTACCAAAATGAGATGGATGTGGTGGGAATAAGAGTGGAGAATTGTTCCATAAATGGCACTACAAATGGAGTGAGGATAAAGACATGGCCAGGACAGCATTCAAGCATAGCTACCAATTTGACATTTCAAAACATTGTCATGTCCAATGTCTCAAACCCCATTGTCATTGACCAACAATATTGTCCCAACCATTCTTGCAAAACACATGAGGCATCGAATGTGAAGCTGAGTGAGATAAAGATGAGAAACATAAGTGGGAGTTACAACTCAGAGTTTGCAGTAAATTTGCAGTGCAGCTCAGGGAAGGCTTGTGAGAAAATTGAGCTTTCTTACATAAATCTCACTGCCACTCAGCCACCTGATCAAAACCACCAAATCGGGCGATTCAACTGCAACGGTGCCCTTCATGCCTTCACTATTCTTAATTCTACATTT

Protein sequence

MKLSPLLIFFCFLLAVSVSLSCARDFESAADALDFLRSPADAAEPPGGDPWRIPRIFDVTDYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRRHFTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWPGQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF

Homology

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BLAST of MC05g0648 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.2e-76
Identity = 143/347 (41.21%), Postives = 208/347 (59.94%), Query Frame = 0


Query: 56  IFDVTDYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLS 115
           +F+V DYGA   G+   ++  M AW+ ACA  G S + +P+G + +GE    GPC     
Sbjct: 9   VFNVNDYGAKGAGDISQAV--MKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPC-KGSK 68

Query: 116 PKVLILGTLRAPT--ALDDADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRR 175
               I G ++AP   +   +D W+ F  +  L ++G     T  LDGQG   W+ + C +
Sbjct: 69  IGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSG-----TGTLDGQGQTAWAKNNCDK 128

Query: 176 HFTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDG 235
           +  C   A +L+   + +  V +I  ++SK FH+ +  C++I      + APG S NTDG
Sbjct: 129 NPNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDG 188

Query: 236 IHVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVV 295
           IHV  S  + IT++ I  GDDC+SIGPGS NV+++ +NCGPGHGIS+GSLGRY NE +V 
Sbjct: 189 IHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVR 248

Query: 296 GIRVENCSINGTTNGVRIKTWPGQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKT 355
           GI V+ C+ +GT NGVR+KTWP      AT+LTFQ++ M+NV NP+++DQ+YCP   C  
Sbjct: 249 GITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSR 308

Query: 356 HEASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINLT 401
              S +KLS I   NI G+   + AV + CS G  C  +++  INL+
Sbjct: 309 QAPSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLS 347

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BLAST of MC05g0648 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.8e-76
Identity = 145/350 (41.43%), Postives = 213/350 (60.86%), Query Frame = 0


Query: 54  PRIFD-VTDYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFN 113
           P  FD V  +GA ADG T+ S  F+ AW+EACA    S + +P+GT+ + +    GPC  
Sbjct: 26  PDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKA 85

Query: 114 DLSPKVLILGTLRA---PTALDDADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNS 173
            +  ++ + GT++A   P+A  D + W+ F S+ +  + GG      I DGQG+  +  +
Sbjct: 86  PI--EINVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGG-----GIFDGQGSIAYEKN 145

Query: 174 ACRRHFTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQ 233
            C           +++   V+   + +I   DSK FH+ +  C NI L    I AP +S 
Sbjct: 146 TCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESP 205

Query: 234 NTDGIHVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNE 293
           NTDGIH+ +S  +NI +SDI  GDDC+SIG G+ N+ + +I CGPGHGIS+GSLG++QNE
Sbjct: 206 NTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNE 265

Query: 294 MDVVGIRVENCSINGTTNGVRIKTWPGQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNH 353
             V GI++ NC+I  T+NG RIKTWPG+H    + + F++I M+NVS+PI+IDQQYCP +
Sbjct: 266 EPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWN 325

Query: 354 SCKTHEASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINL 400
            CK +E S VKLS I  +NI G+     A+   CS    C+ +EL+ I++
Sbjct: 326 KCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDI 367

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BLAST of MC05g0648 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.8e-75
Identity = 141/344 (40.99%), Postives = 210/344 (61.05%), Query Frame = 0


Query: 59  VTDYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKV 118
           V  +GA ADG T+ S  F+ AW+EACA    S + +P+GT+ + +    GPC   +  ++
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPI--EI 91

Query: 119 LILGTLRA---PTALDDADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRRHF 178
            + GT++A   P+A  D + W+ F S+ +  + GG      I DGQG+  +  + C    
Sbjct: 92  NVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGG-----GIFDGQGSIAYEKNTCENRE 151

Query: 179 TCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIH 238
                  +++   ++   + +I   DSK FH+ +  C NI L    I AP +S NTDGIH
Sbjct: 152 FRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIH 211

Query: 239 VSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGI 298
           + +S  +NI +SDI  GDDC+SIG G+ N+ + +I CGPGHGIS+GSLG++QNE  V GI
Sbjct: 212 MGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGI 271

Query: 299 RVENCSINGTTNGVRIKTWPGQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHE 358
           ++ NC+I  T+NG RIKTWPG+H    + + F++I M+NVS+PI+IDQQYCP + CK +E
Sbjct: 272 KISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNE 331

Query: 359 ASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINL 400
            S VKLS I  +NI G+     A+   CS    C+ +EL+ I++
Sbjct: 332 ESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDI 367

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BLAST of MC05g0648 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.8e-73
Identity = 143/361 (39.61%), Postives = 216/361 (59.83%), Query Frame = 0


Query: 43  AEPPGGDPWRIPRIFDVTDYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVG 102
           A  PGG        FD+T  GA  +G T+S+     AW  AC   G+  + +P+G F VG
Sbjct: 34  ASGPGGS-------FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVG 93

Query: 103 ETVFSGPCFNDLSPKVLILGTLRAPTALD---DADVWIHFKSLHHLDITGGDTNTTAILD 162
              F+GPC  D++  + + G L A T L    D   WI    + +L ITG        LD
Sbjct: 94  PLNFTGPCKGDVT--IQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITG-----KGKLD 153

Query: 163 GQGAQTWSNSACRRHFTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSG 222
           GQG   WS ++C + + C I   SL +  V+ G VS I +++SK FH+ ++ C ++ +  
Sbjct: 154 GQGPAVWSKNSCVKKYDCKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKD 213

Query: 223 FNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGIS 282
            N+ APGDS NTDGIH+  S+ + IT++ IGVGDDC+SIGPG+  V+++ + CGPGHGIS
Sbjct: 214 VNVTAPGDSPNTDGIHMGDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGIS 273

Query: 283 VGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWPGQHSSI-ATNLTFQNIVMSNVSNP 342
           +GSLGRY++E DV  I V++C++  T NGVRIK +    S + A+ + ++NI M +   P
Sbjct: 274 IGSLGRYKDEKDVTDINVKDCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYP 333

Query: 343 IVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYIN 400
           I+ID +YCPN  C  + AS V + ++  +NI+G+ ++  AVNL C++   C  + +  +N
Sbjct: 334 IIIDMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVN 380

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BLAST of MC05g0648 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 275.8 bits (704), Expect = 8.4e-73
Identity = 143/345 (41.45%), Postives = 201/345 (58.26%), Query Frame = 0


Query: 58  DVTDYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPK 117
           DV   GA  D  T+ S  F  AW+EACA    S + VP+G + V    F GPC   ++  
Sbjct: 71  DVKASGAKGDSKTDDSAAFAAAWKEACA--AGSTITVPKGEYMVESLEFKGPCKGPVT-- 130

Query: 118 VLILGTLRAPTALDDADV---WIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRRH 177
           + + G  +AP  +        WI F+++    + G      AI DGQG+  W  + C + 
Sbjct: 131 LELNGNFKAPATVKTTKPHAGWIDFENIADFTLNG----NKAIFDGQGSLAWKANDCAKT 190

Query: 178 FTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGI 237
             C+    +++ + ++  ++++I   +SK FH+ +  C NI LS   I AP +S NTDGI
Sbjct: 191 GKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGI 250

Query: 238 HVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVG 297
           H+ +S  +N+  + I  GDDCVSIG G+ N+ V ++ CGPGHGIS+GSLGRY NE  V G
Sbjct: 251 HIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKG 310

Query: 298 IRVENCSINGTTNGVRIKTWPGQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTH 357
           + V  C I  T NGVRIKTWPG    IA+N+ F++I M NVS P++IDQ+YCP   CK  
Sbjct: 311 VTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAG 370

Query: 358 EASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINL 400
             S VKLS++ ++ I G+  ++ AV L CS G  C  I LS INL
Sbjct: 371 VPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINL 407

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BLAST of MC05g0648 vs. NCBI nr
Match: XP_022138060.1 (exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 727 bits (1876), Expect = 2.15e-263
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0


Query: 77  MVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVW 136
           MVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVW
Sbjct: 1   MVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVW 60

Query: 137 IHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRRHFTCDIFATSLKLSDVSYGRVSN 196
           IHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRRHFTCDIFATSLKLSDVSYGRVSN
Sbjct: 61  IHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRRHFTCDIFATSLKLSDVSYGRVSN 120

Query: 197 IEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDCV 256
           IEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDCV
Sbjct: 121 IEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDCV 180

Query: 257 SIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWPG 316
           SIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWPG
Sbjct: 181 SIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWPG 240

Query: 317 QHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYNSE 376
           QHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYNSE
Sbjct: 241 QHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYNSE 300

Query: 377 FAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF 430
           FAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF
Sbjct: 301 FAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF 354

[Close]

BLAST of MC05g0648 vs. NCBI nr
Match: XP_038885913.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 595 bits (1535), Expect = 2.15e-210
Identity = 301/431 (69.84%), Postives = 354/431 (82.13%), Query Frame = 0


Query: 1   MKLSPLLIFFCFLLAVSVSLSCARDFESAADALDFLRSPADAAEPPGGDPWRIPRIFDVT 60
           MKLS  L+ F FLLA+S+S   AR+F SAA + DFL  P+ +AE P GDPW   R+FDV 
Sbjct: 1   MKLS--LLVFSFLLALSLS---ARNF-SAAGSFDFLLPPSYSAESPAGDPWMGLRVFDVN 60

Query: 61  DYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLI 120
           DYGA+ADG TE+SL FM AWE+AC Y G S++ +P+G FFV +  FSGPCFND+SPKVLI
Sbjct: 61  DYGAIADGKTENSLAFMSAWEDACGYNGSSMVLIPEGNFFVDQVTFSGPCFNDISPKVLI 120

Query: 121 LGTLRAPTALDDADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSN-SACRRHFTCDI 180
           LGTL APT+L D D WI F+SL    +TGG+++  AILDGQGA TWS+ SACR   TCD 
Sbjct: 121 LGTLIAPTSLAD-DFWIRFQSLRRFILTGGESS--AILDGQGAPTWSSGSACRHRMTCDA 180

Query: 181 FATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQS 240
           FATSLKLS++S G V+NI +VDSKGFHV+++GCDNIHLSGFNI AP DSQNTDGIH+SQS
Sbjct: 181 FATSLKLSNISNGIVNNIRLVDSKGFHVSIYGCDNIHLSGFNITAPWDSQNTDGIHISQS 240

Query: 241 TNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVEN 300
           TNI+IT SDIGVGDDCVS+GPG VNVS+SDI CGPGHGISVGSLG+Y+NE DV+GIRVEN
Sbjct: 241 TNISITISDIGVGDDCVSLGPGCVNVSISDIRCGPGHGISVGSLGKYKNENDVMGIRVEN 300

Query: 301 CSINGTTNGVRIKTWPGQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNV 360
           C+INGT NGVR+KTWPG   S ATNLTFQNIVM NVSNPI+IDQ YCPNHSC   E+S+V
Sbjct: 301 CTINGTQNGVRVKTWPGDLVSNATNLTFQNIVMINVSNPIIIDQHYCPNHSCDPQESSSV 360

Query: 361 KLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGA 420
           KLS+I +RNISG+YN+EFAVN+QCS+ K CE  +LSYINLTA QPP Q H+IG FNC GA
Sbjct: 361 KLSDINIRNISGTYNTEFAVNIQCSTTKPCENFQLSYINLTAIQPP-QTHKIGHFNCKGA 420

Query: 421 LHAFTILNSTF 430
           L++FTI NSTF
Sbjct: 421 LNSFTIFNSTF 421

[Close]

BLAST of MC05g0648 vs. NCBI nr
Match: XP_023539060.1 (exopolygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587 bits (1512), Expect = 9.36e-207
Identity = 304/438 (69.41%), Postives = 350/438 (79.91%), Query Frame = 0


Query: 1   MKLSPLLIFFCFLLAVSVSLSCARDF------ESAADALDFLRSPADAAEPPGGDPWRIP 60
           MKLS L+IF+ FLLAVSVS    R+F      ESAA + DFL  P D AEPP GDPWR  
Sbjct: 1   MKLSALIIFY-FLLAVSVS---GRNFSPEPALESAAGSWDFLLLPPDMAEPPVGDPWRGL 60

Query: 61  RIFDVTDYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDL 120
            +FDVTDYGA+ADG TE+SL FM AWE+ACAY G S L +P+G+FF+ + +FSGPCF DL
Sbjct: 61  AVFDVTDYGAIADGKTENSLAFMGAWEDACAYRGSSTLLIPEGSFFLAQVLFSGPCFGDL 120

Query: 121 SPKVLILGTLRAPTALDDADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSA-CRR 180
           SPKV+ILGTL APT L  ADVWI+FKSL HL ITG   +   ILDGQGA+TW     CR 
Sbjct: 121 SPKVIILGTLIAPTTLT-ADVWIYFKSLRHLTITGNVGS--GILDGQGAETWLYGFNCRH 180

Query: 181 HFTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDG 240
             +C IF TSLKLSDVS   VSNI +V+SKGFHVAL+GCDNIHLSGFNI AP +SQNTDG
Sbjct: 181 RLSCGIFTTSLKLSDVSNATVSNISLVNSKGFHVALNGCDNIHLSGFNITAPWNSQNTDG 240

Query: 241 IHVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVV 300
           IH+SQSTNINIT+SDI VGDDCVSIGPGS+NVSVS++ CGPGHGISVGSLGRY+ E DV+
Sbjct: 241 IHISQSTNINITTSDISVGDDCVSIGPGSINVSVSNVRCGPGHGISVGSLGRYREEKDVM 300

Query: 301 GIRVENCSINGTTNGVRIKTWPG-QHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCK 360
           GIRVENC+I  T NGVR+KTWPG  H+S ATNLTFQNIVM NVSNPI+IDQQYCPNH C 
Sbjct: 301 GIRVENCTIKDTQNGVRVKTWPGGDHASKATNLTFQNIVMFNVSNPIIIDQQYCPNHFCD 360

Query: 361 THEASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIG 420
             E+S VKLS I ++NISG+YNSEFAVNLQCSS   CE ++LSYINLTA +PP+ +  IG
Sbjct: 361 IKESSGVKLSNINIKNISGTYNSEFAVNLQCSSAMPCENVQLSYINLTAIEPPETD-DIG 420

Query: 421 RFNCNGALHAFTILNSTF 430
           RFNC G+L+ F+ILNSTF
Sbjct: 421 RFNCKGSLNNFSILNSTF 430

[Close]

BLAST of MC05g0648 vs. NCBI nr
Match: KAG7028995.1 (Exopolygalacturonase-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 585 bits (1509), Expect = 2.68e-206
Identity = 303/438 (69.18%), Postives = 350/438 (79.91%), Query Frame = 0


Query: 1   MKLSPLLIFFCFLLAVSVSLSCARDF------ESAADALDFLRSPADAAEPPGGDPWRIP 60
           MKLS L++F+ FLLAVSVS    R+F      ESAA + DFL  P D AEPP GDPWR  
Sbjct: 1   MKLSSLVMFY-FLLAVSVS---GRNFSPEPALESAAGSWDFLLLPPDMAEPPVGDPWRGL 60

Query: 61  RIFDVTDYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDL 120
            +FDVTDYGA+ADG TE+SL FM AWE+ACAY G S L +P+G+FF+ + +FSGPCF DL
Sbjct: 61  AVFDVTDYGAIADGKTENSLAFMGAWEDACAYRGSSTLLIPEGSFFLAQVLFSGPCFGDL 120

Query: 121 SPKVLILGTLRAPTALDDADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSA-CRR 180
           SPKV+ILGTL APT L  ADVWI+FKSL HL ITG   +   ILDGQGA+TW     CR 
Sbjct: 121 SPKVIILGTLIAPTTLT-ADVWIYFKSLRHLTITGNVGS--GILDGQGAETWLYGFNCRH 180

Query: 181 HFTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDG 240
             +C IF TSLKLSDVS   VSNI +V+SKGFHVAL+GCDNIHLSGFNI AP +SQNTDG
Sbjct: 181 RLSCGIFTTSLKLSDVSNATVSNISLVNSKGFHVALNGCDNIHLSGFNITAPWNSQNTDG 240

Query: 241 IHVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVV 300
           IH+SQSTNINIT+SDI VGDDCVSIGPGS+NVSVS++ CGPGHGISVGSLGRY+ E DV+
Sbjct: 241 IHISQSTNINITASDISVGDDCVSIGPGSINVSVSNVRCGPGHGISVGSLGRYREEKDVI 300

Query: 301 GIRVENCSINGTTNGVRIKTWPG-QHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCK 360
           GIRVENC+I  T NGVR+KTWPG  H+S ATNLTFQNIVM NVSNPI+IDQQYCPNH C 
Sbjct: 301 GIRVENCTIKDTQNGVRVKTWPGGDHASKATNLTFQNIVMFNVSNPIIIDQQYCPNHLCD 360

Query: 361 THEASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIG 420
             E+S VKLS I ++NISG+YNSEFAVNLQCSS   CE ++LSYINLTA +PP+ +  IG
Sbjct: 361 IKESSGVKLSNINIKNISGTYNSEFAVNLQCSSAVPCENVQLSYINLTAIEPPETD-DIG 420

Query: 421 RFNCNGALHAFTILNSTF 430
           RFNC G+L+ F+ILNSTF
Sbjct: 421 RFNCKGSLNNFSILNSTF 430

[Close]

BLAST of MC05g0648 vs. NCBI nr
Match: XP_004134495.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 536 bits (1381), Expect = 6.38e-187
Identity = 278/438 (63.47%), Postives = 328/438 (74.89%), Query Frame = 0


Query: 1   MKLSPLLIFFCFLLAVSVSLSCARDFESAADALDFLRSPADAAEPPGGDPWRIPRIFDVT 60
           MKL   L  F  LLA+++S         AAD  D    P+  AEPP GDPW   R+FDV 
Sbjct: 1   MKLISSLSLFSSLLAITLS---------AADGFDIALPPSYPAEPPVGDPWSGLRVFDVN 60

Query: 61  DYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLI 120
           DYG VADG T +SL  M AWE+AC + G S+L +P+G +FV +  FSGPCFNDLSPKVLI
Sbjct: 61  DYGGVADGKTNNSLALMAAWEDACYHQGGSMLLIPEGNYFVDQVTFSGPCFNDLSPKVLI 120

Query: 121 LGTLRAPTALDDADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSN-SACRRHFTCDI 180
           LGTL APT L  ADVWIHF SL  L +T G  +T   LDG+GAQTWS+ S CR   TC +
Sbjct: 121 LGTLIAPTNLT-ADVWIHFDSLRRLSLTAGHLST---LDGRGAQTWSSGSRCRHAMTCAM 180

Query: 181 FATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQS 240
           FA SLKLS+V  G VS+I +VDSK FHV++H CDNIHLSGFNI AP  SQNTDG H+SQS
Sbjct: 181 FAISLKLSNVLNGTVSSINLVDSKAFHVSIHQCDNIHLSGFNITAPWYSQNTDGFHISQS 240

Query: 241 TNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVEN 300
           TNI+IT+S IGVGDDCVSIGPGSVNV VS++ CGPGHGIS+GSLGRY+ E DV GI VEN
Sbjct: 241 TNISITNSKIGVGDDCVSIGPGSVNVLVSNVTCGPGHGISIGSLGRYKMENDVRGIWVEN 300

Query: 301 CSINGTTNGVRIKTWPGQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNV 360
           C+I+GT NGVR+KTWPG H+S ATNLTFQNIVM NVSNPI+IDQQYCPN SC + E+S+V
Sbjct: 301 CTISGTQNGVRVKTWPGDHASNATNLTFQNIVMINVSNPIIIDQQYCPNSSCDSRESSSV 360

Query: 361 KLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINLTAT-------QPPDQNHQIG 420
           KLS+I +RNISG+YNSEFA+N++CSS KACE I LSYINLT+        +  ++    G
Sbjct: 361 KLSDINIRNISGTYNSEFAINMKCSSTKACENIRLSYINLTSINHQSDDDEDEEEGDVTG 420

Query: 421 RFNCNGALHAFTILNSTF 430
            FN  GAL++FTI NSTF
Sbjct: 421 NFNFKGALNSFTIFNSTF 425

[Close]

BLAST of MC05g0648 vs. ExPASy TrEMBL
Match: A0A6J1C8D5 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111009318 PE=3 SV=1)

HSP 1 Score: 727 bits (1876), Expect = 1.04e-263
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0


Query: 77  MVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVW 136
           MVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVW
Sbjct: 1   MVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVW 60

Query: 137 IHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRRHFTCDIFATSLKLSDVSYGRVSN 196
           IHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRRHFTCDIFATSLKLSDVSYGRVSN
Sbjct: 61  IHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRRHFTCDIFATSLKLSDVSYGRVSN 120

Query: 197 IEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDCV 256
           IEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDCV
Sbjct: 121 IEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDCV 180

Query: 257 SIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWPG 316
           SIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWPG
Sbjct: 181 SIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWPG 240

Query: 317 QHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYNSE 376
           QHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYNSE
Sbjct: 241 QHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYNSE 300

Query: 377 FAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF 430
           FAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF
Sbjct: 301 FAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF 354

[Close]

BLAST of MC05g0648 vs. ExPASy TrEMBL
Match: A0A0A0L932 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G164560 PE=3 SV=1)

HSP 1 Score: 536 bits (1381), Expect = 3.09e-187
Identity = 278/438 (63.47%), Postives = 328/438 (74.89%), Query Frame = 0


Query: 1   MKLSPLLIFFCFLLAVSVSLSCARDFESAADALDFLRSPADAAEPPGGDPWRIPRIFDVT 60
           MKL   L  F  LLA+++S         AAD  D    P+  AEPP GDPW   R+FDV 
Sbjct: 1   MKLISSLSLFSSLLAITLS---------AADGFDIALPPSYPAEPPVGDPWSGLRVFDVN 60

Query: 61  DYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLI 120
           DYG VADG T +SL  M AWE+AC + G S+L +P+G +FV +  FSGPCFNDLSPKVLI
Sbjct: 61  DYGGVADGKTNNSLALMAAWEDACYHQGGSMLLIPEGNYFVDQVTFSGPCFNDLSPKVLI 120

Query: 121 LGTLRAPTALDDADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSN-SACRRHFTCDI 180
           LGTL APT L  ADVWIHF SL  L +T G  +T   LDG+GAQTWS+ S CR   TC +
Sbjct: 121 LGTLIAPTNLT-ADVWIHFDSLRRLSLTAGHLST---LDGRGAQTWSSGSRCRHAMTCAM 180

Query: 181 FATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQS 240
           FA SLKLS+V  G VS+I +VDSK FHV++H CDNIHLSGFNI AP  SQNTDG H+SQS
Sbjct: 181 FAISLKLSNVLNGTVSSINLVDSKAFHVSIHQCDNIHLSGFNITAPWYSQNTDGFHISQS 240

Query: 241 TNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVEN 300
           TNI+IT+S IGVGDDCVSIGPGSVNV VS++ CGPGHGIS+GSLGRY+ E DV GI VEN
Sbjct: 241 TNISITNSKIGVGDDCVSIGPGSVNVLVSNVTCGPGHGISIGSLGRYKMENDVRGIWVEN 300

Query: 301 CSINGTTNGVRIKTWPGQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNV 360
           C+I+GT NGVR+KTWPG H+S ATNLTFQNIVM NVSNPI+IDQQYCPN SC + E+S+V
Sbjct: 301 CTISGTQNGVRVKTWPGDHASNATNLTFQNIVMINVSNPIIIDQQYCPNSSCDSRESSSV 360

Query: 361 KLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINLTAT-------QPPDQNHQIG 420
           KLS+I +RNISG+YNSEFA+N++CSS KACE I LSYINLT+        +  ++    G
Sbjct: 361 KLSDINIRNISGTYNSEFAINMKCSSTKACENIRLSYINLTSINHQSDDDEDEEEGDVTG 420

Query: 421 RFNCNGALHAFTILNSTF 430
            FN  GAL++FTI NSTF
Sbjct: 421 NFNFKGALNSFTIFNSTF 425

[Close]

BLAST of MC05g0648 vs. ExPASy TrEMBL
Match: A0A6J1FMM1 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111446788 PE=3 SV=1)

HSP 1 Score: 506 bits (1303), Expect = 1.61e-176
Identity = 253/356 (71.07%), Postives = 292/356 (82.02%), Query Frame = 0


Query: 77  MVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVW 136
           M AWE+ACAY G S L +P+G+FF+ + +FSGPCF DLSPKV+ILGTL APT L  ADVW
Sbjct: 1   MGAWEDACAYRGSSTLLIPEGSFFLAQVLFSGPCFGDLSPKVIILGTLIAPTTLT-ADVW 60

Query: 137 IHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSA-CRRHFTCDIFATSLKLSDVSYGRVS 196
           I+FKSL HL ITG   +   ILDGQGA+TW     CR   +C IF TSLKLSDVS   VS
Sbjct: 61  IYFKSLRHLTITGNVGS--GILDGQGAETWLYGFNCRHRLSCGIFTTSLKLSDVSNATVS 120

Query: 197 NIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDC 256
           NI +V+SKGFHVAL+GCDNIHLSGFNI AP +SQNTDGIH+SQSTNINIT+SDI VGDDC
Sbjct: 121 NISLVNSKGFHVALNGCDNIHLSGFNITAPWNSQNTDGIHISQSTNINITASDISVGDDC 180

Query: 257 VSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWP 316
           VSIGPGS+NVSVS++ CGPGHGISVGSLGRY+ E DV+GIRVENC+I  T NGVR+KTWP
Sbjct: 181 VSIGPGSINVSVSNVRCGPGHGISVGSLGRYREEKDVIGIRVENCTIKDTQNGVRVKTWP 240

Query: 317 G-QHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYN 376
           G  H+S ATNLTFQNIVM NVSNPI+IDQQYCPNH C   E+S VKLS I ++NISG+YN
Sbjct: 241 GGDHASKATNLTFQNIVMFNVSNPIIIDQQYCPNHLCDIKESSGVKLSNINIKNISGTYN 300

Query: 377 SEFAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF 430
           SEFAVNLQCSS   CE ++LSYINLTA +PP+ +  IGRFNC G+L+ F+ILNSTF
Sbjct: 301 SEFAVNLQCSSAVPCENVQLSYINLTAIEPPETD-DIGRFNCKGSLNNFSILNSTF 352

[Close]

BLAST of MC05g0648 vs. ExPASy TrEMBL
Match: A0A6J1GXB0 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111457998 PE=3 SV=1)

HSP 1 Score: 504 bits (1297), Expect = 1.27e-175
Identity = 247/355 (69.58%), Postives = 292/355 (82.25%), Query Frame = 0


Query: 77  MVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVW 136
           M AWEEACA+ G S+  +P+GTF V    FSGPCFN+LSPKVLI  TL APT L   DVW
Sbjct: 1   MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLT-TDVW 60

Query: 137 IHFKSLHHLDITGGDTNTTAILDGQGAQTWS-NSACRRHFTCDIFATSLKLSDVSYGRVS 196
           IHF SL HL +TG D +  A+L+GQGAQTWS  SACRR  TC IF TSLKL+DVS+  V 
Sbjct: 61  IHFHSLRHLLLTGLDGS--AVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVK 120

Query: 197 NIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDC 256
           +I +VDSKGFHVA+HGCD+IHL GFNI AP +SQNTDGIH+S STNIN+ +S IGVGDDC
Sbjct: 121 DISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDC 180

Query: 257 VSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWP 316
           VSIGPGS +V VS+I CGPGHGISVGSLGRY+NEMDV+GIRVENC+I+ T NGVR+KTWP
Sbjct: 181 VSIGPGSFDVLVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWP 240

Query: 317 GQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYNS 376
           G +SS A +LTFQNIVM+NVSNPI+IDQ+YCPNHSC   E+S+VKLSE+ MRNISG+YN+
Sbjct: 241 GNYSSNAAHLTFQNIVMNNVSNPIIIDQRYCPNHSCDMKESSSVKLSEMNMRNISGTYNT 300

Query: 377 EFAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF 430
           +FAVN+ CSS   C  + LSYINLTAT PP++ ++IGRFNC GAL+ FT+LNSTF
Sbjct: 301 KFAVNIDCSSIMPCNNVHLSYINLTATHPPEK-YEIGRFNCMGALNTFTVLNSTF 351

[Close]

BLAST of MC05g0648 vs. ExPASy TrEMBL
Match: A0A6J1I855 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470865 PE=3 SV=1)

HSP 1 Score: 498 bits (1283), Expect = 1.76e-173
Identity = 250/356 (70.22%), Postives = 290/356 (81.46%), Query Frame = 0


Query: 77  MVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPKVLILGTLRAPTALDDADVW 136
           M AWE+ACAY G S   +P+G+FF+ + +FSGPCF DLSPKV+ILGTL APT L  ADVW
Sbjct: 1   MGAWEDACAYRGSSTFLIPEGSFFLAQVLFSGPCFGDLSPKVIILGTLIAPTTLT-ADVW 60

Query: 137 IHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSA-CRRHFTCDIFATSLKLSDVSYGRVS 196
           I+FKSL  L ITG   +   ILDGQGA+TW     CR   +C IF TSLKLSDVS   VS
Sbjct: 61  IYFKSLRQLTITGNIGS--GILDGQGAETWLYGFNCRHRLSCGIFTTSLKLSDVSNATVS 120

Query: 197 NIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGIHVSQSTNINITSSDIGVGDDC 256
           NI +V+SKGFHVAL+GCDNIHLSGF I AP +SQNTDGIH+SQSTNINIT+SDI VGDDC
Sbjct: 121 NISLVNSKGFHVALNGCDNIHLSGFKITAPWNSQNTDGIHISQSTNINITASDISVGDDC 180

Query: 257 VSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVGIRVENCSINGTTNGVRIKTWP 316
           VSIGPGS+NVSVS++ CGPGHGISVGSLGRY+ E DV+GIRVENC+I  T NGVR+KTWP
Sbjct: 181 VSIGPGSINVSVSNVICGPGHGISVGSLGRYREEKDVMGIRVENCTIKDTQNGVRVKTWP 240

Query: 317 GQ-HSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTHEASNVKLSEIKMRNISGSYN 376
           G+ H+S ATNLTFQNIVM NVSNPI+IDQQYCPNH C   E+S VKLS I ++NISG+YN
Sbjct: 241 GEDHASKATNLTFQNIVMFNVSNPIIIDQQYCPNHFCDIKESSGVKLSNINIKNISGTYN 300

Query: 377 SEFAVNLQCSSGKACEKIELSYINLTATQPPDQNHQIGRFNCNGALHAFTILNSTF 430
           SEFAVNLQCSS   CE ++LSYINLTA +PP+ +  IGRFNC G+L+ F+ILNSTF
Sbjct: 301 SEFAVNLQCSSAMPCENVQLSYINLTAIEPPETD-DIGRFNCKGSLNNFSILNSTF 352

[Close]

BLAST of MC05g0648 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 293.1 bits (749), Expect = 3.6e-79
Identity = 159/349 (45.56%), Postives = 215/349 (61.60%), Query Frame = 0


Query: 56  IFDVTDYGAVADGNTESSLEFMVAWEEACAYPGRSV-LYVPQGTFFVGETVFSGPCFNDL 115
           + DV  +GA A+ + + +  F+ AW++AC     SV L +P+G F VG   FSGPC N  
Sbjct: 34  LVDVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGPCTNVS 93

Query: 116 SPKVLILGTLRAPTALD---DADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSAC 175
           +  V +    +A T L        WI F  ++ L +TGG T      DGQGA  W  + C
Sbjct: 94  NLTVRV----KASTDLSKYRSGGGWIQFGWINGLTLTGGGT-----FDGQGALAWPFNNC 153

Query: 176 RRHFTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNT 235
                C +  TSLK   ++   V  I  V+SK FH+AL  C +   +  NI AP DS NT
Sbjct: 154 TSDSNCKLLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNT 213

Query: 236 DGIHVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMD 295
           DGIH+ +S+N+  + S I  GDDCVSIG G+  ++++ I CGPGHGISVGSLGRY NE D
Sbjct: 214 DGIHIERSSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKD 273

Query: 296 VVGIRVENCSINGTTNGVRIKTWPGQHS-SIATNLTFQNIVMSNVSNPIVIDQQYCPNHS 355
           V G+ V++C I+GTTNG+RIKTW      S ATN+TF+NI+M+NV+NPI+IDQ YCP  S
Sbjct: 274 VNGLVVKDCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSS 333

Query: 356 CKTHEASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINL 400
           C ++  S V+LSEI  +NI G+ +S  AV L CS G  C+K+ L  ++L
Sbjct: 334 CISNVPSKVELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHL 373

[Close]

BLAST of MC05g0648 vs. TAIR 10
Match: AT2G33160.1 (glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein )

HSP 1 Score: 292.7 bits (748), Expect = 4.7e-79
Identity = 155/367 (42.23%), Postives = 230/367 (62.67%), Query Frame = 0


Query: 54  PRIFDVTDYGAVADGNTESSLEFMVAWEEACAYP-GRSVLYVPQGTFFVGETVFSGPCFN 113
           P+IFDV +YGA AD   +++  F  AW EAC +  GRS +Y+P G F++ +  FSGPC +
Sbjct: 29  PKIFDVRNYGARADSQRDNAFAFTKAWNEACQWSYGRSTVYIPSGIFYLRQVTFSGPCKS 88

Query: 114 DLSPKVLILGTL---RAPTALDDADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNS 173
            ++    I GTL   R P A++  + WI FK + +L +TGG      +LDGQG+ +W  +
Sbjct: 89  SIT--FFIRGTLLAPRNPYAINQEE-WILFKYVDNLTVTGG-----GLLDGQGSYSWPLN 148

Query: 174 ACRRHFTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQ 233
            C ++  C   A ++  + V   +++ +  ++SK  H  L   ++ +++G  I APGDS 
Sbjct: 149 DCNKNTNCRTLAMNIGFAFVKSSKINGLRSINSKMGHFNLFSVEDFNITGVTITAPGDSP 208

Query: 234 NTDGIHVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNE 293
           NTDGI + +S+++ I +  IG GDDC++I  G+ N+ +SD+ CGPGHGISVGSLGRY+ E
Sbjct: 209 NTDGIKIGKSSHMQIYNVTIGTGDDCIAILDGTSNLDISDVRCGPGHGISVGSLGRYKEE 268

Query: 294 MDVVGIRVENCSINGTTNGVRIKTWPGQHSSIA-TNLTFQNIVMSNVSNPIVIDQQYCPN 353
            +V G+ V N  INGTT+G+RIKTW    S I+ +N  ++NI M NV NPIVIDQQYCP+
Sbjct: 269 KNVQGLTVRNSIINGTTDGLRIKTWAKSVSQISVSNFLYENIQMINVGNPIVIDQQYCPH 328

Query: 354 HSCKT--HEASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINLTATQPPD 413
             C +    AS+V++ ++K  NI G+  S+ A+ +QCS    C+ +ELS INL       
Sbjct: 329 GQCDSPGKYASHVQIKDVKYNNIWGTSTSKEALKMQCSKTFPCQDVELSNINL------- 378

[Close]

BLAST of MC05g0648 vs. TAIR 10
Match: AT1G78400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 282.7 bits (722), Expect = 4.9e-76
Identity = 139/348 (39.94%), Postives = 215/348 (61.78%), Query Frame = 0


Query: 55  RIFDVTDYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDL 114
           ++FDV  YGA  DG T++++ F  AW++AC + G   +Y+P GTF++G   F+GPC + +
Sbjct: 33  KVFDVRSYGARGDGKTDNTMAFTKAWKDACQWKGLPRVYIPFGTFYLGAVAFTGPCKSRI 92

Query: 115 SPKVLILGTLRAPTALD--DADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACR 174
           S   +I GTL AP   +    D WI F+ + +L ++GG      ILDGQG+ +W  + CR
Sbjct: 93  S--FIIKGTLLAPKDPNAIKQDSWIIFRYVDYLTVSGG-----GILDGQGSYSWPLNNCR 152

Query: 175 RHFTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTD 234
           +   C     ++    V + R++ I+ ++SK  H+      +  ++  NI+APGDS NTD
Sbjct: 153 QTHNCRALPMNMGFQFVRFSRLTRIKSINSKMGHLNFFSVQHFDITRVNIKAPGDSPNTD 212

Query: 235 GIHVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDV 294
           GI +  S ++ I   DI  GDDC++I  G+ N+ ++ +NCGPGHGISVGSLG+++ E  V
Sbjct: 213 GIKIGSSNHMKIHHVDIATGDDCIAILSGTFNLDINKVNCGPGHGISVGSLGKFKGEKSV 272

Query: 295 VGIRVENCSINGTTNGVRIKTWPGQ-HSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSC 354
            G+ V N   NGT+NGVRIKTWP     ++ +N  F+N+ M +V +PI IDQ+YCPN  C
Sbjct: 273 QGLIVRNSIFNGTSNGVRIKTWPSPGEPNLVSNFLFKNLQMIDVQSPINIDQRYCPNPPC 332

Query: 355 KTHEASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINL 400
                S +++ ++K +NI G+  ++ AV LQCS    C+ ++L  IN+
Sbjct: 333 SFQSFSKIQIRDVKFQNIWGTSTAKEAVKLQCSKNVPCKNVQLFNINI 373

[Close]

BLAST of MC05g0648 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 3.1e-75
Identity = 145/345 (42.03%), Postives = 203/345 (58.84%), Query Frame = 0


Query: 58  DVTDYGAVADGNTESSLEFMVAWEEACAYPGRSVLYVPQGTFFVGETVFSGPCFNDLSPK 117
           DV   GA  DG T+ S  F  AW+EACA    S + VP+G + V    F GPC   ++  
Sbjct: 72  DVKASGAKGDGKTDDSAAFAAAWKEACA--AGSTITVPKGEYLVESLEFKGPCKGPVT-- 131

Query: 118 VLILGTLRAPTALDDADV---WIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSACRRH 177
           + + G  +AP  +        WI F++L    + G      AI DGQG+  W  + C + 
Sbjct: 132 LELNGNFKAPATVKTTKPHAGWIDFENLADFTLNG----NKAIFDGQGSLAWKANDCAKT 191

Query: 178 FTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQNTDGI 237
             C+    +++ + ++  ++++I   +SK FH+ +  C NI L+   I AP +S NTDGI
Sbjct: 192 GKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGI 251

Query: 238 HVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEMDVVG 297
           H+ +S  +N+  + I  GDDCVSIG G+ N+ V ++ CGPGHGIS+GSLGRY NE  V G
Sbjct: 252 HIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKG 311

Query: 298 IRVENCSINGTTNGVRIKTWPGQHSSIATNLTFQNIVMSNVSNPIVIDQQYCPNHSCKTH 357
           + V  C I  T NGVRIKTWPG    IA+N+ F++I M NVS P++IDQ+YCP   CK  
Sbjct: 312 VTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAG 371

Query: 358 EASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINL 400
             S VKLS++ ++NI G+  ++ AV L CS G  C  I LS INL
Sbjct: 372 VPSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINL 408

[Close]

BLAST of MC05g0648 vs. TAIR 10
Match: AT1G43080.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 275.8 bits (704), Expect = 5.9e-74
Identity = 148/356 (41.57%), Postives = 222/356 (62.36%), Query Frame = 0


Query: 54  PRIFDVTDYGAVADGNTESSLEFMVAWEEACA-YPGRSVLYVPQGTFFVGETVFSGPCFN 113
           P++F+V  YG+  DG T+++  F   W+ AC    G S +YVP+GTF++G   F GPC N
Sbjct: 28  PKVFNVRRYGSRPDGKTDNANAFTSVWKRACTRISGSSKIYVPKGTFYLGGVEFVGPCKN 87

Query: 114 DLSPKVLILGTLRAPTALDD--ADVWIHFKSLHHLDITGGDTNTTAILDGQGAQTWSNSA 173
            +  + +I GTL AP    D   D WI+F+ +++L I+G  T     LDGQG  +W  + 
Sbjct: 88  PI--EFVIDGTLLAPANPRDIKQDTWINFRYINNLSISGSGT-----LDGQGKYSWPLND 147

Query: 174 CRRHFTCDIFATSLKLSDVSYGRVSNIEVVDSKGFHVALHGCDNIHLSGFNIRAPGDSQN 233
           C ++  C   A ++  + V+  R+ +I  ++SK  H         +++G  I APGDS N
Sbjct: 148 CHKNTNCPKLAMTMGFAFVNNSRIKDITSLNSKMGHFNFFSVHRFNITGVTITAPGDSPN 207

Query: 234 TDGIHVSQSTNINITSSDIGVGDDCVSIGPGSVNVSVSDINCGPGHGISVGSLGRYQNEM 293
           TDGI +   +NI+I++++IG GDDC++I  G+ N+ +S+I CGPGHGISVGSLG+ ++E 
Sbjct: 208 TDGIKMGSCSNIHISNTNIGTGDDCIAILSGTTNLDISNIKCGPGHGISVGSLGKNKDEK 267

Query: 294 DVVGIRVENCSINGTTNGVRIKTWPGQHSSI-ATNLTFQNIVMSNVSNPIVIDQQYCPNH 353
           DV  + V +   NGT++G+RIKTW    S I  +N  ++NI M +V  PI IDQ+YCP+ 
Sbjct: 268 DVKHLTVRDTVFNGTSDGIRIKTWESSASKIVVSNFIYENIQMIDVGKPINIDQKYCPHP 327

Query: 354 SCKTHE---ASNVKLSEIKMRNISGSYNSEFAVNLQCSSGKACEKIELSYINLTAT 403
            C+ HE    S+V++ +IK++NI G+ N+  AVNLQCS    C+ +EL  INL  T
Sbjct: 328 PCE-HEKKGESHVQIQDIKLKNIYGTSNNIVAVNLQCSKSFPCKNVELIDINLKHT 375

The following BLAST results are available for this feature:

BLAST of MC05g0648 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6H9K0	1.2e-76	41.21	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
Q39766	2.8e-76	41.43	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]
Q39786	1.8e-75	40.99	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]
P35339	3.8e-73	39.61	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]
P49063	8.4e-73	41.45	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ...	[more]

BLAST of MC05g0648 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138060.1	2.15e-263	100.00	exopolygalacturonase-like [Momordica charantia]	[more]
XP_038885913.1	2.15e-210	69.84	exopolygalacturonase-like [Benincasa hispida]	[more]
XP_023539060.1	9.36e-207	69.41	exopolygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
KAG7028995.1	2.68e-206	69.18	Exopolygalacturonase-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_004134495.1	6.38e-187	63.47	exopolygalacturonase [Cucumis sativus]	[more]

BLAST of MC05g0648 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C8D5	1.04e-263	100.00	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111009318 PE=3 SV...	[more]
A0A0A0L932	3.09e-187	63.47	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G164560 PE=3 SV=1	[more]
A0A6J1FMM1	1.61e-176	71.07	exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111446788 PE=3 SV=...	[more]
A0A6J1GXB0	1.27e-175	69.58	exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111457998 PE=3 SV=...	[more]
A0A6J1I855	1.76e-173	70.22	exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470865 PE=3 SV=1	[more]

BLAST of MC05g0648 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18180.1	3.6e-79	45.56	Pectin lyase-like superfamily protein	[more]
AT2G33160.1	4.7e-79	42.23	glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family pro...	[more]
AT1G78400.1	4.9e-76	39.94	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	3.1e-75	42.03	Pectin lyase-like superfamily protein	[more]
AT1G43080.1	5.9e-74	41.57	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 212..238 e-value: 24.0 score: 13.8 coord: 239..260 e-value: 5300.0 score: 2.1 coord: 262..282 e-value: 9900.0 score: 0.1 coord: 292..313 e-value: 8.8 score: 15.3 coord: 322..343 e-value: 1300.0 score: 6.4
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 52..424 e-value: 1.6E-96 score: 325.8
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 84..403 e-value: 5.6E-84 score: 282.0
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 269..282
None	No IPR available	PANTHER	PTHR31375:SF33	EXOPOLYGALACTURONASE-LIKE	coord: 41..405
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 41..405
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..22 score: 5.0
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 50..403

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0648.1	MC05g0648.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity