Homology
BLAST of MC05g0625 vs. ExPASy Swiss-Prot
Match:
Q8RXK8 (Isoleucine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA2 PE=2 SV=1)
HSP 1 Score: 1685.6 bits (4364), Expect = 0.0e+00
Identity = 809/1082 (74.77%), Postives = 939/1082 (86.78%), Query Frame = 0
Query: 23 ATKSLICSSPYRVLSQRISASLRRSS-ISSFSFRGSSSAKLFSFLRVTHYSTYLNDEFPF 82
A +++ SS YRVLS + ++LRR++ + SF +G SS K FSFL V+ +ST N+EF
Sbjct: 16 AAMAMVQSSSYRVLSGKSCSNLRRNTPLDSFLAKGRSSVKAFSFLYVSRFSTEPNNEFGH 75
Query: 83 SSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFGMRANSSVREPEIQKIWDDHQV 142
SSKRRSRGPVMAA+KAS+ + DGKYK TVDLPKT FGMRANS REPE+QK+W+++QV
Sbjct: 76 SSKRRSRGPVMAAKKASEGEKQEDGKYKQTVDLPKTGFGMRANSLTREPELQKLWEENQV 135
Query: 143 FNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVRFVPGWDCH 202
F RV + N G +FILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKV++VPGWDCH
Sbjct: 136 FKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWDCH 195
Query: 203 GLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMISFKVQFLQRYGVWADWNN 262
GLPI+ VLQSLD+E RK+LTP+KLR KAAKFAKATVK QM SFK R+GVWADWNN
Sbjct: 196 GLPIELKVLQSLDQEVRKELTPLKLRAKAAKFAKATVKTQMESFK-----RFGVWADWNN 255
Query: 263 PYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIY 322
PYLTL PEYEAAQIEVFGQMA++GYI+RGRKPVHWSPSSRTALAEAELEYPEGH S+SIY
Sbjct: 256 PYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIY 315
Query: 323 AIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLQYAIVELRAPSED 382
AIF+LV G LL EF+P++ LA+WTTTPWT+PANAAVAVNAKLQY++VE+++ SED
Sbjct: 316 AIFKLV--GGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSFSED 375
Query: 383 VSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWGMKLVVKKKLLGSELENCRYIH 442
S + NKK+ PG +LK ++K F++VA+DLVP LEAKWG+KL + K LGS+LENCRY H
Sbjct: 376 ESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCRYTH 435
Query: 443 PIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTGMKYGLPLISPVDDDGKFTEEA 502
PID R+C VVIGGDYITTE+GTGLVHTAPGHGQEDY TG+KYGLPL+SPVDD+GKFTEEA
Sbjct: 436 PIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEA 495
Query: 503 GQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYDWRTKKPTIFRATEQWFASVEG 562
GQF GL VLG+GNTAVV YLD+ SL++EESY HKYPYDWRTKKPTIFRATEQWFASVEG
Sbjct: 496 GQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEG 555
Query: 563 FRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQRTWGVPIPVFYHVETKEPLMND 622
FR A MDAI VKW+P QA NRIS MTSSRSDWCISRQRTWGVPIP FYHV+TKEPLMN+
Sbjct: 556 FRTATMDAINNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNE 615
Query: 623 DTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYEKGTDTMDVWFDSGSSWAAVLG 682
+TINH+KSII++KGSDAWWYM V+DLLP+KYR+ A++YEKGTDTMDVWFDSGSSWA VLG
Sbjct: 616 ETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDVWFDSGSSWAGVLG 675
Query: 683 TQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVITHGFVLDEKGLKMSKSL 742
++G SFPAD+YLEGTDQHRGWFQSSLLTSIAT+GKAPYS+VITHGFVLDEKG+KMSKSL
Sbjct: 676 KREGLSFPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSL 735
Query: 743 GNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGDVMIGSQVLRQMSDIYRKLRGT 802
GNVVDP+ VIEGGKN K+ P YGADV+RLWVSSVDYTGDV+IG Q+LRQMSDIYRKLRGT
Sbjct: 736 GNVVDPRLVIEGGKNSKDAPAYGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRGT 795
Query: 803 LRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINNIRESYESYQFCKIFQIIQRFV 862
LR+LLGNLHDW DN V YQDLP IDQHALFQLENV+ NI+E YE+YQF KIFQIIQRF
Sbjct: 796 LRYLLGNLHDWRVDNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFT 855
Query: 863 IVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVSITRIIAPILPHLAEDVWQNLP 922
IVDLSNFYFD+AKDRLY GG++SFTRRSCQTVL+ HL+SI R+IAPI+PHLAEDVWQNLP
Sbjct: 856 IVDLSNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLP 915
Query: 923 FEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWANILELRTEVNKVLEVARTEKLI 982
FE++N+DGS A+FVFE +WP+LN+ L P E+V FW +LELRTEVNKVLE+AR EK+I
Sbjct: 916 FEYRNEDGSAAEFVFELKWPTLNEQWLSFPAEDVLFWQRLLELRTEVNKVLELARNEKMI 975
Query: 983 GSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFITSQAEVHRSIENELVENIPYT 1042
GSSLEAKV+LHT+ G+ +KL EM E+ +EADTLQRIFITSQ EV S+E E++ ++ +T
Sbjct: 976 GSSLEAKVYLHTADAGMAAKLLEMSEAKNEADTLQRIFITSQVEVLSSMEKEMISSVQHT 1035
Query: 1043 GECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHPTLCKRCFDV-VAGHPEPAMAA 1102
GE + +KVWIGVSRAEGSKCERCWN+S +VGSF +HPTLC RCF V VA PEPA+AA
Sbjct: 1036 GEYVEGENKVWIGVSRAEGSKCERCWNYSGQVGSFSDHPTLCGRCFSVIVANPPEPAVAA 1090
BLAST of MC05g0625 vs. ExPASy Swiss-Prot
Match:
Q8YXY3 (Isoleucine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=ileS PE=3 SV=1)
HSP 1 Score: 1169.1 bits (3023), Expect = 0.0e+00
Identity = 570/989 (57.63%), Postives = 721/989 (72.90%), Query Frame = 0
Query: 106 GKYKHTVDLPKTTFGMRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANG 165
G YK TV+LPKT F MRAN+ REPEIQK W+++++F + + N GE FILHDGPPYANG
Sbjct: 5 GSYKDTVNLPKTNFDMRANAIKREPEIQKFWEENKIFESLSQNNPGELFILHDGPPYANG 64
Query: 166 DLHMGHALNKILKDIINRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIK 225
LH+GHALNKILKDIINRY+LLQ KVR+VPGWDCHGLPI+ VLQ+L R++LTP++
Sbjct: 65 SLHIGHALNKILKDIINRYQLLQGRKVRYVPGWDCHGLPIELKVLQNLKSAERQNLTPLQ 124
Query: 226 LRDKAAKFAKATVKAQMISFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQG 285
LR KA +FA ATV Q +FK RYGVW DW+NPYLTL PEYEAAQI VFGQM ++G
Sbjct: 125 LRQKAKEFALATVDNQRQNFK-----RYGVWGDWDNPYLTLKPEYEAAQIGVFGQMVLKG 184
Query: 286 YIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLC 345
YI+RG KPVHWSPSS+TALAEAELEYPEGHTSRSIYA F + + LL E+LPDL
Sbjct: 185 YIYRGLKPVHWSPSSKTALAEAELEYPEGHTSRSIYAAFPVTSFAEAAKPLLGEYLPDLG 244
Query: 346 LAIWTTTPWTIPANAAVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFL 405
+AIWTTTPWTIP N AVAVN L Y++VE+ + + +L
Sbjct: 245 VAIWTTTPWTIPGNLAVAVNGDLNYSLVEVSRIGAET----------------QSNFKYL 304
Query: 406 VVASDLVPTLEAKWGMKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGL 465
+VA+DLV L A +L VK G ELE+ Y HP+ RE VV+GGDYITTE+GTGL
Sbjct: 305 IVAADLVERLAATISAQLTVKATFKGKELEHTTYRHPLFDRESPVVVGGDYITTESGTGL 364
Query: 466 VHTAPGHGQEDYVTGMKYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQF 525
VHTAPGHGQEDYV G++YGLP+++PVDD+G FT+EAGQF+GL+VLG+GN AV+ L
Sbjct: 365 VHTAPGHGQEDYVVGLRYGLPILAPVDDNGDFTQEAGQFAGLNVLGEGNQAVIDALTAAG 424
Query: 526 SLILEESYEHKYPYDWRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRIS 585
SL+ EE+Y HKYPYDWRTKKPTIFRATEQWFASVEGFR A+ AI VKWIP Q ENRI+
Sbjct: 425 SLLKEEAYAHKYPYDWRTKKPTIFRATEQWFASVEGFRDEALKAIAAVKWIPAQGENRIT 484
Query: 586 VMTSSRSDWCISRQRTWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVD 645
M + RSDWCISRQR+WGVPIPVFY ET EPL+N++TIN++++IIA+KGSDAWW + V
Sbjct: 485 PMVAERSDWCISRQRSWGVPIPVFYDEETGEPLLNEETINYVQAIIAEKGSDAWWELSVA 544
Query: 646 DLLPDKYREIASEYEKGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQ 705
+LLP+ YR Y +GTDTMDVWFDSGSSWA+V+ + +PAD+YLEG+DQHRGWFQ
Sbjct: 545 ELLPESYRNNGRSYRRGTDTMDVWFDSGSSWASVVKQRPELRYPADMYLEGSDQHRGWFQ 604
Query: 706 SSLLTSIATKGKAPYSSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGA 765
SSLLTS++ G APY +V+THGFVLDE+G KMSKS GNVVDP +I GGK+QK+ P YGA
Sbjct: 605 SSLLTSVSVNGIAPYKTVLTHGFVLDEQGRKMSKSEGNVVDPAIIINGGKDQKKEPPYGA 664
Query: 766 DVLRLWVSSVDYTGDVMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDW-TEDNYVQYQDLP 825
DV+RLW SSVDYTGDV +G +++Q++D+ K+R T RFLLG+L+D+ E N VQ++++P
Sbjct: 665 DVMRLWASSVDYTGDVRLGGNIIKQLNDVRGKIRNTARFLLGSLYDFDPEKNAVQFEEMP 724
Query: 826 KIDQHALFQLENVINNIRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTS 885
++D++ L ++ V + E++ES+QF + FQ +Q F +VDLSNFY DVAKDRLY+ +
Sbjct: 725 QLDKYMLHRIREVFEEVTEAFESFQFFRFFQTVQNFCVVDLSNFYLDVAKDRLYISAPDA 784
Query: 886 FTRRSCQTVLADHLVSITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLN 945
F RRSCQTV+ L ++ R IAP+L H AED+WQ LP++ + K VFE+ W +
Sbjct: 785 FRRRSCQTVIHIALENLARAIAPVLCHTAEDIWQYLPYK------TPYKSVFEAGWVQVE 844
Query: 946 KTRLLPREEVDFWANILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEM 1005
+ P E +FW + +LRT+VNKVLE AR EK+IGSSLEAK ++ ++ +
Sbjct: 845 EKWHNP-ELAEFWQQLRQLRTDVNKVLEQARVEKMIGSSLEAKALIYVKDANSRKAIATL 904
Query: 1006 -CESNHEADTLQRIFITSQAEVHRSIENELVENIPYTGECLIRGSKVW-IGVSRAEGSKC 1065
E + D L+ +F+TSQ E+ S + +++ YT + S W IGV AEG KC
Sbjct: 905 NPEVGNGVDELRYLFLTSQVELLDSADK--LQDGKYTSQ-----SDSWGIGVVNAEGQKC 958
Query: 1066 ERCWNFSLRVGSFLEHPTLCKRCFDVVAG 1092
+RCWN+S VG EHP LC+RC +AG
Sbjct: 965 DRCWNYSTHVGESAEHPLLCERCVPALAG 958
BLAST of MC05g0625 vs. ExPASy Swiss-Prot
Match:
B7K5H5 (Isoleucine--tRNA ligase OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=ileS PE=3 SV=1)
HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 564/989 (57.03%), Postives = 720/989 (72.80%), Query Frame = 0
Query: 108 YKHTVDLPKTTFGMRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDL 167
YK TV+LP+T F MRAN+ REPEIQ+ W ++ ++ ++ + N + FILHDGPPYANG L
Sbjct: 7 YKDTVNLPQTDFSMRANAVQREPEIQQFWTENCIYEQLSQNNPEDLFILHDGPPYANGSL 66
Query: 168 HMGHALNKILKDIINRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLR 227
HMGHALNK LKDIIN+YKLL+ +KVR+VPGWDCHGLPI+ VLQS+ E R+ LTP+KLR
Sbjct: 67 HMGHALNKTLKDIINKYKLLRGHKVRYVPGWDCHGLPIELKVLQSMKSEEREGLTPLKLR 126
Query: 228 DKAAKFAKATVKAQMISFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYI 287
KA FA T + Q FK RYGVW DW NPYLTL+PEYEAAQI VFGQMA++GYI
Sbjct: 127 RKARDFALKTQQEQAEGFK-----RYGVWGDWENPYLTLTPEYEAAQIGVFGQMALKGYI 186
Query: 288 FRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLA 347
+RG KPVHWSPSSRTALAEAELEYPEGHTS+SI+A F ++K + +L FL +L +A
Sbjct: 187 YRGLKPVHWSPSSRTALAEAELEYPEGHTSQSIFAAFPIIKSSKDAQEILDPFLSNLGVA 246
Query: 348 IWTTTPWTIPANAAVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVV 407
IWTTTPWT+P N AVA+N +L YAIVE + N+ + +++V
Sbjct: 247 IWTTTPWTLPGNLAVALNPELTYAIVE-----------------QTSNLCNYQ---YIIV 306
Query: 408 ASDLVPTLEAKWGMKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVH 467
A+DLV L A + +L VK L G LE+ Y HP+ RE ++VIGGDY+TTE+GTGLVH
Sbjct: 307 AADLVERLSATFSTELTVKATLPGQILEHTIYRHPLYDRESEIVIGGDYVTTESGTGLVH 366
Query: 468 TAPGHGQEDYVTGMKYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSL 527
TAPGHGQEDY+ G +YGL ++SPVDD G FTEEAGQFSGL+VL D N A++ L ++ SL
Sbjct: 367 TAPGHGQEDYIVGQRYGLQVLSPVDDAGNFTEEAGQFSGLNVLKDANQAIINELKNKGSL 426
Query: 528 ILEESYEHKYPYDWRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVM 587
+ EE Y HKYPYDWRTKKPTIFRATEQWFASVEGFR+AA++AI V WIPPQ ENRI+ M
Sbjct: 427 LKEEPYLHKYPYDWRTKKPTIFRATEQWFASVEGFREAALEAIKSVNWIPPQGENRITPM 486
Query: 588 TSSRSDWCISRQRTWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDL 647
S RSDWCISRQR+WGVPIPVFY+ ET EPL+ ++TINH+++IIAK+GSDAWW + +++L
Sbjct: 487 VSDRSDWCISRQRSWGVPIPVFYNEETNEPLLTEETINHVQAIIAKQGSDAWWELSIEEL 546
Query: 648 LPDKYREIASEYEKGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSS 707
LP++Y++ A +Y +GTDTMDVWFDSGSSWAAV ++ +P D+YLEG+DQHRGWFQSS
Sbjct: 547 LPEQYKKDAHKYRRGTDTMDVWFDSGSSWAAVAKQREELKYPVDIYLEGSDQHRGWFQSS 606
Query: 708 LLTSIATKGKAPYSSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADV 767
LLTS+A G APY +V+THGFVLDEKG KMSKSLGN+VDP +I GGKNQK+ P YGADV
Sbjct: 607 LLTSVAVNGIAPYKTVLTHGFVLDEKGHKMSKSLGNIVDPLVIINGGKNQKQEPPYGADV 666
Query: 768 LRLWVSSVDYTGDVMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDW-TEDNYVQYQDLPKI 827
LRLWVSSVDY+ DV IG +L+Q+SD+YRK+R T RFLLGNLHD+ E + V Y LP++
Sbjct: 667 LRLWVSSVDYSSDVPIGQTILKQLSDVYRKIRNTARFLLGNLHDFDPEKDTVSYDQLPEL 726
Query: 828 DQHALFQLENVINNIRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFT 887
DQ+ L ++ V + +++E +QF + FQ +Q F +VDLSNFY D+AKDRLY+ + S
Sbjct: 727 DQYMLHRITEVFTEVTDAFEKFQFFRFFQTVQNFCVVDLSNFYLDIAKDRLYISDTNSLR 786
Query: 888 RRSCQTVLADHLVSITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKT 947
RRSCQTVL + S+ + IAP+L H+AED+WQ LP++ + + VF S W + K
Sbjct: 787 RRSCQTVLKVAVESLAKAIAPVLCHMAEDIWQFLPYK------TPYQSVFASGWVEMQKQ 846
Query: 948 RLLPREEVDFWANILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCE 1007
P E W + ++RTEVNKVLE AR EK IGSSL+AKV L+ S +L
Sbjct: 847 WERP-ELTASWGKLRQIRTEVNKVLEQARNEKAIGSSLDAKVLLYVSDQDFKKQLESFNP 906
Query: 1008 SN----HEADTLQRIFITSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKC 1067
++ ++ D L+ + + SQ E+ S+ E ++ Y E V +GV +AEG KC
Sbjct: 907 NDSLKGNQVDELRYLVLASQVELVDSL--EAIKKADYQSE----SELVSVGVVKAEGQKC 957
Query: 1068 ERCWNFSLRVGSFLEHPTLCKRCFDVVAG 1092
+RCWN+S +VG F + PT+C+RC + G
Sbjct: 967 DRCWNYSTKVGEFSDDPTICERCNAALVG 957
BLAST of MC05g0625 vs. ExPASy Swiss-Prot
Match:
B7KFT1 (Isoleucine--tRNA ligase OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=ileS PE=3 SV=1)
HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 557/991 (56.21%), Postives = 716/991 (72.25%), Query Frame = 0
Query: 108 YKHTVDLPKTTFGMRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDL 167
YK TV+LP+T F MRAN+ REPE+QK W ++Q++ + E N G+ FILHDGPPYANG L
Sbjct: 7 YKDTVNLPQTKFDMRANAVKREPELQKFWAENQIYETLSENNPGDIFILHDGPPYANGSL 66
Query: 168 HMGHALNKILKDIINRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLR 227
HMGHALNK LKDIIN+YKL + YK R+VPGWDCHGLPI+ VLQS+ ++ R+DLTP+KLR
Sbjct: 67 HMGHALNKTLKDIINKYKLQRGYKARYVPGWDCHGLPIELKVLQSMKQKEREDLTPLKLR 126
Query: 228 DKAAKFAKATVKAQMISFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYI 287
KA FA +T K Q SF QR+GVW DW +PYLTL+P+YEAAQI VFGQMA++GYI
Sbjct: 127 HKARDFALSTQKEQSESF-----QRFGVWGDWEHPYLTLTPDYEAAQIGVFGQMALKGYI 186
Query: 288 FRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLA 347
+RG KPVHWSPSS+TALAEAELEYPEGHTSRS+YA+F + K + +L +L DL +A
Sbjct: 187 YRGLKPVHWSPSSQTALAEAELEYPEGHTSRSVYAVFPITKASDTAKKVLTPYLKDLGVA 246
Query: 348 IWTTTPWTIPANAAVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVV 407
IWTTTPWT+P N AVA+N L YA+VE P + K + + +V
Sbjct: 247 IWTTTPWTLPGNLAVALNPDLTYAVVESGKP-----------------VCKHQ---YFIV 306
Query: 408 ASDLVPTLEAKWGMKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVH 467
A+DLV L A + L VK L G +LE+ Y HP+ RE +++IGGDY+TTE+GTGLVH
Sbjct: 307 AADLVDKLSATFETPLTVKATLKGEDLEHTTYRHPLFDRESEILIGGDYVTTESGTGLVH 366
Query: 468 TAPGHGQEDYVTGMKYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSL 527
TAPGHGQEDY+ G +YGLP++SPVD+ G FT EAGQF+GL+VL D N A++K L+++ +L
Sbjct: 367 TAPGHGQEDYIVGQRYGLPVLSPVDEKGNFTSEAGQFAGLNVLKDANEAIIKELEEKGAL 426
Query: 528 ILEESYEHKYPYDWRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVM 587
+ EE Y+HKYPYDWRTKKPTIFRATEQWFASVEGFR A++AI V WIPPQ ENRI+ M
Sbjct: 427 LKEEPYQHKYPYDWRTKKPTIFRATEQWFASVEGFRDTALEAIKNVTWIPPQGENRITPM 486
Query: 588 TSSRSDWCISRQRTWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDL 647
+ RSDWCISRQR+WGVPIPVFY ET EPL+N++TINH+++I A+KGSDAWW + V++L
Sbjct: 487 VADRSDWCISRQRSWGVPIPVFYDEETNEPLLNEETINHVQAIFAEKGSDAWWELSVEEL 546
Query: 648 LPDKYREIASEYEKGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSS 707
LP YR +Y KG DTMDVWFDSGSSWAAV + S+P D+YLEG+DQHRGWFQSS
Sbjct: 547 LPQPYRNNGRKYRKGMDTMDVWFDSGSSWAAVAKQRPELSYPVDIYLEGSDQHRGWFQSS 606
Query: 708 LLTSIATKGKAPYSSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADV 767
LLTS+AT G APY V+THGFVLDE+G KMSKS+GN+VDP +I GGK+QK+ P YGADV
Sbjct: 607 LLTSVATNGIAPYKMVLTHGFVLDEQGRKMSKSIGNIVDPNMIINGGKDQKKEPAYGADV 666
Query: 768 LRLWVSSVDYTGDVMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDW-TEDNYVQYQDLPKI 827
LRLWVSSVDY+ DV IG +L+Q+ D+ K+R T RFLLGNLHD+ E + V Y+DLP++
Sbjct: 667 LRLWVSSVDYSSDVPIGQNILKQLVDVRNKIRNTARFLLGNLHDFDPEKDTVAYEDLPEL 726
Query: 828 DQHALFQLENVINNIRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFT 887
D++ L ++ V + E++E++QF + FQ++Q F +VDLSNFY D+AKDRLY+ +
Sbjct: 727 DRYMLHRITEVFTEVTEAFETFQFFRFFQVVQNFCVVDLSNFYLDIAKDRLYISDPNNLR 786
Query: 888 RRSCQTVLADHLVSITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKT 947
RRSCQTVL L ++ + IAP+L HLAED+WQ LP++ + K VFE+ W L
Sbjct: 787 RRSCQTVLKVALENLAKSIAPVLCHLAEDIWQFLPYK------TPYKSVFEAGWVELESE 846
Query: 948 RLLPREEVDFWANILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEM-- 1007
P E W+ ++R EVNKV+E+AR +K+IGSSL+AKV L+ S L L
Sbjct: 847 WKRP-ELTPKWSKFRQIRDEVNKVMELARKDKMIGSSLDAKVLLYVSDKELREHLDSFNP 906
Query: 1008 --CESNHEADTLQRIFITSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVS--RAEGS 1067
SN+ D L+ +F+ SQ +LV++ G+ + +GV+ +A+G
Sbjct: 907 SDSLSNNRVDELRYLFLASQV--------DLVDSPKTIGKANYKSESETLGVAIVKADGE 957
Query: 1068 KCERCWNFSLRVGSFLEHPTLCKRCFDVVAG 1092
KC+RCWN+S VG F++ PTLC+RC + G
Sbjct: 967 KCDRCWNYSTHVGEFVDDPTLCERCNAALKG 957
BLAST of MC05g0625 vs. ExPASy Swiss-Prot
Match:
B2IZP5 (Isoleucine--tRNA ligase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=ileS PE=3 SV=1)
HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 563/994 (56.64%), Postives = 719/994 (72.33%), Query Frame = 0
Query: 106 GKYKHTVDLPKTTFGMRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANG 165
G YK TV+LPKT+F MRAN+ REPEIQK W+++++++R+ E N GE FILHDGPPYANG
Sbjct: 5 GSYKDTVNLPKTSFDMRANAIKREPEIQKFWEENKIYDRLFENNPGELFILHDGPPYANG 64
Query: 166 DLHMGHALNKILKDIINRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIK 225
LH+GHALNKILKDIINRY++L+ KVR+VPGWDCHGLPI+ VLQ++ R++LTP++
Sbjct: 65 SLHIGHALNKILKDIINRYQMLRGRKVRYVPGWDCHGLPIELKVLQNMKSAERQNLTPLQ 124
Query: 226 LRDKAAKFAKATVKAQMISFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQG 285
LR KA +F ATV Q +FK RYG+W DW+NPYLTL PEYEAAQI VFGQM ++G
Sbjct: 125 LRQKAKEFGLATVDNQRQNFK-----RYGIWGDWDNPYLTLKPEYEAAQIGVFGQMFLKG 184
Query: 286 YIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLC 345
YI+RG KPVHWSPSS+TALAEAELEYPEGH SRSIYA F + LL E+ DL
Sbjct: 185 YIYRGLKPVHWSPSSKTALAEAELEYPEGHVSRSIYAAFPVTSLAEAVKPLLAEYQSDLG 244
Query: 346 LAIWTTTPWTIPANAAVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFL 405
+AIWTTTPWTIP N AVAVNA L YA+VE VS S K + +L
Sbjct: 245 VAIWTTTPWTIPGNLAVAVNADLNYAVVE-------VSQSEAQSKFK-----------YL 304
Query: 406 VVASDLVPTLEAKWGMKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGL 465
+VA+DLV L + G++L +K G++LE+ Y HP+ RE +V+GGDYITTE+GTGL
Sbjct: 305 IVAADLVERLSSTLGLELTLKATFKGNDLEHTTYRHPLFDRESPIVVGGDYITTESGTGL 364
Query: 466 VHTAPGHGQEDYVTGMKYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQF 525
VHTAPGHGQEDY+ G +YGLP+++PVDD+G FT+EAG+F+GL+VLGDGN AV+ L
Sbjct: 365 VHTAPGHGQEDYIVGQRYGLPILAPVDDNGNFTQEAGEFAGLNVLGDGNQAVIDALAAAG 424
Query: 526 SLILEESYEHKYPYDWRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRIS 585
SL+ EE Y HKYPYDWRTKKPTIFRATEQWFASVEGFR+ A+ AI VKWIP Q ENRI+
Sbjct: 425 SLLKEEPYPHKYPYDWRTKKPTIFRATEQWFASVEGFREEALKAIATVKWIPAQGENRIT 484
Query: 586 VMTSSRSDWCISRQRTWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVD 645
M + RSDWCISRQR+WGVPIPVFY T EPL+N++ INH++ IIA+KGSDAWW + V+
Sbjct: 485 PMVAERSDWCISRQRSWGVPIPVFYDEATGEPLLNEEIINHVQGIIAEKGSDAWWELSVE 544
Query: 646 DLLPDKYREIASEYEKGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQ 705
+LLP+ YR+ Y +GTDTMDVWFDSGSSWA+V+ + +PAD+YLEG+DQHRGWFQ
Sbjct: 545 ELLPESYRQNGKSYRRGTDTMDVWFDSGSSWASVVQQRPELRYPADIYLEGSDQHRGWFQ 604
Query: 706 SSLLTSIATKGKAPYSSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGA 765
SSLLTS+A APY +V+THGF LDE+G KMSKS GNVVDP T+IEGGKNQK P YGA
Sbjct: 605 SSLLTSVAVNDIAPYKTVLTHGFALDEQGRKMSKSEGNVVDPNTIIEGGKNQKVEPAYGA 664
Query: 766 DVLRLWVSSVDYTGDVMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDW-TEDNYVQYQDLP 825
DVLRLWVSSVDY+GDV IG +++QM+D+ K+R T RFLLG+L D+ E + V +++LP
Sbjct: 665 DVLRLWVSSVDYSGDVRIGKNIIKQMNDVRGKIRNTARFLLGSLDDFDPEKDTVPFEELP 724
Query: 826 KIDQHALFQLENVINNIRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTS 885
++D++ L ++ V + E++ES+QF + FQ +Q F +VDLSNFY DVAKDRLY+ +
Sbjct: 725 ELDRYMLHRITEVFEEVTEAFESFQFFRFFQTVQNFCVVDLSNFYLDVAKDRLYISAKDA 784
Query: 886 FTRRSCQTVLADHLVSITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLN 945
F RRSCQTVL L ++ R IAP+L H AED+WQ LP++ + K VFE+ W +
Sbjct: 785 FRRRSCQTVLKIALDNLARAIAPVLSHTAEDIWQYLPYK------TPYKSVFEAGWVQVE 844
Query: 946 KTRLLPREEVDFWANILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEM 1005
+ P E +FW+ + ++R +VNK LE+ R +K IGSSLEAKV +H + L +L +
Sbjct: 845 EKWRNP-ELAEFWSELRKIRDKVNKTLEIYRLQKTIGSSLEAKVVVHVFDERLRQRLQAL 904
Query: 1006 -------CESNHEADTLQRIFITSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRA 1065
E ++ D L+ +F+ SQ EV E I G+ + ++I V +A
Sbjct: 905 NPNTHGSVEKSNGVDELRYLFLASQVEVPS-------EPIFEEGD----DAGIYIQVYKA 957
Query: 1066 EGSKCERCWNFSLRVGSFLEHPTLCKRCFDVVAG 1092
+G KC+RCWN+S VG EHP +C+RC +AG
Sbjct: 965 DGEKCDRCWNYSTHVGESAEHPLICERCVAALAG 957
BLAST of MC05g0625 vs. NCBI nr
Match:
XP_022137948.1 (isoleucine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])
HSP 1 Score: 2137 bits (5536), Expect = 0.0
Identity = 1062/1102 (96.37%), Postives = 1072/1102 (97.28%), Query Frame = 0
Query: 1 MEATMPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSA 60
MEATMPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSA
Sbjct: 1 MEATMPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSA 60
Query: 61 KLFSFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFG 120
KLFSFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFG
Sbjct: 61 KLFSFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFG 120
Query: 121 MRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 180
MRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDI
Sbjct: 121 MRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 180
Query: 181 INRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 240
INRYKLLQNYKVRFVPGWDCHGLPI+ VLQSLDKEARKDLTPIKLRDKAAKFAKATVKA
Sbjct: 181 INRYKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 240
Query: 241 QMISFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 300
QMISFK RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS
Sbjct: 241 QMISFK-----RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 300
Query: 301 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANA 360
RTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANA
Sbjct: 301 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANA 360
Query: 361 AVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWG 420
AVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWG
Sbjct: 361 AVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWG 420
Query: 421 MKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTG 480
MKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTG
Sbjct: 421 MKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTG 480
Query: 481 MKYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYD 540
MKYGLPLISPVDDDGKFTEE + L + ++++ +F ++ EHKYPYD
Sbjct: 481 MKYGLPLISPVDDDGKFTEEGEKMIILIPTRFLSFLFLRFISVRFQYVISSYTEHKYPYD 540
Query: 541 WRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQR 600
WRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQR
Sbjct: 541 WRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQR 600
Query: 601 TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYE 660
TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYE
Sbjct: 601 TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYE 660
Query: 661 KGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPY 720
KGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPY
Sbjct: 661 KGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPY 720
Query: 721 SSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGD 780
SSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGD
Sbjct: 721 SSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGD 780
Query: 781 VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINN 840
VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINN
Sbjct: 781 VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINN 840
Query: 841 IRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVS 900
IRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVS
Sbjct: 841 IRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVS 900
Query: 901 ITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWAN 960
ITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL LPREEVDFWAN
Sbjct: 901 ITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRLSLPREEVDFWAN 960
Query: 961 ILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFI 1020
ILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFI
Sbjct: 961 ILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFI 1020
Query: 1021 TSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHP 1080
TSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHP
Sbjct: 1021 TSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHP 1080
Query: 1081 TLCKRCFDVVAGHPEPAMAAIS 1101
TLCKRCFDVVAGHPEPAMAAIS
Sbjct: 1081 TLCKRCFDVVAGHPEPAMAAIS 1097
BLAST of MC05g0625 vs. NCBI nr
Match:
XP_022956209.1 (isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])
HSP 1 Score: 2050 bits (5310), Expect = 0.0
Identity = 1014/1098 (92.35%), Postives = 1043/1098 (94.99%), Query Frame = 0
Query: 5 MPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSAKLFS 64
MP YAS A SSA KCK ATKSLI +S Y VLSQRISASL RS I SF FRGSSSAK+F+
Sbjct: 6 MPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISASLGRSYIPSFRFRGSSSAKVFT 65
Query: 65 FLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFGMRAN 124
FL VT YSTYLNDEF SSKRR+RGPVMAA+KASKET EGDGKYKHTVDLPKTTFGMRAN
Sbjct: 66 FLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRAN 125
Query: 125 SSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRY 184
S+VREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRY
Sbjct: 126 STVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRY 185
Query: 185 KLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMIS 244
KLLQNYKVRFVPGWDCHGLPI+ VLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQM S
Sbjct: 186 KLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMAS 245
Query: 245 FKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL 304
FK RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL
Sbjct: 246 FK-----RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL 305
Query: 305 AEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANAAVAV 364
AEAELEYPEGHTSRSIYAIFRLVKEGP S G+LQEFLPDLCLAIWTTTPWTIPANAAVAV
Sbjct: 306 AEAELEYPEGHTSRSIYAIFRLVKEGPSSGGMLQEFLPDLCLAIWTTTPWTIPANAAVAV 365
Query: 365 NAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWGMKLV 424
NAKL YAIVEL APSEDVS SGNKK+RPGNIL EE +PFLVVASDLVPTLEAKWG+KLV
Sbjct: 366 NAKLHYAIVELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKLV 425
Query: 425 VKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTGMKYG 484
VKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDY TG+KYG
Sbjct: 426 VKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYATGLKYG 485
Query: 485 LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYDWRTK 544
LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDD FSLILEESYEHKYPYDWRTK
Sbjct: 486 LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDHFSLILEESYEHKYPYDWRTK 545
Query: 545 KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQRTWGV 604
KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRIS MTSSRSDWCISRQRTWGV
Sbjct: 546 KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISAMTSSRSDWCISRQRTWGV 605
Query: 605 PIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYEKGTD 664
PIPVFYHV+TKEPLMNDDT+NHIKSIIAKKGSDAWWYM V+DLLPDKYREIASEYEKGTD
Sbjct: 606 PIPVFYHVQTKEPLMNDDTVNHIKSIIAKKGSDAWWYMTVEDLLPDKYREIASEYEKGTD 665
Query: 665 TMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI 724
TMDVWFDSGSSWAAVLGT+DGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI
Sbjct: 666 TMDVWFDSGSSWAAVLGTRDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI 725
Query: 725 THGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGDVMIG 784
THGFVLDEKGLKMSKSLGNVVDPK+VIEGGKNQKE PGYGADVLRLWVSSVDYTGDVMIG
Sbjct: 726 THGFVLDEKGLKMSKSLGNVVDPKSVIEGGKNQKEAPGYGADVLRLWVSSVDYTGDVMIG 785
Query: 785 SQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINNIRES 844
SQVLRQMSDIYRKLRGTLRFLLGNLHDWTE+N VQYQDLP+IDQHALFQLEN+INNIRES
Sbjct: 786 SQVLRQMSDIYRKLRGTLRFLLGNLHDWTEENAVQYQDLPRIDQHALFQLENIINNIRES 845
Query: 845 YESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVSITRI 904
YESYQF KIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLA H+VSI RI
Sbjct: 846 YESYQFFKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLAAHIVSIARI 905
Query: 905 IAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWANILEL 964
IAPILPHLAEDVWQNLPF++ NDDGS AKFVFESRWPSLNKTRL LPREEVDFWANILEL
Sbjct: 906 IAPILPHLAEDVWQNLPFQYTNDDGSAAKFVFESRWPSLNKTRLSLPREEVDFWANILEL 965
Query: 965 RTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFITSQA 1024
RTEVNKVLEVART KLIGSSLEAKVHLH SGDGL +KL EMCESNH+ADTLQRIFITSQ
Sbjct: 966 RTEVNKVLEVARTGKLIGSSLEAKVHLHASGDGLATKLCEMCESNHDADTLQRIFITSQV 1025
Query: 1025 EVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHPTLCK 1084
EVH++IENEL EN PYTGECLI GSKVWIGVSRAEGSKCERCWNFSLRVGSF EHPTLCK
Sbjct: 1026 EVHQAIENELTENTPYTGECLIEGSKVWIGVSRAEGSKCERCWNFSLRVGSFPEHPTLCK 1085
Query: 1085 RCFDVVAGHPEPAMAAIS 1101
RCFDVVAG+PEPAMAA+S
Sbjct: 1086 RCFDVVAGYPEPAMAAVS 1098
BLAST of MC05g0625 vs. NCBI nr
Match:
KAG6582211.1 (Isoleucine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2046 bits (5302), Expect = 0.0
Identity = 1013/1098 (92.26%), Postives = 1040/1098 (94.72%), Query Frame = 0
Query: 5 MPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSAKLFS 64
MP YAS A SSA KCK ATKSLI +S Y VLSQRISA L RS I SF FRGSSSAK+F
Sbjct: 6 MPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISAPLGRSYIPSFRFRGSSSAKVFM 65
Query: 65 FLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFGMRAN 124
FL VT YSTYLNDEF SSKRR+RGPVMAA+KASKET EGDGKYKHTVDLPKTTFGMRAN
Sbjct: 66 FLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRAN 125
Query: 125 SSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRY 184
S+VREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRY
Sbjct: 126 STVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRY 185
Query: 185 KLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMIS 244
KLLQNYKVRFVPGWDCHGLPI+ VLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQM S
Sbjct: 186 KLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMAS 245
Query: 245 FKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL 304
FK RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL
Sbjct: 246 FK-----RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL 305
Query: 305 AEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANAAVAV 364
AEAELEYPEGHTSRSIYAIFRLVKEGP S G+LQEFLPDLCLAIWTTTPWTIPANAAVAV
Sbjct: 306 AEAELEYPEGHTSRSIYAIFRLVKEGPSSGGMLQEFLPDLCLAIWTTTPWTIPANAAVAV 365
Query: 365 NAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWGMKLV 424
NAKL YAIVEL APSEDVS SGNKK+RPGNIL EE +PFLVVASDLVPTLEAKWG+KLV
Sbjct: 366 NAKLHYAIVELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKLV 425
Query: 425 VKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTGMKYG 484
VKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDY TG+KYG
Sbjct: 426 VKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYATGLKYG 485
Query: 485 LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYDWRTK 544
LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDD FSLILEESYEHKYPYDWRTK
Sbjct: 486 LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDHFSLILEESYEHKYPYDWRTK 545
Query: 545 KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQRTWGV 604
KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRIS MTSSRSDWCISRQRTWGV
Sbjct: 546 KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISAMTSSRSDWCISRQRTWGV 605
Query: 605 PIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYEKGTD 664
PIPVFYHV+TKEPLMNDDT+NHIKSIIAKKGSDAWWYM V+DLLPDKYREIASEYEKGTD
Sbjct: 606 PIPVFYHVQTKEPLMNDDTVNHIKSIIAKKGSDAWWYMTVEDLLPDKYREIASEYEKGTD 665
Query: 665 TMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI 724
TMDVWFDSGSSWAAVLGT+DGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI
Sbjct: 666 TMDVWFDSGSSWAAVLGTRDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI 725
Query: 725 THGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGDVMIG 784
THGFVLDEKGLKMSKSLGNVVDPK+VIEGGKNQKE PGYGADVLRLWVSSVDYTGDVMIG
Sbjct: 726 THGFVLDEKGLKMSKSLGNVVDPKSVIEGGKNQKEAPGYGADVLRLWVSSVDYTGDVMIG 785
Query: 785 SQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINNIRES 844
SQVLRQMSDIYRKLRGTLRFLLGNLHDWTE+N VQYQDLP+IDQHALFQLEN+INNIRES
Sbjct: 786 SQVLRQMSDIYRKLRGTLRFLLGNLHDWTEENAVQYQDLPRIDQHALFQLENIINNIRES 845
Query: 845 YESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVSITRI 904
YESYQF KIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLA H+VSI RI
Sbjct: 846 YESYQFFKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLAAHIVSIARI 905
Query: 905 IAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWANILEL 964
IAPILPHLAEDVWQNLPF++ NDDGS AKFVFESRWPSLNKTRL LPREEVDFWANILEL
Sbjct: 906 IAPILPHLAEDVWQNLPFQYTNDDGSAAKFVFESRWPSLNKTRLSLPREEVDFWANILEL 965
Query: 965 RTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFITSQA 1024
RTEVNKVLEVART KLIGSSLEAKVHLH SGDGL +KL EMCESNH+ADTLQRIFITSQ
Sbjct: 966 RTEVNKVLEVARTGKLIGSSLEAKVHLHASGDGLATKLCEMCESNHDADTLQRIFITSQV 1025
Query: 1025 EVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHPTLCK 1084
EVHR+IENEL EN PY GECLI GSKVWIGVSRAEGSKCERCWNFSLRVGSF EHPTLCK
Sbjct: 1026 EVHRAIENELTENTPYAGECLIEGSKVWIGVSRAEGSKCERCWNFSLRVGSFPEHPTLCK 1085
Query: 1085 RCFDVVAGHPEPAMAAIS 1101
RCFDVVAG+PEPAMAA+S
Sbjct: 1086 RCFDVVAGYPEPAMAAVS 1098
BLAST of MC05g0625 vs. NCBI nr
Match:
XP_023527875.1 (isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2045 bits (5298), Expect = 0.0
Identity = 1013/1099 (92.17%), Postives = 1042/1099 (94.81%), Query Frame = 0
Query: 5 MPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASL-RRSSISSFSFRGSSSAKLF 64
MP YAS A SSA KCK ATKSLI +S Y VLSQRISASL RRS I SF FRGSSSAK+F
Sbjct: 6 MPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISASLGRRSYIPSFRFRGSSSAKVF 65
Query: 65 SFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFGMRA 124
+FL VT YSTYLNDEF SSKRR+RGPVMAA+KASKET EGDGKYKHTVDLPKTTFGMRA
Sbjct: 66 TFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRA 125
Query: 125 NSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR 184
NS+VREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR
Sbjct: 126 NSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR 185
Query: 185 YKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMI 244
YKLLQNYKVRFVPGWDCHGLPI+ VLQSLDKEARKDLTPIKLRDKAAKFAKATVK QM
Sbjct: 186 YKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKTQMA 245
Query: 245 SFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTA 304
SFK RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTA
Sbjct: 246 SFK-----RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTA 305
Query: 305 LAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANAAVA 364
LAEAELEYPEGHTSRSIYAIFRLVKEGP S G+LQEFLPDLCLAIWTTTPWTIPANAAVA
Sbjct: 306 LAEAELEYPEGHTSRSIYAIFRLVKEGPSSGGMLQEFLPDLCLAIWTTTPWTIPANAAVA 365
Query: 365 VNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWGMKL 424
VNAKL YAIVEL APSEDVS SGNKK+RPGNIL EE +PFLVVASDLVPTLEAKWG+KL
Sbjct: 366 VNAKLHYAIVELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 425
Query: 425 VVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTGMKY 484
VVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDY TG+KY
Sbjct: 426 VVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYATGLKY 485
Query: 485 GLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYDWRT 544
GLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDD FSLILEESYEHKYPYDWRT
Sbjct: 486 GLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDHFSLILEESYEHKYPYDWRT 545
Query: 545 KKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQRTWG 604
KKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRIS MTSSRSDWCISRQRTWG
Sbjct: 546 KKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISAMTSSRSDWCISRQRTWG 605
Query: 605 VPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYEKGT 664
VPIPVFYHV+TKEPLMNDDT+NHIKSIIAKKGSDAWWYM V+DLLPDKYREIASEYEKGT
Sbjct: 606 VPIPVFYHVQTKEPLMNDDTVNHIKSIIAKKGSDAWWYMTVEDLLPDKYREIASEYEKGT 665
Query: 665 DTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSV 724
DTMDVWFDSGSSWAAVLGT+DGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSV
Sbjct: 666 DTMDVWFDSGSSWAAVLGTRDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSV 725
Query: 725 ITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGDVMI 784
ITHGFVLDEKGLKMSKSLGNVVDPK+VIEGGKNQKE PGYGADVLRLWVSSVDYTGDVMI
Sbjct: 726 ITHGFVLDEKGLKMSKSLGNVVDPKSVIEGGKNQKEAPGYGADVLRLWVSSVDYTGDVMI 785
Query: 785 GSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINNIRE 844
GSQVLRQMSDIYRKLRGTLRFLLGNLHDWTE+N VQYQDLP+IDQHALFQLEN+INNIRE
Sbjct: 786 GSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEENAVQYQDLPRIDQHALFQLENIINNIRE 845
Query: 845 SYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVSITR 904
SYESYQF KIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLA H+VSI R
Sbjct: 846 SYESYQFFKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLAAHIVSIAR 905
Query: 905 IIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWANILE 964
IIAPILPHLAEDVWQNLPF++ NDDGS AKFVFESRWPSLNKTRL LPREEVDFWANILE
Sbjct: 906 IIAPILPHLAEDVWQNLPFQYTNDDGSAAKFVFESRWPSLNKTRLSLPREEVDFWANILE 965
Query: 965 LRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFITSQ 1024
LRTEVNKVLEVART KLIGSSLEAKVHLH SGDGL +KL EMCESNH+ADTLQRIFITSQ
Sbjct: 966 LRTEVNKVLEVARTGKLIGSSLEAKVHLHASGDGLATKLCEMCESNHDADTLQRIFITSQ 1025
Query: 1025 AEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHPTLC 1084
EVHR+IENEL ENIPYTGECLI G KVWIGVSRAEGSKCERCWNFSLRVGSF EHP LC
Sbjct: 1026 VEVHRAIENELTENIPYTGECLIEGIKVWIGVSRAEGSKCERCWNFSLRVGSFPEHPALC 1085
Query: 1085 KRCFDVVAGHPEPAMAAIS 1101
KRCFDV+AG+PEPAMAA+S
Sbjct: 1086 KRCFDVIAGYPEPAMAAVS 1099
BLAST of MC05g0625 vs. NCBI nr
Match:
XP_008438885.1 (PREDICTED: isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo])
HSP 1 Score: 2044 bits (5296), Expect = 0.0
Identity = 1008/1102 (91.47%), Postives = 1045/1102 (94.83%), Query Frame = 0
Query: 1 MEATMPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSA 60
MEA MP YAS A SSAFKCKEATK I + Y VLSQRIS++LRRS ISSF FRGSSSA
Sbjct: 1 MEANMPLYASFAAGSSAFKCKEATKPFIQTPSYTVLSQRISSTLRRSYISSFRFRGSSSA 60
Query: 61 KLFSFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFG 120
K+F+FL VT YSTYLNDEFP SSKRRSRGP+MAA+KAS+ET EGDGKYKHTVDLPKTTFG
Sbjct: 61 KVFTFLTVTRYSTYLNDEFPPSSKRRSRGPIMAAKKASQETNEGDGKYKHTVDLPKTTFG 120
Query: 121 MRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 180
MRANS VREPEIQKIWDDHQVFNR VERNTGENFILHDGPPYANGDLHMGHALNKILKDI
Sbjct: 121 MRANSLVREPEIQKIWDDHQVFNRAVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 180
Query: 181 INRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 240
INRYKLLQNYKVRFVPGWDCHGLPI+ VLQSLDKEARKDLTPIKLRDKAAKFAKATVKA
Sbjct: 181 INRYKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 240
Query: 241 QMISFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 300
QM SFK RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS
Sbjct: 241 QMASFK-----RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 300
Query: 301 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANA 360
RTALAEAELEYPEGHTSRSIYAIFRLVKEGP S G+LQEFLPDLCLAIWTTTPWTIPANA
Sbjct: 301 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPSSGGVLQEFLPDLCLAIWTTTPWTIPANA 360
Query: 361 AVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWG 420
AVAVNAKL YAI+EL APSED S+ +GNKK++PGNILKE K+PFLVVASDLVPTLEAKWG
Sbjct: 361 AVAVNAKLHYAIIELLAPSEDASVLTGNKKKKPGNILKEGKRPFLVVASDLVPTLEAKWG 420
Query: 421 MKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTG 480
+K+VVKKKLLGSELENC YIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDY TG
Sbjct: 421 VKIVVKKKLLGSELENCSYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYATG 480
Query: 481 MKYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYD 540
+KYGLP+ISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDD FSLILEESYEHKYPYD
Sbjct: 481 LKYGLPIISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDHFSLILEESYEHKYPYD 540
Query: 541 WRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQR 600
WRTKKPTIFRATEQWFASVEGFRQAA+DAIGEVKWIPPQAENRIS MTSSRSDWCISRQR
Sbjct: 541 WRTKKPTIFRATEQWFASVEGFRQAAVDAIGEVKWIPPQAENRISAMTSSRSDWCISRQR 600
Query: 601 TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYE 660
TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYM VDDLLPDKYREIASEYE
Sbjct: 601 TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMAVDDLLPDKYREIASEYE 660
Query: 661 KGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPY 720
KGTDTMDVWFDSGSSWAAVLGT+DGFSFPADLYLEGTDQHRGWFQSSLLT IATKGKAPY
Sbjct: 661 KGTDTMDVWFDSGSSWAAVLGTRDGFSFPADLYLEGTDQHRGWFQSSLLTCIATKGKAPY 720
Query: 721 SSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGD 780
+SV+THGFVLDEKGLKMSKSLGNVVDPK VIEGGKNQKETPGYGADVLRLWVSSVDYTGD
Sbjct: 721 ASVVTHGFVLDEKGLKMSKSLGNVVDPKAVIEGGKNQKETPGYGADVLRLWVSSVDYTGD 780
Query: 781 VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINN 840
VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTE+N VQYQDLPKIDQHALFQLENVINN
Sbjct: 781 VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEENAVQYQDLPKIDQHALFQLENVINN 840
Query: 841 IRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVS 900
IRESYESYQF KIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLA H+VS
Sbjct: 841 IRESYESYQFFKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLAAHVVS 900
Query: 901 ITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWAN 960
I RIIAPILPHLAEDVWQNLPF+H NDDGSVAKFVFESRWPSLNKTRL LP+EE+DFWAN
Sbjct: 901 IARIIAPILPHLAEDVWQNLPFQHTNDDGSVAKFVFESRWPSLNKTRLSLPKEEIDFWAN 960
Query: 961 ILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFI 1020
ILELRTEVNKVLE ART KLIGSSLEAKVHLHT DGL SKL EMCESNH+ADTLQRIFI
Sbjct: 961 ILELRTEVNKVLEAARTGKLIGSSLEAKVHLHTP-DGLSSKLCEMCESNHDADTLQRIFI 1020
Query: 1021 TSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHP 1080
TSQ EVH+S+ENE +EN+PYTGECLI GSKVWIGVSRAEGSKCERCWNFS+RVGSF EHP
Sbjct: 1021 TSQVEVHQSVENEHIENVPYTGECLIGGSKVWIGVSRAEGSKCERCWNFSIRVGSFPEHP 1080
Query: 1081 TLCKRCFDVVAGHPEPAMAAIS 1101
TLCKRCF+VVAGHPEPAMAA+S
Sbjct: 1081 TLCKRCFNVVAGHPEPAMAAVS 1096
BLAST of MC05g0625 vs. ExPASy TrEMBL
Match:
A0A6J1CBR1 (Isoleucyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111009237 PE=3 SV=1)
HSP 1 Score: 2137 bits (5536), Expect = 0.0
Identity = 1062/1102 (96.37%), Postives = 1072/1102 (97.28%), Query Frame = 0
Query: 1 MEATMPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSA 60
MEATMPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSA
Sbjct: 1 MEATMPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSA 60
Query: 61 KLFSFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFG 120
KLFSFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFG
Sbjct: 61 KLFSFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFG 120
Query: 121 MRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 180
MRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDI
Sbjct: 121 MRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 180
Query: 181 INRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 240
INRYKLLQNYKVRFVPGWDCHGLPI+ VLQSLDKEARKDLTPIKLRDKAAKFAKATVKA
Sbjct: 181 INRYKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 240
Query: 241 QMISFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 300
QMISFK RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS
Sbjct: 241 QMISFK-----RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 300
Query: 301 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANA 360
RTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANA
Sbjct: 301 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANA 360
Query: 361 AVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWG 420
AVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWG
Sbjct: 361 AVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWG 420
Query: 421 MKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTG 480
MKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTG
Sbjct: 421 MKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTG 480
Query: 481 MKYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYD 540
MKYGLPLISPVDDDGKFTEE + L + ++++ +F ++ EHKYPYD
Sbjct: 481 MKYGLPLISPVDDDGKFTEEGEKMIILIPTRFLSFLFLRFISVRFQYVISSYTEHKYPYD 540
Query: 541 WRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQR 600
WRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQR
Sbjct: 541 WRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQR 600
Query: 601 TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYE 660
TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYE
Sbjct: 601 TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYE 660
Query: 661 KGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPY 720
KGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPY
Sbjct: 661 KGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPY 720
Query: 721 SSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGD 780
SSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGD
Sbjct: 721 SSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGD 780
Query: 781 VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINN 840
VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINN
Sbjct: 781 VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINN 840
Query: 841 IRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVS 900
IRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVS
Sbjct: 841 IRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVS 900
Query: 901 ITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWAN 960
ITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL LPREEVDFWAN
Sbjct: 901 ITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRLSLPREEVDFWAN 960
Query: 961 ILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFI 1020
ILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFI
Sbjct: 961 ILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFI 1020
Query: 1021 TSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHP 1080
TSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHP
Sbjct: 1021 TSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHP 1080
Query: 1081 TLCKRCFDVVAGHPEPAMAAIS 1101
TLCKRCFDVVAGHPEPAMAAIS
Sbjct: 1081 TLCKRCFDVVAGHPEPAMAAIS 1097
BLAST of MC05g0625 vs. ExPASy TrEMBL
Match:
A0A6J1GYE1 (Isoleucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457972 PE=3 SV=1)
HSP 1 Score: 2050 bits (5310), Expect = 0.0
Identity = 1014/1098 (92.35%), Postives = 1043/1098 (94.99%), Query Frame = 0
Query: 5 MPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSAKLFS 64
MP YAS A SSA KCK ATKSLI +S Y VLSQRISASL RS I SF FRGSSSAK+F+
Sbjct: 6 MPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISASLGRSYIPSFRFRGSSSAKVFT 65
Query: 65 FLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFGMRAN 124
FL VT YSTYLNDEF SSKRR+RGPVMAA+KASKET EGDGKYKHTVDLPKTTFGMRAN
Sbjct: 66 FLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRAN 125
Query: 125 SSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRY 184
S+VREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRY
Sbjct: 126 STVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRY 185
Query: 185 KLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMIS 244
KLLQNYKVRFVPGWDCHGLPI+ VLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQM S
Sbjct: 186 KLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMAS 245
Query: 245 FKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL 304
FK RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL
Sbjct: 246 FK-----RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL 305
Query: 305 AEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANAAVAV 364
AEAELEYPEGHTSRSIYAIFRLVKEGP S G+LQEFLPDLCLAIWTTTPWTIPANAAVAV
Sbjct: 306 AEAELEYPEGHTSRSIYAIFRLVKEGPSSGGMLQEFLPDLCLAIWTTTPWTIPANAAVAV 365
Query: 365 NAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWGMKLV 424
NAKL YAIVEL APSEDVS SGNKK+RPGNIL EE +PFLVVASDLVPTLEAKWG+KLV
Sbjct: 366 NAKLHYAIVELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKLV 425
Query: 425 VKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTGMKYG 484
VKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDY TG+KYG
Sbjct: 426 VKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYATGLKYG 485
Query: 485 LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYDWRTK 544
LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDD FSLILEESYEHKYPYDWRTK
Sbjct: 486 LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDHFSLILEESYEHKYPYDWRTK 545
Query: 545 KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQRTWGV 604
KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRIS MTSSRSDWCISRQRTWGV
Sbjct: 546 KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISAMTSSRSDWCISRQRTWGV 605
Query: 605 PIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYEKGTD 664
PIPVFYHV+TKEPLMNDDT+NHIKSIIAKKGSDAWWYM V+DLLPDKYREIASEYEKGTD
Sbjct: 606 PIPVFYHVQTKEPLMNDDTVNHIKSIIAKKGSDAWWYMTVEDLLPDKYREIASEYEKGTD 665
Query: 665 TMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI 724
TMDVWFDSGSSWAAVLGT+DGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI
Sbjct: 666 TMDVWFDSGSSWAAVLGTRDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI 725
Query: 725 THGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGDVMIG 784
THGFVLDEKGLKMSKSLGNVVDPK+VIEGGKNQKE PGYGADVLRLWVSSVDYTGDVMIG
Sbjct: 726 THGFVLDEKGLKMSKSLGNVVDPKSVIEGGKNQKEAPGYGADVLRLWVSSVDYTGDVMIG 785
Query: 785 SQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINNIRES 844
SQVLRQMSDIYRKLRGTLRFLLGNLHDWTE+N VQYQDLP+IDQHALFQLEN+INNIRES
Sbjct: 786 SQVLRQMSDIYRKLRGTLRFLLGNLHDWTEENAVQYQDLPRIDQHALFQLENIINNIRES 845
Query: 845 YESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVSITRI 904
YESYQF KIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLA H+VSI RI
Sbjct: 846 YESYQFFKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLAAHIVSIARI 905
Query: 905 IAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWANILEL 964
IAPILPHLAEDVWQNLPF++ NDDGS AKFVFESRWPSLNKTRL LPREEVDFWANILEL
Sbjct: 906 IAPILPHLAEDVWQNLPFQYTNDDGSAAKFVFESRWPSLNKTRLSLPREEVDFWANILEL 965
Query: 965 RTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFITSQA 1024
RTEVNKVLEVART KLIGSSLEAKVHLH SGDGL +KL EMCESNH+ADTLQRIFITSQ
Sbjct: 966 RTEVNKVLEVARTGKLIGSSLEAKVHLHASGDGLATKLCEMCESNHDADTLQRIFITSQV 1025
Query: 1025 EVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHPTLCK 1084
EVH++IENEL EN PYTGECLI GSKVWIGVSRAEGSKCERCWNFSLRVGSF EHPTLCK
Sbjct: 1026 EVHQAIENELTENTPYTGECLIEGSKVWIGVSRAEGSKCERCWNFSLRVGSFPEHPTLCK 1085
Query: 1085 RCFDVVAGHPEPAMAAIS 1101
RCFDVVAG+PEPAMAA+S
Sbjct: 1086 RCFDVVAGYPEPAMAAVS 1098
BLAST of MC05g0625 vs. ExPASy TrEMBL
Match:
A0A6J1IX89 (Isoleucyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479329 PE=3 SV=1)
HSP 1 Score: 2044 bits (5296), Expect = 0.0
Identity = 1009/1098 (91.89%), Postives = 1041/1098 (94.81%), Query Frame = 0
Query: 5 MPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSAKLFS 64
MP YAS A SSA KCK TKSL+ +S Y VLSQRISASL RS I SF FRGSSSAK+F+
Sbjct: 6 MPLYASFAAGSSALKCKGVTKSLVQTSSYSVLSQRISASLGRSYIPSFRFRGSSSAKVFT 65
Query: 65 FLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFGMRAN 124
FL VTHYSTYLNDEF SSKRR+RGPVMAA+KASKET EGDGKYKHTVDLPKTTFGMRAN
Sbjct: 66 FLSVTHYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRAN 125
Query: 125 SSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRY 184
S+VREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHA+NKILKDIINRY
Sbjct: 126 STVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHAMNKILKDIINRY 185
Query: 185 KLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMIS 244
KLLQNYKVRFVPGWDCHGLPI+ VLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQM S
Sbjct: 186 KLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMAS 245
Query: 245 FKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL 304
FK RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL
Sbjct: 246 FK-----RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTAL 305
Query: 305 AEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANAAVAV 364
AEAELEYPEGHTSRSIYAIFRLVKEGP S G+LQEFLPDLCLAIWTTTPWTIPANAAVAV
Sbjct: 306 AEAELEYPEGHTSRSIYAIFRLVKEGPSSGGMLQEFLPDLCLAIWTTTPWTIPANAAVAV 365
Query: 365 NAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWGMKLV 424
NAKL YAIVEL APSEDVS SGNKK+RPGNIL EE +PFLVVASDLVPTLEAKWG+KLV
Sbjct: 366 NAKLHYAIVELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKLV 425
Query: 425 VKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTGMKYG 484
VK+KLLGSELENCRYIHPI+KRECQVVIGGDYITTETGTGLVHTAPGHGQEDY TG+KYG
Sbjct: 426 VKEKLLGSELENCRYIHPINKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYATGLKYG 485
Query: 485 LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYDWRTK 544
LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDD FSLILEE YEHKYPYDWRTK
Sbjct: 486 LPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDHFSLILEELYEHKYPYDWRTK 545
Query: 545 KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQRTWGV 604
KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRIS MTSSRSDWCISRQRTWGV
Sbjct: 546 KPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISAMTSSRSDWCISRQRTWGV 605
Query: 605 PIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYEKGTD 664
PIPVFYHV+TKEPLMNDDT+NHIKSIIAKKGSDAWWYM V+DLLPDKYREIASEYEKGTD
Sbjct: 606 PIPVFYHVQTKEPLMNDDTVNHIKSIIAKKGSDAWWYMTVEDLLPDKYREIASEYEKGTD 665
Query: 665 TMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI 724
TMDVWFDSGSSWAAVLGT+DGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI
Sbjct: 666 TMDVWFDSGSSWAAVLGTRDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVI 725
Query: 725 THGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGDVMIG 784
THGFVLDEKGLKMSKSLGNVVDPK+VIEGGKNQKE PGYGADVLRLWVSSVDYTGDVMIG
Sbjct: 726 THGFVLDEKGLKMSKSLGNVVDPKSVIEGGKNQKEAPGYGADVLRLWVSSVDYTGDVMIG 785
Query: 785 SQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINNIRES 844
SQVLRQMSDIYRKLRGTLRFLLGNLHDWTE+N VQYQDLP+IDQHALFQLEN+INNIRES
Sbjct: 786 SQVLRQMSDIYRKLRGTLRFLLGNLHDWTEENAVQYQDLPRIDQHALFQLENIINNIRES 845
Query: 845 YESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVSITRI 904
YESYQF KIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLA H+VSI RI
Sbjct: 846 YESYQFFKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLAAHIVSIARI 905
Query: 905 IAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWANILEL 964
IAPILPHLAEDVWQNLPF++ NDDGS AKFVFESRWPSLNKTRL LPREEVDFWANILEL
Sbjct: 906 IAPILPHLAEDVWQNLPFQYTNDDGSAAKFVFESRWPSLNKTRLSLPREEVDFWANILEL 965
Query: 965 RTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFITSQA 1024
RTEVNKVLEVART KLIGSSLEAKVHLH SGDGL +KL EMCESNH+ADTLQRIFITSQ
Sbjct: 966 RTEVNKVLEVARTGKLIGSSLEAKVHLHVSGDGLATKLCEMCESNHDADTLQRIFITSQV 1025
Query: 1025 EVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHPTLCK 1084
EVHR+IENEL EN PYTGECLI GS+VWIGVSRAEGSKCERCWNFSLRVGSF EHPTLCK
Sbjct: 1026 EVHRAIENELTENTPYTGECLIEGSQVWIGVSRAEGSKCERCWNFSLRVGSFPEHPTLCK 1085
Query: 1085 RCFDVVAGHPEPAMAAIS 1101
RCFDVVA HPEPAMAA+S
Sbjct: 1086 RCFDVVASHPEPAMAAVS 1098
BLAST of MC05g0625 vs. ExPASy TrEMBL
Match:
A0A1S3AY52 (Isoleucyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103483844 PE=3 SV=1)
HSP 1 Score: 2044 bits (5296), Expect = 0.0
Identity = 1008/1102 (91.47%), Postives = 1045/1102 (94.83%), Query Frame = 0
Query: 1 MEATMPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSA 60
MEA MP YAS A SSAFKCKEATK I + Y VLSQRIS++LRRS ISSF FRGSSSA
Sbjct: 1 MEANMPLYASFAAGSSAFKCKEATKPFIQTPSYTVLSQRISSTLRRSYISSFRFRGSSSA 60
Query: 61 KLFSFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFG 120
K+F+FL VT YSTYLNDEFP SSKRRSRGP+MAA+KAS+ET EGDGKYKHTVDLPKTTFG
Sbjct: 61 KVFTFLTVTRYSTYLNDEFPPSSKRRSRGPIMAAKKASQETNEGDGKYKHTVDLPKTTFG 120
Query: 121 MRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 180
MRANS VREPEIQKIWDDHQVFNR VERNTGENFILHDGPPYANGDLHMGHALNKILKDI
Sbjct: 121 MRANSLVREPEIQKIWDDHQVFNRAVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 180
Query: 181 INRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 240
INRYKLLQNYKVRFVPGWDCHGLPI+ VLQSLDKEARKDLTPIKLRDKAAKFAKATVKA
Sbjct: 181 INRYKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 240
Query: 241 QMISFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 300
QM SFK RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS
Sbjct: 241 QMASFK-----RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 300
Query: 301 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANA 360
RTALAEAELEYPEGHTSRSIYAIFRLVKEGP S G+LQEFLPDLCLAIWTTTPWTIPANA
Sbjct: 301 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPSSGGVLQEFLPDLCLAIWTTTPWTIPANA 360
Query: 361 AVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWG 420
AVAVNAKL YAI+EL APSED S+ +GNKK++PGNILKE K+PFLVVASDLVPTLEAKWG
Sbjct: 361 AVAVNAKLHYAIIELLAPSEDASVLTGNKKKKPGNILKEGKRPFLVVASDLVPTLEAKWG 420
Query: 421 MKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTG 480
+K+VVKKKLLGSELENC YIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDY TG
Sbjct: 421 VKIVVKKKLLGSELENCSYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYATG 480
Query: 481 MKYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYD 540
+KYGLP+ISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDD FSLILEESYEHKYPYD
Sbjct: 481 LKYGLPIISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDHFSLILEESYEHKYPYD 540
Query: 541 WRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQR 600
WRTKKPTIFRATEQWFASVEGFRQAA+DAIGEVKWIPPQAENRIS MTSSRSDWCISRQR
Sbjct: 541 WRTKKPTIFRATEQWFASVEGFRQAAVDAIGEVKWIPPQAENRISAMTSSRSDWCISRQR 600
Query: 601 TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYE 660
TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYM VDDLLPDKYREIASEYE
Sbjct: 601 TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMAVDDLLPDKYREIASEYE 660
Query: 661 KGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPY 720
KGTDTMDVWFDSGSSWAAVLGT+DGFSFPADLYLEGTDQHRGWFQSSLLT IATKGKAPY
Sbjct: 661 KGTDTMDVWFDSGSSWAAVLGTRDGFSFPADLYLEGTDQHRGWFQSSLLTCIATKGKAPY 720
Query: 721 SSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGD 780
+SV+THGFVLDEKGLKMSKSLGNVVDPK VIEGGKNQKETPGYGADVLRLWVSSVDYTGD
Sbjct: 721 ASVVTHGFVLDEKGLKMSKSLGNVVDPKAVIEGGKNQKETPGYGADVLRLWVSSVDYTGD 780
Query: 781 VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINN 840
VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTE+N VQYQDLPKIDQHALFQLENVINN
Sbjct: 781 VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEENAVQYQDLPKIDQHALFQLENVINN 840
Query: 841 IRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVS 900
IRESYESYQF KIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLA H+VS
Sbjct: 841 IRESYESYQFFKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLAAHVVS 900
Query: 901 ITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWAN 960
I RIIAPILPHLAEDVWQNLPF+H NDDGSVAKFVFESRWPSLNKTRL LP+EE+DFWAN
Sbjct: 901 IARIIAPILPHLAEDVWQNLPFQHTNDDGSVAKFVFESRWPSLNKTRLSLPKEEIDFWAN 960
Query: 961 ILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFI 1020
ILELRTEVNKVLE ART KLIGSSLEAKVHLHT DGL SKL EMCESNH+ADTLQRIFI
Sbjct: 961 ILELRTEVNKVLEAARTGKLIGSSLEAKVHLHTP-DGLSSKLCEMCESNHDADTLQRIFI 1020
Query: 1021 TSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHP 1080
TSQ EVH+S+ENE +EN+PYTGECLI GSKVWIGVSRAEGSKCERCWNFS+RVGSF EHP
Sbjct: 1021 TSQVEVHQSVENEHIENVPYTGECLIGGSKVWIGVSRAEGSKCERCWNFSIRVGSFPEHP 1080
Query: 1081 TLCKRCFDVVAGHPEPAMAAIS 1101
TLCKRCF+VVAGHPEPAMAA+S
Sbjct: 1081 TLCKRCFNVVAGHPEPAMAAVS 1096
BLAST of MC05g0625 vs. ExPASy TrEMBL
Match:
A0A5A7U5N1 (Isoleucyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G007500 PE=3 SV=1)
HSP 1 Score: 2028 bits (5254), Expect = 0.0
Identity = 1004/1111 (90.37%), Postives = 1041/1111 (93.70%), Query Frame = 0
Query: 1 MEATMPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSSISSFSFRGSSSA 60
MEA MP YAS A SSAFKCKEATK I + Y VLSQRIS++LRRS ISSF FRGSSSA
Sbjct: 1 MEANMPLYASFAAGSSAFKCKEATKPFIQTPSYTVLSQRISSTLRRSYISSFRFRGSSSA 60
Query: 61 KLFSFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFG 120
K+F+FL VT YSTYLNDEFP SSKRRSRGP+MAA+KAS+ET EGDGKYKHT+DLPKTTFG
Sbjct: 61 KVFTFLTVTRYSTYLNDEFPPSSKRRSRGPIMAAKKASQETNEGDGKYKHTIDLPKTTFG 120
Query: 121 MRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 180
MRANS VREPEIQKIWDDHQVFNR VERNTGENFILHDGPPYANGDLHMGHALNKILKDI
Sbjct: 121 MRANSLVREPEIQKIWDDHQVFNRAVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 180
Query: 181 INRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 240
INRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA
Sbjct: 181 INRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 240
Query: 241 QMISFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 300
QM SFK FL RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS
Sbjct: 241 QMASFKANFL-RYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 300
Query: 301 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANA 360
RTALAEAELEYPEGHTSRSIYAIFRLVKEGP S G+LQEFLPDLCLAIWTTTPWTIPANA
Sbjct: 301 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPSSGGVLQEFLPDLCLAIWTTTPWTIPANA 360
Query: 361 AVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWG 420
AVAVNAKL YAI+EL APSED S+ +GNKK++PGNILKE K+PFLVVASDLVPTLEAKW
Sbjct: 361 AVAVNAKLHYAIIELLAPSEDASVLTGNKKKKPGNILKEGKRPFLVVASDLVPTLEAKWD 420
Query: 421 MKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTG 480
+K+VVKKKLLGSELENC YIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDY TG
Sbjct: 421 VKIVVKKKLLGSELENCSYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYATG 480
Query: 481 MKYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESY------- 540
+KYGLP+ISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDD FSL +
Sbjct: 481 LKYGLPIISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDHFSLGSPSLFGSNMSSL 540
Query: 541 --EHKYPYDWRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSR 600
EHKYPYDWRTKKPTIFRATEQWFASVEGFRQAA+DAIGEVKWIPPQAENRIS MTSSR
Sbjct: 541 LTEHKYPYDWRTKKPTIFRATEQWFASVEGFRQAAVDAIGEVKWIPPQAENRISAMTSSR 600
Query: 601 SDWCISRQRTWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDK 660
SDWCISRQRTWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYM VDDLLPDK
Sbjct: 601 SDWCISRQRTWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMAVDDLLPDK 660
Query: 661 YREIASEYEKGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTS 720
YREIASEYEKGTDTMDVWFDSGSSWAAVLGT+DGFSFPADLYLEGTDQHRGWFQSSLLT
Sbjct: 661 YREIASEYEKGTDTMDVWFDSGSSWAAVLGTRDGFSFPADLYLEGTDQHRGWFQSSLLTC 720
Query: 721 IATKGKAPYSSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLW 780
IATKGKAPY+SV+THGFVLDEKGLKMSKSLGNVVDPK VIEGGKNQKE PGYGADVLRLW
Sbjct: 721 IATKGKAPYASVVTHGFVLDEKGLKMSKSLGNVVDPKAVIEGGKNQKEAPGYGADVLRLW 780
Query: 781 VSSVDYTGDVMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHAL 840
VSSVDYTGDVMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTE+N VQYQDLPKIDQHAL
Sbjct: 781 VSSVDYTGDVMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEENAVQYQDLPKIDQHAL 840
Query: 841 FQLENVINNIRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQ 900
FQLENVINNIRESYESYQF KIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQ
Sbjct: 841 FQLENVINNIRESYESYQFFKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQ 900
Query: 901 TVLADHLVSITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LP 960
TVLA H+VSI RIIAPILPHLAEDVWQNLPF+H NDDGSVAKFVFESRWPSLNK RL LP
Sbjct: 901 TVLAAHVVSIARIIAPILPHLAEDVWQNLPFQHTNDDGSVAKFVFESRWPSLNKIRLSLP 960
Query: 961 REEVDFWANILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHE 1020
+EE+DFWANILELRTEVNKVLE ART KLIGSSLEAKVHLHT DGL SKL EMCESNH+
Sbjct: 961 KEEIDFWANILELRTEVNKVLEAARTGKLIGSSLEAKVHLHTP-DGLSSKLCEMCESNHD 1020
Query: 1021 ADTLQRIFITSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSL 1080
ADTLQRIFITSQ EVH+S+ENE +EN+PYTGECLI GSKVWIGVSRAEGSKCERCWNFSL
Sbjct: 1021 ADTLQRIFITSQVEVHQSVENEHIENVPYTGECLIGGSKVWIGVSRAEGSKCERCWNFSL 1080
Query: 1081 RVGSFLEHPTLCKRCFDVVAGHPEPAMAAIS 1101
RVGSF EHPTLCKRCF+VVAGHPEPAMAA+S
Sbjct: 1081 RVGSFPEHPTLCKRCFNVVAGHPEPAMAAVS 1109
BLAST of MC05g0625 vs. TAIR 10
Match:
AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )
HSP 1 Score: 1685.6 bits (4364), Expect = 0.0e+00
Identity = 809/1082 (74.77%), Postives = 939/1082 (86.78%), Query Frame = 0
Query: 23 ATKSLICSSPYRVLSQRISASLRRSS-ISSFSFRGSSSAKLFSFLRVTHYSTYLNDEFPF 82
A +++ SS YRVLS + ++LRR++ + SF +G SS K FSFL V+ +ST N+EF
Sbjct: 16 AAMAMVQSSSYRVLSGKSCSNLRRNTPLDSFLAKGRSSVKAFSFLYVSRFSTEPNNEFGH 75
Query: 83 SSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFGMRANSSVREPEIQKIWDDHQV 142
SSKRRSRGPVMAA+KAS+ + DGKYK TVDLPKT FGMRANS REPE+QK+W+++QV
Sbjct: 76 SSKRRSRGPVMAAKKASEGEKQEDGKYKQTVDLPKTGFGMRANSLTREPELQKLWEENQV 135
Query: 143 FNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVRFVPGWDCH 202
F RV + N G +FILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKV++VPGWDCH
Sbjct: 136 FKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWDCH 195
Query: 203 GLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMISFKVQFLQRYGVWADWNN 262
GLPI+ VLQSLD+E RK+LTP+KLR KAAKFAKATVK QM SFK R+GVWADWNN
Sbjct: 196 GLPIELKVLQSLDQEVRKELTPLKLRAKAAKFAKATVKTQMESFK-----RFGVWADWNN 255
Query: 263 PYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIY 322
PYLTL PEYEAAQIEVFGQMA++GYI+RGRKPVHWSPSSRTALAEAELEYPEGH S+SIY
Sbjct: 256 PYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIY 315
Query: 323 AIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLQYAIVELRAPSED 382
AIF+LV G LL EF+P++ LA+WTTTPWT+PANAAVAVNAKLQY++VE+++ SED
Sbjct: 316 AIFKLV--GGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSFSED 375
Query: 383 VSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWGMKLVVKKKLLGSELENCRYIH 442
S + NKK+ PG +LK ++K F++VA+DLVP LEAKWG+KL + K LGS+LENCRY H
Sbjct: 376 ESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCRYTH 435
Query: 443 PIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTGMKYGLPLISPVDDDGKFTEEA 502
PID R+C VVIGGDYITTE+GTGLVHTAPGHGQEDY TG+KYGLPL+SPVDD+GKFTEEA
Sbjct: 436 PIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEA 495
Query: 503 GQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYDWRTKKPTIFRATEQWFASVEG 562
GQF GL VLG+GNTAVV YLD+ SL++EESY HKYPYDWRTKKPTIFRATEQWFASVEG
Sbjct: 496 GQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEG 555
Query: 563 FRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQRTWGVPIPVFYHVETKEPLMND 622
FR A MDAI VKW+P QA NRIS MTSSRSDWCISRQRTWGVPIP FYHV+TKEPLMN+
Sbjct: 556 FRTATMDAINNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNE 615
Query: 623 DTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYEKGTDTMDVWFDSGSSWAAVLG 682
+TINH+KSII++KGSDAWWYM V+DLLP+KYR+ A++YEKGTDTMDVWFDSGSSWA VLG
Sbjct: 616 ETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDVWFDSGSSWAGVLG 675
Query: 683 TQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVITHGFVLDEKGLKMSKSL 742
++G SFPAD+YLEGTDQHRGWFQSSLLTSIAT+GKAPYS+VITHGFVLDEKG+KMSKSL
Sbjct: 676 KREGLSFPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSL 735
Query: 743 GNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGDVMIGSQVLRQMSDIYRKLRGT 802
GNVVDP+ VIEGGKN K+ P YGADV+RLWVSSVDYTGDV+IG Q+LRQMSDIYRKLRGT
Sbjct: 736 GNVVDPRLVIEGGKNSKDAPAYGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRGT 795
Query: 803 LRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINNIRESYESYQFCKIFQIIQRFV 862
LR+LLGNLHDW DN V YQDLP IDQHALFQLENV+ NI+E YE+YQF KIFQIIQRF
Sbjct: 796 LRYLLGNLHDWRVDNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFT 855
Query: 863 IVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVSITRIIAPILPHLAEDVWQNLP 922
IVDLSNFYFD+AKDRLY GG++SFTRRSCQTVL+ HL+SI R+IAPI+PHLAEDVWQNLP
Sbjct: 856 IVDLSNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLP 915
Query: 923 FEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWANILELRTEVNKVLEVARTEKLI 982
FE++N+DGS A+FVFE +WP+LN+ L P E+V FW +LELRTEVNKVLE+AR EK+I
Sbjct: 916 FEYRNEDGSAAEFVFELKWPTLNEQWLSFPAEDVLFWQRLLELRTEVNKVLELARNEKMI 975
Query: 983 GSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFITSQAEVHRSIENELVENIPYT 1042
GSSLEAKV+LHT+ G+ +KL EM E+ +EADTLQRIFITSQ EV S+E E++ ++ +T
Sbjct: 976 GSSLEAKVYLHTADAGMAAKLLEMSEAKNEADTLQRIFITSQVEVLSSMEKEMISSVQHT 1035
Query: 1043 GECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHPTLCKRCFDV-VAGHPEPAMAA 1102
GE + +KVWIGVSRAEGSKCERCWN+S +VGSF +HPTLC RCF V VA PEPA+AA
Sbjct: 1036 GEYVEGENKVWIGVSRAEGSKCERCWNYSGQVGSFSDHPTLCGRCFSVIVANPPEPAVAA 1090
BLAST of MC05g0625 vs. TAIR 10
Match:
AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )
HSP 1 Score: 1684.1 bits (4360), Expect = 0.0e+00
Identity = 813/1103 (73.71%), Postives = 947/1103 (85.86%), Query Frame = 0
Query: 2 EATMPQYASSATSSSAFKCKEATKSLICSSPYRVLSQRISASLRRSS-ISSFSFRGSSSA 61
E + Q +S S + + A +++ SS YRVLS + ++LRR++ + SF +G SS
Sbjct: 181 ERNLKQMSSFFKSFAGNPREAAAMAMVQSSSYRVLSGKSCSNLRRNTPLDSFLAKGRSSV 240
Query: 62 KLFSFLRVTHYSTYLNDEFPFSSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFG 121
K FSFL V+ +ST N+EF SSKRRSRGPVMAA+KAS+ + DGKYK TVDLPKT FG
Sbjct: 241 KAFSFLYVSRFSTEPNNEFGHSSKRRSRGPVMAAKKASEGEKQEDGKYKQTVDLPKTGFG 300
Query: 122 MRANSSVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDI 181
MRANS REPE+QK+W+++QVF RV + N G +FILHDGPPYANGDLHMGHALNKILKDI
Sbjct: 301 MRANSLTREPELQKLWEENQVFKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDI 360
Query: 182 INRYKLLQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKA 241
INRYKLLQNYKV++VPGWDCHGLPI+ VLQSLD+E RK+LTP+KLR KAAKFAKATVK
Sbjct: 361 INRYKLLQNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPLKLRAKAAKFAKATVKT 420
Query: 242 QMISFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSS 301
QM SFK R+GVWADWNNPYLTL PEYEAAQIEVFGQMA++GYI+RGRKPVHWSPSS
Sbjct: 421 QMESFK-----RFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSS 480
Query: 302 RTALAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANA 361
RTALAEAELEYPEGH S+SIYAIF+LV G LL EF+P++ LA+WTTTPWT+PANA
Sbjct: 481 RTALAEAELEYPEGHISKSIYAIFKLV--GGAKTSLLDEFIPNIYLAVWTTTPWTMPANA 540
Query: 362 AVAVNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWG 421
AVAVNAKLQY++VE+++ SED S + NKK+ PG +LK ++K F++VA+DLVP LEAKWG
Sbjct: 541 AVAVNAKLQYSVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWG 600
Query: 422 MKLVVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTG 481
+KL + K LGS+LENCRY HPID R+C VVIGGDYITTE+GTGLVHTAPGHGQEDY TG
Sbjct: 601 VKLSISKTFLGSDLENCRYTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATG 660
Query: 482 MKYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYD 541
+KYGLPL+SPVDD+GKFTEEAGQF GL VLG+GNTAVV YLD+ SL++EESY HKYPYD
Sbjct: 661 LKYGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYD 720
Query: 542 WRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQR 601
WRTKKPTIFRATEQWFASVEGFR A MDAI VKW+P QA NRIS MTSSRSDWCISRQR
Sbjct: 721 WRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVPHQAVNRISAMTSSRSDWCISRQR 780
Query: 602 TWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYE 661
TWGVPIP FYHV+TKEPLMN++TINH+KSII++KGSDAWWYM V+DLLP+KYR+ A++YE
Sbjct: 781 TWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAADYE 840
Query: 662 KGTDTMDVWFDSGSSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPY 721
KGTDTMDVWFDSGSSWA VLG ++G SFPAD+YLEGTDQHRGWFQSSLLTSIAT+GKAPY
Sbjct: 841 KGTDTMDVWFDSGSSWAGVLGKREGLSFPADVYLEGTDQHRGWFQSSLLTSIATQGKAPY 900
Query: 722 SSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGD 781
S+VITHGFVLDEKG+KMSKSLGNVVDP+ VIEGGKN K+ P YGADV+RLWVSSVDYTGD
Sbjct: 901 SAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWVSSVDYTGD 960
Query: 782 VMIGSQVLRQMSDIYRKLRGTLRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINN 841
V+IG Q+LRQMSDIYRKLRGTLR+LLGNLHDW DN V YQDLP IDQHALFQLENV+ N
Sbjct: 961 VLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAVPYQDLPIIDQHALFQLENVVKN 1020
Query: 842 IRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVS 901
I+E YE+YQF KIFQIIQRF IVDLSNFYFD+AKDRLY GG++SFTRRSCQTVL+ HL+S
Sbjct: 1021 IQECYENYQFFKIFQIIQRFTIVDLSNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLS 1080
Query: 902 ITRIIAPILPHLAEDVWQNLPFEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWAN 961
I R+IAPI+PHLAEDVWQNLPFE++N+DGS A+FVFE +WP+LN+ L P E+V FW
Sbjct: 1081 ILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFELKWPTLNEQWLSFPAEDVLFWQR 1140
Query: 962 ILELRTEVNKVLEVARTEKLIGSSLEAKVHLHTSGDGLVSKLSEMCESNHEADTLQRIFI 1021
+LELRTEVNKVLE+AR EK+IGSSLEAKV+LHT+ G+ +KL EM E+ +EADTLQRIFI
Sbjct: 1141 LLELRTEVNKVLELARNEKMIGSSLEAKVYLHTADAGMAAKLLEMSEAKNEADTLQRIFI 1200
Query: 1022 TSQAEVHRSIENELVENIPYTGECLIRGSKVWIGVSRAEGSKCERCWNFSLRVGSFLEHP 1081
TSQ EV S+E E++ ++ +TGE + +KVWIGVSRAEGSKCERCWN+S +VGSF +HP
Sbjct: 1201 TSQVEVLSSMEKEMISSVQHTGEYVEGENKVWIGVSRAEGSKCERCWNYSGQVGSFSDHP 1260
Query: 1082 TLCKRCFDV-VAGHPEPAMAAIS 1102
TLC RCF V VA PEPA+AA++
Sbjct: 1261 TLCGRCFSVIVANPPEPAVAAVN 1276
BLAST of MC05g0625 vs. TAIR 10
Match:
AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )
HSP 1 Score: 1500.3 bits (3883), Expect = 0.0e+00
Identity = 715/943 (75.82%), Postives = 824/943 (87.38%), Query Frame = 0
Query: 23 ATKSLICSSPYRVLSQRISASLRRSS-ISSFSFRGSSSAKLFSFLRVTHYSTYLNDEFPF 82
A +++ SS YRVLS + ++LRR++ + SF +G SS K FSFL V+ +ST N+EF
Sbjct: 16 AAMAMVQSSSYRVLSGKSCSNLRRNTPLDSFLAKGRSSVKAFSFLYVSRFSTEPNNEFGH 75
Query: 83 SSKRRSRGPVMAARKASKETIEGDGKYKHTVDLPKTTFGMRANSSVREPEIQKIWDDHQV 142
SSKRRSRGPVMAA+KAS+ + DGKYK TVDLPKT FGMRANS REPE+QK+W+++QV
Sbjct: 76 SSKRRSRGPVMAAKKASEGEKQEDGKYKQTVDLPKTGFGMRANSLTREPELQKLWEENQV 135
Query: 143 FNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVRFVPGWDCH 202
F RV + N G +FILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKV++VPGWDCH
Sbjct: 136 FKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWDCH 195
Query: 203 GLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMISFKVQFLQRYGVWADWNN 262
GLPI+ VLQSLD+E RK+LTP+KLR KAAKFAKATVK QM SFK R+GVWADWNN
Sbjct: 196 GLPIELKVLQSLDQEVRKELTPLKLRAKAAKFAKATVKTQMESFK-----RFGVWADWNN 255
Query: 263 PYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIY 322
PYLTL PEYEAAQIEVFGQMA++GYI+RGRKPVHWSPSSRTALAEAELEYPEGH S+SIY
Sbjct: 256 PYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIY 315
Query: 323 AIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLQYAIVELRAPSED 382
AIF+LV G LL EF+P++ LA+WTTTPWT+PANAAVAVNAKLQY++VE+++ SED
Sbjct: 316 AIFKLV--GGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSFSED 375
Query: 383 VSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWGMKLVVKKKLLGSELENCRYIH 442
S + NKK+ PG +LK ++K F++VA+DLVP LEAKWG+KL + K LGS+LENCRY H
Sbjct: 376 ESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCRYTH 435
Query: 443 PIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTGMKYGLPLISPVDDDGKFTEEA 502
PID R+C VVIGGDYITTE+GTGLVHTAPGHGQEDY TG+KYGLPL+SPVDD+GKFTEEA
Sbjct: 436 PIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEA 495
Query: 503 GQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYDWRTKKPTIFRATEQWFASVEG 562
GQF GL VLG+GNTAVV YLD+ SL++EESY HKYPYDWRTKKPTIFRATEQWFASVEG
Sbjct: 496 GQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEG 555
Query: 563 FRQAAMDAIGEVKWIPPQAENRISVMTSSRSDWCISRQRTWGVPIPVFYHVETKEPLMND 622
FR A MDAI VKW+P QA NRIS MTSSRSDWCISRQRTWGVPIP FYHV+TKEPLMN+
Sbjct: 556 FRTATMDAINNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNE 615
Query: 623 DTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYEKGTDTMDVWFDSGSSWAAVLG 682
+TINH+KSII++KGSDAWWYM V+DLLP+KYR+ A++YEKGTDTMDVWFDSGSSWA VLG
Sbjct: 616 ETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDVWFDSGSSWAGVLG 675
Query: 683 TQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATKGKAPYSSVITHGFVLDEKGLKMSKSL 742
++G SFPAD+YLEGTDQHRGWFQSSLLTSIAT+GKAPYS+VITHGFVLDEKG+KMSKSL
Sbjct: 676 KREGLSFPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSL 735
Query: 743 GNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSVDYTGDVMIGSQVLRQMSDIYRKLRGT 802
GNVVDP+ VIEGGKN K+ P YGADV+RLWVSSVDYTGDV+IG Q+LRQMSDIYRKLRGT
Sbjct: 736 GNVVDPRLVIEGGKNSKDAPAYGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRGT 795
Query: 803 LRFLLGNLHDWTEDNYVQYQDLPKIDQHALFQLENVINNIRESYESYQFCKIFQIIQRFV 862
LR+LLGNLHDW DN V YQDLP IDQHALFQLENV+ NI+E YE+YQF KIFQIIQRF
Sbjct: 796 LRYLLGNLHDWRVDNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFT 855
Query: 863 IVDLSNFYFDVAKDRLYVGGSTSFTRRSCQTVLADHLVSITRIIAPILPHLAEDVWQNLP 922
IVDLSNFYFD+AKDRLY GG++SFTRRSCQTVL+ HL+SI R+IAPI+PHLAEDVWQNLP
Sbjct: 856 IVDLSNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLP 915
Query: 923 FEHKNDDGSVAKFVFESRWPSLNKTRL-LPREEVDFWANILEL 964
FE++N+DGS A+FVFE +WP+LN+ L P E+V FW +LE+
Sbjct: 916 FEYRNEDGSAAEFVFELKWPTLNEQWLSFPAEDVLFWQRLLEV 951
BLAST of MC05g0625 vs. TAIR 10
Match:
AT4G10320.1 (tRNA synthetase class I (I, L, M and V) family protein )
HSP 1 Score: 305.4 bits (781), Expect = 1.8e-82
Identity = 232/846 (27.42%), Postives = 387/846 (45.74%), Query Frame = 0
Query: 128 REPEIQKIWDDHQVFNRVVERNTG-ENFILHDGPPYANGDLHMGHALNKILKDIINRYKL 187
+E ++ W + F ++R +I +DGPP+A G H GH L +KDI+ RY+
Sbjct: 15 QEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQT 74
Query: 188 LQNYKVRFVPGWDCHGLPIDYSVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMISFK 247
+ + V GWDCHGLP++ + + L+ + R ++ + + DK + ++ V + ++
Sbjct: 75 MTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGI-DKYNEECRSIVTRYVAEWE 134
Query: 248 VQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALA- 307
+ + R G W D+ N Y T+ + + VF Q+ + ++RG K + +S +T L+
Sbjct: 135 -KVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVMPYSTGCKTPLSN 194
Query: 308 -EAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANAAVAV 367
EA Y E I F ++ + + WTTTPWT+P+N A+ V
Sbjct: 195 FEAGQNYKE-VPDPEIMVTFPVIGD-----------QDNAAFVAWTTTPWTLPSNLALCV 254
Query: 368 NAKLQYAIVE--------LRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLE 427
NAK Y V + A S +L + K + N + K A P
Sbjct: 255 NAKFVYVKVRNKNTGKVYIVAESRLSALPTDKPKAKLSNGPAGDTKKANPKAKGAKPESA 314
Query: 428 AKWGMKLVVKKKLLGSELENCRYIHPID-----KRECQVVIGGDYITTETGTGLVHTAPG 487
A V +K G+ L +Y D E V+ DY+T ++GTG+VH AP
Sbjct: 315 AD---SYEVLEKFNGASLVGKKYEPLFDYFSDFSSEAFRVVADDYVTDDSGTGIVHCAPA 374
Query: 488 HGQEDYVTGM-----KYGLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFS 547
G++DY + K G L+ VDDDG FTE FSG V D + +++ + +
Sbjct: 375 FGEDDYRVCLLNKIIKKGENLVVAVDDDGLFTERITHFSGRYV-KDADKDIIEAVKAKGR 434
Query: 548 LILEESYEHKYPYDWRTKKPTIFRATEQWFASVEGFRQAAMDAIGEVKWIPPQA-ENRIS 607
L+ S+ H YP+ WR+ P I+RA WF VE ++ + + + +W+P + R
Sbjct: 435 LVKTGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEQLKEKLLKSNEQTEWVPGYVKDKRFH 494
Query: 608 VMTSSRSDWCISRQRTWGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVD 667
+ DW ISR R WG P+P++ + +E ++ D++ ++ + K D +
Sbjct: 495 NWLENARDWAISRSRFWGTPLPIWISDDGEEVVIM-DSVEKLEKLSGVKVFDLHRHHIDH 554
Query: 668 DLLPDKYREIASEYEKGTDTMDVWFDSGSSWAAVL----GTQDGF--SFPADLYLEGTDQ 727
+P + + D D WF+SGS A + ++ F +FP D EG DQ
Sbjct: 555 ITIPSSRGDEFGVLRRVEDVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDFVAEGLDQ 614
Query: 728 HRGWFQSSLLTSIATKGKAPYSSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKE 787
RGWF + ++ S A K + ++I +G VL E G KM+K L N P VI+
Sbjct: 615 TRGWFYTLMVLSTALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVID------- 674
Query: 788 TPGYGADVLRLWV--SSVDYTGDVMIGSQ-VLRQMSDIYRKLRGTLRFLLGNLHDWTEDN 847
YGAD +RL++ S V + + VL + D++ RFL+ N +
Sbjct: 675 --EYGADAVRLYLINSPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEG 734
Query: 848 YVQY--------QDLPKIDQHALFQLENVINNIRESYESYQFCKIFQIIQRFVIVDLSNF 907
V + Q +DQ ++++ +RE ++Y+ + + +F + +L+N
Sbjct: 735 GVPFVPTDLATIQSANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKF-LDNLTNI 794
Query: 908 YFDVAKDRLYVGGSTSFTRRSCQTVLA---DHLVSITRIIAPILPHLAEDVWQNLPFEHK 932
Y + RL G T C T L+ + L++ +++AP P E ++QNL K
Sbjct: 795 YVRFNRKRL--KGRTG--EDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACK 827
BLAST of MC05g0625 vs. TAIR 10
Match:
AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )
HSP 1 Score: 228.8 bits (582), Expect = 2.1e-59
Identity = 207/819 (25.27%), Postives = 337/819 (41.15%), Query Frame = 0
Query: 129 EPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQ 188
E +I K W+ F ++ G F++ PP G LHMGHA+ L+DI+ RY +
Sbjct: 78 EEKIYKWWESQGYFKPNFDQG-GSPFVIPMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMN 137
Query: 189 NYKVRFVPGWDCHGLPIDYSVLQSLDKEA--RKDLTPIKLRDKAAKFAK---ATVKAQMI 248
++PG D G+ V + L E R DL + + ++ + T+ Q
Sbjct: 138 GRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVDLGRDEFTKRVWEWKEKYGGTITNQ-- 197
Query: 249 SFKVQFLQRYGVWADWNNPYLTLSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTA 308
++R G DW+ TL + A +E F ++ +G I++G V+WSP+ +TA
Sbjct: 198 ------IKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMVNWSPNLQTA 257
Query: 309 LAEAELEYPEGHTSRSIYAIFRLVKEGPPSCGLLQEFLPDLCLAIWTTTPWTIPANAAVA 368
+++ E+EY E +Y I V P L I TT P T+ + A+A
Sbjct: 258 VSDLEVEYSE--EPGFLYHIKYRVAGSPD------------FLTIATTRPETLFGDVALA 317
Query: 369 VNAKLQYAIVELRAPSEDVSLSSGNKKQRPGNILKEEKKPFLVVASDLVPTLEAKWGMKL 428
V+ P +D
Sbjct: 318 VH------------PEDD------------------------------------------ 377
Query: 429 VVKKKLLGSELENCRYIHPIDKRECQVVIGGDYITTETGTGLVHTAPGHGQEDYVTGMKY 488
K +G I P+ +I Y+ + GTG++ +PGH DY+ K
Sbjct: 378 -RYSKYVGQTA-----IVPMTYGRHVPIIADKYVDKDFGTGVLKISPGHDHNDYLLARKL 437
Query: 489 GLPLISPVDDDGKFTEEAGQFSGLDVLGDGNTAVVKYLDDQFSLILEESYEHKYPYDWRT 548
GLP+++ ++ D + AG F GLD + + L++ + +E + + P R
Sbjct: 438 GLPILNVMNKDATLNDVAGLFCGLDRF-EVREKLWADLEEIGLAVKKEPHTLRVPRSQRG 497
Query: 549 KKPTIFRATEQWFASVEGFRQAAMDAI--GEVKWIPPQAENRISVMTSSRSDWCISRQRT 608
+ ++QWF ++ + A+ A+ E+ IP + E + ++ DWCISRQ
Sbjct: 498 GEVIEPLVSKQWFVHMDPLAEKALLAVENKELTIIPERFEKIYNHWLTNIKDWCISRQLW 557
Query: 609 WGVPIPVFYHVETKEPLMNDDTINHIKSIIAKKGSDAWWYMKVDDLLPDKYREIASEYEK 668
WG IPV+Y V +D I+AK +A + +KY + Y+
Sbjct: 558 WGHRIPVWYVVGKD---CEED------YIVAKSAEEAL------EKALEKYGKDVEIYQ- 617
Query: 669 GTDTMDVWFDSG------SSWAAVLGTQDGFSFPADLYLEGTDQHRGWFQSSLLTSIATK 728
D +D WF S W V +P ++ G D W ++ I
Sbjct: 618 DPDVLDTWFSSSLWPFSTLGWPDVAAKDFNNFYPTNMLETGHDILFFWVARMVMMGIEFT 677
Query: 729 GKAPYSSVITHGFVLDEKGLKMSKSLGNVVDPKTVIEGGKNQKETPGYGADVLRLWVSSV 788
G P+S V HG + D +G KMSKSLGNV+DP I+ +G D LR ++
Sbjct: 678 GTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTIK---------DFGTDALRFTIALG 737
Query: 789 DYTGDVMIGSQVLRQMSDIYRKLRGTLRFLL------------GNLHDWTEDNYVQYQDL 848
D+ + ++ L KL +F+L NL D D L
Sbjct: 738 TAGQDLNLSTERLTANKAFTNKLWNAGKFVLHSLPSLSDTSAWENLLDLKLDKEETLLSL 786
Query: 849 PKIDQHALFQLENVINNIRESYESYQFCKIFQIIQRFVIVDLSNFYFDVAKDRLYVGGST 908
P + A+ +L +I+++ SYE F + + F D +++Y + +K RLY G
Sbjct: 798 PLPECWAVSKLHILIDSVTASYEKLFFGDVGRETYDFFWSDFADWYIEASKSRLYGSGGN 786
Query: 909 SFTRRSCQTVLADHLVSITRIIAPILPHLAEDVWQNLPF 923
S + S Q VL +I +++ P +P + ED+WQ LP+
Sbjct: 858 SVSLAS-QAVLLYVFENILKLLHPFMPFVTEDLWQALPY 786
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q8RXK8 | 0.0e+00 | 74.77 | Isoleucine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... | [more] |
| Q8YXY3 | 0.0e+00 | 57.63 | Isoleucine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... | [more] |
| B7K5H5 | 0.0e+00 | 57.03 | Isoleucine--tRNA ligase OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=ile... | [more] |
| B7KFT1 | 0.0e+00 | 56.21 | Isoleucine--tRNA ligase OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=... | [more] |
| B2IZP5 | 0.0e+00 | 56.64 | Isoleucine--tRNA ligase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022137948.1 | 0.0 | 96.37 | isoleucine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] | [more] |
| XP_022956209.1 | 0.0 | 92.35 | isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata] | [more] |
| KAG6582211.1 | 0.0 | 92.26 | Isoleucine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyros... | [more] |
| XP_023527875.1 | 0.0 | 92.17 | isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo... | [more] |
| XP_008438885.1 | 0.0 | 91.47 | PREDICTED: isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CBR1 | 0.0 | 96.37 | Isoleucyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111009237 PE=3 SV... | [more] |
| A0A6J1GYE1 | 0.0 | 92.35 | Isoleucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457972 PE=3 SV=... | [more] |
| A0A6J1IX89 | 0.0 | 91.89 | Isoleucyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479329 PE=3 SV=1 | [more] |
| A0A1S3AY52 | 0.0 | 91.47 | Isoleucyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103483844 PE=3 SV=1 | [more] |
| A0A5A7U5N1 | 0.0 | 90.37 | Isoleucyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT5G49030.1 | 0.0e+00 | 74.77 | tRNA synthetase class I (I, L, M and V) family protein | [more] |
| AT5G49030.3 | 0.0e+00 | 73.71 | tRNA synthetase class I (I, L, M and V) family protein | [more] |
| AT5G49030.2 | 0.0e+00 | 75.82 | tRNA synthetase class I (I, L, M and V) family protein | [more] |
| AT4G10320.1 | 1.8e-82 | 27.42 | tRNA synthetase class I (I, L, M and V) family protein | [more] |
| AT5G16715.1 | 2.1e-59 | 25.27 | ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi... | [more] |
