MC05g0549 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0549
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	ATP-dependent (S)-NAD(P)H-hydrate dehydratase
Location	MC05: 4182681 .. 4187483 (+)
RNA-Seq Expression	MC05g0549
Synteny	MC05g0549

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCGTCTTTCTTCTCCTCCTCCTCCCGTCGAAAGGCGAAGAACTCTTAAGGTATGCTGAACCTGAAATTTTTCTATCCGTTATTGAATTTGGACTCTCATTAGCTTGGCTGATTCTCTAAGAAGAATAGTTCTTCCCCTAAATCGGAGCTGAATTAGGTTGCATTTTTTTCCCCTAATAATTGCTTTGATTTTGGTTTCTATGACGAAGATTGAGGCTCCTGGCTCAAATTGAGCTTTGATTTTGGTTTCAATTGTAAAATTCTTCTGTGGGTTAAAGAACCGAATTATTTTAAGTCTCATTTCTGTTGCATTAGTAGAAGGTACTGATTTTCCACAGAATGAAGTAACTATTTTCGGATTCCATCACGTTCGAGACCTAATCAGTTGACGTGAATCGACAGTGCAAAGTAATTGAATTTTGGCTTCCTCGGCTGTGTTTAGACGACAACAGTTCTTGATAAGGTCTCTTGGAGGTTTCGGTGATTGCACCCATCAGAATAGGCGGCAACAAATCAAAGCCATGAGTGGGACTTCTGTTGAGGCTGATGCTGAGAACATTATAAGAGCAATTACACCTTCTCTTGATCCCAACAGATACAAAGGCCAGGCGGGTAAGCTCAAAATGTACCCTGTAGTTCTATGGCTTTTCTTTTATTTTCCATCTGCGCAAATGGTTGGTAAATACTCGAGTATATTTTTTGTGATGAATCAGTTTGCAAGAACTTTATAGTTAGTCAACTTTTCAAATTGATGGTTTTTGGCTATTGGATAGATTTTGCTAACTCATTTTCAATATTGATCCCTGGTGTCAGTTTACGCTTTTGTCTTCCAATTACGTTTTATGCTTTATTTTATGATTTTCCCTGTGTTAGAATTAGAGTGTGGCTAAGGTTGAGAAATACTCTATTTAACAATCTATATATGTTTATCAGGGAAGATAGCTGTTATTGGTGGATGTCGTGAGTACACAGGTGCTCCATACTTCGCTGCTATATCAGCTTTGAAAATTGTTAGTTCAGACTCTTTTTCTTGGTTGTCTTGTTAGAGAATTTTATTCTACATTTTTGTTTATATAATTAATTTTTCCTCTTTTTACTCGTGAAGGTAAGGTGTATAACAAACAAACCATTTGTTTTTCCTTTCAGGGTGCAGATTTGTCTCACGTTTTCTGCACAAAAGATGCTGCTCCTGTCATAAAGAGCTATAGTCCAGAGTTAATTGTGCATCCCGTTTTAGAAGAATCATACAGCGTCAGGTGCTTTTTGCTTTCTATATTTCGGTCCGAGGGAAAAAAAAGAATTAAATGGAAATTTTAATATTTTTCTAATGCCTCTAATGATGTTTTAGAGATGAGGAAAAGAAGTTCATTGCAGAAAAGGTTCTTGCTGAGGTTGACAAGTGGCTGGAAAGATTTGATTGCCTTGTCATTGGTCCAGGCCTCGGAAGGGACCCATTTCTTCTGGTGATTTTGTTATTGCTTTTTGGCTAAATTATATAATTTGCTCTTGAACTATGGACTTTATTTTAAAAGCTTTAATGATATCTTCAAGTTTTCAACCTTTTGGCTGACATGTTTGAGAAAATGAGATTTTTCATATTGATTTGGCAAATGAGATAGTGAAAGCCCTCTCCTTTTTTCCTTCTTCATGCTAACTGCTGAATGAATGAAGGTAAAATCCTTCTTTACTGTAGTCCTTTTGCCACATTGGGACGAAAACCTCATTTTTGTTGTACACGTCAGTCAGTAGTTTTAAAAATAACCATTCATGTCCATTTCTCATTGTTTTTTAGTGGTTGGGAGTCTTAATTTAAATCAATCAAAAGTATGGGGGTAGGTAGTTGAGAATTTGAAAACTTAAGGAACAAATTTGAAATCAAGTGCATAGTTAGGGAAGGATGCTAGAACTTTTCCCATGTCTTAATTCTTTCGAGTAGATTGAACTAAGATGCATAGTTCGATAACGATTGTATTTTCTTATCTGAAAATGATTGTCAAGTCATATTGTTACCCAAACTTGATATGCATAAGGTACTCCCAGATGCCTTGCAAACAGAACTTCAGCAGCGTCTCTGACTATTACTTGTAATTATTGAGCTGCCCCTGCTTCAAATGATAGACTTTGAAAGTGCAGATTAGTTGGTTCTATTATAGGCTAAGGACAATGCCTCAAGATCATTAGAACTAGTAAGTCTGATGATAATTTGTTTCAAATAATCATTTTGGACAAGCTGATTAGGGCTATATATGCTAACTTGGCGTAAATTCTACAAATGGTTTCAATACCAGGGGCTGGTAACTTGATACCCCTACAATTATTTGTTCAAAGGATTTTAGGTGCGAAAATCAAATCAATTGAGATCCCAAAGATAGATACAAAAGATGTAAGAAATTCCACTCTCTGAGTCTTTACAAAAAATGGAAAAATTTTGATTCTCTGTGTCTCTCTTTTGCCTTTTCAGACCACTAAAATGGTAAAATGAAGTTAATTACTCCACTGTTTCTTCAATTACATTGGTCTGATATTTTCTGGCGGTTGCTTTGGCTGTAAGCCTATGCCTTTTTACTTTTTCAACAAGTCTTCAAACATGAATTATAATCATCCCAGAGGTTGAAGTGATTTTTCAAATCTAACAACTTTCTCCTTTGGATTTGTCATCAAATTTCTTTGTCTTGCCTATGAAACGCTACCATAAAATTTTGCTAATGGTTGAAAACTGTTGCAGGATTGTGTCAGCGAAATCATAAAGCATGCAAGGCAGACCAATATACCAATGGTTATAGACGGGGTATGCATATACCAAAGCAAATACGATTTTGCCTTGTTTATACTATTGTTATGCATGCTACTATCCAACAGGTCTCTAGGAGGTGGCTGCTGACAGCTGGTTGTGTTTGTTGCTTTTCAGGATGGACTCTTTCTTATCACTGACAATCTTCACCTTGTAAGCAATTACCCCCTGGCCGTCCTAACTCCAAATGTAAACGAATATAAACGTCTTGTGAAAAATGTGCTGCTTGCTGAAATCGATGAACAAGATGCTTCTAAGCAATTGCTGTCCCTCGTCAAAAGGTAAAGTCTTCATGTTTTCCCATCTGAATAGAGTTTCCATATGTCATGATTCTGGAATGGTGACGTACTTATTGCTCAAGTGAATCTTGTATGGACACACTACCTAGCGCAACCCCAAAATCAGCGGGTTAAGGTACATATATTTACAAAAGTTGGAAGCTGAGGGGTTGACCTCATCTGATCAGAATTCAGAATAGATGTTACTACATATTCAGGACTCATTATCGTTTTTTCTAAATGAAGTAACTCTATTTGCACATTCTGAAGTCTTTAGCCAAATGGTTCAATGTCAAGAATGGGAATGTTTTGGTGGTGTGTCCTACCCTTCCAACAGTTGATCACTCCTCGATTTAGCCTACTTCTTTCCTGAAATAATTATTTATGAAGTATGAAGGTACTAACATTAGGATGAAAATTATAACTGTATCTTACTAAAGGGATTCTTGTTGGCTAGCTATAAATAATCATCTGGCATTCTATATTGCCAAAGATAATTGGAGATAGCTTTTCCTACGCGGGTTTATGCTCCATTCTATTTTATAATTTCTTACATAGTGTACATTATTATTTAATTTAATTTTCCAGTCTTTGGTTCTCTAAACTTTTGGACATGATCAGGATTGGTGGCCTAACTATACTCAGGAAAGGGAGAGCTGACCTCATCAGTGACGGTGAGACAGGTACCGAGTACAACTCCAAAATAATTTTCCATGTCAATGTTGTAAAAGTGCTACATTCAATGTTTCTTCTCTATGATTAGGAGATTAATTGATTGTCAGTGTCACCAACCAGAAGTTTACCTCCTGGTCAGATTAGGAAAAAAACAATAAAATTCCCTCTTATGTATACTAAGCTAAGCTGTTGTCCTGATATCATGGTGGAAGTGAATCAAATTGAATATAGATTGAAAGTGTTCTTTCTAATTTCTGTAAAATCCTTTGGATTTTTGGTTTTGACCTGTCATAATGTCCATGAAGATTTAGGAATTTGTGCTTCTTAAGGGTTGTTGTAAATACTAATACGGTGTCAGAGTTTCTCCCTTGCTAGATTATACTGGATTCTTCCATTAAGTGAATTTTATGGTACCCGATGCAGTACAATCAGTGAGCATCTATGGTTCTCCTCGCCGTTGTGGTGGCCAAGGTGATATACTTTCAGGAAGGCAAGTACTGCCATGAATTAATCTTTTCAACGTACCTTATTTATATTTCTCATTCAAGAGTTGAGAACCCTAAAAGTTCACGACTTTCTTCAACAATGTCTGAAATTGTTTTCTTTCTCCCTGTGGTTTCAGTGTTGCAGTTTTCGTATCTTGGGCACGGCGGCAAGGCTTAGCCACAGATGGGAATTTGGCTACCAGGCAAGTTTTAGAATTTAAACAGCATATGATTTTCAATCTCATCTGACGGATTCTGATTCCATATATGATCTTTTATGAAGCCCTTTTACACATAGCCCAAAGAATCCAACCATATTAGGGTGCATTGCTGGATCTGCTCTACTTAGAAGGGCTGCATCACTTGCATTTGAGAATAAGAAAAGATCAACCCTTACTACCGACATCATCGAGTTCTTGTGGAGAAGGTACGGTGAACGTCGGGGATGGAGATCATTTATATATAAAAAACAGATCAACTTCTAATATTGGTTCCTTCTTGCAGTCTGGAGGATATTTCTCCGGCTCACTGATCAAATCTGCTGATCTTGCAACCTTGCTGGAGGTACTCATG

mRNA sequence

CTTCGTCTTTCTTCTCCTCCTCCTCCCGTCGAAAGGCGAAGAACTCTTAAGACGACAACAGTTCTTGATAAGGTCTCTTGGAGGTTTCGGTGATTGCACCCATCAGAATAGGCGGCAACAAATCAAAGCCATGAGTGGGACTTCTGTTGAGGCTGATGCTGAGAACATTATAAGAGCAATTACACCTTCTCTTGATCCCAACAGATACAAAGGCCAGGCGGGGAAGATAGCTGTTATTGGTGGATGTCGTGAGTACACAGGTGCTCCATACTTCGCTGCTATATCAGCTTTGAAAATTGGTGCAGATTTGTCTCACGTTTTCTGCACAAAAGATGCTGCTCCTGTCATAAAGAGCTATAGTCCAGAGTTAATTGTGCATCCCGTTTTAGAAGAATCATACAGCGTCAGAGATGAGGAAAAGAAGTTCATTGCAGAAAAGGTTCTTGCTGAGGTTGACAAGTGGCTGGAAAGATTTGATTGCCTTGTCATTGGTCCAGGCCTCGGAAGGGACCCATTTCTTCTGGATTGTGTCAGCGAAATCATAAAGCATGCAAGGCAGACCAATATACCAATGGTTATAGACGGGGATGGACTCTTTCTTATCACTGACAATCTTCACCTTGTAAGCAATTACCCCCTGGCCGTCCTAACTCCAAATGTAAACGAATATAAACGTCTTGTGAAAAATGTGCTGCTTGCTGAAATCGATGAACAAGATGCTTCTAAGCAATTGCTGTCCCTCGTCAAAAGGATTGGTGGCCTAACTATACTCAGGAAAGGGAGAGCTGACCTCATCAGTGACGGTGAGACAGTACAATCAGTGAGCATCTATGGTTCTCCTCGCCGTTGTGGTGGCCAAGGTGATATACTTTCAGGAAGTGTTGCAGTTTTCGTATCTTGGGCACGGCGGCAAGGCTTAGCCACAGATGGGAATTTGGCTACCAGCCCAAAGAATCCAACCATATTAGGGTGCATTGCTGGATCTGCTCTACTTAGAAGGGCTGCATCACTTGCATTTGAGAATAAGAAAAGATCAACCCTTACTACCGACATCATCGAGTTCTTGTGGAGAAGATCAACTTCTAATATTGGTTCCTTCTTGCAGTCTGGAGGATATTTCTCCGGCTCACTGATCAAATCTGCTGATCTTGCAACCTTGCTGGAGGTACTCATG

Coding sequence (CDS)

TTCGTCTTTCTTCTCCTCCTCCTCCCGTCGAAAGGCGAAGAACTCTTAAGACGACAACAGTTCTTGATAAGGTCTCTTGGAGGTTTCGGTGATTGCACCCATCAGAATAGGCGGCAACAAATCAAAGCCATGAGTGGGACTTCTGTTGAGGCTGATGCTGAGAACATTATAAGAGCAATTACACCTTCTCTTGATCCCAACAGATACAAAGGCCAGGCGGGGAAGATAGCTGTTATTGGTGGATGTCGTGAGTACACAGGTGCTCCATACTTCGCTGCTATATCAGCTTTGAAAATTGGTGCAGATTTGTCTCACGTTTTCTGCACAAAAGATGCTGCTCCTGTCATAAAGAGCTATAGTCCAGAGTTAATTGTGCATCCCGTTTTAGAAGAATCATACAGCGTCAGAGATGAGGAAAAGAAGTTCATTGCAGAAAAGGTTCTTGCTGAGGTTGACAAGTGGCTGGAAAGATTTGATTGCCTTGTCATTGGTCCAGGCCTCGGAAGGGACCCATTTCTTCTGGATTGTGTCAGCGAAATCATAAAGCATGCAAGGCAGACCAATATACCAATGGTTATAGACGGGGATGGACTCTTTCTTATCACTGACAATCTTCACCTTGTAAGCAATTACCCCCTGGCCGTCCTAACTCCAAATGTAAACGAATATAAACGTCTTGTGAAAAATGTGCTGCTTGCTGAAATCGATGAACAAGATGCTTCTAAGCAATTGCTGTCCCTCGTCAAAAGGATTGGTGGCCTAACTATACTCAGGAAAGGGAGAGCTGACCTCATCAGTGACGGTGAGACAGTACAATCAGTGAGCATCTATGGTTCTCCTCGCCGTTGTGGTGGCCAAGGTGATATACTTTCAGGAAGTGTTGCAGTTTTCGTATCTTGGGCACGGCGGCAAGGCTTAGCCACAGATGGGAATTTGGCTACCAGCCCAAAGAATCCAACCATATTAGGGTGCATTGCTGGATCTGCTCTACTTAGAAGGGCTGCATCACTTGCATTTGAGAATAAGAAAAGATCAACCCTTACTACCGACATCATCGAGTTCTTGTGGAGAAGATCAACTTCTAATATTGGTTCCTTCTTGCAGTCTGGAGGATATTTCTCCGGCTCACTGATCAAATCTGCTGATCTTGCAACCTTGCTGGAGGTACTCATG

Protein sequence

FVFLLLLLPSKGEELLRRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSPKNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWRRSTSNIGSFLQSGGYFSGSLIKSADLATLLEVLM

Homology

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BLAST of MC05g0549 vs. ExPASy Swiss-Prot
Match: F6HDM2 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Vitis vinifera OX=29760 GN=VIT_05s0020g02800 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 3.1e-143
Identity = 261/343 (76.09%), Postives = 300/343 (87.46%), Query Frame = 0


Query: 15  LLRRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAG 74
           + RRQ+FLIR LG  G      R+   + M   ++EADAENI+RAITP+LD  R+KGQAG
Sbjct: 7   VFRRQEFLIRCLGVGGQSQQFYRKSIPRTM---ALEADAENILRAITPTLDLARHKGQAG 66

Query: 75  KIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYS 134
           KIAVIGGCREYTGAPYF+AISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+LEESYS
Sbjct: 67  KIAVIGGCREYTGAPYFSAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPLLEESYS 126

Query: 135 VRDEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVID 194
           VR+E+KK I+EKVL EV KW+ERFDCLV+GPGLGRDPFLL CVSEI+KHARQ+N+P+VID
Sbjct: 127 VREEDKKAISEKVLTEVVKWMERFDCLVVGPGLGRDPFLLGCVSEIMKHARQSNVPIVID 186

Query: 195 GDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGL 254
           GDGLFL+T++L LVS YPLAVLTPNVNEYKRLV+ VL  E+ +QDA++QLLSL K IGG+
Sbjct: 187 GDGLFLVTNSLDLVSGYPLAVLTPNVNEYKRLVQKVLNCEVGDQDAAEQLLSLAKGIGGV 246

Query: 255 TILRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLAT 314
           TILRKG++DLISDGETV SV IYGSPRRCGGQGDILSGSVAVF+SWA RQ +  +G+L  
Sbjct: 247 TILRKGKSDLISDGETVNSVGIYGSPRRCGGQGDILSGSVAVFLSWA-RQRIIAEGDLNI 306

Query: 315 SPKNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 358
           SPK+PT+LG IAGSAL+R+AASLAFENKKRSTLT DIIE L R
Sbjct: 307 SPKSPTVLGSIAGSALMRKAASLAFENKKRSTLTGDIIECLGR 345

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BLAST of MC05g0549 vs. ExPASy Swiss-Prot
Match: Q94AF2 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Arabidopsis thaliana OX=3702 GN=At5g19150 PE=1 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 6.1e-131
Identity = 246/350 (70.29%), Postives = 289/350 (82.57%), Query Frame = 0


Query: 9   PSKGEELLRRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNR 68
           PS    +LRRQ+FL+R+L G            I+AMS TS EADAE+++R +TPSLD  R
Sbjct: 19  PSSSSSVLRRQEFLVRTLCG---------SPIIRAMSSTS-EADAESVLRTVTPSLDLKR 78

Query: 69  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 128
           +KGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV
Sbjct: 79  HKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 138

Query: 129 LEESYSV---RDEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHAR 188
           LEESYS+    +E+K+ + +KVL EV KW+ERFDCLVIGPGLGRDPFLL+CVS I+  A+
Sbjct: 139 LEESYSISQLSEEDKREVQDKVLGEVGKWMERFDCLVIGPGLGRDPFLLECVSIIMLLAK 198

Query: 189 QTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLL 248
           ++N+P VIDGDGLFL+T+++ LV +YPLAVLTPNVNEYKRLV+ VL  E+DEQ+A  QL 
Sbjct: 199 KSNVPFVIDGDGLFLVTNSIDLVHSYPLAVLTPNVNEYKRLVQKVLNCEVDEQNAEDQLR 258

Query: 249 SLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQG 308
           SL K+IGG+TILRKG++DLIS+GETV+SVSIYGSPRRCGGQGDILSG VAVF+SWA  Q 
Sbjct: 259 SLAKQIGGVTILRKGKSDLISNGETVKSVSIYGSPRRCGGQGDILSGGVAVFLSWA--QQ 318

Query: 309 LATDGNLATSPKNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFL 356
           L +D    +  +NP ILGCIA S LLR+AASLAF   KRSTLT+DIIE L
Sbjct: 319 LKSDPE--SPSENPAILGCIAASGLLRKAASLAFTKHKRSTLTSDIIECL 354

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BLAST of MC05g0549 vs. ExPASy Swiss-Prot
Match: C5Y210 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Sorghum bicolor OX=4558 GN=Sb05g010020 PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 1.5e-116
Identity = 211/313 (67.41%), Postives = 253/313 (80.83%), Query Frame = 0


Query: 43  AMSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGAD 102
           A +GT  EADAE ++R ITP LD  R+KGQAGKIAVIGGCREYTGAPYFAAISALK+GAD
Sbjct: 74  ASAGTVYEADAEAVVRRITPPLDRARHKGQAGKIAVIGGCREYTGAPYFAAISALKVGAD 133

Query: 103 LSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLV 162
           LSHVFCTKDAA VIKSYSPELIVHP+LEESYSVRD+E+  ++ K+L EV KW+ERFDC+V
Sbjct: 134 LSHVFCTKDAATVIKSYSPELIVHPILEESYSVRDDERASVSSKILTEVGKWMERFDCIV 193

Query: 163 IGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNE 222
           +GPGLGRD FLLDCVS I++HARQ NIP V+DGDGLFL+T+NL LV   PLA+LTPNV E
Sbjct: 194 VGPGLGRDSFLLDCVSNIMRHARQANIPTVVDGDGLFLVTNNLSLVEGNPLAILTPNVYE 253

Query: 223 YKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRR 282
           YKRLV+ VL  ++DE+ AS+QL++L ++IG +TI++KG+AD+ISDG+TV  VS +GSPRR
Sbjct: 254 YKRLVQKVLNCDVDEETASEQLITLCQKIGDVTIMQKGKADVISDGKTVTQVSTFGSPRR 313

Query: 283 CGGQGDILSGSVAVFVSWARRQGLATDGNLATSPKNPTILGCIAGSALLRRAASLAFENK 342
           CGGQGDILSGSVAVF SWAR        +   + + PT           R+AAS AFE  
Sbjct: 314 CGGQGDILSGSVAVFASWAR--------HFVLTNEQPT---------EKRKAASHAFEKN 369

Query: 343 KRSTLTTDIIEFL 356
           KRST+T+DIIEFL
Sbjct: 374 KRSTVTSDIIEFL 369

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BLAST of MC05g0549 vs. ExPASy Swiss-Prot
Match: A9RY03 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_121160 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.6e-99
Identity = 197/337 (58.46%), Postives = 241/337 (71.51%), Query Frame = 0


Query: 44  MSGTSVEADAENIIRA---ITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIG 103
           M G S E   + I+ A   + P L P R+KGQAGKIAVIGGCREYTGAPYFAAISALK+G
Sbjct: 1   MGGNSTE--LQKILPALEKVVPVLAPGRHKGQAGKIAVIGGCREYTGAPYFAAISALKMG 60

Query: 104 ADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSV---RDEEKKFIAEKVLAEVDKWLER 163
           ADL+HVFCT  AA VIKSYSPELIVHPVL ESY V    +EE   + +KVLAEV KWL+R
Sbjct: 61  ADLAHVFCTSGAATVIKSYSPELIVHPVLHESYDVGEIGEEEISGLKDKVLAEVGKWLQR 120

Query: 164 FDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLT 223
           FDC+VIGPGLGRDP LLDCV+ II+ A+  NIP+V+DGDGLFL+T+   L+  YPLA+LT
Sbjct: 121 FDCIVIGPGLGRDPILLDCVAAIIEEAKFKNIPLVLDGDGLFLVTNQPELIIGYPLAILT 180

Query: 224 PNVNEYKRLVKNVL---------LAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDG 283
           PNV E+KRLV  ++           E+  +D   QL  L KR+ G+TIL+KG+ D ISDG
Sbjct: 181 PNVMEHKRLVAKIVGERDQNVPQNPEVSNEDLPGQLQDLAKRMEGVTILQKGKTDYISDG 240

Query: 284 ETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSPK-------NPTI 343
           +TV S   YGSPRRCGGQGD+LSGS AVFVSWA+     ++ N A   +       NPT+
Sbjct: 241 KTVLSSDYYGSPRRCGGQGDVLSGSTAVFVSWAKE--YFSNENAAGKKEVEERVSNNPTM 300

Query: 344 LGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWRR 359
           +G +AGS L R++A+ AF   KR+T TT+IIE+L  R
Sbjct: 301 VGALAGSLLCRKSAANAFAQHKRATTTTNIIEYLGHR 333

[Close]

BLAST of MC05g0549 vs. ExPASy Swiss-Prot
Match: E1BNQ4 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Bos taurus OX=9913 GN=NAXD PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.3e-72
Identity = 144/297 (48.48%), Postives = 193/297 (64.98%), Query Frame = 0


Query: 56  IIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPV 115
           ++R++ P+L   ++KGQ G+I V+GGCREYTGAPYFAAISALK+GADLSHVFCT++AAPV
Sbjct: 38  LVRSVVPALTTKKHKGQDGRIGVVGGCREYTGAPYFAAISALKVGADLSHVFCTQEAAPV 97

Query: 116 IKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLD 175
           IK+YSPELIVHPVL+   +VRD             V++WL R   LV+GPGLGRD  LL+
Sbjct: 98  IKAYSPELIVHPVLDSPEAVRD-------------VEQWLPRLHALVVGPGLGRDDALLE 157

Query: 176 CVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEI 235
            V  I++ ++   IP+VID DGL+LI     L+  Y  AVLTPN  E+ RL + VL   +
Sbjct: 158 NVKGILEASKARGIPVVIDADGLWLIAQQPALIQGYRKAVLTPNHVEFGRLSEAVLGVPL 217

Query: 236 DEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVA 295
           D  D    +L L + +G +T+++KG  D+ISDGE V   S  GS RRCGGQGD+LSGS+ 
Sbjct: 218 DGGDRHGAVLRLSQALGNVTVVQKGEQDVISDGEQVLECSQEGSGRRCGGQGDLLSGSLG 277

Query: 296 VFVSWARRQGLATDGNLATSPKNPTILGCIAGSALLRRAASLAFENKKRSTLTTDII 353
           V   WA R G    G       +P ++      AL R+ +  AF+   R+T T+D++
Sbjct: 278 VLAHWALRAGPQKTGG-----PSPLLVAAFGACALTRQCSQQAFQKYGRATTTSDMV 316

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BLAST of MC05g0549 vs. NCBI nr
Match: XP_023528365.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 649 bits (1675), Expect = 2.24e-233
Identity = 324/341 (95.01%), Postives = 334/341 (97.95%), Query Frame = 0


Query: 17  RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKI 76
           RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTS+EADAENI+RAITPSLDP RYKGQAGKI
Sbjct: 9   RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKRYKGQAGKI 68

Query: 77  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 136
           AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR
Sbjct: 69  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 128

Query: 137 DEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 196
           DEEKKF AE+VLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTNIPM+IDGD
Sbjct: 129 DEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTNIPMIIDGD 188

Query: 197 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTI 256
           GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAE+DEQDA  QLLSL KRIGGLTI
Sbjct: 189 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLTKRIGGLTI 248

Query: 257 LRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSP 316
           LRKGRADLI DGETV+SVSIYGSPRRCGGQGDILSGSVAVFVSWAR+QGL TDGNLATSP
Sbjct: 249 LRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVAVFVSWARQQGLVTDGNLATSP 308

Query: 317 KNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 357
           KNPT+LGCIAGSALLR+AASLAFENKKRSTLTTDIIEFLWR
Sbjct: 309 KNPTVLGCIAGSALLRKAASLAFENKKRSTLTTDIIEFLWR 349

[Close]

BLAST of MC05g0549 vs. NCBI nr
Match: XP_022955699.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita moschata])

HSP 1 Score: 647 bits (1670), Expect = 1.30e-232
Identity = 323/341 (94.72%), Postives = 333/341 (97.65%), Query Frame = 0


Query: 17  RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKI 76
           RRQQFLIRSLGGFGDC HQNRRQQIKAMSGTS+EADAENI+RAITPSLDP RYKGQAGKI
Sbjct: 9   RRQQFLIRSLGGFGDCAHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKRYKGQAGKI 68

Query: 77  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 136
           AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR
Sbjct: 69  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 128

Query: 137 DEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 196
           DEEKKF AE+VLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTNIPM+IDGD
Sbjct: 129 DEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTNIPMIIDGD 188

Query: 197 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTI 256
           GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAE+DEQDA  QLLSL KRIGGLTI
Sbjct: 189 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLTI 248

Query: 257 LRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSP 316
           LRKGRADLI DGETV+SVSIYGSPRRCGGQGDILSGSVAVFVSWAR+QGL TDGNLATSP
Sbjct: 249 LRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVAVFVSWARQQGLVTDGNLATSP 308

Query: 317 KNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 357
           KNPT+LGCIAGSALLR+AASLAFENKKRSTLTTDIIEFLWR
Sbjct: 309 KNPTVLGCIAGSALLRKAASLAFENKKRSTLTTDIIEFLWR 349

[Close]

BLAST of MC05g0549 vs. NCBI nr
Match: XP_022979515.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita maxima])

HSP 1 Score: 645 bits (1665), Expect = 7.49e-232
Identity = 323/341 (94.72%), Postives = 333/341 (97.65%), Query Frame = 0


Query: 17  RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKI 76
           RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTS+EADAENI+RAITPSLDP R+KGQAGKI
Sbjct: 9   RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKRHKGQAGKI 68

Query: 77  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 136
           AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR
Sbjct: 69  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 128

Query: 137 DEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 196
           DEEKKF AE+VLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTNIPMVIDGD
Sbjct: 129 DEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTNIPMVIDGD 188

Query: 197 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTI 256
           GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAE+DEQDA  QLLSL KRIGGLTI
Sbjct: 189 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLTI 248

Query: 257 LRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSP 316
           LRKGRADLI DGETV+SVSIYGSPRRCGGQGDILSGSVAVFVSWAR+QGL  DGNLATSP
Sbjct: 249 LRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVAVFVSWARQQGLVIDGNLATSP 308

Query: 317 KNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 357
           KNPT+LGCIAGSALLR+AASLAFENKKRSTLTTDIIEFLWR
Sbjct: 309 KNPTVLGCIAGSALLRKAASLAFENKKRSTLTTDIIEFLWR 349

[Close]

BLAST of MC05g0549 vs. NCBI nr
Match: KAG6582278.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 645 bits (1663), Expect = 1.57e-231
Identity = 324/342 (94.74%), Postives = 334/342 (97.66%), Query Frame = 0


Query: 17  RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKI 76
           RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTS+EADAENI+RAITPSLDP RYKGQAGKI
Sbjct: 9   RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKRYKGQAGKI 68

Query: 77  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 136
           AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR
Sbjct: 69  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 128

Query: 137 DEE-KKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDG 196
           DEE KKF AE+VLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTNIPM+IDG
Sbjct: 129 DEEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTNIPMIIDG 188

Query: 197 DGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLT 256
           DGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAE+DEQDA  QLLSL KRIGGLT
Sbjct: 189 DGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLT 248

Query: 257 ILRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATS 316
           ILRKGRADLI DGETV+SVSIYGSPRRCGGQGDILSGSVAVFVSWAR+QGL TDGNLATS
Sbjct: 249 ILRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVAVFVSWARQQGLVTDGNLATS 308

Query: 317 PKNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 357
           PKNPT+LGCIAGSALLR+AASLAFENKKRSTLTTDIIEFLWR
Sbjct: 309 PKNPTVLGCIAGSALLRKAASLAFENKKRSTLTTDIIEFLWR 350

[Close]

BLAST of MC05g0549 vs. NCBI nr
Match: XP_038878236.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase isoform X1 [Benincasa hispida])

HSP 1 Score: 640 bits (1651), Expect = 1.02e-229
Identity = 321/341 (94.13%), Postives = 331/341 (97.07%), Query Frame = 0


Query: 17  RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKI 76
           RRQQFLIRSLGGFGDCT+QN+RQQIKAMSG  +EADA NI+RAITPSLDPNRYKGQAGKI
Sbjct: 9   RRQQFLIRSLGGFGDCTYQNKRQQIKAMSGGYIEADAANILRAITPSLDPNRYKGQAGKI 68

Query: 77  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 136
           AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR
Sbjct: 69  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 128

Query: 137 DEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 196
           DEEKKFIAE+VLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEII+HARQ NIPMVIDGD
Sbjct: 129 DEEKKFIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIRHARQNNIPMVIDGD 188

Query: 197 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTI 256
           GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAE+DEQDA  QLLSL KRIGGLTI
Sbjct: 189 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLTI 248

Query: 257 LRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSP 316
           LRKGRADLISDGETV+SVSIYGSPRRCGGQGDILSGSVAVFVSWA+RQG  TDGNLATS 
Sbjct: 249 LRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGSVAVFVSWAQRQGFVTDGNLATSL 308

Query: 317 KNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 357
           KNPT+LGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR
Sbjct: 309 KNPTVLGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 349

[Close]

BLAST of MC05g0549 vs. ExPASy TrEMBL
Match: A0A6J1GX13 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111457620 PE=3 SV=1)

HSP 1 Score: 647 bits (1670), Expect = 6.28e-233
Identity = 323/341 (94.72%), Postives = 333/341 (97.65%), Query Frame = 0


Query: 17  RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKI 76
           RRQQFLIRSLGGFGDC HQNRRQQIKAMSGTS+EADAENI+RAITPSLDP RYKGQAGKI
Sbjct: 9   RRQQFLIRSLGGFGDCAHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKRYKGQAGKI 68

Query: 77  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 136
           AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR
Sbjct: 69  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 128

Query: 137 DEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 196
           DEEKKF AE+VLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTNIPM+IDGD
Sbjct: 129 DEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTNIPMIIDGD 188

Query: 197 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTI 256
           GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAE+DEQDA  QLLSL KRIGGLTI
Sbjct: 189 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLTI 248

Query: 257 LRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSP 316
           LRKGRADLI DGETV+SVSIYGSPRRCGGQGDILSGSVAVFVSWAR+QGL TDGNLATSP
Sbjct: 249 LRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVAVFVSWARQQGLVTDGNLATSP 308

Query: 317 KNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 357
           KNPT+LGCIAGSALLR+AASLAFENKKRSTLTTDIIEFLWR
Sbjct: 309 KNPTVLGCIAGSALLRKAASLAFENKKRSTLTTDIIEFLWR 349

[Close]

BLAST of MC05g0549 vs. ExPASy TrEMBL
Match: A0A6J1ITH2 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111479195 PE=3 SV=1)

HSP 1 Score: 645 bits (1665), Expect = 3.63e-232
Identity = 323/341 (94.72%), Postives = 333/341 (97.65%), Query Frame = 0


Query: 17  RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKI 76
           RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTS+EADAENI+RAITPSLDP R+KGQAGKI
Sbjct: 9   RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKRHKGQAGKI 68

Query: 77  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 136
           AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR
Sbjct: 69  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 128

Query: 137 DEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 196
           DEEKKF AE+VLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTNIPMVIDGD
Sbjct: 129 DEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTNIPMVIDGD 188

Query: 197 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTI 256
           GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAE+DEQDA  QLLSL KRIGGLTI
Sbjct: 189 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLTI 248

Query: 257 LRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSP 316
           LRKGRADLI DGETV+SVSIYGSPRRCGGQGDILSGSVAVFVSWAR+QGL  DGNLATSP
Sbjct: 249 LRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVAVFVSWARQQGLVIDGNLATSP 308

Query: 317 KNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 357
           KNPT+LGCIAGSALLR+AASLAFENKKRSTLTTDIIEFLWR
Sbjct: 309 KNPTVLGCIAGSALLRKAASLAFENKKRSTLTTDIIEFLWR 349

[Close]

BLAST of MC05g0549 vs. ExPASy TrEMBL
Match: A0A5D3CYW0 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00150 PE=3 SV=1)

HSP 1 Score: 637 bits (1644), Expect = 5.73e-229
Identity = 316/341 (92.67%), Postives = 332/341 (97.36%), Query Frame = 0


Query: 17  RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKI 76
           RRQQFL+R LGGFGDCT+QNRRQQIKAMSGTS+EADAE+I+RAITP LDPNRYKGQAGKI
Sbjct: 9   RRQQFLLRCLGGFGDCTYQNRRQQIKAMSGTSIEADAEHILRAITPCLDPNRYKGQAGKI 68

Query: 77  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 136
           AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR
Sbjct: 69  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 128

Query: 137 DEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 196
           DEEKKFI+E+VLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD
Sbjct: 129 DEEKKFISERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 188

Query: 197 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTI 256
           GLFLIT NLHLVSNYPLAVLTPNVNEYKRLV+NVLLAE+DEQDA  QLLSL KRIGG+TI
Sbjct: 189 GLFLITGNLHLVSNYPLAVLTPNVNEYKRLVENVLLAEVDEQDAPNQLLSLAKRIGGITI 248

Query: 257 LRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSP 316
           LRKGRADLISDGETV+SVSIYGSPRRCGGQGDILSGSVAVF+SWA+RQGL TD N+ +SP
Sbjct: 249 LRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGSVAVFISWAQRQGLVTDDNMTSSP 308

Query: 317 KNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 357
           KNPT+LGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR
Sbjct: 309 KNPTVLGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 349

[Close]

BLAST of MC05g0549 vs. ExPASy TrEMBL
Match: A0A1S3AX65 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo OX=3656 GN=LOC103483736 PE=3 SV=1)

HSP 1 Score: 637 bits (1643), Expect = 8.14e-229
Identity = 316/341 (92.67%), Postives = 332/341 (97.36%), Query Frame = 0


Query: 17  RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKI 76
           RRQQFL+R LGGFGDCT+QNRRQQIKAMSGTS+EADAE+I+RAITP LDPNRYKGQAGKI
Sbjct: 9   RRQQFLLRCLGGFGDCTYQNRRQQIKAMSGTSIEADAEHILRAITPCLDPNRYKGQAGKI 68

Query: 77  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 136
           AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR
Sbjct: 69  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 128

Query: 137 DEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 196
           DEE+KFI+E+VLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD
Sbjct: 129 DEERKFISERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 188

Query: 197 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTI 256
           GLFLIT NLHLVSNYPLAVLTPNVNEYKRLV+NVLLAE+DEQDA  QLLSL KRIGGLTI
Sbjct: 189 GLFLITGNLHLVSNYPLAVLTPNVNEYKRLVENVLLAEVDEQDAPNQLLSLAKRIGGLTI 248

Query: 257 LRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSP 316
           LRKGRADLISDGETV+SVSIYGSPRRCGGQGDILSGSVAVF+SWA+RQGL TD N+ +SP
Sbjct: 249 LRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGSVAVFISWAQRQGLVTDDNMTSSP 308

Query: 317 KNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 357
           KNPT+LGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR
Sbjct: 309 KNPTVLGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 349

[Close]

BLAST of MC05g0549 vs. ExPASy TrEMBL
Match: A0A0A0L7Q1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis sativus OX=3659 GN=Csa_3G149940 PE=3 SV=1)

HSP 1 Score: 634 bits (1634), Expect = 1.91e-227
Identity = 316/341 (92.67%), Postives = 331/341 (97.07%), Query Frame = 0


Query: 17  RRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNRYKGQAGKI 76
           RRQQFL+RSLGGFGDCT+QNRR QIKAMSGTS+EADA+ I+RAITP LDPNRYKGQAGKI
Sbjct: 9   RRQQFLLRSLGGFGDCTYQNRRLQIKAMSGTSIEADADLILRAITPCLDPNRYKGQAGKI 68

Query: 77  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 136
           AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR
Sbjct: 69  AVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVR 128

Query: 137 DEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 196
           DEEKKFIAE+VLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD
Sbjct: 129 DEEKKFIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGD 188

Query: 197 GLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTI 256
           GLFLIT NLHLVSNYPLAVLTPNVNEYKRLV+NVLLAE+DEQDA  QLL+L KRIGGLTI
Sbjct: 189 GLFLITGNLHLVSNYPLAVLTPNVNEYKRLVRNVLLAEVDEQDAPNQLLTLAKRIGGLTI 248

Query: 257 LRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSP 316
           LRKGRADLISDGETV+SVSIYGSPRRCGGQGDILSGSVAVF+SWA+RQG  TD NL++SP
Sbjct: 249 LRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGSVAVFISWAQRQGSITDDNLSSSP 308

Query: 317 KNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 357
           KNPT+LGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR
Sbjct: 309 KNPTVLGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWR 349

[Close]

BLAST of MC05g0549 vs. TAIR 10
Match: AT5G19150.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 468.8 bits (1205), Expect = 4.3e-132
Identity = 246/350 (70.29%), Postives = 289/350 (82.57%), Query Frame = 0


Query: 9   PSKGEELLRRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNR 68
           PS    +LRRQ+FL+R+L G            I+AMS TS EADAE+++R +TPSLD  R
Sbjct: 19  PSSSSSVLRRQEFLVRTLCG---------SPIIRAMSSTS-EADAESVLRTVTPSLDLKR 78

Query: 69  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 128
           +KGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV
Sbjct: 79  HKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 138

Query: 129 LEESYSV---RDEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHAR 188
           LEESYS+    +E+K+ + +KVL EV KW+ERFDCLVIGPGLGRDPFLL+CVS I+  A+
Sbjct: 139 LEESYSISQLSEEDKREVQDKVLGEVGKWMERFDCLVIGPGLGRDPFLLECVSIIMLLAK 198

Query: 189 QTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLL 248
           ++N+P VIDGDGLFL+T+++ LV +YPLAVLTPNVNEYKRLV+ VL  E+DEQ+A  QL 
Sbjct: 199 KSNVPFVIDGDGLFLVTNSIDLVHSYPLAVLTPNVNEYKRLVQKVLNCEVDEQNAEDQLR 258

Query: 249 SLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQG 308
           SL K+IGG+TILRKG++DLIS+GETV+SVSIYGSPRRCGGQGDILSG VAVF+SWA  Q 
Sbjct: 259 SLAKQIGGVTILRKGKSDLISNGETVKSVSIYGSPRRCGGQGDILSGGVAVFLSWA--QQ 318

Query: 309 LATDGNLATSPKNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFL 356
           L +D    +  +NP ILGCIA S LLR+AASLAF   KRSTLT+DIIE L
Sbjct: 319 LKSDPE--SPSENPAILGCIAASGLLRKAASLAFTKHKRSTLTSDIIECL 354

[Close]

BLAST of MC05g0549 vs. TAIR 10
Match: AT5G19150.2 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 468.8 bits (1205), Expect = 4.3e-132
Identity = 246/350 (70.29%), Postives = 289/350 (82.57%), Query Frame = 0


Query: 9   PSKGEELLRRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSVEADAENIIRAITPSLDPNR 68
           PS    +LRRQ+FL+R+L G            I+AMS TS EADAE+++R +TPSLD  R
Sbjct: 19  PSSSSSVLRRQEFLVRTLCG---------SPIIRAMSSTS-EADAESVLRTVTPSLDLKR 78

Query: 69  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 128
           +KGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV
Sbjct: 79  HKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 138

Query: 129 LEESYSV---RDEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHAR 188
           LEESYS+    +E+K+ + +KVL EV KW+ERFDCLVIGPGLGRDPFLL+CVS I+  A+
Sbjct: 139 LEESYSISQLSEEDKREVQDKVLGEVGKWMERFDCLVIGPGLGRDPFLLECVSIIMLLAK 198

Query: 189 QTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLL 248
           ++N+P VIDGDGLFL+T+++ LV +YPLAVLTPNVNEYKRLV+ VL  E+DEQ+A  QL 
Sbjct: 199 KSNVPFVIDGDGLFLVTNSIDLVHSYPLAVLTPNVNEYKRLVQKVLNCEVDEQNAEDQLR 258

Query: 249 SLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQG 308
           SL K+IGG+TILRKG++DLIS+GETV+SVSIYGSPRRCGGQGDILSG VAVF+SWA  Q 
Sbjct: 259 SLAKQIGGVTILRKGKSDLISNGETVKSVSIYGSPRRCGGQGDILSGGVAVFLSWA--QQ 318

Query: 309 LATDGNLATSPKNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFL 356
           L +D    +  +NP ILGCIA S LLR+AASLAF   KRSTLT+DIIE L
Sbjct: 319 LKSDPE--SPSENPAILGCIAASGLLRKAASLAFTKHKRSTLTSDIIECL 354

The following BLAST results are available for this feature:

BLAST of MC05g0549 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F6HDM2	3.1e-143	76.09	ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Vitis vinifera OX=29760 GN=VIT_...	[more]
Q94AF2	6.1e-131	70.29	ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Arabidopsis thaliana OX=3702 GN...	[more]
C5Y210	1.5e-116	67.41	ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Sorghum bicolor OX=4558 GN=Sb05...	[more]
A9RY03	1.6e-99	58.46	ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Physcomitrium patens OX=3218 GN...	[more]
E1BNQ4	1.3e-72	48.48	ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Bos taurus OX=9913 GN=NAXD PE=3...	[more]

BLAST of MC05g0549 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023528365.1	2.24e-233	95.01	ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita pepo subsp. pepo]	[more]
XP_022955699.1	1.30e-232	94.72	ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita moschata]	[more]
XP_022979515.1	7.49e-232	94.72	ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita maxima]	[more]
KAG6582278.1	1.57e-231	94.74	ATP-dependent (S)-NAD(P)H-hydrate dehydratase, partial [Cucurbita argyrosperma s...	[more]
XP_038878236.1	1.02e-229	94.13	ATP-dependent (S)-NAD(P)H-hydrate dehydratase isoform X1 [Benincasa hispida]	[more]

BLAST of MC05g0549 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GX13	6.28e-233	94.72	ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1ITH2	3.63e-232	94.72	ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A5D3CYW0	5.73e-229	92.67	ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3AX65	8.14e-229	92.67	ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A0A0L7Q1	1.91e-227	92.67	ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis sativus OX=3659 GN=Csa_...	[more]

BLAST of MC05g0549 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19150.1	4.3e-132	70.29	pfkB-like carbohydrate kinase family protein	[more]
AT5G19150.2	4.3e-132	70.29	pfkB-like carbohydrate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029056	Ribokinase-like	GENE3D	3.40.1190.20		coord: 52..359 e-value: 1.5E-79 score: 269.2
IPR029056	Ribokinase-like	SUPERFAMILY	53613	Ribokinase-like	coord: 55..357
IPR000631	ATP-dependent (S)-NAD(P)H-hydrate dehydratase	TIGRFAM	TIGR00196	TIGR00196	coord: 59..300 e-value: 1.2E-48 score: 163.8
IPR000631	ATP-dependent (S)-NAD(P)H-hydrate dehydratase	PFAM	PF01256	Carb_kinase	coord: 77..354 e-value: 5.5E-50 score: 170.1
IPR000631	ATP-dependent (S)-NAD(P)H-hydrate dehydratase	HAMAP	MF_01965	NADHX_dehydratase	coord: 53..364 score: 28.552046
IPR000631	ATP-dependent (S)-NAD(P)H-hydrate dehydratase	PROSITE	PS51383	YJEF_C_3	coord: 53..363 score: 74.381546
IPR000631	ATP-dependent (S)-NAD(P)H-hydrate dehydratase	CDD	cd01171	YXKO-related	coord: 66..353 e-value: 1.66425E-83 score: 253.304
None	No IPR available	PANTHER	PTHR12592	ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER	coord: 13..356
None	No IPR available	PANTHER	PTHR12592:SF1	ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE	coord: 13..356

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0549.1	MC05g0549.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0110051	metabolite repair
biological_process	GO:0046496	nicotinamide nucleotide metabolic process
molecular_function	GO:0052855	ADP-dependent NAD(P)H-hydrate dehydratase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0047453	ATP-dependent NAD(P)H-hydrate dehydratase activity