MC05g0527 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0527
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description5'-nucleotidase SurE
LocationMC05: 3978485 .. 3983847 (+)
RNA-Seq ExpressionMC05g0527
SyntenyMC05g0527
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCACTTTTAAAATATTCATTTTTAGTTTCCTTGCAAAAAGGAAAACAGATGTAAATTTCCAAAAGGCGGAAACGACGAGAAGTAGAACAAAATTACAATAATTGCGTCAGCGGAGCGCTTTGATTGGCTATGGAGTCTCAGATATTCTTCCGAATAATGGTTTTTTAAATCAGTCCTCTGTGCCCGAGGCCATACTCTAGTTTCTCTTCTCTTCTTCTACGTTGCTCCGGCGTCCTCCTGCGTCTCTGTGCGCAAAAATGGAAGCCACCGCCGTCAGCTCAGACAGTAGACCGACGATCATGGTCACCAACGACGATGGAATCGATGCGCCCGGTCTTCGCGCTCTGGTTCGCGTCCTCGTCTCAACTCAGCTCTACAATGTTCAAGTCTGCGCTCCTGATTCGTACGCCTCTTATCCTCTTTCTCCTCCTGCTCTTCCTGTTCCAATTTGCGTGCGCTGCCGGCTGAAACTTATGATTATGATCTGTGTTTCAGATACGTGCGTTTTAGTTTGTGAAATTTTGTTCATACGTCTGTTCTGTTTGCTGGTTCTAATTTGACTGATCGCTAGTGCACTTAGAGTTCTTTTATCATTTTGCTAGTGATTATGCTAACTTCTTTCTTTTAGAATGTCGATGGCCATCGGATCGCGTGATTTGAGATATAAGCAGCTGTTTTCTTCTGTACAGTTTTCTTATTTATTAGTCAATATTATAAGATTGGGTTGTAGGTCCCGAATTATTAGCTTCATTTTGTTTTATTTGATAATTGTTATTATTATTATTATTATGACGTTGTGGGGGGTTGTTTTTGCAGGGAAAAATCAGCTGTTAGCCAATCTATTACTTGGCGTCATCCTGTTTCTGTTAAGAAAGTCGCTATTGAAGGAACAACTTCTTATGCTGTTTCTGGTCTGTCTTCTATTCCTCTCTTTTGAAATCTTTAATTTCGTTTGAACTAGTGCACACTGCAGATTTTGTTGTGCCTTTGAGTTTAGTTCTAGGAGTTGGTAGGAAGAATGTATTTGGATATCGATGATAAATGAATTCACTTGCTGGTGAAAGATTTAACTTTCTCTCCTCAACTTGAATGGCAGCAGGGTTTCTGACATCCTTGTTTTCAAATGCTTTATCTTTTACAAGAAACTTAAATAGACTGTTCACTTCTCAGCTATTCTACCTCACCTGGAAATGTTTAGTTCGTTGGAGGTTCTGGGAAAAATAATTCTTTAATAGGCCTATAACCCCAAGCCCCTGATGGTTAGTTCGTGGAAGTGTCTACTTTCTTATGTTCTACGTATAAACACAATGTTGGTGCTCGTGACAATGCTATGAAGCTATAGTAGTGTGGCAGAGTAAGGGAGAGTTAAAACCTTTACAGTAATTATGCTCAAATGGGGCCTTACCAATATATTAGGACCATTAATGTACATTAAGATATTTGCTATAGTCCAATGTCCAATTACTTGTCTCTTCCTCCAATATTTGTTTTATTTTCACTTTTACTGAATGTATTTAACTTGAAGCTTTGGGGTTGTAATGAAATGGACTGAAATGATCTGCCATGCACTCTCCCTCCTTGAGACATAATGTTGAAGTATTTCTCGTTTTCATCAAAACCAATTATCATTGACTTGAACTTCAACCTAGCTGATGCCCGATATTGCTTCTAGTGACTAACGAGGTTTTAAGCTTTTGTGCTTCTGTAGGAACTCCAGCCGATTGTACTTCTTTGGGAATATCCAAAGCTCTCTTTCCTTCAATTCCTGATCTGGTAGTTCTCTTTTCGTTTAGTGCATGGTTTGGCTGTAGCCTGTAGTATATTTTTTTTTCATATAGAGCTATTTCTATTTGTGCTTTGTTCACTTTAATTTATTTGACGTGTGGATAAGCTGACAAGAAGTTAGGATTTATAATGACACAAGAAATGTCTTTTCAACCCAGATGTGTATCTGTAATTGTAAATGTAGATTATTTTGTCAAACAACTACTTTATGTTGGATGGTCACTCTTCTCACAATGTTATCACTAATGAAGTTGGTGCATACAACTTGTAATCTGTTTAGCTCTTCTTGCCTATTTATGAATTGTACTTTTAATTGCAGGTAGTCAGTGGCATAAACATGGGTAGCAACTGCGGTTATCACGTGTTAGTGACTAATCTTGACAGATTTGCCCTTATATTGAACTATTTTGACAGCTTAACTATTAATGGGCACTGAAGTATCAAATATGTGCTCAGCATGCGTATTTACATGCCCATAGTATTGGCAAGAAAAGTTTGCACTTAAAGTATCAACTTGTGTGGAACCCATTAAAGCTTTCATACTTATTTGTCTAGCAAGTAAAATGAGAGCTATCTGTGCGTGGTTGGAAATGATTTTTTAACTTTGTTGATTCAGTCTTTCATTTAAACTGCAGTGTATATTCTGGAACGGTAGCTGGTGCTCGAGAAGCCTTTTTTAATGGCATTCCTTCTATTTCTTTATCTTATGAGTGGTATGTAGAAAGGTTTCCTTTATTGCTAGACTTAATCATGTAATTGTTACGTAATAACTAATATCTATTGGGATAGATTCCCTTTTTTCTCTTTTCTTTTTTTTTTCCCAAAAGAAAGGTTCCAAAGTGTTGAGCAGAAGATTGTCTGTGATCTTGGTTAAAATTTTGTTGATCATCTTAATTATCACTCTAAAAGTATATCTCATTATTATAGGTTGTATTATTAGTCTTTACTCATTTGAATTTTCTTGTTATGGAGCTTCAGGGTTGGGGGTAGGAGCAATATAAATGACTACACACTCGCTGCTGAGGCTTGCTTGCCCATAATAAGTGCAATGTTAGCTGATGCCAAGGCTCAAACTTTTCCACGAAGTTGTTTCTTGAATATTGATTTTCCAACAGATATTGCTAACCATAGGGTAATAAATATTATAACACCTAGTGAACATCTTGTGCCATCTTGAATTGAGATGTGCTCTAGAAAGTATTATGTTGACTGCTTATTCTACTTCCATGTTATGAAAGAGGTGGGGTAACAGTAATACTGGTGGATGTTTTTTTCCTTGTACATTATTTAGGGGTATAAGGTAACCAAACAAGGTCGATGCATGTACAAAATGGGTTGGAGAAGAGTTGCTTCTGATTCTCAAGGAGGTAAAATGTTGTCAACAATGACGATGGAGCCAGCAGCATCAGTAGCGGAATGTTATATGACAAATGAATCATCTGAACTGTTTACGAGGCAGGTTGGTACTCTTCTCCTCCCTCCTCCAAATTCTATAATTCAAGAAACCCATTAATTCCTTCTATTCTATCTATCGGGATCAATTATCAATTCTAGTTTAGAAATGCTATTGGAAGTTAGCCTCTGGTTGCTTATCTATTATTGAAAATGAAGGACCATAAAATAAAACATGGTTGTCATGTTCACTTTATTTCTCCCTAACGACACCCTTGTCCTCGAGTGTGAAATTCGAGGATGATTCTTTGATATCTTCACCTCAAAATGCTGCACTGAATTCCTTGAATGTATGCAAGAGTTTCTCTTAACCCAACAACATGAAAGGGCTCCGTTATGCATTCAGAAAACATTGCTTCACTATTAAGTTGAGGTCTACAGTTTTGTCTTCTGTTGTAAGTGGTCCAAATATTGCAAACCTGTATCTAGACGAAAAATGTAAAGGAAAATGAACTTGTAGGAGGGAGGTTTAACCTCCAAATTAGCCAATTAGAGAAACTCTAACTTCCCAATAGTTAATACAGAGAGTGCAACAATAAAAATTTCCGTACTTATCTATTCATTTCATCATTATGAATGGAAATCTCTAGAACACTGGGACTGTTCTGTTCTAGATTGCTCATTCTCTGCAACATTTAACCATCAGGTTGCACCCATTTGCATTCTCAGTTGGAATTATTTTATTTTGGATAATTTGATGACTCTTTTATTTAACCATTCCTAGCTTTGCGTCACTCAGAATGTTATATTTGTAAATGTTAACTTTAAGTTATTATCTTTGTAGGTAATAGGTGCACCTGTTGATGACGAAGATACAGATTTCAAATATCTACGAGAAGGCTACGTGTGTATCATTCTCTTTAGAAAATTAACATGTTTTTGTACTAGAAAATAGAAATTATTACAGAAGATATTATCATCATTAATTAGAAGCTCGTGATTCCATTTCAGATTACCGTCACTCCTCTTGCTGCTCTGTCTCGTGCAGATATAGAATGCCAAAACTTTTTGGAAGCTTGGTTGCCTGGTGTGGCTGCTCAGCCGTCTCCATCTGCTTTATAATTCCGTAAGGTTTCTGGTTAAAGTTCTATGAACTTCCTTCGTTTCATTTTGTTTAAATACCAATAAGAAGATAGACGATAAACACATAGGAGCCGTACAGCCTCAGCTCATGAGTCGTAATTATCTTTTCCCTTCCCCAATCAATAGAAATATTTAGTCCATGTCCATATTATTATTGCGTGAAGCTCATTATCCTTTCTGCTTCCTCAACGGTGCAGACGTTTTAGGCAGCTAGTCTGCAACCAATTTATAACTTCTGCAGTACCCTTATGTTCAACTATAATTTATGTTGAACTCCAGACATGGTATCTAAATTTGGTTAAAATAATGTTGGCAGTCTCAGTATTCTGTTTATCAGTCTTGAATGAACAGTAGTTTTTTCTGTGGGGACTTGACTTGACTTAAATGAAAACTCGATAACTTGCACGTGCTCTGCAGGAACCATAACAGATCTGATGATCTATTCTATTGAAGGATAGTACAACATGAGTGTTCATTCAAGGTTCTTTCCACTCTACTGCTGAACAAAACATTACCAAAACAAGTGTTACTCAAAATTTTCCGTTTTATTCTTTCCAGGTTTCAGCATTTTGTTAGAAGATCAAACCTCGAGCGGAGTAGGGTTTCTTATTTACTATCATTTGTTTCGTGCTGCCAGAGAGCGCAGGTAACTGTGTCATGAAGAAATATAGGTCCCGACTGATGAACTGTTACTGTTGACTGATTCTTTTAAAACATTCACTCAATTGTGACACAAACTTGAAGAAAGGGTCTAATATTCTTATGATTGTTGTTTAGTTCCAGTAAGGTGTATAGTTATTTGCTCTGGTTTCTTGTTATTATTACAAATCTTTGTGAGTTGTGACTTGAAAAGTAACAGAGAGAGAGTCAAATTAGAATTATTGATGAAAATATTAGAGAGAATTTGTTGCTCCATGGAAGATGGTATAAACTATATGAGATGTTAGAGTCATGAAGGTTTTAGGTACCCATTTATTGAGTTAGAGTGAGATAGTTCCTACACTCTAAGAATGTCTTAGGAAATAAATGTTTGGTTCAGGTT

mRNA sequence

GTCACTTTTAAAATATTCATTTTTAGTTTCCTTGCAAAAAGGAAAACAGATGTAAATTTCCAAAAGGCGGAAACGACGAGAAGTAGAACAAAATTACAATAATTGCGTCAGCGGAGCGCTTTGATTGGCTATGGAGTCTCAGATATTCTTCCGAATAATGGTTTTTTAAATCAGTCCTCTGTGCCCGAGGCCATACTCTAGTTTCTCTTCTCTTCTTCTACGTTGCTCCGGCGTCCTCCTGCGTCTCTGTGCGCAAAAATGGAAGCCACCGCCGTCAGCTCAGACAGTAGACCGACGATCATGGTCACCAACGACGATGGAATCGATGCGCCCGGTCTTCGCGCTCTGGTTCGCGTCCTCGTCTCAACTCAGCTCTACAATGTTCAAGTCTGCGCTCCTGATTCGGAAAAATCAGCTGTTAGCCAATCTATTACTTGGCGTCATCCTGTTTCTGTTAAGAAAGTCGCTATTGAAGGAACAACTTCTTATGCTGTTTCTGGAACTCCAGCCGATTGTACTTCTTTGGGAATATCCAAAGCTCTCTTTCCTTCAATTCCTGATCTGGTAGTCAGTGGCATAAACATGGGTAGCAACTGCGGTTATCACGTGTTAGTGACTAATCTTGACAGATTTGCCCTTATATTGAACTATTTTGACAGCTTAACTATTAATACTGAAGTATCAAATATTGTATATTCTGGAACGGTAGCTGGTGCTCGAGAAGCCTTTTTTAATGGCATTCCTTCTATTTCTTTATCTTATGAGTGGGTTGGGGGTAGGAGCAATATAAATGACTACACACTCGCTGCTGAGGCTTGCTTGCCCATAATAAGTGCAATGTTAGCTGATGCCAAGGCTCAAACTTTTCCACGAAGTTGTTTCTTGAATATTGATTTTCCAACAGATATTGCTAACCATAGGGGGTATAAGGTAACCAAACAAGGTCGATGCATGTACAAAATGGGTTGGAGAAGAGTTGCTTCTGATTCTCAAGGAGGTAAAATGTTGTCAACAATGACGATGGAGCCAGCAGCATCAGTAGCGGAATGTTATATGACAAATGAATCATCTGAACTGTTTACGAGGCAGGTTGGTGCACCTGTTGATGACGAAGATACAGATTTCAAATATCTACGAGAAGGCTACATTACCGTCACTCCTCTTGCTGCTCTGTCTCGTGCAGATATAGAATGCCAAAACTTTTTGGAAGCTTGGTTGCCTGGTGTGGCTGCTCAGCCGTCTCCATCTGCTTTATAATTCCGTAAGGTTTCTGGTTAAAGTTCTATGAACTTCCTTCGTTTCATTTTGTTTAAATACCAATAAGAAGATAGACGATAAACACATAGGAGCCGTACAGCCTCAGCTCATGAGTCGTAATTATCTTTTCCCTTCCCCAATCAATAGAAATATTTAGTCCATGTCCATATTATTATTGCGTGAAGCTCATTATCCTTTCTGCTTCCTCAACGGTGCAGACGTTTTAGGCAGCTAGTCTGCAACCAATTTATAACTTCTGCAGTACCCTTATGTTCAACTATAATTTATGTTGAACTCCAGACATGGTATCTAAATTTGGTTAAAATAATGTTGGCAGTCTCAGTATTCTGTTTATCAGTCTTGAATGAACAGTAGTTTTTTCTGTGGGGACTTGACTTGACTTAAATGAAAACTCGATAACTTGCACGTGCTCTGCAGGAACCATAACAGATCTGATGATCTATTCTATTGAAGGATAGTACAACATGAGTGTTCATTCAAGGTTTCAGCATTTTGTTAGAAGATCAAACCTCGAGCGGAGTAGGGTTTCTTATTTACTATCATTTGTTTCGTGCTGCCAGAGAGCGCAGGTAACTGTGTCATGAAGAAATATAGGTCCCGACTGATGAACTGTTACTGTTGACTGATTCTTTTAAAACATTCACTCAATTGTGACACAAACTTGAAGAAAGGGTCTAATATTCTTATGATTGTTGTTTAGTTCCAGTAAGGTGTATAGTTATTTGCTCTGGTTTCTTGTTATTATTACAAATCTTTGTGAGTTGTGACTTGAAAAGTAACAGAGAGAGAGTCAAATTAGAATTATTGATGAAAATATTAGAGAGAATTTGTTGCTCCATGGAAGATGGTATAAACTATATGAGATGTTAGAGTCATGAAGGTTTTAGGTACCCATTTATTGAGTTAGAGTGAGATAGTTCCTACACTCTAAGAATGTCTTAGGAAATAAATGTTTGGTTCAGGTT

Coding sequence (CDS)

ATGGAAGCCACCGCCGTCAGCTCAGACAGTAGACCGACGATCATGGTCACCAACGACGATGGAATCGATGCGCCCGGTCTTCGCGCTCTGGTTCGCGTCCTCGTCTCAACTCAGCTCTACAATGTTCAAGTCTGCGCTCCTGATTCGGAAAAATCAGCTGTTAGCCAATCTATTACTTGGCGTCATCCTGTTTCTGTTAAGAAAGTCGCTATTGAAGGAACAACTTCTTATGCTGTTTCTGGAACTCCAGCCGATTGTACTTCTTTGGGAATATCCAAAGCTCTCTTTCCTTCAATTCCTGATCTGGTAGTCAGTGGCATAAACATGGGTAGCAACTGCGGTTATCACGTGTTAGTGACTAATCTTGACAGATTTGCCCTTATATTGAACTATTTTGACAGCTTAACTATTAATACTGAAGTATCAAATATTGTATATTCTGGAACGGTAGCTGGTGCTCGAGAAGCCTTTTTTAATGGCATTCCTTCTATTTCTTTATCTTATGAGTGGGTTGGGGGTAGGAGCAATATAAATGACTACACACTCGCTGCTGAGGCTTGCTTGCCCATAATAAGTGCAATGTTAGCTGATGCCAAGGCTCAAACTTTTCCACGAAGTTGTTTCTTGAATATTGATTTTCCAACAGATATTGCTAACCATAGGGGGTATAAGGTAACCAAACAAGGTCGATGCATGTACAAAATGGGTTGGAGAAGAGTTGCTTCTGATTCTCAAGGAGGTAAAATGTTGTCAACAATGACGATGGAGCCAGCAGCATCAGTAGCGGAATGTTATATGACAAATGAATCATCTGAACTGTTTACGAGGCAGGTTGGTGCACCTGTTGATGACGAAGATACAGATTTCAAATATCTACGAGAAGGCTACATTACCGTCACTCCTCTTGCTGCTCTGTCTCGTGCAGATATAGAATGCCAAAACTTTTTGGAAGCTTGGTTGCCTGGTGTGGCTGCTCAGCCGTCTCCATCTGCTTTATAA

Protein sequence

MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITWRHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVTNLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDYTLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRVASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQVGAPVDDEDTDFKYLREGYITVTPLAALSRADIECQNFLEAWLPGVAAQPSPSAL
Homology
BLAST of MC05g0527 vs. ExPASy Swiss-Prot
Match: A6LS61 (5'-nucleotidase SurE OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) OX=290402 GN=surE PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.8e-27
Identity = 93/293 (31.74%), Postives = 146/293 (49.83%), Query Frame = 0

Query: 14  IMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITWRHPVSVKKVAIEG 73
           I++TNDDGI+APG+ AL + +  ++ + V + AP  +KSA S SI+   P+ +K+  IEG
Sbjct: 3   ILITNDDGINAPGIIALAKEI--SKEHKVTIVAPKDQKSASSHSISIHSPIKIKEEFIEG 62

Query: 74  --TTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVTNLDRFALILNY 133
               +Y+VSGTPADCT +G+S        +LV+SGIN G N G                 
Sbjct: 63  LDCKAYSVSGTPADCTQVGLS--FLKENIELVISGINKGPNVG----------------- 122

Query: 134 FDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDYTLAAEACLPII 193
                     ++I+YSGTV+ A E    GIPSI++S +   G+ +  DY+ A +  + ++
Sbjct: 123 ----------TDILYSGTVSAAIEGTIYGIPSIAVSMDVEYGKDD-EDYSKAVKWAIKVL 182

Query: 194 SAMLADAKAQTFPRSCFLNIDFPT-DIANHRGYKVTKQGRCMYKMGWRRVASDSQGGKML 253
                 AK +       LN++ P  +  + +G KV K GR  YK  +  + S        
Sbjct: 183 DI----AKEEYLKSDVVLNLNIPNFNERDIKGIKVCKIGRSTYKTEYILLES-------- 234

Query: 254 STMTMEPAASVAECYMTNESSELF-TRQVGAPVDDEDTDFKYLREGYITVTPL 303
                            NE  +L+ T+     + +E++D  YL +GY+T+TPL
Sbjct: 243 -----------------NEEGDLYTTKGTRNAIKEEESDLYYLYQGYVTLTPL 234

BLAST of MC05g0527 vs. ExPASy Swiss-Prot
Match: Q1J2E1 (5'-nucleotidase SurE OS=Deinococcus geothermalis (strain DSM 11300 / AG-3a) OX=319795 GN=surE PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.2e-26
Identity = 107/317 (33.75%), Postives = 147/317 (46.37%), Query Frame = 0

Query: 4   TAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITWRHP 63
           TA+ S  RP I+V NDDGI +PG++AL   L    + +V V APD E+SAV   IT R P
Sbjct: 2   TALPSPDRPRILVANDDGIFSPGIKAL--ALALADVGDVVVVAPDVEQSAVGHGITIRRP 61

Query: 64  VSVKKVAIEG---TTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 123
           +  K  A  G     +Y V GTPADC  LG+        PDLVVSGIN+GSN G      
Sbjct: 62  LRFKHTASAGFGTLPAYRVDGTPADCVVLGVH---LLGRPDLVVSGINLGSNLG------ 121

Query: 124 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 183
                       D LT         +SGTVA A E    G+PSI+ S +  GG     +Y
Sbjct: 122 ------------DDLT---------HSGTVAAAIEGLALGLPSIAFSQQGNGG----GEY 181

Query: 184 TLAAEACLPIISAMLADA-KAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRR 243
           +  A A     +A LA A  A+  P    LN++FP  +   RG ++TK G   ++     
Sbjct: 182 SFTAGAA---YAARLARAVLAKGLPPRVLLNVNFPAGLP--RGVRITKVGEHRWED---- 241

Query: 244 VASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQVGAPVDDEDTDFKYLREGYITV 303
                      S +T          ++  +S         A   D DTD+  ++ GY++V
Sbjct: 242 -----------SIITRHDPEGREYHWVAGQSR-------AADAHDPDTDYGAVQAGYVSV 251

Query: 304 TPLAALSRADIECQNFL 317
           TP+    R D+  ++ L
Sbjct: 302 TPV----RLDLTARDLL 251

BLAST of MC05g0527 vs. ExPASy Swiss-Prot
Match: A6LL96 (5'-nucleotidase SurE OS=Thermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429) OX=391009 GN=surE PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.9e-25
Identity = 93/292 (31.85%), Postives = 141/292 (48.29%), Query Frame = 0

Query: 14  IMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITWRHPVSVKKVAI-E 73
           I+VTNDDG+ A G+  L R L  ++ + V V AP++E+SAV  +IT R P+ ++K+ I E
Sbjct: 3   ILVTNDDGVTADGILCLARYL--SKKHEVTVVAPETEQSAVGHAITLRFPLWLRKIDINE 62

Query: 74  GTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVTNLDRFALILNYF 133
               YAVSGTPADC  +GI   L    PDL++SGIN G+N G                  
Sbjct: 63  EFEIYAVSGTPADCVKMGIDVVL-KEKPDLLISGINRGNNLG------------------ 122

Query: 134 DSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDYTLAAEACLPIIS 193
                    +++VYSGTV+GA E    G+PSI++        S+ +      E     I 
Sbjct: 123 ---------TDVVYSGTVSGALEGAIAGVPSIAI--------SSFSFENPLYETAAKFIL 182

Query: 194 AMLADAKAQTFPRSCFLNIDFPT-DIANHRGYKVTKQGRCMYKMGWRRVASDSQGGKMLS 253
             L +   ++ PR   LNI+ P+      +G+K+T+Q + MY+  +     D  GG    
Sbjct: 183 EFLEEFDVKSIPRFTALNINVPSVPYGELKGWKLTRQSKRMYE-DYFEQRKDPSGGNYYW 233

Query: 254 TMTMEPAASVAECYMTNESSELFTRQVGAPVDDEDTDFKYLREGYITVTPLA 304
            M      ++ E    N+              D   D+K + E Y++VTP++
Sbjct: 243 MM-----GNIIE----ND-------------PDPKADYKAVAEKYVSVTPIS 233

BLAST of MC05g0527 vs. ExPASy Swiss-Prot
Match: B7IH68 (5'-nucleotidase SurE OS=Thermosipho africanus (strain TCF52B) OX=484019 GN=surE PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.8e-25
Identity = 91/294 (30.95%), Postives = 139/294 (47.28%), Query Frame = 0

Query: 14  IMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITWRHPVSVKKVAI-E 73
           I+VTNDDG+ A G+  L R L  ++ Y V V AP++E+SAV  +IT R P+ ++K+ I E
Sbjct: 3   ILVTNDDGVTADGILCLARTL--SKKYKVTVVAPETEQSAVGHAITLRLPLWLRKLDINE 62

Query: 74  GTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVTNLDRFALILNYF 133
               Y+VSGTPADC  +GI   L    PDL++SGIN G+N G                  
Sbjct: 63  NFEIYSVSGTPADCVKMGIDVVLGEK-PDLLISGINRGNNLG------------------ 122

Query: 134 DSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDYTLAAEACLPIIS 193
                    +++VYSGTV+GA E    G+PSI++        S+ +      E     I 
Sbjct: 123 ---------TDVVYSGTVSGALEGAIAGVPSIAV--------SSYSFENPMYETAAKFIL 182

Query: 194 AMLADAKAQTFPRSCFLNIDFPT-DIANHRGYKVTKQGRCMYKMGWRRVASDSQGGK--M 253
             L +   ++ PR   LNI+ P+      +G+K+T+Q + MY   + +    S G    M
Sbjct: 183 DFLEEFDVRSIPRFTALNINVPSVPYDQIKGWKLTRQSKRMYDDYFEKRVDPSGGNYYWM 233

Query: 254 LSTMTMEPAASVAECYMTNESSELFTRQVGAPVDDEDTDFKYLREGYITVTPLA 304
           + T+  +                           D   D+K + E Y++VTP++
Sbjct: 243 MGTIIEDD-------------------------PDPKADYKAIAENYVSVTPIS 233

BLAST of MC05g0527 vs. ExPASy Swiss-Prot
Match: B8DN39 (5'-nucleotidase SurE OS=Desulfovibrio vulgaris (strain DSM 19637 / Miyazaki F) OX=883 GN=surE PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.2e-24
Identity = 90/290 (31.03%), Postives = 133/290 (45.86%), Query Frame = 0

Query: 14  IMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITWRHPVSVKKVAIEG 73
           + +TNDDGI APGLRA+ + L+    + V V AP +E+SAV  ++T   P+ VK+    G
Sbjct: 3   VALTNDDGIQAPGLRAMYKALLDAG-HEVHVVAPVTEQSAVGHAVTISLPLRVKEFHENG 62

Query: 74  TTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVTNLDRFALILNYFD 133
                V GTP DC  LG+S  L    PD+VVSGIN G+N G                   
Sbjct: 63  FRGRGVYGTPTDCVKLGLS-CLLDKKPDVVVSGINAGANVG------------------- 122

Query: 134 SLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDYTLAAEACLPIISA 193
                    +I+YSGTV+ A EA   G P++++SY+      +     L+ +A     + 
Sbjct: 123 --------PDILYSGTVSAATEAAHMGYPALAVSYD------SFRPADLSGQAA--HAAG 182

Query: 194 MLADAKAQTFPRSCFLNIDFP-TDIANHRGYKVTKQGRCMYKMGWRRVASDSQGGKMLST 253
           +LA    Q  P  C +N+++P   +   +G +   Q R ++K  W    +D +GG     
Sbjct: 183 LLATLDWQALPARCVVNLNYPAVPMPEVKGVRACPQTRAVWK-DWYDHRTDPRGGSYWWL 232

Query: 254 MTMEPAASVAECYMTNESSELFTRQVGAPVDDEDTDFKYLREGYITVTPL 303
             + P  +VA                        TD   L EG+IT+TPL
Sbjct: 243 NGVIPPETVA----------------------PGTDRALLTEGWITLTPL 232

BLAST of MC05g0527 vs. NCBI nr
Match: XP_022138161.1 (uncharacterized protein LOC111009400 [Momordica charantia] >XP_022138162.1 uncharacterized protein LOC111009400 [Momordica charantia])

HSP 1 Score: 596 bits (1536), Expect = 4.67e-214
Identity = 305/333 (91.59%), Postives = 305/333 (91.59%), Query Frame = 0

Query: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60
           MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW
Sbjct: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60

Query: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 120
           RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHV   
Sbjct: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHV--- 120

Query: 121 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180
                                   VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY
Sbjct: 121 ------------------------VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180

Query: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240
           TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV
Sbjct: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240

Query: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQV-GAPVDDEDTDFKYLREGYITV 300
           ASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQV GAPVDDEDTDFKYLREGYITV
Sbjct: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQVIGAPVDDEDTDFKYLREGYITV 300

Query: 301 TPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 332
           TPLAALSRADIECQNFLEAWLPGVAAQPSPSAL
Sbjct: 301 TPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 306

BLAST of MC05g0527 vs. NCBI nr
Match: XP_038876371.1 (5'-nucleotidase SurE [Benincasa hispida])

HSP 1 Score: 532 bits (1371), Expect = 5.99e-189
Identity = 273/333 (81.98%), Postives = 289/333 (86.79%), Query Frame = 0

Query: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60
           MEA+ + SDSRPTIMVTNDDGIDAPGLR+LVRVL+STQLYNVQVCAPDSEKSAVSQSITW
Sbjct: 1   MEASTIDSDSRPTIMVTNDDGIDAPGLRSLVRVLISTQLYNVQVCAPDSEKSAVSQSITW 60

Query: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 120
            HPVSVK+VAIEGTTSYAVSGTPADC SLGISKALFP+IPD+VVSGINMGSNCGYHV   
Sbjct: 61  LHPVSVKRVAIEGTTSYAVSGTPADCASLGISKALFPTIPDMVVSGINMGSNCGYHV--- 120

Query: 121 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180
                                   VYSGTVAGAREAFFNGIPSISLSYEWVGGRS+I DY
Sbjct: 121 ------------------------VYSGTVAGAREAFFNGIPSISLSYEWVGGRSSIEDY 180

Query: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240
           TLAAEACLPIISAMLAD KAQTFPRSCFLNIDFPTDIA+HRGYK+TKQGR +YKMGWRRV
Sbjct: 181 TLAAEACLPIISAMLADVKAQTFPRSCFLNIDFPTDIASHRGYKLTKQGRSIYKMGWRRV 240

Query: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQV-GAPVDDEDTDFKYLREGYITV 300
            SDSQGGKMLSTMTME A+SV EC M+NESSELF RQV GAPVDDEDTDFKYLREGYITV
Sbjct: 241 TSDSQGGKMLSTMTMEAASSV-ECNMSNESSELFARQVIGAPVDDEDTDFKYLREGYITV 300

Query: 301 TPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 332
           TPLAALSRA+  C+ FLEAWLPGV A+PSPSAL
Sbjct: 301 TPLAALSRAE-NCEKFLEAWLPGVVARPSPSAL 304

BLAST of MC05g0527 vs. NCBI nr
Match: XP_004134138.1 (uncharacterized protein LOC101206491 isoform X1 [Cucumis sativus] >XP_011650981.1 uncharacterized protein LOC101206491 isoform X1 [Cucumis sativus] >XP_031739232.1 uncharacterized protein LOC101206491 isoform X1 [Cucumis sativus] >KGN56987.1 hypothetical protein Csa_010983 [Cucumis sativus])

HSP 1 Score: 530 bits (1366), Expect = 3.86e-188
Identity = 268/335 (80.00%), Postives = 290/335 (86.57%), Query Frame = 0

Query: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60
           MEAT V SDSRPTIMVTNDDGIDAPGLR+LVRVLVSTQLYNVQVCAPDSEKSAVSQSITW
Sbjct: 1   MEATTVHSDSRPTIMVTNDDGIDAPGLRSLVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60

Query: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 120
           RHPVSVK+VAIEGTTSYAVSGTPADC+SLG+SKALFP++PD+VVSGINMGSNCGYHV   
Sbjct: 61  RHPVSVKRVAIEGTTSYAVSGTPADCSSLGVSKALFPTVPDMVVSGINMGSNCGYHV--- 120

Query: 121 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180
                                   VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNI DY
Sbjct: 121 ------------------------VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNIEDY 180

Query: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240
           TLAA+ACLPIISAMLAD KAQ FPR+CFLNIDFPTDIANHRGYK+TKQGRC+Y MGWRRV
Sbjct: 181 TLAAQACLPIISAMLADVKAQNFPRNCFLNIDFPTDIANHRGYKLTKQGRCIYTMGWRRV 240

Query: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESS--ELFTRQV-GAPVDDEDTDFKYLREGYI 300
            SDSQGGKMLSTMTM+P +S+ EC M+ ESS  ELFTRQV  AP+D+EDTD+KYL EGYI
Sbjct: 241 TSDSQGGKMLSTMTMDPTSSM-ECKMSEESSSSELFTRQVISAPIDNEDTDYKYLLEGYI 300

Query: 301 TVTPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 332
           TVTPLAALSRA+ +C+NFLEAWLPGV A+PSPSAL
Sbjct: 301 TVTPLAALSRAETDCENFLEAWLPGVVARPSPSAL 307

BLAST of MC05g0527 vs. NCBI nr
Match: XP_008438680.1 (PREDICTED: 5'-nucleotidase SurE [Cucumis melo] >XP_008438681.1 PREDICTED: 5'-nucleotidase SurE [Cucumis melo])

HSP 1 Score: 526 bits (1355), Expect = 1.83e-186
Identity = 268/335 (80.00%), Postives = 288/335 (85.97%), Query Frame = 0

Query: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60
           MEAT V SDSRPTIMVTNDDGIDAPGLR+LVRVLVS+QLYNV+VCAPDSEKSAVSQSITW
Sbjct: 1   MEATTVHSDSRPTIMVTNDDGIDAPGLRSLVRVLVSSQLYNVRVCAPDSEKSAVSQSITW 60

Query: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 120
            HPVSVK+VAIEGTTSYAVSGTPADC+SLG+SKALFP++PD+VVSGINMGSNCGYHV   
Sbjct: 61  LHPVSVKRVAIEGTTSYAVSGTPADCSSLGVSKALFPTVPDMVVSGINMGSNCGYHV--- 120

Query: 121 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180
                                   VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNI DY
Sbjct: 121 ------------------------VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNIEDY 180

Query: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240
           TLAAEACLPIISAMLAD KAQ FPR+CFLNIDFPTDIANHRGYK+TKQGRC+Y MGWRRV
Sbjct: 181 TLAAEACLPIISAMLADVKAQIFPRNCFLNIDFPTDIANHRGYKLTKQGRCIYTMGWRRV 240

Query: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESS--ELFTRQV-GAPVDDEDTDFKYLREGYI 300
            SDSQGGKMLSTMTM+P + V EC M+ ESS  ELFTRQV  APVD+EDTD+KYL EGYI
Sbjct: 241 TSDSQGGKMLSTMTMDPTSPV-ECKMSKESSSSELFTRQVISAPVDNEDTDYKYLLEGYI 300

Query: 301 TVTPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 332
           TVTPLAALSRA+ EC+NFLEAWLPGV A+PSPSAL
Sbjct: 301 TVTPLAALSRAETECENFLEAWLPGVVARPSPSAL 307

BLAST of MC05g0527 vs. NCBI nr
Match: XP_023538688.1 (uncharacterized protein LOC111799559 [Cucurbita pepo subsp. pepo] >XP_023538689.1 uncharacterized protein LOC111799559 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 522 bits (1344), Expect = 7.76e-185
Identity = 266/333 (79.88%), Postives = 284/333 (85.29%), Query Frame = 0

Query: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60
           MEAT V SDSRPTIM+TNDDGIDAPG+RALVRVLVST+LYNVQVCAPDSEKSAVSQSITW
Sbjct: 1   MEATIVDSDSRPTIMITNDDGIDAPGIRALVRVLVSTKLYNVQVCAPDSEKSAVSQSITW 60

Query: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 120
           RHP+S K+V IEGTTSYAVSGTPADCTSLGIS  LFPS PDLVVSGINMGSNCGYHV   
Sbjct: 61  RHPISAKRVDIEGTTSYAVSGTPADCTSLGISGTLFPSKPDLVVSGINMGSNCGYHV--- 120

Query: 121 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180
                                   VYSGTVAGAREAFFNGIPS+SLSY+WVGGRSNINDY
Sbjct: 121 ------------------------VYSGTVAGAREAFFNGIPSVSLSYDWVGGRSNINDY 180

Query: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240
           TLAAEACLPIISAMLAD KAQTFP++CFLNIDFPTDIANHRGYK+TKQGRCMYK+GWRRV
Sbjct: 181 TLAAEACLPIISAMLADVKAQTFPQNCFLNIDFPTDIANHRGYKLTKQGRCMYKLGWRRV 240

Query: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQV-GAPVDDEDTDFKYLREGYITV 300
            SDS GGKMLSTMTMEPA+SV EC  +NES ELFTRQV   PVDDEDTD+KYLREGYITV
Sbjct: 241 TSDSPGGKMLSTMTMEPASSV-ECNTSNES-ELFTRQVISPPVDDEDTDYKYLREGYITV 300

Query: 301 TPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 332
           TPLAALSRA+++C+N LEAWL GV A PS SAL
Sbjct: 301 TPLAALSRAEVDCENILEAWLLGVVAPPSASAL 304

BLAST of MC05g0527 vs. ExPASy TrEMBL
Match: A0A6J1CAA9 (uncharacterized protein LOC111009400 OS=Momordica charantia OX=3673 GN=LOC111009400 PE=3 SV=1)

HSP 1 Score: 596 bits (1536), Expect = 2.26e-214
Identity = 305/333 (91.59%), Postives = 305/333 (91.59%), Query Frame = 0

Query: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60
           MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW
Sbjct: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60

Query: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 120
           RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHV   
Sbjct: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHV--- 120

Query: 121 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180
                                   VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY
Sbjct: 121 ------------------------VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180

Query: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240
           TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV
Sbjct: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240

Query: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQV-GAPVDDEDTDFKYLREGYITV 300
           ASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQV GAPVDDEDTDFKYLREGYITV
Sbjct: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQVIGAPVDDEDTDFKYLREGYITV 300

Query: 301 TPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 332
           TPLAALSRADIECQNFLEAWLPGVAAQPSPSAL
Sbjct: 301 TPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 306

BLAST of MC05g0527 vs. ExPASy TrEMBL
Match: A0A0A0L8Q7 (SurE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G147210 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 1.87e-188
Identity = 268/335 (80.00%), Postives = 290/335 (86.57%), Query Frame = 0

Query: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60
           MEAT V SDSRPTIMVTNDDGIDAPGLR+LVRVLVSTQLYNVQVCAPDSEKSAVSQSITW
Sbjct: 1   MEATTVHSDSRPTIMVTNDDGIDAPGLRSLVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60

Query: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 120
           RHPVSVK+VAIEGTTSYAVSGTPADC+SLG+SKALFP++PD+VVSGINMGSNCGYHV   
Sbjct: 61  RHPVSVKRVAIEGTTSYAVSGTPADCSSLGVSKALFPTVPDMVVSGINMGSNCGYHV--- 120

Query: 121 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180
                                   VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNI DY
Sbjct: 121 ------------------------VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNIEDY 180

Query: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240
           TLAA+ACLPIISAMLAD KAQ FPR+CFLNIDFPTDIANHRGYK+TKQGRC+Y MGWRRV
Sbjct: 181 TLAAQACLPIISAMLADVKAQNFPRNCFLNIDFPTDIANHRGYKLTKQGRCIYTMGWRRV 240

Query: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESS--ELFTRQV-GAPVDDEDTDFKYLREGYI 300
            SDSQGGKMLSTMTM+P +S+ EC M+ ESS  ELFTRQV  AP+D+EDTD+KYL EGYI
Sbjct: 241 TSDSQGGKMLSTMTMDPTSSM-ECKMSEESSSSELFTRQVISAPIDNEDTDYKYLLEGYI 300

Query: 301 TVTPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 332
           TVTPLAALSRA+ +C+NFLEAWLPGV A+PSPSAL
Sbjct: 301 TVTPLAALSRAETDCENFLEAWLPGVVARPSPSAL 307

BLAST of MC05g0527 vs. ExPASy TrEMBL
Match: A0A1S3AX01 (5'-nucleotidase SurE OS=Cucumis melo OX=3656 GN=LOC103483713 PE=3 SV=1)

HSP 1 Score: 526 bits (1355), Expect = 8.86e-187
Identity = 268/335 (80.00%), Postives = 288/335 (85.97%), Query Frame = 0

Query: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60
           MEAT V SDSRPTIMVTNDDGIDAPGLR+LVRVLVS+QLYNV+VCAPDSEKSAVSQSITW
Sbjct: 1   MEATTVHSDSRPTIMVTNDDGIDAPGLRSLVRVLVSSQLYNVRVCAPDSEKSAVSQSITW 60

Query: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 120
            HPVSVK+VAIEGTTSYAVSGTPADC+SLG+SKALFP++PD+VVSGINMGSNCGYHV   
Sbjct: 61  LHPVSVKRVAIEGTTSYAVSGTPADCSSLGVSKALFPTVPDMVVSGINMGSNCGYHV--- 120

Query: 121 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180
                                   VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNI DY
Sbjct: 121 ------------------------VYSGTVAGAREAFFNGIPSISLSYEWVGGRSNIEDY 180

Query: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240
           TLAAEACLPIISAMLAD KAQ FPR+CFLNIDFPTDIANHRGYK+TKQGRC+Y MGWRRV
Sbjct: 181 TLAAEACLPIISAMLADVKAQIFPRNCFLNIDFPTDIANHRGYKLTKQGRCIYTMGWRRV 240

Query: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESS--ELFTRQV-GAPVDDEDTDFKYLREGYI 300
            SDSQGGKMLSTMTM+P + V EC M+ ESS  ELFTRQV  APVD+EDTD+KYL EGYI
Sbjct: 241 TSDSQGGKMLSTMTMDPTSPV-ECKMSKESSSSELFTRQVISAPVDNEDTDYKYLLEGYI 300

Query: 301 TVTPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 332
           TVTPLAALSRA+ EC+NFLEAWLPGV A+PSPSAL
Sbjct: 301 TVTPLAALSRAETECENFLEAWLPGVVARPSPSAL 307

BLAST of MC05g0527 vs. ExPASy TrEMBL
Match: A0A6J1GVL0 (uncharacterized protein LOC111457877 OS=Cucurbita moschata OX=3662 GN=LOC111457877 PE=3 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 3.90e-185
Identity = 266/333 (79.88%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60
           ME + V SDSRPTIM+TNDDGIDAPGLRALVRVLVSTQLYNV VCAPDSEKSAVSQSITW
Sbjct: 1   MEVSTVDSDSRPTIMITNDDGIDAPGLRALVRVLVSTQLYNVLVCAPDSEKSAVSQSITW 60

Query: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 120
           RHP+SVK+VAIEGTTSYAVSGTPADCTSLG+SKALFPSIPDLV+SGINMGSNCGYHV   
Sbjct: 61  RHPISVKRVAIEGTTSYAVSGTPADCTSLGVSKALFPSIPDLVLSGINMGSNCGYHV--- 120

Query: 121 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180
                                   VYSGTVAGAREAFF GIPS SLSYEW+GGRSNI DY
Sbjct: 121 ------------------------VYSGTVAGAREAFFKGIPSASLSYEWIGGRSNIEDY 180

Query: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240
           TLAAEACLPIISAML   KAQTFPR+CFLNIDFPTDIANHRGYKVTKQGRC+ KMGW RV
Sbjct: 181 TLAAEACLPIISAMLVGVKAQTFPRNCFLNIDFPTDIANHRGYKVTKQGRCISKMGWIRV 240

Query: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQV-GAPVDDEDTDFKYLREGYITV 300
            SDSQGGKMLSTMTM+P +SV EC M++ESSELF RQV  A V DEDTDFKYL+EGYITV
Sbjct: 241 TSDSQGGKMLSTMTMDPTSSV-ECNMSSESSELFARQVISAAVADEDTDFKYLQEGYITV 300

Query: 301 TPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 332
           TPLAALSRA+IEC+ FLEAWLPGV A+PSPSAL
Sbjct: 301 TPLAALSRAEIECEIFLEAWLPGVVARPSPSAL 305

BLAST of MC05g0527 vs. ExPASy TrEMBL
Match: A0A6J1IQH6 (uncharacterized protein LOC111479092 OS=Cucurbita maxima OX=3661 GN=LOC111479092 PE=3 SV=1)

HSP 1 Score: 521 bits (1341), Expect = 1.12e-184
Identity = 266/333 (79.88%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 1   MEATAVSSDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITW 60
           ME + V SDSR TIM+TNDDGIDAPGLRALVRVLVSTQLYNV VCAPDSEKSAVSQSITW
Sbjct: 1   MEVSTVDSDSRLTIMITNDDGIDAPGLRALVRVLVSTQLYNVLVCAPDSEKSAVSQSITW 60

Query: 61  RHPVSVKKVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVT 120
           RHP+SVK+VAIEGTTSYAVSGTPADCTSLG+SKALFPSIPDLV+SGINMGSNCGYHV   
Sbjct: 61  RHPISVKRVAIEGTTSYAVSGTPADCTSLGVSKALFPSIPDLVLSGINMGSNCGYHV--- 120

Query: 121 NLDRFALILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDY 180
                                   VYSGTVAGAREAFF GIPS SLSYEWVGGRSNI DY
Sbjct: 121 ------------------------VYSGTVAGAREAFFKGIPSASLSYEWVGGRSNIEDY 180

Query: 181 TLAAEACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRV 240
           TLAAEACLPIISAML   KAQTFPR+CFLNIDFPTDIANHRGYKVTKQGRC+ KMGW RV
Sbjct: 181 TLAAEACLPIISAMLVGVKAQTFPRNCFLNIDFPTDIANHRGYKVTKQGRCISKMGWIRV 240

Query: 241 ASDSQGGKMLSTMTMEPAASVAECYMTNESSELFTRQV-GAPVDDEDTDFKYLREGYITV 300
            SDSQGGKMLSTMTM+P +SV EC M++ESSELF RQV  A V DEDTDFKYL+EGYITV
Sbjct: 241 TSDSQGGKMLSTMTMDPTSSV-ECNMSSESSELFARQVISAAVADEDTDFKYLQEGYITV 300

Query: 301 TPLAALSRADIECQNFLEAWLPGVAAQPSPSAL 332
           TPLAALSRA+I+C+NFLEAWLPGV A+PSPSAL
Sbjct: 301 TPLAALSRAEIDCENFLEAWLPGVVARPSPSAL 305

BLAST of MC05g0527 vs. TAIR 10
Match: AT4G14930.1 (Survival protein SurE-like phosphatase/nucleotidase )

HSP 1 Score: 362.1 bits (928), Expect = 4.9e-100
Identity = 184/331 (55.59%), Postives = 230/331 (69.49%), Query Frame = 0

Query: 8   SDSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITWRHPVSVK 67
           S  RP IMVTNDDGIDAPGLR+LVRVLVST LY+V+VCAPDSEKSAVS SI W  P++ K
Sbjct: 10  SGERPIIMVTNDDGIDAPGLRSLVRVLVSTNLYDVRVCAPDSEKSAVSHSIIWSRPLTAK 69

Query: 68  KVAIEGTTSYAVSGTPADCTSLGISKALFPSIPDLVVSGINMGSNCGYHVLVTNLDRFAL 127
           +V I+G T+Y+V GTPADCT LG+S+ALFPS PDLV+SGIN+GSNCGY            
Sbjct: 70  RVEIDGATAYSVDGTPADCTGLGLSEALFPSRPDLVLSGINVGSNCGY------------ 129

Query: 128 ILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSY--EWVGGRSNINDYTLAAE 187
                          NIVYSGTVAGAREAF   +PS S+SY  +W  G  N ND+ L+A+
Sbjct: 130 ---------------NIVYSGTVAGAREAFLYDVPSASISYDFDWKRGEMNANDFALSAQ 189

Query: 188 ACLPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRVASDSQ 247
           ACLPII+ +L   K +T P  CFLNID PTDIANH+GYK+T+QG+ M KMGWR+V  ++Q
Sbjct: 190 ACLPIINGILGAIKNKTHPMQCFLNIDLPTDIANHKGYKLTRQGKSMGKMGWRQVEEEAQ 249

Query: 248 GGKMLSTMTMEPAASVAE-----CYMTNESSELFTRQVGAPVDDEDTDFKYLREGYITVT 307
           G KMLSTMTM+  + V            + S LF R++ A + +E +D  YL+EG+ITVT
Sbjct: 250 GPKMLSTMTMDTESGVVSSENDTSAHAGKDSRLFKRELRASISEEGSDSHYLKEGFITVT 309

Query: 308 PLAALSRADIECQNFLEAWLPGVAAQPSPSA 332
           PL ALS+ D++CQN+ + WLP +  Q   S+
Sbjct: 310 PLGALSQTDVDCQNYYKEWLPKITNQSCSSS 313

BLAST of MC05g0527 vs. TAIR 10
Match: AT1G72880.2 (Survival protein SurE-like phosphatase/nucleotidase )

HSP 1 Score: 206.1 bits (523), Expect = 4.5e-53
Identity = 124/347 (35.73%), Postives = 174/347 (50.14%), Query Frame = 0

Query: 9   DSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITWRHPVSVKK 68
           DSRP ++VTN DGID+PGL +LV  LV   LYNV VCAP ++KSA + S T    ++V  
Sbjct: 58  DSRPIVLVTNGDGIDSPGLVSLVEALVLEGLYNVHVCAPQTDKSASAHSTTPGETIAVSS 117

Query: 69  VAIEGTTSYAVSGTPADCTSLGISKALFP-SIPDLVVSGINMGSNCGYHVLVTNLDRFAL 128
           V ++G T++ VSGTP DC SLG+S ALF  S P LV+SGIN GS+CG+ +          
Sbjct: 118 VKLKGATAFEVSGTPVDCISLGLSGALFAWSKPILVISGINQGSSCGHQMF--------- 177

Query: 129 ILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDYTLAAEAC 188
                             YSG VAG REA  +G+PS+S+S  W    S  + +  A   C
Sbjct: 178 ------------------YSGAVAGGREALISGVPSLSISLNWKKNESQESHFKDAVGVC 237

Query: 189 LPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRVASD---- 248
           LP+I+A + D     FP+ C LNI+ PT  ++++G+KVTKQ        W+ V+++    
Sbjct: 238 LPLINATIRDIAKGVFPKDCSLNIEIPTSPSSNKGFKVTKQSMWRQYPSWQAVSANRHPG 297

Query: 249 ---------SQGGKMLSTMTMEPAASVAECYMTNESSELFTRQVGA-------------- 308
                    S G ++        AA  A  + T + S +    VG               
Sbjct: 298 AGNFMSNQQSLGAQLAQLGRDASAAGAARRFTTQKKSIVEIESVGVAGKTDTRVKKFFRL 357

Query: 309 -------PVDDEDTDFKYLREGYITVTPLAALSRADIECQNFLEAWL 321
                     DED D K L +G+++VTP + L + D E Q     W+
Sbjct: 358 EFLAKEQEHTDEDLDVKALEDGFVSVTPFSLLPKTDSETQAAASEWI 377

BLAST of MC05g0527 vs. TAIR 10
Match: AT1G72880.1 (Survival protein SurE-like phosphatase/nucleotidase )

HSP 1 Score: 206.1 bits (523), Expect = 4.5e-53
Identity = 124/347 (35.73%), Postives = 174/347 (50.14%), Query Frame = 0

Query: 9   DSRPTIMVTNDDGIDAPGLRALVRVLVSTQLYNVQVCAPDSEKSAVSQSITWRHPVSVKK 68
           DSRP ++VTN DGID+PGL +LV  LV   LYNV VCAP ++KSA + S T    ++V  
Sbjct: 58  DSRPIVLVTNGDGIDSPGLVSLVEALVLEGLYNVHVCAPQTDKSASAHSTTPGETIAVSS 117

Query: 69  VAIEGTTSYAVSGTPADCTSLGISKALFP-SIPDLVVSGINMGSNCGYHVLVTNLDRFAL 128
           V ++G T++ VSGTP DC SLG+S ALF  S P LV+SGIN GS+CG+ +          
Sbjct: 118 VKLKGATAFEVSGTPVDCISLGLSGALFAWSKPILVISGINQGSSCGHQMF--------- 177

Query: 129 ILNYFDSLTINTEVSNIVYSGTVAGAREAFFNGIPSISLSYEWVGGRSNINDYTLAAEAC 188
                             YSG VAG REA  +G+PS+S+S  W    S  + +  A   C
Sbjct: 178 ------------------YSGAVAGGREALISGVPSLSISLNWKKNESQESHFKDAVGVC 237

Query: 189 LPIISAMLADAKAQTFPRSCFLNIDFPTDIANHRGYKVTKQGRCMYKMGWRRVASD---- 248
           LP+I+A + D     FP+ C LNI+ PT  ++++G+KVTKQ        W+ V+++    
Sbjct: 238 LPLINATIRDIAKGVFPKDCSLNIEIPTSPSSNKGFKVTKQSMWRQYPSWQAVSANRHPG 297

Query: 249 ---------SQGGKMLSTMTMEPAASVAECYMTNESSELFTRQVGA-------------- 308
                    S G ++        AA  A  + T + S +    VG               
Sbjct: 298 AGNFMSNQQSLGAQLAQLGRDASAAGAARRFTTQKKSIVEIESVGVAGKTDTRVKKFFRL 357

Query: 309 -------PVDDEDTDFKYLREGYITVTPLAALSRADIECQNFLEAWL 321
                     DED D K L +G+++VTP + L + D E Q     W+
Sbjct: 358 EFLAKEQEHTDEDLDVKALEDGFVSVTPFSLLPKTDSETQAAASEWI 377

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A6LS611.8e-2731.745'-nucleotidase SurE OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052... [more]
Q1J2E11.2e-2633.755'-nucleotidase SurE OS=Deinococcus geothermalis (strain DSM 11300 / AG-3a) OX=3... [more]
A6LL962.9e-2531.855'-nucleotidase SurE OS=Thermosipho melanesiensis (strain DSM 12029 / CIP 104789... [more]
B7IH683.8e-2530.955'-nucleotidase SurE OS=Thermosipho africanus (strain TCF52B) OX=484019 GN=surE ... [more]
B8DN394.2e-2431.035'-nucleotidase SurE OS=Desulfovibrio vulgaris (strain DSM 19637 / Miyazaki F) O... [more]
Match NameE-valueIdentityDescription
XP_022138161.14.67e-21491.59uncharacterized protein LOC111009400 [Momordica charantia] >XP_022138162.1 uncha... [more]
XP_038876371.15.99e-18981.985'-nucleotidase SurE [Benincasa hispida][more]
XP_004134138.13.86e-18880.00uncharacterized protein LOC101206491 isoform X1 [Cucumis sativus] >XP_011650981.... [more]
XP_008438680.11.83e-18680.00PREDICTED: 5'-nucleotidase SurE [Cucumis melo] >XP_008438681.1 PREDICTED: 5'-nuc... [more]
XP_023538688.17.76e-18579.88uncharacterized protein LOC111799559 [Cucurbita pepo subsp. pepo] >XP_023538689.... [more]
Match NameE-valueIdentityDescription
A0A6J1CAA92.26e-21491.59uncharacterized protein LOC111009400 OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A0A0L8Q71.87e-18880.00SurE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G147210 PE=3 S... [more]
A0A1S3AX018.86e-18780.005'-nucleotidase SurE OS=Cucumis melo OX=3656 GN=LOC103483713 PE=3 SV=1[more]
A0A6J1GVL03.90e-18579.88uncharacterized protein LOC111457877 OS=Cucurbita moschata OX=3662 GN=LOC1114578... [more]
A0A6J1IQH61.12e-18479.88uncharacterized protein LOC111479092 OS=Cucurbita maxima OX=3661 GN=LOC111479092... [more]
Match NameE-valueIdentityDescription
AT4G14930.14.9e-10055.59Survival protein SurE-like phosphatase/nucleotidase [more]
AT1G72880.24.5e-5335.73Survival protein SurE-like phosphatase/nucleotidase [more]
AT1G72880.14.5e-5335.73Survival protein SurE-like phosphatase/nucleotidase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002828Survival protein SurE-like phosphatase/nucleotidasePFAMPF01975SurEcoord: 143..234
e-value: 4.2E-14
score: 52.8
coord: 14..118
e-value: 4.1E-33
score: 114.8
IPR036523SurE-like phosphatase/nucleotidase superfamilyGENE3D3.40.1210.10coord: 13..120
e-value: 2.4E-38
score: 134.0
IPR036523SurE-like phosphatase/nucleotidase superfamilyGENE3D3.40.1210.10coord: 137..325
e-value: 1.6E-27
score: 98.4
IPR036523SurE-like phosphatase/nucleotidase superfamilySUPERFAMILY64167SurE-likecoord: 138..319
IPR036523SurE-like phosphatase/nucleotidase superfamilySUPERFAMILY64167SurE-likecoord: 13..117
IPR030048Survival protein SurEPANTHERPTHR304575'-NUCLEOTIDASE SUREcoord: 1..118
coord: 143..331
IPR030048Survival protein SurEHAMAPMF_00060SurEcoord: 12..325
score: 27.813747
NoneNo IPR availablePANTHERPTHR30457:SF0OS07G0204500 PROTEINcoord: 1..118
coord: 143..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0527.1MC05g0527.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008252 nucleotidase activity
molecular_function GO:0016787 hydrolase activity