MC05g0497 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0497
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionstem-specific protein TSJT1
LocationMC05: 3724727 .. 3727053 (-)
RNA-Seq ExpressionMC05g0497
SyntenyMC05g0497
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAACTAAAAGCATCTTATATAGATATAAAGCGATGCTAAAAGTAGGAAAGAAATAGAATAAATGCTTTTGTCATTTTTTTCTTAAAAAATTAGTCTGATGTCTTCATAAATCTGTGGGTGGCCTGGTATGTAAATTATGAAAATTAGATAAAAGAAGAGGGGCAATAGTGGAAATTTGACTGACCTTATTATTTTTATTTATTTTGAAGTATCTCCTCCTCCAACCGCATGCGATCTTTCATCAGCTGCTATTTCTGCGTTCACAAAGGGAAGAAGCAAAATCAAGCGTGTGTCTGAGTTCTCTCATTTTCCGATCGACAATATATTGAAAATTTTAAAATAAAAAAAAAAAAATGCTCGCCGTGTTCGACAAATCGGTGGCGAAGACTCCCGAAGCTCTGCAGAGCCCGCACTCCGATTCCGTTTGGGCTCTCAAAGATGGGATTCTCGGTCGGCATTTCGGCTCTCTCTACCCTGCCTCCGTCATCGTCAACCTCGGCTCCGCCGGTCTCTTGGCCTACTCCGTTGAAAAGCAAAACCCTCTCCTCCCGAGGTACTCCGCTTTTGTTCTCTCTTATACTGCTATGTCTCTTTCGACTAAAATTTGCTGTGTTTGATTTGTTCTGGATCTGATCGACGATCGTGCTTACTTACTGTCTACTGATCGTCTCTTCTATCGGGTGTTTCGTTCTGATTGCTGAGAATCTGTTTAAATTCAACAGAATCATATTTTCTGTTTTGTGGAGATTAGGTTTTGATGGGAAATGCTTGTACTCTTTGAGGCCATTCTGCCATTTTTTTTTCTCGAGTTTTTCCATGTTGACTCGATCGGAACTGTGGACGATCAAATCTTTCGAGATCGACGATTTAGTACTCGTCTGATTCTAAATGATTGTACTCTTGTTTTATATGATCAACTTAAATTGTGAGATTTGTGTTGAATCATTGGTAAGAAAGCTGGGGATTTTACGGCTGCAATTTTTGGTAGCTCTATTTCATGAGCATTTGGTTATATTTGCCTGCTGTTGGTAAATTGACGAACTTTGCCTTTATGACCTTAGATTATTTGCGGTTGTGGACGACATTTTCTGCTTGTTCCAAGGGCACATTGAGAATGTTGCGCAGCTGAAGCAACAATATGGTTTGAACAAAGCTGCAAACGAGGTTATCATCGTTATAGAAGCCTATCGAACATTAAGAGATCGGGGTCCATACCCTGCTGATCAGGTTGTGAGAGACATTCAAGGGAAATTCGTGTTTGTTCTATACGACAGTTCCTCAAAAACCGCATTTTTCGCTTCTGTAAGTTGCCCTGATCCCTTAGGTTAACCATGACTGCTGCAGATTTCTGTTATTGTTGTTTATGTTAGCTTTTTTTTTCCATTATGGTGTCTTTTCTTTTTATCTGCAGGATGCCGATGGGAGCGTTCCTCTCCACTGGGGAAGTGATTCTGAAGGACATCTTGTTCTCTCAGATGATATAGAAACTATGAAGAAGGGTTGTGGAAAATCTTTTGCTCCTTTCCCCAAAGGTAAAACTTCTAATCGAATTGCTATGTCACGAATAAAAAGTTTTGCTCCCTTTCTAATTCGTTGTCTTCAAATTTGAGAATTGGTTCATTGATGTTATAGCTTGCATACCTTGTGGGATTGCCTGTTTCTGAAATAAGTAAAATACAACTAGTTCTAGATACTTGTTTCTTTGGTTTGTTCATTTATATTTCTGCACTACGTTTGGTGCTTTAGGTTGCTTCTTCACAACCTCGGGTGGGTTGAGGAGTTTTGAGCATCCACTTAATGAGCTGAAGCCAGTGCCAAGGGTGGACAGTTCAGGTCATGTTTGTGGAGCCAATTTCAAGGTGGATGAAGAGGCTCGAAAGGAAAGTTCTGGCATGCCTAGAGTTGGGAGTGCTGCAAATTGGTCTTCAAACTACTGAGTGCACAATTTGGCAAGGCTTTGATTACTTTGCCTTTGCCTTTCCTTCGGCTTCTTTCCATATTTTATTCCCTGACTGTGCCCTTGAAAATGGGTACTGAGGTTTATACTTTACTTTCCGTAATGGGCATGTTCATGCAGTTATAACTTACAAGTTATGAATCTTGCTGGCTCCACTGAATTACATTCTGGATACGTGGAATAATAGTTTGTTTCTAAATGCTTTTAGACATATTTTTATTTGCTTTTGGGGTTGCCTCTCTGTCATGACCTTTTCTAGAAATTGGCTCTATTTGTGTTCATAATGGTTGAAGGGCATTGATCGTAACAGAATTTTGCTCTAGTTTTGGGTTTATGCCAATAAAGTGGCTCCTTTCCAACGG

mRNA sequence

AAAAAAAAAAACTAAAAGCATCTTATATAGATATAAAGCGATGCTAAAAGTAGGAAAGAAATAGAATAAATGCTTTTGTCATTTTTTTCTTAAAAAATTAGTCTGATGTCTTCATAAATCTGTGGGTGGCCTGTATCTCCTCCTCCAACCGCATGCGATCTTTCATCAGCTGCTATTTCTGCGTTCACAAAGGGAAGAAGCAAAATCAAGCGTGTGTCTGAGTTCTCTCATTTTCCGATCGACAATATATTGAAAATTTTAAAATAAAAAAAAAAAAATGCTCGCCGTGTTCGACAAATCGGTGGCGAAGACTCCCGAAGCTCTGCAGAGCCCGCACTCCGATTCCGTTTGGGCTCTCAAAGATGGGATTCTCGGTCGGCATTTCGGCTCTCTCTACCCTGCCTCCGTCATCGTCAACCTCGGCTCCGCCGGTCTCTTGGCCTACTCCGTTGAAAAGCAAAACCCTCTCCTCCCGAGATTATTTGCGGTTGTGGACGACATTTTCTGCTTGTTCCAAGGGCACATTGAGAATGTTGCGCAGCTGAAGCAACAATATGGTTTGAACAAAGCTGCAAACGAGGTTATCATCGTTATAGAAGCCTATCGAACATTAAGAGATCGGGGTCCATACCCTGCTGATCAGGTTGTGAGAGACATTCAAGGGAAATTCGTGTTTGTTCTATACGACAGTTCCTCAAAAACCGCATTTTTCGCTTCTGATGCCGATGGGAGCGTTCCTCTCCACTGGGGAAGTGATTCTGAAGGACATCTTGTTCTCTCAGATGATATAGAAACTATGAAGAAGGGTTGTGGAAAATCTTTTGCTCCTTTCCCCAAAGGTTGCTTCTTCACAACCTCGGGTGGGTTGAGGAGTTTTGAGCATCCACTTAATGAGCTGAAGCCAGTGCCAAGGGTGGACAGTTCAGGTCATGTTTGTGGAGCCAATTTCAAGGTGGATGAAGAGGCTCGAAAGGAAAGTTCTGGCATGCCTAGAGTTGGGAGTGCTGCAAATTGGTCTTCAAACTACTGAGTGCACAATTTGGCAAGGCTTTGATTACTTTGCCTTTGCCTTTCCTTCGGCTTCTTTCCATATTTTATTCCCTGACTGTGCCCTTGAAAATGGGTACTGAGGTTTATACTTTACTTTCCGTAATGGGCATGTTCATGCAGTTATAACTTACAAGTTATGAATCTTGCTGGCTCCACTGAATTACATTCTGGATACGTGGAATAATAGTTTGTTTCTAAATGCTTTTAGACATATTTTTATTTGCTTTTGGGGTTGCCTCTCTGTCATGACCTTTTCTAGAAATTGGCTCTATTTGTGTTCATAATGGTTGAAGGGCATTGATCGTAACAGAATTTTGCTCTAGTTTTGGGTTTATGCCAATAAAGTGGCTCCTTTCCAACGG

Coding sequence (CDS)

ATGCTCGCCGTGTTCGACAAATCGGTGGCGAAGACTCCCGAAGCTCTGCAGAGCCCGCACTCCGATTCCGTTTGGGCTCTCAAAGATGGGATTCTCGGTCGGCATTTCGGCTCTCTCTACCCTGCCTCCGTCATCGTCAACCTCGGCTCCGCCGGTCTCTTGGCCTACTCCGTTGAAAAGCAAAACCCTCTCCTCCCGAGATTATTTGCGGTTGTGGACGACATTTTCTGCTTGTTCCAAGGGCACATTGAGAATGTTGCGCAGCTGAAGCAACAATATGGTTTGAACAAAGCTGCAAACGAGGTTATCATCGTTATAGAAGCCTATCGAACATTAAGAGATCGGGGTCCATACCCTGCTGATCAGGTTGTGAGAGACATTCAAGGGAAATTCGTGTTTGTTCTATACGACAGTTCCTCAAAAACCGCATTTTTCGCTTCTGATGCCGATGGGAGCGTTCCTCTCCACTGGGGAAGTGATTCTGAAGGACATCTTGTTCTCTCAGATGATATAGAAACTATGAAGAAGGGTTGTGGAAAATCTTTTGCTCCTTTCCCCAAAGGTTGCTTCTTCACAACCTCGGGTGGGTTGAGGAGTTTTGAGCATCCACTTAATGAGCTGAAGCCAGTGCCAAGGGTGGACAGTTCAGGTCATGTTTGTGGAGCCAATTTCAAGGTGGATGAAGAGGCTCGAAAGGAAAGTTCTGGCATGCCTAGAGTTGGGAGTGCTGCAAATTGGTCTTCAAACTACTGA

Protein sequence

MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEKQNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPADQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGKSFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRVGSAANWSSNY
Homology
BLAST of MC05g0497 vs. ExPASy Swiss-Prot
Match: P24805 (Stem-specific protein TSJT1 OS=Nicotiana tabacum OX=4097 GN=TSJT1 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.8e-28
Identity = 58/148 (39.19%), Postives = 91/148 (61.49%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHS--DSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSV 60
           MLAVF++S+ + P  L  P +      A     +   F +    S   +L +   +A+S 
Sbjct: 1   MLAVFEQSIGRPPPELSLPQAGIQKKEAKTREEIAESFKTWKQDSTFYHLFNGNFMAFSH 60

Query: 61  EKQNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPY 120
             +NPL PR   V+DD+FC+F G ++N   L++ YGL++ A E +I++EAY+ LRDR PY
Sbjct: 61  GNENPLQPRSIVVMDDVFCIFSGALDNTFDLRKHYGLSRQATEAMIMVEAYKVLRDRAPY 120

Query: 121 PADQVVRDIQGKFVFVLYDSSSKTAFFA 147
           P DQV+++++GKF F+L+DS + T F A
Sbjct: 121 PPDQVIKELEGKFAFILFDSKASTLFLA 148

BLAST of MC05g0497 vs. ExPASy Swiss-Prot
Match: P22106 (Asparagine synthetase B [glutamine-hydrolyzing] OS=Escherichia coli (strain K12) OX=83333 GN=asnB PE=1 SV=3)

HSP 1 Score: 58.5 bits (140), Expect = 1.2e-07
Identity = 34/126 (26.98%), Postives = 63/126 (50.00%), Query Frame = 0

Query: 81  GHIENVAQLKQQYGLN---KAANEVIIVIEAYRTLRDRGPYPADQVVRDIQGKFVFVLYD 140
           G I N   L+ +YG     +  ++  +++  Y   +++GP    + + D+QG F F LYD
Sbjct: 76  GEIYNHQALRAEYGDRYQFQTGSDCEVILALY---QEKGP----EFLDDLQGMFAFALYD 135

Query: 141 SSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGKSFAPFPKGCF-FTTSGG 200
           S         D  G +PL+ G D  G L ++ +++ +   C ++   FP G + ++  G 
Sbjct: 136 SEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKALVPVC-RTIKEFPAGSYLWSQDGE 193

Query: 201 LRSFEH 203
           +RS+ H
Sbjct: 196 IRSYYH 193

BLAST of MC05g0497 vs. ExPASy Swiss-Prot
Match: Q10MX3 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0291500 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 7.9e-07
Identity = 37/133 (27.82%), Postives = 65/133 (48.87%), Query Frame = 0

Query: 75  IFCLFQGHIENVAQLKQQYGLN---KAANEVIIVIEAYRTLRDRGPYPADQVVRDIQGKF 134
           I  +  G I N  ++++Q+         ++  ++I  Y           +  V  + G F
Sbjct: 70  IVVVANGEIYNHKKIRKQFASKHTFSTGSDCEVIIPLYEEY-------GEDFVDMLDGVF 129

Query: 135 VFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGKSFAPFPKGCFF 194
            FVLYD+ +KT   A DA G  PL+ G  S+G + +S +++ + + C   F  FP G  +
Sbjct: 130 AFVLYDTRTKTYMAARDAIGVNPLYIGRGSDGAVWISSEMKALNEDC-VEFEIFPPGHLY 189

Query: 195 TT-SGGLRSFEHP 204
           ++ +GGLR +  P
Sbjct: 190 SSAAGGLRRWYKP 194

BLAST of MC05g0497 vs. ExPASy Swiss-Prot
Match: Q43011 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0265000 PE=2 SV=3)

HSP 1 Score: 51.2 bits (121), Expect = 1.9e-05
Identity = 35/126 (27.78%), Postives = 60/126 (47.62%), Query Frame = 0

Query: 81  GHIENVAQLKQQYGLNK--AANEVIIVIEAYRTLRDRGPYPADQVVRDIQGKFVFVLYDS 140
           G I N  +LK     +K   A++  ++   Y           ++ V  + G F FVL D+
Sbjct: 76  GEIYNHEELKANLKSHKFQTASDCEVIAHLYEEY-------GEEFVDMLDGMFAFVLLDT 135

Query: 141 SSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGKSFAPFPKGCFFTT-SGGL 200
             K+   A DA G  PL+ G   +G +  S +++ +   C + F  FP G  +++ +GGL
Sbjct: 136 RDKSFIAARDAIGICPLYMGWGLDGSVWFSSEMKALSDDC-ERFISFPPGHLYSSKTGGL 193

Query: 201 RSFEHP 204
           R + +P
Sbjct: 196 RRWYNP 193

BLAST of MC05g0497 vs. ExPASy Swiss-Prot
Match: Q54MB4 (Probable asparagine synthetase [glutamine-hydrolyzing] OS=Dictyostelium discoideum OX=44689 GN=asns PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.3e-05
Identity = 25/73 (34.25%), Postives = 40/73 (54.79%), Query Frame = 0

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           D  V  + G F FV+Y+  + +   A D  G VPL+ G   +G +  S +++ +K  C K
Sbjct: 114 DDFVHMLDGDFAFVVYNKKANSFLAARDPIGVVPLYIGWHKDGSIWFSSEMKAIKDDCYK 173

Query: 181 SFAPFPKGCFFTT 194
            F PFP G +F++
Sbjct: 174 -FQPFPPGHYFSS 185

BLAST of MC05g0497 vs. NCBI nr
Match: XP_022138225.1 (stem-specific protein TSJT1 [Momordica charantia])

HSP 1 Score: 514 bits (1323), Expect = 6.34e-184
Identity = 250/250 (100.00%), Postives = 250/250 (100.00%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK
Sbjct: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240

Query: 241 GSAANWSSNY 250
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of MC05g0497 vs. NCBI nr
Match: XP_022947693.1 (stem-specific protein TSJT1-like [Cucurbita moschata] >XP_022974438.1 stem-specific protein TSJT1-like [Cucurbita maxima] >XP_023540849.1 stem-specific protein TSJT1-like [Cucurbita pepo subsp. pepo] >KAG6597414.1 Stem-specific protein TSJT1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 487 bits (1253), Expect = 2.99e-173
Identity = 235/250 (94.00%), Postives = 243/250 (97.20%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLAVFDKSVAK+P+ALQSPHSDSVWALKDGIL +HF S+YP SVIVNLGSAGLLAYSVEK
Sbjct: 1   MLAVFDKSVAKSPDALQSPHSDSVWALKDGILAKHFSSVYPDSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           +NPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  KNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           DQVVRDIQGKFVFVL+DSSSK AFFASDADGSVPLHWG+DSEG LVLSDD+E MKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLFDSSSKAAFFASDADGSVPLHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFA FPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV
Sbjct: 181 SFASFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240

Query: 241 GSAANWSSNY 250
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of MC05g0497 vs. NCBI nr
Match: XP_038878034.1 (stem-specific protein TSJT1 [Benincasa hispida])

HSP 1 Score: 487 bits (1253), Expect = 2.99e-173
Identity = 235/250 (94.00%), Postives = 243/250 (97.20%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLAVFDKSVAK+P+ALQSP SDSVWALKDGIL +HF S+YP SVIVNLGSAGLLAYSVEK
Sbjct: 1   MLAVFDKSVAKSPDALQSPESDSVWALKDGILAQHFSSVYPGSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVP HWG+DSEG LVLSDD+ETMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPFHWGTDSEGQLVLSDDVETMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRS+EHPLNELKPVPRVDSSG+VCGANFKVD EARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240

Query: 241 GSAANWSSNY 250
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of MC05g0497 vs. NCBI nr
Match: KAG7028873.1 (Stem-specific protein TSJT1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 487 bits (1253), Expect = 2.06e-172
Identity = 235/250 (94.00%), Postives = 243/250 (97.20%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLAVFDKSVAK+P+ALQSPHSDSVWALKDGIL +HF S+YP SVIVNLGSAGLLAYSVEK
Sbjct: 54  MLAVFDKSVAKSPDALQSPHSDSVWALKDGILAKHFSSVYPDSVIVNLGSAGLLAYSVEK 113

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           +NPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 114 KNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 173

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           DQVVRDIQGKFVFVL+DSSSK AFFASDADGSVPLHWG+DSEG LVLSDD+E MKKGCGK
Sbjct: 174 DQVVRDIQGKFVFVLFDSSSKAAFFASDADGSVPLHWGTDSEGQLVLSDDVEIMKKGCGK 233

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFA FPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV
Sbjct: 234 SFASFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 293

Query: 241 GSAANWSSNY 250
           GSAANWSSNY
Sbjct: 294 GSAANWSSNY 303

BLAST of MC05g0497 vs. NCBI nr
Match: XP_008438612.1 (PREDICTED: stem-specific protein TSJT1 [Cucumis melo] >KAA0049327.1 stem-specific protein TSJT1 [Cucumis melo var. makuwa] >TYK17231.1 stem-specific protein TSJT1 [Cucumis melo var. makuwa])

HSP 1 Score: 479 bits (1234), Expect = 2.35e-170
Identity = 231/250 (92.40%), Postives = 240/250 (96.00%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLAVF+KSVAK+P+ALQSP SDS WALKDGIL +HF S YP SVIVNLGSAGLLAYSVEK
Sbjct: 1   MLAVFEKSVAKSPDALQSPESDSTWALKDGILAQHFSSAYPGSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           DQVVRDIQGKFVFVLYDSSSKT+FFASDADGSVP HWG+DSEG LVLSDD+E MKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRS+EHPLNELKPVPRVDSSG+VCGANFKVD EARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240

Query: 241 GSAANWSSNY 250
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of MC05g0497 vs. ExPASy TrEMBL
Match: A0A6J1C8V4 (stem-specific protein TSJT1 OS=Momordica charantia OX=3673 GN=LOC111009449 PE=4 SV=1)

HSP 1 Score: 514 bits (1323), Expect = 3.07e-184
Identity = 250/250 (100.00%), Postives = 250/250 (100.00%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK
Sbjct: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240

Query: 241 GSAANWSSNY 250
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of MC05g0497 vs. ExPASy TrEMBL
Match: A0A6J1IG81 (stem-specific protein TSJT1-like OS=Cucurbita maxima OX=3661 GN=LOC111473103 PE=4 SV=1)

HSP 1 Score: 487 bits (1253), Expect = 1.45e-173
Identity = 235/250 (94.00%), Postives = 243/250 (97.20%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLAVFDKSVAK+P+ALQSPHSDSVWALKDGIL +HF S+YP SVIVNLGSAGLLAYSVEK
Sbjct: 1   MLAVFDKSVAKSPDALQSPHSDSVWALKDGILAKHFSSVYPDSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           +NPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  KNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           DQVVRDIQGKFVFVL+DSSSK AFFASDADGSVPLHWG+DSEG LVLSDD+E MKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLFDSSSKAAFFASDADGSVPLHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFA FPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV
Sbjct: 181 SFASFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240

Query: 241 GSAANWSSNY 250
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of MC05g0497 vs. ExPASy TrEMBL
Match: A0A6J1G7B2 (stem-specific protein TSJT1-like OS=Cucurbita moschata OX=3662 GN=LOC111451479 PE=4 SV=1)

HSP 1 Score: 487 bits (1253), Expect = 1.45e-173
Identity = 235/250 (94.00%), Postives = 243/250 (97.20%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLAVFDKSVAK+P+ALQSPHSDSVWALKDGIL +HF S+YP SVIVNLGSAGLLAYSVEK
Sbjct: 1   MLAVFDKSVAKSPDALQSPHSDSVWALKDGILAKHFSSVYPDSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           +NPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  KNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           DQVVRDIQGKFVFVL+DSSSK AFFASDADGSVPLHWG+DSEG LVLSDD+E MKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLFDSSSKAAFFASDADGSVPLHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFA FPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV
Sbjct: 181 SFASFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240

Query: 241 GSAANWSSNY 250
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of MC05g0497 vs. ExPASy TrEMBL
Match: A0A5D3CZ34 (Stem-specific protein TSJT1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00820 PE=4 SV=1)

HSP 1 Score: 479 bits (1234), Expect = 1.14e-170
Identity = 231/250 (92.40%), Postives = 240/250 (96.00%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLAVF+KSVAK+P+ALQSP SDS WALKDGIL +HF S YP SVIVNLGSAGLLAYSVEK
Sbjct: 1   MLAVFEKSVAKSPDALQSPESDSTWALKDGILAQHFSSAYPGSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           DQVVRDIQGKFVFVLYDSSSKT+FFASDADGSVP HWG+DSEG LVLSDD+E MKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRS+EHPLNELKPVPRVDSSG+VCGANFKVD EARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240

Query: 241 GSAANWSSNY 250
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of MC05g0497 vs. ExPASy TrEMBL
Match: A0A1S3AXH1 (stem-specific protein TSJT1 OS=Cucumis melo OX=3656 GN=LOC103483669 PE=4 SV=1)

HSP 1 Score: 479 bits (1234), Expect = 1.14e-170
Identity = 231/250 (92.40%), Postives = 240/250 (96.00%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLAVF+KSVAK+P+ALQSP SDS WALKDGIL +HF S YP SVIVNLGSAGLLAYSVEK
Sbjct: 1   MLAVFEKSVAKSPDALQSPESDSTWALKDGILAQHFSSAYPGSVIVNLGSAGLLAYSVEK 60

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA
Sbjct: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           DQVVRDIQGKFVFVLYDSSSKT+FFASDADGSVP HWG+DSEG LVLSDD+E MKKGCGK
Sbjct: 121 DQVVRDIQGKFVFVLYDSSSKTSFFASDADGSVPFHWGTDSEGQLVLSDDVEIMKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFAPFPKGCFFTTSGGLRS+EHPLNELKPVPRVDSSG+VCGANFKVD EARKESSGMPRV
Sbjct: 181 SFAPFPKGCFFTTSGGLRSYEHPLNELKPVPRVDSSGNVCGANFKVDAEARKESSGMPRV 240

Query: 241 GSAANWSSNY 250
           GSAANWSSNY
Sbjct: 241 GSAANWSSNY 250

BLAST of MC05g0497 vs. TAIR 10
Match: AT3G22850.1 (Aluminium induced protein with YGL and LRDR motifs )

HSP 1 Score: 389.0 bits (998), Expect = 2.8e-108
Identity = 184/247 (74.49%), Postives = 208/247 (84.21%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVEK 60
           MLA+FDK+VAKTPEALQ     SV ALKD  L  HF S+YP +V +NLGS+G +A S+EK
Sbjct: 1   MLAIFDKNVAKTPEALQGQEGGSVCALKDRFLPNHFSSVYPGAVTINLGSSGFIACSLEK 60

Query: 61  QNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYPA 120
           QNPLLPRLFAVVDD+FC+FQGHIENV  LKQQYGL K A EV IVIEAYRTLRDRGPY A
Sbjct: 61  QNPLLPRLFAVVDDMFCIFQGHIENVPILKQQYGLTKTATEVTIVIEAYRTLRDRGPYSA 120

Query: 121 DQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCGK 180
           +QVVRD QGKF F+LYD S++  F A D DGSVPL+WG+D+EGHLV+SDD+ET+KKGCGK
Sbjct: 121 EQVVRDFQGKFGFMLYDCSTQNVFLAGDVDGSVPLYWGTDAEGHLVVSDDVETVKKGCGK 180

Query: 181 SFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPRV 240
           SFAPFPKGCFFT+SGGLRS+EHP NELKPVPRVDSSG VCG  FKVD EA+KE+  MPRV
Sbjct: 181 SFAPFPKGCFFTSSGGLRSYEHPSNELKPVPRVDSSGEVCGVTFKVDSEAKKEA--MPRV 240

Query: 241 GSAANWS 248
           GS  NWS
Sbjct: 241 GSVQNWS 245

BLAST of MC05g0497 vs. TAIR 10
Match: AT5G43830.1 (Aluminium induced protein with YGL and LRDR motifs )

HSP 1 Score: 372.9 bits (956), Expect = 2.1e-103
Identity = 175/250 (70.00%), Postives = 205/250 (82.00%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPH-SDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYSVE 60
           MLAVF+K+VA +PEALQSPH S+S +ALKDG L  HF S+ P SV +N GS+G +AYS++
Sbjct: 1   MLAVFEKTVANSPEALQSPHSSESAFALKDGSLATHFASVNPNSVTLNFGSSGFVAYSLD 60

Query: 61  KQNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYP 120
             +P +PRLFAVVDDIFCLFQGHIEN+  LKQQYGLNK  NE IIVIEAYRTLRDRGPYP
Sbjct: 61  NPDPRVPRLFAVVDDIFCLFQGHIENLPFLKQQYGLNKITNEAIIVIEAYRTLRDRGPYP 120

Query: 121 ADQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCG 180
            D+VVRD  GKF F+L+DS  KT F A+DADGSVP  WG+D+EGHLV SD+ E +KKGC 
Sbjct: 121 VDKVVRDFHGKFAFILFDSVKKTVFAAADADGSVPFFWGTDAEGHLVFSDNTEMVKKGCA 180

Query: 181 KSFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSGMPR 240
           KS+ PFPKGCFFT+SGGLRSFEHP NELKPVPRVDSSG VCGA FKVD E ++E + MPR
Sbjct: 181 KSYGPFPKGCFFTSSGGLRSFEHPKNELKPVPRVDSSGDVCGATFKVDAETKREGTKMPR 240

Query: 241 VGSAANWSSN 250
           V S+ NW+ +
Sbjct: 241 VDSSQNWAGH 250

BLAST of MC05g0497 vs. TAIR 10
Match: AT4G27450.1 (Aluminium induced protein with YGL and LRDR motifs )

HSP 1 Score: 250.4 bits (638), Expect = 1.6e-66
Identity = 129/252 (51.19%), Postives = 167/252 (66.27%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVW---ALKDGILGRHFGSLYPASVI-VNLGSAGLLAY 60
           MLA+F ++ A  PE L SP S+       L +  L   F   YP +   ++ G A +LAY
Sbjct: 1   MLAIFHEAFAHPPEELNSPASEKCSKQPKLPEETL-NDFLLRYPLNTFSMSFGQAAVLAY 60

Query: 61  SVEKQN-PLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDR 120
                +  +  RLF   DDI+CLF G + N+ QL +QYGL K  NE + VIEAYRTLRDR
Sbjct: 61  VRPSASFSIHQRLFCGFDDIYCLFFGSLNNLCQLNKQYGLTKTTNEAMFVIEAYRTLRDR 120

Query: 121 GPYPADQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMK 180
           GPYPADQVV+D+ G F FV+YDS + + F A  +DG V L+WG  ++G +V+SDD++ +K
Sbjct: 121 GPYPADQVVKDLDGSFSFVVYDSKAGSVFTALGSDGGVKLYWGIAADGSVVISDDLDVIK 180

Query: 181 KGCGKSFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESS 240
           +GC KSFAPFP GC F + GGL SFEHP+N++K +PRVDS G +CGANFKVD   R  S 
Sbjct: 181 EGCAKSFAPFPTGCMFHSEGGLMSFEHPMNKIKAMPRVDSEGVLCGANFKVDVYNRVNS- 240

Query: 241 GMPRVGSAANWS 248
            +PR GS ANWS
Sbjct: 241 -IPRRGSEANWS 249

BLAST of MC05g0497 vs. TAIR 10
Match: AT3G15450.1 (Aluminium induced protein with YGL and LRDR motifs )

HSP 1 Score: 235.3 bits (599), Expect = 5.2e-62
Identity = 117/251 (46.61%), Postives = 162/251 (64.54%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSP---HSDSVWALKDGILGRHFGSLYPASVIVNLGSAGLLAYS 60
           MLA+F K+ A  PE L SP    S     L    L          +  +N G + +LAY+
Sbjct: 1   MLAIFQKAFAHPPEELNSPASHFSGKTPKLPGETLSDFLSHHQNNAFSMNFGDSAVLAYA 60

Query: 61  VEKQNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLN-KAANEVIIVIEAYRTLRDRG 120
             ++  L  RLF  +D I+C+F G + N+  L +QYGL+ K +NE + VIEAYRTLRDRG
Sbjct: 61  -RQETSLRQRLFCGLDGIYCMFLGRLNNLCTLNRQYGLSGKNSNEAMFVIEAYRTLRDRG 120

Query: 121 PYPADQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKK 180
           PYPADQV+R ++G F FV+YD+ + + F A  +DG   L+WG   +G +V+SDDI+ +K+
Sbjct: 121 PYPADQVLRGLEGSFAFVVYDTQTSSVFSALSSDGGESLYWGISGDGSVVMSDDIQIIKQ 180

Query: 181 GCGKSFAPFPKGCFFTTSGGLRSFEHPLNELKPVPRVDSSGHVCGANFKVDEEARKESSG 240
           GC KSFAPFP GC F +  GL+SF+HP N +K +PR+DS G +CGA+FKVD  A  + + 
Sbjct: 181 GCAKSFAPFPNGCMFHSETGLKSFDHPTNMMKAMPRIDSEGVLCGASFKVD--ACSKINS 240

Query: 241 MPRVGSAANWS 248
           +PR GS ANW+
Sbjct: 241 IPRRGSEANWA 248

BLAST of MC05g0497 vs. TAIR 10
Match: AT5G19140.1 (Aluminium induced protein with YGL and LRDR motifs )

HSP 1 Score: 219.9 bits (559), Expect = 2.2e-57
Identity = 108/229 (47.16%), Postives = 150/229 (65.50%), Query Frame = 0

Query: 1   MLAVFDKSVAKTPEALQSPHSDSVWALKDG-ILGRHFGSLYPASVIVNLGSAGLLAYSVE 60
           ML +F  ++   PE L +  S +      G  L   F    P++V V +G    LAYS  
Sbjct: 1   MLGIFSGAIVSPPEELVAAGSRTPSPKTTGSTLVNRFVEKNPSAVSVQVGDYVQLAYSHH 60

Query: 61  KQNPLLPRLFAVVDDIFCLFQGHIENVAQLKQQYGLNKAANEVIIVIEAYRTLRDRGPYP 120
            ++PL PR F   D+IFCLFQG ++N+  LKQQYGL K ANEV++VIEAY+TLRDR PYP
Sbjct: 61  NESPLRPRSFGAKDEIFCLFQGSLDNLGSLKQQYGLAKNANEVLLVIEAYKTLRDRAPYP 120

Query: 121 ADQVVRDIQGKFVFVLYDSSSKTAFFASDADGSVPLHWGSDSEGHLVLSDDIETMKKGCG 180
           A+ VV  + G F FV++D S+ T F ASD  G VPL+WG  ++G++  +DD++ +K  CG
Sbjct: 121 ANHVVAHLSGDFAFVVFDKSTSTLFVASDQVGKVPLYWGITADGYVAFADDVDLLKGACG 180

Query: 181 KSFAPFPKGCFFTTS-GGLRSFEHPLNELKPVPRVDSSGHVCGANFKVD 228
           KS A FP+GC+++T+ GGLRSFE+P N++  VP   + G + GA FKV+
Sbjct: 181 KSLASFPQGCYYSTALGGLRSFENPKNKITAVPA--NEGEIWGATFKVE 227

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P248052.8e-2839.19Stem-specific protein TSJT1 OS=Nicotiana tabacum OX=4097 GN=TSJT1 PE=2 SV=1[more]
P221061.2e-0726.98Asparagine synthetase B [glutamine-hydrolyzing] OS=Escherichia coli (strain K12)... [more]
Q10MX37.9e-0727.82Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Oryza sativa subsp. japonica ... [more]
Q430111.9e-0527.78Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica ... [more]
Q54MB43.3e-0534.25Probable asparagine synthetase [glutamine-hydrolyzing] OS=Dictyostelium discoide... [more]
Match NameE-valueIdentityDescription
XP_022138225.16.34e-184100.00stem-specific protein TSJT1 [Momordica charantia][more]
XP_022947693.12.99e-17394.00stem-specific protein TSJT1-like [Cucurbita moschata] >XP_022974438.1 stem-speci... [more]
XP_038878034.12.99e-17394.00stem-specific protein TSJT1 [Benincasa hispida][more]
KAG7028873.12.06e-17294.00Stem-specific protein TSJT1, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_008438612.12.35e-17092.40PREDICTED: stem-specific protein TSJT1 [Cucumis melo] >KAA0049327.1 stem-specifi... [more]
Match NameE-valueIdentityDescription
A0A6J1C8V43.07e-184100.00stem-specific protein TSJT1 OS=Momordica charantia OX=3673 GN=LOC111009449 PE=4 ... [more]
A0A6J1IG811.45e-17394.00stem-specific protein TSJT1-like OS=Cucurbita maxima OX=3661 GN=LOC111473103 PE=... [more]
A0A6J1G7B21.45e-17394.00stem-specific protein TSJT1-like OS=Cucurbita moschata OX=3662 GN=LOC111451479 P... [more]
A0A5D3CZ341.14e-17092.40Stem-specific protein TSJT1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AXH11.14e-17092.40stem-specific protein TSJT1 OS=Cucumis melo OX=3656 GN=LOC103483669 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G22850.12.8e-10874.49Aluminium induced protein with YGL and LRDR motifs [more]
AT5G43830.12.1e-10370.00Aluminium induced protein with YGL and LRDR motifs [more]
AT4G27450.11.6e-6651.19Aluminium induced protein with YGL and LRDR motifs [more]
AT3G15450.15.2e-6246.61Aluminium induced protein with YGL and LRDR motifs [more]
AT5G19140.12.2e-5747.16Aluminium induced protein with YGL and LRDR motifs [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024286Domain of unknown function DUF3700SMARTSM01172DUF3700_2coord: 2..226
e-value: 3.9E-150
score: 514.7
IPR024286Domain of unknown function DUF3700PFAMPF12481DUF3700coord: 2..226
e-value: 6.4E-111
score: 369.3
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 56..201
e-value: 1.7E-14
score: 55.5
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 70..198
NoneNo IPR availablePIRSRPIRSR001589-2PIRSR001589-2coord: 77..191
e-value: 6.1E-7
score: 26.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 231..250
NoneNo IPR availablePANTHERPTHR45952:SF4ALUMINUM INDUCED PROTEIN WITH YGL AND LRDR MOTIFScoord: 1..250
NoneNo IPR availableCDDcd01910Wali7coord: 2..226
e-value: 8.52919E-141
score: 392.058
IPR044828Stem-specific protein TSJT1-likePANTHERPTHR45952ALUMINUM INDUCED PROTEIN WITH YGL AND LRDR MOTIFScoord: 1..250

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0497.1MC05g0497.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity