MC05g0475 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0475
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	P-loop nucleoside triphosphate hydrolase superfamily protein
Location	MC05: 3490688 .. 3494212 (+)
RNA-Seq Expression	MC05g0475
Synteny	MC05g0475

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGCTTTCCAAATTCTCTGTTGATTTCTCAGACTCTTTCAAATCACATAGAAAGTGCGAGTCTGACCCACGTACTTTTCAAATCCGTGGAGCTCAATATATTTATGGAGCTCCCATTTCCAACCACCATCACAGCCGATGAACAAAGAATCAAAGATCCTCATCTTCTTCCTCCGATCTCTCTGCTTTTTTGATTTTTTTATCATGAAGCACCAAGGTAAAGCTAATTTTTACAAACAATCCTTTGATTTGATCTTTGAAGTTCACCAAATGTTGAGGAGTAGTAACTATAACTCTCTTGCTCTGTGATTGATTAGATGGCGGTTACGGGAAAGTCATTCCATCGTCGCACTTTCTTACCCATTCTTTTGCCGCCGCCGGAAAACGCCACTACCGGCGGACTTCGAATGGTGTAACCAGCTATCAGAAGTTGGTTCCGCATCTACACAGAACGGAGTCCGGCCGCGTTGATTTCTTGGAGAGATTTTCCCATTATGTTGGTAAAATCATCCGCCATTACTATCTATAAATTAAAGTACTCTCTATGTGTAGAATGGAAAATCTAACCCTTATATTTGGGGTTTTCTTTTTTTTGTTTGGACTTTTTGCAGCGAGGCAATTGGGGATTTCTGACGCGGATGAGTGTCCTCAACTATGTAAGTTAGCAAACAACTATTTGAGGAAGACGAAGGGGTGCGAGGAAGAAATTTATGCCTATTTTGCGAGTGAAGTCGAGGCAGAATCTCTGTATGTGAAGTTAATGGAGGAGTTTGATCAATGCATCTTGAGTTATTTCGCATTCCATTGGAGCCAAGCTTCCTTGATGATTACTCAGGTAAAATTCATAAACCCCAAAGGAAAGAGGAAAAAAAAAAAGTCAACATGATTTTAGAATCCCATCATATCCCTTGATTAACGGTGAATTTAATGATAGGTTTTGGGTGTTGACTCCGAGCACAAAAGGCTCAAAGACCTCGTGGTGGCAGCGACTAGGTGATTTCCATTTTCCATCTTTTGTCAATTTGCCTGAGAAGTCATAATTGGTTTCTGAGTTTGAACTTTGAATGCACCAGCACCCCTTTTTGTTGAACCATGATGCATCTTCTAGAATTGAGATACACATCCACTGTATGGCTGTAATATAAATGAAGATTCTGAGTTTCTTGTTTCCCTTTTTGGTTTCTGGCTAACTGATAGGAAGCAGAGATTCGATAGGGTGAGTAAGGATCTGAAAGTGACTCGGGTCTTCTCTACTTTGGTGGAGGAGATGAAGAAGATTGGCTGTGCTCCGTCCAAGGGCGAGTCGAGATGCTCCCACACGATACCGCCAGTTCAAAGAGAGAGGAGCCCTGTGCTTCTTCTTATGGGTGGTGGCATGGGGGCTGGCAAGAGCACTGTCCTCAAAGACATCTCGAAAGAGTGCGTAAATCAATCTCAAATCTCGTTCATTTTGCAAAAACGAAACCTCTTTTAGATCAAAGTTTGTTGATAAACAGTCACTGATTATGCAGGCCTTTTTGGTTGGAAGCAGCAACAAACCCAGTATTTGTAGAGGCAGATGCCTTCAAAGAGACTGATGTTATCTACAAAGCCATCAGCTCGATGGGTTACCACGACGACATGCTTCAGACTGCCGAATTGGTGAGTTTCTCTCCTCTCCAGCACAACCACACACTTATTTTCCCCAATTTACATGCCCAATCTTTTGAACACGAATAAGCTAACTAACTACACGATGAACCGCAACAGGTACACCAGTCATCAATCGACGCTGCATCTTCCTTGCTGGTCACAGCGTTAAACGAGGGACGGGATGTGATCTTGGACAGCACCCTGTCCTGGGATCCCTACGTCATGCAAACAATCGAAATGGCTCGAAACGTTCACAAGCGTCGATATCGAATGGGAGTTGGGTACAAGGTAGAGAATGGAAATATAACAGAGAATTACTGGGAACCTGTAAGTGAAGAAGAGGAGGAGGAATTGCAGGAGGATGGAGAAATGAACAATAGAATGCCATATAGAATAGAGTTGGTTGGAGTTGTTTGTGATGCTCATCTAGCTGTTGTTAGAGGAATCAGGTAAAGATACAAGTCAACTTTTGAAGCTTGAGCTTACAGTAATGACATTTTAAGGCCAGTTTTGGTTCTAATAAAAGCTGTAATGGATTTGAACAGGAGAGCCATAATGATGGGAAGGGCTGTGAGGGTGAATTCCCAGTTGCAATCCCACAAGAGATTTGCCAATGCTTTTCCAAAATACTCTGAAGTTGTTGATAGTGTCAGGCTCTACTCCACAAATTCCATTGGCAACCCACCTAAGGTATGTCTTGATTTATAAAATTATTCCTATGCTTTTACTAACTATATCCACACGACAAACCTATAGAATCAATTTTAATTGAGTTGCCGTGATGTGACCCCGAGTAGCTATGCTCGTCTCCTATGAGACTAGATAGATTTTACTCGTTGGTGTCAATTAGGTGGTCTGTGGACTTTTGTTGGGAATGGCCAAGATTCATCTCAGATACAGTACAAATAGAATCCGATTCTCTTTTCATATTAGATTTCTTCATTTCCCATACAGATCAAGAAATTGAATTTCAATTATTTGTGGAAACATATAAATTGGTAGAACCATTGATAAAAGAAAGAGATGCTGTATATGAATCACTCACATATTCTTCTGAATTATACGTATCTGCAGAAGATCTCCTTTGACCAACATCGATTCAACTATATATTTTTAGTACAACGACTATGTTTTTTTTAGTACAAGATTACCTGTAGTCTTGGTTAATGACATGGATTAAATAAAAATGTATTACCGAATTAATTTTAGAGATGTAATGAATGTAGTTCTAGAAGTATGGACTAATATTTGGAGAATACTAATTCCCACTTTGAATGTATTTGCAAGAAAATGTGAAATTGCAGTTATATTAAACTATTAAATATTTCAATATTTAAACGTGTTTCCAATTTCGCAGTTGCTCATTAGAAAAGACGGCGACGATGACCCGTCTCAAATGGTTGACCCGGAAGCACATGCGTACTTGTCAACGCTGAGCAATTTGAACGCCGCCGCGGAGTCTGTTTACGAGCTTTACCCGAACCCGAGTCCGTTTGTGGAACCCGAGTCCGTTTGGAAAGAAATTGGTGTGTCCCCTTCGAGGATCCATTCTCAGAAAGAGCTCAGATCTGCGATTCAGAAGCTAGAAAGTTCAAGATCAATCTTAGATGAAACTGTACAGTGACAAGAACTTGTAAATTTCATGGCGATTGAGGTGAAGTGTAAAATTAATGATGATAAATCTTCACTTTCTTGAAAGAAGGAGCGTTTGCAGTTTTGAAAAATCTGACGAACGATACAATTTTTTTTTTCTTTTTTTGCCTCTCCTTTTTCCACATTTGCGTATTGATTCATTAGAAGTGTAGATTCGTTCGTAAGAATGTTCGAACCATGTGAGAGAGGCATTGCTGGCCATTCCAAATGAATATATGATATCAAACCTTAAGTTCTGGAT

mRNA sequence

TGGCTTTCCAAATTCTCTGTTGATTTCTCAGACTCTTTCAAATCACATAGAAAGTGCGAGTCTGACCCACGTACTTTTCAAATCCGTGGAGCTCAATATATTTATGGAGCTCCCATTTCCAACCACCATCACAGCCGATGAACAAAGAATCAAAGATCCTCATCTTCTTCCTCCGATCTCTCTGCTTTTTTGATTTTTTTATCATGAAGCACCAAGATGGCGGTTACGGGAAAGTCATTCCATCGTCGCACTTTCTTACCCATTCTTTTGCCGCCGCCGGAAAACGCCACTACCGGCGGACTTCGAATGGTGTAACCAGCTATCAGAAGTTGGTTCCGCATCTACACAGAACGGAGTCCGGCCGCGTTGATTTCTTGGAGAGATTTTCCCATTATGTTGCGAGGCAATTGGGGATTTCTGACGCGGATGAGTGTCCTCAACTATGTAAGTTAGCAAACAACTATTTGAGGAAGACGAAGGGGTGCGAGGAAGAAATTTATGCCTATTTTGCGAGTGAAGTCGAGGCAGAATCTCTGTATGTGAAGTTAATGGAGGAGTTTGATCAATGCATCTTGAGTTATTTCGCATTCCATTGGAGCCAAGCTTCCTTGATGATTACTCAGGTTTTGGGTGTTGACTCCGAGCACAAAAGGCTCAAAGACCTCGTGGTGGCAGCGACTAGGAAGCAGAGATTCGATAGGGTGAGTAAGGATCTGAAAGTGACTCGGGTCTTCTCTACTTTGGTGGAGGAGATGAAGAAGATTGGCTGTGCTCCGTCCAAGGGCGAGTCGAGATGCTCCCACACGATACCGCCAGTTCAAAGAGAGAGGAGCCCTGTGCTTCTTCTTATGGGTGGTGGCATGGGGGCTGGCAAGAGCACTGTCCTCAAAGACATCTCGAAAGAGCCTTTTTGGTTGGAAGCAGCAACAAACCCAGTATTTGTAGAGGCAGATGCCTTCAAAGAGACTGATGTTATCTACAAAGCCATCAGCTCGATGGGTTACCACGACGACATGCTTCAGACTGCCGAATTGGTACACCAGTCATCAATCGACGCTGCATCTTCCTTGCTGGTCACAGCGTTAAACGAGGGACGGGATGTGATCTTGGACAGCACCCTGTCCTGGGATCCCTACGTCATGCAAACAATCGAAATGGCTCGAAACGTTCACAAGCGTCGATATCGAATGGGAGTTGGGTACAAGGTAGAGAATGGAAATATAACAGAGAATTACTGGGAACCTGTAAGTGAAGAAGAGGAGGAGGAATTGCAGGAGGATGGAGAAATGAACAATAGAATGCCATATAGAATAGAGTTGGTTGGAGTTGTTTGTGATGCTCATCTAGCTGTTGTTAGAGGAATCAGGAGAGCCATAATGATGGGAAGGGCTGTGAGGGTGAATTCCCAGTTGCAATCCCACAAGAGATTTGCCAATGCTTTTCCAAAATACTCTGAAGTTGTTGATAGTGTCAGGCTCTACTCCACAAATTCCATTGGCAACCCACCTAAGTTGCTCATTAGAAAAGACGGCGACGATGACCCGTCTCAAATGGTTGACCCGGAAGCACATGCGTACTTGTCAACGCTGAGCAATTTGAACGCCGCCGCGGAGTCTGTTTACGAGCTTTACCCGAACCCGAGTCCGTTTGTGGAACCCGAGTCCGTTTGGAAAGAAATTGGTGTGTCCCCTTCGAGGATCCATTCTCAGAAAGAGCTCAGATCTGCGATTCAGAAGCTAGAAAGTTCAAGATCAATCTTAGATGAAACTGTACAGTGACAAGAACTTGTAAATTTCATGGCGATTGAGGTGAAGTGTAAAATTAATGATGATAAATCTTCACTTTCTTGAAAGAAGGAGCGTTTGCAGTTTTGAAAAATCTGACGAACGATACAATTTTTTTTTTCTTTTTTTGCCTCTCCTTTTTCCACATTTGCGTATTGATTCATTAGAAGTGTAGATTCGTTCGTAAGAATGTTCGAACCATGTGAGAGAGGCATTGCTGGCCATTCCAAATGAATATATGATATCAAACCTTAAGTTCTGGAT

Coding sequence (CDS)

ATGAACAAAGAATCAAAGATCCTCATCTTCTTCCTCCGATCTCTCTGCTTTTTTGATTTTTTTATCATGAAGCACCAAGATGGCGGTTACGGGAAAGTCATTCCATCGTCGCACTTTCTTACCCATTCTTTTGCCGCCGCCGGAAAACGCCACTACCGGCGGACTTCGAATGGTGTAACCAGCTATCAGAAGTTGGTTCCGCATCTACACAGAACGGAGTCCGGCCGCGTTGATTTCTTGGAGAGATTTTCCCATTATGTTGCGAGGCAATTGGGGATTTCTGACGCGGATGAGTGTCCTCAACTATGTAAGTTAGCAAACAACTATTTGAGGAAGACGAAGGGGTGCGAGGAAGAAATTTATGCCTATTTTGCGAGTGAAGTCGAGGCAGAATCTCTGTATGTGAAGTTAATGGAGGAGTTTGATCAATGCATCTTGAGTTATTTCGCATTCCATTGGAGCCAAGCTTCCTTGATGATTACTCAGGTTTTGGGTGTTGACTCCGAGCACAAAAGGCTCAAAGACCTCGTGGTGGCAGCGACTAGGAAGCAGAGATTCGATAGGGTGAGTAAGGATCTGAAAGTGACTCGGGTCTTCTCTACTTTGGTGGAGGAGATGAAGAAGATTGGCTGTGCTCCGTCCAAGGGCGAGTCGAGATGCTCCCACACGATACCGCCAGTTCAAAGAGAGAGGAGCCCTGTGCTTCTTCTTATGGGTGGTGGCATGGGGGCTGGCAAGAGCACTGTCCTCAAAGACATCTCGAAAGAGCCTTTTTGGTTGGAAGCAGCAACAAACCCAGTATTTGTAGAGGCAGATGCCTTCAAAGAGACTGATGTTATCTACAAAGCCATCAGCTCGATGGGTTACCACGACGACATGCTTCAGACTGCCGAATTGGTACACCAGTCATCAATCGACGCTGCATCTTCCTTGCTGGTCACAGCGTTAAACGAGGGACGGGATGTGATCTTGGACAGCACCCTGTCCTGGGATCCCTACGTCATGCAAACAATCGAAATGGCTCGAAACGTTCACAAGCGTCGATATCGAATGGGAGTTGGGTACAAGGTAGAGAATGGAAATATAACAGAGAATTACTGGGAACCTGTAAGTGAAGAAGAGGAGGAGGAATTGCAGGAGGATGGAGAAATGAACAATAGAATGCCATATAGAATAGAGTTGGTTGGAGTTGTTTGTGATGCTCATCTAGCTGTTGTTAGAGGAATCAGGAGAGCCATAATGATGGGAAGGGCTGTGAGGGTGAATTCCCAGTTGCAATCCCACAAGAGATTTGCCAATGCTTTTCCAAAATACTCTGAAGTTGTTGATAGTGTCAGGCTCTACTCCACAAATTCCATTGGCAACCCACCTAAGTTGCTCATTAGAAAAGACGGCGACGATGACCCGTCTCAAATGGTTGACCCGGAAGCACATGCGTACTTGTCAACGCTGAGCAATTTGAACGCCGCCGCGGAGTCTGTTTACGAGCTTTACCCGAACCCGAGTCCGTTTGTGGAACCCGAGTCCGTTTGGAAAGAAATTGGTGTGTCCCCTTCGAGGATCCATTCTCAGAAAGAGCTCAGATCTGCGATTCAGAAGCTAGAAAGTTCAAGATCAATCTTAGATGAAACTGTACAGTGA

Protein sequence

MNKESKILIFFLRSLCFFDFFIMKHQDGGYGKVIPSSHFLTHSFAAAGKRHYRRTSNGVTSYQKLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAATRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPVQRERSPVLLLMGGGMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENGNITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAYLSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSILDETVQ

Homology

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BLAST of MC05g0475 vs. ExPASy Swiss-Prot
Match: Q0WUY1 (Calmodulin calcium-dependent NAD kinase OS=Arabidopsis thaliana OX=3702 GN=NADKC PE=1 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 3.8e-163
Identity = 304/479 (63.47%), Postives = 369/479 (77.04%), Query Frame = 0


Query: 64  KLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAY 123
           K+ P + R +SGR   LERFSHYVARQLG  D +E PQLCKLAN YL KTKG +E +  Y
Sbjct: 61  KIAPLIERKDSGRRSNLERFSHYVARQLGFEDPNEYPQLCKLANGYLLKTKGYDENVDEY 120

Query: 124 FASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHK--RLKDLVVAAT 183
             +E E +SLYV L+EEFD+CIL+YF+F+W+Q+S +I+Q L  +S+ K  +LKD V+AAT
Sbjct: 121 LENEAERDSLYVHLLEEFDRCILTYFSFNWTQSSNLISQALSDESDQKVPKLKDFVMAAT 180

Query: 184 RKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPV-QRERSPVLLLMGG 243
           RKQRF+RV+KDLKV RV STLVEEM+ IG   S  E  C+  + PV   +RSPVLLLMGG
Sbjct: 181 RKQRFERVTKDLKVKRVISTLVEEMRVIGSGSS--EPHCTEVMSPVAHNKRSPVLLLMGG 240

Query: 244 GMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELV 303
           GMGAGKSTVLKDI  E FW EA  + V +EADAFKETDVIY+A+SS G+HDDMLQTAELV
Sbjct: 241 GMGAGKSTVLKDIFLESFWSEAQADAVVIEADAFKETDVIYRALSSRGHHDDMLQTAELV 300

Query: 304 HQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKV-EN 363
           HQSS DAASSLLVTALN+GRDVI+D TLSW+P+V Q IEMARNVHK++YRMG GYKV E 
Sbjct: 301 HQSSTDAASSLLVTALNDGRDVIMDGTLSWEPFVEQMIEMARNVHKQKYRMGEGYKVSEE 360

Query: 364 GNITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAV 423
           G ITE YW    EEEEEE +E+G+  N  PYRIELVGVVCDA+LAV RGIRRA+M+ RAV
Sbjct: 361 GTITEKYW----EEEEEETKENGKQQNLKPYRIELVGVVCDAYLAVARGIRRALMVKRAV 420

Query: 424 RVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHA 483
           RV  QL SHKRFANAFPKY E+VD+ RLY TN++G PP+L+  KDG+     +VDPE   
Sbjct: 421 RVKPQLNSHKRFANAFPKYCELVDNARLYCTNAVGGPPRLIAWKDGNS--KLLVDPEDID 480

Query: 484 YLSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSR 539
            L  +S+LN  AES+YELYP+PS   +P SVW ++ + PSR   QKEL  AI+++E ++
Sbjct: 481 CLKRVSSLNPDAESIYELYPDPSQLSKPGSVWNDVVLVPSRPKVQKELSDAIRRIEKAQ 531

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BLAST of MC05g0475 vs. NCBI nr
Match: XP_022147526.1 (uncharacterized protein LOC111016429 [Momordica charantia])

HSP 1 Score: 1084 bits (2804), Expect = 0.0
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0


Query: 1   MNKESKILIFFLRSLCFFDFFIMKHQDGGYGKVIPSSHFLTHSFAAAGKRHYRRTSNGVT 60
           MNKESKILIFFLRSLCFFDFFIMKHQDGGYGKVIPSSHFLTHSFAAAGKRHYRRTSNGVT
Sbjct: 1   MNKESKILIFFLRSLCFFDFFIMKHQDGGYGKVIPSSHFLTHSFAAAGKRHYRRTSNGVT 60

Query: 61  SYQKLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEI 120
           SYQKLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEI
Sbjct: 61  SYQKLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEI 120

Query: 121 YAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAA 180
           YAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAA
Sbjct: 121 YAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAA 180

Query: 181 TRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPVQRERSPVLLLMGG 240
           TRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPVQRERSPVLLLMGG
Sbjct: 181 TRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPVQRERSPVLLLMGG 240

Query: 241 GMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELV 300
           GMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELV
Sbjct: 241 GMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELV 300

Query: 301 HQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENG 360
           HQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENG
Sbjct: 301 HQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENG 360

Query: 361 NITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVR 420
           NITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVR
Sbjct: 361 NITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVR 420

Query: 421 VNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAY 480
           VNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAY
Sbjct: 421 VNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAY 480

Query: 481 LSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSI 540
           LSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSI
Sbjct: 481 LSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSI 540

Query: 541 LDETVQ 546
           LDETVQ
Sbjct: 541 LDETVQ 546

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BLAST of MC05g0475 vs. NCBI nr
Match: KAG6582355.1 (Calmodulin calcium-dependent NAD kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 456/530 (86.04%), Postives = 483/530 (91.13%), Query Frame = 0


Query: 23  MKHQDGGYGKVIPSSHFLTHSFA----AAGKRHYRRTSNGVTSYQKLVPHLHRTESGRVD 82
           MKH+DGG+GKVI SSH+L  SF     AAG RH+RRTS  V + QKL+PHLHRTESGRVD
Sbjct: 1   MKHEDGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLIPHLHRTESGRVD 60

Query: 83  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEVEAESLYVKLM 142
           FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASE + ESLYVKLM
Sbjct: 61  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLM 120

Query: 143 EEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAATRKQRFDRVSKDLKVTRV 202
           +EFDQCILSYFAFHWSQASLMITQVLGVDSEHK+LKDLVVAATRKQRFDRVSKDLKVTRV
Sbjct: 121 DEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRV 180

Query: 203 FSTLVEEMKKIGCAPSKGESRCSHTIPPV-QRERSPVLLLMGGGMGAGKSTVLKDISKEP 262
           FSTLVEEMKKIGCA SKGES+CSH  P + QR+RSPVLLLMGGGMGAGKSTVLKDI KEP
Sbjct: 181 FSTLVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEP 240

Query: 263 FWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQSSIDAASSLLVTALN 322
           FWLEA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQ SIDAASSLLVTALN
Sbjct: 241 FWLEAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALN 300

Query: 323 EGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENGNITENYWEPVSEEEEEE 382
           EGRDVILDSTLSW+PYVMQTIEMARN+HKRRYRMGVGYKVE  N+TENYWEPVSEEEEEE
Sbjct: 301 EGRDVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVEKDNVTENYWEPVSEEEEEE 360

Query: 383 LQ-EDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP 442
           L+ E GEM NRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP
Sbjct: 361 LRKEHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP 420

Query: 443 KYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAYLSTLSNLNAAAESVYE 502
           KYSEVVDSVRLYSTNS G PPKL+ RK+GDD P QM DPEA+A LSTLSNLN  AESVYE
Sbjct: 421 KYSEVVDSVRLYSTNSFGKPPKLIHRKEGDD-PFQMFDPEANACLSTLSNLNPDAESVYE 480

Query: 503 LYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSILDETVQ 546
           LYPNPS F EPE++WKEI  +PSR+HSQK+LRS I+K+ESSR I +E VQ
Sbjct: 481 LYPNPSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKVESSRRISNEIVQ 529

[Close]

BLAST of MC05g0475 vs. NCBI nr
Match: XP_022980774.1 (uncharacterized protein LOC111480063 [Cucurbita maxima])

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 455/530 (85.85%), Postives = 482/530 (90.94%), Query Frame = 0


Query: 23  MKHQDGGYGKVIPSSHFLTHSFA----AAGKRHYRRTSNGVTSYQKLVPHLHRTESGRVD 82
           MKH+DGG+GKVI SSH+L  SF     AAG RH+RRTS  V + QKL+PHLHRTESGRVD
Sbjct: 1   MKHEDGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLIPHLHRTESGRVD 60

Query: 83  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEVEAESLYVKLM 142
           FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASE + ESLYVKLM
Sbjct: 61  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLM 120

Query: 143 EEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAATRKQRFDRVSKDLKVTRV 202
           +EFDQCILSYFAFHWSQASLMITQVLGVDSEHK+LKDLVVAATRKQRFDRVSKDLKVTRV
Sbjct: 121 DEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRV 180

Query: 203 FSTLVEEMKKIGCAPSKGESRCSHTIPPV-QRERSPVLLLMGGGMGAGKSTVLKDISKEP 262
           FSTLVEEMKKIGCA SKGES+CSH  P + QR+RSPVLLLMGGGMGAGKSTVLKDI KEP
Sbjct: 181 FSTLVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEP 240

Query: 263 FWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQSSIDAASSLLVTALN 322
           FWLEA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQ SIDAASSLLVTALN
Sbjct: 241 FWLEAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALN 300

Query: 323 EGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENGNITENYWEPVSEEEEEE 382
           EGRDVILDSTLSW+PYVMQTIEMARN+HKRRYRMGVGYKVE  N+TENYWEPVSEEEEEE
Sbjct: 301 EGRDVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVEKDNVTENYWEPVSEEEEEE 360

Query: 383 LQ-EDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP 442
           L+ E GEM NRMPYRIELVGVVCDAHLAVVRGIRRAIM+GRAVRVNSQLQSHKRFANAFP
Sbjct: 361 LRNEHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMVGRAVRVNSQLQSHKRFANAFP 420

Query: 443 KYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAYLSTLSNLNAAAESVYE 502
           KYSEVVDSVRLYSTNS G PPKL+ RK+GDD P QM DPEA+A LSTLS LN  AESVYE
Sbjct: 421 KYSEVVDSVRLYSTNSFGKPPKLIHRKEGDD-PFQMFDPEANACLSTLSKLNPDAESVYE 480

Query: 503 LYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSILDETVQ 546
           LYPNPS F EPE++WKEI  +PSR+HSQK+LRS I+KLESSR I +E VQ
Sbjct: 481 LYPNPSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKLESSRRICNEIVQ 529

[Close]

BLAST of MC05g0475 vs. NCBI nr
Match: XP_022924243.1 (uncharacterized protein LOC111431771 [Cucurbita moschata])

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 455/530 (85.85%), Postives = 482/530 (90.94%), Query Frame = 0


Query: 23  MKHQDGGYGKVIPSSHFLTHSFA----AAGKRHYRRTSNGVTSYQKLVPHLHRTESGRVD 82
           MKH+DGG+GKVI SSH+L  SF     AAG RH+RRTS  V + QKL+PHLHRTESGRVD
Sbjct: 1   MKHEDGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLIPHLHRTESGRVD 60

Query: 83  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEVEAESLYVKLM 142
           FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASE + ESLYVKLM
Sbjct: 61  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLM 120

Query: 143 EEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAATRKQRFDRVSKDLKVTRV 202
           +EFDQCILSYFAFHWSQASLMITQVLGVDSEHK+LKDLVVAATRKQRFDRVSKDLKVTRV
Sbjct: 121 DEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRV 180

Query: 203 FSTLVEEMKKIGCAPSKGESRCSHTIPPV-QRERSPVLLLMGGGMGAGKSTVLKDISKEP 262
           FSTLVEEMKKIGCA SKGES+CSH  P + QR+RSPVLLLMGGGMGAGKSTVLKDI KEP
Sbjct: 181 FSTLVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEP 240

Query: 263 FWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQSSIDAASSLLVTALN 322
           FWLEA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQ SIDAASSLLVTALN
Sbjct: 241 FWLEAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALN 300

Query: 323 EGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENGNITENYWEPVSEEEEEE 382
           EGRDVILDSTLSW+PYVMQTIEMARN+HKRRYRMGVGYKVE  N+TENYWEPVSEEEEEE
Sbjct: 301 EGRDVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVEKDNVTENYWEPVSEEEEEE 360

Query: 383 LQ-EDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP 442
           L+ E GEM NRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP
Sbjct: 361 LRKEHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP 420

Query: 443 KYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAYLSTLSNLNAAAESVYE 502
           KYSEVVDSVRLYSTNS G PPKL+ RK+GDD P QM DPEA+A LSTLSNLN  AESVYE
Sbjct: 421 KYSEVVDSVRLYSTNSFGKPPKLIHRKEGDD-PFQMFDPEANACLSTLSNLNPDAESVYE 480

Query: 503 LYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSILDETVQ 546
           LYPN S F EPE++WKEI  +PSR+HSQK+LRS I+K+ESSR I +E VQ
Sbjct: 481 LYPNLSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKVESSRRISNEIVQ 529

[Close]

BLAST of MC05g0475 vs. NCBI nr
Match: XP_011650947.2 (uncharacterized protein LOC101217085 [Cucumis sativus] >KGN56910.1 hypothetical protein Csa_009657 [Cucumis sativus])

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 455/544 (83.64%), Postives = 485/544 (89.15%), Query Frame = 0


Query: 2   NKESKILIFFLRSLCFFDFFIMKHQDGGYGKVIPSSHFLTHSF----AAAGKRHYRRTSN 61
           +  S I   FLRSLC  + FIMKH+DGG+GK+IP+SH+L  SF    AA G RH+RRTS 
Sbjct: 9   SNNSLIFFLFLRSLCSSNLFIMKHEDGGFGKLIPTSHYLNQSFVGGVAATGNRHFRRTSK 68

Query: 62  GVTSYQKLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCE 121
            V + QKL+PHLHRTESGRVDFLERFSHYVARQLGISD DECPQLCKLANNYLRKTKGCE
Sbjct: 69  PVINNQKLIPHLHRTESGRVDFLERFSHYVARQLGISDVDECPQLCKLANNYLRKTKGCE 128

Query: 122 EEIYAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLV 181
           EEIYAYFASE   ESLYVKL+ EFDQCILSYFAFHWSQASLMITQVLGVDSEHK+LKDLV
Sbjct: 129 EEIYAYFASEAGGESLYVKLVTEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLV 188

Query: 182 VAATRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIP-PVQRERSPVLL 241
           VAATRKQRFDRVSKDLK+TRVFSTLVEEMKKIGCA SKGES+CS T P   QR+RSPVLL
Sbjct: 189 VAATRKQRFDRVSKDLKMTRVFSTLVEEMKKIGCAASKGESKCSLTKPRSSQRQRSPVLL 248

Query: 242 LMGGGMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQT 301
           +MGGGMGAGKSTVLKDI KEPFWLEA TN V VEADAFKETDVIYKAISSMGYHDDMLQT
Sbjct: 249 MMGGGMGAGKSTVLKDILKEPFWLEAETNTVTVEADAFKETDVIYKAISSMGYHDDMLQT 308

Query: 302 AELVHQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYK 361
           AELVHQ SIDAASSLLVTALNEGRDVILDSTLSW PYVMQTIEMARN+HKRRYRMGVGYK
Sbjct: 309 AELVHQPSIDAASSLLVTALNEGRDVILDSTLSWAPYVMQTIEMARNIHKRRYRMGVGYK 368

Query: 362 VENGNITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMG 421
           VENG +TENYWEPVSEEEE     DGEM +RMPYRIELVGVVCDAHLAVVRGIRRAIMMG
Sbjct: 369 VENGEVTENYWEPVSEEEE-----DGEMQDRMPYRIELVGVVCDAHLAVVRGIRRAIMMG 428

Query: 422 RAVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPE 481
           RAVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTN IGNPPKL+ RKDG D P Q +D E
Sbjct: 429 RAVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNFIGNPPKLIHRKDGTD-PFQTIDAE 488

Query: 482 AHAYLSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLES 540
           A A L+TLSNLN  AESVYELYPNPSPF EPE++WKEI ++PSR+ SQKELRSAI+KLES
Sbjct: 489 ASACLTTLSNLNPDAESVYELYPNPSPFSEPEAIWKEIALTPSRLQSQKELRSAIKKLES 546

[Close]

BLAST of MC05g0475 vs. ExPASy TrEMBL
Match: A0A6J1D2M7 (uncharacterized protein LOC111016429 OS=Momordica charantia OX=3673 GN=LOC111016429 PE=4 SV=1)

HSP 1 Score: 1084 bits (2804), Expect = 0.0
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0


Query: 1   MNKESKILIFFLRSLCFFDFFIMKHQDGGYGKVIPSSHFLTHSFAAAGKRHYRRTSNGVT 60
           MNKESKILIFFLRSLCFFDFFIMKHQDGGYGKVIPSSHFLTHSFAAAGKRHYRRTSNGVT
Sbjct: 1   MNKESKILIFFLRSLCFFDFFIMKHQDGGYGKVIPSSHFLTHSFAAAGKRHYRRTSNGVT 60

Query: 61  SYQKLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEI 120
           SYQKLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEI
Sbjct: 61  SYQKLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEI 120

Query: 121 YAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAA 180
           YAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAA
Sbjct: 121 YAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAA 180

Query: 181 TRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPVQRERSPVLLLMGG 240
           TRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPVQRERSPVLLLMGG
Sbjct: 181 TRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPVQRERSPVLLLMGG 240

Query: 241 GMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELV 300
           GMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELV
Sbjct: 241 GMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELV 300

Query: 301 HQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENG 360
           HQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENG
Sbjct: 301 HQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENG 360

Query: 361 NITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVR 420
           NITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVR
Sbjct: 361 NITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVR 420

Query: 421 VNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAY 480
           VNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAY
Sbjct: 421 VNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAY 480

Query: 481 LSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSI 540
           LSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSI
Sbjct: 481 LSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSI 540

Query: 541 LDETVQ 546
           LDETVQ
Sbjct: 541 LDETVQ 546

[Close]

BLAST of MC05g0475 vs. ExPASy TrEMBL
Match: A0A6J1J066 (uncharacterized protein LOC111480063 OS=Cucurbita maxima OX=3661 GN=LOC111480063 PE=4 SV=1)

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 455/530 (85.85%), Postives = 482/530 (90.94%), Query Frame = 0


Query: 23  MKHQDGGYGKVIPSSHFLTHSFA----AAGKRHYRRTSNGVTSYQKLVPHLHRTESGRVD 82
           MKH+DGG+GKVI SSH+L  SF     AAG RH+RRTS  V + QKL+PHLHRTESGRVD
Sbjct: 1   MKHEDGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLIPHLHRTESGRVD 60

Query: 83  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEVEAESLYVKLM 142
           FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASE + ESLYVKLM
Sbjct: 61  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLM 120

Query: 143 EEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAATRKQRFDRVSKDLKVTRV 202
           +EFDQCILSYFAFHWSQASLMITQVLGVDSEHK+LKDLVVAATRKQRFDRVSKDLKVTRV
Sbjct: 121 DEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRV 180

Query: 203 FSTLVEEMKKIGCAPSKGESRCSHTIPPV-QRERSPVLLLMGGGMGAGKSTVLKDISKEP 262
           FSTLVEEMKKIGCA SKGES+CSH  P + QR+RSPVLLLMGGGMGAGKSTVLKDI KEP
Sbjct: 181 FSTLVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEP 240

Query: 263 FWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQSSIDAASSLLVTALN 322
           FWLEA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQ SIDAASSLLVTALN
Sbjct: 241 FWLEAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALN 300

Query: 323 EGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENGNITENYWEPVSEEEEEE 382
           EGRDVILDSTLSW+PYVMQTIEMARN+HKRRYRMGVGYKVE  N+TENYWEPVSEEEEEE
Sbjct: 301 EGRDVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVEKDNVTENYWEPVSEEEEEE 360

Query: 383 LQ-EDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP 442
           L+ E GEM NRMPYRIELVGVVCDAHLAVVRGIRRAIM+GRAVRVNSQLQSHKRFANAFP
Sbjct: 361 LRNEHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMVGRAVRVNSQLQSHKRFANAFP 420

Query: 443 KYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAYLSTLSNLNAAAESVYE 502
           KYSEVVDSVRLYSTNS G PPKL+ RK+GDD P QM DPEA+A LSTLS LN  AESVYE
Sbjct: 421 KYSEVVDSVRLYSTNSFGKPPKLIHRKEGDD-PFQMFDPEANACLSTLSKLNPDAESVYE 480

Query: 503 LYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSILDETVQ 546
           LYPNPS F EPE++WKEI  +PSR+HSQK+LRS I+KLESSR I +E VQ
Sbjct: 481 LYPNPSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKLESSRRICNEIVQ 529

[Close]

BLAST of MC05g0475 vs. ExPASy TrEMBL
Match: A0A6J1EBV0 (uncharacterized protein LOC111431771 OS=Cucurbita moschata OX=3662 GN=LOC111431771 PE=4 SV=1)

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 455/530 (85.85%), Postives = 482/530 (90.94%), Query Frame = 0


Query: 23  MKHQDGGYGKVIPSSHFLTHSFA----AAGKRHYRRTSNGVTSYQKLVPHLHRTESGRVD 82
           MKH+DGG+GKVI SSH+L  SF     AAG RH+RRTS  V + QKL+PHLHRTESGRVD
Sbjct: 1   MKHEDGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLIPHLHRTESGRVD 60

Query: 83  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEVEAESLYVKLM 142
           FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASE + ESLYVKLM
Sbjct: 61  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLM 120

Query: 143 EEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAATRKQRFDRVSKDLKVTRV 202
           +EFDQCILSYFAFHWSQASLMITQVLGVDSEHK+LKDLVVAATRKQRFDRVSKDLKVTRV
Sbjct: 121 DEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRV 180

Query: 203 FSTLVEEMKKIGCAPSKGESRCSHTIPPV-QRERSPVLLLMGGGMGAGKSTVLKDISKEP 262
           FSTLVEEMKKIGCA SKGES+CSH  P + QR+RSPVLLLMGGGMGAGKSTVLKDI KEP
Sbjct: 181 FSTLVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEP 240

Query: 263 FWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQSSIDAASSLLVTALN 322
           FWLEA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQ SIDAASSLLVTALN
Sbjct: 241 FWLEAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALN 300

Query: 323 EGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENGNITENYWEPVSEEEEEE 382
           EGRDVILDSTLSW+PYVMQTIEMARN+HKRRYRMGVGYKVE  N+TENYWEPVSEEEEEE
Sbjct: 301 EGRDVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVEKDNVTENYWEPVSEEEEEE 360

Query: 383 LQ-EDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP 442
           L+ E GEM NRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP
Sbjct: 361 LRKEHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFP 420

Query: 443 KYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAYLSTLSNLNAAAESVYE 502
           KYSEVVDSVRLYSTNS G PPKL+ RK+GDD P QM DPEA+A LSTLSNLN  AESVYE
Sbjct: 421 KYSEVVDSVRLYSTNSFGKPPKLIHRKEGDD-PFQMFDPEANACLSTLSNLNPDAESVYE 480

Query: 503 LYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSILDETVQ 546
           LYPN S F EPE++WKEI  +PSR+HSQK+LRS I+K+ESSR I +E VQ
Sbjct: 481 LYPNLSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKVESSRRISNEIVQ 529

[Close]

BLAST of MC05g0475 vs. ExPASy TrEMBL
Match: A0A0A0L4J6 (Zeta_toxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G143510 PE=4 SV=1)

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 455/544 (83.64%), Postives = 485/544 (89.15%), Query Frame = 0


Query: 2   NKESKILIFFLRSLCFFDFFIMKHQDGGYGKVIPSSHFLTHSF----AAAGKRHYRRTSN 61
           +  S I   FLRSLC  + FIMKH+DGG+GK+IP+SH+L  SF    AA G RH+RRTS 
Sbjct: 9   SNNSLIFFLFLRSLCSSNLFIMKHEDGGFGKLIPTSHYLNQSFVGGVAATGNRHFRRTSK 68

Query: 62  GVTSYQKLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCE 121
            V + QKL+PHLHRTESGRVDFLERFSHYVARQLGISD DECPQLCKLANNYLRKTKGCE
Sbjct: 69  PVINNQKLIPHLHRTESGRVDFLERFSHYVARQLGISDVDECPQLCKLANNYLRKTKGCE 128

Query: 122 EEIYAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLV 181
           EEIYAYFASE   ESLYVKL+ EFDQCILSYFAFHWSQASLMITQVLGVDSEHK+LKDLV
Sbjct: 129 EEIYAYFASEAGGESLYVKLVTEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLV 188

Query: 182 VAATRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIP-PVQRERSPVLL 241
           VAATRKQRFDRVSKDLK+TRVFSTLVEEMKKIGCA SKGES+CS T P   QR+RSPVLL
Sbjct: 189 VAATRKQRFDRVSKDLKMTRVFSTLVEEMKKIGCAASKGESKCSLTKPRSSQRQRSPVLL 248

Query: 242 LMGGGMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQT 301
           +MGGGMGAGKSTVLKDI KEPFWLEA TN V VEADAFKETDVIYKAISSMGYHDDMLQT
Sbjct: 249 MMGGGMGAGKSTVLKDILKEPFWLEAETNTVTVEADAFKETDVIYKAISSMGYHDDMLQT 308

Query: 302 AELVHQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYK 361
           AELVHQ SIDAASSLLVTALNEGRDVILDSTLSW PYVMQTIEMARN+HKRRYRMGVGYK
Sbjct: 309 AELVHQPSIDAASSLLVTALNEGRDVILDSTLSWAPYVMQTIEMARNIHKRRYRMGVGYK 368

Query: 362 VENGNITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMG 421
           VENG +TENYWEPVSEEEE     DGEM +RMPYRIELVGVVCDAHLAVVRGIRRAIMMG
Sbjct: 369 VENGEVTENYWEPVSEEEE-----DGEMQDRMPYRIELVGVVCDAHLAVVRGIRRAIMMG 428

Query: 422 RAVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPE 481
           RAVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTN IGNPPKL+ RKDG D P Q +D E
Sbjct: 429 RAVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNFIGNPPKLIHRKDGTD-PFQTIDAE 488

Query: 482 AHAYLSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLES 540
           A A L+TLSNLN  AESVYELYPNPSPF EPE++WKEI ++PSR+ SQKELRSAI+KLES
Sbjct: 489 ASACLTTLSNLNPDAESVYELYPNPSPFSEPEAIWKEIALTPSRLQSQKELRSAIKKLES 546

[Close]

BLAST of MC05g0475 vs. ExPASy TrEMBL
Match: A0A5A7U1T8 (P-loop nucleoside triphosphate hydrolase superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001100 PE=4 SV=1)

HSP 1 Score: 874 bits (2257), Expect = 0.0
Identity = 448/529 (84.69%), Postives = 477/529 (90.17%), Query Frame = 0


Query: 23  MKHQDGGYGKVIPSSHFLTHSF----AAAGKRHYRRTSNGVTSYQKLVPHLHRTESGRVD 82
           MKHQDGG+GKVIP+SH+L  SF    +A G RH+RRTS  V + QKL+PHL RTESGRVD
Sbjct: 1   MKHQDGGFGKVIPTSHYLNTSFVGGVSATGNRHFRRTSKPVINNQKLIPHLRRTESGRVD 60

Query: 83  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEVEAESLYVKLM 142
           FLERFSHYVARQLGISD DECPQLCKLANNYLRKTKGCEEEIYAYFASE E ESLYVKL+
Sbjct: 61  FLERFSHYVARQLGISDVDECPQLCKLANNYLRKTKGCEEEIYAYFASEAEGESLYVKLV 120

Query: 143 EEFDQCILSYFAFHWSQASLMITQVLGVDSEHKRLKDLVVAATRKQRFDRVSKDLKVTRV 202
            EFDQCILSYFAFHWSQASLMITQVLGVDSEHK+LKDLVVAATRKQRFDRVSKDLK+TRV
Sbjct: 121 NEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKMTRV 180

Query: 203 FSTLVEEMKKIGCAPSKGESRCSHTIP-PVQRERSPVLLLMGGGMGAGKSTVLKDISKEP 262
           FSTLVEEMKKIGCA SKGES+CS T P   QR+RSPVLLLMGGGMGAGKSTVLKDI KEP
Sbjct: 181 FSTLVEEMKKIGCAASKGESKCSLTTPRSSQRQRSPVLLLMGGGMGAGKSTVLKDILKEP 240

Query: 263 FWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQSSIDAASSLLVTALN 322
           FWLEA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQ SIDAASSLLVTALN
Sbjct: 241 FWLEAETNTVKVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALN 300

Query: 323 EGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKVENGNITENYWEPVSEEEEEE 382
           EGRDVILDSTLSW+PYVMQTIEMARN+HKRRYRMGVGYKVENG +TENYWEPVSEEEE+E
Sbjct: 301 EGRDVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVENGKVTENYWEPVSEEEEDE 360

Query: 383 LQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPK 442
                EM +RMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRV+SQLQSHKRFANAFPK
Sbjct: 361 -----EMQDRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVSSQLQSHKRFANAFPK 420

Query: 443 YSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHAYLSTLSNLNAAAESVYEL 502
           YSEVVDSVRLYSTN IGNPPKL+ RKDG D P Q +D EA A L+TLSNLN  AESVYEL
Sbjct: 421 YSEVVDSVRLYSTNYIGNPPKLIHRKDGTD-PFQTIDAEASACLTTLSNLNPDAESVYEL 480

Query: 503 YPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSRSILDETVQ 546
           YPNPSPF EPE++WKEI ++PSR+HSQKELRSAI+K+ESSR+I  E  Q
Sbjct: 481 YPNPSPFSEPEAIWKEIALTPSRLHSQKELRSAIKKIESSRTISSEVEQ 523

[Close]

BLAST of MC05g0475 vs. TAIR 10
Match: AT1G04280.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 576.2 bits (1484), Expect = 2.7e-164
Identity = 304/479 (63.47%), Postives = 369/479 (77.04%), Query Frame = 0


Query: 64  KLVPHLHRTESGRVDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAY 123
           K+ P + R +SGR   LERFSHYVARQLG  D +E PQLCKLAN YL KTKG +E +  Y
Sbjct: 61  KIAPLIERKDSGRRSNLERFSHYVARQLGFEDPNEYPQLCKLANGYLLKTKGYDENVDEY 120

Query: 124 FASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHK--RLKDLVVAAT 183
             +E E +SLYV L+EEFD+CIL+YF+F+W+Q+S +I+Q L  +S+ K  +LKD V+AAT
Sbjct: 121 LENEAERDSLYVHLLEEFDRCILTYFSFNWTQSSNLISQALSDESDQKVPKLKDFVMAAT 180

Query: 184 RKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPV-QRERSPVLLLMGG 243
           RKQRF+RV+KDLKV RV STLVEEM+ IG   S  E  C+  + PV   +RSPVLLLMGG
Sbjct: 181 RKQRFERVTKDLKVKRVISTLVEEMRVIGSGSS--EPHCTEVMSPVAHNKRSPVLLLMGG 240

Query: 244 GMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELV 303
           GMGAGKSTVLKDI  E FW EA  + V +EADAFKETDVIY+A+SS G+HDDMLQTAELV
Sbjct: 241 GMGAGKSTVLKDIFLESFWSEAQADAVVIEADAFKETDVIYRALSSRGHHDDMLQTAELV 300

Query: 304 HQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKV-EN 363
           HQSS DAASSLLVTALN+GRDVI+D TLSW+P+V Q IEMARNVHK++YRMG GYKV E 
Sbjct: 301 HQSSTDAASSLLVTALNDGRDVIMDGTLSWEPFVEQMIEMARNVHKQKYRMGEGYKVSEE 360

Query: 364 GNITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAV 423
           G ITE YW    EEEEEE +E+G+  N  PYRIELVGVVCDA+LAV RGIRRA+M+ RAV
Sbjct: 361 GTITEKYW----EEEEEETKENGKQQNLKPYRIELVGVVCDAYLAVARGIRRALMVKRAV 420

Query: 424 RVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHA 483
           RV  QL SHKRFANAFPKY E+VD+ RLY TN++G PP+L+  KDG+     +VDPE   
Sbjct: 421 RVKPQLNSHKRFANAFPKYCELVDNARLYCTNAVGGPPRLIAWKDGNS--KLLVDPEDID 480

Query: 484 YLSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESSR 539
            L  +S+LN  AES+YELYP+PS   +P SVW ++ + PSR   QKEL  AI+++E ++
Sbjct: 481 CLKRVSSLNPDAESIYELYPDPSQLSKPGSVWNDVVLVPSRPKVQKELSDAIRRIEKAQ 531

[Close]

BLAST of MC05g0475 vs. TAIR 10
Match: AT2G30630.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 553.9 bits (1426), Expect = 1.4e-157
Identity = 295/487 (60.57%), Postives = 373/487 (76.59%), Query Frame = 0


Query: 62  YQKLVPHLHRTESGR-VDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEI 121
           + K +P L  ++  +  + LERFSHYV RQ+G  D  ECP LCKLAN Y+RK+  CEE+I
Sbjct: 53  HSKNIPRLRSSQKHKGHEKLERFSHYVVRQMGFKDRRECPHLCKLANEYIRKSGSCEEDI 112

Query: 122 YAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSE-HKRLKDLVVA 181
           Y++F+ E  A+SL++KL+EEF++CILSYFA+HWS A LMI+Q+L  D E  K+LK +V+A
Sbjct: 113 YSFFSEEPGADSLFIKLVEEFERCILSYFAYHWSHADLMISQILSADVEPKKKLKHIVMA 172

Query: 182 ATRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPV-QRERSPVLLLM 241
           ATR+QRF+RV+K+LKV RVF+TLVEEMK +G A S  +S C+  + PV  ++RSPVLLLM
Sbjct: 173 ATREQRFERVTKNLKVARVFNTLVEEMKAMGIA-SVDDSECTEVMAPVAHKDRSPVLLLM 232

Query: 242 GGGMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAE 301
           GGGMGAGKSTVLKDI KEPFW  A  + V +EADAFKE+DVIY+A+S+ G H DM++TAE
Sbjct: 233 GGGMGAGKSTVLKDILKEPFW--AGADAVVIEADAFKESDVIYRALSARG-HVDMIKTAE 292

Query: 302 LVHQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKV- 361
            VHQSS DAASSLLVTALNEGRDVI+D TLSW P+V+QTI MARNVH+ RYRMG GYKV 
Sbjct: 293 FVHQSSTDAASSLLVTALNEGRDVIMDGTLSWVPFVVQTITMARNVHRHRYRMGAGYKVG 352

Query: 362 ENGNITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGR 421
           ENG++ ENYWE +   E ++LQEDG    R PYRIELVGVVCDA+LAV+RGIRRAIM  R
Sbjct: 353 ENGDVIENYWERIG--ERQQLQEDG--RERKPYRIELVGVVCDAYLAVIRGIRRAIMCRR 412

Query: 422 AVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEA 481
           AVRV SQL+SHKRFANAF  Y  +VD+ RLY TN++   PKL+  K  + + + +VDPE 
Sbjct: 413 AVRVRSQLRSHKRFANAFLTYCNLVDNARLYCTNALEGSPKLIGWK--EKEKTLLVDPEE 472

Query: 482 HAYLSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLESS 541
              L  +  LN  A+S+YELY NP+P  E  S+WK+I +SPSR + Q+EL+ +IQK+E  
Sbjct: 473 IDCLKNVGRLNENADSIYELYRNPNPACEAGSIWKDIVLSPSRFNIQQELKYSIQKVERF 529

Query: 542 RSILDET 545
           +  L ET
Sbjct: 533 KQYLQET 529

[Close]

BLAST of MC05g0475 vs. TAIR 10
Match: AT2G30630.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 542.0 bits (1395), Expect = 5.6e-154
Identity = 296/508 (58.27%), Postives = 373/508 (73.43%), Query Frame = 0


Query: 62  YQKLVPHLHRTESGR-VDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEI 121
           + K +P L  ++  +  + LERFSHYV RQ+G  D  ECP LCKLAN Y+RK+  CEE+I
Sbjct: 53  HSKNIPRLRSSQKHKGHEKLERFSHYVVRQMGFKDRRECPHLCKLANEYIRKSGSCEEDI 112

Query: 122 YAYFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQV----------------- 181
           Y++F+ E  A+SL++KL+EEF++CILSYFA+HWS A LMI+QV                 
Sbjct: 113 YSFFSEEPGADSLFIKLVEEFERCILSYFAYHWSHADLMISQVTNKLSLSLLCYITNRPK 172

Query: 182 ----LGVDSE-HKRLKDLVVAATRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGES 241
               L  D E  K+LK +V+AATR+QRF+RV+K+LKV RVF+TLVEEMK +G A S  +S
Sbjct: 173 TFQILSADVEPKKKLKHIVMAATREQRFERVTKNLKVARVFNTLVEEMKAMGIA-SVDDS 232

Query: 242 RCSHTIPPV-QRERSPVLLLMGGGMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKET 301
            C+  + PV  ++RSPVLLLMGGGMGAGKSTVLKDI KEPFW  A  + V +EADAFKE+
Sbjct: 233 ECTEVMAPVAHKDRSPVLLLMGGGMGAGKSTVLKDILKEPFW--AGADAVVIEADAFKES 292

Query: 302 DVIYKAISSMGYHDDMLQTAELVHQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQT 361
           DVIY+A+S+ G H DM++TAE VHQSS DAASSLLVTALNEGRDVI+D TLSW P+V+QT
Sbjct: 293 DVIYRALSARG-HVDMIKTAEFVHQSSTDAASSLLVTALNEGRDVIMDGTLSWVPFVVQT 352

Query: 362 IEMARNVHKRRYRMGVGYKV-ENGNITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVG 421
           I MARNVH+ RYRMG GYKV ENG++ ENYWE +   E ++LQEDG    R PYRIELVG
Sbjct: 353 ITMARNVHRHRYRMGAGYKVGENGDVIENYWERIG--ERQQLQEDG--RERKPYRIELVG 412

Query: 422 VVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNP 481
           VVCDA+LAV+RGIRRAIM  RAVRV SQL+SHKRFANAF  Y  +VD+ RLY TN++   
Sbjct: 413 VVCDAYLAVIRGIRRAIMCRRAVRVRSQLRSHKRFANAFLTYCNLVDNARLYCTNALEGS 472

Query: 482 PKLLIRKDGDDDPSQMVDPEAHAYLSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGV 541
           PKL+  K  + + + +VDPE    L  +  LN  A+S+YELY NP+P  E  S+WK+I +
Sbjct: 473 PKLIGWK--EKEKTLLVDPEEIDCLKNVGRLNENADSIYELYRNPNPACEAGSIWKDIVL 532

Query: 542 SPSRIHSQKELRSAIQKLESSRSILDET 545
           SPSR + Q+EL+ +IQK+E  +  L ET
Sbjct: 533 SPSRFNIQQELKYSIQKVERFKQYLQET 550

[Close]

BLAST of MC05g0475 vs. TAIR 10
Match: AT1G06750.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 534.6 bits (1376), Expect = 9.0e-152
Identity = 291/487 (59.75%), Postives = 369/487 (75.77%), Query Frame = 0


Query: 64  KLVPHLHRTESGR-VDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYA 123
           K++P L  +   +  + LERFSHYVARQ+G  D  ECP LCKLA  Y+ K++ CEE+IY+
Sbjct: 41  KIIPRLKSSHKHKGHEKLERFSHYVARQMGFKDRRECPNLCKLAAEYISKSECCEEDIYS 100

Query: 124 YFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKR-LKDLVVAAT 183
           +F+ E +A++L++KL+EEF++CILSYFAFHWS A LMITQVL  D+E KR LK +V+AAT
Sbjct: 101 FFSEEPDADTLFIKLVEEFERCILSYFAFHWSHADLMITQVLSADAEPKRKLKQIVMAAT 160

Query: 184 RKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPV-QRERSPVLLLMGG 243
           R+ R  RV+K+LKV RVF+TLVEEM+ +G A S  +S+C+  + PV  ++RSPVLLLMGG
Sbjct: 161 RELRIKRVTKNLKVARVFNTLVEEMRAMGLA-SVDDSQCTEVMAPVAHKDRSPVLLLMGG 220

Query: 244 GMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELV 303
           GMGAGKSTVLKDI KE FW  A  + V +EADAFKE+DVIY+A+SS G H DM+QTAELV
Sbjct: 221 GMGAGKSTVLKDILKEAFW--AGADSVVIEADAFKESDVIYRALSSRG-HVDMIQTAELV 280

Query: 304 HQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKV-EN 363
           HQSS DAASSLLVTALNEGRDVI+D TLSW P+V+QTI MARNVH+  YRMG GYKV ++
Sbjct: 281 HQSSTDAASSLLVTALNEGRDVIMDGTLSWVPFVVQTITMARNVHRHHYRMGAGYKVGDD 340

Query: 364 GNITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAV 423
           G ITENYWE +   E +++QEDG    R PYRIELVGVVCDA+LAV+RGIRRAIM  RAV
Sbjct: 341 GVITENYWERIG--ERQQIQEDG--RRRKPYRIELVGVVCDAYLAVIRGIRRAIMCRRAV 400

Query: 424 RVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHA 483
           RV SQL+SHKRFA AFP Y  +VD+ RLY TN++   PKL+  K  + + + +VD E   
Sbjct: 401 RVRSQLRSHKRFAEAFPTYCSLVDNARLYCTNALEGSPKLIGWK--EKEKTLLVDTEEID 460

Query: 484 YLSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLE--SS 543
            L  +  LN  A S+YELY  P+P  E  S+WK+I +SPSR   Q+EL+ +IQK+E  S 
Sbjct: 461 CLKRVGGLNENAGSIYELYRQPNPACEAGSIWKDIVLSPSRFSIQQELKYSIQKVEKRSK 517

Query: 544 RSILDET 545
           + +++ T
Sbjct: 521 QHLINNT 517

[Close]

BLAST of MC05g0475 vs. TAIR 10
Match: AT1G06750.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 534.6 bits (1376), Expect = 9.0e-152
Identity = 291/487 (59.75%), Postives = 369/487 (75.77%), Query Frame = 0


Query: 64  KLVPHLHRTESGR-VDFLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYA 123
           K++P L  +   +  + LERFSHYVARQ+G  D  ECP LCKLA  Y+ K++ CEE+IY+
Sbjct: 48  KIIPRLKSSHKHKGHEKLERFSHYVARQMGFKDRRECPNLCKLAAEYISKSECCEEDIYS 107

Query: 124 YFASEVEAESLYVKLMEEFDQCILSYFAFHWSQASLMITQVLGVDSEHKR-LKDLVVAAT 183
           +F+ E +A++L++KL+EEF++CILSYFAFHWS A LMITQVL  D+E KR LK +V+AAT
Sbjct: 108 FFSEEPDADTLFIKLVEEFERCILSYFAFHWSHADLMITQVLSADAEPKRKLKQIVMAAT 167

Query: 184 RKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESRCSHTIPPV-QRERSPVLLLMGG 243
           R+ R  RV+K+LKV RVF+TLVEEM+ +G A S  +S+C+  + PV  ++RSPVLLLMGG
Sbjct: 168 RELRIKRVTKNLKVARVFNTLVEEMRAMGLA-SVDDSQCTEVMAPVAHKDRSPVLLLMGG 227

Query: 244 GMGAGKSTVLKDISKEPFWLEAATNPVFVEADAFKETDVIYKAISSMGYHDDMLQTAELV 303
           GMGAGKSTVLKDI KE FW  A  + V +EADAFKE+DVIY+A+SS G H DM+QTAELV
Sbjct: 228 GMGAGKSTVLKDILKEAFW--AGADSVVIEADAFKESDVIYRALSSRG-HVDMIQTAELV 287

Query: 304 HQSSIDAASSLLVTALNEGRDVILDSTLSWDPYVMQTIEMARNVHKRRYRMGVGYKV-EN 363
           HQSS DAASSLLVTALNEGRDVI+D TLSW P+V+QTI MARNVH+  YRMG GYKV ++
Sbjct: 288 HQSSTDAASSLLVTALNEGRDVIMDGTLSWVPFVVQTITMARNVHRHHYRMGAGYKVGDD 347

Query: 364 GNITENYWEPVSEEEEEELQEDGEMNNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAV 423
           G ITENYWE +   E +++QEDG    R PYRIELVGVVCDA+LAV+RGIRRAIM  RAV
Sbjct: 348 GVITENYWERIG--ERQQIQEDG--RRRKPYRIELVGVVCDAYLAVIRGIRRAIMCRRAV 407

Query: 424 RVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLLIRKDGDDDPSQMVDPEAHA 483
           RV SQL+SHKRFA AFP Y  +VD+ RLY TN++   PKL+  K  + + + +VD E   
Sbjct: 408 RVRSQLRSHKRFAEAFPTYCSLVDNARLYCTNALEGSPKLIGWK--EKEKTLLVDTEEID 467

Query: 484 YLSTLSNLNAAAESVYELYPNPSPFVEPESVWKEIGVSPSRIHSQKELRSAIQKLE--SS 543
            L  +  LN  A S+YELY  P+P  E  S+WK+I +SPSR   Q+EL+ +IQK+E  S 
Sbjct: 468 CLKRVGGLNENAGSIYELYRQPNPACEAGSIWKDIVLSPSRFSIQQELKYSIQKVEKRSK 524

Query: 544 RSILDET 545
           + +++ T
Sbjct: 528 QHLINNT 524

The following BLAST results are available for this feature:

BLAST of MC05g0475 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WUY1	3.8e-163	63.47	Calmodulin calcium-dependent NAD kinase OS=Arabidopsis thaliana OX=3702 GN=NADKC...	[more]

BLAST of MC05g0475 vs. NCBI nr


Match Name	Identity	Description
XP_022147526.1	100.00	uncharacterized protein LOC111016429 [Momordica charantia]	[more]
KAG6582355.1	86.04	Calmodulin calcium-dependent NAD kinase, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022980774.1	85.85	uncharacterized protein LOC111480063 [Cucurbita maxima]	[more]
XP_022924243.1	85.85	uncharacterized protein LOC111431771 [Cucurbita moschata]	[more]
XP_011650947.2	83.64	uncharacterized protein LOC101217085 [Cucumis sativus] >KGN56910.1 hypothetical ...	[more]

BLAST of MC05g0475 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1D2M7	100.00	uncharacterized protein LOC111016429 OS=Momordica charantia OX=3673 GN=LOC111016...	[more]
A0A6J1J066	85.85	uncharacterized protein LOC111480063 OS=Cucurbita maxima OX=3661 GN=LOC111480063...	[more]
A0A6J1EBV0	85.85	uncharacterized protein LOC111431771 OS=Cucurbita moschata OX=3662 GN=LOC1114317...	[more]
A0A0A0L4J6	83.64	Zeta_toxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G143510 ...	[more]
A0A5A7U1T8	84.69	P-loop nucleoside triphosphate hydrolase superfamily protein OS=Cucumis melo var...	[more]

BLAST of MC05g0475 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G04280.1	2.7e-164	63.47	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT2G30630.1	1.4e-157	60.57	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT2G30630.2	5.6e-154	58.27	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT1G06750.1	9.0e-152	59.75	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT1G06750.2	9.0e-152	59.75	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010488	Zeta toxin domain	PFAM	PF06414	Zeta_toxin	coord: 227..342 e-value: 1.4E-13 score: 50.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 217..458 e-value: 4.0E-26 score: 93.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 231..358
IPR044802	Calmodulin calcium-dependent NAD kinase NADKc-like	PANTHER	PTHR31153	UNCHARACTERIZED	coord: 61..539
None	No IPR available	PANTHER	PTHR31153:SF13	P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN	coord: 61..539

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0475.1	MC05g0475.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0016301	kinase activity