MC05g0444 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0444
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pyridoxal 5'-phosphate synthase
Location	MC05: 3294355 .. 3296806 (+)
RNA-Seq Expression	MC05g0444
Synteny	MC05g0444

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAAAATTGATATCCGATATTAATTACTGAAAAGTTGAACTTCGTTCCTGAAAGTACTATTTCTCAGTGATGCACCGAACGGAAATGGGAAATTGAAACCATTAGGCAAATTAGCGAGCGGACAATTGGGAGATGGGATCGGCAGCAGCGCCATGGAAGTCCCTTCTTCTCACTGCTTTGGAATCTAATGGCCACATCAAGCATTCCCAATTCTTGCAGCTCGTACAGCTCTCTCACTATTTCTCTCAAAGCTTAACTACTTTTTTTTTTTTTTTCCTTTCTCACCGTTTCGATTTCGAATTCGTTTTCAGGCAACGGTAGGAGCGAATGGAAGGCCCTCCAATCGCACAGTTGTTTTCAGAGGATTCCAAGAGGGCAGCGATAATATCCAAATCAACACGGATTCTAGAAGTCGCAAGGTTCTTTTACGGGAAGCCTTATATTTCATTTTAAATTGCTTAATTCATACGGCCATTTGTTGTTCTGTGGTTTTTAACGCAATGATTATCTGGGTTTCTGGAATTTTATTGCAATCTGTATTTAGTGTTATTGATTATGTAATGTGGATCCACGTCTATGGCAGTAACAAGACAAGCGATGAATTATGCTCGGTTCCTAACAGGCATTTTCTGGTTCTGTAATCGCGTCTATTGACTCTCTCGATACTGTTGACTCCTCGACCATCCAAGTATCAACAATAGCTTGGATCTTCTAGAACAGAAATATAGCAGATTTTCCTAGTAGAACTTCTGCATTGATTTGTTGGCTGGCAATTTGTTTGTGATGAAGTAGATCTGAGTTTTGTCTTTAAAATGAACTTGGCTTTTGGTTGATTCTGCAGGTTGAAGAGCTTAAGAATTGTCCATTTGCTGAGGTATTAATCTCCCTGGCTAGAGAACATAGTATATGATATAAATAATTTCTTCATGAGTCTCTCTAGCTTATTGTATTGAGATTGCTGCTGAAATGTATGTGCCAATTTGAGTCAAAATTTCGATTTTAAATGTTTTAGTCTTTTAGATATGCTGGTATTTCACCGAATCATGGGAGCAATTTCGGATCAGTGGGAGAGTTGAGGTTGTTGATTCATTGTATCCTGATGCTATAAAACTTCAGGTTGTAGGCATGGCCCTTTCCACTTGTAACTTGAAGCTGGTTCTAACTTTTTAGATTTCACTTTTTCACTTTACTCTTGGTGATATTCTCTTGCATATTCTATCATGATCATGCTGAGTTCACATTGCAGCAAAGAGAGAAAGCATGGTCCTCTATTCCTTCAAAGACAAGAATGCAGTATTTGGGACCTAATCCAGGCCTTCCCTATCTTACTGGACAACCAGCCAAGGAAAATCTTCAAGATTCTTCTTCGGGTCCTGTTGATTCATACTGTTTGCTTGTTTTCAATCCTGATCAGGTTTGTAATGTATTTTGTACATGATCATACATTCTCCAATCTCCATCATGTTAATTTTCCATATGTTACTTACCCCTTTCTATCTTTCATTCACAGGTTGATTATGTGAATTTGAAGAGCGAAAAAAGGTTTTTGTATAAAGCGAGTTCGAGTTCAAGAGGGGAGAAGCTGTGGAGTACTGAGAGGATAAACCCTTAGCCACCGACAACGAGCTGCTATCCTAAAGCCAACTGATGCAAATTGTTGTGTTGGTTACAAGTGTTCTAAATAACTTGAAACTCTTTCAGGACTACTGAATACTTTCTTGAATATACTTTCTTGTGTTGCACATTCTCTGTTCTAAAGAAATCATTCAATAATAGTTTCCAGGCTAAGAAATTTCATAGCTTTATGATCTCAACTTCATGGAATGTGCTTCCCAAGCTTACTAGATTGAGTCAACAATCTGATTATACCAGAAATGATTGTGTTTAATGTCACTCGTGCTTGATTGGTGGTTATAACTTCAAAAAAATTATCATTAATGTTTCAAAATTTTGTACAAACTTCATAGAGGCATTAAAAGACACTCCCTCATGTATGCCAAAAGGCCTAAAACTAAGGATTGGCAACAAAACAAAATCCAGAACTTGAATTAAATGACTTTAATACTTCTCAACTCTACTTGTCAAATTCCCAGATGACGCCTCCATCCTCTGCAAGTACACATCAAAAAGAAAAATCAATCACACTGTATACTGACACATATACTTTGAAAAGAGAAAAAAAAAAGAAGTCCTTAAGATATAGCTTCTAGATAAGGTTGATGATATAAGGTTAAATTACAAGTTTAGTCATAAAATTTCATGATTGTGTCAAATAGATCGACATTTTTCCAAATTCAGAGACTTTAACCTTTCACTTTGTGTCTAATAGGTCTATGAATATTAAAAAATGTCTAATAGGTCAATCAAGACTATTAAACACAAAATATAAAGTTCGATGGTGTTTTTAGACACTTAAGTTTGGAGACCTATTAGACAAGAAAACTAAAATCTT

mRNA sequence

CGAAAATTGATATCCGATATTAATTACTGAAAAGTTGAACTTCGTTCCTGAAAGTACTATTTCTCAGTGATGCACCGAACGGAAATGGGAAATTGAAACCATTAGGCAAATTAGCGAGCGGACAATTGGGAGATGGGATCGGCAGCAGCGCCATGGAAGTCCCTTCTTCTCACTGCTTTGGAATCTAATGGCCACATCAAGCATTCCCAATTCTTGCAGCTCGCAACGGTAGGAGCGAATGGAAGGCCCTCCAATCGCACAGTTGTTTTCAGAGGATTCCAAGAGGGCAGCGATAATATCCAAATCAACACGGATTCTAGAAGTCGCAAGGTTGAAGAGCTTAAGAATTGTCCATTTGCTGAGATATGCTGGTATTTCACCGAATCATGGGAGCAATTTCGGATCAGTGGGAGAGTTGAGGTTGTTGATTCATTGTATCCTGATGCTATAAAACTTCAGCAAAGAGAGAAAGCATGGTCCTCTATTCCTTCAAAGACAAGAATGCAGTATTTGGGACCTAATCCAGGCCTTCCCTATCTTACTGGACAACCAGCCAAGGAAAATCTTCAAGATTCTTCTTCGGGTCCTGTTGATTCATACTGTTTGCTTGTTTTCAATCCTGATCAGGTTGATTATGTGAATTTGAAGAGCGAAAAAAGGTTTTTGTATAAAGCGAGTTCGAGTTCAAGAGGGGAGAAGCTGTGGAGTACTGAGAGGATAAACCCTTAGCCACCGACAACGAGCTGCTATCCTAAAGCCAACTGATGCAAATTGTTGTGTTGGTTACAAGTGTTCTAAATAACTTGAAACTCTTTCAGGACTACTGAATACTTTCTTGAATATACTTTCTTGTGTTGCACATTCTCTGTTCTAAAGAAATCATTCAATAATAGTTTCCAGGCTAAGAAATTTCATAGCTTTATGATCTCAACTTCATGGAATGTGCTTCCCAAGCTTACTAGATTGAGTCAACAATCTGATTATACCAGAAATGATTGTGTTTAATGTCACTCGTGCTTGATTGGTGGTTATAACTTCAAAAAAATTATCATTAATGTTTCAAAATTTTGTACAAACTTCATAGAGGCATTAAAAGACACTCCCTCATGTATGCCAAAAGGCCTAAAACTAAGGATTGGCAACAAAACAAAATCCAGAACTTGAATTAAATGACTTTAATACTTCTCAACTCTACTTGTCAAATTCCCAGATGACGCCTCCATCCTCTGCAAGTACACATCAAAAAGAAAAATCAATCACACTGTATACTGACACATATACTTTGAAAAGAGAAAAAAAAAAGAAGTCCTTAAGATATAGCTTCTAGATAAGGTTGATGATATAAGGTTAAATTACAAGTTTAGTCATAAAATTTCATGATTGTGTCAAATAGATCGACATTTTTCCAAATTCAGAGACTTTAACCTTTCACTTTGTGTCTAATAGGTCTATGAATATTAAAAAATGTCTAATAGGTCAATCAAGACTATTAAACACAAAATATAAAGTTCGATGGTGTTTTTAGACACTTAAGTTTGGAGACCTATTAGACAAGAAAACTAAAATCTT

Coding sequence (CDS)

ATGGGATCGGCAGCAGCGCCATGGAAGTCCCTTCTTCTCACTGCTTTGGAATCTAATGGCCACATCAAGCATTCCCAATTCTTGCAGCTCGCAACGGTAGGAGCGAATGGAAGGCCCTCCAATCGCACAGTTGTTTTCAGAGGATTCCAAGAGGGCAGCGATAATATCCAAATCAACACGGATTCTAGAAGTCGCAAGGTTGAAGAGCTTAAGAATTGTCCATTTGCTGAGATATGCTGGTATTTCACCGAATCATGGGAGCAATTTCGGATCAGTGGGAGAGTTGAGGTTGTTGATTCATTGTATCCTGATGCTATAAAACTTCAGCAAAGAGAGAAAGCATGGTCCTCTATTCCTTCAAAGACAAGAATGCAGTATTTGGGACCTAATCCAGGCCTTCCCTATCTTACTGGACAACCAGCCAAGGAAAATCTTCAAGATTCTTCTTCGGGTCCTGTTGATTCATACTGTTTGCTTGTTTTCAATCCTGATCAGGTTGATTATGTGAATTTGAAGAGCGAAAAAAGGTTTTTGTATAAAGCGAGTTCGAGTTCAAGAGGGGAGAAGCTGTGGAGTACTGAGAGGATAAACCCTTAG

Protein sequence

MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINTDSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPSKTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYKASSSSRGEKLWSTERINP

Homology

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BLAST of MC05g0444 vs. ExPASy Swiss-Prot
Match: Q9ZPY1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PPOX2 PE=1 SV=2)

HSP 1 Score: 257.3 bits (656), Expect = 1.4e-67
Identity = 112/198 (56.57%), Postives = 154/198 (77.78%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MG+  APWK LL  A+E+N H+ HS ++QLAT+G NGRPSNRTVVFRGF+E SD IQINT
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           D RSRK+EELK+CPF+E+CWYF+++WEQFRI+GR+EV+D+  PD  KLQQREKAW +   
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           ++R+ Y+ P PG P  + Q +++   D SSGPV  YCLL+  P++VDY+NLK+ +R  + 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 181 ASSSSRGEKLWSTERINP 199
           + ++  GEK W++E++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

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BLAST of MC05g0444 vs. ExPASy Swiss-Prot
Match: P53210 (Uncharacterized protein YGR017W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YGR017W PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-07
Identity = 40/112 (35.71%), Postives = 55/112 (49.11%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVG-ANGRPSNRTVVFRGF---QEGSDNI 60
           M    APW  + + + ++N     S   Q ATV     +P  RTVVFR F    + ++ +
Sbjct: 1   MSHQMAPWIPMFIQSCKNNTEPFVS--FQFATVDELTNKPRCRTVVFRDFLFHDKRTNVL 60

Query: 61  QINTDSRSRKVEE------------LKNC--PFAEICWYFTESWEQFRISGR 95
             NTD RS K+ E             K C  PF E C+YF E+WEQ+R SG+
Sbjct: 61  TFNTDMRSSKITESFITPNSNNSSDSKRCETPFFEACFYFPETWEQYRFSGQ 110

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BLAST of MC05g0444 vs. ExPASy Swiss-Prot
Match: B1XYE2 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) OX=395495 GN=pdxH PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 4.8e-07
Identity = 36/98 (36.73%), Postives = 53/98 (54.08%), Query Frame = 0


Query: 28  LQLATVGANGRPSNRTVVFRGFQEGSDNIQINTDSRSRKVEELKNCPFAEICWYFTESWE 87
           + +ATV A+GRPS R V+ +G+   S  I   T+ +SRK +EL   PFA + +++     
Sbjct: 47  MTVATVSADGRPSTRVVLIKGYD--SRGIVWYTNYKSRKGQELAGNPFAALQFHWVTQER 106

Query: 88  QFRISGRVEVVDS----LYPDAIKLQQREKAWSSIPSK 122
             RI GRVE   +     Y D+  L  R  AW+S  S+
Sbjct: 107 VVRIEGRVEKTSAEESDAYYDSRPLDSRLGAWASPQSQ 142

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BLAST of MC05g0444 vs. ExPASy Swiss-Prot
Match: A2SJZ5 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Methylibium petroleiphilum (strain ATCC BAA-1232 / LMG 22953 / PM1) OX=420662 GN=pdxH PE=3 SV=2)

HSP 1 Score: 55.1 bits (131), Expect = 1.1e-06
Identity = 38/98 (38.78%), Postives = 51/98 (52.04%), Query Frame = 0


Query: 28  LQLATVGANGRPSNRTVVFRGFQEGSDNIQINTDSRSRKVEELKNCPFAEICWYFTESWE 87
           + LATVGA+GRPS R V+ +G    +  I   T+  SRK  EL   PFA + +++ +   
Sbjct: 48  MTLATVGADGRPSTRVVLIKGCD--ARGIVWYTNYESRKGRELAAHPFAALQFHWVDLER 107

Query: 88  QFRISGRVEVVDSLYPDAI----KLQQREKAWSSIPSK 122
             RI GRVE V     DA      L  R  AW+S  S+
Sbjct: 108 VVRIEGRVEKVSDTESDAYYRSRPLDSRLGAWASPQSQ 143

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BLAST of MC05g0444 vs. ExPASy Swiss-Prot
Match: A1TQY3 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Acidovorax citrulli (strain AAC00-1) OX=397945 GN=pdxH PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.4e-06
Identity = 38/98 (38.78%), Postives = 51/98 (52.04%), Query Frame = 0


Query: 28  LQLATVGANGRPSNRTVVFRGFQEGSDNIQINTDSRSRKVEELKNCPFAEICWYFTESWE 87
           + LATVGA+ RPS R V+ +G+ E    I   T+  SRK  +L   PFA + +++ E   
Sbjct: 60  MTLATVGADLRPSTRVVLIKGYDE--RGIVWYTNYGSRKGRQLAGNPFAALQFHWVELER 119

Query: 88  QFRISGRVEVVDSLYPDAI----KLQQREKAWSSIPSK 122
             RI GRVE V     DA      L  R  AW+S  S+
Sbjct: 120 VVRIEGRVEKVSDAESDAYFASRPLDSRIGAWASPQSE 155

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BLAST of MC05g0444 vs. NCBI nr
Match: XP_022147467.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Momordica charantia])

HSP 1 Score: 407 bits (1046), Expect = 1.99e-143
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT
Sbjct: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS
Sbjct: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK
Sbjct: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180

Query: 181 ASSSSRGEKLWSTERINP 198
           ASSSSRGEKLWSTERINP
Sbjct: 181 ASSSSRGEKLWSTERINP 198

[Close]

BLAST of MC05g0444 vs. NCBI nr
Match: XP_022147468.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X2 [Momordica charantia])

HSP 1 Score: 323 bits (827), Expect = 1.58e-110
Identity = 166/198 (83.84%), Postives = 166/198 (83.84%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT
Sbjct: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           DSRSRKVEELKNCPFAE                                QREKAWSSIPS
Sbjct: 61  DSRSRKVEELKNCPFAE--------------------------------QREKAWSSIPS 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK
Sbjct: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 166

Query: 181 ASSSSRGEKLWSTERINP 198
           ASSSSRGEKLWSTERINP
Sbjct: 181 ASSSSRGEKLWSTERINP 166

[Close]

BLAST of MC05g0444 vs. NCBI nr
Match: XP_038874699.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 319 bits (818), Expect = 1.14e-108
Identity = 154/198 (77.78%), Postives = 171/198 (86.36%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MGSAAAPWKSLLL ALESNG IKHS+FLQLAT+G NGRPSNRTVVFRGF+EG+D I I T
Sbjct: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           DSR+RK+EELKNCPFAEICWYFTESWEQFRISGR+EVVD+L  DA K +QR +AWSSI  
Sbjct: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           K+R+QYLGP+PGLPY   QP KE   DS SGPVDS+CLLVFNPDQVDY+NLKSE+R L+K
Sbjct: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180

Query: 181 ASSSSRGEKLWSTERINP 198
           A  S  GEKLW TERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]

BLAST of MC05g0444 vs. NCBI nr
Match: XP_038899249.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 314 bits (805), Expect = 1.09e-106
Identity = 152/198 (76.77%), Postives = 169/198 (85.35%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MGSAAAPWKSLLL ALESNG IKHS+FLQLAT+G NGRPSNRTVVFRGF+EG+D I I T
Sbjct: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDKIHIYT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           DSR+RK+EELKNCPFAEICWYFTESWEQFRISGR+EVVD+   DA K +QR +AWSSI  
Sbjct: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           K+R+QYLGP+PGLPY   QP KE   DS SGPVDS+CLLVFNPDQVDY+NLKSE+R L+K
Sbjct: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180

Query: 181 ASSSSRGEKLWSTERINP 198
           A  S  GEKLW T RINP
Sbjct: 181 AIPSFGGEKLWETGRINP 198

[Close]

BLAST of MC05g0444 vs. NCBI nr
Match: XP_004133803.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucumis sativus] >KGN56412.1 hypothetical protein Csa_010621 [Cucumis sativus])

HSP 1 Score: 312 bits (799), Expect = 8.92e-106
Identity = 147/197 (74.62%), Postives = 171/197 (86.80%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MGSA APWKSLLL+ALESNGH KHS+FLQLAT+G NGRPSNRTVVFRGF+EG+D I I T
Sbjct: 1   MGSAVAPWKSLLLSALESNGHFKHSKFLQLATIGTNGRPSNRTVVFRGFEEGTDRIHIYT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           DSR+RK+EELKNCPFAEICWYFTESWEQFRISGR+EVVD+   DA K +QR +AWSSI  
Sbjct: 61  DSRNRKIEELKNCPFAEICWYFTESWEQFRISGRMEVVDASCVDATKFKQRAEAWSSISP 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           K+RMQYLGP+PGLP+L  QPAKE   DS SGPVD++CLLVF+PDQVDY+N++S++R  +K
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSCSGPVDTFCLLVFDPDQVDYLNVRSDERNSFK 180

Query: 181 ASSSSRGEKLWSTERIN 197
           A +S  GEKLW TERIN
Sbjct: 181 AITSFGGEKLWETERIN 197

[Close]

BLAST of MC05g0444 vs. ExPASy TrEMBL
Match: A0A6J1D2F2 (Pyridoxal 5'-phosphate synthase OS=Momordica charantia OX=3673 GN=LOC111016386 PE=4 SV=1)

HSP 1 Score: 407 bits (1046), Expect = 9.66e-144
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT
Sbjct: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS
Sbjct: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK
Sbjct: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180

Query: 181 ASSSSRGEKLWSTERINP 198
           ASSSSRGEKLWSTERINP
Sbjct: 181 ASSSSRGEKLWSTERINP 198

[Close]

BLAST of MC05g0444 vs. ExPASy TrEMBL
Match: A0A6J1D1E2 (Pyridoxal 5'-phosphate synthase OS=Momordica charantia OX=3673 GN=LOC111016386 PE=4 SV=1)

HSP 1 Score: 323 bits (827), Expect = 7.67e-111
Identity = 166/198 (83.84%), Postives = 166/198 (83.84%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT
Sbjct: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           DSRSRKVEELKNCPFAE                                QREKAWSSIPS
Sbjct: 61  DSRSRKVEELKNCPFAE--------------------------------QREKAWSSIPS 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK
Sbjct: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 166

Query: 181 ASSSSRGEKLWSTERINP 198
           ASSSSRGEKLWSTERINP
Sbjct: 181 ASSSSRGEKLWSTERINP 166

[Close]

BLAST of MC05g0444 vs. ExPASy TrEMBL
Match: A0A0A0L745 (Pyridoxal 5'-phosphate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G119450 PE=4 SV=1)

HSP 1 Score: 312 bits (799), Expect = 4.32e-106
Identity = 147/197 (74.62%), Postives = 171/197 (86.80%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MGSA APWKSLLL+ALESNGH KHS+FLQLAT+G NGRPSNRTVVFRGF+EG+D I I T
Sbjct: 1   MGSAVAPWKSLLLSALESNGHFKHSKFLQLATIGTNGRPSNRTVVFRGFEEGTDRIHIYT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           DSR+RK+EELKNCPFAEICWYFTESWEQFRISGR+EVVD+   DA K +QR +AWSSI  
Sbjct: 61  DSRNRKIEELKNCPFAEICWYFTESWEQFRISGRMEVVDASCVDATKFKQRAEAWSSISP 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           K+RMQYLGP+PGLP+L  QPAKE   DS SGPVD++CLLVF+PDQVDY+N++S++R  +K
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSCSGPVDTFCLLVFDPDQVDYLNVRSDERNSFK 180

Query: 181 ASSSSRGEKLWSTERIN 197
           A +S  GEKLW TERIN
Sbjct: 181 AITSFGGEKLWETERIN 197

[Close]

BLAST of MC05g0444 vs. ExPASy TrEMBL
Match: A0A5A7U3K2 (Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00920 PE=4 SV=1)

HSP 1 Score: 311 bits (797), Expect = 8.71e-106
Identity = 149/198 (75.25%), Postives = 170/198 (85.86%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MGSA APWKSLLL ALESNG IKHS+FLQLAT+G NGRPSNRTVVFR F+EG+D I I T
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           DSRSRK+EELKNCPFAEICWYFT++WEQFRISGR+EVVD+   DA K +QR +AWSSI  
Sbjct: 61  DSRSRKIEELKNCPFAEICWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           K+RMQYLGP+PGLP+L  QPAKE   DS SGPVD++CLLVF+PDQVDY+NLKSE+R L++
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 ASSSSRGEKLWSTERINP 198
           A  S  GEKLW TERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]

BLAST of MC05g0444 vs. ExPASy TrEMBL
Match: A0A1S4DSQ7 (Pyridoxal 5'-phosphate synthase OS=Cucumis melo OX=3656 GN=LOC103483173 PE=4 SV=1)

HSP 1 Score: 311 bits (796), Expect = 1.24e-105
Identity = 148/198 (74.75%), Postives = 170/198 (85.86%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MGSA APWKSLLL ALESNG IKHS+FLQLAT+G NGRPSNRTVVFR F+EG+D I I T
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           DSRSRK+EELKNCPFAE+CWYFT++WEQFRISGR+EVVD+   DA K +QR +AWSSI  
Sbjct: 61  DSRSRKIEELKNCPFAEVCWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           K+RMQYLGP+PGLP+L  QPAKE   DS SGPVD++CLLVF+PDQVDY+NLKSE+R L++
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 ASSSSRGEKLWSTERINP 198
           A  S  GEKLW TERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]

BLAST of MC05g0444 vs. TAIR 10
Match: AT2G46580.1 (Pyridoxamine 5'-phosphate oxidase family protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.0e-68
Identity = 112/198 (56.57%), Postives = 154/198 (77.78%), Query Frame = 0


Query: 1   MGSAAAPWKSLLLTALESNGHIKHSQFLQLATVGANGRPSNRTVVFRGFQEGSDNIQINT 60
           MG+  APWK LL  A+E+N H+ HS ++QLAT+G NGRPSNRTVVFRGF+E SD IQINT
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 61  DSRSRKVEELKNCPFAEICWYFTESWEQFRISGRVEVVDSLYPDAIKLQQREKAWSSIPS 120
           D RSRK+EELK+CPF+E+CWYF+++WEQFRI+GR+EV+D+  PD  KLQQREKAW +   
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 121 KTRMQYLGPNPGLPYLTGQPAKENLQDSSSGPVDSYCLLVFNPDQVDYVNLKSEKRFLYK 180
           ++R+ Y+ P PG P  + Q +++   D SSGPV  YCLL+  P++VDY+NLK+ +R  + 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 181 ASSSSRGEKLWSTERINP 199
           + ++  GEK W++E++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

The following BLAST results are available for this feature:

BLAST of MC05g0444 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZPY1	1.4e-67	56.57	Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 G...	[more]
P53210	2.8e-07	35.71	Uncharacterized protein YGR017W OS=Saccharomyces cerevisiae (strain ATCC 204508 ...	[more]
B1XYE2	4.8e-07	36.73	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Leptothrix cholodnii (strain ATC...	[more]
A2SJZ5	1.1e-06	38.78	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Methylibium petroleiphilum (stra...	[more]
A1TQY3	1.4e-06	38.78	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Acidovorax citrulli (strain AAC0...	[more]

BLAST of MC05g0444 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147467.1	1.99e-143	100.00	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Momordica charantia]	[more]
XP_022147468.1	1.58e-110	83.84	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X2 [Momordica charantia]	[more]
XP_038874699.1	1.14e-108	77.78	pyridoxine/pyridoxamine 5'-phosphate oxidase 2-like isoform X2 [Benincasa hispid...	[more]
XP_038899249.1	1.09e-106	76.77	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Benincasa hispida]	[more]
XP_004133803.1	8.92e-106	74.62	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucumis sativus] >KGN...	[more]

BLAST of MC05g0444 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2F2	9.66e-144	100.00	Pyridoxal 5'-phosphate synthase OS=Momordica charantia OX=3673 GN=LOC111016386 P...	[more]
A0A6J1D1E2	7.67e-111	83.84	Pyridoxal 5'-phosphate synthase OS=Momordica charantia OX=3673 GN=LOC111016386 P...	[more]
A0A0A0L745	4.32e-106	74.62	Pyridoxal 5'-phosphate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G119450 PE=4 ...	[more]
A0A5A7U3K2	8.71e-106	75.25	Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_...	[more]
A0A1S4DSQ7	1.24e-105	74.75	Pyridoxal 5'-phosphate synthase OS=Cucumis melo OX=3656 GN=LOC103483173 PE=4 SV=...	[more]

BLAST of MC05g0444 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46580.1	1.0e-68	56.57	Pyridoxamine 5'-phosphate oxidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024624	Pyridoxamine 5'-phosphate oxidase, Alr4036 family, FMN-binding domain	PFAM	PF12766	Pyridox_oxase_2	coord: 6..98 e-value: 1.0E-29 score: 103.0
IPR012349	FMN-binding split barrel	GENE3D	2.30.110.10		coord: 3..198 e-value: 2.4E-57 score: 195.9
IPR024015	Pyridoxamine 5'-phosphate oxidase, probable FMN-dependent, Alr4036 family	TIGRFAM	TIGR04026	TIGR04026	coord: 6..198 e-value: 1.4E-49 score: 166.5
IPR000659	Pyridoxamine 5'-phosphate oxidase	PANTHER	PTHR10851	PYRIDOXINE-5-PHOSPHATE OXIDASE	coord: 2..198
None	No IPR available	PANTHER	PTHR10851:SF3	PYRIDOXINE/PYRIDOXAMINE 5'-PHOSPHATE OXIDASE 2	coord: 2..198
None	No IPR available	SUPERFAMILY	50475	FMN-binding split barrel	coord: 4..198

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0444.1	MC05g0444.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042823	pyridoxal phosphate biosynthetic process
biological_process	GO:0008615	pyridoxine biosynthetic process
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0004733	pyridoxamine-phosphate oxidase activity