MC05g0436 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0436 vs. ExPASy Swiss-Prot
Match: Q9SSE9 (Lysine-specific demethylase JMJ25 OS=Arabidopsis thaliana OX=3702 GN=JMJ25 PE=1 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 1.6e-166
Identity = 337/804 (41.92%), Postives = 450/804 (55.97%), Query Frame = 0

Query: 192 MCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCRGHCNCKACLRE 251
           MCHQCQ++D+  V  C  C+ KR+C+ C++ WYP   +EDV   C FC   CNC+ACLR 
Sbjct: 154 MCHQCQKSDRI-VERCQTCNSKRYCHPCLDTWYPLIAKEDVAKKCMFCSSTCNCRACLR- 213

Query: 252 FVEVVPKELDASV------KVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGAQLTEV 311
            ++   K +++++      KV+  KF+L  +LP L+ I  EQ +E E+E  I G +  EV
Sbjct: 214 -LDTKLKGINSNLIVSEEEKVQASKFILQSLLPHLKGINDEQVAEKEVEAKIYGLKFEEV 273

Query: 312 DVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRES----------- 371
             +  K    ER+YCD C TSI++ +R+C   +CSFD+CLSCC E+R             
Sbjct: 274 RPQDAKAFPDERLYCDICKTSIYDLHRNC--KSCSFDICLSCCLEIRNGKALACKEDVSW 333

Query: 372 -------FHPKGSEINICRTIFRNKLSSNSIMSDYL-----------LAGRECQLTSTYK 431
                   +  G E  +      NKL     + D L             GR         
Sbjct: 334 NYINRGLEYEHGQEGKVIEKP-ANKLDDK--LKDKLDGKPDDKPKGKPKGRPKGKPDDKP 393

Query: 432 NGMSSSFQG----------------------WSANPDGSIPCPPKQSGGCGIANLELRRT 491
            G     Q                       W AN  G I C       CG   L L+R 
Sbjct: 394 KGKLKGKQDDKPDDKPDEKPVNTDHMKYPSLWKANEAGIITC------CCGAGELVLKRL 453

Query: 492 FKADWTTKLI-------EGAEGLTSDYKVPDSC-SSEVCPSCYLNSNEVRQAAFRENSHD 551
               W ++L+       E  E L     V + C  S       ++S  + +AA RE S D
Sbjct: 454 LPDGWISELVNRVEKTAEAGELLNLPETVLERCPCSNSDRHIDIDSCNLLKAACREGSED 513

Query: 552 NFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQTGANVK 611
           N+LYSP+  D+  D + HFQ HW+KGEPVIVRNVL+ TSGLSWEPMVM RA RQ  ++V+
Sbjct: 514 NYLYSPSVWDVQQDDLKHFQHHWVKGEPVIVRNVLEATSGLSWEPMVMHRACRQI-SHVQ 573

Query: 612 FKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTSFEERLP 671
                + V A+DC D CEV++N+H+FF GY +GR  R GWP +LKLKDWP +  F++ LP
Sbjct: 574 -HGSLKDVVAVDCLDFCEVKVNLHEFFTGYTDGRYDRMGWPLVLKLKDWPPAKVFKDNLP 633

Query: 672 RHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSRGDSVTK 731
           RH  EF+ +LP   YTHP  G LNLA KLP+  LKPDMGPKTY+A GF +EL RGDSVTK
Sbjct: 634 RHAEEFLCSLPLKHYTHPVNGPLNLAVKLPQNCLKPDMGPKTYVASGFAQELGRGDSVTK 693

Query: 732 LHCDMSDAVNVLTHTSKV-NIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASKKEILG 791
           LHCDMSDAVN+LTH S+V N++P     I   +K+ A +DL +LY  +    A+K+E++ 
Sbjct: 694 LHCDMSDAVNILTHISEVPNMQPG----IGNLKKKHAEQDLKELYSSV----ANKEEMM- 753

Query: 792 CTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAM 851
                                                    I++ +   ++N E  +   
Sbjct: 754 ----------------------------------------EILENSRQQVQNVETDD--- 813

Query: 852 GGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEE 911
            GA+WDIFRR+D+PK+  Y+EKH KEFRH+   PV+ +VHPIHDQ  +    H  +LKEE
Sbjct: 814 -GALWDIFRREDIPKLESYIEKHHKEFRHLYCCPVSQVVHPIHDQNFYLTRYHIMKLKEE 873

Query: 912 FGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEFRLLPK 930
           +G+EPWTF Q +G+AV IP GCPHQVRN +SC KVA+DFVSPENV EC +LT+++RLLP 
Sbjct: 874 YGIEPWTFNQKLGDAVLIPVGCPHQVRNLKSCNKVALDFVSPENVSECLRLTKQYRLLPP 888

BLAST of MC05g0436 vs. ExPASy Swiss-Prot
Match: Q9Y4C1 (Lysine-specific demethylase 3A OS=Homo sapiens OX=9606 GN=KDM3A PE=1 SV=4)

HSP 1 Score: 201.1 bits (510), Expect = 5.7e-50
Identity = 139/427 (32.55%), Postives = 204/427 (47.78%), Query Frame = 0

Query: 505  FQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQTGANVKFKEETRRVKAIDC-FDLC 564
            F+  W +G+PV+V  V  K +   W+P     +FR+     +F E+   V  ++C  +  
Sbjct: 966  FRECWKQGQPVMVSGVHHKLNSELWKP----ESFRK-----EFGEQ--EVDLVNCRTNEI 1025

Query: 565  EVEINIHQFFVGY--LEGRMYRNGWPEMLKLKDWPSSTSFEERLPRHCAEFIAALPYSEY 624
                 +  F+ G+  +  R+     P +LKLKDWP    F + +P    + +A +P  EY
Sbjct: 1026 ITGATVGDFWDGFEDVPNRLKNEKEPMVLKLKDWPPGEDFRDMMPSRFDDLMANIPLPEY 1085

Query: 625  THPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSRGDSVTKLHCDMSDAVNVLTHT 684
            T  + G LNLA++LP   ++PD+GPK Y AYG      R    T LH D+SDA NV+ + 
Sbjct: 1086 TR-RDGKLNLASRLPNYFVRPDLGPKMYNAYGLITPEDRKYGTTNLHLDVSDAANVMVYV 1145

Query: 685  SKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASKKEILGCTSSQQCGQSSDDTTK 744
                        I K Q +   E L  +                     Q G S + T K
Sbjct: 1146 G-----------IPKGQCEQEEEVLKTI---------------------QDGDSDELTIK 1205

Query: 745  IFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAMGGAVWDIFRRQDVPKI 804
             F                               +E  EK      GA+W I+  +D  KI
Sbjct: 1206 RF-------------------------------IEGKEK-----PGALWHIYAAKDTEKI 1265

Query: 805  AKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEEFGVEPWTFEQFIGEAV 864
             ++L+K  +E    +  P +    PIHDQ+ + +   +K+L +E+GV+ W   QF+G+ V
Sbjct: 1266 REFLKKVSEE--QGQENPADH--DPIHDQSWYLDRSLRKRLHQEYGVQGWAIVQFLGDVV 1308

Query: 865  FIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEFRLLPKNHRAKEDKLELKKMTL 924
            FIPAG PHQV N  SCIKVA DFVSPE+V+ CF LT+EFR L + H   EDKL++K +  
Sbjct: 1326 FIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFWLTQEFRYLSQTHTNHEDKLQVKNVIY 1308

Query: 925  YAASSAI 929
            +A   A+
Sbjct: 1386 HAVKDAV 1308

BLAST of MC05g0436 vs. ExPASy Swiss-Prot
Match: Q7LBC6 (Lysine-specific demethylase 3B OS=Homo sapiens OX=9606 GN=KDM3B PE=1 SV=2)

HSP 1 Score: 200.3 bits (508), Expect = 9.6e-50
Identity = 139/429 (32.40%), Postives = 203/429 (47.32%), Query Frame = 0

Query: 505  FQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQTGANVKFKEETRRVKAIDCFDLCE 564
            F+  W +G+PV+V  V  K     W+P    + F             + V  ++C +   
Sbjct: 1405 FRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGD-----------QDVDLVNCRNCAI 1464

Query: 565  V-EINIHQFFVGY---LEGRMYRNGWPEMLKLKDWPSSTSFEERLPRHCAEFIAALPYSE 624
            + ++ +  F+ G+    +     +G P +LKLKDWP    F + +P    + +  LP  E
Sbjct: 1465 ISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPE 1524

Query: 625  YTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSRGDSVTKLHCDMSDAVNVLTH 684
            YT  + G LNLA++LP   ++PD+GPK Y AYG      R    T LH D+SDAVNV+  
Sbjct: 1525 YT-KRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVM-- 1584

Query: 685  TSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGID-GLKASKKEILGCTSSQQCGQSSDDT 744
                                        +Y GI  G  A  +E+L               
Sbjct: 1585 ----------------------------VYVGIPIGEGAHDEEVL--------------- 1644

Query: 745  TKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAMGGAVWDIFRRQDVP 804
             K  +E            D +  +K  I D  +              GA+W I+  +D  
Sbjct: 1645 -KTIDEG-----------DADEVTKQRIHDGKEK------------PGALWHIYAAKDAE 1704

Query: 805  KIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEEFGVEPWTFEQFIGE 864
            KI + L K  +E +  +N P +    PIHDQ+ + +   +K+L EE+GV+ W   QF+G+
Sbjct: 1705 KIRELLRKVGEE-QGQENPPDHD---PIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGD 1748

Query: 865  AVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEFRLLPKNHRAKEDKLELKKM 924
            AVFIPAG PHQV N  SCIKVA DFVSPE+V+ CF+LT+EFR L   H   EDKL++K +
Sbjct: 1765 AVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNI 1748

Query: 925  TLYAASSAI 929
              +A   A+
Sbjct: 1825 IYHAVKDAV 1748

BLAST of MC05g0436 vs. ExPASy Swiss-Prot
Match: Q5ZIX8 (Lysine-specific demethylase 3A OS=Gallus gallus OX=9031 GN=KDM3A PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 6.2e-49
Identity = 136/428 (31.78%), Postives = 201/428 (46.96%), Query Frame = 0

Query: 505  FQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQTGANVKFKEETRRVKAIDC-FDLC 564
            F+  W +G+PV+V  V  K +   W P    + F Q           + V  ++C  +  
Sbjct: 969  FRECWKQGQPVMVSGVHHKLNADLWRPESFRKEFGQ-----------QEVDLVNCRTNEI 1028

Query: 565  EVEINIHQFFVGY--LEGRM-YRNGWPEMLKLKDWPSSTSFEERLPRHCAEFIAALPYSE 624
                 +  F+ G+  +  R+    G P +LKLKDWP    F + +P    + +  +P  E
Sbjct: 1029 ITGATVGDFWDGFEDISSRLRTEEGEPMVLKLKDWPPGEDFRDMMPSRFDDLMKNIPLPE 1088

Query: 625  YTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSRGDSVTKLHCDMSDAVNVLTH 684
            YT  + G LNLA++LP   ++PD+GPK Y AYG      R    T LH D+SDA NV+  
Sbjct: 1089 YTR-RGGKLNLASRLPNYFVRPDLGPKMYNAYGLITPEDRKYGTTNLHLDVSDAANVM-- 1148

Query: 685  TSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASKKEILGCTSSQQCGQSSDDTT 744
                                        +Y GI   +A ++E +    + Q G S + T 
Sbjct: 1149 ----------------------------VYVGIPKGQADQEEEV--LKTIQDGDSDELTI 1208

Query: 745  KIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAMGGAVWDIFRRQDVPK 804
            K F E+ +                                      GA+W I+  +D  K
Sbjct: 1209 KRFTESREKP------------------------------------GALWHIYAAKDTEK 1268

Query: 805  IAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEEFGVEPWTFEQFIGEA 864
            I ++L+K  +E    +  PV+    PIHDQ+ + +   +K+L +E+GV+ W   QF+G+ 
Sbjct: 1269 IREFLKKVAEE--QGQENPVDH--DPIHDQSWYLDRSLRKRLHQEYGVQGWAIVQFLGDV 1312

Query: 865  VFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEFRLLPKNHRAKEDKLELKKMT 924
            VFIPAG PHQV N  SCIKVA DFVSPE+V+ CF LT+EFR L   H   EDKL++K + 
Sbjct: 1329 VFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFWLTQEFRYLSHTHTNHEDKLQVKNVI 1312

Query: 925  LYAASSAI 929
             +A   A+
Sbjct: 1389 YHAVKDAV 1312

BLAST of MC05g0436 vs. ExPASy Swiss-Prot
Match: Q6ZPY7 (Lysine-specific demethylase 3B OS=Mus musculus OX=10090 GN=Kdm3b PE=1 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 1.1e-48
Identity = 134/428 (31.31%), Postives = 200/428 (46.73%), Query Frame = 0

Query: 505  FQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQTGANVKFKEETRRVKAIDCFDLCE 564
            F+  W +G+PV+V  V  K     W+P    + F             + V  ++C +   
Sbjct: 1206 FRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGD-----------QDVDLVNCRNCAI 1265

Query: 565  V-EINIHQFFVGY---LEGRMYRNGWPEMLKLKDWPSSTSFEERLPRHCAEFIAALPYSE 624
            + ++ +  F+ G+    +     +G P +LKLKDWP    F + +P    + +  LP  E
Sbjct: 1266 ISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPE 1325

Query: 625  YTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSRGDSVTKLHCDMSDAVNVLTH 684
            YT  + G LNLA++LP   ++PD+GPK Y AYG      R    T LH D+SDAVNV+ +
Sbjct: 1326 YT-KRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVY 1385

Query: 685  TSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASKKEILGCTSSQQCGQSSDDTT 744
                                              G+   +            G   ++  
Sbjct: 1386 V---------------------------------GIPVGE------------GAHDEEVL 1445

Query: 745  KIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAMGGAVWDIFRRQDVPK 804
            K  +E            D +  +K  I D  +              GA+W I+  +D  K
Sbjct: 1446 KTIDEG-----------DADEVTKQRIHDGKEK------------PGALWHIYAAKDAEK 1505

Query: 805  IAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEEFGVEPWTFEQFIGEA 864
            I + L K  +E +  +N P +    PIHDQ+ + +   +K+L EE+GV+ W   QF+G+A
Sbjct: 1506 IRELLRKVGEE-QGQENPPDHD---PIHDQSWYLDQILRKRLFEEYGVQGWAIVQFLGDA 1549

Query: 865  VFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEFRLLPKNHRAKEDKLELKKMT 924
            VFIPAG PHQV N  SCIKVA DFVSPE+V+ CF+LT+EFR L   H   EDKL++K + 
Sbjct: 1566 VFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNII 1549

Query: 925  LYAASSAI 929
             +A   A+
Sbjct: 1626 YHAVKDAV 1549

BLAST of MC05g0436 vs. NCBI nr
Match: XP_022147261.1 (lysine-specific demethylase JMJ25 isoform X1 [Momordica charantia])

HSP 1 Score: 1851 bits (4795), Expect = 0.0
Identity = 901/929 (96.99%), Postives = 901/929 (96.99%), Query Frame = 0

Query: 1   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 60
           DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG
Sbjct: 7   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 66

Query: 61  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 120
           GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE
Sbjct: 67  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 126

Query: 121 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHKRNHVLANGKLVEV 180
           FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQ KSAKHKRNHVLANGKLVEV
Sbjct: 127 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQSKSAKHKRNHVLANGKLVEV 186

Query: 181 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 240
           GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR
Sbjct: 187 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 246

Query: 241 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 300
           GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA
Sbjct: 247 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 306

Query: 301 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKGS 360
           QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG 
Sbjct: 307 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG- 366

Query: 361 EINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 420
                                     RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ
Sbjct: 367 --------------------------RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 426

Query: 421 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 480
           SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF
Sbjct: 427 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 486

Query: 481 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 540
           RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ
Sbjct: 487 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 546

Query: 541 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 600
           TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS
Sbjct: 547 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 606

Query: 601 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 660
           FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR
Sbjct: 607 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 666

Query: 661 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 720
           GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK
Sbjct: 667 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 726

Query: 721 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 780
           KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK
Sbjct: 727 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 786

Query: 781 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 840
           MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK
Sbjct: 787 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 846

Query: 841 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF 900
           QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF
Sbjct: 847 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF 906

Query: 901 RLLPKNHRAKEDKLELKKMTLYAASSAIR 929
           RLLPKNHRAKEDKLELKKMTLYAASSAIR
Sbjct: 907 RLLPKNHRAKEDKLELKKMTLYAASSAIR 908

BLAST of MC05g0436 vs. NCBI nr
Match: XP_022147262.1 (lysine-specific demethylase JMJ25 isoform X2 [Momordica charantia])

HSP 1 Score: 1845 bits (4778), Expect = 0.0
Identity = 900/929 (96.88%), Postives = 900/929 (96.88%), Query Frame = 0

Query: 1   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 60
           DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG
Sbjct: 7   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 66

Query: 61  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 120
           GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE
Sbjct: 67  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 126

Query: 121 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHKRNHVLANGKLVEV 180
           FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQ KSAKHKRNHVLANGKLVE 
Sbjct: 127 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQSKSAKHKRNHVLANGKLVE- 186

Query: 181 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 240
           GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR
Sbjct: 187 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 246

Query: 241 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 300
           GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA
Sbjct: 247 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 306

Query: 301 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKGS 360
           QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG 
Sbjct: 307 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG- 366

Query: 361 EINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 420
                                     RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ
Sbjct: 367 --------------------------RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 426

Query: 421 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 480
           SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF
Sbjct: 427 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 486

Query: 481 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 540
           RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ
Sbjct: 487 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 546

Query: 541 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 600
           TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS
Sbjct: 547 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 606

Query: 601 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 660
           FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR
Sbjct: 607 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 666

Query: 661 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 720
           GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK
Sbjct: 667 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 726

Query: 721 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 780
           KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK
Sbjct: 727 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 786

Query: 781 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 840
           MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK
Sbjct: 787 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 846

Query: 841 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF 900
           QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF
Sbjct: 847 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF 906

Query: 901 RLLPKNHRAKEDKLELKKMTLYAASSAIR 929
           RLLPKNHRAKEDKLELKKMTLYAASSAIR
Sbjct: 907 RLLPKNHRAKEDKLELKKMTLYAASSAIR 907

BLAST of MC05g0436 vs. NCBI nr
Match: XP_022147263.1 (lysine-specific demethylase JMJ25 isoform X3 [Momordica charantia])

HSP 1 Score: 1753 bits (4540), Expect = 0.0
Identity = 861/929 (92.68%), Postives = 861/929 (92.68%), Query Frame = 0

Query: 1   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 60
           DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG
Sbjct: 7   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 66

Query: 61  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 120
           GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE
Sbjct: 67  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 126

Query: 121 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHKRNHVLANGKLVEV 180
           FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQ KSAKHKRNHVLANGKLVEV
Sbjct: 127 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQSKSAKHKRNHVLANGKLVEV 186

Query: 181 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 240
           GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR
Sbjct: 187 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 246

Query: 241 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 300
           GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA
Sbjct: 247 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 306

Query: 301 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKGS 360
           QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG 
Sbjct: 307 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG- 366

Query: 361 EINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 420
                                     RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ
Sbjct: 367 --------------------------RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 426

Query: 421 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 480
           SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF
Sbjct: 427 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 486

Query: 481 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 540
           RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ
Sbjct: 487 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 546

Query: 541 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 600
           TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS
Sbjct: 547 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 606

Query: 601 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 660
           FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR
Sbjct: 607 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 666

Query: 661 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 720
           GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK
Sbjct: 667 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 726

Query: 721 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 780
           KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK
Sbjct: 727 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 786

Query: 781 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 840
           MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK
Sbjct: 787 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 846

Query: 841 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF 900
           QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQ                         
Sbjct: 847 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQ------------------------- 868

Query: 901 RLLPKNHRAKEDKLELKKMTLYAASSAIR 929
                          LKKMTLYAASSAIR
Sbjct: 907 ---------------LKKMTLYAASSAIR 868

BLAST of MC05g0436 vs. NCBI nr
Match: XP_022980026.1 (lysine-specific demethylase JMJ25 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1438 bits (3722), Expect = 0.0
Identity = 738/1032 (71.51%), Postives = 792/1032 (76.74%), Query Frame = 0

Query: 1    DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKG-CCSGEATAA 60
            DS  ALRCK +DGARWRC+E ASPGKSYCE+HLIQLMK+SL+RVR  G G CCSG+    
Sbjct: 15   DSPDALRCKCSDGARWRCKELASPGKSYCERHLIQLMKRSLNRVRNSGDGGCCSGDG--- 74

Query: 61   GGSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGE 120
                           + +  RKEV+    GS    +EDEL RNG LVRK  R+LS     
Sbjct: 75   ---------------RVSANRKEVRGVRFGSQGKETEDELERNGRLVRKKNRKLSNIH-- 134

Query: 121  EFPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHKRNHVLANGKLVE 180
                KDVT   DSGK +F++ +LSHGKN      A+SAERQG S K KRNHV+ANGK VE
Sbjct: 135  ---FKDVTISSDSGKPQFTASKLSHGKNG-----ADSAERQGTSTKRKRNHVVANGKSVE 194

Query: 181  VGTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFC 240
               PNKK+GGSLMCHQCQ++D+SGVV C  CHRKRFCY+CIERWYPDRTREDVENACP C
Sbjct: 195  TAMPNKKNGGSLMCHQCQKSDRSGVVICSNCHRKRFCYQCIERWYPDRTREDVENACPCC 254

Query: 241  RGHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQG 300
            RGHCNCKACLREFVE  PKELDASVKVERLKFLLYKVLP+LRHIQREQS ELE+E NI+G
Sbjct: 255  RGHCNCKACLREFVEFAPKELDASVKVERLKFLLYKVLPILRHIQREQSYELEVECNIKG 314

Query: 301  AQLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG 360
            A+L EVD++RIKLD TERMYCD+CNTSIFNFYRSCFNPNCS+DLCLSCCKELRESFH +G
Sbjct: 315  ARLKEVDIKRIKLDQTERMYCDSCNTSIFNFYRSCFNPNCSYDLCLSCCKELRESFHSEG 374

Query: 361  SEINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKN---GMSSSFQGWSANPDGSIPC 420
            S+INI   +FRNKLSS  +M   +LAGRE QLTST K    G SSSFQ WSANPDGSIPC
Sbjct: 375  SKINIYFELFRNKLSSQ-LMCINVLAGREYQLTSTSKTSVGGTSSSFQVWSANPDGSIPC 434

Query: 421  PPKQSGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVR 480
            PPKQ GGCGIANLELRR+ KADW TKLIEGAEG TSDYKVPDSCSSEVC SC  N NEVR
Sbjct: 435  PPKQCGGCGIANLELRRSLKADWATKLIEGAEGFTSDYKVPDSCSSEVCSSCCFNKNEVR 494

Query: 481  QAAFRENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCR 540
             AAFRENSHDNFLYSPNA D+MDD V+HFQ HWMKGEPVIVRNVLDKTSGLSWEPMVM R
Sbjct: 495  PAAFRENSHDNFLYSPNADDVMDDGVNHFQRHWMKGEPVIVRNVLDKTSGLSWEPMVMWR 554

Query: 541  AFRQTGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWP 600
            AFRQTGAN KFKEET  VKAIDCFD CEV INIHQFFVGYLEGRM+RNGWPEMLKLKDWP
Sbjct: 555  AFRQTGANAKFKEETCSVKAIDCFDWCEVVINIHQFFVGYLEGRMHRNGWPEMLKLKDWP 614

Query: 601  SSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQE 660
            SSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYG QE
Sbjct: 615  SSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGLQE 674

Query: 661  ELSRGDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGI--- 720
            ELSRGDSVTKLHCDMSDAVNVLTHT+KVNIK WQ   IEKRQK+FAAED S+LYGGI   
Sbjct: 675  ELSRGDSVTKLHCDMSDAVNVLTHTNKVNIKRWQRDFIEKRQKRFAAEDRSELYGGIKST 734

Query: 721  ------------------------------------------------------------ 780
                                                                        
Sbjct: 735  FDDTKKDSECEQNKVADQKSCLAGLFATIRKRVTKPVKCANKDPLLIQKPRGESKSQSRK 794

Query: 781  ------------------------------------DGLKASKKEILGCTSSQQCGQSSD 840
                                                +G K + K  + CT SQQCGQSS+
Sbjct: 795  QFDEHEYSSSKLTSVTIRNSSVGTFSTGALANIFCPNGPKTAHKVAIACTPSQQCGQSSN 854

Query: 841  DTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAMGGAVWDIFRRQD 900
            DT+K FNE CDSEKASG NE N+  SK SI D ADSHLE +EKMEVA GGAVWDIFRRQD
Sbjct: 855  DTSKFFNEKCDSEKASGRNEVNDPTSKDSISDGADSHLEKNEKMEVATGGAVWDIFRRQD 914

Query: 901  VPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEEFGVEPWTFEQFI 929
            VPK+ KYL+KHQKEFRHMK KPVNSLVHPIHDQT+FFNAKHKKQLKEEFGVEPWTFEQFI
Sbjct: 915  VPKLLKYLDKHQKEFRHMKCKPVNSLVHPIHDQTVFFNAKHKKQLKEEFGVEPWTFEQFI 974

BLAST of MC05g0436 vs. NCBI nr
Match: XP_022980025.1 (lysine-specific demethylase JMJ25 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1433 bits (3709), Expect = 0.0
Identity = 738/1034 (71.37%), Postives = 792/1034 (76.60%), Query Frame = 0

Query: 1    DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKG-CCSGEATAA 60
            DS  ALRCK +DGARWRC+E ASPGKSYCE+HLIQLMK+SL+RVR  G G CCSG+    
Sbjct: 15   DSPDALRCKCSDGARWRCKELASPGKSYCERHLIQLMKRSLNRVRNSGDGGCCSGDG--- 74

Query: 61   GGSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGE 120
                           + +  RKEV+    GS    +EDEL RNG LVRK  R+LS     
Sbjct: 75   ---------------RVSANRKEVRGVRFGSQGKETEDELERNGRLVRKKNRKLSNIH-- 134

Query: 121  EFPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHKRNHVLANGKLVE 180
                KDVT   DSGK +F++ +LSHGKN      A+SAERQG S K KRNHV+ANGK VE
Sbjct: 135  ---FKDVTISSDSGKPQFTASKLSHGKNG-----ADSAERQGTSTKRKRNHVVANGKSVE 194

Query: 181  V--GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACP 240
                 PNKK+GGSLMCHQCQ++D+SGVV C  CHRKRFCY+CIERWYPDRTREDVENACP
Sbjct: 195  TVQAMPNKKNGGSLMCHQCQKSDRSGVVICSNCHRKRFCYQCIERWYPDRTREDVENACP 254

Query: 241  FCRGHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNI 300
             CRGHCNCKACLREFVE  PKELDASVKVERLKFLLYKVLP+LRHIQREQS ELE+E NI
Sbjct: 255  CCRGHCNCKACLREFVEFAPKELDASVKVERLKFLLYKVLPILRHIQREQSYELEVECNI 314

Query: 301  QGAQLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHP 360
            +GA+L EVD++RIKLD TERMYCD+CNTSIFNFYRSCFNPNCS+DLCLSCCKELRESFH 
Sbjct: 315  KGARLKEVDIKRIKLDQTERMYCDSCNTSIFNFYRSCFNPNCSYDLCLSCCKELRESFHS 374

Query: 361  KGSEINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKN---GMSSSFQGWSANPDGSI 420
            +GS+INI   +FRNKLSS  +M   +LAGRE QLTST K    G SSSFQ WSANPDGSI
Sbjct: 375  EGSKINIYFELFRNKLSSQ-LMCINVLAGREYQLTSTSKTSVGGTSSSFQVWSANPDGSI 434

Query: 421  PCPPKQSGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNE 480
            PCPPKQ GGCGIANLELRR+ KADW TKLIEGAEG TSDYKVPDSCSSEVC SC  N NE
Sbjct: 435  PCPPKQCGGCGIANLELRRSLKADWATKLIEGAEGFTSDYKVPDSCSSEVCSSCCFNKNE 494

Query: 481  VRQAAFRENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVM 540
            VR AAFRENSHDNFLYSPNA D+MDD V+HFQ HWMKGEPVIVRNVLDKTSGLSWEPMVM
Sbjct: 495  VRPAAFRENSHDNFLYSPNADDVMDDGVNHFQRHWMKGEPVIVRNVLDKTSGLSWEPMVM 554

Query: 541  CRAFRQTGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKD 600
             RAFRQTGAN KFKEET  VKAIDCFD CEV INIHQFFVGYLEGRM+RNGWPEMLKLKD
Sbjct: 555  WRAFRQTGANAKFKEETCSVKAIDCFDWCEVVINIHQFFVGYLEGRMHRNGWPEMLKLKD 614

Query: 601  WPSSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGF 660
            WPSSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYG 
Sbjct: 615  WPSSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGL 674

Query: 661  QEELSRGDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGI- 720
            QEELSRGDSVTKLHCDMSDAVNVLTHT+KVNIK WQ   IEKRQK+FAAED S+LYGGI 
Sbjct: 675  QEELSRGDSVTKLHCDMSDAVNVLTHTNKVNIKRWQRDFIEKRQKRFAAEDRSELYGGIK 734

Query: 721  ------------------------------------------------------------ 780
                                                                        
Sbjct: 735  STFDDTKKDSECEQNKVADQKSCLAGLFATIRKRVTKPVKCANKDPLLIQKPRGESKSQS 794

Query: 781  --------------------------------------DGLKASKKEILGCTSSQQCGQS 840
                                                  +G K + K  + CT SQQCGQS
Sbjct: 795  RKQFDEHEYSSSKLTSVTIRNSSVGTFSTGALANIFCPNGPKTAHKVAIACTPSQQCGQS 854

Query: 841  SDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAMGGAVWDIFRR 900
            S+DT+K FNE CDSEKASG NE N+  SK SI D ADSHLE +EKMEVA GGAVWDIFRR
Sbjct: 855  SNDTSKFFNEKCDSEKASGRNEVNDPTSKDSISDGADSHLEKNEKMEVATGGAVWDIFRR 914

Query: 901  QDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEEFGVEPWTFEQ 929
            QDVPK+ KYL+KHQKEFRHMK KPVNSLVHPIHDQT+FFNAKHKKQLKEEFGVEPWTFEQ
Sbjct: 915  QDVPKLLKYLDKHQKEFRHMKCKPVNSLVHPIHDQTVFFNAKHKKQLKEEFGVEPWTFEQ 974

BLAST of MC05g0436 vs. ExPASy TrEMBL
Match: A0A6J1D1U1 (lysine-specific demethylase JMJ25 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016257 PE=4 SV=1)

HSP 1 Score: 1851 bits (4795), Expect = 0.0
Identity = 901/929 (96.99%), Postives = 901/929 (96.99%), Query Frame = 0

Query: 1   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 60
           DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG
Sbjct: 7   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 66

Query: 61  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 120
           GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE
Sbjct: 67  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 126

Query: 121 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHKRNHVLANGKLVEV 180
           FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQ KSAKHKRNHVLANGKLVEV
Sbjct: 127 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQSKSAKHKRNHVLANGKLVEV 186

Query: 181 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 240
           GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR
Sbjct: 187 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 246

Query: 241 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 300
           GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA
Sbjct: 247 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 306

Query: 301 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKGS 360
           QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG 
Sbjct: 307 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG- 366

Query: 361 EINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 420
                                     RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ
Sbjct: 367 --------------------------RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 426

Query: 421 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 480
           SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF
Sbjct: 427 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 486

Query: 481 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 540
           RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ
Sbjct: 487 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 546

Query: 541 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 600
           TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS
Sbjct: 547 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 606

Query: 601 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 660
           FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR
Sbjct: 607 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 666

Query: 661 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 720
           GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK
Sbjct: 667 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 726

Query: 721 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 780
           KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK
Sbjct: 727 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 786

Query: 781 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 840
           MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK
Sbjct: 787 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 846

Query: 841 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF 900
           QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF
Sbjct: 847 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF 906

Query: 901 RLLPKNHRAKEDKLELKKMTLYAASSAIR 929
           RLLPKNHRAKEDKLELKKMTLYAASSAIR
Sbjct: 907 RLLPKNHRAKEDKLELKKMTLYAASSAIR 908

BLAST of MC05g0436 vs. ExPASy TrEMBL
Match: A0A6J1D1W5 (lysine-specific demethylase JMJ25 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016257 PE=4 SV=1)

HSP 1 Score: 1845 bits (4778), Expect = 0.0
Identity = 900/929 (96.88%), Postives = 900/929 (96.88%), Query Frame = 0

Query: 1   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 60
           DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG
Sbjct: 7   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 66

Query: 61  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 120
           GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE
Sbjct: 67  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 126

Query: 121 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHKRNHVLANGKLVEV 180
           FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQ KSAKHKRNHVLANGKLVE 
Sbjct: 127 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQSKSAKHKRNHVLANGKLVE- 186

Query: 181 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 240
           GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR
Sbjct: 187 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 246

Query: 241 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 300
           GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA
Sbjct: 247 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 306

Query: 301 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKGS 360
           QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG 
Sbjct: 307 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG- 366

Query: 361 EINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 420
                                     RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ
Sbjct: 367 --------------------------RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 426

Query: 421 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 480
           SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF
Sbjct: 427 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 486

Query: 481 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 540
           RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ
Sbjct: 487 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 546

Query: 541 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 600
           TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS
Sbjct: 547 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 606

Query: 601 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 660
           FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR
Sbjct: 607 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 666

Query: 661 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 720
           GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK
Sbjct: 667 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 726

Query: 721 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 780
           KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK
Sbjct: 727 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 786

Query: 781 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 840
           MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK
Sbjct: 787 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 846

Query: 841 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF 900
           QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF
Sbjct: 847 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF 906

Query: 901 RLLPKNHRAKEDKLELKKMTLYAASSAIR 929
           RLLPKNHRAKEDKLELKKMTLYAASSAIR
Sbjct: 907 RLLPKNHRAKEDKLELKKMTLYAASSAIR 907

BLAST of MC05g0436 vs. ExPASy TrEMBL
Match: A0A6J1D0U1 (lysine-specific demethylase JMJ25 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016257 PE=4 SV=1)

HSP 1 Score: 1753 bits (4540), Expect = 0.0
Identity = 861/929 (92.68%), Postives = 861/929 (92.68%), Query Frame = 0

Query: 1   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 60
           DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG
Sbjct: 7   DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKGCCSGEATAAG 66

Query: 61  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 120
           GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE
Sbjct: 67  GSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGEE 126

Query: 121 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHKRNHVLANGKLVEV 180
           FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQ KSAKHKRNHVLANGKLVEV
Sbjct: 127 FPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQSKSAKHKRNHVLANGKLVEV 186

Query: 181 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 240
           GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR
Sbjct: 187 GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 246

Query: 241 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 300
           GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA
Sbjct: 247 GHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGA 306

Query: 301 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKGS 360
           QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG 
Sbjct: 307 QLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG- 366

Query: 361 EINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 420
                                     RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ
Sbjct: 367 --------------------------RECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQ 426

Query: 421 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 480
           SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF
Sbjct: 427 SGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAF 486

Query: 481 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 540
           RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ
Sbjct: 487 RENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQ 546

Query: 541 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 600
           TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS
Sbjct: 547 TGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTS 606

Query: 601 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 660
           FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR
Sbjct: 607 FEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSR 666

Query: 661 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 720
           GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK
Sbjct: 667 GDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASK 726

Query: 721 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 780
           KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK
Sbjct: 727 KEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEK 786

Query: 781 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 840
           MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK
Sbjct: 787 MEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKK 846

Query: 841 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEF 900
           QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQ                         
Sbjct: 847 QLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQ------------------------- 868

Query: 901 RLLPKNHRAKEDKLELKKMTLYAASSAIR 929
                          LKKMTLYAASSAIR
Sbjct: 907 ---------------LKKMTLYAASSAIR 868

BLAST of MC05g0436 vs. ExPASy TrEMBL
Match: A0A6J1ISE8 (lysine-specific demethylase JMJ25 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479552 PE=4 SV=1)

HSP 1 Score: 1438 bits (3722), Expect = 0.0
Identity = 738/1032 (71.51%), Postives = 792/1032 (76.74%), Query Frame = 0

Query: 1    DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKG-CCSGEATAA 60
            DS  ALRCK +DGARWRC+E ASPGKSYCE+HLIQLMK+SL+RVR  G G CCSG+    
Sbjct: 15   DSPDALRCKCSDGARWRCKELASPGKSYCERHLIQLMKRSLNRVRNSGDGGCCSGDG--- 74

Query: 61   GGSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGE 120
                           + +  RKEV+    GS    +EDEL RNG LVRK  R+LS     
Sbjct: 75   ---------------RVSANRKEVRGVRFGSQGKETEDELERNGRLVRKKNRKLSNIH-- 134

Query: 121  EFPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHKRNHVLANGKLVE 180
                KDVT   DSGK +F++ +LSHGKN      A+SAERQG S K KRNHV+ANGK VE
Sbjct: 135  ---FKDVTISSDSGKPQFTASKLSHGKNG-----ADSAERQGTSTKRKRNHVVANGKSVE 194

Query: 181  VGTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFC 240
               PNKK+GGSLMCHQCQ++D+SGVV C  CHRKRFCY+CIERWYPDRTREDVENACP C
Sbjct: 195  TAMPNKKNGGSLMCHQCQKSDRSGVVICSNCHRKRFCYQCIERWYPDRTREDVENACPCC 254

Query: 241  RGHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQG 300
            RGHCNCKACLREFVE  PKELDASVKVERLKFLLYKVLP+LRHIQREQS ELE+E NI+G
Sbjct: 255  RGHCNCKACLREFVEFAPKELDASVKVERLKFLLYKVLPILRHIQREQSYELEVECNIKG 314

Query: 301  AQLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG 360
            A+L EVD++RIKLD TERMYCD+CNTSIFNFYRSCFNPNCS+DLCLSCCKELRESFH +G
Sbjct: 315  ARLKEVDIKRIKLDQTERMYCDSCNTSIFNFYRSCFNPNCSYDLCLSCCKELRESFHSEG 374

Query: 361  SEINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKN---GMSSSFQGWSANPDGSIPC 420
            S+INI   +FRNKLSS  +M   +LAGRE QLTST K    G SSSFQ WSANPDGSIPC
Sbjct: 375  SKINIYFELFRNKLSSQ-LMCINVLAGREYQLTSTSKTSVGGTSSSFQVWSANPDGSIPC 434

Query: 421  PPKQSGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVR 480
            PPKQ GGCGIANLELRR+ KADW TKLIEGAEG TSDYKVPDSCSSEVC SC  N NEVR
Sbjct: 435  PPKQCGGCGIANLELRRSLKADWATKLIEGAEGFTSDYKVPDSCSSEVCSSCCFNKNEVR 494

Query: 481  QAAFRENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCR 540
             AAFRENSHDNFLYSPNA D+MDD V+HFQ HWMKGEPVIVRNVLDKTSGLSWEPMVM R
Sbjct: 495  PAAFRENSHDNFLYSPNADDVMDDGVNHFQRHWMKGEPVIVRNVLDKTSGLSWEPMVMWR 554

Query: 541  AFRQTGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWP 600
            AFRQTGAN KFKEET  VKAIDCFD CEV INIHQFFVGYLEGRM+RNGWPEMLKLKDWP
Sbjct: 555  AFRQTGANAKFKEETCSVKAIDCFDWCEVVINIHQFFVGYLEGRMHRNGWPEMLKLKDWP 614

Query: 601  SSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQE 660
            SSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYG QE
Sbjct: 615  SSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGLQE 674

Query: 661  ELSRGDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGI--- 720
            ELSRGDSVTKLHCDMSDAVNVLTHT+KVNIK WQ   IEKRQK+FAAED S+LYGGI   
Sbjct: 675  ELSRGDSVTKLHCDMSDAVNVLTHTNKVNIKRWQRDFIEKRQKRFAAEDRSELYGGIKST 734

Query: 721  ------------------------------------------------------------ 780
                                                                        
Sbjct: 735  FDDTKKDSECEQNKVADQKSCLAGLFATIRKRVTKPVKCANKDPLLIQKPRGESKSQSRK 794

Query: 781  ------------------------------------DGLKASKKEILGCTSSQQCGQSSD 840
                                                +G K + K  + CT SQQCGQSS+
Sbjct: 795  QFDEHEYSSSKLTSVTIRNSSVGTFSTGALANIFCPNGPKTAHKVAIACTPSQQCGQSSN 854

Query: 841  DTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAMGGAVWDIFRRQD 900
            DT+K FNE CDSEKASG NE N+  SK SI D ADSHLE +EKMEVA GGAVWDIFRRQD
Sbjct: 855  DTSKFFNEKCDSEKASGRNEVNDPTSKDSISDGADSHLEKNEKMEVATGGAVWDIFRRQD 914

Query: 901  VPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEEFGVEPWTFEQFI 929
            VPK+ KYL+KHQKEFRHMK KPVNSLVHPIHDQT+FFNAKHKKQLKEEFGVEPWTFEQFI
Sbjct: 915  VPKLLKYLDKHQKEFRHMKCKPVNSLVHPIHDQTVFFNAKHKKQLKEEFGVEPWTFEQFI 974

BLAST of MC05g0436 vs. ExPASy TrEMBL
Match: A0A6J1IV00 (lysine-specific demethylase JMJ25 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479552 PE=4 SV=1)

HSP 1 Score: 1433 bits (3709), Expect = 0.0
Identity = 738/1034 (71.37%), Postives = 792/1034 (76.60%), Query Frame = 0

Query: 1    DSSGALRCKRNDGARWRCREFASPGKSYCEKHLIQLMKQSLHRVRKGGKG-CCSGEATAA 60
            DS  ALRCK +DGARWRC+E ASPGKSYCE+HLIQLMK+SL+RVR  G G CCSG+    
Sbjct: 15   DSPDALRCKCSDGARWRCKELASPGKSYCERHLIQLMKRSLNRVRNSGDGGCCSGDG--- 74

Query: 61   GGSSHRDVHASVRVMKETTTRKEVKDGGCGSHEVASEDELGRNGILVRKHKRQLSTYDGE 120
                           + +  RKEV+    GS    +EDEL RNG LVRK  R+LS     
Sbjct: 75   ---------------RVSANRKEVRGVRFGSQGKETEDELERNGRLVRKKNRKLSNIH-- 134

Query: 121  EFPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHKRNHVLANGKLVE 180
                KDVT   DSGK +F++ +LSHGKN      A+SAERQG S K KRNHV+ANGK VE
Sbjct: 135  ---FKDVTISSDSGKPQFTASKLSHGKNG-----ADSAERQGTSTKRKRNHVVANGKSVE 194

Query: 181  V--GTPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACP 240
                 PNKK+GGSLMCHQCQ++D+SGVV C  CHRKRFCY+CIERWYPDRTREDVENACP
Sbjct: 195  TVQAMPNKKNGGSLMCHQCQKSDRSGVVICSNCHRKRFCYQCIERWYPDRTREDVENACP 254

Query: 241  FCRGHCNCKACLREFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNI 300
             CRGHCNCKACLREFVE  PKELDASVKVERLKFLLYKVLP+LRHIQREQS ELE+E NI
Sbjct: 255  CCRGHCNCKACLREFVEFAPKELDASVKVERLKFLLYKVLPILRHIQREQSYELEVECNI 314

Query: 301  QGAQLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHP 360
            +GA+L EVD++RIKLD TERMYCD+CNTSIFNFYRSCFNPNCS+DLCLSCCKELRESFH 
Sbjct: 315  KGARLKEVDIKRIKLDQTERMYCDSCNTSIFNFYRSCFNPNCSYDLCLSCCKELRESFHS 374

Query: 361  KGSEINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKN---GMSSSFQGWSANPDGSI 420
            +GS+INI   +FRNKLSS  +M   +LAGRE QLTST K    G SSSFQ WSANPDGSI
Sbjct: 375  EGSKINIYFELFRNKLSSQ-LMCINVLAGREYQLTSTSKTSVGGTSSSFQVWSANPDGSI 434

Query: 421  PCPPKQSGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNE 480
            PCPPKQ GGCGIANLELRR+ KADW TKLIEGAEG TSDYKVPDSCSSEVC SC  N NE
Sbjct: 435  PCPPKQCGGCGIANLELRRSLKADWATKLIEGAEGFTSDYKVPDSCSSEVCSSCCFNKNE 494

Query: 481  VRQAAFRENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVM 540
            VR AAFRENSHDNFLYSPNA D+MDD V+HFQ HWMKGEPVIVRNVLDKTSGLSWEPMVM
Sbjct: 495  VRPAAFRENSHDNFLYSPNADDVMDDGVNHFQRHWMKGEPVIVRNVLDKTSGLSWEPMVM 554

Query: 541  CRAFRQTGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKD 600
             RAFRQTGAN KFKEET  VKAIDCFD CEV INIHQFFVGYLEGRM+RNGWPEMLKLKD
Sbjct: 555  WRAFRQTGANAKFKEETCSVKAIDCFDWCEVVINIHQFFVGYLEGRMHRNGWPEMLKLKD 614

Query: 601  WPSSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGF 660
            WPSSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYG 
Sbjct: 615  WPSSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGL 674

Query: 661  QEELSRGDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGI- 720
            QEELSRGDSVTKLHCDMSDAVNVLTHT+KVNIK WQ   IEKRQK+FAAED S+LYGGI 
Sbjct: 675  QEELSRGDSVTKLHCDMSDAVNVLTHTNKVNIKRWQRDFIEKRQKRFAAEDRSELYGGIK 734

Query: 721  ------------------------------------------------------------ 780
                                                                        
Sbjct: 735  STFDDTKKDSECEQNKVADQKSCLAGLFATIRKRVTKPVKCANKDPLLIQKPRGESKSQS 794

Query: 781  --------------------------------------DGLKASKKEILGCTSSQQCGQS 840
                                                  +G K + K  + CT SQQCGQS
Sbjct: 795  RKQFDEHEYSSSKLTSVTIRNSSVGTFSTGALANIFCPNGPKTAHKVAIACTPSQQCGQS 854

Query: 841  SDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAMGGAVWDIFRR 900
            S+DT+K FNE CDSEKASG NE N+  SK SI D ADSHLE +EKMEVA GGAVWDIFRR
Sbjct: 855  SNDTSKFFNEKCDSEKASGRNEVNDPTSKDSISDGADSHLEKNEKMEVATGGAVWDIFRR 914

Query: 901  QDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEEFGVEPWTFEQ 929
            QDVPK+ KYL+KHQKEFRHMK KPVNSLVHPIHDQT+FFNAKHKKQLKEEFGVEPWTFEQ
Sbjct: 915  QDVPKLLKYLDKHQKEFRHMKCKPVNSLVHPIHDQTVFFNAKHKKQLKEEFGVEPWTFEQ 974

BLAST of MC05g0436 vs. TAIR 10
Match: AT4G00990.1 (Transcription factor jumonji (jmjC) domain-containing protein )

HSP 1 Score: 812.0 bits (2096), Expect = 5.0e-235
Identity = 438/839 (52.21%), Postives = 562/839 (66.98%), Query Frame = 0

Query: 130 RDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKHK-RNHVLANGKLVEVGTPNK--K 189
           RDSG+        S  K    ++K N    +G+  + + R  V      +++  P K  K
Sbjct: 12  RDSGELVEDGRSESERKT---RKKENDVVSKGRIGRGRGRGEVSKRSIEIDISNPEKDIK 71

Query: 190 DGGS-----LMCHQCQ-RNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCR 249
             GS       CH C+    +S ++FC KC++K +C+ CI+R Y +RT E+V  ACPFC 
Sbjct: 72  PDGSRKCLGSTCHHCKILTSESDLIFCSKCNKKCYCFDCIKRSYSERTHEEVRAACPFCM 131

Query: 250 GHCNCKACLR-EFVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQG 309
             C C+ACLR   V   P E D  VK+++L++LL KVLPVL+ I  EQ+ ELEIE  I+G
Sbjct: 132 MTCICRACLRLPLVIKPPSEKDTDVKLKQLQYLLVKVLPVLKDIYTEQNRELEIESTIRG 191

Query: 310 AQLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKG 369
             +TE +++R KLD +ER+YCD C TSI NF+RSC N NCS D+CLSCCKEL E FH + 
Sbjct: 192 HPVTEANIKRCKLDPSERIYCDLCRTSIANFHRSCPNKNCSVDICLSCCKELSEGFHQER 251

Query: 370 SEINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKNGMSS----SFQGWSANPDGSIP 429
                       K ++          G EC++ +       +     F  W  N D SIP
Sbjct: 252 D----------GKKNAEG-------KGYECRIPAGQGKDSDAYVPLHFSTWKLNSDSSIP 311

Query: 430 CPPKQSGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEV 489
           CPPK+ GGCG + LELRR +K DW  KLI  AE  T +++  D      C SC  NS+ +
Sbjct: 312 CPPKECGGCGTSTLELRRLWKRDWVEKLITNAEKCTLNFRPTDVDIVHECSSCSTNSDSI 371

Query: 490 -RQAAFRENSHDNFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVM 549
            RQAAFR+N+HDNFLYSPNA D+ +D + HFQFHWMK EPVIVRNVL+KTSGLSWEPMVM
Sbjct: 372 RRQAAFRKNAHDNFLYSPNAVDLAEDDIAHFQFHWMKAEPVIVRNVLEKTSGLSWEPMVM 431

Query: 550 CRAFRQTGANVK-FKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLK 609
            RA R+     K  +EET +VKA+DC D CEVEIN+HQFF GYLEGRM++NGWPEMLKLK
Sbjct: 432 WRACREMDPKRKGTEEETTKVKALDCLDWCEVEINLHQFFEGYLEGRMHKNGWPEMLKLK 491

Query: 610 DWPSSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYG 669
           DWP S  FE+RLPRH AEFIAALP+ +YT PK G+LNLAT+ PEGSLKPD+GPKTYIAYG
Sbjct: 492 DWPPSDLFEKRLPRHNAEFIAALPFFDYTDPKSGILNLATRFPEGSLKPDLGPKTYIAYG 551

Query: 670 FQEELSRGDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDL-SQLYGG 729
           F EEL+RGDSVTKLHCD+SDAVNVLTHT+KV I P + + I+  QK++A   L  Q Y G
Sbjct: 552 FHEELNRGDSVTKLHCDISDAVNVLTHTAKVEIPPVKYQNIKVHQKKYAEAMLQKQQYSG 611

Query: 730 ------------IDGLKASKKEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNN 789
                       +  +  SKK++    ++++  QS++ +    + + ++EK     EDN 
Sbjct: 612 QVKEASELENKSMKEVDESKKDLKDKAANEE--QSNNSSRP--SGSGEAEKVIISKEDNP 671

Query: 790 LR-----SKGSIMD-RADSHLEND----EKMEVAMGGAVWDIFRRQDVPKIAKYLEKHQK 849
            +     S  SI + + D+  E D    E+ +   GGAVWDIFRR+DVPK+ ++L++H+ 
Sbjct: 672 TQPAVSTSVESIQEQKLDAPKETDGNTNERSKAVHGGAVWDIFRREDVPKLIQFLKRHEH 731

Query: 850 EFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEEFGVEPWTFEQFIGEAVFIPAGCPHQ 909
           EFRH  N+P+ S++HPIHDQT+F +   KKQLKEEF +EPWTFEQ +GEAVFIPAGCPHQ
Sbjct: 732 EFRHFNNEPLESVIHPIHDQTMFLSDSQKKQLKEEFDIEPWTFEQHLGEAVFIPAGCPHQ 791

Query: 910 VRNRQSCIKVAMDFVSPENVEECFQLTEEFRLLPKNHRAKEDKLELKKMTLYAASSAIR 930
           VRNRQSCIKVA+DFV+PE+VEEC +LT+EFR LPK+H + EDKLELKK+ LYAASSAIR
Sbjct: 792 VRNRQSCIKVALDFVAPESVEECLRLTQEFRRLPKDHSSSEDKLELKKIALYAASSAIR 826

BLAST of MC05g0436 vs. TAIR 10
Match: AT1G11950.1 (Transcription factor jumonji (jmjC) domain-containing protein )

HSP 1 Score: 631.3 bits (1627), Expect = 1.2e-180
Identity = 327/745 (43.89%), Postives = 450/745 (60.40%), Query Frame = 0

Query: 188 GGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCRGHCNCKA 247
           G   +CHQC + ++  +  C  C  + +C+ CI++WYP  + +D+   CPFCRG CNC  
Sbjct: 188 GELAICHQCSKGERRYLFICTFCEVRLYCFPCIKKWYPHLSTDDILEKCPFCRGTCNCCT 247

Query: 248 CLRE--FVEVVPKELDASVKVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGAQLTEV 307
           CL     +E   ++LD   +   L+FL+  +LP L+ + + Q  E+E E  +Q +  ++V
Sbjct: 248 CLHSSGLIETSKRKLDKYERFYHLRFLIVAMLPFLKKLCKAQDQEIETEAKVQDSMASQV 307

Query: 308 DVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRESFHPKGSEINIC 367
           D+      N ER++C++C TSI + +RSC  P CS++LCL+CC+E+R  +     E   C
Sbjct: 308 DISESLCSNEERVFCNHCATSIVDLHRSC--PKCSYELCLNCCQEIRGGWLSDRPE---C 367

Query: 368 RTIFRNKLSSNSIMSDYLLAGRECQLTSTYKNGMSSSFQGWSANPDGSIPCPPKQSGGCG 427
           +  F  +  +  I  +      E   +S  ++   +    W+A+ +GSI C PK+ GGCG
Sbjct: 368 QLQFEYR-GTRYIHGE----AAEPSSSSVSEDETKTPSIKWNADENGSIRCAPKELGGCG 427

Query: 428 IANLELRRTFKADWTTKLIEGAEGLTSDYKVPDSCSSEVCPSCYLNSNEVRQAAFRENSH 487
            + LEL+R     W + L + AE   + Y +    S   C S    S+  R+AA R+ S 
Sbjct: 428 DSVLELKRILPVTWMSDLEQKAETFLASYSIKPPMSYCRCSSDM--SSMKRKAASRDGSS 487

Query: 488 DNFLYSPNATDIM-DDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQTGAN 547
           DN+LYSP++ D++  + + HFQ HW KGEPVIVRN L+ T+GLSWEPMVM RA  +   +
Sbjct: 488 DNYLYSPDSLDVLKQEELLHFQEHWSKGEPVIVRNALNNTAGLSWEPMVMWRALCE-NVD 547

Query: 548 VKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTSFEER 607
                    VKAIDC   CEV+IN   FF GY +GR Y N WPEMLKLKDWP S  FE  
Sbjct: 548 SAISSNMSDVKAIDCLANCEVKINTLCFFEGYSKGRTYENFWPEMLKLKDWPPSDKFENL 607

Query: 608 LPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSRGDSV 667
           LPRHC EFI+ALP+ EY+ P+ G+LN+ATKLPEG LKPD+GPKTY+AYG  +EL RGDSV
Sbjct: 608 LPRHCDEFISALPFQEYSDPRSGILNIATKLPEGLLKPDLGPKTYVAYGTSDELGRGDSV 667

Query: 668 TKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASKKEIL 727
           TKLHCDMSDAVN+L HT++V +           +++ A  DL Q +      + ++KE+ 
Sbjct: 668 TKLHCDMSDAVNILMHTAEVTL---------SEEQRSAIADLKQKH-----KQQNEKEL- 727

Query: 728 GCTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVA 787
                                           E N L  +  + D    + E        
Sbjct: 728 -------------------------------QEQNGLEEEEVVSDEIVVYDET------- 787

Query: 788 MGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKE 847
             GA+WDIF+R+DVPK+ +YL KH  EFRH     V  + HPIHDQ+ F   +HK++LK 
Sbjct: 788 -SGALWDIFKREDVPKLEEYLRKHCIEFRHTYCSRVTKVYHPIHDQSYFLTVEHKRKLKA 847

Query: 848 EFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEFRLLP 907
           EFG+EPWTF Q +GEAVFIPAGCPHQVRN +SC KVA+DFVSPEN++EC +LT+EFR LP
Sbjct: 848 EFGIEPWTFVQKLGEAVFIPAGCPHQVRNLKSCTKVAVDFVSPENIDECLRLTDEFRQLP 865

Query: 908 KNHRAKEDKLELKKMTLYAASSAIR 930
           KNH+A+EDKLE+KKM +YA   A++
Sbjct: 908 KNHKAREDKLEIKKMVIYAVEQALK 865

BLAST of MC05g0436 vs. TAIR 10
Match: AT1G62310.1 (transcription factor jumonji (jmjC) domain-containing protein )

HSP 1 Score: 631.3 bits (1627), Expect = 1.2e-180
Identity = 355/823 (43.13%), Postives = 478/823 (58.08%), Query Frame = 0

Query: 107 RKHKRQLSTYDGEEFPCKDVTSVRDSGKSEFSSFELSHGKNNFRKEKANSAERQGKSAKH 166
           RK KRQ+   + EE+  ++VT          S  + +  ++      ANS E        
Sbjct: 129 RKDKRQVEVDEDEEWE-EEVTLC--------SKIKATSSRSRTHSLSANSPENVTDVISP 188

Query: 167 KRNHVLANGKLVEVG----TPNKKDGGSLMCHQCQRNDKSGVVFCLKCHRKRFCYKCIER 226
            R+   A+     +     T ++K  G + CHQC + ++  ++ C +C +  FC +CI +
Sbjct: 189 CRSRSPASNVSDSIQKNDCTSSRKQSGPI-CHQCLKGERITLLICSECEKTMFCLQCIRK 248

Query: 227 WYPDRTREDVENACPFCRGHCNCKAC--LREFVEVVPKELDASVKVERLKFLLYKVLPVL 286
           WYP+ + +DV   CP CR +CNC  C  L   +E   +EL  S +   L++L+  +LP L
Sbjct: 249 WYPNLSEDDVVEKCPLCRQNCNCSKCLHLNGLIETSKRELAKSERRHHLQYLITLMLPFL 308

Query: 287 RHIQREQSSELEIEGNIQGAQLTEVDVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCS 346
             +   Q  E+E E  +QG   +EV++        ER+YCD+C TSI + +RSC  P CS
Sbjct: 309 NKLSIFQKLEIEFEATVQGKLPSEVEITAAISYTDERVYCDHCATSIVDLHRSC--PKCS 368

Query: 347 FDLCLSCCKELRESFHPKGSEINICRTIFRNKLSSNSIMSDYLLAGRECQLTSTYKNGMS 406
           ++LCL CC+E+RE    +  E+      F      +  M  + L   E  L+ST+++  +
Sbjct: 369 YELCLKCCQEIREGSLSERPEMK-----FHYVDRGHRYM--HGLDAAEPSLSSTFEDEEA 428

Query: 407 S-SFQGWSANPDGSIPCPPKQSGGCGIANLELRRTFKADWTTKLIEGAEGLTSDYKVPDS 466
           + S   WS   +GSI C P++ GGCG   LELRR     W + L   AE   S Y +   
Sbjct: 429 NPSDAKWSLGENGSITCAPEKLGGCGERMLELRRILPLTWMSDLEHKAETFLSSYNISPR 488

Query: 467 CSSEVCPSCYLNSNEVRQAAFRENSHDNFLYSPNATDIM-DDAVDHFQFHWMKGEPVIVR 526
             +  C S  L +   R++A R  S DN+L+ P +  ++ ++ + HFQ HW KGEPVIVR
Sbjct: 489 MLNCRCSS--LETELTRKSASRTTSSDNYLFCPESLGVLKEEELLHFQEHWAKGEPVIVR 548

Query: 527 NVLDKTSGLSWEPMVMCRAFRQTGANVKFKEETRRVKAIDCFDLCEVEINIHQFFVGYLE 586
           N LD T GLSWEPMVM RA  +   N     E  +VKAIDC   CEVEIN  QFF GY +
Sbjct: 549 NALDNTPGLSWEPMVMWRALCE-NVNSTSSSEMSQVKAIDCLANCEVEINTRQFFEGYSK 608

Query: 587 GRMYRNGWPEMLKLKDWPSSTSFEERLPRHCAEFIAALPYSEYTHPKYGLLNLATKLPEG 646
           GR Y N WPEMLKLKDWP S  FE+ LPRHC EFI+ALP+ EY+ P+ G+LN+ATKLPEG
Sbjct: 609 GRTYENFWPEMLKLKDWPPSDKFEDLLPRHCDEFISALPFQEYSDPRTGILNIATKLPEG 668

Query: 647 SLKPDMGPKTYIAYGFQEELSRGDSVTKLHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQ 706
            +KPD+GPKTYIAYG  +EL RGDSVTKLHCDMSDAVN+LTHT++V +   Q        
Sbjct: 669 FIKPDLGPKTYIAYGIPDELGRGDSVTKLHCDMSDAVNILTHTAEVTLSQEQ-------- 728

Query: 707 KQFAAEDLSQLYGGIDGLKASKKEILGCTSSQQCGQSSDDTTKIFNENCDSEKASGCNED 766
                         I  +KA K++                  K+ N+  D +    CNE 
Sbjct: 729 --------------ISSVKALKQK-----------------HKLQNK-VDKQSTEDCNEK 788

Query: 767 NNLRSKGSIMDRADSHLENDEKMEVAMGGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNK 826
                +   M    S+ EN+E      G A+WDIFRR+DVPK+ +YL KH KEFRH    
Sbjct: 789 EEEEEEELNMPEISSN-ENEE-----TGSALWDIFRREDVPKLEEYLRKHCKEFRHTYCS 848

Query: 827 PVNSLVHPIHDQTIFFNAKHKKQLKEEFGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCI 886
           PV  + HPIHDQ+ +   +HK++LK E+G+EPWTF Q +GEAVFIPAGCPHQVRN +SC 
Sbjct: 849 PVTKVYHPIHDQSCYLTLEHKRKLKAEYGIEPWTFVQKLGEAVFIPAGCPHQVRNLKSCT 883

Query: 887 KVAMDFVSPENVEECFQLTEEFRLLPKNHRAKEDKLELKKMTL 922
           KVA+DFVSPEN+ EC +LTEEFR LPKNH+A+EDKLE   ++L
Sbjct: 909 KVAVDFVSPENIHECLRLTEEFRQLPKNHKAREDKLEASLLSL 883

BLAST of MC05g0436 vs. TAIR 10
Match: AT3G07610.1 (Transcription factor jumonji (jmjC) domain-containing protein )

HSP 1 Score: 588.2 bits (1515), Expect = 1.2e-167
Identity = 337/804 (41.92%), Postives = 450/804 (55.97%), Query Frame = 0

Query: 192 MCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCRGHCNCKACLRE 251
           MCHQCQ++D+  V  C  C+ KR+C+ C++ WYP   +EDV   C FC   CNC+ACLR 
Sbjct: 154 MCHQCQKSDRI-VERCQTCNSKRYCHPCLDTWYPLIAKEDVAKKCMFCSSTCNCRACLR- 213

Query: 252 FVEVVPKELDASV------KVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGAQLTEV 311
            ++   K +++++      KV+  KF+L  +LP L+ I  EQ +E E+E  I G +  EV
Sbjct: 214 -LDTKLKGINSNLIVSEEEKVQASKFILQSLLPHLKGINDEQVAEKEVEAKIYGLKFEEV 273

Query: 312 DVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRES----------- 371
             +  K    ER+YCD C TSI++ +R+C   +CSFD+CLSCC E+R             
Sbjct: 274 RPQDAKAFPDERLYCDICKTSIYDLHRNC--KSCSFDICLSCCLEIRNGKALACKEDVSW 333

Query: 372 -------FHPKGSEINICRTIFRNKLSSNSIMSDYL-----------LAGRECQLTSTYK 431
                   +  G E  +      NKL     + D L             GR         
Sbjct: 334 NYINRGLEYEHGQEGKVIEKP-ANKLDDK--LKDKLDGKPDDKPKGKPKGRPKGKPDDKP 393

Query: 432 NGMSSSFQG----------------------WSANPDGSIPCPPKQSGGCGIANLELRRT 491
            G     Q                       W AN  G I C       CG   L L+R 
Sbjct: 394 KGKLKGKQDDKPDDKPDEKPVNTDHMKYPSLWKANEAGIITC------CCGAGELVLKRL 453

Query: 492 FKADWTTKLI-------EGAEGLTSDYKVPDSC-SSEVCPSCYLNSNEVRQAAFRENSHD 551
               W ++L+       E  E L     V + C  S       ++S  + +AA RE S D
Sbjct: 454 LPDGWISELVNRVEKTAEAGELLNLPETVLERCPCSNSDRHIDIDSCNLLKAACREGSED 513

Query: 552 NFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQTGANVK 611
           N+LYSP+  D+  D + HFQ HW+KGEPVIVRNVL+ TSGLSWEPMVM RA RQ  ++V+
Sbjct: 514 NYLYSPSVWDVQQDDLKHFQHHWVKGEPVIVRNVLEATSGLSWEPMVMHRACRQI-SHVQ 573

Query: 612 FKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTSFEERLP 671
                + V A+DC D CEV++N+H+FF GY +GR  R GWP +LKLKDWP +  F++ LP
Sbjct: 574 -HGSLKDVVAVDCLDFCEVKVNLHEFFTGYTDGRYDRMGWPLVLKLKDWPPAKVFKDNLP 633

Query: 672 RHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSRGDSVTK 731
           RH  EF+ +LP   YTHP  G LNLA KLP+  LKPDMGPKTY+A GF +EL RGDSVTK
Sbjct: 634 RHAEEFLCSLPLKHYTHPVNGPLNLAVKLPQNCLKPDMGPKTYVASGFAQELGRGDSVTK 693

Query: 732 LHCDMSDAVNVLTHTSKV-NIKPWQTKIIEKRQKQFAAEDLSQLYGGIDGLKASKKEILG 791
           LHCDMSDAVN+LTH S+V N++P     I   +K+ A +DL +LY  +    A+K+E++ 
Sbjct: 694 LHCDMSDAVNILTHISEVPNMQPG----IGNLKKKHAEQDLKELYSSV----ANKEEMM- 753

Query: 792 CTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAM 851
                                                    I++ +   ++N E  +   
Sbjct: 754 ----------------------------------------EILENSRQQVQNVETDD--- 813

Query: 852 GGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEE 911
            GA+WDIFRR+D+PK+  Y+EKH KEFRH+   PV+ +VHPIHDQ  +    H  +LKEE
Sbjct: 814 -GALWDIFRREDIPKLESYIEKHHKEFRHLYCCPVSQVVHPIHDQNFYLTRYHIMKLKEE 873

Query: 912 FGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEFRLLPK 930
           +G+EPWTF Q +G+AV IP GCPHQVRN +SC KVA+DFVSPENV EC +LT+++RLLP 
Sbjct: 874 YGIEPWTFNQKLGDAVLIPVGCPHQVRNLKSCNKVALDFVSPENVSECLRLTKQYRLLPP 888

BLAST of MC05g0436 vs. TAIR 10
Match: AT3G07610.3 (Transcription factor jumonji (jmjC) domain-containing protein )

HSP 1 Score: 568.5 bits (1464), Expect = 9.6e-162
Identity = 330/804 (41.04%), Postives = 444/804 (55.22%), Query Frame = 0

Query: 192 MCHQCQRNDKSGVVFCLKCHRKRFCYKCIERWYPDRTREDVENACPFCRGHCNCKACLRE 251
           MCHQCQ++D+  V  C  C+ KR+C+ C++ WYP   +EDV   C FC   CNC+ACLR 
Sbjct: 154 MCHQCQKSDRI-VERCQTCNSKRYCHPCLDTWYPLIAKEDVAKKCMFCSSTCNCRACLR- 213

Query: 252 FVEVVPKELDASV------KVERLKFLLYKVLPVLRHIQREQSSELEIEGNIQGAQLTEV 311
            ++   K +++++      KV+  KF+L  +LP L+ I  EQ +E E+E  I G +  EV
Sbjct: 214 -LDTKLKGINSNLIVSEEEKVQASKFILQSLLPHLKGINDEQVAEKEVEAKIYGLKFEEV 273

Query: 312 DVRRIKLDNTERMYCDNCNTSIFNFYRSCFNPNCSFDLCLSCCKELRES----------- 371
             +  K    ER+YCD C TSI++ +R+C   +CSFD+CLSCC E+R             
Sbjct: 274 RPQDAKAFPDERLYCDICKTSIYDLHRNC--KSCSFDICLSCCLEIRNGKALACKEDVSW 333

Query: 372 -------FHPKGSEINICRTIFRNKLSSNSIMSDYL-----------LAGRECQLTSTYK 431
                   +  G E  +      NKL     + D L             GR         
Sbjct: 334 NYINRGLEYEHGQEGKVIEKP-ANKLDDK--LKDKLDGKPDDKPKGKPKGRPKGKPDDKP 393

Query: 432 NGMSSSFQG----------------------WSANPDGSIPCPPKQSGGCGIANLELRRT 491
            G     Q                       W AN  G I C       CG   L L+R 
Sbjct: 394 KGKLKGKQDDKPDDKPDEKPVNTDHMKYPSLWKANEAGIITC------CCGAGELVLKRL 453

Query: 492 FKADWTTKLI-------EGAEGLTSDYKVPDSC-SSEVCPSCYLNSNEVRQAAFRENSHD 551
               W ++L+       E  E L     V + C  S       ++S  + +AA RE S D
Sbjct: 454 LPDGWISELVNRVEKTAEAGELLNLPETVLERCPCSNSDRHIDIDSCNLLKAACREGSED 513

Query: 552 NFLYSPNATDIMDDAVDHFQFHWMKGEPVIVRNVLDKTSGLSWEPMVMCRAFRQTGANVK 611
           N+LYSP+  D+  D + HFQ HW+KGEPVIVRNVL+ TSGLSWEPMVM RA RQ  ++V+
Sbjct: 514 NYLYSPSVWDVQQDDLKHFQHHWVKGEPVIVRNVLEATSGLSWEPMVMHRACRQI-SHVQ 573

Query: 612 FKEETRRVKAIDCFDLCEVEINIHQFFVGYLEGRMYRNGWPEMLKLKDWPSSTSFEERLP 671
                + V A+DC D CEV++N+H+FF GY +GR  R GWP +LKLKDWP +  F++ LP
Sbjct: 574 -HGSLKDVVAVDCLDFCEVKVNLHEFFTGYTDGRYDRMGWPLVLKLKDWPPAKVFKDNLP 633

Query: 672 RHCAEFIAALPYSEYTHPKYGLLNLATKLPEGSLKPDMGPKTYIAYGFQEELSRGDSVTK 731
           RH  EF+ +LP   YTHP  G LNLA KLP+  LKPDMGPKTY+A GF +EL RGDSVTK
Sbjct: 634 RHAEEFLCSLPLKHYTHPVNGPLNLAVKLPQNCLKPDMGPKTYVASGFAQELGRGDSVTK 693

Query: 732 LHCDMSDAVNVLTHTSKVNIKPWQTKIIEKRQK-QFAAEDLSQLYGGIDGLKASKKEILG 791
           LHCDMSDA ++ T     N+ P    ++  R   +F    +  +  GI  LK    E   
Sbjct: 694 LHCDMSDA-DLWTKLYPFNVSPSIAFLVLHRPGIRFLVLLMPNMQPGIGNLKKKHAE--- 753

Query: 792 CTSSQQCGQSSDDTTKIFNENCDSEKASGCNEDNNLRSKGSIMDRADSHLENDEKMEVAM 851
                       D  ++++   + E+               I++ +   ++N E  +   
Sbjct: 754 -----------QDLKELYSSVANKEEMM------------EILENSRQQVQNVETDD--- 813

Query: 852 GGAVWDIFRRQDVPKIAKYLEKHQKEFRHMKNKPVNSLVHPIHDQTIFFNAKHKKQLKEE 911
            GA+WDIFRR+D+PK+  Y+EKH KEFRH+   PV+ +VHPIHDQ  +    H  +LKEE
Sbjct: 814 -GALWDIFRREDIPKLESYIEKHHKEFRHLYCCPVSQVVHPIHDQNFYLTRYHIMKLKEE 873

Query: 912 FGVEPWTFEQFIGEAVFIPAGCPHQVRNRQSCIKVAMDFVSPENVEECFQLTEEFRLLPK 930
           +G+EPWTF Q +G+AV IP GCPHQVRN +SC KVA+DFVSPENV EC +LT+++RLLP 
Sbjct: 874 YGIEPWTFNQKLGDAVLIPVGCPHQVRNLKSCNKVALDFVSPENVSECLRLTKQYRLLPP 910