MC05g0434 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0434
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	aspartyl protease family protein 2-like
Location	MC05: 3218650 .. 3220056 (-)
RNA-Seq Expression	MC05g0434
Synteny	MC05g0434

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATGGAGGCAAAACACACTGCATTTCCCTTTATCTTCTCCCTTCTCGCTCTTCTCTCTCTCTCCGCCGCTCTCTCCGATTTCCAAACCCTAGTTCCCCGCCCTCTTCCCACCTCACCGGCCCTCTCATGGCCGGAATCCGACGCCGATTCCAACTCCTTCAGCGAATCGGAGACCGGCTTCACGTTGCAGCTTCACCATTTGGACGCTCTGTCTCTCAACAGCACGCCGGAGGAGCTCTTCCACACCAGGCTTCAGAGAGACGCCATCCGAGTCCAGAAACTGAGTTCACTCGGTGGCGCTCGGAATCTGAGCCAAGTGAGTGGGACCGGCTTCAGTAGCTCGGTGATCTCGGGACTCGCTCAGGGCAGCGGCGAGTACTTCACGCGCATCGGCGTCGGCACGCCGCCCAAGTATGTCTATATGGTGCTCGACACCGGCAGCGACATCGTCTGGCTCCAGTGCGCCCCCTGCAAGAGTTGCTACTCCCAGACCGACCCAGTTTTCAACCCGGCCAAATCCGGATCCTTCGCCAAGGTCCGTTGCCGGACGCCGTTGTGCAGCCGCCTTGAATCTCCCGGATGCAACCAGCGCCAGACGTGCCTCTACCAGGTTTCTTACGGCGACGGTTCCTATACCACCGGGGAATTCGTCACCGAAACCCTAACCTTCCGTCGGACCAAAGTGGATCGCGTCGCCCTCGGCTGTGGCCACGATAATGAGGGCTTGTTCGTCGGTGCGGCGGGGCTTTTAGGTCTCGGCCGGGGAGGGTTATCGTTTCCGTCGCAAACTGGACGGAGTTTCAACCAGAAGTTCTCTTACTGCTTGGTGGATAGGTCCGCCTCCTCCAAACCCTCCTCCGTCGTCTTCGGCGACTCCGCCGTCTCTCGAACCGCCCGGTTCACTCCTCTTCTCTCAAACCCTAGGCTGGACACCTTTTACTACGTCGAACTGTTGGGGATCAGCGTCGGAGGGACCCCCGTCTCCGGCATCTCCGCTTCACATTTCAAGCTCGACCCGACCGGCAACGGCGGTGTCATCATCGATTGCGGTACATCTGTGACTCGATTGAACCGACCGGCGTACATTGCTCTGCGCGACGCCTTCCGTGCCGGAGCTTCGAGTTTGAAATCAGCGCCCGAATTTTCTCTCTTCGATACTTGCTACGACCTGTCGGGGAAGACGACGGTGAAGGTTCCGACGGTGGTGTTGCATTTCAGAGGCGCTGACGTGTCGTTGCCGGCGTCCAATTATCTGATCCCCGTGGACGGCAGCGGTCGGTTCTGCTTCGCCTTCGCCGGCACAACCAGCGGCCTGTCGATCATCGGGAACATCCAGCAGCAAGGATTTCGGGTCGTCTACGATTTGGCGGGTTCTCGGGTCGGGTTCTCTCCACGTGGATGCGCC

mRNA sequence

AAAATGGAGGCAAAACACACTGCATTTCCCTTTATCTTCTCCCTTCTCGCTCTTCTCTCTCTCTCCGCCGCTCTCTCCGATTTCCAAACCCTAGTTCCCCGCCCTCTTCCCACCTCACCGGCCCTCTCATGGCCGGAATCCGACGCCGATTCCAACTCCTTCAGCGAATCGGAGACCGGCTTCACGTTGCAGCTTCACCATTTGGACGCTCTGTCTCTCAACAGCACGCCGGAGGAGCTCTTCCACACCAGGCTTCAGAGAGACGCCATCCGAGTCCAGAAACTGAGTTCACTCGGTGGCGCTCGGAATCTGAGCCAAGTGAGTGGGACCGGCTTCAGTAGCTCGGTGATCTCGGGACTCGCTCAGGGCAGCGGCGAGTACTTCACGCGCATCGGCGTCGGCACGCCGCCCAAGTATGTCTATATGGTGCTCGACACCGGCAGCGACATCGTCTGGCTCCAGTGCGCCCCCTGCAAGAGTTGCTACTCCCAGACCGACCCAGTTTTCAACCCGGCCAAATCCGGATCCTTCGCCAAGGTCCGTTGCCGGACGCCGTTGTGCAGCCGCCTTGAATCTCCCGGATGCAACCAGCGCCAGACGTGCCTCTACCAGGTTTCTTACGGCGACGGTTCCTATACCACCGGGGAATTCGTCACCGAAACCCTAACCTTCCGTCGGACCAAAGTGGATCGCGTCGCCCTCGGCTGTGGCCACGATAATGAGGGCTTGTTCGTCGGTGCGGCGGGGCTTTTAGGTCTCGGCCGGGGAGGGTTATCGTTTCCGTCGCAAACTGGACGGAGTTTCAACCAGAAGTTCTCTTACTGCTTGGTGGATAGGTCCGCCTCCTCCAAACCCTCCTCCGTCGTCTTCGGCGACTCCGCCGTCTCTCGAACCGCCCGGTTCACTCCTCTTCTCTCAAACCCTAGGCTGGACACCTTTTACTACGTCGAACTGTTGGGGATCAGCGTCGGAGGGACCCCCGTCTCCGGCATCTCCGCTTCACATTTCAAGCTCGACCCGACCGGCAACGGCGGTGTCATCATCGATTGCGGTACATCTGTGACTCGATTGAACCGACCGGCGTACATTGCTCTGCGCGACGCCTTCCGTGCCGGAGCTTCGAGTTTGAAATCAGCGCCCGAATTTTCTCTCTTCGATACTTGCTACGACCTGTCGGGGAAGACGACGGTGAAGGTTCCGACGGTGGTGTTGCATTTCAGAGGCGCTGACGTGTCGTTGCCGGCGTCCAATTATCTGATCCCCGTGGACGGCAGCGGTCGGTTCTGCTTCGCCTTCGCCGGCACAACCAGCGGCCTGTCGATCATCGGGAACATCCAGCAGCAAGGATTTCGGGTCGTCTACGATTTGGCGGGTTCTCGGGTCGGGTTCTCTCCACGTGGATGCGCC

Coding sequence (CDS)

AAAATGGAGGCAAAACACACTGCATTTCCCTTTATCTTCTCCCTTCTCGCTCTTCTCTCTCTCTCCGCCGCTCTCTCCGATTTCCAAACCCTAGTTCCCCGCCCTCTTCCCACCTCACCGGCCCTCTCATGGCCGGAATCCGACGCCGATTCCAACTCCTTCAGCGAATCGGAGACCGGCTTCACGTTGCAGCTTCACCATTTGGACGCTCTGTCTCTCAACAGCACGCCGGAGGAGCTCTTCCACACCAGGCTTCAGAGAGACGCCATCCGAGTCCAGAAACTGAGTTCACTCGGTGGCGCTCGGAATCTGAGCCAAGTGAGTGGGACCGGCTTCAGTAGCTCGGTGATCTCGGGACTCGCTCAGGGCAGCGGCGAGTACTTCACGCGCATCGGCGTCGGCACGCCGCCCAAGTATGTCTATATGGTGCTCGACACCGGCAGCGACATCGTCTGGCTCCAGTGCGCCCCCTGCAAGAGTTGCTACTCCCAGACCGACCCAGTTTTCAACCCGGCCAAATCCGGATCCTTCGCCAAGGTCCGTTGCCGGACGCCGTTGTGCAGCCGCCTTGAATCTCCCGGATGCAACCAGCGCCAGACGTGCCTCTACCAGGTTTCTTACGGCGACGGTTCCTATACCACCGGGGAATTCGTCACCGAAACCCTAACCTTCCGTCGGACCAAAGTGGATCGCGTCGCCCTCGGCTGTGGCCACGATAATGAGGGCTTGTTCGTCGGTGCGGCGGGGCTTTTAGGTCTCGGCCGGGGAGGGTTATCGTTTCCGTCGCAAACTGGACGGAGTTTCAACCAGAAGTTCTCTTACTGCTTGGTGGATAGGTCCGCCTCCTCCAAACCCTCCTCCGTCGTCTTCGGCGACTCCGCCGTCTCTCGAACCGCCCGGTTCACTCCTCTTCTCTCAAACCCTAGGCTGGACACCTTTTACTACGTCGAACTGTTGGGGATCAGCGTCGGAGGGACCCCCGTCTCCGGCATCTCCGCTTCACATTTCAAGCTCGACCCGACCGGCAACGGCGGTGTCATCATCGATTGCGGTACATCTGTGACTCGATTGAACCGACCGGCGTACATTGCTCTGCGCGACGCCTTCCGTGCCGGAGCTTCGAGTTTGAAATCAGCGCCCGAATTTTCTCTCTTCGATACTTGCTACGACCTGTCGGGGAAGACGACGGTGAAGGTTCCGACGGTGGTGTTGCATTTCAGAGGCGCTGACGTGTCGTTGCCGGCGTCCAATTATCTGATCCCCGTGGACGGCAGCGGTCGGTTCTGCTTCGCCTTCGCCGGCACAACCAGCGGCCTGTCGATCATCGGGAACATCCAGCAGCAAGGATTTCGGGTCGTCTACGATTTGGCGGGTTCTCGGGTCGGGTTCTCTCCACGTGGATGCGCC

Protein sequence

KMEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSFSESETGFTLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSLGGARNLSQVSGTGFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSGSFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAVSRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA

Homology

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BLAST of MC05g0434 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.3e-183
Identity = 336/481 (69.85%), Postives = 384/481 (79.83%), Query Frame = 0


Query: 5   KHTAFPFIFSLLALLSLSAALSDFQTLVP--RPLP-TSPALSWPESDA--------DSNS 64
           K   F   F  L+L S S +L  FQTL P    LP  SP    P+SD+        +S S
Sbjct: 6   KALLFSLCFFFLSLPSFS-SLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGS 65

Query: 65  FSESETGFTLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSLGG---ARNLSQVSGT 124
            SES +  TL L H+DALS N TP+ELF +RLQRD+ RV+ +++L      RN++     
Sbjct: 66  DSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRP 125

Query: 125 -GFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVF 184
            GFSSSV+SGL+QGSGEYFTR+GVGTP +YVYMVLDTGSDIVWLQCAPC+ CYSQ+DP+F
Sbjct: 126 GGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIF 185

Query: 185 NPAKSGSFAKVRCRTPLCSRLESPGCN-QRQTCLYQVSYGDGSYTTGEFVTETLTFRRTK 244
           +P KS ++A + C +P C RL+S GCN +R+TCLYQVSYGDGS+T G+F TETLTFRR +
Sbjct: 186 DPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNR 245

Query: 245 VDRVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSV 304
           V  VALGCGHDNEGLFVGAAGLLGLG+G LSFP QTG  FNQKFSYCLVDRSASSKPSSV
Sbjct: 246 VKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSV 305

Query: 305 VFGDSAVSRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVII 364
           VFG++AVSR ARFTPLLSNP+LDTFYYV LLGISVGGT V G++AS FKLD  GNGGVII
Sbjct: 306 VFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVII 365

Query: 365 DCGTSVTRLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRG 424
           D GTSVTRL RPAYIA+RDAFR GA +LK AP+FSLFDTC+DLS    VKVPTVVLHFRG
Sbjct: 366 DSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRG 425

Query: 425 ADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 470
           ADVSLPA+NYLIPVD +G+FCFAFAGT  GLSIIGNIQQQGFRVVYDLA SRVGF+P GC
Sbjct: 426 ADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 485

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BLAST of MC05g0434 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.6e-109
Identity = 221/470 (47.02%), Postives = 294/470 (62.55%), Query Frame = 0


Query: 9   FPFIFSLLALLSLSAALS--DFQTLVPRPLPTSPALSWPESDADSNSFS-ESETGFTLQL 68
           F F   L   LS S+++S  DFQ +     P +   + P  D ++  FS ES + +TL+L
Sbjct: 6   FFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLP--DFNNTHFSDESSSKYTLRL 65

Query: 69  HHLDALS--LNSTPEELFHTRLQRDAIRV----QKLSSLGGARNLSQVSGTGFSSSVISG 128
            H D              H R++RD  RV    +++S      + S+     F S ++SG
Sbjct: 66  LHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSG 125

Query: 129 LAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSGSFAK 188
           + QGSGEYF RIGVG+PP+  YMV+D+GSD+VW+QC PCK CY Q+DPVF+PAKSGS+  
Sbjct: 126 MDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTG 185

Query: 189 VRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALGCGHD 248
           V C + +C R+E+ GC+    C Y+V YGDGSYT G    ETLTF +T V  VA+GCGH 
Sbjct: 186 VSCGSSVCDRIENSGCHS-GGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHR 245

Query: 249 NEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAVSRTA 308
           N G+F+GAAGLLG+G G +SF  Q        F YCLV R   S   S+VFG  A+   A
Sbjct: 246 NRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREALPVGA 305

Query: 309 RFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVTRLNR 368
            + PL+ NPR  +FYYV L G+ VGG  +  +    F L  TG+GGV++D GT+VTRL  
Sbjct: 306 SWVPLVRNPRAPSFYYVGLKGLGVGGVRIP-LPDGVFDLTETGDGGVVMDTGTAVTRLPT 365

Query: 369 PAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHF-RGADVSLPASNY 428
            AY+A RD F++  ++L  A   S+FDTCYDLSG  +V+VPTV  +F  G  ++LPA N+
Sbjct: 366 AAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNF 425

Query: 429 LIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 469
           L+PVD SG +CFAFA + +GLSIIGNIQQ+G +V +D A   VGF P  C
Sbjct: 426 LMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of MC05g0434 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.0e-108
Identity = 237/506 (46.84%), Postives = 304/506 (60.08%), Query Frame = 0


Query: 8   AFPFIFSLLALLSLS-------------------------AALSDFQTLVP-RPLPTSPA 67
           AFP   SLLA+++LS                         ++L   QT++   P  +S  
Sbjct: 2   AFPRFLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLT 61

Query: 68  LSWPESDADSNSFSESETGFTLQLHHLDAL--SLNSTPEELFHTRLQRDAIRVQKL---- 127
            + PES +D   F  S +  +L+LH  D    S +   + L  +RL+RD+ RV  +    
Sbjct: 62  TTKPESLSDP-VFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKI 121

Query: 128 ---------SSLGGARNL-SQVSGTGFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLD 187
                    S L    N  ++      ++ V+SG +QGSGEYF+RIGVGTP K +Y+VLD
Sbjct: 122 RFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLD 181

Query: 188 TGSDIVWLQCAPCKSCYSQTDPVFNPAKSGSFAKVRCRTPLCSRLESPGCNQRQTCLYQV 247
           TGSD+ W+QC PC  CY Q+DPVFNP  S ++  + C  P CS LE+  C   + CLYQV
Sbjct: 182 TGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNK-CLYQV 241

Query: 248 SYGDGSYTTGEFVTETLTFRRT-KVDRVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQT 307
           SYGDGS+T GE  T+T+TF  + K++ VALGCGHDNEGLF GAAGLLGLG G LS  +Q 
Sbjct: 242 SYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM 301

Query: 308 GRSFNQKFSYCLVDRSASSKPSSVVFGDSAVSRTARFTPLLSNPRLDTFYYVELLGISVG 367
             +    FSYCLVDR  S K SS+ F    +       PLL N ++DTFYYV L G SVG
Sbjct: 302 KAT---SFSYCLVDRD-SGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVG 361

Query: 368 GTPVSGISASHFKLDPTGNGGVIIDCGTSVTRLNRPAYIALRDAF-RAGASSLKSAPEFS 427
           G  V  +  + F +D +G+GGVI+DCGT+VTRL   AY +LRDAF +   +  K +   S
Sbjct: 362 GEKVV-LPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSIS 421

Query: 428 LFDTCYDLSGKTTVKVPTVVLHFRGA-DVSLPASNYLIPVDGSGRFCFAFAGTTSGLSII 469
           LFDTCYD S  +TVKVPTV  HF G   + LPA NYLIPVD SG FCFAFA T+S LSII
Sbjct: 422 LFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSII 481

[Close]

BLAST of MC05g0434 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 5.7e-75
Identity = 173/433 (39.95%), Postives = 240/433 (55.43%), Query Frame = 0


Query: 44  WPESDADSNSFSESETGFTLQLHHL-DALSLNSTPEELFHTR-LQRDAIRVQKL---SSL 103
           +P S +   S   S T  +L++ H+  A S  S+   + H   ++RD  RV+ +    S 
Sbjct: 46  FPSSSSCVPSSKASNTKSSLRVVHMHGACSHLSSDARVDHDEIIRRDQARVESIYSKLSK 105

Query: 104 GGARNLSQVSGTGFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPC 163
             A  +S+   T   +   SG+  GSG Y   IG+GTP   + +V DTGSD+ W QC PC
Sbjct: 106 NSANEVSEAKSTELPAK--SGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPC 165

Query: 164 -KSCYSQTDPVFNPAKSGSFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEF 223
             SCYSQ +P FNP+ S ++  V C +P+C   ES  C+    C+Y + YGD S+T G  
Sbjct: 166 LGSCYSQKEPKFNPSSSSTYQNVSCSSPMCEDAES--CS-ASNCVYSIVYGDKSFTQGFL 225

Query: 224 VTETLTFRRTKV-DRVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCL 283
             E  T   + V + V  GCG +N+GLF G AGLLGLG G LS P+QT  ++N  FSYCL
Sbjct: 226 AKEKFTLTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCL 285

Query: 284 VDRSASSKPSSVVFGDSAVSRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHF 343
              +++S    + FG + +S + +FTP+ S P     Y ++++GISVG   ++ I+ + F
Sbjct: 286 PSFTSNS-TGHLTFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELA-ITPNSF 345

Query: 344 KLDPTGNGGVIIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTT 403
             +     G IID GT  TRL    Y  LR  F+   SS KS   + LFDTCYD +G  T
Sbjct: 346 STE-----GAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDT 405

Query: 404 VKVPTVVLHFRGAD-VSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYD 463
           V  PT+   F G+  V L  S   +P+  S + C AFAG     +I GN+QQ    VVYD
Sbjct: 406 VTYPTIAFSFAGSTVVELDGSGISLPIKIS-QVCLAFAGNDDLPAIFGNVQQTTLDVVYD 464

Query: 464 LAGSRVGFSPRGC 469
           +AG RVGF+P GC
Sbjct: 466 VAGGRVGFAPNGC 464

[Close]

BLAST of MC05g0434 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.2e-72
Identity = 160/397 (40.30%), Postives = 220/397 (55.42%), Query Frame = 0


Query: 85  LQRDAIRV----QKLSSLGGARNLSQVSGTGFSSSVISGLAQGSGEYFTRIGVGTPPKYV 144
           L+ D  RV     KLS      ++S+   T   +    G   GSG Y   +G+GTP   +
Sbjct: 88  LRLDQARVNSIHSKLSKKLATDHVSESKSTDLPAK--DGSTLGSGNYIVTVGLGTPKNDL 147

Query: 145 YMVLDTGSDIVWLQCAPC-KSCYSQTDPVFNPAKSGSFAKVRCRTPLCSRLES----PGC 204
            ++ DTGSD+ W QC PC ++CY Q +P+FNP+KS S+  V C +  C  L S     G 
Sbjct: 148 SLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGS 207

Query: 205 NQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKV-DRVALGCGHDNEGLFVGAAGLLGLG 264
                C+Y + YGD S++ G    E  T   + V D V  GCG +N+GLF G AGLLGLG
Sbjct: 208 CSASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLG 267

Query: 265 RGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAVSRTARFTPLLSNPRLDTFY 324
           R  LSFPSQT  ++N+ FSYCL   S++S    + FG + +SR+ +FTP+ +     +FY
Sbjct: 268 RDKLSFPSQTATAYNKIFSYCL--PSSASYTGHLTFGSAGISRSVKFTPISTITDGTSFY 327

Query: 325 YVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVTRLNRPAYIALRDAFRAGAS 384
            + ++ I+VGG  +  I ++ F        G +ID GT +TRL   AY ALR +F+A  S
Sbjct: 328 GLNIVAITVGGQKLP-IPSTVF-----STPGALIDSGTVITRLPPKAYAALRSSFKAKMS 387

Query: 385 SLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASNYLIPVDGSGRFCFAFAG 444
              +    S+ DTC+DLSG  TV +P V   F G  V    S  +  V    + C AFAG
Sbjct: 388 KYPTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAG 447

Query: 445 TT--SGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 470
            +  S  +I GN+QQQ   VVYD AG RVGF+P GC+
Sbjct: 448 NSDDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGCS 474

[Close]

BLAST of MC05g0434 vs. NCBI nr
Match: XP_022945440.1 (aspartyl protease family protein 2-like [Cucurbita moschata])

HSP 1 Score: 830 bits (2144), Expect = 1.38e-301
Identity = 423/474 (89.24%), Postives = 438/474 (92.41%), Query Frame = 0


Query: 2   MEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSFSESETGF 61
           MEAK +AFPFI  LL LLSLS A SDFQTL+P+ LP SP+L  PES  DS SF  SE G 
Sbjct: 1   MEAKTSAFPFIGFLLTLLSLSTAFSDFQTLIPKSLPASPSLLSPESATDSESFISSEAGL 60

Query: 62  TLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSL-GGARNLSQVSGT-----GFSSS 121
            LQLHHLDALSLN TPEELFH RLQRDA+RV KLSSL GG++NLSQ SGT     GFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 122 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSG 181
           VISGLAQGSGEYFTRIGVGTPPKY+YMVLDTGSDIVWLQCAPCK+CYSQTDPVFNP KS 
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 182 SFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALG 241
           S++KV CRTPLC RLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKV+RVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 242 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 301
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 302 SRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 361
           SRTARFTPLL+NPRLDTFYYVELLGISVGGTPVSGISASHFKLDP GNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPNGNGGVIIDCGTSVT 360

Query: 362 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 421
           RLNRPAYIALRDAFRAGASSLK APEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 422 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 469
           SNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

[Close]

BLAST of MC05g0434 vs. NCBI nr
Match: XP_023538763.1 (aspartyl protease family protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 830 bits (2144), Expect = 1.38e-301
Identity = 424/474 (89.45%), Postives = 438/474 (92.41%), Query Frame = 0


Query: 2   MEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSFSESETGF 61
           MEAK +AFPFI  LL LLSLS A SDFQTLVP+PLP SP+L  PES  DS SF  SE G 
Sbjct: 1   MEAKTSAFPFIGFLLTLLSLSTAFSDFQTLVPKPLPPSPSLFSPESATDSESFISSEAGL 60

Query: 62  TLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSL-GGARNLSQVSGT-----GFSSS 121
            LQLHHLDALSLN TPEELFH RLQRDA+RV KLSSL GG++NL+Q SGT     GFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLRGGSQNLTQASGTSHGTTGFSSS 120

Query: 122 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSG 181
           VISGLAQGSGEYFTRIGVGTPPKY+YMVLDTGSDIVWLQCAPCK+CYSQTDPVFNP KS 
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 182 SFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALG 241
           S++KV CRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKV+RVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 242 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 301
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 302 SRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 361
           SRTARFTPLL+NPRLDTFYYVELLGISVGGTPVSGISASHFKLD  GNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDSNGNGGVIIDCGTSVT 360

Query: 362 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 421
           RLNRPAYIALRDAFRAGASSLK APEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 422 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 469
           SNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

[Close]

BLAST of MC05g0434 vs. NCBI nr
Match: KAG6597078.1 (Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 828 bits (2139), Expect = 7.99e-301
Identity = 423/474 (89.24%), Postives = 438/474 (92.41%), Query Frame = 0


Query: 2   MEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSFSESETGF 61
           MEAK +AFPFI SLL LLSLSAA SDFQTL+P+ LP S +L  PES  DS  F  SE G 
Sbjct: 1   MEAKTSAFPFIGSLLTLLSLSAAFSDFQTLIPKSLPASHSLLSPESATDSEYFISSEAGL 60

Query: 62  TLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSL-GGARNLSQVSGT-----GFSSS 121
            LQLHHLDALSLN TPEELFH RLQRDA+RV KLSSL GG++NLSQ SGT     GFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 122 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSG 181
           VISGLAQGSGEYFTRIGVGTPPKY+YMVLDTGSDIVWLQCAPCK+CYSQTDPVFNP KS 
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 182 SFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALG 241
           S++KV CRTPLC RLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKV+RVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 242 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 301
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 302 SRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 361
           SRTARFTPLL+NPRLDTFYYVELLGISVGGTPVSGISASHFKLDP GNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPNGNGGVIIDCGTSVT 360

Query: 362 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 421
           RLNRPAYIALRDAFRAGASSLK APEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 422 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 469
           SNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

[Close]

BLAST of MC05g0434 vs. NCBI nr
Match: XP_022974939.1 (aspartyl protease family protein 2-like [Cucurbita maxima])

HSP 1 Score: 826 bits (2133), Expect = 6.31e-300
Identity = 422/474 (89.03%), Postives = 437/474 (92.19%), Query Frame = 0


Query: 2   MEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSFSESETGF 61
           MEAK +A PFI  LL LLSLS A SDFQTL+P+PLP SP+ S PESD DS SF  SE G 
Sbjct: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSFS-PESDTDSESFISSEAGL 60

Query: 62  TLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSL-GGARNLSQVSGT-----GFSSS 121
            LQLHHLDALSLN TPEELFH RLQRDA+RV KLSSL GG++NLSQ SGT     GFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 122 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSG 181
           VISGLAQGSGEYFTRIGVGTPPKY+YMVLDTGSDIVWLQCAPCK+CYSQTDPVFNP KS 
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 182 SFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALG 241
           S++KV CRTPLC RLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKV+RVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 242 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 301
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 302 SRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 361
           SRTARFTPLL+NPRLDTFYYVELLGISVGGTPV GISASHFKLD  GNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVFGISASHFKLDSNGNGGVIIDCGTSVT 360

Query: 362 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 421
           RLNRPAYIALRDAFRAGASSLK APEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 422 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 469
           SNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 473

[Close]

BLAST of MC05g0434 vs. NCBI nr
Match: XP_022975090.1 (aspartyl protease family protein 2-like [Cucurbita maxima])

HSP 1 Score: 826 bits (2133), Expect = 6.55e-300
Identity = 421/474 (88.82%), Postives = 437/474 (92.19%), Query Frame = 0


Query: 2   MEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSFSESETGF 61
           MEAK +A PFI  LL LLSLS A SDFQTL+P+PLP SP+L  PESD DS SF  SE G 
Sbjct: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60

Query: 62  TLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSL-GGARNLSQVSGT-----GFSSS 121
            LQLHHLDALSLN TPEELFH RLQRDA+RV KLSSL GG++NLSQ SGT     GFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 122 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSG 181
           VISGLAQGSGEYFTRIGVGTPPKY+YMVLDTGSDIVWLQCAPCK+CYSQTDPVFNP KS 
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 182 SFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALG 241
           S++KV CRTPLC RLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKV+RVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 242 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 301
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 302 SRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 361
           SRTARFTPLL+NPRLDTFYYVELLGISVGGTPVS ISASHFKLD  GNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360

Query: 362 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 421
           RLNRPAYIALR+AFRAGASSLK APEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 422 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 469
           SNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

[Close]

BLAST of MC05g0434 vs. ExPASy TrEMBL
Match: A0A6J1G0Y1 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111449669 PE=3 SV=1)

HSP 1 Score: 830 bits (2144), Expect = 6.70e-302
Identity = 423/474 (89.24%), Postives = 438/474 (92.41%), Query Frame = 0


Query: 2   MEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSFSESETGF 61
           MEAK +AFPFI  LL LLSLS A SDFQTL+P+ LP SP+L  PES  DS SF  SE G 
Sbjct: 1   MEAKTSAFPFIGFLLTLLSLSTAFSDFQTLIPKSLPASPSLLSPESATDSESFISSEAGL 60

Query: 62  TLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSL-GGARNLSQVSGT-----GFSSS 121
            LQLHHLDALSLN TPEELFH RLQRDA+RV KLSSL GG++NLSQ SGT     GFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 122 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSG 181
           VISGLAQGSGEYFTRIGVGTPPKY+YMVLDTGSDIVWLQCAPCK+CYSQTDPVFNP KS 
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 182 SFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALG 241
           S++KV CRTPLC RLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKV+RVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 242 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 301
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 302 SRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 361
           SRTARFTPLL+NPRLDTFYYVELLGISVGGTPVSGISASHFKLDP GNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPNGNGGVIIDCGTSVT 360

Query: 362 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 421
           RLNRPAYIALRDAFRAGASSLK APEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 422 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 469
           SNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

[Close]

BLAST of MC05g0434 vs. ExPASy TrEMBL
Match: A0A6J1IJ02 (aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111473763 PE=3 SV=1)

HSP 1 Score: 826 bits (2133), Expect = 3.06e-300
Identity = 422/474 (89.03%), Postives = 437/474 (92.19%), Query Frame = 0


Query: 2   MEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSFSESETGF 61
           MEAK +A PFI  LL LLSLS A SDFQTL+P+PLP SP+ S PESD DS SF  SE G 
Sbjct: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSFS-PESDTDSESFISSEAGL 60

Query: 62  TLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSL-GGARNLSQVSGT-----GFSSS 121
            LQLHHLDALSLN TPEELFH RLQRDA+RV KLSSL GG++NLSQ SGT     GFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 122 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSG 181
           VISGLAQGSGEYFTRIGVGTPPKY+YMVLDTGSDIVWLQCAPCK+CYSQTDPVFNP KS 
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 182 SFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALG 241
           S++KV CRTPLC RLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKV+RVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 242 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 301
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 302 SRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 361
           SRTARFTPLL+NPRLDTFYYVELLGISVGGTPV GISASHFKLD  GNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVFGISASHFKLDSNGNGGVIIDCGTSVT 360

Query: 362 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 421
           RLNRPAYIALRDAFRAGASSLK APEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 422 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 469
           SNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 473

[Close]

BLAST of MC05g0434 vs. ExPASy TrEMBL
Match: A0A6J1IFR8 (aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111474062 PE=3 SV=1)

HSP 1 Score: 826 bits (2133), Expect = 3.17e-300
Identity = 421/474 (88.82%), Postives = 437/474 (92.19%), Query Frame = 0


Query: 2   MEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSFSESETGF 61
           MEAK +A PFI  LL LLSLS A SDFQTL+P+PLP SP+L  PESD DS SF  SE G 
Sbjct: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60

Query: 62  TLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSL-GGARNLSQVSGT-----GFSSS 121
            LQLHHLDALSLN TPEELFH RLQRDA+RV KLSSL GG++NLSQ SGT     GFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 122 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSG 181
           VISGLAQGSGEYFTRIGVGTPPKY+YMVLDTGSDIVWLQCAPCK+CYSQTDPVFNP KS 
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 182 SFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALG 241
           S++KV CRTPLC RLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKV+RVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 242 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 301
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 302 SRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 361
           SRTARFTPLL+NPRLDTFYYVELLGISVGGTPVS ISASHFKLD  GNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360

Query: 362 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 421
           RLNRPAYIALR+AFRAGASSLK APEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 422 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 469
           SNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

[Close]

BLAST of MC05g0434 vs. ExPASy TrEMBL
Match: A0A6J1IY26 (aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479557 PE=3 SV=1)

HSP 1 Score: 817 bits (2110), Expect = 9.74e-297
Identity = 417/473 (88.16%), Postives = 434/473 (91.75%), Query Frame = 0


Query: 2   MEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSFS----ES 61
           M AK + FPFIF LL LL LS A SDFQTLVPRPLPTSP+   PES   S+SFS    ES
Sbjct: 1   MVAKTSPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATES 60

Query: 62  ETGFTLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSLGGA-RNLSQVSGTGFSSSV 121
           E G  L LHHLD+LSL+ TPEELFH RLQRDA+RV KLS L  A RN+S+ SGTGFSSSV
Sbjct: 61  EPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGTGFSSSV 120

Query: 122 ISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSGS 181
           ISGLAQGSGEYFTRIGVGTPP+YVY+VLDTGSDIVWLQCAPCK+CYSQTDPVF+P KSGS
Sbjct: 121 ISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGS 180

Query: 182 FAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALGC 241
           F+KV CRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKV+RVALGC
Sbjct: 181 FSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGC 240

Query: 242 GHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAVS 301
           GHDNEGLFVGAAGLLGLGRGGLSFPSQTGR+FNQKFSYCLVDRSASSKPSSVVFG+SAVS
Sbjct: 241 GHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAVS 300

Query: 302 RTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVTR 361
           RTARFTPLL+NPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTSVTR
Sbjct: 301 RTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTR 360

Query: 362 LNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPAS 421
           LNRPAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLPAS
Sbjct: 361 LNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPAS 420

Query: 422 NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 469
           NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 473

[Close]

BLAST of MC05g0434 vs. ExPASy TrEMBL
Match: A0A6J1EFG4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432036 PE=3 SV=1)

HSP 1 Score: 814 bits (2103), Expect = 1.18e-295
Identity = 416/474 (87.76%), Postives = 434/474 (91.56%), Query Frame = 0


Query: 2   MEAKHTAFPFIFSLLALLSLSAALSDFQTLVPRPLPTSPALSWPESDADSNSF-----SE 61
           M AK + F FIF LL LLSLS A SDFQTLVPRPLPTSP+   PES+ DS+SF     +E
Sbjct: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60

Query: 62  SETGFTLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSLGGAR-NLSQVSGTGFSSS 121
           SE G  L LHHLD+LSL+ TPEELFH RLQRDA+RV KLS L     N+S+ SGTGFSSS
Sbjct: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS 120

Query: 122 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSG 181
           VISGLAQGSGEYFTRIGVGTPP+YVYMVLDTGSDIVWLQCAPCK+CYSQTDPVF+P KSG
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180

Query: 182 SFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALG 241
           SF+KV CRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKV+RVALG
Sbjct: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 242 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 301
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGR+FNQKFSYCLVDRSASSKPSSVVFG+SAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300

Query: 302 SRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 361
           SRTARFTPLL+NPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360

Query: 362 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 421
           RLNRPAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420

Query: 422 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 469
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

[Close]

BLAST of MC05g0434 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 644.0 bits (1660), Expect = 9.1e-185
Identity = 336/481 (69.85%), Postives = 384/481 (79.83%), Query Frame = 0


Query: 5   KHTAFPFIFSLLALLSLSAALSDFQTLVP--RPLP-TSPALSWPESDA--------DSNS 64
           K   F   F  L+L S S +L  FQTL P    LP  SP    P+SD+        +S S
Sbjct: 6   KALLFSLCFFFLSLPSFS-SLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGS 65

Query: 65  FSESETGFTLQLHHLDALSLNSTPEELFHTRLQRDAIRVQKLSSLGG---ARNLSQVSGT 124
            SES +  TL L H+DALS N TP+ELF +RLQRD+ RV+ +++L      RN++     
Sbjct: 66  DSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRP 125

Query: 125 -GFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVF 184
            GFSSSV+SGL+QGSGEYFTR+GVGTP +YVYMVLDTGSDIVWLQCAPC+ CYSQ+DP+F
Sbjct: 126 GGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIF 185

Query: 185 NPAKSGSFAKVRCRTPLCSRLESPGCN-QRQTCLYQVSYGDGSYTTGEFVTETLTFRRTK 244
           +P KS ++A + C +P C RL+S GCN +R+TCLYQVSYGDGS+T G+F TETLTFRR +
Sbjct: 186 DPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNR 245

Query: 245 VDRVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSV 304
           V  VALGCGHDNEGLFVGAAGLLGLG+G LSFP QTG  FNQKFSYCLVDRSASSKPSSV
Sbjct: 246 VKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSV 305

Query: 305 VFGDSAVSRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVII 364
           VFG++AVSR ARFTPLLSNP+LDTFYYV LLGISVGGT V G++AS FKLD  GNGGVII
Sbjct: 306 VFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVII 365

Query: 365 DCGTSVTRLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRG 424
           D GTSVTRL RPAYIA+RDAFR GA +LK AP+FSLFDTC+DLS    VKVPTVVLHFRG
Sbjct: 366 DSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRG 425

Query: 425 ADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 470
           ADVSLPA+NYLIPVD +G+FCFAFAGT  GLSIIGNIQQQGFRVVYDLA SRVGF+P GC
Sbjct: 426 ADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 485

[Close]

BLAST of MC05g0434 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 592.4 bits (1526), Expect = 3.1e-169
Identity = 308/473 (65.12%), Postives = 367/473 (77.59%), Query Frame = 0


Query: 13  FSLLALLSL-SAALSDFQTLVPRPLPTSPALSWPESDA-DSNSFSESETGFTLQLHHLDA 72
           FS+ A+L   S+A S +QTLV   LP+S  LSWPES++    S SES T  ++ L H+DA
Sbjct: 11  FSVFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLSESTTSLSVHLSHVDA 70

Query: 73  LS--LNSTPEELFHTRLQRDAIRVQKLSSLG------GARNLSQVSGTGFSSSVISGLAQ 132
           LS   +++P +LF+ RLQRD++RV+ ++SL        A   +  +  GFS +VISGL+Q
Sbjct: 71  LSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTAGGFSGAVISGLSQ 130

Query: 133 GSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSGSFAKVRC 192
           GSGEYF R+GVGTP   VYMVLDTGSD+VWLQC+PCK+CY+QTD +F+P KS +FA V C
Sbjct: 131 GSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATVPC 190

Query: 193 RTPLCSRL-ESPGCNQR--QTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALGCGHD 252
            + LC RL +S  C  R  +TCLYQVSYGDGS+T G+F TETLTF   +VD V LGCGHD
Sbjct: 191 GSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCGHD 250

Query: 253 NEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDR----SASSKPSSVVFGDSAV 312
           NEGLFVGAAGLLGLGRGGLSFPSQT   +N KFSYCLVDR    S+S  PS++VFG++AV
Sbjct: 251 NEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAV 310

Query: 313 SRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 372
            +T+ FTPLL+NP+LDTFYY++LLGISVGG+ V G+S S FKLD TGNGGVIID GTSVT
Sbjct: 311 PKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVT 370

Query: 373 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 432
           RL +PAY+ALRDAFR GA+ LK AP +SLFDTC+DLSG TTVKVPTVV HF G +VSLPA
Sbjct: 371 RLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGGEVSLPA 430

Query: 433 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 469
           SNYLIPV+  GRFCFAFAGT   LSIIGNIQQQGFRV YDL GSRVGF  R C
Sbjct: 431 SNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of MC05g0434 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.7e-112
Identity = 221/428 (51.64%), Postives = 276/428 (64.49%), Query Frame = 0


Query: 57  SETGFTLQLHHLDAL--SLNSTPEELFHTRLQRDAIRVQKL-------------SSLGGA 116
           + + F+LQLH   ++  + +S  + L   RL RD  RV+ L             + L   
Sbjct: 63  ASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKPI 122

Query: 117 RNLSQVSGTGFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSC 176
             +         + +ISG  QGSGEYFTR+G+G P + VYMVLDTGSD+ WLQC PC  C
Sbjct: 123 STMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADC 182

Query: 177 YSQTDPVFNPAKSGSFAKVRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTET 236
           Y QT+P+F P+ S S+  + C TP C+ LE   C +  TCLY+VSYGDGSYT G+F TET
Sbjct: 183 YHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSEC-RNATCLYEVSYGDGSYTVGDFATET 242

Query: 237 LTFRRTKVDRVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSA 296
           LT   T V  VA+GCGH NEGLFVGAAGLLGLG G L+ PSQ   +    FSYCLVDR +
Sbjct: 243 LTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTT---SFSYCLVDRDS 302

Query: 297 SSKPSSVVFGDSAVSRTARFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPT 356
            S  S+V FG S +S  A   PLL N +LDTFYY+ L GISVGG  +  I  S F++D +
Sbjct: 303 DS-ASTVDFGTS-LSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQ-IPQSSFEMDES 362

Query: 357 GNGGVIIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPT 416
           G+GG+IID GT+VTRL    Y +LRD+F  G   L+ A   ++FDTCY+LS KTTV+VPT
Sbjct: 363 GSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPT 422

Query: 417 VVLHFRGAD-VSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSR 469
           V  HF G   ++LPA NY+IPVD  G FC AFA T S L+IIGN+QQQG RV +DLA S 
Sbjct: 423 VAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSL 482

[Close]

BLAST of MC05g0434 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 396.4 bits (1017), Expect = 3.3e-110
Identity = 221/470 (47.02%), Postives = 294/470 (62.55%), Query Frame = 0


Query: 9   FPFIFSLLALLSLSAALS--DFQTLVPRPLPTSPALSWPESDADSNSFS-ESETGFTLQL 68
           F F   L   LS S+++S  DFQ +     P +   + P  D ++  FS ES + +TL+L
Sbjct: 6   FFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLP--DFNNTHFSDESSSKYTLRL 65

Query: 69  HHLDALS--LNSTPEELFHTRLQRDAIRV----QKLSSLGGARNLSQVSGTGFSSSVISG 128
            H D              H R++RD  RV    +++S      + S+     F S ++SG
Sbjct: 66  LHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSG 125

Query: 129 LAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKSCYSQTDPVFNPAKSGSFAK 188
           + QGSGEYF RIGVG+PP+  YMV+D+GSD+VW+QC PCK CY Q+DPVF+PAKSGS+  
Sbjct: 126 MDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTG 185

Query: 189 VRCRTPLCSRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVDRVALGCGHD 248
           V C + +C R+E+ GC+    C Y+V YGDGSYT G    ETLTF +T V  VA+GCGH 
Sbjct: 186 VSCGSSVCDRIENSGCHS-GGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHR 245

Query: 249 NEGLFVGAAGLLGLGRGGLSFPSQTGRSFNQKFSYCLVDRSASSKPSSVVFGDSAVSRTA 308
           N G+F+GAAGLLG+G G +SF  Q        F YCLV R   S   S+VFG  A+   A
Sbjct: 246 NRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREALPVGA 305

Query: 309 RFTPLLSNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVTRLNR 368
            + PL+ NPR  +FYYV L G+ VGG  +  +    F L  TG+GGV++D GT+VTRL  
Sbjct: 306 SWVPLVRNPRAPSFYYVGLKGLGVGGVRIP-LPDGVFDLTETGDGGVVMDTGTAVTRLPT 365

Query: 369 PAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHF-RGADVSLPASNY 428
            AY+A RD F++  ++L  A   S+FDTCYDLSG  +V+VPTV  +F  G  ++LPA N+
Sbjct: 366 AAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNF 425

Query: 429 LIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 469
           L+PVD SG +CFAFA + +GLSIIGNIQQ+G +V +D A   VGF P  C
Sbjct: 426 LMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of MC05g0434 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 395.2 bits (1014), Expect = 7.3e-110
Identity = 237/506 (46.84%), Postives = 304/506 (60.08%), Query Frame = 0


Query: 8   AFPFIFSLLALLSLS-------------------------AALSDFQTLVP-RPLPTSPA 67
           AFP   SLLA+++LS                         ++L   QT++   P  +S  
Sbjct: 2   AFPRFLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLT 61

Query: 68  LSWPESDADSNSFSESETGFTLQLHHLDAL--SLNSTPEELFHTRLQRDAIRVQKL---- 127
            + PES +D   F  S +  +L+LH  D    S +   + L  +RL+RD+ RV  +    
Sbjct: 62  TTKPESLSDP-VFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKI 121

Query: 128 ---------SSLGGARNL-SQVSGTGFSSSVISGLAQGSGEYFTRIGVGTPPKYVYMVLD 187
                    S L    N  ++      ++ V+SG +QGSGEYF+RIGVGTP K +Y+VLD
Sbjct: 122 RFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLD 181

Query: 188 TGSDIVWLQCAPCKSCYSQTDPVFNPAKSGSFAKVRCRTPLCSRLESPGCNQRQTCLYQV 247
           TGSD+ W+QC PC  CY Q+DPVFNP  S ++  + C  P CS LE+  C   + CLYQV
Sbjct: 182 TGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNK-CLYQV 241

Query: 248 SYGDGSYTTGEFVTETLTFRRT-KVDRVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQT 307
           SYGDGS+T GE  T+T+TF  + K++ VALGCGHDNEGLF GAAGLLGLG G LS  +Q 
Sbjct: 242 SYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM 301

Query: 308 GRSFNQKFSYCLVDRSASSKPSSVVFGDSAVSRTARFTPLLSNPRLDTFYYVELLGISVG 367
             +    FSYCLVDR  S K SS+ F    +       PLL N ++DTFYYV L G SVG
Sbjct: 302 KAT---SFSYCLVDRD-SGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVG 361

Query: 368 GTPVSGISASHFKLDPTGNGGVIIDCGTSVTRLNRPAYIALRDAF-RAGASSLKSAPEFS 427
           G  V  +  + F +D +G+GGVI+DCGT+VTRL   AY +LRDAF +   +  K +   S
Sbjct: 362 GEKVV-LPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSIS 421

Query: 428 LFDTCYDLSGKTTVKVPTVVLHFRGA-DVSLPASNYLIPVDGSGRFCFAFAGTTSGLSII 469
           LFDTCYD S  +TVKVPTV  HF G   + LPA NYLIPVD SG FCFAFA T+S LSII
Sbjct: 422 LFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSII 481

The following BLAST results are available for this feature:

BLAST of MC05g0434 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LNJ3	1.3e-183	69.85	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	4.6e-109	47.02	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LS40	1.0e-108	46.84	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LEW3	5.7e-75	39.95	Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1	[more]
Q8S9J6	1.2e-72	40.30	Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At...	[more]

BLAST of MC05g0434 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022945440.1	1.38e-301	89.24	aspartyl protease family protein 2-like [Cucurbita moschata]	[more]
XP_023538763.1	1.38e-301	89.45	aspartyl protease family protein 2-like [Cucurbita pepo subsp. pepo]	[more]
KAG6597078.1	7.99e-301	89.24	Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022974939.1	6.31e-300	89.03	aspartyl protease family protein 2-like [Cucurbita maxima]	[more]
XP_022975090.1	6.55e-300	88.82	aspartyl protease family protein 2-like [Cucurbita maxima]	[more]

BLAST of MC05g0434 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G0Y1	6.70e-302	89.24	aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1IJ02	3.06e-300	89.03	aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111473...	[more]
A0A6J1IFR8	3.17e-300	88.82	aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111474...	[more]
A0A6J1IY26	9.74e-297	88.16	aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479...	[more]
A0A6J1EFG4	1.18e-295	87.76	aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of MC05g0434 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G01300.1	9.1e-185	69.85	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	3.1e-169	65.12	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	2.7e-112	51.64	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	3.3e-110	47.02	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	7.3e-110	46.84	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 346..357 score: 39.97 coord: 440..455 score: 26.83 coord: 133..153 score: 40.7
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 21..468
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 127..292 e-value: 2.0E-55 score: 187.8
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 290..469 e-value: 1.6E-53 score: 183.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 108..289 e-value: 3.2E-55 score: 189.1
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 120..468
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 313..464 e-value: 2.3E-35 score: 121.8
None	No IPR available	PANTHER	PTHR13683:SF761	ASPARTYL PROTEASE FAMILY PROTEIN 2-LIKE	coord: 21..468
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 142..153
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 127..464 score: 47.176857
IPR033873	CND41-like	CDD	cd05472	cnd41_like	coord: 126..468 e-value: 1.88281E-138 score: 398.183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0434.1	MC05g0434.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity