MC05g0424 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0424 vs. ExPASy Swiss-Prot
Match: O65717 (Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 PE=1 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.7e-200
Identity = 376/677 (55.54%), Postives = 484/677 (71.49%), Query Frame = 0

Query: 38  ASILESIQRKISSAS---EGFRRMVANLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNK 97
           +SI +   R   S+S   + F+R   +  FK   +   K      KILDPQ PFLQ+WNK
Sbjct: 41  SSISDKFYRSFESSSARIKLFKRSYKSYSFK---EAVSKGIGSTHKILDPQGPFLQRWNK 100

Query: 98  IFVLSCVLAVAVDPLFFYIPVMDRQHQCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRT 157
           IFVL+C++AV++DPLFFY+P++D   +CL +D+ + I A VLRSF D+FY+LHIIF+FRT
Sbjct: 101 IFVLACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRT 160

Query: 158 GFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLK 217
           GF+ PS  VFG G LVED   IAKRYLSS+FIIDIL+++PLPQ++ L+IIP  +G   L 
Sbjct: 161 GFIAPSSRVFGRGVLVEDKREIAKRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSLN 220

Query: 218 TKNFMKVAVLLQYLPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVFGAIW 277
           TKN +K  V  QY+PR  RIYPLY+EVTRTSGILTETAW+GAAFNL +YMLASHVFGA W
Sbjct: 221 TKNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGAFW 280

Query: 278 YLFSVEQESRCWHEACRKNN--CNSKYLYCGDHSRPAYGFIKEYCPHKESEDDKTFNFGI 337
           YLFS+E+E+ CW +AC +NN  C SK LYC   +     F+ E CP  ++ +   F+FGI
Sbjct: 281 YLFSIERETVCWKQACERNNPPCISKLLYCDPETAGGNAFLNESCP-IQTPNTTLFDFGI 340

Query: 338 YLEALKFRLTDQTTNFHSKFFYCFWWALRNVGSSGQNLQISNYLGELFFAVFIAILGLVL 397
           +L+AL+  +  ++ +F  KFFYCFWW L+N+ S GQNL+ S Y+ E+ FAVFI+I GLVL
Sbjct: 341 FLDALQSGVV-ESQDFPQKFFYCFWWGLQNLSSLGQNLKTSTYIWEICFAVFISIAGLVL 400

Query: 398 FALLISNIQKYLQSTTVKVEQMRINRRDAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGV 457
           F+ LI N+Q YLQSTT ++E+MR+ RRDAE WM HR+LPE+LR+RIRRY+QYKWQ  RGV
Sbjct: 401 FSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQETRGV 460

Query: 458 NEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQ 517
           +EE L+SNLPKDLRRDIKRHLCLALL +VP+F  MD+QLLDA+C+ L+PVL+TE+S I++
Sbjct: 461 DEENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMDEQLLDALCDRLQPVLYTEESYIVR 520

Query: 518 EGDPVDMMLFIMKGNLATIITYGWKND-SHSDVLKAGDFCGEELVPWAMDPQSTS-LPIS 577
           EGDPVD MLFIM+G L TI T G +    +S+ L AGDFCGEEL+ WA+DP S+S LPIS
Sbjct: 521 EGDPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLGAGDFCGEELLTWALDPHSSSNLPIS 580

Query: 578 TRTVKTLTEVEAFALKAKELQSVVSLF--------------YSHQWKVWAAYKIQEAWHD 637
           TRTV+ L EVEAFALKA +L+ V S F              YS QWK WAA  IQ AW  
Sbjct: 581 TRTVRALMEVEAFALKADDLKFVASQFRRLHSKQLRHTFRYYSQQWKTWAACFIQAAWRR 640

Query: 638 YCERKRRGG---GDSRFQGALAK-TVGASASFGATLYASIFISHLLQVVQRDQHQQTSQL 690
           Y ++K        ++R Q ALAK   G+S S GAT+YAS F +++L+ ++R    +  ++
Sbjct: 641 YIKKKLEESLKEEENRLQDALAKEACGSSPSLGATIYASRFAANILRTIRRSGSVRKPRM 700

BLAST of MC05g0424 vs. ExPASy Swiss-Prot
Match: Q9LNJ0 (Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 GN=CNGC10 PE=2 SV=2)

HSP 1 Score: 677.9 bits (1748), Expect = 1.2e-193
Identity = 363/680 (53.38%), Postives = 470/680 (69.12%), Query Frame = 0

Query: 43  SIQRKISSASEGFRRMVANLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVL 102
           S+ R   +   GF++ ++    K + D         K I++PQ  FLQ WNKIF+ +CV+
Sbjct: 31  SLDRVFKNVKWGFKKPLSFPSHK-DPDHKETSSVTRKNIINPQDSFLQNWNKIFLFACVV 90

Query: 103 AVAVDPLFFYIPVMDRQHQCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLP 162
           A+A+DPLFFYIP++D    CLT+D  L I A +LR+ ID FY++HI+F+FRT ++ PS  
Sbjct: 91  ALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRTLIDAFYIIHIVFQFRTAYIAPSSR 150

Query: 163 VFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVA 222
           VFG GELV+D  AIA +YLSS FIID+LSI+PLPQ++ L +IP+   P+ L TK+++K +
Sbjct: 151 VFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQIVVLAVIPSVNQPVSLLTKDYLKFS 210

Query: 223 VLLQYLPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQE 282
           ++ QY+PR+ R+YPLY EVTRTSGI+TETAW+GAA+NL +YMLASHVFGA+WYL SVE+E
Sbjct: 211 IIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAWNLSLYMLASHVFGALWYLISVERE 270

Query: 283 SRCWHEACRK-NNCNSKYLYCGDHSRPAYGFIKEYCPHKESED---DKTFNFGIYLEALK 342
            RCW EAC K   CN K+LYC +    +  F+   CP  +  D      FNFGI+ +ALK
Sbjct: 271 DRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSCPFLDPGDITNSTIFNFGIFTDALK 330

Query: 343 FRLTDQTTNFHSKFFYCFWWALRNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLIS 402
             +  ++ +F  KFFYCFWW LRN+ + GQNLQ S ++GE+ FA+ I I GLVLFALLI 
Sbjct: 331 SGVV-ESHDFWKKFFYCFWWGLRNLSALGQNLQTSKFVGEIIFAISICISGLVLFALLIG 390

Query: 403 NIQKYLQSTTVKVEQMRINRRDAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELI 462
           N+QKYL+STTV+ E+MR+ +RDAE WM HRMLPEDLR+RIRRY+QY+WQ  RGV EE L+
Sbjct: 391 NMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRRYEQYRWQETRGVEEETLL 450

Query: 463 SNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVD 522
            NLPKDLRRDIKRHLCL LLKKVPLF  MD+QLLDA+C+ L+PVL+TE S +++EGDPV 
Sbjct: 451 RNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDAVCDRLRPVLYTENSYVIREGDPVG 510

Query: 523 MMLFIMKGNLATIITYGWKNDSHSDV-LKAGDFCGEELVPWAMDPQSTS-LPISTRTVKT 582
            MLF+M+G L +  T G ++   + V LKA DFCGE+L+PWA+DPQS+S  PISTRTV+ 
Sbjct: 511 EMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGEDLLPWALDPQSSSHFPISTRTVQA 570

Query: 583 LTEVEAFALKAKELQSVVS--------------LFYSHQWKVWAAYKIQEAWHDYCERKR 642
           LTEVEAFAL A++L+SV S               FYS QW+ W+   IQ AW  YC RK 
Sbjct: 571 LTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFYSVQWRTWSVSFIQAAWRRYCRRKL 630

Query: 643 RGG---GDSRFQGALAK--------TVGASASFGATLYASIFISHLLQVVQRDQHQQTSQ 691
                  + R + ALA         TV +S S G  LYAS F S+ L  ++ +     S 
Sbjct: 631 AKSLRDEEDRLREALASQDKEHNAATVSSSLSLGGALYASRFASNALHNLRHN----ISN 690

BLAST of MC05g0424 vs. ExPASy Swiss-Prot
Match: Q9LD40 (Putative cyclic nucleotide-gated ion channel 13 OS=Arabidopsis thaliana OX=3702 GN=CNGC13 PE=3 SV=2)

HSP 1 Score: 667.5 bits (1721), Expect = 1.6e-190
Identity = 361/693 (52.09%), Postives = 472/693 (68.11%), Query Frame = 0

Query: 26  YGGYQNRSRLSPASILESIQRKISSASEGFRRMVANLGFKHEDDQSRKKPEFWKKILDPQ 85
           YG  +N++R S  ++L++++R +        +   + G  ++   S       K I++PQ
Sbjct: 21  YGYGRNKARPSLNTVLKNVRRGL--------KKPLSFGSHNKKRDSNSSTTTQKNIINPQ 80

Query: 86  KPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQHQCLTMDRLLMIIACVLRSFIDIFYL 145
             FLQ WNKIF+ + V+A+A+DPLFFYIP++D +  CL + R L I A VLR+FID FY+
Sbjct: 81  GSFLQNWNKIFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRTFIDAFYI 140

Query: 146 LHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQLLALVIIP 205
           +HI+F+FRT ++ PS  VFG GELV+DP AIA +YLSS FIID+LSI+PLPQL+ L +IP
Sbjct: 141 IHIVFQFRTAYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQLVVLAVIP 200

Query: 206 AAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYML 265
               P+ L TK+++   +  QY+PR+ RIYPLY EVTRTSGI+TETAW+GAA+NL +YML
Sbjct: 201 NVNKPVSLITKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAWNLSLYML 260

Query: 266 ASHVFGAIWYLFSVEQESRCWHEACRK--NNCNSKYLYCGDHSRPAYGFIKEYCPHKESE 325
           ASHVFGA+WYL SVE+E RCW EAC K    CN ++LYC  +S     F+   CP    +
Sbjct: 261 ASHVFGALWYLISVEREDRCWREACEKIPEVCNFRFLYCDGNSSVRNDFLTTSCPFINPD 320

Query: 326 D---DKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVGSSGQNLQISNYLGELF 385
           D      FNFGI+ +ALK  +  ++ +F  KFFYCFWW LRN+ + GQNL  S ++GE+ 
Sbjct: 321 DITNSTVFNFGIFTDALKSGIV-ESDDFWKKFFYCFWWGLRNLSALGQNLNTSKFVGEII 380

Query: 386 FAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRDAEHWMVHRMLPEDLRQRIRR 445
           FAV I I GLVLFALLI N+QKYL+STTV+ E+MR+ +RDAE WM HRMLP+DLR+RIRR
Sbjct: 381 FAVSICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPDDLRKRIRR 440

Query: 446 YDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAMCEYLK 505
           Y+QYKWQ  RGV EE L+ NLPKDLRRDIKRH CL LLKKVPLF  MD+QLLDA+C+ LK
Sbjct: 441 YEQYKWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLLKKVPLFEIMDEQLLDAVCDKLK 500

Query: 506 PVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDSHSDV-LKAGDFCGEELVPWA 565
           PVL+TE S  ++EGDPV+ MLF+M+G L +  T G +    + V LK  DFCGE+L+ WA
Sbjct: 501 PVLYTENSYAIREGDPVEEMLFVMRGKLMSATTNGGRTGFFNAVYLKPSDFCGEDLLTWA 560

Query: 566 MDPQSTS-LPISTRTVKTLTEVEAFALKAKELQSVVS--------------LFYSHQWKV 625
           +DPQS+S  PISTRTV+ LTEVEAFAL A +L+ V S               FYS QW+ 
Sbjct: 561 LDPQSSSHFPISTRTVQALTEVEAFALAADDLKLVASQFRRLHSKQLQHTFRFYSVQWRT 620

Query: 626 WAAYKIQEAWHDYCERKRRGG---GDSRFQGALAK---TVGASASFGATLYASIFISHLL 685
           W A  IQ AW  +C RK        + RF+ A+ K      +S+S  ATLYAS F S+ L
Sbjct: 621 WGASFIQAAWRRHCRRKLARSLTEEEDRFRNAITKRERNAASSSSLVATLYASRFASNAL 680

Query: 686 QVVQRDQHQQTSQLSRVMTLPPPPKPIERDFTI 692
                 ++ +T+ L  +     PPKP E DF++
Sbjct: 681 ------RNLRTNNLPLL-----PPKPSEPDFSL 693

BLAST of MC05g0424 vs. ExPASy Swiss-Prot
Match: Q9SKD7 (Probable cyclic nucleotide-gated ion channel 3 OS=Arabidopsis thaliana OX=3702 GN=CNGC3 PE=2 SV=2)

HSP 1 Score: 630.2 bits (1624), Expect = 2.8e-179
Identity = 340/702 (48.43%), Postives = 460/702 (65.53%), Query Frame = 0

Query: 18  PTSDIHVRYGGYQNR-----SRLSPASILESIQRKISSASEGFRRMVANLGFKHEDDQSR 77
           P  +  VR+ G  +      +R S +S++++++R     SE  R     L      ++  
Sbjct: 4   PQRNKFVRFNGNDDEFSTKTTRPSVSSVMKTVRRSFEKGSEKIRTFKRPLSVHSNKNKEN 63

Query: 78  KKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQHQCLTMDRLLMII 137
            K +   ++++P   +LQ WNKIF+L  V+A+A DPLFFYIP +  +  CL +D+ L  I
Sbjct: 64  NKKKKILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPERFCLNLDKKLQTI 123

Query: 138 ACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSI 197
           ACV R+FID FY++H++F+F TGF+ PS   FG GEL E    IA RYL S F+ID+LSI
Sbjct: 124 ACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALRYLGSYFLIDLLSI 183

Query: 198 IPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYREVTRTSGILTETA 257
           +P+PQ++ L I+P  + P  L  K  +K  +  QY+PR+ RIYPL++EVTRTSG++TETA
Sbjct: 184 LPIPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKEVTRTSGLVTETA 243

Query: 258 WSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRK-NNCNSKYLYCGDHSRPAYG 317
           W+GAA NL +YMLASHVFG+ WYL S+E++ RCW EAC K  NC   YLYC         
Sbjct: 244 WAGAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAYLYCSPTGEDNRL 303

Query: 318 FIKEYCP---HKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVGSSGQ 377
           F+   CP    +E  +   FNFGI+ +AL+  +  ++ +F  KFFYCFWW LRN+ + GQ
Sbjct: 304 FLNGSCPLIDPEEITNSTVFNFGIFADALQSGVV-ESRDFPKKFFYCFWWGLRNLSALGQ 363

Query: 378 NLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRDAEHWMVHR 437
           NL+ S + GE+ FA+ I I GLVLFALLI N+QKYLQSTTV+VE+MR+ RRDAE WM HR
Sbjct: 364 NLKTSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMRVKRRDAEQWMSHR 423

Query: 438 MLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMD 497
           MLP+DLR+RIR+Y+QYKWQ  +GV EE L+S+LPKDLR+DIKRHLCL LLKKVP F +MD
Sbjct: 424 MLPDDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLCLKLLKKVPWFQAMD 483

Query: 498 KQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDSHSDV-LKA 557
            +LLDA+C  LK VL+TEKS I++EG+PV+ MLFIM+GNL +  TYG +    + V L A
Sbjct: 484 DRLLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYGGRTGFFNSVDLVA 543

Query: 558 GDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVS------------ 617
           GDFCG +L+ WA+DP S+  PIS+RTV+ LTEVE F L A +L+ V +            
Sbjct: 544 GDFCG-DLLTWALDPLSSQFPISSRTVQALTEVEGFLLSADDLKFVATQYRRLHSKQLRH 603

Query: 618 --LFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTV------GASASFGAT 677
              FYS QW+ WAA  IQ AW  +C RK         +G L  T+      G   + GA 
Sbjct: 604 MFRFYSVQWQTWAACFIQAAWKRHCRRKLSKALREE-EGKLHNTLQNDDSGGNKLNLGAA 663

Query: 678 LYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIERDF 690
           +YAS F SH L+ ++ +   + S+   ++TL  P KP + +F
Sbjct: 664 IYASRFASHALRNLRANAAARNSRFPHMLTL-LPQKPADPEF 701

BLAST of MC05g0424 vs. ExPASy Swiss-Prot
Match: Q8RWS9 (Probable cyclic nucleotide-gated ion channel 5 OS=Arabidopsis thaliana OX=3702 GN=CNGC5 PE=2 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 2.2e-171
Identity = 323/676 (47.78%), Postives = 444/676 (65.68%), Query Frame = 0

Query: 43  SIQRKISSASEGFRRMVANLGF---KHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLS 102
           S ++ I   S+G + +  +LGF   +    +  K  E  KKI DPQ  FL   NK+FV S
Sbjct: 49  SFKKGIQKGSKGLKSIGRSLGFGVYRAVFPEDLKVSE--KKIFDPQDKFLLYCNKLFVAS 108

Query: 103 CVLAVAVDPLFFYIPVMDRQHQCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPP 162
           C+L+V VDP FFY+PV++ + +CL +DR L I A  LR+FID+FYL H+  + RT ++ P
Sbjct: 109 CILSVFVDPFFFYLPVINAESKCLGIDRKLAITASTLRTFIDVFYLAHMALQLRTAYIAP 168

Query: 163 SLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFM 222
           S  VFG GELV DPA IAKRYL   FIID LS++PLPQ++    + ++ G   L TK  +
Sbjct: 169 SSRVFGRGELVIDPAQIAKRYLQRWFIIDFLSVLPLPQIVVWRFLQSSNGSDVLATKQAL 228

Query: 223 KVAVLLQYLPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSV 282
              VL+QY+PR  R+ PL  E+ RT+G+  ETAW+GAA+ LL+YMLASH+ GA WYL ++
Sbjct: 229 LFIVLVQYIPRFLRVLPLTSELKRTAGVFAETAWAGAAYYLLLYMLASHIVGAFWYLLAL 288

Query: 283 EQESRCWHEAC-RKNNCNSKYLYCGDHSRPAY--------GFIKEYCPHKESEDDKTFNF 342
           E+   CW EAC    NC++ +LYCG+ +   Y          +K  C     +++  F+F
Sbjct: 289 ERNDACWQEACIDAGNCSTDFLYCGNQNMDGYAVWNRAKESVLKSKCRADLDDNNPPFDF 348

Query: 343 GIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVGSSGQNLQISNYLGELFFAVFIAILGL 402
           GIY +AL   +   + NF  K+ YC WW L+N+ + GQ L+ S Y  E+ F++ +AI GL
Sbjct: 349 GIYTQALSSGIV-SSQNFIVKYCYCLWWGLQNLSTLGQGLETSTYPMEIIFSISLAISGL 408

Query: 403 VLFALLISNIQKYLQSTTVKVEQMRINRRDAEHWMVHRMLPEDLRQRIRRYDQYKWQLNR 462
           +LFALLI N+Q YLQS T+++E+MR+ RRD+E WM HRMLP+DLR+R+RRYDQYKW   R
Sbjct: 409 ILFALLIGNMQTYLQSLTIRLEEMRVKRRDSEQWMHHRMLPQDLRERVRRYDQYKWLETR 468

Query: 463 GVNEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSDI 522
           GV+EE L+ NLPKDLRRDIKRHLCLAL+++VPLF SMD +LLDA+C  LKP LFTE + +
Sbjct: 469 GVDEEYLVQNLPKDLRRDIKRHLCLALVRRVPLFKSMDDKLLDAICMRLKPCLFTESTYL 528

Query: 523 LQEGDPVDMMLFIMKGNLATIITYGWKND-SHSDVLKAGDFCGEELVPWAMDPQS-TSLP 582
           ++EGDPVD MLFI++G L ++ T G ++   +  +LK G+FCGEEL+ WA+DP+S  +LP
Sbjct: 529 VREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRSLLKEGEFCGEELLTWALDPKSGVNLP 588

Query: 583 ISTRTVKTLTEVEAFALKAKELQSVVS--------------LFYSHQWKVWAAYKIQEAW 642
            STRTVK LTEVEAFAL ++EL+ V S               FYSHQW+ WAA  IQ AW
Sbjct: 589 SSTRTVKALTEVEAFALTSEELKFVASQFRRLHSRQVQHTFRFYSHQWRTWAACFIQAAW 648

Query: 643 HDYCERKRRGGGDSRFQGALAKTVGASASFGATLYASIFISHLLQVVQRDQHQQTSQLSR 691
             YC+RK+    ++      + T G S S GA   A+ F ++ L+ + R+++ +   L +
Sbjct: 649 RRYCKRKKMEEAEAEAAAVSSSTAGPSYSIGAAFLATKFAANALRTIHRNRNTKIRDLVK 708

BLAST of MC05g0424 vs. NCBI nr
Match: XP_022147438.1 (cyclic nucleotide-gated ion channel 1-like [Momordica charantia])

HSP 1 Score: 1375 bits (3559), Expect = 0.0
Identity = 693/710 (97.61%), Postives = 693/710 (97.61%), Query Frame = 0

Query: 1   MSKFMGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVA 60
           MSKFMGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVA
Sbjct: 1   MSKFMGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVA 60

Query: 61  NLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQH 120
           NLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQH
Sbjct: 61  NLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQH 120

Query: 121 QCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 180
           QCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY
Sbjct: 121 QCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 180

Query: 181 LSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYRE 240
           LSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYRE
Sbjct: 181 LSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYRE 240

Query: 241 VTRTSGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRKNNCNSKYL 300
           VTRTSGILTETAWSGAAFNLLIYMLASHV GAIWYLFSVEQESRCWHEACRKNNCNSKYL
Sbjct: 241 VTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSVEQESRCWHEACRKNNCNSKYL 300

Query: 301 YCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWAL 360
           YCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWAL
Sbjct: 301 YCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWAL 360

Query: 361 RNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRD 420
           RNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRD
Sbjct: 361 RNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRD 420

Query: 421 AEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKK 480
           AEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKK
Sbjct: 421 AEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKK 480

Query: 481 VPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDS 540
           VPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDS
Sbjct: 481 VPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDS 540

Query: 541 HSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF--- 600
           HSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF   
Sbjct: 541 HSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLFRFQ 600

Query: 601 -------------YSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTVGASASF 660
                        YSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTVGASASF
Sbjct: 601 RFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTVGASASF 660

Query: 661 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIERDFTILDL 694
           GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIERDFTILDL
Sbjct: 661 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIERDFTILDL 710

BLAST of MC05g0424 vs. NCBI nr
Match: XP_022147437.1 (LOW QUALITY PROTEIN: cyclic nucleotide-gated ion channel 1-like [Momordica charantia])

HSP 1 Score: 1236 bits (3199), Expect = 0.0
Identity = 634/713 (88.92%), Postives = 651/713 (91.30%), Query Frame = 0

Query: 1   MSKFMGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVA 60
           MSKFMGSLKPK  RFGYPTSDIHV+YGGYQNRS LSPASILESIQR+ SSASEGFRRM A
Sbjct: 3   MSKFMGSLKPKFFRFGYPTSDIHVKYGGYQNRSTLSPASILESIQRRFSSASEGFRRMEA 62

Query: 61  NLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQH 120
            L FKHE DQ RKK E  KKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPV+DRQH
Sbjct: 63  VLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQH 122

Query: 121 QCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 180
           QCLTMDRLLMIIAC+LRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY
Sbjct: 123 QCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 182

Query: 181 LSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYRE 240
           LSSNFIIDILSIIPLPQLL LVIIPAAKGPI L+TK+F+KVAVLLQY+PRL RIY LYRE
Sbjct: 183 LSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYRE 242

Query: 241 VTRTSGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRKNNCNSKYL 300
           VTRTSGILTE AWSGAAFNLLIYMLASHV GAIWYLFSVEQE RCW EACR  NCNSK L
Sbjct: 243 VTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNSKDL 302

Query: 301 YCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWAL 360
            CGD SR AY  IK+YC HK+SEDDKTFNFG+YLEALKF LTDQTTNF  KFFYCFWWAL
Sbjct: 303 CCGDPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWAL 362

Query: 361 RNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRD 420
           RNVGS GQNLQISNYLGE+ FAVFIAILGL+LFALLISNIQKYLQSTTVKVEQMRI+RRD
Sbjct: 363 RNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRD 422

Query: 421 AEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKK 480
           AEHWM HRMLPEDLRQRIRRYDQYKWQLNRGV EEELISNLPKDLRRDI RHLCLALLKK
Sbjct: 423 AEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKK 482

Query: 481 VPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDS 540
           VPLFSSMDKQLLDAMCEYLKPVLFTE S+ILQEGDP+DMMLFIMKGNLATIITYGWKNDS
Sbjct: 483 VPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDS 542

Query: 541 HSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF--- 600
           HSDVLKAGDFCGEELVPWAMDPQSTSLPI TRTVKTLTEVEAFALKAKELQSVVS F   
Sbjct: 543 HSDVLKAGDFCGEELVPWAMDPQSTSLPIXTRTVKTLTEVEAFALKAKELQSVVSQFHFQ 602

Query: 601 -------------YSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTVGASASF 660
                        YSHQWKVWAAYKIQEAWHDYCERKRRGGGD RFQ ALAKT GASASF
Sbjct: 603 RFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGASASF 662

Query: 661 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIE---RDFTILDL 694
           GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKP+E   RDFTILDL
Sbjct: 663 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL 714

BLAST of MC05g0424 vs. NCBI nr
Match: XP_022924449.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >XP_022924450.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >KAG6582669.1 Cyclic nucleotide-gated ion channel 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1090 bits (2820), Expect = 0.0
Identity = 552/710 (77.75%), Postives = 609/710 (85.77%), Query Frame = 0

Query: 5   MGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVANLGF 64
           MGS  PKS RFG+PTSD H +YG YQN SRLSP SILESI+RK  SA + FRR++ +L F
Sbjct: 1   MGSQIPKSTRFGHPTSDFHDKYGSYQNGSRLSPGSILESIERKFLSAYKSFRRIITHLSF 60

Query: 65  KHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQHQCLT 124
           KH  DQS KKP+FWKKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPV++ + QCLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQCLT 120

Query: 125 MDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSN 184
           +DR LMIIACVLRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLFSN 180

Query: 185 FIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYREVTRT 244
           F+IDILS++PLPQLL L IIPAA+GP+PLKTK+ +K+AVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAARGPVPLKTKDLVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRKNNCNSKYLYCGD 304
            GILTETAWSGAAFNLLIYM  SHV GAIWYL SVE++++CW EACRK+ C   +LYCG+
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDNFLYCGN 300

Query: 305 HSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVG 364
             R    +I +YC  KESED KTFNFG+Y+EALKF+LT  TT+F  KFFY FWWALRNV 
Sbjct: 301 QRRQIQPYIDQYCSPKESEDGKTFNFGMYVEALKFQLT-STTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRDAEHW 424
           SSGQNLQ+SNY+GE+FFAVFIAILGLVLFALLISNIQKYLQS TVKVEQMRINRRDAEHW
Sbjct: 361 SSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           M HRMLPEDLRQRIRRYDQYKWQLNRGV EEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDSHSDV 544
           S MDKQLLDAMCEYLKPVLFTEKS ILQEGDP+DM+LFIMKG LATIITYGWKND+ S V
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMVLFIMKGKLATIITYGWKNDTLSGV 540

Query: 545 LKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF------- 604
           L AGDFCGEELV WAMDP STSLPIS+RTVK+LTEVEAFALKA EL+SV S F       
Sbjct: 541 LNAGDFCGEELVQWAMDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQHLNS 600

Query: 605 ---------YSHQWKVWAAYKIQEAWHDYCERKRRG-GGDSRFQGALAKTVGASASFGAT 664
                    YSHQW VWAAYK+QEAW DY ERKRRG  GD RFQ ALAKT GAS  FGAT
Sbjct: 601 KQFQLSVRFYSHQWNVWAAYKVQEAWQDYRERKRRGVRGDGRFQDALAKTAGASVGFGAT 660

Query: 665 LYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIER----DFTILD 693
           LYASIFISHLLQVVQRDQHQ+T+QL++VMTLPPPPKP +     +FTIL+
Sbjct: 661 LYASIFISHLLQVVQRDQHQETTQLTQVMTLPPPPKPDDEPDSPNFTILN 709

BLAST of MC05g0424 vs. NCBI nr
Match: XP_022980030.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980031.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980033.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980034.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1088 bits (2815), Expect = 0.0
Identity = 554/711 (77.92%), Postives = 607/711 (85.37%), Query Frame = 0

Query: 5   MGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVANLGF 64
           MGS  PKS RFG+PTSD H +YG +QNRSRLSP SILESI+RK SSA +  RR++ +L F
Sbjct: 1   MGSQIPKSARFGHPTSDFHDKYGSHQNRSRLSPGSILESIERKFSSAYKSVRRIITHLSF 60

Query: 65  KHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQHQCLT 124
           KH  DQS KKP+FWKKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPV+D + QCLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVIDGERQCLT 120

Query: 125 MDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSN 184
           +DR LMIIACVLRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLLSN 180

Query: 185 FIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYREVTRT 244
           F+IDILS++PLPQLL L IIPAAKGPIPLKTK+ +K+AVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRKNNCNSKYLYCGD 304
            GILTETAWSGAAFNLLIYM  SHV GAIWYL SVE++++CW EACRK+ C   +LYCG+
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDNFLYCGN 300

Query: 305 HSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVG 364
             R    +I +YC  KE ED KTFNFG+Y+EALKF+LT  TT+F  KFFY FWWALRNV 
Sbjct: 301 QRRQIQPYIDQYCSPKEPEDGKTFNFGMYVEALKFQLT-STTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRDAEHW 424
           SSGQNLQ+SNY+GE+FFAVFIAILGLVLFALLISNIQKYLQS TVKVEQMRINRRDAEHW
Sbjct: 361 SSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           M HRMLPEDLRQRIRRYDQYKWQLNRGV EEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDSHSDV 544
           S MDKQLLDAMCEYLKPVLFTEKS ILQEGDP+DMMLFIMKG LATIITYGWKND+ S V
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIITYGWKNDTFSAV 540

Query: 545 LKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF------- 604
           L AGDFCGEELV WA DP STSLPIS+RTVK+LTEVEAFALKA EL+SV S F       
Sbjct: 541 LNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQRLNS 600

Query: 605 ---------YSHQWKVWAAYKIQEAWHDYCERKRRG-GGDSRFQGALAKTVGASASFGAT 664
                    YSHQW VWAAYKIQEAW +Y ERKRRG  GD RFQ ALAKT GAS  FGAT
Sbjct: 601 KQFQLSVRFYSHQWNVWAAYKIQEAWQEYRERKRRGVRGDGRFQAALAKTAGASVGFGAT 660

Query: 665 LYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIER----DFTILDL 694
           LYASIFISHLLQVVQRDQH++T QL+RVMTLPPPPKP +     + TIL+L
Sbjct: 661 LYASIFISHLLQVVQRDQHRETVQLTRVMTLPPPPKPDDEPDRPNVTILNL 710

BLAST of MC05g0424 vs. NCBI nr
Match: XP_023526877.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526878.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526879.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1071 bits (2769), Expect = 0.0
Identity = 546/710 (76.90%), Postives = 603/710 (84.93%), Query Frame = 0

Query: 5   MGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVANLGF 64
           MGS  PKS RFG+PTSD H +YG YQN SRLSP SILESI+RK SSA +  RR++ +  F
Sbjct: 1   MGSQIPKSTRFGHPTSDFHDKYGSYQNGSRLSPGSILESIERKFSSAYKSSRRIITHFSF 60

Query: 65  KHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQHQCLT 124
           KH  DQS KKP+F KKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPV++ + QCLT
Sbjct: 61  KHPGDQSVKKPKFRKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQCLT 120

Query: 125 MDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSN 184
           +DR LMIIACVLRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLFSN 180

Query: 185 FIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYREVTRT 244
           F+IDILS++PLPQLL L IIPAAKGPIPLKTK+ +K+AVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRKNNCNSKYLYCGD 304
            GILTETAWSGAAFNLLIYM  SHV GAIWYL SVE++++CW EACRK  C   +LYCG+
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKGKCKDNFLYCGN 300

Query: 305 HSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVG 364
             R  + +I +YC  KESED+KTF FG+++EALKF+LT  TT+F  KFFY FWWALRNV 
Sbjct: 301 QRRQIHPYIDQYCSPKESEDEKTFKFGMFVEALKFQLT-STTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRDAEHW 424
           SSGQNLQ+SNY+G++FFAVFIAILGLVLFALLISNIQKYLQS TVKVEQMRINRRDAEHW
Sbjct: 361 SSGQNLQVSNYMGDVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           M HRMLPEDLRQRIRRYDQYKWQLNRGV EEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDSHSDV 544
           S MDKQLLDAMCEYLKPVLFTEKS ILQEGDP+DMMLFIMKG LAT ITYGWKN++ S V
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATSITYGWKNNTFSGV 540

Query: 545 LKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF------- 604
           L AGDFCGEELV WA DP STSLPIS+RTVK+LTEVEAFALKA EL+SV S F       
Sbjct: 541 LNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQRLNS 600

Query: 605 ---------YSHQWKVWAAYKIQEAWHDYCERKRRG-GGDSRFQGALAKTVGASASFGAT 664
                    YSHQW VWAAYKIQEAW +Y ERKRRG  GD RFQ ALAK  GAS  FGAT
Sbjct: 601 KQFQLSVRFYSHQWNVWAAYKIQEAWQEYRERKRRGVRGDGRFQDALAKMAGASVGFGAT 660

Query: 665 LYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIER----DFTILD 693
           LYASIFISHLLQVVQRDQ Q+T+QL+RVMTLPPPPKP +     +FTIL+
Sbjct: 661 LYASIFISHLLQVVQRDQPQETAQLTRVMTLPPPPKPDDEPDSPNFTILN 709

BLAST of MC05g0424 vs. ExPASy TrEMBL
Match: A0A6J1D1B4 (cyclic nucleotide-gated ion channel 1-like OS=Momordica charantia OX=3673 GN=LOC111016363 PE=4 SV=1)

HSP 1 Score: 1375 bits (3559), Expect = 0.0
Identity = 693/710 (97.61%), Postives = 693/710 (97.61%), Query Frame = 0

Query: 1   MSKFMGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVA 60
           MSKFMGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVA
Sbjct: 1   MSKFMGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVA 60

Query: 61  NLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQH 120
           NLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQH
Sbjct: 61  NLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQH 120

Query: 121 QCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 180
           QCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY
Sbjct: 121 QCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 180

Query: 181 LSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYRE 240
           LSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYRE
Sbjct: 181 LSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYRE 240

Query: 241 VTRTSGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRKNNCNSKYL 300
           VTRTSGILTETAWSGAAFNLLIYMLASHV GAIWYLFSVEQESRCWHEACRKNNCNSKYL
Sbjct: 241 VTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSVEQESRCWHEACRKNNCNSKYL 300

Query: 301 YCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWAL 360
           YCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWAL
Sbjct: 301 YCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWAL 360

Query: 361 RNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRD 420
           RNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRD
Sbjct: 361 RNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRD 420

Query: 421 AEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKK 480
           AEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKK
Sbjct: 421 AEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKK 480

Query: 481 VPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDS 540
           VPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDS
Sbjct: 481 VPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDS 540

Query: 541 HSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF--- 600
           HSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF   
Sbjct: 541 HSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLFRFQ 600

Query: 601 -------------YSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTVGASASF 660
                        YSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTVGASASF
Sbjct: 601 RFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTVGASASF 660

Query: 661 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIERDFTILDL 694
           GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIERDFTILDL
Sbjct: 661 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIERDFTILDL 710

BLAST of MC05g0424 vs. ExPASy TrEMBL
Match: A0A6J1D2C8 (LOW QUALITY PROTEIN: cyclic nucleotide-gated ion channel 1-like OS=Momordica charantia OX=3673 GN=LOC111016362 PE=4 SV=1)

HSP 1 Score: 1236 bits (3199), Expect = 0.0
Identity = 634/713 (88.92%), Postives = 651/713 (91.30%), Query Frame = 0

Query: 1   MSKFMGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVA 60
           MSKFMGSLKPK  RFGYPTSDIHV+YGGYQNRS LSPASILESIQR+ SSASEGFRRM A
Sbjct: 3   MSKFMGSLKPKFFRFGYPTSDIHVKYGGYQNRSTLSPASILESIQRRFSSASEGFRRMEA 62

Query: 61  NLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQH 120
            L FKHE DQ RKK E  KKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPV+DRQH
Sbjct: 63  VLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVIDRQH 122

Query: 121 QCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 180
           QCLTMDRLLMIIAC+LRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY
Sbjct: 123 QCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 182

Query: 181 LSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYRE 240
           LSSNFIIDILSIIPLPQLL LVIIPAAKGPI L+TK+F+KVAVLLQY+PRL RIY LYRE
Sbjct: 183 LSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLLRIYLLYRE 242

Query: 241 VTRTSGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRKNNCNSKYL 300
           VTRTSGILTE AWSGAAFNLLIYMLASHV GAIWYLFSVEQE RCW EACR  NCNSK L
Sbjct: 243 VTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRTRNCNSKDL 302

Query: 301 YCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWAL 360
            CGD SR AY  IK+YC HK+SEDDKTFNFG+YLEALKF LTDQTTNF  KFFYCFWWAL
Sbjct: 303 CCGDPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKFFYCFWWAL 362

Query: 361 RNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRD 420
           RNVGS GQNLQISNYLGE+ FAVFIAILGL+LFALLISNIQKYLQSTTVKVEQMRI+RRD
Sbjct: 363 RNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVEQMRISRRD 422

Query: 421 AEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKK 480
           AEHWM HRMLPEDLRQRIRRYDQYKWQLNRGV EEELISNLPKDLRRDI RHLCLALLKK
Sbjct: 423 AEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRHLCLALLKK 482

Query: 481 VPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDS 540
           VPLFSSMDKQLLDAMCEYLKPVLFTE S+ILQEGDP+DMMLFIMKGNLATIITYGWKNDS
Sbjct: 483 VPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATIITYGWKNDS 542

Query: 541 HSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF--- 600
           HSDVLKAGDFCGEELVPWAMDPQSTSLPI TRTVKTLTEVEAFALKAKELQSVVS F   
Sbjct: 543 HSDVLKAGDFCGEELVPWAMDPQSTSLPIXTRTVKTLTEVEAFALKAKELQSVVSQFHFQ 602

Query: 601 -------------YSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTVGASASF 660
                        YSHQWKVWAAYKIQEAWHDYCERKRRGGGD RFQ ALAKT GASASF
Sbjct: 603 RFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDGRFQDALAKTAGASASF 662

Query: 661 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIE---RDFTILDL 694
           GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKP+E   RDFTILDL
Sbjct: 663 GATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDFTILDL 714

BLAST of MC05g0424 vs. ExPASy TrEMBL
Match: A0A6J1EF20 (cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431950 PE=4 SV=1)

HSP 1 Score: 1090 bits (2820), Expect = 0.0
Identity = 552/710 (77.75%), Postives = 609/710 (85.77%), Query Frame = 0

Query: 5   MGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVANLGF 64
           MGS  PKS RFG+PTSD H +YG YQN SRLSP SILESI+RK  SA + FRR++ +L F
Sbjct: 1   MGSQIPKSTRFGHPTSDFHDKYGSYQNGSRLSPGSILESIERKFLSAYKSFRRIITHLSF 60

Query: 65  KHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQHQCLT 124
           KH  DQS KKP+FWKKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPV++ + QCLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQCLT 120

Query: 125 MDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSN 184
           +DR LMIIACVLRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLFSN 180

Query: 185 FIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYREVTRT 244
           F+IDILS++PLPQLL L IIPAA+GP+PLKTK+ +K+AVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAARGPVPLKTKDLVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRKNNCNSKYLYCGD 304
            GILTETAWSGAAFNLLIYM  SHV GAIWYL SVE++++CW EACRK+ C   +LYCG+
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDNFLYCGN 300

Query: 305 HSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVG 364
             R    +I +YC  KESED KTFNFG+Y+EALKF+LT  TT+F  KFFY FWWALRNV 
Sbjct: 301 QRRQIQPYIDQYCSPKESEDGKTFNFGMYVEALKFQLT-STTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRDAEHW 424
           SSGQNLQ+SNY+GE+FFAVFIAILGLVLFALLISNIQKYLQS TVKVEQMRINRRDAEHW
Sbjct: 361 SSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           M HRMLPEDLRQRIRRYDQYKWQLNRGV EEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDSHSDV 544
           S MDKQLLDAMCEYLKPVLFTEKS ILQEGDP+DM+LFIMKG LATIITYGWKND+ S V
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMVLFIMKGKLATIITYGWKNDTLSGV 540

Query: 545 LKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF------- 604
           L AGDFCGEELV WAMDP STSLPIS+RTVK+LTEVEAFALKA EL+SV S F       
Sbjct: 541 LNAGDFCGEELVQWAMDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQHLNS 600

Query: 605 ---------YSHQWKVWAAYKIQEAWHDYCERKRRG-GGDSRFQGALAKTVGASASFGAT 664
                    YSHQW VWAAYK+QEAW DY ERKRRG  GD RFQ ALAKT GAS  FGAT
Sbjct: 601 KQFQLSVRFYSHQWNVWAAYKVQEAWQDYRERKRRGVRGDGRFQDALAKTAGASVGFGAT 660

Query: 665 LYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIER----DFTILD 693
           LYASIFISHLLQVVQRDQHQ+T+QL++VMTLPPPPKP +     +FTIL+
Sbjct: 661 LYASIFISHLLQVVQRDQHQETTQLTQVMTLPPPPKPDDEPDSPNFTILN 709

BLAST of MC05g0424 vs. ExPASy TrEMBL
Match: A0A6J1IV06 (cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479553 PE=4 SV=1)

HSP 1 Score: 1088 bits (2815), Expect = 0.0
Identity = 554/711 (77.92%), Postives = 607/711 (85.37%), Query Frame = 0

Query: 5   MGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVANLGF 64
           MGS  PKS RFG+PTSD H +YG +QNRSRLSP SILESI+RK SSA +  RR++ +L F
Sbjct: 1   MGSQIPKSARFGHPTSDFHDKYGSHQNRSRLSPGSILESIERKFSSAYKSVRRIITHLSF 60

Query: 65  KHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQHQCLT 124
           KH  DQS KKP+FWKKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPV+D + QCLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVIDGERQCLT 120

Query: 125 MDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSN 184
           +DR LMIIACVLRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLLSN 180

Query: 185 FIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYREVTRT 244
           F+IDILS++PLPQLL L IIPAAKGPIPLKTK+ +K+AVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRKNNCNSKYLYCGD 304
            GILTETAWSGAAFNLLIYM  SHV GAIWYL SVE++++CW EACRK+ C   +LYCG+
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDNFLYCGN 300

Query: 305 HSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVG 364
             R    +I +YC  KE ED KTFNFG+Y+EALKF+LT  TT+F  KFFY FWWALRNV 
Sbjct: 301 QRRQIQPYIDQYCSPKEPEDGKTFNFGMYVEALKFQLT-STTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRDAEHW 424
           SSGQNLQ+SNY+GE+FFAVFIAILGLVLFALLISNIQKYLQS TVKVEQMRINRRDAEHW
Sbjct: 361 SSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           M HRMLPEDLRQRIRRYDQYKWQLNRGV EEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDSHSDV 544
           S MDKQLLDAMCEYLKPVLFTEKS ILQEGDP+DMMLFIMKG LATIITYGWKND+ S V
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIITYGWKNDTFSAV 540

Query: 545 LKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF------- 604
           L AGDFCGEELV WA DP STSLPIS+RTVK+LTEVEAFALKA EL+SV S F       
Sbjct: 541 LNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQRLNS 600

Query: 605 ---------YSHQWKVWAAYKIQEAWHDYCERKRRG-GGDSRFQGALAKTVGASASFGAT 664
                    YSHQW VWAAYKIQEAW +Y ERKRRG  GD RFQ ALAKT GAS  FGAT
Sbjct: 601 KQFQLSVRFYSHQWNVWAAYKIQEAWQEYRERKRRGVRGDGRFQAALAKTAGASVGFGAT 660

Query: 665 LYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIER----DFTILDL 694
           LYASIFISHLLQVVQRDQH++T QL+RVMTLPPPPKP +     + TIL+L
Sbjct: 661 LYASIFISHLLQVVQRDQHRETVQLTRVMTLPPPPKPDDEPDRPNVTILNL 710

BLAST of MC05g0424 vs. ExPASy TrEMBL
Match: A0A6J1E9H2 (cyclic nucleotide-gated ion channel 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431950 PE=4 SV=1)

HSP 1 Score: 1027 bits (2656), Expect = 0.0
Identity = 525/710 (73.94%), Postives = 579/710 (81.55%), Query Frame = 0

Query: 5   MGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVANLGF 64
           MGS  PKS RFG+PTSD H +YG YQN SRLSP SILESI+RK  SA + FRR++ +L F
Sbjct: 1   MGSQIPKSTRFGHPTSDFHDKYGSYQNGSRLSPGSILESIERKFLSAYKSFRRIITHLSF 60

Query: 65  KHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQHQCLT 124
           KH  DQS KKP+FWKKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPV++ + QCLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQCLT 120

Query: 125 MDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSN 184
           +DR LMIIACVLRS +D+FY+LH+IFEFRTGFLPPSLPVFGSGEL+EDPAAIAKRYL SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLFSN 180

Query: 185 FIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYREVTRT 244
           F+IDILS++PLPQLL L IIPAA+GP+PLKTK+ +K+AVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAARGPVPLKTKDLVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRKNNCNSKYLYCGD 304
            GILTETAWSGAAFNLLIYM  SHV GAIWYL SVE++++CW EACRK+ C   +LYCG+
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDNFLYCGN 300

Query: 305 HSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVG 364
             R    +I +YC  KESED KTFNFG+Y+EALKF+LT  TT+F  KFFY FWWALRNV 
Sbjct: 301 QRRQIQPYIDQYCSPKESEDGKTFNFGMYVEALKFQLT-STTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRDAEHW 424
           SSGQNLQ                              KYLQS TVKVEQMRINRRDAEHW
Sbjct: 361 SSGQNLQ------------------------------KYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           M HRMLPEDLRQRIRRYDQYKWQLNRGV EEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDSHSDV 544
           S MDKQLLDAMCEYLKPVLFTEKS ILQEGDP+DM+LFIMKG LATIITYGWKND+ S V
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMVLFIMKGKLATIITYGWKNDTLSGV 540

Query: 545 LKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLF------- 604
           L AGDFCGEELV WAMDP STSLPIS+RTVK+LTEVEAFALKA EL+SV S F       
Sbjct: 541 LNAGDFCGEELVQWAMDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQHLNS 600

Query: 605 ---------YSHQWKVWAAYKIQEAWHDYCERKRRG-GGDSRFQGALAKTVGASASFGAT 664
                    YSHQW VWAAYK+QEAW DY ERKRRG  GD RFQ ALAKT GAS  FGAT
Sbjct: 601 KQFQLSVRFYSHQWNVWAAYKVQEAWQDYRERKRRGVRGDGRFQDALAKTAGASVGFGAT 660

Query: 665 LYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIER----DFTILD 693
           LYASIFISHLLQVVQRDQHQ+T+QL++VMTLPPPPKP +     +FTIL+
Sbjct: 661 LYASIFISHLLQVVQRDQHQETTQLTQVMTLPPPPKPDDEPDSPNFTILN 679

BLAST of MC05g0424 vs. TAIR 10
Match: AT5G53130.1 (cyclic nucleotide gated channel 1 )

HSP 1 Score: 700.7 bits (1807), Expect = 1.2e-201
Identity = 376/677 (55.54%), Postives = 484/677 (71.49%), Query Frame = 0

Query: 38  ASILESIQRKISSAS---EGFRRMVANLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNK 97
           +SI +   R   S+S   + F+R   +  FK   +   K      KILDPQ PFLQ+WNK
Sbjct: 41  SSISDKFYRSFESSSARIKLFKRSYKSYSFK---EAVSKGIGSTHKILDPQGPFLQRWNK 100

Query: 98  IFVLSCVLAVAVDPLFFYIPVMDRQHQCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRT 157
           IFVL+C++AV++DPLFFY+P++D   +CL +D+ + I A VLRSF D+FY+LHIIF+FRT
Sbjct: 101 IFVLACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRT 160

Query: 158 GFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLK 217
           GF+ PS  VFG G LVED   IAKRYLSS+FIIDIL+++PLPQ++ L+IIP  +G   L 
Sbjct: 161 GFIAPSSRVFGRGVLVEDKREIAKRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSLN 220

Query: 218 TKNFMKVAVLLQYLPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVFGAIW 277
           TKN +K  V  QY+PR  RIYPLY+EVTRTSGILTETAW+GAAFNL +YMLASHVFGA W
Sbjct: 221 TKNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGAFW 280

Query: 278 YLFSVEQESRCWHEACRKNN--CNSKYLYCGDHSRPAYGFIKEYCPHKESEDDKTFNFGI 337
           YLFS+E+E+ CW +AC +NN  C SK LYC   +     F+ E CP  ++ +   F+FGI
Sbjct: 281 YLFSIERETVCWKQACERNNPPCISKLLYCDPETAGGNAFLNESCP-IQTPNTTLFDFGI 340

Query: 338 YLEALKFRLTDQTTNFHSKFFYCFWWALRNVGSSGQNLQISNYLGELFFAVFIAILGLVL 397
           +L+AL+  +  ++ +F  KFFYCFWW L+N+ S GQNL+ S Y+ E+ FAVFI+I GLVL
Sbjct: 341 FLDALQSGVV-ESQDFPQKFFYCFWWGLQNLSSLGQNLKTSTYIWEICFAVFISIAGLVL 400

Query: 398 FALLISNIQKYLQSTTVKVEQMRINRRDAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGV 457
           F+ LI N+Q YLQSTT ++E+MR+ RRDAE WM HR+LPE+LR+RIRRY+QYKWQ  RGV
Sbjct: 401 FSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQETRGV 460

Query: 458 NEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQ 517
           +EE L+SNLPKDLRRDIKRHLCLALL +VP+F  MD+QLLDA+C+ L+PVL+TE+S I++
Sbjct: 461 DEENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMDEQLLDALCDRLQPVLYTEESYIVR 520

Query: 518 EGDPVDMMLFIMKGNLATIITYGWKND-SHSDVLKAGDFCGEELVPWAMDPQSTS-LPIS 577
           EGDPVD MLFIM+G L TI T G +    +S+ L AGDFCGEEL+ WA+DP S+S LPIS
Sbjct: 521 EGDPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLGAGDFCGEELLTWALDPHSSSNLPIS 580

Query: 578 TRTVKTLTEVEAFALKAKELQSVVSLF--------------YSHQWKVWAAYKIQEAWHD 637
           TRTV+ L EVEAFALKA +L+ V S F              YS QWK WAA  IQ AW  
Sbjct: 581 TRTVRALMEVEAFALKADDLKFVASQFRRLHSKQLRHTFRYYSQQWKTWAACFIQAAWRR 640

Query: 638 YCERKRRGG---GDSRFQGALAK-TVGASASFGATLYASIFISHLLQVVQRDQHQQTSQL 690
           Y ++K        ++R Q ALAK   G+S S GAT+YAS F +++L+ ++R    +  ++
Sbjct: 641 YIKKKLEESLKEEENRLQDALAKEACGSSPSLGATIYASRFAANILRTIRRSGSVRKPRM 700

BLAST of MC05g0424 vs. TAIR 10
Match: AT1G01340.1 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 677.9 bits (1748), Expect = 8.4e-195
Identity = 363/680 (53.38%), Postives = 470/680 (69.12%), Query Frame = 0

Query: 43  SIQRKISSASEGFRRMVANLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVL 102
           S+ R   +   GF++ ++    K + D         K I++PQ  FLQ WNKIF+ +CV+
Sbjct: 26  SLDRVFKNVKWGFKKPLSFPSHK-DPDHKETSSVTRKNIINPQDSFLQNWNKIFLFACVV 85

Query: 103 AVAVDPLFFYIPVMDRQHQCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLP 162
           A+A+DPLFFYIP++D    CLT+D  L I A +LR+ ID FY++HI+F+FRT ++ PS  
Sbjct: 86  ALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRTLIDAFYIIHIVFQFRTAYIAPSSR 145

Query: 163 VFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVA 222
           VFG GELV+D  AIA +YLSS FIID+LSI+PLPQ++ L +IP+   P+ L TK+++K +
Sbjct: 146 VFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQIVVLAVIPSVNQPVSLLTKDYLKFS 205

Query: 223 VLLQYLPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQE 282
           ++ QY+PR+ R+YPLY EVTRTSGI+TETAW+GAA+NL +YMLASHVFGA+WYL SVE+E
Sbjct: 206 IIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAWNLSLYMLASHVFGALWYLISVERE 265

Query: 283 SRCWHEACRK-NNCNSKYLYCGDHSRPAYGFIKEYCPHKESED---DKTFNFGIYLEALK 342
            RCW EAC K   CN K+LYC +    +  F+   CP  +  D      FNFGI+ +ALK
Sbjct: 266 DRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSCPFLDPGDITNSTIFNFGIFTDALK 325

Query: 343 FRLTDQTTNFHSKFFYCFWWALRNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLIS 402
             +  ++ +F  KFFYCFWW LRN+ + GQNLQ S ++GE+ FA+ I I GLVLFALLI 
Sbjct: 326 SGVV-ESHDFWKKFFYCFWWGLRNLSALGQNLQTSKFVGEIIFAISICISGLVLFALLIG 385

Query: 403 NIQKYLQSTTVKVEQMRINRRDAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELI 462
           N+QKYL+STTV+ E+MR+ +RDAE WM HRMLPEDLR+RIRRY+QY+WQ  RGV EE L+
Sbjct: 386 NMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRRYEQYRWQETRGVEEETLL 445

Query: 463 SNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVD 522
            NLPKDLRRDIKRHLCL LLKKVPLF  MD+QLLDA+C+ L+PVL+TE S +++EGDPV 
Sbjct: 446 RNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDAVCDRLRPVLYTENSYVIREGDPVG 505

Query: 523 MMLFIMKGNLATIITYGWKNDSHSDV-LKAGDFCGEELVPWAMDPQSTS-LPISTRTVKT 582
            MLF+M+G L +  T G ++   + V LKA DFCGE+L+PWA+DPQS+S  PISTRTV+ 
Sbjct: 506 EMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGEDLLPWALDPQSSSHFPISTRTVQA 565

Query: 583 LTEVEAFALKAKELQSVVS--------------LFYSHQWKVWAAYKIQEAWHDYCERKR 642
           LTEVEAFAL A++L+SV S               FYS QW+ W+   IQ AW  YC RK 
Sbjct: 566 LTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFYSVQWRTWSVSFIQAAWRRYCRRKL 625

Query: 643 RGG---GDSRFQGALAK--------TVGASASFGATLYASIFISHLLQVVQRDQHQQTSQ 691
                  + R + ALA         TV +S S G  LYAS F S+ L  ++ +     S 
Sbjct: 626 AKSLRDEEDRLREALASQDKEHNAATVSSSLSLGGALYASRFASNALHNLRHN----ISN 685

BLAST of MC05g0424 vs. TAIR 10
Match: AT1G01340.2 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 677.9 bits (1748), Expect = 8.4e-195
Identity = 363/680 (53.38%), Postives = 470/680 (69.12%), Query Frame = 0

Query: 43  SIQRKISSASEGFRRMVANLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVL 102
           S+ R   +   GF++ ++    K + D         K I++PQ  FLQ WNKIF+ +CV+
Sbjct: 31  SLDRVFKNVKWGFKKPLSFPSHK-DPDHKETSSVTRKNIINPQDSFLQNWNKIFLFACVV 90

Query: 103 AVAVDPLFFYIPVMDRQHQCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLP 162
           A+A+DPLFFYIP++D    CLT+D  L I A +LR+ ID FY++HI+F+FRT ++ PS  
Sbjct: 91  ALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRTLIDAFYIIHIVFQFRTAYIAPSSR 150

Query: 163 VFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVA 222
           VFG GELV+D  AIA +YLSS FIID+LSI+PLPQ++ L +IP+   P+ L TK+++K +
Sbjct: 151 VFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQIVVLAVIPSVNQPVSLLTKDYLKFS 210

Query: 223 VLLQYLPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVFGAIWYLFSVEQE 282
           ++ QY+PR+ R+YPLY EVTRTSGI+TETAW+GAA+NL +YMLASHVFGA+WYL SVE+E
Sbjct: 211 IIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAWNLSLYMLASHVFGALWYLISVERE 270

Query: 283 SRCWHEACRK-NNCNSKYLYCGDHSRPAYGFIKEYCPHKESED---DKTFNFGIYLEALK 342
            RCW EAC K   CN K+LYC +    +  F+   CP  +  D      FNFGI+ +ALK
Sbjct: 271 DRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSCPFLDPGDITNSTIFNFGIFTDALK 330

Query: 343 FRLTDQTTNFHSKFFYCFWWALRNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLIS 402
             +  ++ +F  KFFYCFWW LRN+ + GQNLQ S ++GE+ FA+ I I GLVLFALLI 
Sbjct: 331 SGVV-ESHDFWKKFFYCFWWGLRNLSALGQNLQTSKFVGEIIFAISICISGLVLFALLIG 390

Query: 403 NIQKYLQSTTVKVEQMRINRRDAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELI 462
           N+QKYL+STTV+ E+MR+ +RDAE WM HRMLPEDLR+RIRRY+QY+WQ  RGV EE L+
Sbjct: 391 NMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRRYEQYRWQETRGVEEETLL 450

Query: 463 SNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVD 522
            NLPKDLRRDIKRHLCL LLKKVPLF  MD+QLLDA+C+ L+PVL+TE S +++EGDPV 
Sbjct: 451 RNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDAVCDRLRPVLYTENSYVIREGDPVG 510

Query: 523 MMLFIMKGNLATIITYGWKNDSHSDV-LKAGDFCGEELVPWAMDPQSTS-LPISTRTVKT 582
            MLF+M+G L +  T G ++   + V LKA DFCGE+L+PWA+DPQS+S  PISTRTV+ 
Sbjct: 511 EMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGEDLLPWALDPQSSSHFPISTRTVQA 570

Query: 583 LTEVEAFALKAKELQSVVS--------------LFYSHQWKVWAAYKIQEAWHDYCERKR 642
           LTEVEAFAL A++L+SV S               FYS QW+ W+   IQ AW  YC RK 
Sbjct: 571 LTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFYSVQWRTWSVSFIQAAWRRYCRRKL 630

Query: 643 RGG---GDSRFQGALAK--------TVGASASFGATLYASIFISHLLQVVQRDQHQQTSQ 691
                  + R + ALA         TV +S S G  LYAS F S+ L  ++ +     S 
Sbjct: 631 AKSLRDEEDRLREALASQDKEHNAATVSSSLSLGGALYASRFASNALHNLRHN----ISN 690

BLAST of MC05g0424 vs. TAIR 10
Match: AT4G01010.1 (cyclic nucleotide-gated channel 13 )

HSP 1 Score: 667.5 bits (1721), Expect = 1.1e-191
Identity = 361/693 (52.09%), Postives = 472/693 (68.11%), Query Frame = 0

Query: 26  YGGYQNRSRLSPASILESIQRKISSASEGFRRMVANLGFKHEDDQSRKKPEFWKKILDPQ 85
           YG  +N++R S  ++L++++R +        +   + G  ++   S       K I++PQ
Sbjct: 21  YGYGRNKARPSLNTVLKNVRRGL--------KKPLSFGSHNKKRDSNSSTTTQKNIINPQ 80

Query: 86  KPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQHQCLTMDRLLMIIACVLRSFIDIFYL 145
             FLQ WNKIF+ + V+A+A+DPLFFYIP++D +  CL + R L I A VLR+FID FY+
Sbjct: 81  GSFLQNWNKIFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRTFIDAFYI 140

Query: 146 LHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSIIPLPQLLALVIIP 205
           +HI+F+FRT ++ PS  VFG GELV+DP AIA +YLSS FIID+LSI+PLPQL+ L +IP
Sbjct: 141 IHIVFQFRTAYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQLVVLAVIP 200

Query: 206 AAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYML 265
               P+ L TK+++   +  QY+PR+ RIYPLY EVTRTSGI+TETAW+GAA+NL +YML
Sbjct: 201 NVNKPVSLITKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAWNLSLYML 260

Query: 266 ASHVFGAIWYLFSVEQESRCWHEACRK--NNCNSKYLYCGDHSRPAYGFIKEYCPHKESE 325
           ASHVFGA+WYL SVE+E RCW EAC K    CN ++LYC  +S     F+   CP    +
Sbjct: 261 ASHVFGALWYLISVEREDRCWREACEKIPEVCNFRFLYCDGNSSVRNDFLTTSCPFINPD 320

Query: 326 D---DKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVGSSGQNLQISNYLGELF 385
           D      FNFGI+ +ALK  +  ++ +F  KFFYCFWW LRN+ + GQNL  S ++GE+ 
Sbjct: 321 DITNSTVFNFGIFTDALKSGIV-ESDDFWKKFFYCFWWGLRNLSALGQNLNTSKFVGEII 380

Query: 386 FAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRDAEHWMVHRMLPEDLRQRIRR 445
           FAV I I GLVLFALLI N+QKYL+STTV+ E+MR+ +RDAE WM HRMLP+DLR+RIRR
Sbjct: 381 FAVSICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPDDLRKRIRR 440

Query: 446 YDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAMCEYLK 505
           Y+QYKWQ  RGV EE L+ NLPKDLRRDIKRH CL LLKKVPLF  MD+QLLDA+C+ LK
Sbjct: 441 YEQYKWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLLKKVPLFEIMDEQLLDAVCDKLK 500

Query: 506 PVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDSHSDV-LKAGDFCGEELVPWA 565
           PVL+TE S  ++EGDPV+ MLF+M+G L +  T G +    + V LK  DFCGE+L+ WA
Sbjct: 501 PVLYTENSYAIREGDPVEEMLFVMRGKLMSATTNGGRTGFFNAVYLKPSDFCGEDLLTWA 560

Query: 566 MDPQSTS-LPISTRTVKTLTEVEAFALKAKELQSVVS--------------LFYSHQWKV 625
           +DPQS+S  PISTRTV+ LTEVEAFAL A +L+ V S               FYS QW+ 
Sbjct: 561 LDPQSSSHFPISTRTVQALTEVEAFALAADDLKLVASQFRRLHSKQLQHTFRFYSVQWRT 620

Query: 626 WAAYKIQEAWHDYCERKRRGG---GDSRFQGALAK---TVGASASFGATLYASIFISHLL 685
           W A  IQ AW  +C RK        + RF+ A+ K      +S+S  ATLYAS F S+ L
Sbjct: 621 WGASFIQAAWRRHCRRKLARSLTEEEDRFRNAITKRERNAASSSSLVATLYASRFASNAL 680

Query: 686 QVVQRDQHQQTSQLSRVMTLPPPPKPIERDFTI 692
                 ++ +T+ L  +     PPKP E DF++
Sbjct: 681 ------RNLRTNNLPLL-----PPKPSEPDFSL 693

BLAST of MC05g0424 vs. TAIR 10
Match: AT2G46430.1 (cyclic nucleotide gated channel 3 )

HSP 1 Score: 630.2 bits (1624), Expect = 2.0e-180
Identity = 340/702 (48.43%), Postives = 460/702 (65.53%), Query Frame = 0

Query: 18  PTSDIHVRYGGYQNR-----SRLSPASILESIQRKISSASEGFRRMVANLGFKHEDDQSR 77
           P  +  VR+ G  +      +R S +S++++++R     SE  R     L      ++  
Sbjct: 4   PQRNKFVRFNGNDDEFSTKTTRPSVSSVMKTVRRSFEKGSEKIRTFKRPLSVHSNKNKEN 63

Query: 78  KKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQHQCLTMDRLLMII 137
            K +   ++++P   +LQ WNKIF+L  V+A+A DPLFFYIP +  +  CL +D+ L  I
Sbjct: 64  NKKKKILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPERFCLNLDKKLQTI 123

Query: 138 ACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRYLSSNFIIDILSI 197
           ACV R+FID FY++H++F+F TGF+ PS   FG GEL E    IA RYL S F+ID+LSI
Sbjct: 124 ACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALRYLGSYFLIDLLSI 183

Query: 198 IPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYREVTRTSGILTETA 257
           +P+PQ++ L I+P  + P  L  K  +K  +  QY+PR+ RIYPL++EVTRTSG++TETA
Sbjct: 184 LPIPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKEVTRTSGLVTETA 243

Query: 258 WSGAAFNLLIYMLASHVFGAIWYLFSVEQESRCWHEACRK-NNCNSKYLYCGDHSRPAYG 317
           W+GAA NL +YMLASHVFG+ WYL S+E++ RCW EAC K  NC   YLYC         
Sbjct: 244 WAGAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAYLYCSPTGEDNRL 303

Query: 318 FIKEYCP---HKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWALRNVGSSGQ 377
           F+   CP    +E  +   FNFGI+ +AL+  +  ++ +F  KFFYCFWW LRN+ + GQ
Sbjct: 304 FLNGSCPLIDPEEITNSTVFNFGIFADALQSGVV-ESRDFPKKFFYCFWWGLRNLSALGQ 363

Query: 378 NLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRRDAEHWMVHR 437
           NL+ S + GE+ FA+ I I GLVLFALLI N+QKYLQSTTV+VE+MR+ RRDAE WM HR
Sbjct: 364 NLKTSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMRVKRRDAEQWMSHR 423

Query: 438 MLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMD 497
           MLP+DLR+RIR+Y+QYKWQ  +GV EE L+S+LPKDLR+DIKRHLCL LLKKVP F +MD
Sbjct: 424 MLPDDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLCLKLLKKVPWFQAMD 483

Query: 498 KQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKNDSHSDV-LKA 557
            +LLDA+C  LK VL+TEKS I++EG+PV+ MLFIM+GNL +  TYG +    + V L A
Sbjct: 484 DRLLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYGGRTGFFNSVDLVA 543

Query: 558 GDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVS------------ 617
           GDFCG +L+ WA+DP S+  PIS+RTV+ LTEVE F L A +L+ V +            
Sbjct: 544 GDFCG-DLLTWALDPLSSQFPISSRTVQALTEVEGFLLSADDLKFVATQYRRLHSKQLRH 603

Query: 618 --LFYSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRFQGALAKTV------GASASFGAT 677
              FYS QW+ WAA  IQ AW  +C RK         +G L  T+      G   + GA 
Sbjct: 604 MFRFYSVQWQTWAACFIQAAWKRHCRRKLSKALREE-EGKLHNTLQNDDSGGNKLNLGAA 663

Query: 678 LYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIERDF 690
           +YAS F SH L+ ++ +   + S+   ++TL  P KP + +F
Sbjct: 664 IYASRFASHALRNLRANAAARNSRFPHMLTL-LPQKPADPEF 701