MC05g0336 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0336 vs. ExPASy Swiss-Prot
Match: O65272 (K(+) efflux antiporter 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA2 PE=1 SV=2)

HSP 1 Score: 1439.9 bits (3726), Expect = 0.0e+00
Identity = 828/1115 (74.26%), Postives = 928/1115 (83.23%), Query Frame = 0

Query: 105  CQGNDSLAFIDGNGRNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETD-APTVDE 164
            CQ +DS+  + GN RN+E+ +  D+   +          S+E   E +  E D AP+++E
Sbjct: 98   CQSSDSVGDLVGNDRNLEFAEGSDDREVT---------FSKE---EKDTREQDSAPSLEE 157

Query: 165  LRELLQKAMKELEVARLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNE 224
            LR+LL KA KELEVA LNSTMFEEKAQ+ISE AIAL+DEAA AWNDVN TL+ VQ+ V+E
Sbjct: 158  LRDLLNKATKELEVASLNSTMFEEKAQRISEVAIALKDEAASAWNDVNQTLNVVQEAVDE 217

Query: 225  EYAAKEAVQKATMALSLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLI 284
            E  AKEAVQKATMALSLAEARLQVA+ESLE E G N S E    SE     K++EE LL 
Sbjct: 218  ESVAKEAVQKATMALSLAEARLQVALESLEAE-GYNTSEE----SEVRDGVKDKEEALLS 277

Query: 285  AKEDIKECRENLENCDSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLL 344
            AK DIKEC+ENL +C+ +L+RLQ  K+ELQKEVDRLNE AE AQ++ALKAEEDVANIM+L
Sbjct: 278  AKADIKECQENLASCEEQLRRLQVKKDELQKEVDRLNEAAERAQISALKAEEDVANIMVL 337

Query: 345  AEQAVAFELEAAQRVSDAEIALQKLEKSSSNSFVDVSETTQG--LNVIEEVVNEENKAVI 404
            AEQAVAFELEA QRV+DAEIALQ+ EK+   S     ETTQG  L+    +V E+   V+
Sbjct: 338  AEQAVAFELEATQRVNDAEIALQRAEKTLFGS--QTQETTQGKVLDGKNTIVGEDE--VL 397

Query: 405  GISGDIAVEMDRDVAFSADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVES 464
                D++ + +RD+      + V S  GT S         Y SDNENGK ++D  KE E 
Sbjct: 398  SEIVDVSHQAERDLVV----VGVSSDVGTQS---------YESDNENGKPTADFAKEAEG 457

Query: 465  GAEKS-ILSQAKKQETQKDLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASV 524
             AEKS  +   KKQE QKDL RE S  N  K  LKKSSRFF ASFFS  GDGT    A+V
Sbjct: 458  EAEKSKNVVLTKKQEVQKDLPRESSSHNGTKTSLKKSSRFFPASFFSSNGDGT----ATV 517

Query: 525  FQGLLDSTKKQLPKLVVGLVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPL 584
            F+ L++S K+Q PKL++G  LLG G+ I++    RN Q+ QQP++++TS ++VS +TKPL
Sbjct: 518  FESLVESAKQQWPKLILGFTLLGAGVAIYSNGVGRNNQLPQQPNIVSTSAEDVSSSTKPL 577

Query: 585  FQQLRKLPKRVKELISKLPHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYL 644
             +Q++KLPKR+K+L+   P QE   +EASLLD+LWLLLASVIFVP FQK+PGGSPVLGYL
Sbjct: 578  IRQMQKLPKRIKKLLEMFPQQEVNEEEASLLDVLWLLLASVIFVPLFQKIPGGSPVLGYL 637

Query: 645  AAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVL 704
            AAGILIGPYGLSIIR+VHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVL
Sbjct: 638  AAGILIGPYGLSIIRNVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVL 697

Query: 705  VTAVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLF 764
            VTA V+GL+ H V GQ GPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLF
Sbjct: 698  VTAAVIGLITHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLF 757

Query: 765  QDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYK 824
            QDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAA+KA VAI+ IIAGGRLLLRPIYK
Sbjct: 758  QDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLLLRPIYK 817

Query: 825  QIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPY 884
            QIAEN+NAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPY
Sbjct: 818  QIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPY 877

Query: 885  RGLLLGLFFMTVGMSIDPKLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRV 944
            RGLLLGLFFMTVGMSIDPKLLL+NFP+I G+LGLL+ GKTILV ++G+LFGISIISA+RV
Sbjct: 878  RGLLLGLFFMTVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVRV 937

Query: 945  GLLLAPGGEFAFVAFGEAVNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQH 1004
            GLLLAPGGEFAFVAFGEAVNQGIM+  LSSLLFLVVGISMALTPWLAAGGQLIASRFE  
Sbjct: 938  GLLLAPGGEFAFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGGQLIASRFELQ 997

Query: 1005 DVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDL 1064
            DVRSLLPVESETDDLQ HIIICGFGR+GQIIAQLLSERLIPFVALDV SDRVA+GR+LDL
Sbjct: 998  DVRSLLPVESETDDLQGHIIICGFGRIGQIIAQLLSERLIPFVALDVSSDRVAIGRSLDL 1057

Query: 1065 PVYFGDAGSREVLHKVGAERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHG 1124
            PVYFGDAGSREVLHK+GA+RACAAAI LD+PGANYR VWALSKYFPNVKTFVRAHDVDHG
Sbjct: 1058 PVYFGDAGSREVLHKIGADRACAAAIALDTPGANYRCVWALSKYFPNVKTFVRAHDVDHG 1117

Query: 1125 LNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASG 1184
            LNLEKAGATAVVPETLEPSLQLAAAVLAQAKLP SEIA TINEFRSRHLSEL ELCEASG
Sbjct: 1118 LNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPTSEIATTINEFRSRHLSELAELCEASG 1174

Query: 1185 SSLGYGFSRIMSKPKIQT-SDSSDENQVTEGTLAI 1215
            SSLGYGFSR  SKPK  + S++SD+NQ+ EGTLAI
Sbjct: 1178 SSLGYGFSRSTSKPKPPSPSETSDDNQIIEGTLAI 1174

BLAST of MC05g0336 vs. ExPASy Swiss-Prot
Match: Q9ZTZ7 (K(+) efflux antiporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA1 PE=1 SV=2)

HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 819/1112 (73.65%), Postives = 916/1112 (82.37%), Query Frame = 0

Query: 105  CQGNDSLAFIDGNGRNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDEL 164
            CQG++SL   D N   +     G+ S SS  T+      +R         E D  +++EL
Sbjct: 98   CQGHESLGNADSNDHRI-----GESSESSDETEATDLKDAR--------VENDTDSLEEL 157

Query: 165  RELLQKAMKELEVARLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEE 224
            +ELL KA+KELEVARLNSTMFEEKAQ+ISE AIAL+DEAA AW DVN TLD ++  V EE
Sbjct: 158  KELLHKAIKELEVARLNSTMFEEKAQRISERAIALKDEAATAWLDVNKTLDVIRDTVYEE 217

Query: 225  YAAKEAVQKATMALSLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIA 284
              AKEAVQ ATMALSLAEARLQV +ESLE    GND P  +  +E  ID  ++EE LL A
Sbjct: 218  ALAKEAVQTATMALSLAEARLQVIVESLE-AGAGNDIPHVSEETEETIDVNDKEEALLAA 277

Query: 285  KEDIKECRENLENCDSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLA 344
            K+DIKEC+ NL+NC+S+L  L S K+ELQKEVD+LNE AET Q+++LKAEEDV NIM LA
Sbjct: 278  KDDIKECQVNLDNCESQLSALLSKKDELQKEVDKLNEFAETIQISSLKAEEDVTNIMKLA 337

Query: 345  EQAVAFELEAAQRVSDAEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGIS 404
            EQAVAFELEA QRV+DAEIALQ+ EKS S S     E TQG    EE   E+   + G  
Sbjct: 338  EQAVAFELEATQRVNDAEIALQRAEKSLSIS--QTPEETQGQLSDEETSQEDAMVLSGNV 397

Query: 405  GDIAVEMDRDVAFSADSLAVKSLPGTFSD-----SEDSDQPYYLSDNENGKLSSDSLKEV 464
             D+  +++++     D   V+       D     ++   QPY  SD+ENGK S +S K V
Sbjct: 398  EDVTHQVEKESPKDGDLPVVQITAELVPDIVGQRNKKLTQPYESSDHENGKPSVESSKVV 457

Query: 465  ESGAEK-SILSQAKKQETQKDLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPA 524
            E+ +EK  I  Q KKQETQKDL +EGS LN+PKA   KSSRFFSASFFS   DGT    A
Sbjct: 458  EADSEKPKINVQTKKQETQKDLPKEGSSLNSPKASFNKSSRFFSASFFSSNPDGT----A 517

Query: 525  SVFQGLLDSTKKQLPKLVVGLVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTK 584
            +VF  L+ S K+Q PKLV+GL LLG G+T+++     N Q+ QQPDV +TST++VS +TK
Sbjct: 518  TVFGSLVGSVKQQWPKLVLGLALLGAGLTLYSNGVGGNNQLLQQPDVTSTSTEDVSSSTK 577

Query: 585  PLFQQLRKLPKRVKELISKLPHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLG 644
            PL +Q++KLPKR+K+L+  +PHQE   +EASL D LWLLLASVIFVP FQK+PGGSPVLG
Sbjct: 578  PLIRQVQKLPKRIKKLLEMIPHQEVNEEEASLFDFLWLLLASVIFVPLFQKIPGGSPVLG 637

Query: 645  YLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQ 704
            YLAAGILIGPYGLSIIR+VHGT+AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQ
Sbjct: 638  YLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQ 697

Query: 705  VLVTAVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVL 764
            VLVTA VVGL+AH V GQ GPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRA+FSVL
Sbjct: 698  VLVTAAVVGLLAHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRASFSVL 757

Query: 765  LFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPI 824
            LFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKA VAI+AIIAGGRLLLRPI
Sbjct: 758  LFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAAVAITAIIAGGRLLLRPI 817

Query: 825  YKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIA 884
            YKQIAEN+NAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIA
Sbjct: 818  YKQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIA 877

Query: 885  PYRGLLLGLFFMTVGMSIDPKLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISAL 944
            PYRGLLLGLFFMTVGMSIDPKLLLSNFPVI G+LGLLI GKT+LV ++G+LFGISIISA+
Sbjct: 878  PYRGLLLGLFFMTVGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMGKLFGISIISAI 937

Query: 945  RVGLLLAPGGEFAFVAFGEAVNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFE 1004
            RVGLLLAPGGEFAFVAFGEAVNQGIMS  LSSLLFLVVGISMA+TPWLAAGGQLIASRFE
Sbjct: 938  RVGLLLAPGGEFAFVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWLAAGGQLIASRFE 997

Query: 1005 QHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRAL 1064
             HDVRSLLPVESETDDLQ HIIICGFGRVGQIIAQLLSERLIPFVALDV SDRV +GR+L
Sbjct: 998  LHDVRSLLPVESETDDLQGHIIICGFGRVGQIIAQLLSERLIPFVALDVSSDRVTIGRSL 1057

Query: 1065 DLPVYFGDAGSREVLHKVGAERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVD 1124
            DLPVYFGDAGS+EVLHK+GA RACAA + LD+PGANYR VWALSK++PNVKTFVRAHDV 
Sbjct: 1058 DLPVYFGDAGSKEVLHKIGAGRACAAVVALDAPGANYRCVWALSKFYPNVKTFVRAHDVV 1117

Query: 1125 HGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEA 1184
            HGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLP SEIA TINEFR+RHLSELTELCEA
Sbjct: 1118 HGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPTSEIANTINEFRTRHLSELTELCEA 1177

Query: 1185 SGSSLGYGFSRIMSKPKIQTSDSSDENQVTEG 1211
            SGSSLGYG+SR  SKPK Q SD+S +NQ+ EG
Sbjct: 1178 SGSSLGYGYSR-TSKPKPQPSDASGDNQIIEG 1188

BLAST of MC05g0336 vs. ExPASy Swiss-Prot
Match: Q9M0Z3 (K(+) efflux antiporter 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA3 PE=1 SV=2)

HSP 1 Score: 335.5 bits (859), Expect = 2.5e-90
Identity = 223/601 (37.10%), Postives = 332/601 (55.24%), Query Frame = 0

Query: 612  DMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFL 671
            D L  L+ +VI VP F+ L   SP+LG+  AG+++  +GL  IR++   K ++E+G++FL
Sbjct: 107  DTLTFLMVTVIIVPAFRILK-ASPILGFFFAGVVLNQFGL--IRNLTDVKVLSEWGILFL 166

Query: 672  LFNIGLELSVERLSSMKKYVFGLGSAQVLVT-------------AVVVGLVAHIVCGQPG 731
            LF +GLELS+ RL ++ K+ FG+G  QVL+              A+   ++  +   +P 
Sbjct: 167  LFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHSRPD 226

Query: 732  -------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLIL 791
                     A+VIG  L+LSS+A VLQ+L E+GE  +R G AT  +LL QD+AVV LL++
Sbjct: 227  LVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVI 286

Query: 792  IPLISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIF 851
            +P++        IG ++I   L   + KA+  +  +  GG+  LR I++ +AE +++E F
Sbjct: 287  LPVLESQD----IGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAF 346

Query: 852  SANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMT 911
             A  LL + GTSL+T   G S  LGAFLAG LLAET F  Q+E+DI P+RGLLLGLFF+T
Sbjct: 347  VALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVT 406

Query: 912  VGMSIDPKLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFA 971
             G SID ++L   +P +   LG LI  KT+++  +G   G++I  ++RVG LL+ GGEFA
Sbjct: 407  TGTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFA 466

Query: 972  FVAFGEAVNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESE 1031
            FV F  A   G++ + L+ LL +VV +SMALTP+L   G+  A   ++         E  
Sbjct: 467  FVVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDV 526

Query: 1032 TDDLQDHIIICGFGRVGQIIAQLLSERLI--------PFVALDVRSDRVAVGRALDLPVY 1091
              D+ + I+I GFG++GQ++A  LS  L+        P++  D+    V   R L  P+ 
Sbjct: 527  NFDVSESIVIIGFGQMGQVLANFLSTPLVSDSDLVGWPYIGFDLNPAVVKESRKLGFPIL 586

Query: 1092 FGDAGSREVLHKVGAERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNL 1151
            +GD     VL   G     A  I           V  L   FP    + RA D+ H L L
Sbjct: 587  YGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGSPIYARAQDLPHLLEL 646

Query: 1152 EKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSL 1185
            +KAGAT  + E  E SLQL + +L    +   +++     FR     +  E   AS ++ 
Sbjct: 647  KKAGATDAILENAETSLQLGSKLLTGFGVMSDDVSFLSKVFRDSMEIQAQEEITASETNA 700

BLAST of MC05g0336 vs. ExPASy Swiss-Prot
Match: A9MN27 (Glutathione-regulated potassium-efflux system protein KefB OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) OX=41514 GN=kefB PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 2.1e-81
Identity = 213/564 (37.77%), Postives = 325/564 (57.62%), Query Frame = 0

Query: 617  LLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIG 676
            L A+V+ VP   +L G   VLGYL AGI IGP+GL  I  V      +E GVVFL+F IG
Sbjct: 14   LFAAVVAVPLAARL-GIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIG 73

Query: 677  LELSVERLSSMKKYVFGLGSAQVLVT-AVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAV 736
            LEL+  RL  +++ +FG+G+AQVL++ AV+ GL+  ++      AAIV G GLA+SSTA+
Sbjct: 74   LELNPSRLWQLRRSIFGVGAAQVLLSAAVLAGLL--MLADFLWQAAIVGGIGLAMSSTAM 133

Query: 737  VLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGL 796
             LQ+++E+G + S  G+  FSVLLFQDLAV+  L L+PL++ ++ +    F+     +G+
Sbjct: 134  ALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDWFK-----VGM 193

Query: 797  AAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMAL 856
                 ++A + ++ GGR LLRP+++ IA +   E+F+A TLL++LG +L     GLSMAL
Sbjct: 194  ----KVLAFAVMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGAALFMDALGLSMAL 253

Query: 857  GAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVISGSLGLL 916
            G F+AG+LLAE+E+  ++E+ I P++GLLLGLFF++VGMS++  +L ++   ++ S+ +L
Sbjct: 254  GTFIAGVLLAESEYRHELENAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVAASVVIL 313

Query: 917  IGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQGIMSSHLSSLLFLV 976
            +  K + + L+ RL+GI     ++   +L+ GGEFAFV F  A +Q +      +LL + 
Sbjct: 314  VVIKMLALYLLARLYGIRSSERMQFASVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVA 373

Query: 977  VGISMALTPWLAAGGQLIASRF----EQHDVRSLLPVESETDDLQDHIIICGFGRVGQII 1036
            V +SM  TP L  G     SR     E+HD +  +      +D +  +I+ GFGR GQ+I
Sbjct: 374  VTLSMMTTPLLMKGIDKWLSRRLNGPEEHDEKPWV------EDDKPQVIVVGFGRFGQVI 433

Query: 1037 AQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDSP 1096
            A+LL    +    L+     V + R     VY+GDA   E+L   GAE A +  IT + P
Sbjct: 434  ARLLMANKVRITVLERDIGAVNLMRKYGYKVYYGDATQVELLRSAGAEAAESIVITCNEP 493

Query: 1097 GANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAK 1156
                + V    ++FP++    RA        L +AG T    ET   +L+L    L    
Sbjct: 494  EDTMKLVELCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVSLG 553

Query: 1157 LPMSEIAATINEFRSRHLSELTEL 1176
            +   +       FR   +  L EL
Sbjct: 554  MHPHQAQRAQLHFRRLDMRMLREL 559

BLAST of MC05g0336 vs. ExPASy Swiss-Prot
Match: B5XN76 (Glutathione-regulated potassium-efflux system protein KefB OS=Klebsiella pneumoniae (strain 342) OX=507522 GN=kefB PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.8e-81
Identity = 210/567 (37.04%), Postives = 320/567 (56.44%), Query Frame = 0

Query: 610  LLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVV 669
            LL  +  L A+VI VP   +L G   VLGYL AGI IGP+GL  I  V      +E GVV
Sbjct: 7    LLAGVLFLFAAVIAVPLASRL-GIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVV 66

Query: 670  FLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPAAIVIGNGLA 729
            FL+F IGLEL+  +L  ++  +FG+G+AQV+++A ++G +  +  G    AA+V G GLA
Sbjct: 67   FLMFIIGLELNPAKLWRLRSSIFGVGAAQVMLSAAILGGLL-MTTGFSWQAAVVGGIGLA 126

Query: 730  LSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAI 789
            +SSTA+ LQ+++E+G S S  G+  FSVLLFQDLAV+  L L+PL++ ++ +        
Sbjct: 127  MSSTAMALQLMREKGMSRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADE-------- 186

Query: 790  AEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARA 849
                 L     ++A + ++ GGR LLRP+++ IA +   E+F+A TLL++LG++L     
Sbjct: 187  -HVNWLTVGMKVLAFAGMLIGGRYLLRPVFRFIASSGVREVFTAATLLLVLGSALFMEAL 246

Query: 850  GLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVIS 909
            GLSMALG F+AG+LLAE+E+  ++E  I P++GLLLGLFF++VGM+++  +L ++   ++
Sbjct: 247  GLSMALGTFIAGVLLAESEYRHELEIAIDPFKGLLLGLFFISVGMALNLGVLYTHLLWVA 306

Query: 910  GSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVNQGIMSSHLS 969
             S+ +L+  K +++ L+ RL+G+     ++   +L+ GGEFAFV F    +Q +      
Sbjct: 307  VSVAVLVAVKMLVLYLLARLYGLRSSERMQFAGVLSQGGEFAFVLFSLPASQRLFQHDQM 366

Query: 970  SLLFLVVGISMALTPWLAAG-GQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFGRVG 1029
            +LL + V +SM  TP L  G  +L++ R    D     P     +D +  +II GFGR G
Sbjct: 367  ALLLVAVTLSMMTTPLLMKGIDKLLSRRLNPADDTDEAP---WVEDDKPQVIIVGFGRFG 426

Query: 1030 QIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITL 1089
            Q+I +LL    +    L+     V + R     VYFGDA   E+L   GAE A +  IT 
Sbjct: 427  QVIGRLLMANKMRITVLERDISAVNLMRNYGYKVYFGDATQLELLRSAGAEEAQSIVITC 486

Query: 1090 DSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLA 1149
            + P    R V    ++FP++    RA        L +AG T    ET   +L+L    L 
Sbjct: 487  NEPEDTMRLVEMCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKALI 546

Query: 1150 QAKLPMSEIAATINEFRSRHLSELTEL 1176
               +   +       FR   +  L EL
Sbjct: 547  TLGMHPHQAQRAQLHFRRLDMRMLREL 559

BLAST of MC05g0336 vs. NCBI nr
Match: XP_022147478.1 (K(+) efflux antiporter 2, chloroplastic [Momordica charantia])

HSP 1 Score: 2231 bits (5781), Expect = 0.0
Identity = 1210/1214 (99.67%), Postives = 1211/1214 (99.75%), Query Frame = 0

Query: 1    MDLSCNIWKQNVFSERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFIAS 60
            MDLSCNIWKQNVFSERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFIAS
Sbjct: 1    MDLSCNIWKQNVFSERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFIAS 60

Query: 61   SNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNGRN 120
            SNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNGRN
Sbjct: 61   SNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNGRN 120

Query: 121  VEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVARL 180
            VEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVARL
Sbjct: 121  VEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVARL 180

Query: 181  NSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSL 240
            NSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSL
Sbjct: 181  NSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSL 240

Query: 241  AEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENCDS 300
            AEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENCDS
Sbjct: 241  AEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENCDS 300

Query: 301  ELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSD 360
            ELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSD
Sbjct: 301  ELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSD 360

Query: 361  AEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFSAD 420
            AEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFSAD
Sbjct: 361  AEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFSAD 420

Query: 421  SLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQKDL 480
            SLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQKDL
Sbjct: 421  SLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQKDL 480

Query: 481  TREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVGLV 540
            TREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVGLV
Sbjct: 481  TREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVGLV 540

Query: 541  LLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKLPH 600
            LLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKLPH
Sbjct: 541  LLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKLPH 600

Query: 601  QEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGT 660
            QE   +EASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGT
Sbjct: 601  QEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGT 660

Query: 661  KAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPA 720
            KAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPA
Sbjct: 661  KAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPA 720

Query: 721  AIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSS 780
            AIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSS
Sbjct: 721  AIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSS 780

Query: 781  KGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVIL 840
            KGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVIL
Sbjct: 781  KGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVIL 840

Query: 841  GTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKL 900
            GTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKL
Sbjct: 841  GTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKL 900

Query: 901  LLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVN 960
            LLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVN
Sbjct: 901  LLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVN 960

Query: 961  QGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHII 1020
            QGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHII
Sbjct: 961  QGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHII 1020

Query: 1021 ICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAER 1080
            ICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAER
Sbjct: 1021 ICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAER 1080

Query: 1081 ACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSL 1140
            ACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSL
Sbjct: 1081 ACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSL 1140

Query: 1141 QLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSD 1200
            QLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSD
Sbjct: 1141 QLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSD 1200

Query: 1201 SSDENQVTEGTLAI 1214
            SSDENQVTEGTLAI
Sbjct: 1201 SSDENQVTEGTLAI 1214

BLAST of MC05g0336 vs. NCBI nr
Match: XP_022980192.1 (K(+) efflux antiporter 2, chloroplastic-like [Cucurbita maxima] >XP_022980193.1 K(+) efflux antiporter 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 2024 bits (5244), Expect = 0.0
Identity = 1110/1216 (91.28%), Postives = 1146/1216 (94.24%), Query Frame = 0

Query: 1    MDLSCNIWKQNVF--SERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFI 60
            MDLSCNIWKQNV   SERT+CKTLDQFG   VLRSR +DAK+ GGSRV+YKVP KRNR +
Sbjct: 1    MDLSCNIWKQNVLCGSERTHCKTLDQFGLRYVLRSRALDAKMSGGSRVIYKVPNKRNRIV 60

Query: 61   ASSNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNG 120
            +SS+S +LSLVCA KFD  LQI KHKRF NC    NGR+MGMVHSECQ NDSLAFIDGNG
Sbjct: 61   SSSDSFHLSLVCASKFDGALQISKHKRFLNCN--YNGRKMGMVHSECQSNDSLAFIDGNG 120

Query: 121  RNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVA 180
            RN+EYVD GDE SSS PTDGVGS GSREAGGE EA ET APTVDELRELLQKAMKELEVA
Sbjct: 121  RNMEYVDTGDEGSSSVPTDGVGSAGSREAGGETEAVETVAPTVDELRELLQKAMKELEVA 180

Query: 181  RLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240
            RLNSTMFEEKAQKISE AIALQDEA HA NDVNSTLDSVQQIVNEEYAAKEAVQKATMAL
Sbjct: 181  RLNSTMFEEKAQKISETAIALQDEATHARNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240

Query: 241  SLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENC 300
            SLAEARLQVAIES+E  KGGND PET+I SEREID K+E++VLL+A+EDIKECR NLENC
Sbjct: 241  SLAEARLQVAIESMELAKGGNDFPETSINSEREIDGKDEQDVLLVAQEDIKECRANLENC 300

Query: 301  DSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360
            D+ELKRLQS KEELQKEVDRLNELAE AQLNALKAEEDVANIMLLAEQAVAFELEAAQRV
Sbjct: 301  DAELKRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360

Query: 361  SDAEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFS 420
            SDAEIALQK+EKS S+SFVD SE TQGLNVIEEV NE+NKAV  ISGDIAVEMDRD+  S
Sbjct: 361  SDAEIALQKVEKSLSSSFVDTSEITQGLNVIEEVENEDNKAVPEISGDIAVEMDRDLPLS 420

Query: 421  ADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQK 480
             DSL +KSLPG+FSDSEDSDQPYYLSD+ENGKLSSDS KEVESGAEKSILSQAKK ETQK
Sbjct: 421  GDSLVIKSLPGSFSDSEDSDQPYYLSDSENGKLSSDSSKEVESGAEKSILSQAKKPETQK 480

Query: 481  DLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVG 540
            DLTREGSP+N+PKALLKKSSRFFSASFFSF  DGTEFTPA VFQG LDSTKKQLPKLVVG
Sbjct: 481  DLTREGSPVNSPKALLKKSSRFFSASFFSFAVDGTEFTPALVFQGFLDSTKKQLPKLVVG 540

Query: 541  LVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKL 600
             VL G GI I A RAERN  MFQQPDV+TTSTDEVSLNTKPLFQQLRKLPKRVK+LI+KL
Sbjct: 541  AVLFGAGIAILANRAERNSLMFQQPDVVTTSTDEVSLNTKPLFQQLRKLPKRVKKLIAKL 600

Query: 601  PHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660
            PHQE   +EASLLD+LWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH
Sbjct: 601  PHQEVNEEEASLLDVLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660

Query: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPG 720
            GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ G
Sbjct: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAG 720

Query: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780
            PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN
Sbjct: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780

Query: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840
            SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV
Sbjct: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840

Query: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900
            ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP
Sbjct: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900

Query: 901  KLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEA 960
            KLLLSNFPVI GSLGLLIGGKTILVALVGRLFG+SIISALRVGLLLAPGGEFAFVAFGEA
Sbjct: 901  KLLLSNFPVIMGSLGLLIGGKTILVALVGRLFGVSIISALRVGLLLAPGGEFAFVAFGEA 960

Query: 961  VNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020
            VNQGIMSS LSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH
Sbjct: 961  VNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020

Query: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080
            IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA
Sbjct: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080

Query: 1081 ERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140
            ERACAAAITLD+PGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP
Sbjct: 1081 ERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140

Query: 1141 SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT 1200
            SLQLAAAVLAQAKLP SEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPK+QT
Sbjct: 1141 SLQLAAAVLAQAKLPTSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKVQT 1200

Query: 1201 SDSSDENQVTEGTLAI 1214
            SDSSDENQV+EGTLAI
Sbjct: 1201 SDSSDENQVSEGTLAI 1214

BLAST of MC05g0336 vs. NCBI nr
Match: KAG7018984.1 (K(+) efflux antiporter 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2018 bits (5228), Expect = 0.0
Identity = 1108/1216 (91.12%), Postives = 1144/1216 (94.08%), Query Frame = 0

Query: 1    MDLSCNIWKQNVF--SERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFI 60
            MDLSC+IWKQNV   SERT+CKTLDQFG   VLRSR +DAKL GGSRV+YKVP KRNR +
Sbjct: 1    MDLSCHIWKQNVLCGSERTHCKTLDQFGLRYVLRSRALDAKLSGGSRVIYKVPNKRNRIV 60

Query: 61   ASSNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNG 120
            +SS+S +LSLVC  KFD  LQI KHKRF NC    NGR+MGMVHSECQ NDSLAFIDGNG
Sbjct: 61   SSSDSIHLSLVCGSKFDGALQISKHKRFLNCN--YNGRKMGMVHSECQSNDSLAFIDGNG 120

Query: 121  RNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVA 180
            RN+EYVD GDE SSS PTDGVGS GSREAGGE EA ET APTVDELRELLQKA+KELEVA
Sbjct: 121  RNMEYVDTGDEGSSSVPTDGVGSAGSREAGGEAEAVETVAPTVDELRELLQKAIKELEVA 180

Query: 181  RLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240
            RLNSTMFEEKAQKISE AIALQDEA HA NDVNSTLDSVQQIV EEYAAKEAVQKATMAL
Sbjct: 181  RLNSTMFEEKAQKISETAIALQDEATHARNDVNSTLDSVQQIVKEEYAAKEAVQKATMAL 240

Query: 241  SLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENC 300
            SLAEARLQVAIES+E  KGGND PET+I SE EID K+E+EVLL+A+EDIKECR NLENC
Sbjct: 241  SLAEARLQVAIESMELAKGGNDFPETSINSEGEIDGKDEQEVLLVAQEDIKECRANLENC 300

Query: 301  DSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360
            D+ELKRLQS KEELQKEVDRLNELAE AQLNALKAEEDVANIMLLAEQAVAFELEAAQRV
Sbjct: 301  DAELKRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360

Query: 361  SDAEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFS 420
            SDAEIALQK+EKS S+SFVD SE TQGLNVIEEV NE+NKAV  ISGDIAVEMDRD+  S
Sbjct: 361  SDAEIALQKVEKSLSSSFVDTSEITQGLNVIEEVENEDNKAVPEISGDIAVEMDRDLPLS 420

Query: 421  ADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQK 480
             DSL +KSLPG+FSDSEDSDQPYYLSD+ENGKLSSDS KEVESGAEKSILSQAKK ETQK
Sbjct: 421  GDSLVIKSLPGSFSDSEDSDQPYYLSDSENGKLSSDSSKEVESGAEKSILSQAKKPETQK 480

Query: 481  DLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVG 540
            DLTREGSP+N+PKALLKKSSRFFSASFFSF  DGTEFTPA VFQG LDSTKKQLPKLVVG
Sbjct: 481  DLTREGSPVNSPKALLKKSSRFFSASFFSFAVDGTEFTPALVFQGFLDSTKKQLPKLVVG 540

Query: 541  LVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKL 600
             VL G GI+I A RAERN  MFQQPDV+TTSTDEVSLNTKPLFQQLRKLPKRVK+LI+KL
Sbjct: 541  AVLFGAGISILANRAERNSLMFQQPDVVTTSTDEVSLNTKPLFQQLRKLPKRVKKLIAKL 600

Query: 601  PHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660
            PHQE   +EASLLD+LWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH
Sbjct: 601  PHQEVNEEEASLLDVLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660

Query: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPG 720
            GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ G
Sbjct: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAG 720

Query: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780
            PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN
Sbjct: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780

Query: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840
            SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV
Sbjct: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840

Query: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900
            ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP
Sbjct: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900

Query: 901  KLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEA 960
            KLLLSNFPVI GSLGLLIGGKTILVALVGRLFG+SIISALRVGLLLAPGGEFAFVAFGEA
Sbjct: 901  KLLLSNFPVIMGSLGLLIGGKTILVALVGRLFGVSIISALRVGLLLAPGGEFAFVAFGEA 960

Query: 961  VNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020
            VNQGIMSS LSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH
Sbjct: 961  VNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020

Query: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080
            IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA
Sbjct: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080

Query: 1081 ERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140
            ERACAAAITLD+PGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP
Sbjct: 1081 ERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140

Query: 1141 SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT 1200
            SLQLAAAVLAQAKLP SEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPK+QT
Sbjct: 1141 SLQLAAAVLAQAKLPTSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKVQT 1200

Query: 1201 SDSSDENQVTEGTLAI 1214
            SDSSDENQVTEGTLAI
Sbjct: 1201 SDSSDENQVTEGTLAI 1214

BLAST of MC05g0336 vs. NCBI nr
Match: XP_038878214.1 (K(+) efflux antiporter 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 2018 bits (5227), Expect = 0.0
Identity = 1108/1216 (91.12%), Postives = 1141/1216 (93.83%), Query Frame = 0

Query: 1    MDLSCNIWKQNVF--SERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFI 60
            MDLSC+ WKQNV   SERT CKT DQFG S+VLR+RIIDAKL GGSRV YKVPKKRNR +
Sbjct: 1    MDLSCHTWKQNVLCGSERTSCKTFDQFGPSSVLRNRIIDAKLTGGSRVSYKVPKKRNRIV 60

Query: 61   ASSNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNG 120
            ASSNSN+LSLVCA KFD  LQIF+HK+F N  NKI+GRRMGMV  ECQ NDSLAFIDGNG
Sbjct: 61   ASSNSNHLSLVCASKFDGALQIFRHKKFLNWNNKISGRRMGMVQLECQNNDSLAFIDGNG 120

Query: 121  RNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVA 180
            RN+E V+ GDE SSS PTDGVGS GSRE GGE E  E D PTVDELRELLQKAMKELEVA
Sbjct: 121  RNMECVNTGDEGSSSEPTDGVGSAGSREVGGEAETVEADVPTVDELRELLQKAMKELEVA 180

Query: 181  RLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240
            RLNSTMFEEKAQKISEAAIALQDEA  A NDVNSTLDSVQQIVNEEYAAKEAVQKATMAL
Sbjct: 181  RLNSTMFEEKAQKISEAAIALQDEATIARNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240

Query: 241  SLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENC 300
            SLAEARLQVAIESLE  KGGND PET+I SE  ID KEE E+LL+A+EDIKECR NLENC
Sbjct: 241  SLAEARLQVAIESLELAKGGNDFPETSIDSEGTIDGKEEREMLLVAQEDIKECRANLENC 300

Query: 301  DSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360
            D+ELKRLQS KEELQKEVDRLNELAE AQLNALKAEEDVANIM LAEQAVAFELEAAQRV
Sbjct: 301  DAELKRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMQLAEQAVAFELEAAQRV 360

Query: 361  SDAEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFS 420
            SDAEIALQK+EKS S+SFVD  E TQG NVIEEV  E+NKAV+ ISGDIAVEMDRD+  S
Sbjct: 361  SDAEIALQKVEKSLSSSFVDTLEITQGSNVIEEVETEDNKAVLEISGDIAVEMDRDLPLS 420

Query: 421  ADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQK 480
             DSL++KSLPG+FSDSEDSDQPYYLSD+ENGKLSSD  KEVESGAEKSILSQ KKQETQK
Sbjct: 421  GDSLSIKSLPGSFSDSEDSDQPYYLSDSENGKLSSDFAKEVESGAEKSILSQTKKQETQK 480

Query: 481  DLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVG 540
            DLTREGSPLN+PKALLKKSSRFFSASFFSFT DGTEFTPA VFQGLLDSTKKQLPKL+VG
Sbjct: 481  DLTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALVFQGLLDSTKKQLPKLIVG 540

Query: 541  LVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKL 600
             VLLG GI IFA RAER+ QM QQPDV+TTSTDEVSLNTKPL QQLRKLPKRVK+LIS+L
Sbjct: 541  AVLLGAGIAIFANRAERSSQMIQQPDVVTTSTDEVSLNTKPLLQQLRKLPKRVKKLISQL 600

Query: 601  PHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660
            PHQE   +EASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH
Sbjct: 601  PHQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660

Query: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPG 720
            GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ G
Sbjct: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAG 720

Query: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780
            PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN
Sbjct: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780

Query: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840
            SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV
Sbjct: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840

Query: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900
            ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP
Sbjct: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900

Query: 901  KLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEA 960
            KLLLSNFPVI GSLGLLIGGKTILVALVGR FGISIISA+RVGLLLAPGGEFAFVAFGEA
Sbjct: 901  KLLLSNFPVIMGSLGLLIGGKTILVALVGRFFGISIISAIRVGLLLAPGGEFAFVAFGEA 960

Query: 961  VNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020
            VNQGIMSS LSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH
Sbjct: 961  VNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020

Query: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080
            IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA
Sbjct: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080

Query: 1081 ERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140
            ERACAAAITLD+PGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP
Sbjct: 1081 ERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140

Query: 1141 SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT 1200
            SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT
Sbjct: 1141 SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT 1200

Query: 1201 SDSSDENQVTEGTLAI 1214
            SDSSDENQVTEGTLAI
Sbjct: 1201 SDSSDENQVTEGTLAI 1216

BLAST of MC05g0336 vs. NCBI nr
Match: KAG6582600.1 (K(+) efflux antiporter 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2015 bits (5220), Expect = 0.0
Identity = 1106/1216 (90.95%), Postives = 1143/1216 (94.00%), Query Frame = 0

Query: 1    MDLSCNIWKQNVF--SERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFI 60
            MDLSC+IWKQNV   SERT+CKTLDQFG   VLRSR +DAKL GGSRV+YK+P KRNR +
Sbjct: 1    MDLSCHIWKQNVLCGSERTHCKTLDQFGLRYVLRSRALDAKLSGGSRVIYKMPNKRNRIV 60

Query: 61   ASSNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNG 120
            +SS+S +LSLVC  KFD  LQI KHKRF NC    NGR+MGMVHSECQ NDSLAFIDGNG
Sbjct: 61   SSSDSIHLSLVCGSKFDGALQISKHKRFLNCN--YNGRKMGMVHSECQSNDSLAFIDGNG 120

Query: 121  RNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVA 180
            RN+EYVD GDE SSS PTDGVGS GSREAGGE EA ET APTVDELRELLQKA+KELEVA
Sbjct: 121  RNMEYVDTGDEGSSSVPTDGVGSAGSREAGGEAEAVETVAPTVDELRELLQKAIKELEVA 180

Query: 181  RLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240
            RLNSTMFEEKAQKISE AIALQDEA HA NDVNSTLDSVQQIV EEYAAKEAVQKATMAL
Sbjct: 181  RLNSTMFEEKAQKISETAIALQDEATHARNDVNSTLDSVQQIVKEEYAAKEAVQKATMAL 240

Query: 241  SLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENC 300
            SLAEARLQVAIES+E  KGGND PET+I SE EID K+E+EVLL+A+EDIKECR NLENC
Sbjct: 241  SLAEARLQVAIESMELAKGGNDFPETSINSEGEIDGKDEQEVLLVAQEDIKECRANLENC 300

Query: 301  DSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360
            D+ELKRLQS KEELQKEVDRLNELAE AQLNALKAEEDVANIMLLAEQAVAFELEAAQRV
Sbjct: 301  DAELKRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360

Query: 361  SDAEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFS 420
            SDAEIALQK+EKS S+SFVD SE TQGLNVIEEV NE+NKAV  ISGDIAVEMDRD+  S
Sbjct: 361  SDAEIALQKVEKSLSSSFVDTSEITQGLNVIEEVENEDNKAVPEISGDIAVEMDRDLPLS 420

Query: 421  ADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQK 480
             DSL +KSLPG+FSDSEDSDQPYYLSD+ENGKLSSDS KEVESGAEKSILSQAKK ETQK
Sbjct: 421  GDSLVIKSLPGSFSDSEDSDQPYYLSDSENGKLSSDSSKEVESGAEKSILSQAKKPETQK 480

Query: 481  DLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVG 540
            DLTREG P+N+PKALLKKSSRFFSASFFSF  DGTEFTPA VFQG LDSTKKQLPKLVVG
Sbjct: 481  DLTREGPPVNSPKALLKKSSRFFSASFFSFAVDGTEFTPALVFQGFLDSTKKQLPKLVVG 540

Query: 541  LVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKL 600
             VL G GI+I A RAERN  MFQQPDV+TTSTDEVSLNTKPLFQQLRKLPKRVK+LI+KL
Sbjct: 541  AVLFGAGISILANRAERNSLMFQQPDVVTTSTDEVSLNTKPLFQQLRKLPKRVKKLIAKL 600

Query: 601  PHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660
            PHQE   +EASLLD+LWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH
Sbjct: 601  PHQEVNEEEASLLDVLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660

Query: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPG 720
            GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ G
Sbjct: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAG 720

Query: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780
            PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN
Sbjct: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780

Query: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840
            SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV
Sbjct: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840

Query: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900
            ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP
Sbjct: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900

Query: 901  KLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEA 960
            KLLLSNFPVI GSLGLLIGGKTILVALVGRLFG+SIISALRVGLLLAPGGEFAFVAFGEA
Sbjct: 901  KLLLSNFPVIMGSLGLLIGGKTILVALVGRLFGVSIISALRVGLLLAPGGEFAFVAFGEA 960

Query: 961  VNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020
            VNQGIMSS LSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH
Sbjct: 961  VNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020

Query: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080
            IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA
Sbjct: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080

Query: 1081 ERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140
            ERACAAAITLD+PGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP
Sbjct: 1081 ERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140

Query: 1141 SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT 1200
            SLQLAAAVLAQAKLP SEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPK+QT
Sbjct: 1141 SLQLAAAVLAQAKLPTSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKVQT 1200

Query: 1201 SDSSDENQVTEGTLAI 1214
            SDSSDENQVTEGTLAI
Sbjct: 1201 SDSSDENQVTEGTLAI 1214

BLAST of MC05g0336 vs. ExPASy TrEMBL
Match: A0A6J1D1F3 (K(+) efflux antiporter 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016391 PE=4 SV=1)

HSP 1 Score: 2231 bits (5781), Expect = 0.0
Identity = 1210/1214 (99.67%), Postives = 1211/1214 (99.75%), Query Frame = 0

Query: 1    MDLSCNIWKQNVFSERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFIAS 60
            MDLSCNIWKQNVFSERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFIAS
Sbjct: 1    MDLSCNIWKQNVFSERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFIAS 60

Query: 61   SNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNGRN 120
            SNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNGRN
Sbjct: 61   SNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNGRN 120

Query: 121  VEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVARL 180
            VEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVARL
Sbjct: 121  VEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVARL 180

Query: 181  NSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSL 240
            NSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSL
Sbjct: 181  NSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMALSL 240

Query: 241  AEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENCDS 300
            AEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENCDS
Sbjct: 241  AEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENCDS 300

Query: 301  ELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSD 360
            ELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSD
Sbjct: 301  ELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVSD 360

Query: 361  AEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFSAD 420
            AEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFSAD
Sbjct: 361  AEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFSAD 420

Query: 421  SLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQKDL 480
            SLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQKDL
Sbjct: 421  SLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQKDL 480

Query: 481  TREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVGLV 540
            TREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVGLV
Sbjct: 481  TREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVGLV 540

Query: 541  LLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKLPH 600
            LLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKLPH
Sbjct: 541  LLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKLPH 600

Query: 601  QEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGT 660
            QE   +EASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGT
Sbjct: 601  QEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGT 660

Query: 661  KAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPA 720
            KAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPA
Sbjct: 661  KAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPGPA 720

Query: 721  AIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSS 780
            AIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSS
Sbjct: 721  AIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPNSS 780

Query: 781  KGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVIL 840
            KGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVIL
Sbjct: 781  KGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLVIL 840

Query: 841  GTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKL 900
            GTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKL
Sbjct: 841  GTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKL 900

Query: 901  LLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVN 960
            LLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVN
Sbjct: 901  LLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEAVN 960

Query: 961  QGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHII 1020
            QGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHII
Sbjct: 961  QGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDHII 1020

Query: 1021 ICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAER 1080
            ICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAER
Sbjct: 1021 ICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGAER 1080

Query: 1081 ACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSL 1140
            ACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSL
Sbjct: 1081 ACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSL 1140

Query: 1141 QLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSD 1200
            QLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSD
Sbjct: 1141 QLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQTSD 1200

Query: 1201 SSDENQVTEGTLAI 1214
            SSDENQVTEGTLAI
Sbjct: 1201 SSDENQVTEGTLAI 1214

BLAST of MC05g0336 vs. ExPASy TrEMBL
Match: A0A6J1IYK3 (K(+) efflux antiporter 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479651 PE=4 SV=1)

HSP 1 Score: 2024 bits (5244), Expect = 0.0
Identity = 1110/1216 (91.28%), Postives = 1146/1216 (94.24%), Query Frame = 0

Query: 1    MDLSCNIWKQNVF--SERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFI 60
            MDLSCNIWKQNV   SERT+CKTLDQFG   VLRSR +DAK+ GGSRV+YKVP KRNR +
Sbjct: 1    MDLSCNIWKQNVLCGSERTHCKTLDQFGLRYVLRSRALDAKMSGGSRVIYKVPNKRNRIV 60

Query: 61   ASSNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNG 120
            +SS+S +LSLVCA KFD  LQI KHKRF NC    NGR+MGMVHSECQ NDSLAFIDGNG
Sbjct: 61   SSSDSFHLSLVCASKFDGALQISKHKRFLNCN--YNGRKMGMVHSECQSNDSLAFIDGNG 120

Query: 121  RNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVA 180
            RN+EYVD GDE SSS PTDGVGS GSREAGGE EA ET APTVDELRELLQKAMKELEVA
Sbjct: 121  RNMEYVDTGDEGSSSVPTDGVGSAGSREAGGETEAVETVAPTVDELRELLQKAMKELEVA 180

Query: 181  RLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240
            RLNSTMFEEKAQKISE AIALQDEA HA NDVNSTLDSVQQIVNEEYAAKEAVQKATMAL
Sbjct: 181  RLNSTMFEEKAQKISETAIALQDEATHARNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240

Query: 241  SLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENC 300
            SLAEARLQVAIES+E  KGGND PET+I SEREID K+E++VLL+A+EDIKECR NLENC
Sbjct: 241  SLAEARLQVAIESMELAKGGNDFPETSINSEREIDGKDEQDVLLVAQEDIKECRANLENC 300

Query: 301  DSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360
            D+ELKRLQS KEELQKEVDRLNELAE AQLNALKAEEDVANIMLLAEQAVAFELEAAQRV
Sbjct: 301  DAELKRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360

Query: 361  SDAEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFS 420
            SDAEIALQK+EKS S+SFVD SE TQGLNVIEEV NE+NKAV  ISGDIAVEMDRD+  S
Sbjct: 361  SDAEIALQKVEKSLSSSFVDTSEITQGLNVIEEVENEDNKAVPEISGDIAVEMDRDLPLS 420

Query: 421  ADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQK 480
             DSL +KSLPG+FSDSEDSDQPYYLSD+ENGKLSSDS KEVESGAEKSILSQAKK ETQK
Sbjct: 421  GDSLVIKSLPGSFSDSEDSDQPYYLSDSENGKLSSDSSKEVESGAEKSILSQAKKPETQK 480

Query: 481  DLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVG 540
            DLTREGSP+N+PKALLKKSSRFFSASFFSF  DGTEFTPA VFQG LDSTKKQLPKLVVG
Sbjct: 481  DLTREGSPVNSPKALLKKSSRFFSASFFSFAVDGTEFTPALVFQGFLDSTKKQLPKLVVG 540

Query: 541  LVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKL 600
             VL G GI I A RAERN  MFQQPDV+TTSTDEVSLNTKPLFQQLRKLPKRVK+LI+KL
Sbjct: 541  AVLFGAGIAILANRAERNSLMFQQPDVVTTSTDEVSLNTKPLFQQLRKLPKRVKKLIAKL 600

Query: 601  PHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660
            PHQE   +EASLLD+LWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH
Sbjct: 601  PHQEVNEEEASLLDVLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660

Query: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPG 720
            GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ G
Sbjct: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAG 720

Query: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780
            PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN
Sbjct: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780

Query: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840
            SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV
Sbjct: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840

Query: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900
            ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP
Sbjct: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900

Query: 901  KLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEA 960
            KLLLSNFPVI GSLGLLIGGKTILVALVGRLFG+SIISALRVGLLLAPGGEFAFVAFGEA
Sbjct: 901  KLLLSNFPVIMGSLGLLIGGKTILVALVGRLFGVSIISALRVGLLLAPGGEFAFVAFGEA 960

Query: 961  VNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020
            VNQGIMSS LSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH
Sbjct: 961  VNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020

Query: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080
            IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA
Sbjct: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080

Query: 1081 ERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140
            ERACAAAITLD+PGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP
Sbjct: 1081 ERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140

Query: 1141 SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT 1200
            SLQLAAAVLAQAKLP SEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPK+QT
Sbjct: 1141 SLQLAAAVLAQAKLPTSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKVQT 1200

Query: 1201 SDSSDENQVTEGTLAI 1214
            SDSSDENQV+EGTLAI
Sbjct: 1201 SDSSDENQVSEGTLAI 1214

BLAST of MC05g0336 vs. ExPASy TrEMBL
Match: A0A6J1E9G2 (K(+) efflux antiporter 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111431943 PE=4 SV=1)

HSP 1 Score: 2013 bits (5215), Expect = 0.0
Identity = 1105/1216 (90.87%), Postives = 1141/1216 (93.83%), Query Frame = 0

Query: 1    MDLSCNIWKQNVF--SERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFI 60
            MDLSC+IWKQNV   SERT+CKTLDQFG   VLRSR +DAKL GGSRV+YKVP  RNR +
Sbjct: 1    MDLSCHIWKQNVLCGSERTHCKTLDQFGLRYVLRSRALDAKLSGGSRVIYKVPNNRNRIV 60

Query: 61   ASSNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNG 120
            +SS+S +LSLVC  KFD  LQI KHKRF NC    NGR+MGMVHSECQ NDSLAFIDGNG
Sbjct: 61   SSSDSIHLSLVCGSKFDGALQISKHKRFLNCN--YNGRKMGMVHSECQSNDSLAFIDGNG 120

Query: 121  RNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVA 180
            RN+EYVD GDE SSS PTDGVGS GSREAGGE EA ET APTVDELRELLQKA+KELEVA
Sbjct: 121  RNMEYVDTGDEGSSSVPTDGVGSAGSREAGGEAEAVETVAPTVDELRELLQKAIKELEVA 180

Query: 181  RLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240
            RLNSTMFEEKAQKISE AIALQDEA HA NDVNSTLDSVQQIV EEYAAKEAVQKATMAL
Sbjct: 181  RLNSTMFEEKAQKISETAIALQDEATHARNDVNSTLDSVQQIVKEEYAAKEAVQKATMAL 240

Query: 241  SLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENC 300
            SLAEARLQVAIES+E  KGGND PET+I SE EID K+E+EVLL+A+EDIKECR NLENC
Sbjct: 241  SLAEARLQVAIESMELAKGGNDFPETSINSEGEIDGKDEQEVLLVAQEDIKECRANLENC 300

Query: 301  DSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360
            D+ELKRLQS KEELQKEVDRLNELAE AQLNALKAEEDVANIMLLAEQAVAFELEAAQRV
Sbjct: 301  DAELKRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360

Query: 361  SDAEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFS 420
            SDAEIALQK+EKS S+SFVD SE TQGLNVIEEV NE+NKAV  ISGDIAVEMDRD+  S
Sbjct: 361  SDAEIALQKVEKSLSSSFVDTSEITQGLNVIEEVENEDNKAVPEISGDIAVEMDRDLPLS 420

Query: 421  ADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQK 480
             DSL +KSLPG+FSDSEDSDQPYYLSD+ENG+LSSDS KEVESGAEKSILSQAKK ETQK
Sbjct: 421  GDSLVIKSLPGSFSDSEDSDQPYYLSDSENGRLSSDSSKEVESGAEKSILSQAKKPETQK 480

Query: 481  DLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVG 540
            DLTREGSP+N+PKALLKKSSRFFSASFFSF  DGTEFTPA VFQG LDSTKKQLPKLVVG
Sbjct: 481  DLTREGSPVNSPKALLKKSSRFFSASFFSFAVDGTEFTPALVFQGFLDSTKKQLPKLVVG 540

Query: 541  LVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKL 600
             VL G GI I   RAERN  MFQQPDV+TTSTDEVSLNTKPLFQQLRKLPKRVK+LI+KL
Sbjct: 541  AVLFGAGIAILGNRAERNSLMFQQPDVVTTSTDEVSLNTKPLFQQLRKLPKRVKKLIAKL 600

Query: 601  PHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660
            PHQE   +EASLLD+LWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH
Sbjct: 601  PHQEVNEEEASLLDVLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660

Query: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPG 720
            GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQ G
Sbjct: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQAG 720

Query: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780
            PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN
Sbjct: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780

Query: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840
            SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV
Sbjct: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840

Query: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900
            ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP
Sbjct: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900

Query: 901  KLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEA 960
            KLLLSNFPVI GSLGLLIGGKTILVALVGRLFG+SIISALRVGLLLAPGGEFAFVAFGEA
Sbjct: 901  KLLLSNFPVIMGSLGLLIGGKTILVALVGRLFGVSIISALRVGLLLAPGGEFAFVAFGEA 960

Query: 961  VNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020
            VNQGIMSS LSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH
Sbjct: 961  VNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020

Query: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080
            IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA
Sbjct: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080

Query: 1081 ERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140
            ERACAAAITLD+PGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP
Sbjct: 1081 ERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140

Query: 1141 SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT 1200
            SLQLAAAVLAQAKLP SEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPK+QT
Sbjct: 1141 SLQLAAAVLAQAKLPTSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKVQT 1200

Query: 1201 SDSSDENQVTEGTLAI 1214
            SDSSDENQVTEGTLAI
Sbjct: 1201 SDSSDENQVTEGTLAI 1214

BLAST of MC05g0336 vs. ExPASy TrEMBL
Match: A0A5A7U5W1 (K(+) efflux antiporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002050 PE=4 SV=1)

HSP 1 Score: 1991 bits (5159), Expect = 0.0
Identity = 1090/1221 (89.27%), Postives = 1141/1221 (93.45%), Query Frame = 0

Query: 1    MDLSCNIWKQNVF--SERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFI 60
            MDLSC+ WKQNV   SERTYCKTLDQFG  +VLR+RI DAKL GGSRV YKVPKKRNR +
Sbjct: 43   MDLSCSTWKQNVLRGSERTYCKTLDQFGPRSVLRNRIFDAKLTGGSRVSYKVPKKRNRIV 102

Query: 61   ASSNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNG 120
            ASS+SN+LSLVCA KFD  LQIF  KRF N  NKI+GR MGMVH ECQ NDSLAFIDGNG
Sbjct: 103  ASSDSNHLSLVCASKFDRALQIFTPKRFLNWNNKISGRGMGMVHLECQNNDSLAFIDGNG 162

Query: 121  RNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVA 180
            RN+EYV++GDE SSSGP DGVGS GSRE GGE E  ET+ PTVDELRELLQKAMKELEVA
Sbjct: 163  RNIEYVNSGDEGSSSGPADGVGSAGSREVGGEAETVETNIPTVDELRELLQKAMKELEVA 222

Query: 181  RLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240
            RLNSTMFEE+AQKISEAAIALQDEA +AWNDVNSTLDSVQQIVNEEY AKEAVQKATMAL
Sbjct: 223  RLNSTMFEERAQKISEAAIALQDEATNAWNDVNSTLDSVQQIVNEEYVAKEAVQKATMAL 282

Query: 241  SLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENC 300
            SLAEARLQVAIESLE  K G+D PET++ S+  ID KE++E LL+A+EDI +CR NLE C
Sbjct: 283  SLAEARLQVAIESLELAKRGSDFPETSMDSDGVIDGKEDQEALLVAQEDITDCRANLEIC 342

Query: 301  DSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360
            ++EL RLQS KEELQKEVDRLNELAE AQLNALKAEEDVANIMLLAEQAVAFELEAAQRV
Sbjct: 343  NAELMRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 402

Query: 361  SDAEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFS 420
            +DAE ALQK+EKS S+SFVD S+ TQG NVIEEV NE+NKAV+ ISGDIAVEMDR++  +
Sbjct: 403  NDAERALQKVEKSLSSSFVDTSDITQGSNVIEEVENEDNKAVLEISGDIAVEMDRELPLN 462

Query: 421  ADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQK 480
             DSLA+KSLPG+ SDSE SDQPYYLSD+ENGKLSSDS KEVESGAEKSILSQ KKQE QK
Sbjct: 463  GDSLAIKSLPGSLSDSEGSDQPYYLSDSENGKLSSDSAKEVESGAEKSILSQTKKQEIQK 522

Query: 481  DLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVG 540
            DLTREGSPLN+PKALLKKSSRFFSASFFSFT DGTEFTPA VFQGLLDSTKKQLPKL+VG
Sbjct: 523  DLTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALVFQGLLDSTKKQLPKLIVG 582

Query: 541  LVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKL 600
             VLLG GI +FA RAER+ QM  QPDV+T STD+VSL+TKPLFQQLRKLPKRVK+LIS++
Sbjct: 583  AVLLGAGIAVFANRAERSSQMIPQPDVVTISTDDVSLDTKPLFQQLRKLPKRVKKLISQI 642

Query: 601  PHQEACS-----QEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSI 660
            PHQEAC+     +EASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSI
Sbjct: 643  PHQEACTLLVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSI 702

Query: 661  IRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIV 720
            IRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAH+V
Sbjct: 703  IRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHMV 762

Query: 721  CGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIP 780
            CGQ GPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIP
Sbjct: 763  CGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIP 822

Query: 781  LISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSA 840
            LISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSA
Sbjct: 823  LISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSA 882

Query: 841  NTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVG 900
            NTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVG
Sbjct: 883  NTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVG 942

Query: 901  MSIDPKLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFV 960
            MSIDPKLL SNFPVI GSLGLLIGGKTILVALVGRLFGISIISA+RVGLLLAPGGEFAFV
Sbjct: 943  MSIDPKLLFSNFPVIMGSLGLLIGGKTILVALVGRLFGISIISAIRVGLLLAPGGEFAFV 1002

Query: 961  AFGEAVNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETD 1020
            AFGEAVNQGIMSS LSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETD
Sbjct: 1003 AFGEAVNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETD 1062

Query: 1021 DLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVL 1080
            DLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVL
Sbjct: 1063 DLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVL 1122

Query: 1081 HKVGAERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVP 1140
            HKVGAERACAAAITLD+PGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVP
Sbjct: 1123 HKVGAERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVP 1182

Query: 1141 ETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSK 1200
            ETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSK
Sbjct: 1183 ETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSK 1242

Query: 1201 PKIQTSDSSDENQVTEGTLAI 1214
            PKIQTSDSSDENQVTEGTLAI
Sbjct: 1243 PKIQTSDSSDENQVTEGTLAI 1263

BLAST of MC05g0336 vs. ExPASy TrEMBL
Match: A0A5D3D075 (K(+) efflux antiporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004530 PE=4 SV=1)

HSP 1 Score: 1991 bits (5158), Expect = 0.0
Identity = 1088/1216 (89.47%), Postives = 1138/1216 (93.59%), Query Frame = 0

Query: 1    MDLSCNIWKQNVF--SERTYCKTLDQFGHSNVLRSRIIDAKLIGGSRVMYKVPKKRNRFI 60
            MDLSC+ WKQNV   SERTYCKTLDQFG  +VLR+RI DAKL GGSRV YKVPKKRNR +
Sbjct: 1    MDLSCSTWKQNVLRGSERTYCKTLDQFGPRSVLRNRIFDAKLTGGSRVSYKVPKKRNRIV 60

Query: 61   ASSNSNNLSLVCARKFDSVLQIFKHKRFFNCTNKINGRRMGMVHSECQGNDSLAFIDGNG 120
            ASS+SN+LSLVCA KFD  LQIF  KRF N  NKI+GR MGMVH ECQ NDSLAFIDGNG
Sbjct: 61   ASSDSNHLSLVCASKFDRALQIFTPKRFLNWNNKISGRGMGMVHLECQNNDSLAFIDGNG 120

Query: 121  RNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDELRELLQKAMKELEVA 180
            RN+EYV++GDE SSSGP DGVGS GSRE GGE E  ET+ PTVDELRELLQKAMKELEVA
Sbjct: 121  RNIEYVNSGDEGSSSGPADGVGSAGSREVGGEAETVETNIPTVDELRELLQKAMKELEVA 180

Query: 181  RLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEEYAAKEAVQKATMAL 240
            RLNSTMFEE+AQKISEAAIALQDEA +AWNDVNSTLDSVQQIVNEEY AKEAVQKATMAL
Sbjct: 181  RLNSTMFEERAQKISEAAIALQDEATNAWNDVNSTLDSVQQIVNEEYVAKEAVQKATMAL 240

Query: 241  SLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIAKEDIKECRENLENC 300
            SLAEARLQVAIESLE  K G+D PET++ S+  ID KE++E LL+A+EDI +CR NLE C
Sbjct: 241  SLAEARLQVAIESLELAKRGSDFPETSMDSDGVIDGKEDQEALLVAQEDITDCRANLEIC 300

Query: 301  DSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360
            ++EL RLQS KEELQKEVDRLNELAE AQLNALKAEEDVANIMLLAEQAVAFELEAAQRV
Sbjct: 301  NAELMRLQSKKEELQKEVDRLNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRV 360

Query: 361  SDAEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGISGDIAVEMDRDVAFS 420
            +DAE ALQK+EKS S+SFVD S+ TQG NVIEEV NE+NKAV+ ISGDIAVEMDR++  +
Sbjct: 361  NDAERALQKVEKSLSSSFVDTSDITQGSNVIEEVENEDNKAVLEISGDIAVEMDRELPLN 420

Query: 421  ADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVESGAEKSILSQAKKQETQK 480
             DSLA+KSLPG+ SDSE SDQPYYLSD+ENGKLSSDS KEVESGAEKSILSQ KKQE QK
Sbjct: 421  GDSLAIKSLPGSLSDSEGSDQPYYLSDSENGKLSSDSAKEVESGAEKSILSQTKKQEIQK 480

Query: 481  DLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASVFQGLLDSTKKQLPKLVVG 540
            DLTREGSPLN+PKALLKKSSRFFSASFFSFT DGTEFTPA VFQGLLDSTKKQLPKL+VG
Sbjct: 481  DLTREGSPLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALVFQGLLDSTKKQLPKLIVG 540

Query: 541  LVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPLFQQLRKLPKRVKELISKL 600
             VLLG GI +FA RAER+ QM  QPDV+T STD+VSL+TKPLFQQLRKLPKRVK+LIS++
Sbjct: 541  AVLLGAGIAVFANRAERSSQMIPQPDVVTISTDDVSLDTKPLFQQLRKLPKRVKKLISQI 600

Query: 601  PHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660
            PHQE   +EASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH
Sbjct: 601  PHQEVNEEEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVH 660

Query: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHIVCGQPG 720
            GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAH+VCGQ G
Sbjct: 661  GTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAVVVGLVAHMVCGQAG 720

Query: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780
            PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN
Sbjct: 721  PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLILIPLISPN 780

Query: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840
            SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV
Sbjct: 781  SSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIFSANTLLV 840

Query: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900
            ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP
Sbjct: 841  ILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDP 900

Query: 901  KLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFAFVAFGEA 960
            KLL SNFPVI GSLGLLIGGKTILVALVGRLFGISIISA+RVGLLLAPGGEFAFVAFGEA
Sbjct: 901  KLLFSNFPVIMGSLGLLIGGKTILVALVGRLFGISIISAIRVGLLLAPGGEFAFVAFGEA 960

Query: 961  VNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020
            VNQGIMSS LSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH
Sbjct: 961  VNQGIMSSQLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESETDDLQDH 1020

Query: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080
            IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA
Sbjct: 1021 IIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDLPVYFGDAGSREVLHKVGA 1080

Query: 1081 ERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140
            ERACAAAITLD+PGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP
Sbjct: 1081 ERACAAAITLDTPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEP 1140

Query: 1141 SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT 1200
            SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT
Sbjct: 1141 SLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSLGYGFSRIMSKPKIQT 1200

Query: 1201 SDSSDENQVTEGTLAI 1214
            SDSSDENQVTEGTLAI
Sbjct: 1201 SDSSDENQVTEGTLAI 1216

BLAST of MC05g0336 vs. TAIR 10
Match: AT4G00630.1 (K+ efflux antiporter 2 )

HSP 1 Score: 1439.9 bits (3726), Expect = 0.0e+00
Identity = 828/1115 (74.26%), Postives = 928/1115 (83.23%), Query Frame = 0

Query: 105  CQGNDSLAFIDGNGRNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETD-APTVDE 164
            CQ +DS+  + GN RN+E+ +  D+   +          S+E   E +  E D AP+++E
Sbjct: 98   CQSSDSVGDLVGNDRNLEFAEGSDDREVT---------FSKE---EKDTREQDSAPSLEE 157

Query: 165  LRELLQKAMKELEVARLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNE 224
            LR+LL KA KELEVA LNSTMFEEKAQ+ISE AIAL+DEAA AWNDVN TL+ VQ+ V+E
Sbjct: 158  LRDLLNKATKELEVASLNSTMFEEKAQRISEVAIALKDEAASAWNDVNQTLNVVQEAVDE 217

Query: 225  EYAAKEAVQKATMALSLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLI 284
            E  AKEAVQKATMALSLAEARLQVA+ESLE E G N S E    SE     K++EE LL 
Sbjct: 218  ESVAKEAVQKATMALSLAEARLQVALESLEAE-GYNTSEE----SEVRDGVKDKEEALLS 277

Query: 285  AKEDIKECRENLENCDSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLL 344
            AK DIKEC+ENL +C+ +L+RLQ  K+ELQKEVDRLNE AE AQ++ALKAEEDVANIM+L
Sbjct: 278  AKADIKECQENLASCEEQLRRLQVKKDELQKEVDRLNEAAERAQISALKAEEDVANIMVL 337

Query: 345  AEQAVAFELEAAQRVSDAEIALQKLEKSSSNSFVDVSETTQG--LNVIEEVVNEENKAVI 404
            AEQAVAFELEA QRV+DAEIALQ+ EK+   S     ETTQG  L+    +V E+   V+
Sbjct: 338  AEQAVAFELEATQRVNDAEIALQRAEKTLFGS--QTQETTQGKVLDGKNTIVGEDE--VL 397

Query: 405  GISGDIAVEMDRDVAFSADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVES 464
                D++ + +RD+      + V S  GT S         Y SDNENGK ++D  KE E 
Sbjct: 398  SEIVDVSHQAERDLVV----VGVSSDVGTQS---------YESDNENGKPTADFAKEAEG 457

Query: 465  GAEKS-ILSQAKKQETQKDLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASV 524
             AEKS  +   KKQE QKDL RE S  N  K  LKKSSRFF ASFFS  GDGT    A+V
Sbjct: 458  EAEKSKNVVLTKKQEVQKDLPRESSSHNGTKTSLKKSSRFFPASFFSSNGDGT----ATV 517

Query: 525  FQGLLDSTKKQLPKLVVGLVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPL 584
            F+ L++S K+Q PKL++G  LLG G+ I++    RN Q+ QQP++++TS ++VS +TKPL
Sbjct: 518  FESLVESAKQQWPKLILGFTLLGAGVAIYSNGVGRNNQLPQQPNIVSTSAEDVSSSTKPL 577

Query: 585  FQQLRKLPKRVKELISKLPHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYL 644
             +Q++KLPKR+K+L+   P QE   +EASLLD+LWLLLASVIFVP FQK+PGGSPVLGYL
Sbjct: 578  IRQMQKLPKRIKKLLEMFPQQEVNEEEASLLDVLWLLLASVIFVPLFQKIPGGSPVLGYL 637

Query: 645  AAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVL 704
            AAGILIGPYGLSIIR+VHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVL
Sbjct: 638  AAGILIGPYGLSIIRNVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVL 697

Query: 705  VTAVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLF 764
            VTA V+GL+ H V GQ GPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLF
Sbjct: 698  VTAAVIGLITHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLF 757

Query: 765  QDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYK 824
            QDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAA+KA VAI+ IIAGGRLLLRPIYK
Sbjct: 758  QDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLLLRPIYK 817

Query: 825  QIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPY 884
            QIAEN+NAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPY
Sbjct: 818  QIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPY 877

Query: 885  RGLLLGLFFMTVGMSIDPKLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRV 944
            RGLLLGLFFMTVGMSIDPKLLL+NFP+I G+LGLL+ GKTILV ++G+LFGISIISA+RV
Sbjct: 878  RGLLLGLFFMTVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVRV 937

Query: 945  GLLLAPGGEFAFVAFGEAVNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQH 1004
            GLLLAPGGEFAFVAFGEAVNQGIM+  LSSLLFLVVGISMALTPWLAAGGQLIASRFE  
Sbjct: 938  GLLLAPGGEFAFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGGQLIASRFELQ 997

Query: 1005 DVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRALDL 1064
            DVRSLLPVESETDDLQ HIIICGFGR+GQIIAQLLSERLIPFVALDV SDRVA+GR+LDL
Sbjct: 998  DVRSLLPVESETDDLQGHIIICGFGRIGQIIAQLLSERLIPFVALDVSSDRVAIGRSLDL 1057

Query: 1065 PVYFGDAGSREVLHKVGAERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHG 1124
            PVYFGDAGSREVLHK+GA+RACAAAI LD+PGANYR VWALSKYFPNVKTFVRAHDVDHG
Sbjct: 1058 PVYFGDAGSREVLHKIGADRACAAAIALDTPGANYRCVWALSKYFPNVKTFVRAHDVDHG 1117

Query: 1125 LNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASG 1184
            LNLEKAGATAVVPETLEPSLQLAAAVLAQAKLP SEIA TINEFRSRHLSEL ELCEASG
Sbjct: 1118 LNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPTSEIATTINEFRSRHLSELAELCEASG 1174

Query: 1185 SSLGYGFSRIMSKPKIQT-SDSSDENQVTEGTLAI 1215
            SSLGYGFSR  SKPK  + S++SD+NQ+ EGTLAI
Sbjct: 1178 SSLGYGFSRSTSKPKPPSPSETSDDNQIIEGTLAI 1174

BLAST of MC05g0336 vs. TAIR 10
Match: AT1G01790.1 (K+ efflux antiporter 1 )

HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 819/1112 (73.65%), Postives = 916/1112 (82.37%), Query Frame = 0

Query: 105  CQGNDSLAFIDGNGRNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETDAPTVDEL 164
            CQG++SL   D N   +     G+ S SS  T+      +R         E D  +++EL
Sbjct: 98   CQGHESLGNADSNDHRI-----GESSESSDETEATDLKDAR--------VENDTDSLEEL 157

Query: 165  RELLQKAMKELEVARLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNEE 224
            +ELL KA+KELEVARLNSTMFEEKAQ+ISE AIAL+DEAA AW DVN TLD ++  V EE
Sbjct: 158  KELLHKAIKELEVARLNSTMFEEKAQRISERAIALKDEAATAWLDVNKTLDVIRDTVYEE 217

Query: 225  YAAKEAVQKATMALSLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLIA 284
              AKEAVQ ATMALSLAEARLQV +ESLE    GND P  +  +E  ID  ++EE LL A
Sbjct: 218  ALAKEAVQTATMALSLAEARLQVIVESLE-AGAGNDIPHVSEETEETIDVNDKEEALLAA 277

Query: 285  KEDIKECRENLENCDSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLLA 344
            K+DIKEC+ NL+NC+S+L  L S K+ELQKEVD+LNE AET Q+++LKAEEDV NIM LA
Sbjct: 278  KDDIKECQVNLDNCESQLSALLSKKDELQKEVDKLNEFAETIQISSLKAEEDVTNIMKLA 337

Query: 345  EQAVAFELEAAQRVSDAEIALQKLEKSSSNSFVDVSETTQGLNVIEEVVNEENKAVIGIS 404
            EQAVAFELEA QRV+DAEIALQ+ EKS S S     E TQG    EE   E+   + G  
Sbjct: 338  EQAVAFELEATQRVNDAEIALQRAEKSLSIS--QTPEETQGQLSDEETSQEDAMVLSGNV 397

Query: 405  GDIAVEMDRDVAFSADSLAVKSLPGTFSD-----SEDSDQPYYLSDNENGKLSSDSLKEV 464
             D+  +++++     D   V+       D     ++   QPY  SD+ENGK S +S K V
Sbjct: 398  EDVTHQVEKESPKDGDLPVVQITAELVPDIVGQRNKKLTQPYESSDHENGKPSVESSKVV 457

Query: 465  ESGAEK-SILSQAKKQETQKDLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPA 524
            E+ +EK  I  Q KKQETQKDL +EGS LN+PKA   KSSRFFSASFFS   DGT    A
Sbjct: 458  EADSEKPKINVQTKKQETQKDLPKEGSSLNSPKASFNKSSRFFSASFFSSNPDGT----A 517

Query: 525  SVFQGLLDSTKKQLPKLVVGLVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTK 584
            +VF  L+ S K+Q PKLV+GL LLG G+T+++     N Q+ QQPDV +TST++VS +TK
Sbjct: 518  TVFGSLVGSVKQQWPKLVLGLALLGAGLTLYSNGVGGNNQLLQQPDVTSTSTEDVSSSTK 577

Query: 585  PLFQQLRKLPKRVKELISKLPHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLG 644
            PL +Q++KLPKR+K+L+  +PHQE   +EASL D LWLLLASVIFVP FQK+PGGSPVLG
Sbjct: 578  PLIRQVQKLPKRIKKLLEMIPHQEVNEEEASLFDFLWLLLASVIFVPLFQKIPGGSPVLG 637

Query: 645  YLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQ 704
            YLAAGILIGPYGLSIIR+VHGT+AIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQ
Sbjct: 638  YLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQ 697

Query: 705  VLVTAVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVL 764
            VLVTA VVGL+AH V GQ GPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRA+FSVL
Sbjct: 698  VLVTAAVVGLLAHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRASFSVL 757

Query: 765  LFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPI 824
            LFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKA VAI+AIIAGGRLLLRPI
Sbjct: 758  LFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAAVAITAIIAGGRLLLRPI 817

Query: 825  YKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIA 884
            YKQIAEN+NAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIA
Sbjct: 818  YKQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIA 877

Query: 885  PYRGLLLGLFFMTVGMSIDPKLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISAL 944
            PYRGLLLGLFFMTVGMSIDPKLLLSNFPVI G+LGLLI GKT+LV ++G+LFGISIISA+
Sbjct: 878  PYRGLLLGLFFMTVGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMGKLFGISIISAI 937

Query: 945  RVGLLLAPGGEFAFVAFGEAVNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFE 1004
            RVGLLLAPGGEFAFVAFGEAVNQGIMS  LSSLLFLVVGISMA+TPWLAAGGQLIASRFE
Sbjct: 938  RVGLLLAPGGEFAFVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWLAAGGQLIASRFE 997

Query: 1005 QHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRSDRVAVGRAL 1064
             HDVRSLLPVESETDDLQ HIIICGFGRVGQIIAQLLSERLIPFVALDV SDRV +GR+L
Sbjct: 998  LHDVRSLLPVESETDDLQGHIIICGFGRVGQIIAQLLSERLIPFVALDVSSDRVTIGRSL 1057

Query: 1065 DLPVYFGDAGSREVLHKVGAERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVD 1124
            DLPVYFGDAGS+EVLHK+GA RACAA + LD+PGANYR VWALSK++PNVKTFVRAHDV 
Sbjct: 1058 DLPVYFGDAGSKEVLHKIGAGRACAAVVALDAPGANYRCVWALSKFYPNVKTFVRAHDVV 1117

Query: 1125 HGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEA 1184
            HGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLP SEIA TINEFR+RHLSELTELCEA
Sbjct: 1118 HGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPTSEIANTINEFRTRHLSELTELCEA 1177

Query: 1185 SGSSLGYGFSRIMSKPKIQTSDSSDENQVTEG 1211
            SGSSLGYG+SR  SKPK Q SD+S +NQ+ EG
Sbjct: 1178 SGSSLGYGYSR-TSKPKPQPSDASGDNQIIEG 1188

BLAST of MC05g0336 vs. TAIR 10
Match: AT4G00630.2 (K+ efflux antiporter 2 )

HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 828/1126 (73.53%), Postives = 928/1126 (82.42%), Query Frame = 0

Query: 105  CQGNDSLAFIDGNGRNVEYVDNGDESSSSGPTDGVGSDGSREAGGELEAAETD-APTVDE 164
            CQ +DS+  + GN RN+E+ +  D+   +          S+E   E +  E D AP+++E
Sbjct: 98   CQSSDSVGDLVGNDRNLEFAEGSDDREVT---------FSKE---EKDTREQDSAPSLEE 157

Query: 165  LRELLQKAMKELEVARLNSTMFEEKAQKISEAAIALQDEAAHAWNDVNSTLDSVQQIVNE 224
            LR+LL KA KELEVA LNSTMFEEKAQ+ISE AIAL+DEAA AWNDVN TL+ VQ+ V+E
Sbjct: 158  LRDLLNKATKELEVASLNSTMFEEKAQRISEVAIALKDEAASAWNDVNQTLNVVQEAVDE 217

Query: 225  EYAAKEAVQKATMALSLAEARLQVAIESLEQEKGGNDSPETAIVSEREIDCKEEEEVLLI 284
            E  AKEAVQKATMALSLAEARLQVA+ESLE E G N S E    SE     K++EE LL 
Sbjct: 218  ESVAKEAVQKATMALSLAEARLQVALESLEAE-GYNTSEE----SEVRDGVKDKEEALLS 277

Query: 285  AKEDIKECRENLENCDSELKRLQSIKEELQKEVDRLNELAETAQLNALKAEEDVANIMLL 344
            AK DIKEC+ENL +C+ +L+RLQ  K+ELQKEVDRLNE AE AQ++ALKAEEDVANIM+L
Sbjct: 278  AKADIKECQENLASCEEQLRRLQVKKDELQKEVDRLNEAAERAQISALKAEEDVANIMVL 337

Query: 345  AEQAVAFELEAAQRVSDAEIALQKLEKSSSNSFVDVSETTQG--LNVIEEVVNEENKAVI 404
            AEQAVAFELEA QRV+DAEIALQ+ EK+   S     ETTQG  L+    +V E+   V+
Sbjct: 338  AEQAVAFELEATQRVNDAEIALQRAEKTLFGS--QTQETTQGKVLDGKNTIVGEDE--VL 397

Query: 405  GISGDIAVEMDRDVAFSADSLAVKSLPGTFSDSEDSDQPYYLSDNENGKLSSDSLKEVES 464
                D++ + +RD+      + V S  GT S         Y SDNENGK ++D  KE E 
Sbjct: 398  SEIVDVSHQAERDLVV----VGVSSDVGTQS---------YESDNENGKPTADFAKEAEG 457

Query: 465  GAEKS-ILSQAKKQETQKDLTREGSPLNAPKALLKKSSRFFSASFFSFTGDGTEFTPASV 524
             AEKS  +   KKQE QKDL RE S  N  K  LKKSSRFF ASFFS  GDGT    A+V
Sbjct: 458  EAEKSKNVVLTKKQEVQKDLPRESSSHNGTKTSLKKSSRFFPASFFSSNGDGT----ATV 517

Query: 525  FQGLLDSTKKQLPKLVVGLVLLGTGITIFATRAERNYQMFQQPDVITTSTDEVSLNTKPL 584
            F+ L++S K+Q PKL++G  LLG G+ I++    RN Q+ QQP++++TS ++VS +TKPL
Sbjct: 518  FESLVESAKQQWPKLILGFTLLGAGVAIYSNGVGRNNQLPQQPNIVSTSAEDVSSSTKPL 577

Query: 585  FQQLRKLPKRVKELISKLPHQEACSQEASLLDMLWLLLASVIFVPTFQKLPGGSPVLGYL 644
             +Q++KLPKR+K+L+   P QE   +EASLLD+LWLLLASVIFVP FQK+PGGSPVLGYL
Sbjct: 578  IRQMQKLPKRIKKLLEMFPQQEVNEEEASLLDVLWLLLASVIFVPLFQKIPGGSPVLGYL 637

Query: 645  AAGILIGPYGLSIIRHVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVL 704
            AAGILIGPYGLSIIR+VHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVL
Sbjct: 638  AAGILIGPYGLSIIRNVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYVFGLGSAQVL 697

Query: 705  VTAVVVGLVAHIVCGQPGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLF 764
            VTA V+GL+ H V GQ GPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLF
Sbjct: 698  VTAAVIGLITHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLF 757

Query: 765  QDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRL------- 824
            QDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAA+KA VAI+ IIAGGRL       
Sbjct: 758  QDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLFYNALIF 817

Query: 825  ----LLRPIYKQIAENQNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEF 884
                LLRPIYKQIAEN+NAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEF
Sbjct: 818  WYTQLLRPIYKQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEF 877

Query: 885  SLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVISGSLGLLIGGKTILVALVGRL 944
            SLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLL+NFP+I G+LGLL+ GKTILV ++G+L
Sbjct: 878  SLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKL 937

Query: 945  FGISIISALRVGLLLAPGGEFAFVAFGEAVNQGIMSSHLSSLLFLVVGISMALTPWLAAG 1004
            FGISIISA+RVGLLLAPGGEFAFVAFGEAVNQGIM+  LSSLLFLVVGISMALTPWLAAG
Sbjct: 938  FGISIISAVRVGLLLAPGGEFAFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAG 997

Query: 1005 GQLIASRFEQHDVRSLLPVESETDDLQDHIIICGFGRVGQIIAQLLSERLIPFVALDVRS 1064
            GQLIASRFE  DVRSLLPVESETDDLQ HIIICGFGR+GQIIAQLLSERLIPFVALDV S
Sbjct: 998  GQLIASRFELQDVRSLLPVESETDDLQGHIIICGFGRIGQIIAQLLSERLIPFVALDVSS 1057

Query: 1065 DRVAVGRALDLPVYFGDAGSREVLHKVGAERACAAAITLDSPGANYRTVWALSKYFPNVK 1124
            DRVA+GR+LDLPVYFGDAGSREVLHK+GA+RACAAAI LD+PGANYR VWALSKYFPNVK
Sbjct: 1058 DRVAIGRSLDLPVYFGDAGSREVLHKIGADRACAAAIALDTPGANYRCVWALSKYFPNVK 1117

Query: 1125 TFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHL 1184
            TFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLP SEIA TINEFRSRHL
Sbjct: 1118 TFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVLAQAKLPTSEIATTINEFRSRHL 1177

Query: 1185 SELTELCEASGSSLGYGFSRIMSKPKIQT-SDSSDENQVTEGTLAI 1215
            SEL ELCEASGSSLGYGFSR  SKPK  + S++SD+NQ+ EGTLAI
Sbjct: 1178 SELAELCEASGSSLGYGFSRSTSKPKPPSPSETSDDNQIIEGTLAI 1185

BLAST of MC05g0336 vs. TAIR 10
Match: AT4G04850.2 (K+ efflux antiporter 3 )

HSP 1 Score: 335.5 bits (859), Expect = 1.8e-91
Identity = 223/601 (37.10%), Postives = 332/601 (55.24%), Query Frame = 0

Query: 612  DMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFL 671
            D L  L+ +VI VP F+ L   SP+LG+  AG+++  +GL  IR++   K ++E+G++FL
Sbjct: 107  DTLTFLMVTVIIVPAFRILK-ASPILGFFFAGVVLNQFGL--IRNLTDVKVLSEWGILFL 166

Query: 672  LFNIGLELSVERLSSMKKYVFGLGSAQVLVT-------------AVVVGLVAHIVCGQPG 731
            LF +GLELS+ RL ++ K+ FG+G  QVL+              A+   ++  +   +P 
Sbjct: 167  LFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHSRPD 226

Query: 732  -------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLIL 791
                     A+VIG  L+LSS+A VLQ+L E+GE  +R G AT  +LL QD+AVV LL++
Sbjct: 227  LVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVI 286

Query: 792  IPLISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIF 851
            +P++        IG ++I   L   + KA+  +  +  GG+  LR I++ +AE +++E F
Sbjct: 287  LPVLESQD----IGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAF 346

Query: 852  SANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMT 911
             A  LL + GTSL+T   G S  LGAFLAG LLAET F  Q+E+DI P+RGLLLGLFF+T
Sbjct: 347  VALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVT 406

Query: 912  VGMSIDPKLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFA 971
             G SID ++L   +P +   LG LI  KT+++  +G   G++I  ++RVG LL+ GGEFA
Sbjct: 407  TGTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFA 466

Query: 972  FVAFGEAVNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESE 1031
            FV F  A   G++ + L+ LL +VV +SMALTP+L   G+  A   ++         E  
Sbjct: 467  FVVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDV 526

Query: 1032 TDDLQDHIIICGFGRVGQIIAQLLSERLI--------PFVALDVRSDRVAVGRALDLPVY 1091
              D+ + I+I GFG++GQ++A  LS  L+        P++  D+    V   R L  P+ 
Sbjct: 527  NFDVSESIVIIGFGQMGQVLANFLSTPLVSDSDLVGWPYIGFDLNPAVVKESRKLGFPIL 586

Query: 1092 FGDAGSREVLHKVGAERACAAAITLDSPGANYRTVWALSKYFPNVKTFVRAHDVDHGLNL 1151
            +GD     VL   G     A  I           V  L   FP    + RA D+ H L L
Sbjct: 587  YGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGSPIYARAQDLPHLLEL 646

Query: 1152 EKAGATAVVPETLEPSLQLAAAVLAQAKLPMSEIAATINEFRSRHLSELTELCEASGSSL 1185
            +KAGAT  + E  E SLQL + +L    +   +++     FR     +  E   AS ++ 
Sbjct: 647  KKAGATDAILENAETSLQLGSKLLTGFGVMSDDVSFLSKVFRDSMEIQAQEEITASETNA 700

BLAST of MC05g0336 vs. TAIR 10
Match: AT4G04850.1 (K+ efflux antiporter 3 )

HSP 1 Score: 307.0 bits (785), Expect = 6.8e-83
Identity = 200/525 (38.10%), Postives = 296/525 (56.38%), Query Frame = 0

Query: 612  DMLWLLLASVIFVPTFQKLPGGSPVLGYLAAGILIGPYGLSIIRHVHGTKAIAEFGVVFL 671
            D L  L+ +VI VP F+ L   SP+LG+  AG+++  +GL  IR++   K ++E+G++FL
Sbjct: 107  DTLTFLMVTVIIVPAFRILK-ASPILGFFFAGVVLNQFGL--IRNLTDVKVLSEWGILFL 166

Query: 672  LFNIGLELSVERLSSMKKYVFGLGSAQVLVT-------------AVVVGLVAHIVCGQPG 731
            LF +GLELS+ RL ++ K+ FG+G  QVL+              A+   ++  +   +P 
Sbjct: 167  LFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHSRPD 226

Query: 732  -------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLIL 791
                     A+VIG  L+LSS+A VLQ+L E+GE  +R G AT  +LL QD+AVV LL++
Sbjct: 227  LVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVI 286

Query: 792  IPLISPNSSKGGIGFQAIAEALGLAAVKAIVAISAIIAGGRLLLRPIYKQIAENQNAEIF 851
            +P++        IG ++I   L   + KA+  +  +  GG+  LR I++ +AE +++E F
Sbjct: 287  LPVLESQD----IGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAF 346

Query: 852  SANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMT 911
             A  LL + GTSL+T   G S  LGAFLAG LLAET F  Q+E+DI P+RGLLLGLFF+T
Sbjct: 347  VALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVT 406

Query: 912  VGMSIDPKLLLSNFPVISGSLGLLIGGKTILVALVGRLFGISIISALRVGLLLAPGGEFA 971
             G SID ++L   +P +   LG LI  KT+++  +G   G++I  ++RVG LL+ GGEFA
Sbjct: 407  TGTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFA 466

Query: 972  FVAFGEAVNQGIMSSHLSSLLFLVVGISMALTPWLAAGGQLIASRFEQHDVRSLLPVESE 1031
            FV F  A   G++ + L+ LL +VV +SMALTP+L   G+  A   ++         E  
Sbjct: 467  FVVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDV 526

Query: 1032 TDDLQDHIIICGFGRVGQIIAQLLSERLI--------PFVALDVRSDRVAVGRALDLPVY 1091
              D+ + I+I GFG++GQ++A  LS  L+        P++  D+    V   R L  P+ 
Sbjct: 527  NFDVSESIVIIGFGQMGQVLANFLSTPLVSDSDLVGWPYIGFDLNPAVVKESRKLGFPIL 586

Query: 1092 FGDAGSREVLHKVGAERACAAAITLDSPGANYRTVWALSKYFPNV 1109
            +GD     VL   G     A  I           V  L   FP V
Sbjct: 587  YGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGV 624