MC05g0332 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0332
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptiondigalactosyldiacylglycerol synthase 2, chloroplastic
LocationMC05: 2516493 .. 2520340 (+)
RNA-Seq ExpressionMC05g0332
SyntenyMC05g0332
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTATACTGGTTCTAAACTTGTAACACAACATCCAACTGTCCAAGCAACGCCAAGAAAAAAGAAATTGAACTTCACAACTCAGCAGGGAACGACGTCGTCGAGGCTTTGCTTCCATTTGCAGAAATGCATCCTCCAACGGCAATTGGTCGAATATTCCCCCTCTTTTTTTGTCTCTCTCCTCTATCTCGCTTTGCTGGTTGGCGCTGGATTTCCTCTCTCCTCTCCCTCCATCCATGGCTTTTCCCTGCTTCTGCTTCTGCTCATTCTCTCCCTCCCCCTAACATAACCTCGAGATTATTGGAGAACCCATATTATCATCATCTTCGTTGATTTGAACTCCCCGATTTCATGTTTTCTTCCGTTTTCTGCTCAATATTACTGTGAACTCTGCTGACGCCCAAGTTTCAATCATCGGTATATCTCTCTCACTCTCTCATATCATCATTCATTGATAGTATATGAAATCTGCATGATTCAATGTGCATTTGGCCGGTATAAAGGAATTCTGTGTAGGAATGAGAAAACGTAATTTATGTTTAGAACAATGCACGTGGCTACGAATACGAAGATTGAATTGAATTGGAAACGGAGTTATTTGTTTTCAACTCGAAGCAATGTTTGTATCTTAGGTTATTGAAGCTAGATTGTTAGGTTTTATCTCATAATTCTTTCCTATGAAATTTAAGAGGGGAAATGTGCATAATAGATATTTGATAAAGGTTGGTCGTGAATTTGATTATTTTTAACATTTATTTGGACAAATGTGTGGCTGAAACCTAAAATATTCTAATTGAGAATCAGGTTCCTCGATTATCAGGTGTCAATAAATTTCGGAAAGAGATTACAATGATGGTCATTTTTTGTGTTCATATCTGAGAAGGGGATGGATAGGAAGCAACATATTGCAATTTTTACCACTGCCAGTCTTCCATGGATGACTGGAACTGCAGTGAACCCTCTATTTCGTGCTGCCTATCTTTCAATTGGTGGGTATAGAAGGGTCACACTGGTGGTTCCTTGGCTATCTTTAAGGGATCAGGAACTTGTATATCCCAGCAGCACAACATTCATCCTACCCTCGCAGCAGGAGAAATACATTCGGAAATGGCTAGAAGAAAGAACAGGGTCAAAGTTCAATTTCAGCCTCCAATTTTATCCCGGGAAGGTATCTGTAGCATGATTAATTCTAACCCTCATCTTAGACACGACACATGATTTTTTTTTTTTGTGTGGCCAGGCCTCGGGACTAAGTATTTTTGGCTTTTTCATTTCTGGATTCATGACGTGTGCTGCTTTCTGTTCTTGTACTCATAAGCCAACTCAATATTTCACTTGATTCATTTGCAATTATCATTCTGTGTACTTGTTTGGCCTTAAGACTGGTTATGGTCCAACAGTCCTCTTGCCTGTGTTTCTTTTCAGTTTTTCTTTGCTCGCGTTGTTTTTCACATAAGCATGAAATTCTAATTCATTCCCTATCCTGCTCTTTTTACATTTTTTGGTGTTTAACGCACTAAGGTTTCACTTAATTATGCAGTTCTCCGTGGATAAAAGGAGCATTCTCCCCGTTGGTGATATTTCAGAAATTATCCCTGATGACGAATCAGATATCGCAATCCTCGAGGAGCCTGAGCATCTAACATGGTATCATCATGGAAGGAGATGGAAAAATAAATTTAAATTGGTCATAGGAATTATACATACCAACTATTTGGAATATGTTAAACGGGAGAAGAATGGGGCACTTGAAGCTTTTCTTCTTGAATATATAAATAGATGGGTCGTTCATATCTATTGCCACAAGGTATTTGTGCTATATGTTACTTCCAATGAAATAATCCAAATTGGGTTCATTGTGATCATTTTTTCATTCTTATTCCAGTTGACTTTTTCCTAGAAAATAAGTAAGTTGAATTAATTTGTTTTCACCATCGTAACTGGATGGCTTATTCATCAATTCTTGTATAAGCTTTCAGGTGATTAGGTTATCTGCTGCCACCCAGGATTATCCAAAGTCCATTATCTGCAATGTTCATGGAGTCAATCCCAAATTCCTCGAGATAGGTACGAAGAAGATGGAACAATTACAAAATGGGGAGCAAGTTTTTGGCAAAGGTGCCTATTATATAGGGAAGATGATATGGAGCAAGGGCTACAGGGAGCTGCTTAAACTTCTTAGCGATTACCAAAAGGATTTAGCTGGGCTTGAGATTGATTTATTTGGAAACGGAGAGGATGCTGACCAAGTCCAGAAAGCTACTGAAGACCTGGAATTATCTGTTAGAGTCCACGCCGGTCGTGATCACACCGATCCTATCTTCCATGAGTATGTATAACCACTTCTAGCTATTTTCATGTCCTCTACCTAGAGGATAAAGGAATTAATCCGTTCAATCATTTGTAACAATATTCAATGTCGCTGGTGATATTATTGGAAATTTTGCTTCATGATTTGCTGACAGAAAACGAAATAAAATAAAATAAAGAAAAGCTCAAGGGAACTGTTGACTACTGCAGTATCTTACGAACTAATATTGTGGAATGTATAAAGGAACTGATTGAATCATTTATTACCTTTGATCTTTTATGGTTGATTCTTGATTTTAAAACTTTTTTTTTTAGTTTAAAACTTTAAATTGATGATAATTGCTCCAACCATTTGATTGCCTTACCCTGACTGAATATAGAGTAAAAACGACTGAACTAAAGTGGACGGGAAAAAAGCAGGATGGGGTAGGATTCTTCCTTCAAATAAATCTGACCTGATTGCTCATAAGCACTGGTTTGTGATTTGGTAGGTTTGCCTCATTACTGCTCATCTAGAAGTGATAACTTTTTTCAGCTTCCTTAATCATTGATTTTTAACAGTTATGGCTAGTGATTTACTGGAACTAACATTGTGGGGGGCATTAGAACTGTGAATTGTCTCGAAAGAAGGTCGAGGCTCAGTTTTTTTTCTCTGTTGGTCTAAATAGTGTGTCCATTGGATGGTTTTAATTTTTCTTTCTTTCTTTTTCAGTTACAAAGTCTTTCTGAATCCAAGCACCACAGACGTTGTTTGCACTACCACAGCTGAAGCATTGGCTATGGGCAAATTTGTTGTATGTGCTGATCATCCCTCAAATGAGTTCTTCAGGCAATTTCCAAACTGCATGCTCTATGACAACAGTGATGGTTTTGTTAGAGCAGTTTTCAAAGCACTGACTGAAGAGCCCGAGCAGCTGACGGATGCACAGAGGCACGAGCTCACGTGGGAGGCTGCAACAGAACGTTTCTTAAAGGCTTCTGAACTCGACCAGCCACTTATGAGGAAGCCTCAGAAAAATCGTTCGAAGAAGTTTCTATCTCTATCACTGCAACTTAGTAGAAAATTTGAAGATGCATCAGCATATATACATCATGCGACTCTTGGGTTTGAGGGGCCTCGAAGAATGTTCGGTGCCGTCCCCGGGAGCCTGTATCCAGATGAACAGCAGTGCAAAGAGCTTGGATTAGCTCCTCCTAGCAAAAGAGGTTCGAAAAAGTAATATTTTGACCATATCATTTATGATCCCATTCATAATCTTTCCGAGGATGTTAGATTTTCTATTCTAGGTGGATTGTAAATTTGAAAGATTGTCCACATCTGTCCTCCTTATTCTTACAGTCTATTGTTGTCTGAAGCTGAGACTGGTAGTTATAGAGTTTAACAGCAGGAATGATTAACCAGGATTAGAAGAGAAAATAAGATTTAGCCAAACATGAAACATGTTATCAAATACAGTAATATTTTAACAAGTTCTTGTACTTTATTGATTTATTTGTTCGTTGCTTTTTTTGGAAAAATATTATTTCTCTATCATACCA

mRNA sequence

ATTTATACTGGTTCTAAACTTGTAACACAACATCCAACTGTCCAAGCAACGCCAAGAAAAAAGAAATTGAACTTCACAACTCAGCAGGGAACGACGTCGTCGAGGCTTTGCTTCCATTTGCAGAAATGCATCCTCCAACGGCAATTGGTCGAATATTCCCCCTCTTTTTTTGTCTCTCTCCTCTATCTCGCTTTGCTGGTTGGCGCTGGATTTCCTCTCTCCTCTCCCTCCATCCATGGCTTTTCCCTGCTTCTGCTTCTGCTCATTCTCTCCCTCCCCCTAACATAACCTCGAGATTATTGGAGAACCCATATTATCATCATCTTCGTTGATTTGAACTCCCCGATTTCATGTTTTCTTCCGTTTTCTGCTCAATATTACTGTGAACTCTGCTGACGCCCAAGTTTCAATCATCGGTTCCTCGATTATCAGGTGTCAATAAATTTCGGAAAGAGATTACAATGATGGTCATTTTTTGTGTTCATATCTGAGAAGGGGATGGATAGGAAGCAACATATTGCAATTTTTACCACTGCCAGTCTTCCATGGATGACTGGAACTGCAGTGAACCCTCTATTTCGTGCTGCCTATCTTTCAATTGGTGGGTATAGAAGGGTCACACTGGTGGTTCCTTGGCTATCTTTAAGGGATCAGGAACTTGTATATCCCAGCAGCACAACATTCATCCTACCCTCGCAGCAGGAGAAATACATTCGGAAATGGCTAGAAGAAAGAACAGGGTCAAAGTTCAATTTCAGCCTCCAATTTTATCCCGGGAAGTTCTCCGTGGATAAAAGGAGCATTCTCCCCGTTGGTGATATTTCAGAAATTATCCCTGATGACGAATCAGATATCGCAATCCTCGAGGAGCCTGAGCATCTAACATGGTATCATCATGGAAGGAGATGGAAAAATAAATTTAAATTGGTCATAGGAATTATACATACCAACTATTTGGAATATGTTAAACGGGAGAAGAATGGGGCACTTGAAGCTTTTCTTCTTGAATATATAAATAGATGGGTCGTTCATATCTATTGCCACAAGGTGATTAGGTTATCTGCTGCCACCCAGGATTATCCAAAGTCCATTATCTGCAATGTTCATGGAGTCAATCCCAAATTCCTCGAGATAGGTACGAAGAAGATGGAACAATTACAAAATGGGGAGCAAGTTTTTGGCAAAGGTGCCTATTATATAGGGAAGATGATATGGAGCAAGGGCTACAGGGAGCTGCTTAAACTTCTTAGCGATTACCAAAAGGATTTAGCTGGGCTTGAGATTGATTTATTTGGAAACGGAGAGGATGCTGACCAAGTCCAGAAAGCTACTGAAGACCTGGAATTATCTGTTAGAGTCCACGCCGGTCGTGATCACACCGATCCTATCTTCCATGATTACAAAGTCTTTCTGAATCCAAGCACCACAGACGTTGTTTGCACTACCACAGCTGAAGCATTGGCTATGGGCAAATTTGTTGTATGTGCTGATCATCCCTCAAATGAGTTCTTCAGGCAATTTCCAAACTGCATGCTCTATGACAACAGTGATGGTTTTGTTAGAGCAGTTTTCAAAGCACTGACTGAAGAGCCCGAGCAGCTGACGGATGCACAGAGGCACGAGCTCACGTGGGAGGCTGCAACAGAACGTTTCTTAAAGGCTTCTGAACTCGACCAGCCACTTATGAGGAAGCCTCAGAAAAATCGTTCGAAGAAGTTTCTATCTCTATCACTGCAACTTAGTAGAAAATTTGAAGATGCATCAGCATATATACATCATGCGACTCTTGGGTTTGAGGGGCCTCGAAGAATGTTCGGTGCCGTCCCCGGGAGCCTGTATCCAGATGAACAGCAGTGCAAAGAGCTTGGATTAGCTCCTCCTAGCAAAAGAGGTTCGAAAAAGTAATATTTTGACCATATCATTTATGATCCCATTCATAATCTTTCCGAGGATGTTAGATTTTCTATTCTAGGTGGATTGTAAATTTGAAAGATTGTCCACATCTGTCCTCCTTATTCTTACAGTCTATTGTTGTCTGAAGCTGAGACTGGTAGTTATAGAGTTTAACAGCAGGAATGATTAACCAGGATTAGAAGAGAAAATAAGATTTAGCCAAACATGAAACATGTTATCAAATACAGTAATATTTTAACAAGTTCTTGTACTTTATTGATTTATTTGTTCGTTGCTTTTTTTGGAAAAATATTATTTCTCTATCATACCA

Coding sequence (CDS)

ATGGATAGGAAGCAACATATTGCAATTTTTACCACTGCCAGTCTTCCATGGATGACTGGAACTGCAGTGAACCCTCTATTTCGTGCTGCCTATCTTTCAATTGGTGGGTATAGAAGGGTCACACTGGTGGTTCCTTGGCTATCTTTAAGGGATCAGGAACTTGTATATCCCAGCAGCACAACATTCATCCTACCCTCGCAGCAGGAGAAATACATTCGGAAATGGCTAGAAGAAAGAACAGGGTCAAAGTTCAATTTCAGCCTCCAATTTTATCCCGGGAAGTTCTCCGTGGATAAAAGGAGCATTCTCCCCGTTGGTGATATTTCAGAAATTATCCCTGATGACGAATCAGATATCGCAATCCTCGAGGAGCCTGAGCATCTAACATGGTATCATCATGGAAGGAGATGGAAAAATAAATTTAAATTGGTCATAGGAATTATACATACCAACTATTTGGAATATGTTAAACGGGAGAAGAATGGGGCACTTGAAGCTTTTCTTCTTGAATATATAAATAGATGGGTCGTTCATATCTATTGCCACAAGGTGATTAGGTTATCTGCTGCCACCCAGGATTATCCAAAGTCCATTATCTGCAATGTTCATGGAGTCAATCCCAAATTCCTCGAGATAGGTACGAAGAAGATGGAACAATTACAAAATGGGGAGCAAGTTTTTGGCAAAGGTGCCTATTATATAGGGAAGATGATATGGAGCAAGGGCTACAGGGAGCTGCTTAAACTTCTTAGCGATTACCAAAAGGATTTAGCTGGGCTTGAGATTGATTTATTTGGAAACGGAGAGGATGCTGACCAAGTCCAGAAAGCTACTGAAGACCTGGAATTATCTGTTAGAGTCCACGCCGGTCGTGATCACACCGATCCTATCTTCCATGATTACAAAGTCTTTCTGAATCCAAGCACCACAGACGTTGTTTGCACTACCACAGCTGAAGCATTGGCTATGGGCAAATTTGTTGTATGTGCTGATCATCCCTCAAATGAGTTCTTCAGGCAATTTCCAAACTGCATGCTCTATGACAACAGTGATGGTTTTGTTAGAGCAGTTTTCAAAGCACTGACTGAAGAGCCCGAGCAGCTGACGGATGCACAGAGGCACGAGCTCACGTGGGAGGCTGCAACAGAACGTTTCTTAAAGGCTTCTGAACTCGACCAGCCACTTATGAGGAAGCCTCAGAAAAATCGTTCGAAGAAGTTTCTATCTCTATCACTGCAACTTAGTAGAAAATTTGAAGATGCATCAGCATATATACATCATGCGACTCTTGGGTTTGAGGGGCCTCGAAGAATGTTCGGTGCCGTCCCCGGGAGCCTGTATCCAGATGAACAGCAGTGCAAAGAGCTTGGATTAGCTCCTCCTAGCAAAAGAGGTTCGAAAAAGTAA

Protein sequence

MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSSTTFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIAILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIYCHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWSKGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHDYKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKALTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFEDASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPSKRGSKK
Homology
BLAST of MC05g0332 vs. ExPASy Swiss-Prot
Match: Q6DW75 (Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Glycine max OX=3847 GN=DGD2 PE=2 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 4.6e-202
Identity = 335/464 (72.20%), Postives = 389/464 (83.84%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MD+K+HIAIFTTASLPW+TGTAVNPLFRAAYL+  G R VTLV+PWLSL+DQ LVYP++ 
Sbjct: 1   MDKKEHIAIFTTASLPWLTGTAVNPLFRAAYLAKSGERDVTLVIPWLSLKDQRLVYPNNI 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TF  PS+ EKYI +WLEER G    FS+QFYPGKFS DKRSIL VGDISEIIPD  +DIA
Sbjct: 61  TFASPSEHEKYICQWLEERVGFTSGFSIQFYPGKFSRDKRSILAVGDISEIIPDKVADIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHG+RWK KF+LVIGIIHTNYLEYVKREKNG ++AFLL+Y+N WVV IY
Sbjct: 121 VLEEPEHLTWYHHGKRWKTKFRLVIGIIHTNYLEYVKREKNGVMQAFLLKYLNNWVVSIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDY  SIICNVHGVNPKFLEIG KK EQ Q GE  F KGAY+IGKMIWS
Sbjct: 181 CHKVIRLSAATQDYTGSIICNVHGVNPKFLEIGKKKREQQQKGEHAFTKGAYFIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGY+ELL+LL D++K+L+ LE+DLFG+GED+D+VQKA E LEL+VRVH  RDH D +FHD
Sbjct: 241 KGYKELLQLLKDHEKELSALEVDLFGSGEDSDEVQKAAEKLELAVRVHPARDHADALFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YK+FLNPSTTDVVCTTTAEALAMGK VVCA+HPSN+FF+QFPNC  YD+ DGFV+   KA
Sbjct: 301 YKLFLNPSTTDVVCTTTAEALAMGKIVVCANHPSNDFFKQFPNCWTYDDDDGFVKLTLKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           L E+P Q TDAQRH+L+WEAAT+RFLKA++LD+PL RK  +  S  FL+ SL L  K ++
Sbjct: 361 LAEQPAQPTDAQRHDLSWEAATKRFLKAADLDKPLERKLSRTTS-NFLAASLNLQEKVDE 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPSKR 465
           ASAY+HH   GFE  RR+FGA+P SL PDE+  KELGL   S +
Sbjct: 421 ASAYVHHVASGFEVSRRIFGAIPDSLQPDEELRKELGLTDASTK 463

BLAST of MC05g0332 vs. ExPASy Swiss-Prot
Match: Q6DW73 (Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Lotus japonicus OX=34305 GN=DGD2 PE=2 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 1.4e-201
Identity = 332/464 (71.55%), Postives = 392/464 (84.48%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           M +KQHIAIFTTASLPW+TGTAVNPLFRAAYLS  G R VTLV+PWLSL+DQ LVYP++ 
Sbjct: 1   MGKKQHIAIFTTASLPWLTGTAVNPLFRAAYLSKDGERDVTLVIPWLSLKDQALVYPNNI 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TF  PS+ EKYIR+WLEER G    FS++FYPGKFS DKRSIL VGDISE+IPD E+DIA
Sbjct: 61  TFASPSEHEKYIRQWLEERVGFTSGFSIKFYPGKFSRDKRSILAVGDISEVIPDKEADIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           +LEEPEHLTW+HHG+RWK KF+LVIGIIHTNYLEYVKREKNG ++AFLL+Y+N WVV IY
Sbjct: 121 VLEEPEHLTWFHHGKRWKTKFRLVIGIIHTNYLEYVKREKNGQMQAFLLKYLNNWVVGIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDY  SI+CNVHGVNPKFLEIG KK EQ QNG+Q F KGAY+IGKM+WS
Sbjct: 181 CHKVIRLSAATQDYSGSIVCNVHGVNPKFLEIGKKKREQQQNGDQAFTKGAYFIGKMVWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGY+ELL L  ++QK+L+ LE+DLFG+GED+D+VQKA + LE++VRVH  RDH D +FHD
Sbjct: 241 KGYKELLHLFKNHQKELSALEVDLFGSGEDSDEVQKAAKKLEMAVRVHPARDHADALFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YK+FLNPSTTDVVCTTTAEALAMGK VVCA+H SNEFF+QFPNC  +D S GFV+ + KA
Sbjct: 301 YKLFLNPSTTDVVCTTTAEALAMGKIVVCANHCSNEFFKQFPNCWTFDESKGFVQLILKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           L EEP QLTDAQRH+L+WEAATERFLKA+ELD+P  +K  ++ S  ++S SL L +  +D
Sbjct: 361 LAEEPAQLTDAQRHDLSWEAATERFLKAAELDKPFEKKLSRSTS-IYMSTSLNLQQTVDD 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPSKR 465
           ASAY+HH   GFE  RRMFGA+PGSL PDE+  KELGL+   ++
Sbjct: 421 ASAYVHHVASGFEISRRMFGAIPGSLKPDEELSKELGLSDSGRK 463

BLAST of MC05g0332 vs. ExPASy Swiss-Prot
Match: Q8W1S1 (Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGD2 PE=1 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 4.5e-189
Identity = 306/459 (66.67%), Postives = 382/459 (83.22%), Query Frame = 0

Query: 3   RKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSSTTF 62
           ++QHIAIFTTAS+PW+TGTAVNPLFRAAYL+  G RRVTLV+PWL+L+ Q+LVYP+S TF
Sbjct: 5   QEQHIAIFTTASIPWLTGTAVNPLFRAAYLANDGERRVTLVIPWLTLKHQKLVYPNSITF 64

Query: 63  ILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIAIL 122
             PS+QE Y+R+WLEER   +  F ++FYPGKF++DKRSILPVGDIS+ IPD+E+DIA+L
Sbjct: 65  SSPSEQEAYVRQWLEERVSFRLAFEIRFYPGKFAIDKRSILPVGDISDAIPDEEADIAVL 124

Query: 123 EEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIYCH 182
           EEPEHLTW+HHG++WK KF  VIGI+HTNYLEYVKREK G ++AF L+Y+N WVV IYCH
Sbjct: 125 EEPEHLTWFHHGQKWKTKFNYVIGIVHTNYLEYVKREKQGRVKAFFLKYLNSWVVGIYCH 184

Query: 183 KVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWSKG 242
           KVIRLSAATQ+YPKSI+CNVHGVNPKFLEIG +K+EQ +  EQ F KGAYYIGKM+WSKG
Sbjct: 185 KVIRLSAATQEYPKSIVCNVHGVNPKFLEIGLRKLEQQKLQEQPFTKGAYYIGKMVWSKG 244

Query: 243 YRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHDYK 302
           Y+ELLKLL  +QK+LA LE+DL+G+GED++++++A   L+L+V V+ GRDH D +FH+YK
Sbjct: 245 YKELLKLLEKHQKELAELEVDLYGDGEDSEEIKEAARKLDLTVNVYPGRDHADSLFHNYK 304

Query: 303 VFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKALT 362
           VFLNPSTTDVVCTTTAEALAMGK VVCA+H SN+FF+QFPNC  YD+  GFVRA  KAL 
Sbjct: 305 VFLNPSTTDVVCTTTAEALAMGKIVVCANHISNKFFKQFPNCRTYDDGQGFVRATLKALG 364

Query: 363 EEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKK--FLSLSLQLSRKFED 422
           E+P QLT+ QRHEL+WEAAT+RF+K S+L++  + +   N SK+  F S S+ + +  ED
Sbjct: 365 EQPSQLTEQQRHELSWEAATQRFIKVSDLNR--LSRADSNLSKRSVFASSSISVGKNLED 424

Query: 423 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLA 460
            SAYIH    GFE  R  FGA+PGSL PDE+ C++LGL+
Sbjct: 425 MSAYIHFLASGFEASRTAFGAIPGSLQPDEELCRDLGLS 461

BLAST of MC05g0332 vs. ExPASy Swiss-Prot
Match: Q6DW76 (Digalactosyldiacylglycerol synthase 1, chloroplastic OS=Glycine max OX=3847 GN=DGD1 PE=2 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 1.9e-163
Identity = 274/468 (58.55%), Postives = 349/468 (74.57%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           +D ++H+AI TTASLPWMTGTAVNPLFRAAYLS    ++VTL+VPWL   DQELVYPS+ 
Sbjct: 307 LDNERHVAIVTTASLPWMTGTAVNPLFRAAYLSQSAKQKVTLLVPWLCKSDQELVYPSNL 366

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TF  P +QE YIR WLEER G K +F + FYPGKFS  +RSI+P GD S+ IP  ++DIA
Sbjct: 367 TFTSPEEQEAYIRSWLEERIGFKADFKISFYPGKFSEARRSIIPAGDTSQFIPSRDADIA 426

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           ILEEPEHL WYHHG+RW +KF  V+GI+HTNYLEY+KREKNGAL+AFL+++IN WV   Y
Sbjct: 427 ILEEPEHLNWYHHGKRWTDKFNHVVGIVHTNYLEYIKREKNGALQAFLVKHINNWVTRAY 486

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKV+RLSAATQD PKS+ICNVHGVNPKFL+IG K   + + G++ F KGAY++GKM+W+
Sbjct: 487 CHKVLRLSAATQDLPKSVICNVHGVNPKFLKIGEKIAAERELGQKAFTKGAYFLGKMVWA 546

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGY+EL+ LL+ ++ DL G ++D+FGNGEDA++VQ A   L+L++    GRDH D   H 
Sbjct: 547 KGYKELIDLLAKHKADLDGFKLDVFGNGEDANEVQSAARRLDLNLNFQKGRDHADDSLHR 606

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVF+NPS +DV+CT TAEALAMGKFVVCADHPSNEFFR FPNC+ Y  S+ FV  V +A
Sbjct: 607 YKVFINPSISDVLCTATAEALAMGKFVVCADHPSNEFFRSFPNCLTYRTSEDFVTKVKEA 666

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSK----KFLSLSL---Q 420
           L  EP  LT  QR++L+WEAAT+RF++ SELD  L ++    +S+    K ++ S     
Sbjct: 667 LENEPYPLTPEQRYQLSWEAATQRFMEYSELDGILNKENNGEKSRVDKGKLIAKSASMPN 726

Query: 421 LSRKFEDASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPP 462
           L+   +   A+ H+   G E  R   GA+PG+   D+Q CK+L L PP
Sbjct: 727 LTELVDGGLAFAHYCLTGNEFLRLCTGAIPGTRDYDKQHCKDLHLLPP 774

BLAST of MC05g0332 vs. ExPASy Swiss-Prot
Match: Q6DW74 (Digalactosyldiacylglycerol synthase 1, chloroplastic OS=Lotus japonicus OX=34305 GN=DGD1 PE=2 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 3.4e-160
Identity = 270/468 (57.69%), Postives = 345/468 (73.72%), Query Frame = 0

Query: 2   DRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSSTT 61
           D ++H+AI TTASLPWMTGTAVNPLFRAAYLS    ++VTL+VPWL   DQELVYPS+ T
Sbjct: 311 DNERHVAIVTTASLPWMTGTAVNPLFRAAYLSQSEKQKVTLLVPWLCKSDQELVYPSNLT 370

Query: 62  FILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIAI 121
           F  P +QE YIR WLEER G K +F + FYPGKFS  +RSI+P GD ++ IP  ++DIAI
Sbjct: 371 FTSPEEQEGYIRNWLEERIGFKADFKISFYPGKFSQARRSIIPAGDTAQFIPSKDADIAI 430

Query: 122 LEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIYC 181
           LEEPEHL WYHHG RW +KF  V+GI+HTNYLEY+KREKNGAL+AFL+++IN WV   YC
Sbjct: 431 LEEPEHLNWYHHGTRWTDKFNHVVGIVHTNYLEYIKREKNGALQAFLVKHINNWVARAYC 490

Query: 182 HKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWSK 241
            KV+RLSAATQD PKS++CNVHGVNPKFL+IG     + + G++ F KGAY++GKM+W+K
Sbjct: 491 DKVLRLSAATQDLPKSVVCNVHGVNPKFLKIGESIAAERELGQKGFTKGAYFLGKMVWAK 550

Query: 242 GYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHDY 301
           GY+EL+ LL+ ++ DL G+++D+FGNGEDA++VQ A    +L++    GRDH D   H Y
Sbjct: 551 GYKELIDLLAKHKADLDGVKLDVFGNGEDANEVQSAARRFDLNLNFQKGRDHADDSLHRY 610

Query: 302 KVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKAL 361
           KVF+NPS +DV+CT TAEALAMGKFVVCADHPSNEFFR FPNC+ Y   + F   V +AL
Sbjct: 611 KVFINPSISDVLCTATAEALAMGKFVVCADHPSNEFFRSFPNCLTYKTPEDFAVKVKEAL 670

Query: 362 TEEPEQLTDAQRHELTWEAATERFLKASELDQPLMR-----KPQKNRSKKFLSLSL---Q 421
             EP  LT  QR++L+WEAAT+RF++ SELD+ L +     KP KN ++K ++ S     
Sbjct: 671 ANEPYPLTPEQRYQLSWEAATQRFMEYSELDKVLNKEKDGAKPSKN-NRKIMAKSASMPN 730

Query: 422 LSRKFEDASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPP 462
           L+   +   A+ H+   G E  R   GA PG+   D+Q CK+L L PP
Sbjct: 731 LTELVDGGLAFAHYCLTGNEFLRLCTGATPGTRDYDKQHCKDLNLLPP 777

BLAST of MC05g0332 vs. NCBI nr
Match: XP_022147482.1 (digalactosyldiacylglycerol synthase 2, chloroplastic [Momordica charantia] >XP_022147483.1 digalactosyldiacylglycerol synthase 2, chloroplastic [Momordica charantia])

HSP 1 Score: 955 bits (2468), Expect = 0.0
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST
Sbjct: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA
Sbjct: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD
Sbjct: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED
Sbjct: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPSKRGSKK 468
           ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPSKRGSKK
Sbjct: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPSKRGSKK 468

BLAST of MC05g0332 vs. NCBI nr
Match: XP_008438079.1 (PREDICTED: digalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis melo] >XP_008438080.1 PREDICTED: digalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis melo] >KAA0048985.1 digalactosyldiacylglycerol synthase 2 [Cucumis melo var. makuwa] >TYK17579.1 digalactosyldiacylglycerol synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 423/469 (90.19%), Postives = 453/469 (96.59%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLS+ G R++TLVVPWLSLRDQELVYPS+T
Sbjct: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSMDGDRKITLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TFILPSQQEKYIR+WLEERTGSKFNF+LQFYPGKF+VDKRSILPVGDISEII +DESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLQFYPGKFAVDKRSILPVGDISEIIHEDESDIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           ILEEPEHLTWYHHG+RWKNKF LVIGIIHTNYLEYVKREKNGA+EAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGKRWKNKFTLVIGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIG KK+EQ +NG QVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKIEQQKNGGQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGYRELLKLL+DYQKDLA LE+DLFGNGEDAD+VQKA++DLELSV+VH GRDHTDPIFHD
Sbjct: 241 KGYRELLKLLTDYQKDLAELEVDLFGNGEDADEVQKASQDLELSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCA+HPSNEFF+QFPNC+LYDNSDGFV+A+FKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCILYDNSDGFVKAIFKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           L+EEPEQLTDAQRHEL+WEAATERFLKA+ELDQPL RKPQK RSKKFLSLSLQL RKF+D
Sbjct: 361 LSEEPEQLTDAQRHELSWEAATERFLKAAELDQPLARKPQKTRSKKFLSLSLQLGRKFDD 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPS-KRGSKK 468
           ASAYIHH TLGFEG RR+FGAVPGSL+PDEQQCKELGLA PS +RGSKK
Sbjct: 421 ASAYIHHMTLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSRRGSKK 469

BLAST of MC05g0332 vs. NCBI nr
Match: XP_038878183.1 (digalactosyldiacylglycerol synthase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 872 bits (2254), Expect = 0.0
Identity = 424/469 (90.41%), Postives = 448/469 (95.52%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MDR+QHIAIFTTASLPWMTGTAVNPLFRAAYLSIGG R++TLVVPWLSLRDQELVYPS+T
Sbjct: 1   MDREQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGDRKITLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TFILPSQQEKYIR+WLEERTGSKFNF+LQFYPGKF+VDKRSILPVGDISEIIPDDESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLQFYPGKFAVDKRSILPVGDISEIIPDDESDIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           ILEEPEHLTWYHHG+RWKNKFKLVIGIIHTNYLEYVKREKNGA+EAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGKRWKNKFKLVIGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSI CNVHGVNPKFLEIG KKMEQ Q G QVFG+GAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSITCNVHGVNPKFLEIGKKKMEQQQIGGQVFGRGAYYIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGYRELLKLLS+YQ +L G+E+DLFGNGEDAD+VQKAT+DLELSVRVH GRDHTDPIFHD
Sbjct: 241 KGYRELLKLLSNYQTELDGIEVDLFGNGEDADEVQKATQDLELSVRVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNC+LYDN DGFV+AVFKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCILYDNGDGFVKAVFKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           L EEPEQLTDAQRH L+WEAATERFL A+ELDQPL RKPQ+ RSKKFLSLS QL RKF+D
Sbjct: 361 LNEEPEQLTDAQRHVLSWEAATERFLTAAELDQPLARKPQRTRSKKFLSLSSQLGRKFDD 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLA-PPSKRGSKK 468
           ASAYIHH TLGFEGPRR+FGAVPGSL PDEQQCKELGLA P SKRGS+K
Sbjct: 421 ASAYIHHMTLGFEGPRRVFGAVPGSLDPDEQQCKELGLARPSSKRGSRK 469

BLAST of MC05g0332 vs. NCBI nr
Match: XP_004133875.1 (digalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis sativus] >KGN56539.1 hypothetical protein Csa_010433 [Cucumis sativus])

HSP 1 Score: 866 bits (2238), Expect = 0.0
Identity = 419/469 (89.34%), Postives = 448/469 (95.52%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MDRKQHIAIFTTA LPWMTGTAVNPLFRAAYLS+ G R+VTLVVPWLSLRDQELVYPS+T
Sbjct: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TFILPSQQEKYIR+WLEERTGSKFNF+L FYPGKF+VDKRSILPVGDISEIIPD ESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHG+RWKNKF LV+GIIHTNYLEYVKREKNGA+EAFLLEYINRWVVHIY
Sbjct: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIG KKMEQ QNG QVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGYREL+KLLSDYQKD A LE+DLFGNGED+D+VQKAT+DLE+SV+VH GRDHTDPIFHD
Sbjct: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCA+HPSNEFF+QFPNC+LYDN+DGFV+AVFKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           L EEPEQLTDAQRHEL+WEAATERFLKA+ELDQ L RKPQK RSKKFLSLSLQL RKF+D
Sbjct: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPS-KRGSKK 468
           A+AYIHH +LGFEG RR+FGAVPGSL+PDEQQCKELGLA PS KRGSKK
Sbjct: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 469

BLAST of MC05g0332 vs. NCBI nr
Match: XP_022975105.1 (digalactosyldiacylglycerol synthase 2, chloroplastic [Cucurbita maxima] >XP_022975106.1 digalactosyldiacylglycerol synthase 2, chloroplastic [Cucurbita maxima] >XP_022975107.1 digalactosyldiacylglycerol synthase 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 848 bits (2192), Expect = 5.36e-309
Identity = 410/469 (87.42%), Postives = 438/469 (93.39%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MD KQHIAIFTTASLPWMTGTAVNPLFRAAYLSIG  R+VTLVVPWLS RDQELVYPS+T
Sbjct: 1   MDTKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGEDRKVTLVVPWLSKRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TF+LPSQQEKYIR+WLEER GSKFNF+LQFYPGKF+VDKRSILPVGDISEIIPDDESDIA
Sbjct: 61  TFVLPSQQEKYIREWLEERIGSKFNFNLQFYPGKFAVDKRSILPVGDISEIIPDDESDIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           ILEEPEHLTWYHHGRRWK KFK V+GIIHTNYLEYVKREKNGA+EAFLLE+INRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGRRWKKKFKWVVGIIHTNYLEYVKREKNGAVEAFLLEHINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKS+ICNVHGVNPKFLEIG KKMEQ Q+ EQVFGKG YYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSVICNVHGVNPKFLEIGKKKMEQRQDEEQVFGKGVYYIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGYRELLKLLSD+QKDLAG+E+DLFGNGED+D+VQKA  DLELSV+VH GRDHTDPIFHD
Sbjct: 241 KGYRELLKLLSDHQKDLAGIEVDLFGNGEDSDEVQKAARDLELSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVF+NPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNC L D SD FVR +FKA
Sbjct: 301 YKVFVNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCFLCDKSDAFVRGIFKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           LTEEPE LT+ Q HEL+WEAATERFL A+ELDQPL +KPQ+ RSKKFLS+SLQL RKF+D
Sbjct: 361 LTEEPEPLTNVQMHELSWEAATERFLTAAELDQPLAKKPQRTRSKKFLSMSLQLGRKFDD 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPS-KRGSKK 468
           ASAYIHH TLGFEG RR FGAVPGSL+PDEQQCKELG+A PS KRGSKK
Sbjct: 421 ASAYIHHVTLGFEGSRRAFGAVPGSLHPDEQQCKELGIASPSGKRGSKK 469

BLAST of MC05g0332 vs. ExPASy TrEMBL
Match: A0A6J1D1F6 (digalactosyldiacylglycerol synthase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016394 PE=3 SV=1)

HSP 1 Score: 955 bits (2468), Expect = 0.0
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST
Sbjct: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA
Sbjct: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD
Sbjct: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED
Sbjct: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPSKRGSKK 468
           ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPSKRGSKK
Sbjct: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPSKRGSKK 468

BLAST of MC05g0332 vs. ExPASy TrEMBL
Match: A0A1S3AV60 (digalactosyldiacylglycerol synthase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483294 PE=3 SV=1)

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 423/469 (90.19%), Postives = 453/469 (96.59%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLS+ G R++TLVVPWLSLRDQELVYPS+T
Sbjct: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSMDGDRKITLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TFILPSQQEKYIR+WLEERTGSKFNF+LQFYPGKF+VDKRSILPVGDISEII +DESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLQFYPGKFAVDKRSILPVGDISEIIHEDESDIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           ILEEPEHLTWYHHG+RWKNKF LVIGIIHTNYLEYVKREKNGA+EAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGKRWKNKFTLVIGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIG KK+EQ +NG QVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKIEQQKNGGQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGYRELLKLL+DYQKDLA LE+DLFGNGEDAD+VQKA++DLELSV+VH GRDHTDPIFHD
Sbjct: 241 KGYRELLKLLTDYQKDLAELEVDLFGNGEDADEVQKASQDLELSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCA+HPSNEFF+QFPNC+LYDNSDGFV+A+FKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCILYDNSDGFVKAIFKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           L+EEPEQLTDAQRHEL+WEAATERFLKA+ELDQPL RKPQK RSKKFLSLSLQL RKF+D
Sbjct: 361 LSEEPEQLTDAQRHELSWEAATERFLKAAELDQPLARKPQKTRSKKFLSLSLQLGRKFDD 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPS-KRGSKK 468
           ASAYIHH TLGFEG RR+FGAVPGSL+PDEQQCKELGLA PS +RGSKK
Sbjct: 421 ASAYIHHMTLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSRRGSKK 469

BLAST of MC05g0332 vs. ExPASy TrEMBL
Match: A0A5D3D3K6 (Digalactosyldiacylglycerol synthase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004490 PE=3 SV=1)

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 423/469 (90.19%), Postives = 453/469 (96.59%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLS+ G R++TLVVPWLSLRDQELVYPS+T
Sbjct: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSMDGDRKITLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TFILPSQQEKYIR+WLEERTGSKFNF+LQFYPGKF+VDKRSILPVGDISEII +DESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLQFYPGKFAVDKRSILPVGDISEIIHEDESDIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           ILEEPEHLTWYHHG+RWKNKF LVIGIIHTNYLEYVKREKNGA+EAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGKRWKNKFTLVIGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIG KK+EQ +NG QVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKIEQQKNGGQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGYRELLKLL+DYQKDLA LE+DLFGNGEDAD+VQKA++DLELSV+VH GRDHTDPIFHD
Sbjct: 241 KGYRELLKLLTDYQKDLAELEVDLFGNGEDADEVQKASQDLELSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCA+HPSNEFF+QFPNC+LYDNSDGFV+A+FKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCILYDNSDGFVKAIFKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           L+EEPEQLTDAQRHEL+WEAATERFLKA+ELDQPL RKPQK RSKKFLSLSLQL RKF+D
Sbjct: 361 LSEEPEQLTDAQRHELSWEAATERFLKAAELDQPLARKPQKTRSKKFLSLSLQLGRKFDD 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPS-KRGSKK 468
           ASAYIHH TLGFEG RR+FGAVPGSL+PDEQQCKELGLA PS +RGSKK
Sbjct: 421 ASAYIHHMTLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSRRGSKK 469

BLAST of MC05g0332 vs. ExPASy TrEMBL
Match: A0A0A0L6B2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122630 PE=3 SV=1)

HSP 1 Score: 866 bits (2238), Expect = 0.0
Identity = 419/469 (89.34%), Postives = 448/469 (95.52%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MDRKQHIAIFTTA LPWMTGTAVNPLFRAAYLS+ G R+VTLVVPWLSLRDQELVYPS+T
Sbjct: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TFILPSQQEKYIR+WLEERTGSKFNF+L FYPGKF+VDKRSILPVGDISEIIPD ESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHG+RWKNKF LV+GIIHTNYLEYVKREKNGA+EAFLLEYINRWVVHIY
Sbjct: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIG KKMEQ QNG QVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGYREL+KLLSDYQKD A LE+DLFGNGED+D+VQKAT+DLE+SV+VH GRDHTDPIFHD
Sbjct: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCA+HPSNEFF+QFPNC+LYDN+DGFV+AVFKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           L EEPEQLTDAQRHEL+WEAATERFLKA+ELDQ L RKPQK RSKKFLSLSLQL RKF+D
Sbjct: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPS-KRGSKK 468
           A+AYIHH +LGFEG RR+FGAVPGSL+PDEQQCKELGLA PS KRGSKK
Sbjct: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 469

BLAST of MC05g0332 vs. ExPASy TrEMBL
Match: A0A6J1IFT4 (digalactosyldiacylglycerol synthase 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111474080 PE=3 SV=1)

HSP 1 Score: 848 bits (2192), Expect = 2.59e-309
Identity = 410/469 (87.42%), Postives = 438/469 (93.39%), Query Frame = 0

Query: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60
           MD KQHIAIFTTASLPWMTGTAVNPLFRAAYLSIG  R+VTLVVPWLS RDQELVYPS+T
Sbjct: 1   MDTKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGEDRKVTLVVPWLSKRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120
           TF+LPSQQEKYIR+WLEER GSKFNF+LQFYPGKF+VDKRSILPVGDISEIIPDDESDIA
Sbjct: 61  TFVLPSQQEKYIREWLEERIGSKFNFNLQFYPGKFAVDKRSILPVGDISEIIPDDESDIA 120

Query: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180
           ILEEPEHLTWYHHGRRWK KFK V+GIIHTNYLEYVKREKNGA+EAFLLE+INRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGRRWKKKFKWVVGIIHTNYLEYVKREKNGAVEAFLLEHINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKS+ICNVHGVNPKFLEIG KKMEQ Q+ EQVFGKG YYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSVICNVHGVNPKFLEIGKKKMEQRQDEEQVFGKGVYYIGKMIWS 240

Query: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300
           KGYRELLKLLSD+QKDLAG+E+DLFGNGED+D+VQKA  DLELSV+VH GRDHTDPIFHD
Sbjct: 241 KGYRELLKLLSDHQKDLAGIEVDLFGNGEDSDEVQKAARDLELSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360
           YKVF+NPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNC L D SD FVR +FKA
Sbjct: 301 YKVFVNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCFLCDKSDAFVRGIFKA 360

Query: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420
           LTEEPE LT+ Q HEL+WEAATERFL A+ELDQPL +KPQ+ RSKKFLS+SLQL RKF+D
Sbjct: 361 LTEEPEPLTNVQMHELSWEAATERFLTAAELDQPLAKKPQRTRSKKFLSMSLQLGRKFDD 420

Query: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPPS-KRGSKK 468
           ASAYIHH TLGFEG RR FGAVPGSL+PDEQQCKELG+A PS KRGSKK
Sbjct: 421 ASAYIHHVTLGFEGSRRAFGAVPGSLHPDEQQCKELGIASPSGKRGSKK 469

BLAST of MC05g0332 vs. TAIR 10
Match: AT4G00550.1 (digalactosyl diacylglycerol deficient 2 )

HSP 1 Score: 662.1 bits (1707), Expect = 3.2e-190
Identity = 306/459 (66.67%), Postives = 382/459 (83.22%), Query Frame = 0

Query: 3   RKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSSTTF 62
           ++QHIAIFTTAS+PW+TGTAVNPLFRAAYL+  G RRVTLV+PWL+L+ Q+LVYP+S TF
Sbjct: 5   QEQHIAIFTTASIPWLTGTAVNPLFRAAYLANDGERRVTLVIPWLTLKHQKLVYPNSITF 64

Query: 63  ILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIAIL 122
             PS+QE Y+R+WLEER   +  F ++FYPGKF++DKRSILPVGDIS+ IPD+E+DIA+L
Sbjct: 65  SSPSEQEAYVRQWLEERVSFRLAFEIRFYPGKFAIDKRSILPVGDISDAIPDEEADIAVL 124

Query: 123 EEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIYCH 182
           EEPEHLTW+HHG++WK KF  VIGI+HTNYLEYVKREK G ++AF L+Y+N WVV IYCH
Sbjct: 125 EEPEHLTWFHHGQKWKTKFNYVIGIVHTNYLEYVKREKQGRVKAFFLKYLNSWVVGIYCH 184

Query: 183 KVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWSKG 242
           KVIRLSAATQ+YPKSI+CNVHGVNPKFLEIG +K+EQ +  EQ F KGAYYIGKM+WSKG
Sbjct: 185 KVIRLSAATQEYPKSIVCNVHGVNPKFLEIGLRKLEQQKLQEQPFTKGAYYIGKMVWSKG 244

Query: 243 YRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHDYK 302
           Y+ELLKLL  +QK+LA LE+DL+G+GED++++++A   L+L+V V+ GRDH D +FH+YK
Sbjct: 245 YKELLKLLEKHQKELAELEVDLYGDGEDSEEIKEAARKLDLTVNVYPGRDHADSLFHNYK 304

Query: 303 VFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKALT 362
           VFLNPSTTDVVCTTTAEALAMGK VVCA+H SN+FF+QFPNC  YD+  GFVRA  KAL 
Sbjct: 305 VFLNPSTTDVVCTTTAEALAMGKIVVCANHISNKFFKQFPNCRTYDDGQGFVRATLKALG 364

Query: 363 EEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKK--FLSLSLQLSRKFED 422
           E+P QLT+ QRHEL+WEAAT+RF+K S+L++  + +   N SK+  F S S+ + +  ED
Sbjct: 365 EQPSQLTEQQRHELSWEAATQRFIKVSDLNR--LSRADSNLSKRSVFASSSISVGKNLED 424

Query: 423 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLA 460
            SAYIH    GFE  R  FGA+PGSL PDE+ C++LGL+
Sbjct: 425 MSAYIHFLASGFEASRTAFGAIPGSLQPDEELCRDLGLS 461

BLAST of MC05g0332 vs. TAIR 10
Match: AT3G11670.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 556.2 bits (1432), Expect = 2.5e-158
Identity = 261/460 (56.74%), Postives = 334/460 (72.61%), Query Frame = 0

Query: 2   DRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSSTT 61
           + K+H+AI TTASLPWMTGTAVNPLFRAAYL+    + VTLVVPWL   DQELVYP++ T
Sbjct: 341 ENKRHVAIVTTASLPWMTGTAVNPLFRAAYLAKAAKQSVTLVVPWLCESDQELVYPNNLT 400

Query: 62  FILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIAI 121
           F  P +QE YIRKWLEER G K +F + FYPGKFS ++RSI P GD S+ I   ++DIAI
Sbjct: 401 FSSPEEQESYIRKWLEERIGFKADFKISFYPGKFSKERRSIFPAGDTSQFISSKDADIAI 460

Query: 122 LEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIYC 181
           LEEPEHL WY+HG+RW +KF  V+GI+HTNYLEY+KREKNGAL+AF + ++N WV   YC
Sbjct: 461 LEEPEHLNWYYHGKRWTDKFNHVVGIVHTNYLEYIKREKNGALQAFFVNHVNNWVTRAYC 520

Query: 182 HKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWSK 241
            KV+RLSAATQD PKS++CNVHGVNPKFL IG K  E+   GEQ F KGAY++GKM+W+K
Sbjct: 521 DKVLRLSAATQDLPKSVVCNVHGVNPKFLMIGEKIAEERSRGEQAFSKGAYFLGKMVWAK 580

Query: 242 GYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHDY 301
           GYREL+ L++ ++ +L    +D++GNGEDA +VQ+A +  +L++    GRDH D   H Y
Sbjct: 581 GYRELIDLMAKHKSELGSFNLDVYGNGEDAVEVQRAAKKHDLNLNFLKGRDHADDALHKY 640

Query: 302 KVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKAL 361
           KVF+NPS +DV+CT TAEALAMGKFVVCADHPSNEFFR FPNC+ Y  S+ FV  V +A+
Sbjct: 641 KVFINPSISDVLCTATAEALAMGKFVVCADHPSNEFFRSFPNCLTYKTSEDFVSKVQEAM 700

Query: 362 TEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFEDA 421
           T+EP  LT  Q + L+WEAAT+RF++ S+LD+ L       + +K  S+    +   +  
Sbjct: 701 TKEPLPLTPEQMYNLSWEAATQRFMEYSDLDKILNNGEGGRKMRKSRSVP-SFNEVVDGG 760

Query: 422 SAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLAPP 462
            A+ H+   G +  R   GA P +   D Q CK+L L PP
Sbjct: 761 LAFSHYVLTGNDFLRLCTGATPRTKDYDNQHCKDLNLVPP 799

BLAST of MC05g0332 vs. TAIR 10
Match: AT3G11670.2 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 408.7 bits (1049), Expect = 6.4e-114
Identity = 182/298 (61.07%), Postives = 230/298 (77.18%), Query Frame = 0

Query: 2   DRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSSTT 61
           + K+H+AI TTASLPWMTGTAVNPLFRAAYL+    + VTLVVPWL   DQELVYP++ T
Sbjct: 341 ENKRHVAIVTTASLPWMTGTAVNPLFRAAYLAKAAKQSVTLVVPWLCESDQELVYPNNLT 400

Query: 62  FILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIAI 121
           F  P +QE YIRKWLEER G K +F + FYPGKFS ++RSI P GD S+ I   ++DIAI
Sbjct: 401 FSSPEEQESYIRKWLEERIGFKADFKISFYPGKFSKERRSIFPAGDTSQFISSKDADIAI 460

Query: 122 LEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIYC 181
           LEEPEHL WY+HG+RW +KF  V+GI+HTNYLEY+KREKNGAL+AF + ++N WV   YC
Sbjct: 461 LEEPEHLNWYYHGKRWTDKFNHVVGIVHTNYLEYIKREKNGALQAFFVNHVNNWVTRAYC 520

Query: 182 HKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWSK 241
            KV+RLSAATQD PKS++CNVHGVNPKFL IG K  E+   GEQ F KGAY++GKM+W+K
Sbjct: 521 DKVLRLSAATQDLPKSVVCNVHGVNPKFLMIGEKIAEERSRGEQAFSKGAYFLGKMVWAK 580

Query: 242 GYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFH 300
           GYREL+ L++ ++ +L    +D++GNGEDA +VQ+A +  +L++    GRDH D   H
Sbjct: 581 GYRELIDLMAKHKSELGSFNLDVYGNGEDAVEVQRAAKKHDLNLNFLKGRDHADDALH 638

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6DW754.6e-20272.20Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Glycine max OX=3847 GN=D... [more]
Q6DW731.4e-20171.55Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Lotus japonicus OX=34305... [more]
Q8W1S14.5e-18966.67Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q6DW761.9e-16358.55Digalactosyldiacylglycerol synthase 1, chloroplastic OS=Glycine max OX=3847 GN=D... [more]
Q6DW743.4e-16057.69Digalactosyldiacylglycerol synthase 1, chloroplastic OS=Lotus japonicus OX=34305... [more]
Match NameE-valueIdentityDescription
XP_022147482.10.0100.00digalactosyldiacylglycerol synthase 2, chloroplastic [Momordica charantia] >XP_0... [more]
XP_008438079.10.090.19PREDICTED: digalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis melo] >... [more]
XP_038878183.10.090.41digalactosyldiacylglycerol synthase 2, chloroplastic [Benincasa hispida][more]
XP_004133875.10.089.34digalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis sativus] >KGN56539... [more]
XP_022975105.15.36e-30987.42digalactosyldiacylglycerol synthase 2, chloroplastic [Cucurbita maxima] >XP_0229... [more]
Match NameE-valueIdentityDescription
A0A6J1D1F60.0100.00digalactosyldiacylglycerol synthase 2, chloroplastic OS=Momordica charantia OX=3... [more]
A0A1S3AV600.090.19digalactosyldiacylglycerol synthase 2, chloroplastic OS=Cucumis melo OX=3656 GN=... [more]
A0A5D3D3K60.090.19Digalactosyldiacylglycerol synthase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A0A0L6B20.089.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122630 PE=3 SV=1[more]
A0A6J1IFT42.59e-30987.42digalactosyldiacylglycerol synthase 2, chloroplastic OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
AT4G00550.13.2e-19066.67digalactosyl diacylglycerol deficient 2 [more]
AT3G11670.12.5e-15856.74UDP-Glycosyltransferase superfamily protein [more]
AT3G11670.26.4e-11461.07UDP-Glycosyltransferase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 243..263
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 230..381
e-value: 1.4E-15
score: 59.4
NoneNo IPR availablePFAMPF13692Glyco_trans_1_4coord: 232..362
e-value: 3.3E-9
score: 37.2
NoneNo IPR availablePANTHERPTHR46132:SF1DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTICcoord: 2..461
NoneNo IPR availableCDDcd01635Glycosyltransferase_GTB-typecoord: 166..347
e-value: 2.99834E-19
score: 84.7632
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 7..387
IPR044525Digalactosyldiacylglycerol synthase 1/2PANTHERPTHR46132DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTICcoord: 2..461

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0332.1MC05g0332.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019375 galactolipid biosynthetic process
cellular_component GO:0009707 chloroplast outer membrane
molecular_function GO:0046481 digalactosyldiacylglycerol synthase activity