MC05g0307 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0307 vs. ExPASy Swiss-Prot
Match: Q9LE59 (Polygalacturonate 4-alpha-galacturonosyltransferase OS=Arabidopsis thaliana OX=3702 GN=GAUT1 PE=1 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 5.3e-223
Identity = 367/515 (71.26%), Postives = 441/515 (85.63%), Query Frame = 0

Query: 1   QMVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDST 60
           + +RE+R E +  ELVQ +DDTI+KLEN  +  S+ V S++ GKYS W++ENEN N DS 
Sbjct: 158 RQLREKRREMRANELVQHNDDTILKLENAAIERSKSVDSAVLGKYSIWRRENENDNSDSN 217

Query: 61  IRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKA 120
           IRLMRDQ+IMAR+Y  ++K K K DL +ELQ  LK  +RVLGEA  DA+L  SA +K++A
Sbjct: 218 IRLMRDQVIMARVYSGIAKLKNKNDLLQELQARLKDSQRVLGEATSDADLPRSAHEKLRA 277

Query: 121 MAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSL 180
           M  +L++A+ QL+DCKLVT KLRAML T +E+VR LK+Q+TFL QL  K IP  + CLS+
Sbjct: 278 MGQVLAKAKMQLYDCKLVTGKLRAMLQTADEQVRSLKKQSTFLAQLAAKTIPNPIHCLSM 337

Query: 181 RLTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFH 240
           RLTIDYYLL PE+R FPRSENLE+   +HY LFSDNVLAA+VVVNST+MNAKD S+HVFH
Sbjct: 338 RLTIDYYLLSPEKRKFPRSENLENPNLYHYALFSDNVLAASVVVNSTIMNAKDPSKHVFH 397

Query: 241 LVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAG 300
           LVTD LNFGAM MWFLLNPP +ATI VEN+DEFKWLNSSYCPVLRQL+SAAMREYYFKA 
Sbjct: 398 LVTDKLNFGAMNMWFLLNPPGKATIHVENVDEFKWLNSSYCPVLRQLESAAMREYYFKAD 457

Query: 301 HSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVD 360
           H    +SG+S+LKYRNPKYLSMLNHLRFYLP++YPKL+KILFLDDDI+VQKDLT LW+V+
Sbjct: 458 HP---TSGSSNLKYRNPKYLSMLNHLRFYLPEVYPKLNKILFLDDDIIVQKDLTPLWEVN 517

Query: 361 LEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTG 420
           L GKVNGAVETC  +FHRFDKYLNFSN +IAR F+PNACGWAYGMN+FDLKEWK++++TG
Sbjct: 518 LNGKVNGAVETCGESFHRFDKYLNFSNPHIARNFNPNACGWAYGMNMFDLKEWKKRDITG 577

Query: 421 IYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVI 480
           IYHKWQN+NE+R LW LGTLPPGL+TFYGLT+PL K+WHVLGLGYNPSI+  +IENAAV+
Sbjct: 578 IYHKWQNMNENRTLWKLGTLPPGLITFYGLTHPLNKAWHVLGLGYNPSIDKKDIENAAVV 637

Query: 481 HYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 516
           HYNGNMKPW+E+AM+KYRPYWTKY+ +DHPY+R+C
Sbjct: 638 HYNGNMKPWLELAMSKYRPYWTKYIKFDHPYLRRC 669

BLAST of MC05g0307 vs. ExPASy Swiss-Prot
Match: Q0WQD2 (Probable galacturonosyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=GAUT3 PE=2 SV=2)

HSP 1 Score: 640.2 bits (1650), Expect = 2.0e-182
Identity = 299/519 (57.61%), Postives = 400/519 (77.07%), Query Frame = 0

Query: 1   QMVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDST 60
           Q++R+ER   + +EL++Q+ +T  +++   +  S    +S+ GKYS W+++ E+ N D+ 
Sbjct: 158 QILRQERRGQRTLELIRQEKETDEQMQEAAIQKSMSFENSVIGKYSIWRRDYESPNADAI 217

Query: 61  IRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKA 120
           ++LMRDQIIMA+ Y +++K K   +LY  L     + +RV+G+A  DA+L  SA  + KA
Sbjct: 218 LKLMRDQIIMAKAYANIAKSKNVTNLYVFLMQQCGENKRVIGKATSDADLPSSALDQAKA 277

Query: 121 MAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSL 180
           M   LS A+D+L+DC  + KK RA+L + E KV  LK++ TFL QL  K  P  L CLSL
Sbjct: 278 MGHALSLAKDELYDCHELAKKFRAILQSTERKVDGLKKKGTFLIQLAAKTFPKPLHCLSL 337

Query: 181 RLTIDYYLLPPEQRNFPRSE----NLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSR 240
           +L  DY++L   + +  + +     LE    +HY +FSDNVLA +VVVNSTV+NAK+  R
Sbjct: 338 QLAADYFILGFNEEDAVKEDVSQKKLEDPSLYHYAIFSDNVLATSVVVNSTVLNAKEPQR 397

Query: 241 HVFHLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYY 300
           HVFH+VTD LNFGAM+MWF +N P +ATIQVEN+++FKWLNSSYC VLRQL+SA ++EYY
Sbjct: 398 HVFHIVTDKLNFGAMKMWFRINAPADATIQVENINDFKWLNSSYCSVLRQLESARLKEYY 457

Query: 301 FKAGHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGL 360
           FKA H  + S+GA +LKYRNPKYLSMLNHLRFYLP++YPKL+KILFLDDDIVVQKDL  L
Sbjct: 458 FKANHPSSISAGADNLKYRNPKYLSMLNHLRFYLPEVYPKLEKILFLDDDIVVQKDLAPL 517

Query: 361 WQVDLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEK 420
           W++D++GKVNGAVETC  +FHRFDKYLNFSN  I+  FD  ACGWA+GMN+FDLKEW+++
Sbjct: 518 WEIDMQGKVNGAVETCKESFHRFDKYLNFSNPKISENFDAGACGWAFGMNMFDLKEWRKR 577

Query: 421 NLTGIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIEN 480
           N+TGIYH WQ+LNEDR LW LG+LPPGL+TFY LTY +++SWHVLGLGY+P++N + IEN
Sbjct: 578 NITGIYHYWQDLNEDRTLWKLGSLPPGLITFYNLTYAMDRSWHVLGLGYDPALNQTAIEN 637

Query: 481 AAVIHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 516
           AAV+HYNGN KPW+ +A  KY+PYW+KY++YD+PY+R+C
Sbjct: 638 AAVVHYNGNYKPWLGLAFAKYKPYWSKYVEYDNPYLRRC 676

BLAST of MC05g0307 vs. ExPASy Swiss-Prot
Match: Q93ZX7 (Probable galacturonosyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=GAUT4 PE=2 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 9.1e-167
Identity = 279/508 (54.92%), Postives = 372/508 (73.23%), Query Frame = 0

Query: 9   ENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENEN-KNQDSTIRLMRDQ 68
           EN  ++L QQ  + + +    N   +              KK+  N    D+ +R ++DQ
Sbjct: 123 ENMHVQLTQQTSEKVDEQPEPNAFGA--------------KKDTGNVLMPDAQVRHLKDQ 182

Query: 69  IIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKAMAAILSE 128
           +I A++YLSL   K       EL++ +K+++R L +A+ D++L  +A +K+KAM   L++
Sbjct: 183 LIRAKVYLSLPSAKANAHFVRELRLRIKEVQRALADASKDSDLPKTAIEKLKAMEQTLAK 242

Query: 129 ARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSLRLTIDYY 188
            +    DC  V KKLRAML + +E++R  K+Q  FLTQL  K IP  L CL LRLT DYY
Sbjct: 243 GKQIQDDCSTVVKKLRAMLHSADEQLRVHKKQTMFLTQLTAKTIPKGLHCLPLRLTTDYY 302

Query: 189 LLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFHLVTDNLN 248
            L   ++ FP  E LE  + +HY LFSDNVLA +VVVNST+ NAK   +HVFH+VTD LN
Sbjct: 303 ALNSSEQQFPNQEKLEDTQLYHYALFSDNVLATSVVVNSTITNAKHPLKHVFHIVTDRLN 362

Query: 249 FGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAGHSITRSS 308
           + AMRMWFL NPP +ATIQV+N++EF WLNSSY PVL+QL S +M +YYF+A H    ++
Sbjct: 363 YAAMRMWFLDNPPGKATIQVQNVEEFTWLNSSYSPVLKQLSSRSMIDYYFRAHH----TN 422

Query: 309 GASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVDLEGKVNG 368
             ++LK+RNPKYLS+LNHLRFYLP+I+PKL K+LFLDDDIVVQKDL+GLW VDL+G VNG
Sbjct: 423 SDTNLKFRNPKYLSILNHLRFYLPEIFPKLSKVLFLDDDIVVQKDLSGLWSVDLKGNVNG 482

Query: 369 AVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTGIYHKWQN 428
           AVETC  +FHRFD+YLNFSN  I++ FDP ACGWAYGMN+FDL EWK +N+T +YH+WQ+
Sbjct: 483 AVETCGESFHRFDRYLNFSNPLISKNFDPRACGWAYGMNVFDLDEWKRQNITEVYHRWQD 542

Query: 429 LNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVIHYNGNMK 488
           LN+DR LW LGTLPPGL+TF+  TYPL++ WH+LGLGYNPS+N  +IE AAVIHYNGN+K
Sbjct: 543 LNQDRELWKLGTLPPGLITFWRRTYPLDRKWHILGLGYNPSVNQRDIERAAVIHYNGNLK 602

Query: 489 PWMEMAMTKYRPYWTKYMDYDHPYIRQC 516
           PW+E+ + +YR +W+K++DY+H Y+R+C
Sbjct: 603 PWLEIGIPRYRGFWSKHVDYEHVYLREC 612

BLAST of MC05g0307 vs. ExPASy Swiss-Prot
Match: Q9ZPZ1 (Putative galacturonosyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GAUT2 PE=5 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 6.1e-163
Identity = 285/514 (55.45%), Postives = 365/514 (71.01%), Query Frame = 0

Query: 3   VREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKE-NENKNQDSTI 62
           +R+E+   +  EL+  +DD++ KLE   M  S  V S+  G Y+ WK E    K+ +  +
Sbjct: 40  LRQEKRLERANELM--NDDSLQKLETAAMARSRSVDSAPLGNYTIWKNEYRRGKSFEDML 99

Query: 63  RLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKAM 122
           RLM+DQIIMAR+Y  L+K      L++E++  L K+     E + D +        I+ M
Sbjct: 100 RLMQDQIIMARVYSGLAKFTNNLALHQEIETQLMKL--AWEEESTDIDQEQRVLDSIRDM 159

Query: 123 AAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSLR 182
             IL+ A +QL++CKLVT KLRAML T E+++   +   TFLTQL  K +P A+ CL++R
Sbjct: 160 GQILARAHEQLYECKLVTNKLRAMLQTVEDELENEQTYITFLTQLASKALPDAIHCLTMR 219

Query: 183 LTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFHL 242
           L ++Y+LLP   RNFPR ENLE+ K +HY LFSDNVLAA+VVVNSTVMNA+D SRHVFHL
Sbjct: 220 LNLEYHLLPLPMRNFPRRENLENPKLYHYALFSDNVLAASVVVNSTVMNAQDPSRHVFHL 279

Query: 243 VTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAGH 302
           VTD LNFGAM MWFLLNPP EATI V+  ++F WLNSSY PVL QL+SAAM+++YFK   
Sbjct: 280 VTDKLNFGAMSMWFLLNPPGEATIHVQRFEDFTWLNSSYSPVLSQLESAAMKKFYFKTAR 339

Query: 303 SITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVDL 362
           S +  SG+ +LKYR PKY+SMLNHLRFY+P+I+PKL+KILF+DDD+VVQKDLT LW +DL
Sbjct: 340 SESVESGSENLKYRYPKYMSMLNHLRFYIPRIFPKLEKILFVDDDVVVQKDLTPLWSIDL 399

Query: 363 EGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTGI 422
           +GKVN                           FDP  CGWAYGMNIFDLKEWK+ N+T  
Sbjct: 400 KGKVN-------------------------ENFDPKFCGWAYGMNIFDLKEWKKNNITET 459

Query: 423 YHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVIH 482
           YH WQNLNE+R LW LGTLPPGL+TFY LT PL++ WH+LGLGY+  I++ +IE +AVIH
Sbjct: 460 YHFWQNLNENRTLWKLGTLPPGLITFYNLTQPLQRKWHLLGLGYDKGIDVKKIERSAVIH 519

Query: 483 YNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 516
           YNG+MKPW EM ++KY+PYWTKY ++DHPYI  C
Sbjct: 520 YNGHMKPWTEMGISKYQPYWTKYTNFDHPYIFTC 524

BLAST of MC05g0307 vs. ExPASy Swiss-Prot
Match: Q9LSG3 (Galacturonosyltransferase 8 OS=Arabidopsis thaliana OX=3702 GN=GAUT8 PE=1 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 4.1e-127
Identity = 210/415 (50.60%), Postives = 298/415 (71.81%), Query Frame = 0

Query: 103 EANIDAELHYSAPKKIKAMAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTF 162
           E ++  +      ++IK    +++EA++  FD +L  +KL+  +    E++   K+Q  F
Sbjct: 143 EESVLRQFEKEVKERIKMTRQVIAEAKES-FDNQLKIQKLKDTIFAVNEQLTNAKKQGAF 202

Query: 163 LTQLGVKGIPGALRCLSLRLTIDYYLLPPE--QRNFPRSENLESKKFHHYGLFSDNVLAA 222
            + +  K IP  L CL++RL  +    P +       R   LE    +HY +FSDNV+AA
Sbjct: 203 SSLIAAKSIPKGLHCLAMRLMEERIAHPEKYTDEGKDRPRELEDPNLYHYAIFSDNVIAA 262

Query: 223 AVVVNSTVMNAKDSSRHVFHLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSY 282
           +VVVNS V NAK+  +HVFH+VTD +N GAM++ F L   K A ++V+ ++++ +LNSSY
Sbjct: 263 SVVVNSAVKNAKEPWKHVFHVVTDKMNLGAMQVMFKLKEYKGAHVEVKAVEDYTFLNSSY 322

Query: 283 CPVLRQLQSAAMREYYFKAGHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKI 342
            PVL+QL+SA ++++YF+       +   +++K+RNPKYLS+LNHLRFYLP++YPKL +I
Sbjct: 323 VPVLKQLESANLQKFYFE-NKLENATKDTTNMKFRNPKYLSILNHLRFYLPEMYPKLHRI 382

Query: 343 LFLDDDIVVQKDLTGLWQVDLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACG 402
           LFLDDD+VVQKDLTGLW++D++GKVNGAVETC  +FHR+ +Y+NFS+  I  +F+P AC 
Sbjct: 383 LFLDDDVVVQKDLTGLWEIDMDGKVNGAVETCFGSFHRYAQYMNFSHPLIKEKFNPKACA 442

Query: 403 WAYGMNIFDLKEWKEKNLTGIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHV 462
           WAYGMN FDL  W+ +  T  YH WQNLNE+R LW LGTLPPGL+TFY  T PL+KSWHV
Sbjct: 443 WAYGMNFFDLDAWRREKCTEEYHYWQNLNENRALWKLGTLPPGLITFYSTTKPLDKSWHV 502

Query: 463 LGLGYNPSINLSEIENAAVIHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 516
           LGLGYNPSI++ EI NAAV+H+NGNMKPW+++AM ++RP WTK++DYD  +++ C
Sbjct: 503 LGLGYNPSISMDEIRNAAVVHFNGNMKPWLDIAMNQFRPLWTKHVDYDLEFVQAC 555

BLAST of MC05g0307 vs. NCBI nr
Match: XP_022147397.1 (polygalacturonate 4-alpha-galacturonosyltransferase-like [Momordica charantia])

HSP 1 Score: 1043 bits (2696), Expect = 0.0
Identity = 513/514 (99.81%), Postives = 513/514 (99.81%), Query Frame = 0

Query: 2   MVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDSTI 61
           MVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDSTI
Sbjct: 139 MVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDSTI 198

Query: 62  RLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKAM 121
           RLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKAM
Sbjct: 199 RLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKAM 258

Query: 122 AAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSLR 181
           AAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSLR
Sbjct: 259 AAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSLR 318

Query: 182 LTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFHL 241
           L IDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFHL
Sbjct: 319 LXIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFHL 378

Query: 242 VTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAGH 301
           VTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAGH
Sbjct: 379 VTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAGH 438

Query: 302 SITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVDL 361
           SITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVDL
Sbjct: 439 SITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVDL 498

Query: 362 EGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTGI 421
           EGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTGI
Sbjct: 499 EGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTGI 558

Query: 422 YHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVIH 481
           YHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVIH
Sbjct: 559 YHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVIH 618

Query: 482 YNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 515
           YNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC
Sbjct: 619 YNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 652

BLAST of MC05g0307 vs. NCBI nr
Match: XP_038877503.1 (polygalacturonate 4-alpha-galacturonosyltransferase-like isoform X3 [Benincasa hispida])

HSP 1 Score: 917 bits (2369), Expect = 0.0
Identity = 446/516 (86.43%), Postives = 476/516 (92.25%), Query Frame = 0

Query: 2   MVREERYENQF--MELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDS 61
           + REER ENQ   MEL  Q +DT VKLEN NM AS+LV SSI GKYSTWKKEN+NKNQDS
Sbjct: 133 IAREERQENQLLEMELDLQGEDTAVKLENTNMQASDLVDSSILGKYSTWKKENDNKNQDS 192

Query: 62  TIRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIK 121
            IRLMRDQIIMARIYLS+SKKKKK DL EELQI LK+ RRVLGEANIDAELHYSAP+KIK
Sbjct: 193 IIRLMRDQIIMARIYLSISKKKKKLDLIEELQICLKRSRRVLGEANIDAELHYSAPQKIK 252

Query: 122 AMAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLS 181
           AMA +LS+ARDQLFDCKLV +KLRAMLLTEEEK+RRLKRQNTFLTQLGVKGIPG +RCLS
Sbjct: 253 AMAGVLSKARDQLFDCKLVIRKLRAMLLTEEEKIRRLKRQNTFLTQLGVKGIPGGIRCLS 312

Query: 182 LRLTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVF 241
           LRLTIDYYLLPPE+RNFPRS NL++ KFHHY LF+DNV+AAAVVVNST+MNAKDSS+HVF
Sbjct: 313 LRLTIDYYLLPPEKRNFPRSVNLQNPKFHHYALFTDNVMAAAVVVNSTIMNAKDSSKHVF 372

Query: 242 HLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKA 301
           HLVTDNLN GA++MWFLLNPP+EATI VENLDE KWLNSSYCPVLRQL SAAMREYYFK 
Sbjct: 373 HLVTDNLNIGALKMWFLLNPPEEATIHVENLDELKWLNSSYCPVLRQLNSAAMREYYFKT 432

Query: 302 GHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQV 361
           GHS T SS ASSLKYRNPKYLSMLNHLRFY+PQIYPKLDKILFLDDD+VVQKDLTGLW V
Sbjct: 433 GHSTTPSSSASSLKYRNPKYLSMLNHLRFYIPQIYPKLDKILFLDDDVVVQKDLTGLWLV 492

Query: 362 DLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLT 421
           DLEGKVNGAVETCV NFHRFDKYLNFSN YIAREFDPNACGWAYGMNIFDLKEWK++NLT
Sbjct: 493 DLEGKVNGAVETCVENFHRFDKYLNFSNPYIAREFDPNACGWAYGMNIFDLKEWKKRNLT 552

Query: 422 GIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAV 481
           G+YH WQNLNEDRLLW LGTLPPGLMTFYGLTY L KSWHVLGLGYNPSIN SEIENAAV
Sbjct: 553 GVYHTWQNLNEDRLLWKLGTLPPGLMTFYGLTYSLNKSWHVLGLGYNPSINPSEIENAAV 612

Query: 482 IHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 515
           IHYNGNMKPW+EMAMTKYRPYWTKY+DYDHPY+RQC
Sbjct: 613 IHYNGNMKPWLEMAMTKYRPYWTKYIDYDHPYLRQC 648

BLAST of MC05g0307 vs. NCBI nr
Match: XP_038877493.1 (polygalacturonate 4-alpha-galacturonosyltransferase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 917 bits (2369), Expect = 0.0
Identity = 446/516 (86.43%), Postives = 476/516 (92.25%), Query Frame = 0

Query: 2   MVREERYENQF--MELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDS 61
           + REER ENQ   MEL  Q +DT VKLEN NM AS+LV SSI GKYSTWKKEN+NKNQDS
Sbjct: 134 IAREERQENQLLEMELDLQGEDTAVKLENTNMQASDLVDSSILGKYSTWKKENDNKNQDS 193

Query: 62  TIRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIK 121
            IRLMRDQIIMARIYLS+SKKKKK DL EELQI LK+ RRVLGEANIDAELHYSAP+KIK
Sbjct: 194 IIRLMRDQIIMARIYLSISKKKKKLDLIEELQICLKRSRRVLGEANIDAELHYSAPQKIK 253

Query: 122 AMAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLS 181
           AMA +LS+ARDQLFDCKLV +KLRAMLLTEEEK+RRLKRQNTFLTQLGVKGIPG +RCLS
Sbjct: 254 AMAGVLSKARDQLFDCKLVIRKLRAMLLTEEEKIRRLKRQNTFLTQLGVKGIPGGIRCLS 313

Query: 182 LRLTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVF 241
           LRLTIDYYLLPPE+RNFPRS NL++ KFHHY LF+DNV+AAAVVVNST+MNAKDSS+HVF
Sbjct: 314 LRLTIDYYLLPPEKRNFPRSVNLQNPKFHHYALFTDNVMAAAVVVNSTIMNAKDSSKHVF 373

Query: 242 HLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKA 301
           HLVTDNLN GA++MWFLLNPP+EATI VENLDE KWLNSSYCPVLRQL SAAMREYYFK 
Sbjct: 374 HLVTDNLNIGALKMWFLLNPPEEATIHVENLDELKWLNSSYCPVLRQLNSAAMREYYFKT 433

Query: 302 GHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQV 361
           GHS T SS ASSLKYRNPKYLSMLNHLRFY+PQIYPKLDKILFLDDD+VVQKDLTGLW V
Sbjct: 434 GHSTTPSSSASSLKYRNPKYLSMLNHLRFYIPQIYPKLDKILFLDDDVVVQKDLTGLWLV 493

Query: 362 DLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLT 421
           DLEGKVNGAVETCV NFHRFDKYLNFSN YIAREFDPNACGWAYGMNIFDLKEWK++NLT
Sbjct: 494 DLEGKVNGAVETCVENFHRFDKYLNFSNPYIAREFDPNACGWAYGMNIFDLKEWKKRNLT 553

Query: 422 GIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAV 481
           G+YH WQNLNEDRLLW LGTLPPGLMTFYGLTY L KSWHVLGLGYNPSIN SEIENAAV
Sbjct: 554 GVYHTWQNLNEDRLLWKLGTLPPGLMTFYGLTYSLNKSWHVLGLGYNPSINPSEIENAAV 613

Query: 482 IHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 515
           IHYNGNMKPW+EMAMTKYRPYWTKY+DYDHPY+RQC
Sbjct: 614 IHYNGNMKPWLEMAMTKYRPYWTKYIDYDHPYLRQC 649

BLAST of MC05g0307 vs. NCBI nr
Match: XP_038877483.1 (polygalacturonate 4-alpha-galacturonosyltransferase-like isoform X1 [Benincasa hispida] >XP_038877489.1 polygalacturonate 4-alpha-galacturonosyltransferase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 917 bits (2369), Expect = 0.0
Identity = 446/516 (86.43%), Postives = 476/516 (92.25%), Query Frame = 0

Query: 2   MVREERYENQF--MELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDS 61
           + REER ENQ   MEL  Q +DT VKLEN NM AS+LV SSI GKYSTWKKEN+NKNQDS
Sbjct: 176 IAREERQENQLLEMELDLQGEDTAVKLENTNMQASDLVDSSILGKYSTWKKENDNKNQDS 235

Query: 62  TIRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIK 121
            IRLMRDQIIMARIYLS+SKKKKK DL EELQI LK+ RRVLGEANIDAELHYSAP+KIK
Sbjct: 236 IIRLMRDQIIMARIYLSISKKKKKLDLIEELQICLKRSRRVLGEANIDAELHYSAPQKIK 295

Query: 122 AMAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLS 181
           AMA +LS+ARDQLFDCKLV +KLRAMLLTEEEK+RRLKRQNTFLTQLGVKGIPG +RCLS
Sbjct: 296 AMAGVLSKARDQLFDCKLVIRKLRAMLLTEEEKIRRLKRQNTFLTQLGVKGIPGGIRCLS 355

Query: 182 LRLTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVF 241
           LRLTIDYYLLPPE+RNFPRS NL++ KFHHY LF+DNV+AAAVVVNST+MNAKDSS+HVF
Sbjct: 356 LRLTIDYYLLPPEKRNFPRSVNLQNPKFHHYALFTDNVMAAAVVVNSTIMNAKDSSKHVF 415

Query: 242 HLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKA 301
           HLVTDNLN GA++MWFLLNPP+EATI VENLDE KWLNSSYCPVLRQL SAAMREYYFK 
Sbjct: 416 HLVTDNLNIGALKMWFLLNPPEEATIHVENLDELKWLNSSYCPVLRQLNSAAMREYYFKT 475

Query: 302 GHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQV 361
           GHS T SS ASSLKYRNPKYLSMLNHLRFY+PQIYPKLDKILFLDDD+VVQKDLTGLW V
Sbjct: 476 GHSTTPSSSASSLKYRNPKYLSMLNHLRFYIPQIYPKLDKILFLDDDVVVQKDLTGLWLV 535

Query: 362 DLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLT 421
           DLEGKVNGAVETCV NFHRFDKYLNFSN YIAREFDPNACGWAYGMNIFDLKEWK++NLT
Sbjct: 536 DLEGKVNGAVETCVENFHRFDKYLNFSNPYIAREFDPNACGWAYGMNIFDLKEWKKRNLT 595

Query: 422 GIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAV 481
           G+YH WQNLNEDRLLW LGTLPPGLMTFYGLTY L KSWHVLGLGYNPSIN SEIENAAV
Sbjct: 596 GVYHTWQNLNEDRLLWKLGTLPPGLMTFYGLTYSLNKSWHVLGLGYNPSINPSEIENAAV 655

Query: 482 IHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 515
           IHYNGNMKPW+EMAMTKYRPYWTKY+DYDHPY+RQC
Sbjct: 656 IHYNGNMKPWLEMAMTKYRPYWTKYIDYDHPYLRQC 691

BLAST of MC05g0307 vs. NCBI nr
Match: XP_023526600.1 (polygalacturonate 4-alpha-galacturonosyltransferase-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 915 bits (2365), Expect = 0.0
Identity = 448/516 (86.82%), Postives = 479/516 (92.83%), Query Frame = 0

Query: 2   MVREERYENQFMELVQQ--DDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDS 61
           + REER ENQ +EL     ++DTIVKLEN NM ASELV SSI GKYS WKKEN+NKNQDS
Sbjct: 132 IAREERNENQLLELDLDPPNEDTIVKLENANMQASELVDSSITGKYSKWKKENKNKNQDS 191

Query: 62  TIRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIK 121
           TIRLMRDQIIMARIYLS+S+KK+K DL +ELQI LK+ R VLGEANIDAELHYSAPKKIK
Sbjct: 192 TIRLMRDQIIMARIYLSISEKKRKLDLVQELQICLKRSRGVLGEANIDAELHYSAPKKIK 251

Query: 122 AMAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLS 181
           AMA +LS+ARDQLFDCKLVT+KLRAMLLTEEEKVRRLKRQNTFL QLGVKGIPG +RCLS
Sbjct: 252 AMADVLSKARDQLFDCKLVTRKLRAMLLTEEEKVRRLKRQNTFLIQLGVKGIPGGIRCLS 311

Query: 182 LRLTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVF 241
           LRLTIDYYLLPPE+RNFPRSENLE  +FHHY L +DNVLAAAVVVNSTVMNAKDSS+HVF
Sbjct: 312 LRLTIDYYLLPPERRNFPRSENLEDPRFHHYALSTDNVLAAAVVVNSTVMNAKDSSKHVF 371

Query: 242 HLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKA 301
           HLVTD+LNFGA++MWFLLNPP+EATI VENLDE KWLNSSYCPVLRQL SAAMREYYFKA
Sbjct: 372 HLVTDSLNFGALKMWFLLNPPEEATIHVENLDELKWLNSSYCPVLRQLNSAAMREYYFKA 431

Query: 302 GHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQV 361
           GHS TRSS AS+LKYRNPKYLSMLNHLRFYLPQI+P+LDKILFLDDDIVVQKDLTGLW V
Sbjct: 432 GHSTTRSSSASNLKYRNPKYLSMLNHLRFYLPQIFPELDKILFLDDDIVVQKDLTGLWLV 491

Query: 362 DLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLT 421
           DLEGKVNGAVETCV NFHRFDKYLNFSNS+IAREFDPNACGWAYGMNIFDLKEWK++NLT
Sbjct: 492 DLEGKVNGAVETCVENFHRFDKYLNFSNSFIAREFDPNACGWAYGMNIFDLKEWKKRNLT 551

Query: 422 GIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAV 481
           G+YH WQNLNEDRLLW LGTLPPGLMTFYGLTY L KSWHVLGLGYNPSIN SEIENAAV
Sbjct: 552 GVYHTWQNLNEDRLLWKLGTLPPGLMTFYGLTYSLNKSWHVLGLGYNPSINPSEIENAAV 611

Query: 482 IHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 515
           IHYNGNMKPWMEMAMTKYRPYWTKY+DYDHPY+RQC
Sbjct: 612 IHYNGNMKPWMEMAMTKYRPYWTKYIDYDHPYLRQC 647

BLAST of MC05g0307 vs. ExPASy TrEMBL
Match: A0A6J1D293 (Hexosyltransferase OS=Momordica charantia OX=3673 GN=LOC111016337 PE=3 SV=1)

HSP 1 Score: 1043 bits (2696), Expect = 0.0
Identity = 513/514 (99.81%), Postives = 513/514 (99.81%), Query Frame = 0

Query: 2   MVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDSTI 61
           MVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDSTI
Sbjct: 139 MVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDSTI 198

Query: 62  RLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKAM 121
           RLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKAM
Sbjct: 199 RLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKAM 258

Query: 122 AAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSLR 181
           AAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSLR
Sbjct: 259 AAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSLR 318

Query: 182 LTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFHL 241
           L IDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFHL
Sbjct: 319 LXIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFHL 378

Query: 242 VTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAGH 301
           VTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAGH
Sbjct: 379 VTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAGH 438

Query: 302 SITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVDL 361
           SITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVDL
Sbjct: 439 SITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVDL 498

Query: 362 EGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTGI 421
           EGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTGI
Sbjct: 499 EGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTGI 558

Query: 422 YHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVIH 481
           YHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVIH
Sbjct: 559 YHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVIH 618

Query: 482 YNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 515
           YNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC
Sbjct: 619 YNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 652

BLAST of MC05g0307 vs. ExPASy TrEMBL
Match: A0A6J1IYS2 (Hexosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479673 PE=3 SV=1)

HSP 1 Score: 914 bits (2361), Expect = 0.0
Identity = 447/516 (86.63%), Postives = 480/516 (93.02%), Query Frame = 0

Query: 2   MVREERYENQFMELVQQ--DDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDS 61
           + REER+ENQ +EL     ++DTIVKLEN NM ASELV SSI GKYS WKKEN+NKNQDS
Sbjct: 133 IAREERHENQLLELDLDPPNEDTIVKLENANMQASELVDSSIIGKYSKWKKENQNKNQDS 192

Query: 62  TIRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIK 121
           TIRLMRDQIIMARIYLS+S+KK+K DL +ELQI LK+ R VLGEANIDAELHYSAPKKIK
Sbjct: 193 TIRLMRDQIIMARIYLSISEKKRKHDLVQELQICLKRSRGVLGEANIDAELHYSAPKKIK 252

Query: 122 AMAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLS 181
           AMA +LS+ARDQLFDCKLVT+KLRAMLLTEEEKVRRLKRQNTFL QLGVKGIPG +RCLS
Sbjct: 253 AMADVLSKARDQLFDCKLVTRKLRAMLLTEEEKVRRLKRQNTFLIQLGVKGIPGGIRCLS 312

Query: 182 LRLTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVF 241
           LRLTIDYYLLPPE+RNFPRSENLE  +FHHY L +DNVLAAAVVVNSTVMNAKDSS+HVF
Sbjct: 313 LRLTIDYYLLPPERRNFPRSENLEDPRFHHYALSTDNVLAAAVVVNSTVMNAKDSSKHVF 372

Query: 242 HLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKA 301
           HLVTD+LNFGA++MWFLLNPP+EATI VENLDE KWLNSSYCPVLRQL SAAMREYYFKA
Sbjct: 373 HLVTDSLNFGALKMWFLLNPPEEATIHVENLDELKWLNSSYCPVLRQLNSAAMREYYFKA 432

Query: 302 GHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQV 361
           GHS TRSS AS+LKYRNPKYLSMLNHLRFYLPQI+P+LDKILFLDDDIVVQKDLTGLW V
Sbjct: 433 GHSTTRSSSASNLKYRNPKYLSMLNHLRFYLPQIFPELDKILFLDDDIVVQKDLTGLWLV 492

Query: 362 DLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLT 421
           DLEGKVNGAVETCV NFHRFDKYLNFSNS+IAREFDPNACGWAYGMNIFDLKEWK++NLT
Sbjct: 493 DLEGKVNGAVETCVENFHRFDKYLNFSNSFIAREFDPNACGWAYGMNIFDLKEWKKRNLT 552

Query: 422 GIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAV 481
           G+YH WQNLNEDRLLW LGTLPPGLMTFYGLTY L KSWHVLGLGYNPSIN SEIENAAV
Sbjct: 553 GVYHTWQNLNEDRLLWKLGTLPPGLMTFYGLTYSLNKSWHVLGLGYNPSINPSEIENAAV 612

Query: 482 IHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 515
           IHYNGNMKPWMEMAMTKYRPYWTKY+DYD+PY+RQC
Sbjct: 613 IHYNGNMKPWMEMAMTKYRPYWTKYIDYDNPYLRQC 648

BLAST of MC05g0307 vs. ExPASy TrEMBL
Match: A0A6J1EEY0 (Hexosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111431921 PE=3 SV=1)

HSP 1 Score: 912 bits (2358), Expect = 0.0
Identity = 447/516 (86.63%), Postives = 478/516 (92.64%), Query Frame = 0

Query: 2   MVREERYENQFMELV--QQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDS 61
           + REER ENQ +EL     ++DTIVKLEN NM ASELV SSI GKYS WKKEN+NKNQDS
Sbjct: 175 IAREERNENQLLELDFDPPNEDTIVKLENANMQASELVDSSITGKYSKWKKENKNKNQDS 234

Query: 62  TIRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIK 121
           TIRLMRDQIIMARIYLS+S+KK+K DL +ELQI LK+ R VLGEANIDAELHYSAPKKIK
Sbjct: 235 TIRLMRDQIIMARIYLSISEKKRKLDLVQELQICLKRSRGVLGEANIDAELHYSAPKKIK 294

Query: 122 AMAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLS 181
           AMA +LS+ARDQLFDCKLVT+KLRAMLLTEEEKVRRLKRQNTFL QLGVKGIPG +RCLS
Sbjct: 295 AMADVLSKARDQLFDCKLVTRKLRAMLLTEEEKVRRLKRQNTFLIQLGVKGIPGGIRCLS 354

Query: 182 LRLTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVF 241
           LRLTIDYYLLPPE+RNFPRSENLE  +FHHY L +DNVLAAAVVVNSTVMNAKDSS+HVF
Sbjct: 355 LRLTIDYYLLPPERRNFPRSENLEDPRFHHYALSTDNVLAAAVVVNSTVMNAKDSSKHVF 414

Query: 242 HLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKA 301
           HLVTD+LNFGA++MWFLLNPP+EATI VENLDE KWLNSSYCPVLRQL SAAMREYYFKA
Sbjct: 415 HLVTDSLNFGALKMWFLLNPPEEATIHVENLDELKWLNSSYCPVLRQLNSAAMREYYFKA 474

Query: 302 GHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQV 361
           GHS TRSS AS+LKYRNPKYLSMLNHLRFYLPQI+P+LDKILFLDDDIVVQKDLTGLW V
Sbjct: 475 GHSTTRSSSASNLKYRNPKYLSMLNHLRFYLPQIFPELDKILFLDDDIVVQKDLTGLWLV 534

Query: 362 DLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLT 421
           DLEGKVNGAVETCV NFHRFDKYLNFSNS+IA EFDPNACGWAYGMNIFDLKEWK++NLT
Sbjct: 535 DLEGKVNGAVETCVENFHRFDKYLNFSNSFIAGEFDPNACGWAYGMNIFDLKEWKKRNLT 594

Query: 422 GIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAV 481
           G+YH WQNLNEDRLLW LGTLPPGLMTFYGLTY L KSWHVLGLGYNPSIN SEIENAAV
Sbjct: 595 GVYHTWQNLNEDRLLWKLGTLPPGLMTFYGLTYSLNKSWHVLGLGYNPSINPSEIENAAV 654

Query: 482 IHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 515
           IHYNGNMKPWMEMAMTKYRPYWTKY+DYDHPY+RQC
Sbjct: 655 IHYNGNMKPWMEMAMTKYRPYWTKYIDYDHPYLRQC 690

BLAST of MC05g0307 vs. ExPASy TrEMBL
Match: A0A6J1EEY5 (Hexosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111431921 PE=3 SV=1)

HSP 1 Score: 912 bits (2358), Expect = 0.0
Identity = 447/516 (86.63%), Postives = 478/516 (92.64%), Query Frame = 0

Query: 2   MVREERYENQFMELV--QQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDS 61
           + REER ENQ +EL     ++DTIVKLEN NM ASELV SSI GKYS WKKEN+NKNQDS
Sbjct: 69  IAREERNENQLLELDFDPPNEDTIVKLENANMQASELVDSSITGKYSKWKKENKNKNQDS 128

Query: 62  TIRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIK 121
           TIRLMRDQIIMARIYLS+S+KK+K DL +ELQI LK+ R VLGEANIDAELHYSAPKKIK
Sbjct: 129 TIRLMRDQIIMARIYLSISEKKRKLDLVQELQICLKRSRGVLGEANIDAELHYSAPKKIK 188

Query: 122 AMAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLS 181
           AMA +LS+ARDQLFDCKLVT+KLRAMLLTEEEKVRRLKRQNTFL QLGVKGIPG +RCLS
Sbjct: 189 AMADVLSKARDQLFDCKLVTRKLRAMLLTEEEKVRRLKRQNTFLIQLGVKGIPGGIRCLS 248

Query: 182 LRLTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVF 241
           LRLTIDYYLLPPE+RNFPRSENLE  +FHHY L +DNVLAAAVVVNSTVMNAKDSS+HVF
Sbjct: 249 LRLTIDYYLLPPERRNFPRSENLEDPRFHHYALSTDNVLAAAVVVNSTVMNAKDSSKHVF 308

Query: 242 HLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKA 301
           HLVTD+LNFGA++MWFLLNPP+EATI VENLDE KWLNSSYCPVLRQL SAAMREYYFKA
Sbjct: 309 HLVTDSLNFGALKMWFLLNPPEEATIHVENLDELKWLNSSYCPVLRQLNSAAMREYYFKA 368

Query: 302 GHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQV 361
           GHS TRSS AS+LKYRNPKYLSMLNHLRFYLPQI+P+LDKILFLDDDIVVQKDLTGLW V
Sbjct: 369 GHSTTRSSSASNLKYRNPKYLSMLNHLRFYLPQIFPELDKILFLDDDIVVQKDLTGLWLV 428

Query: 362 DLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLT 421
           DLEGKVNGAVETCV NFHRFDKYLNFSNS+IA EFDPNACGWAYGMNIFDLKEWK++NLT
Sbjct: 429 DLEGKVNGAVETCVENFHRFDKYLNFSNSFIAGEFDPNACGWAYGMNIFDLKEWKKRNLT 488

Query: 422 GIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAV 481
           G+YH WQNLNEDRLLW LGTLPPGLMTFYGLTY L KSWHVLGLGYNPSIN SEIENAAV
Sbjct: 489 GVYHTWQNLNEDRLLWKLGTLPPGLMTFYGLTYSLNKSWHVLGLGYNPSINPSEIENAAV 548

Query: 482 IHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 515
           IHYNGNMKPWMEMAMTKYRPYWTKY+DYDHPY+RQC
Sbjct: 549 IHYNGNMKPWMEMAMTKYRPYWTKYIDYDHPYLRQC 584

BLAST of MC05g0307 vs. ExPASy TrEMBL
Match: A0A6J1E937 (Hexosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111431921 PE=3 SV=1)

HSP 1 Score: 912 bits (2358), Expect = 0.0
Identity = 447/516 (86.63%), Postives = 478/516 (92.64%), Query Frame = 0

Query: 2   MVREERYENQFMELV--QQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDS 61
           + REER ENQ +EL     ++DTIVKLEN NM ASELV SSI GKYS WKKEN+NKNQDS
Sbjct: 108 IAREERNENQLLELDFDPPNEDTIVKLENANMQASELVDSSITGKYSKWKKENKNKNQDS 167

Query: 62  TIRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIK 121
           TIRLMRDQIIMARIYLS+S+KK+K DL +ELQI LK+ R VLGEANIDAELHYSAPKKIK
Sbjct: 168 TIRLMRDQIIMARIYLSISEKKRKLDLVQELQICLKRSRGVLGEANIDAELHYSAPKKIK 227

Query: 122 AMAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLS 181
           AMA +LS+ARDQLFDCKLVT+KLRAMLLTEEEKVRRLKRQNTFL QLGVKGIPG +RCLS
Sbjct: 228 AMADVLSKARDQLFDCKLVTRKLRAMLLTEEEKVRRLKRQNTFLIQLGVKGIPGGIRCLS 287

Query: 182 LRLTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVF 241
           LRLTIDYYLLPPE+RNFPRSENLE  +FHHY L +DNVLAAAVVVNSTVMNAKDSS+HVF
Sbjct: 288 LRLTIDYYLLPPERRNFPRSENLEDPRFHHYALSTDNVLAAAVVVNSTVMNAKDSSKHVF 347

Query: 242 HLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKA 301
           HLVTD+LNFGA++MWFLLNPP+EATI VENLDE KWLNSSYCPVLRQL SAAMREYYFKA
Sbjct: 348 HLVTDSLNFGALKMWFLLNPPEEATIHVENLDELKWLNSSYCPVLRQLNSAAMREYYFKA 407

Query: 302 GHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQV 361
           GHS TRSS AS+LKYRNPKYLSMLNHLRFYLPQI+P+LDKILFLDDDIVVQKDLTGLW V
Sbjct: 408 GHSTTRSSSASNLKYRNPKYLSMLNHLRFYLPQIFPELDKILFLDDDIVVQKDLTGLWLV 467

Query: 362 DLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLT 421
           DLEGKVNGAVETCV NFHRFDKYLNFSNS+IA EFDPNACGWAYGMNIFDLKEWK++NLT
Sbjct: 468 DLEGKVNGAVETCVENFHRFDKYLNFSNSFIAGEFDPNACGWAYGMNIFDLKEWKKRNLT 527

Query: 422 GIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAV 481
           G+YH WQNLNEDRLLW LGTLPPGLMTFYGLTY L KSWHVLGLGYNPSIN SEIENAAV
Sbjct: 528 GVYHTWQNLNEDRLLWKLGTLPPGLMTFYGLTYSLNKSWHVLGLGYNPSINPSEIENAAV 587

Query: 482 IHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 515
           IHYNGNMKPWMEMAMTKYRPYWTKY+DYDHPY+RQC
Sbjct: 588 IHYNGNMKPWMEMAMTKYRPYWTKYIDYDHPYLRQC 623

BLAST of MC05g0307 vs. TAIR 10
Match: AT3G61130.1 (galacturonosyltransferase 1 )

HSP 1 Score: 775.0 bits (2000), Expect = 3.7e-224
Identity = 367/515 (71.26%), Postives = 441/515 (85.63%), Query Frame = 0

Query: 1   QMVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDST 60
           + +RE+R E +  ELVQ +DDTI+KLEN  +  S+ V S++ GKYS W++ENEN N DS 
Sbjct: 158 RQLREKRREMRANELVQHNDDTILKLENAAIERSKSVDSAVLGKYSIWRRENENDNSDSN 217

Query: 61  IRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKA 120
           IRLMRDQ+IMAR+Y  ++K K K DL +ELQ  LK  +RVLGEA  DA+L  SA +K++A
Sbjct: 218 IRLMRDQVIMARVYSGIAKLKNKNDLLQELQARLKDSQRVLGEATSDADLPRSAHEKLRA 277

Query: 121 MAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSL 180
           M  +L++A+ QL+DCKLVT KLRAML T +E+VR LK+Q+TFL QL  K IP  + CLS+
Sbjct: 278 MGQVLAKAKMQLYDCKLVTGKLRAMLQTADEQVRSLKKQSTFLAQLAAKTIPNPIHCLSM 337

Query: 181 RLTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFH 240
           RLTIDYYLL PE+R FPRSENLE+   +HY LFSDNVLAA+VVVNST+MNAKD S+HVFH
Sbjct: 338 RLTIDYYLLSPEKRKFPRSENLENPNLYHYALFSDNVLAASVVVNSTIMNAKDPSKHVFH 397

Query: 241 LVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAG 300
           LVTD LNFGAM MWFLLNPP +ATI VEN+DEFKWLNSSYCPVLRQL+SAAMREYYFKA 
Sbjct: 398 LVTDKLNFGAMNMWFLLNPPGKATIHVENVDEFKWLNSSYCPVLRQLESAAMREYYFKAD 457

Query: 301 HSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVD 360
           H    +SG+S+LKYRNPKYLSMLNHLRFYLP++YPKL+KILFLDDDI+VQKDLT LW+V+
Sbjct: 458 HP---TSGSSNLKYRNPKYLSMLNHLRFYLPEVYPKLNKILFLDDDIIVQKDLTPLWEVN 517

Query: 361 LEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTG 420
           L GKVNGAVETC  +FHRFDKYLNFSN +IAR F+PNACGWAYGMN+FDLKEWK++++TG
Sbjct: 518 LNGKVNGAVETCGESFHRFDKYLNFSNPHIARNFNPNACGWAYGMNMFDLKEWKKRDITG 577

Query: 421 IYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVI 480
           IYHKWQN+NE+R LW LGTLPPGL+TFYGLT+PL K+WHVLGLGYNPSI+  +IENAAV+
Sbjct: 578 IYHKWQNMNENRTLWKLGTLPPGLITFYGLTHPLNKAWHVLGLGYNPSIDKKDIENAAVV 637

Query: 481 HYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 516
           HYNGNMKPW+E+AM+KYRPYWTKY+ +DHPY+R+C
Sbjct: 638 HYNGNMKPWLELAMSKYRPYWTKYIKFDHPYLRRC 669

BLAST of MC05g0307 vs. TAIR 10
Match: AT4G38270.1 (galacturonosyltransferase 3 )

HSP 1 Score: 640.2 bits (1650), Expect = 1.4e-183
Identity = 299/519 (57.61%), Postives = 400/519 (77.07%), Query Frame = 0

Query: 1   QMVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDST 60
           Q++R+ER   + +EL++Q+ +T  +++   +  S    +S+ GKYS W+++ E+ N D+ 
Sbjct: 158 QILRQERRGQRTLELIRQEKETDEQMQEAAIQKSMSFENSVIGKYSIWRRDYESPNADAI 217

Query: 61  IRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKA 120
           ++LMRDQIIMA+ Y +++K K   +LY  L     + +RV+G+A  DA+L  SA  + KA
Sbjct: 218 LKLMRDQIIMAKAYANIAKSKNVTNLYVFLMQQCGENKRVIGKATSDADLPSSALDQAKA 277

Query: 121 MAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSL 180
           M   LS A+D+L+DC  + KK RA+L + E KV  LK++ TFL QL  K  P  L CLSL
Sbjct: 278 MGHALSLAKDELYDCHELAKKFRAILQSTERKVDGLKKKGTFLIQLAAKTFPKPLHCLSL 337

Query: 181 RLTIDYYLLPPEQRNFPRSE----NLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSR 240
           +L  DY++L   + +  + +     LE    +HY +FSDNVLA +VVVNSTV+NAK+  R
Sbjct: 338 QLAADYFILGFNEEDAVKEDVSQKKLEDPSLYHYAIFSDNVLATSVVVNSTVLNAKEPQR 397

Query: 241 HVFHLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYY 300
           HVFH+VTD LNFGAM+MWF +N P +ATIQVEN+++FKWLNSSYC VLRQL+SA ++EYY
Sbjct: 398 HVFHIVTDKLNFGAMKMWFRINAPADATIQVENINDFKWLNSSYCSVLRQLESARLKEYY 457

Query: 301 FKAGHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGL 360
           FKA H  + S+GA +LKYRNPKYLSMLNHLRFYLP++YPKL+KILFLDDDIVVQKDL  L
Sbjct: 458 FKANHPSSISAGADNLKYRNPKYLSMLNHLRFYLPEVYPKLEKILFLDDDIVVQKDLAPL 517

Query: 361 WQVDLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEK 420
           W++D++GKVNGAVETC  +FHRFDKYLNFSN  I+  FD  ACGWA+GMN+FDLKEW+++
Sbjct: 518 WEIDMQGKVNGAVETCKESFHRFDKYLNFSNPKISENFDAGACGWAFGMNMFDLKEWRKR 577

Query: 421 NLTGIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIEN 480
           N+TGIYH WQ+LNEDR LW LG+LPPGL+TFY LTY +++SWHVLGLGY+P++N + IEN
Sbjct: 578 NITGIYHYWQDLNEDRTLWKLGSLPPGLITFYNLTYAMDRSWHVLGLGYDPALNQTAIEN 637

Query: 481 AAVIHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 516
           AAV+HYNGN KPW+ +A  KY+PYW+KY++YD+PY+R+C
Sbjct: 638 AAVVHYNGNYKPWLGLAFAKYKPYWSKYVEYDNPYLRRC 676

BLAST of MC05g0307 vs. TAIR 10
Match: AT4G38270.2 (galacturonosyltransferase 3 )

HSP 1 Score: 640.2 bits (1650), Expect = 1.4e-183
Identity = 299/519 (57.61%), Postives = 400/519 (77.07%), Query Frame = 0

Query: 1   QMVREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENENKNQDST 60
           Q++R+ER   + +EL++Q+ +T  +++   +  S    +S+ GKYS W+++ E+ N D+ 
Sbjct: 154 QILRQERRGQRTLELIRQEKETDEQMQEAAIQKSMSFENSVIGKYSIWRRDYESPNADAI 213

Query: 61  IRLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKA 120
           ++LMRDQIIMA+ Y +++K K   +LY  L     + +RV+G+A  DA+L  SA  + KA
Sbjct: 214 LKLMRDQIIMAKAYANIAKSKNVTNLYVFLMQQCGENKRVIGKATSDADLPSSALDQAKA 273

Query: 121 MAAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSL 180
           M   LS A+D+L+DC  + KK RA+L + E KV  LK++ TFL QL  K  P  L CLSL
Sbjct: 274 MGHALSLAKDELYDCHELAKKFRAILQSTERKVDGLKKKGTFLIQLAAKTFPKPLHCLSL 333

Query: 181 RLTIDYYLLPPEQRNFPRSE----NLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSR 240
           +L  DY++L   + +  + +     LE    +HY +FSDNVLA +VVVNSTV+NAK+  R
Sbjct: 334 QLAADYFILGFNEEDAVKEDVSQKKLEDPSLYHYAIFSDNVLATSVVVNSTVLNAKEPQR 393

Query: 241 HVFHLVTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYY 300
           HVFH+VTD LNFGAM+MWF +N P +ATIQVEN+++FKWLNSSYC VLRQL+SA ++EYY
Sbjct: 394 HVFHIVTDKLNFGAMKMWFRINAPADATIQVENINDFKWLNSSYCSVLRQLESARLKEYY 453

Query: 301 FKAGHSITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGL 360
           FKA H  + S+GA +LKYRNPKYLSMLNHLRFYLP++YPKL+KILFLDDDIVVQKDL  L
Sbjct: 454 FKANHPSSISAGADNLKYRNPKYLSMLNHLRFYLPEVYPKLEKILFLDDDIVVQKDLAPL 513

Query: 361 WQVDLEGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEK 420
           W++D++GKVNGAVETC  +FHRFDKYLNFSN  I+  FD  ACGWA+GMN+FDLKEW+++
Sbjct: 514 WEIDMQGKVNGAVETCKESFHRFDKYLNFSNPKISENFDAGACGWAFGMNMFDLKEWRKR 573

Query: 421 NLTGIYHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIEN 480
           N+TGIYH WQ+LNEDR LW LG+LPPGL+TFY LTY +++SWHVLGLGY+P++N + IEN
Sbjct: 574 NITGIYHYWQDLNEDRTLWKLGSLPPGLITFYNLTYAMDRSWHVLGLGYDPALNQTAIEN 633

Query: 481 AAVIHYNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 516
           AAV+HYNGN KPW+ +A  KY+PYW+KY++YD+PY+R+C
Sbjct: 634 AAVVHYNGNYKPWLGLAFAKYKPYWSKYVEYDNPYLRRC 672

BLAST of MC05g0307 vs. TAIR 10
Match: AT5G47780.1 (galacturonosyltransferase 4 )

HSP 1 Score: 588.2 bits (1515), Expect = 6.5e-168
Identity = 279/508 (54.92%), Postives = 372/508 (73.23%), Query Frame = 0

Query: 9   ENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKENEN-KNQDSTIRLMRDQ 68
           EN  ++L QQ  + + +    N   +              KK+  N    D+ +R ++DQ
Sbjct: 123 ENMHVQLTQQTSEKVDEQPEPNAFGA--------------KKDTGNVLMPDAQVRHLKDQ 182

Query: 69  IIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKAMAAILSE 128
           +I A++YLSL   K       EL++ +K+++R L +A+ D++L  +A +K+KAM   L++
Sbjct: 183 LIRAKVYLSLPSAKANAHFVRELRLRIKEVQRALADASKDSDLPKTAIEKLKAMEQTLAK 242

Query: 129 ARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSLRLTIDYY 188
            +    DC  V KKLRAML + +E++R  K+Q  FLTQL  K IP  L CL LRLT DYY
Sbjct: 243 GKQIQDDCSTVVKKLRAMLHSADEQLRVHKKQTMFLTQLTAKTIPKGLHCLPLRLTTDYY 302

Query: 189 LLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFHLVTDNLN 248
            L   ++ FP  E LE  + +HY LFSDNVLA +VVVNST+ NAK   +HVFH+VTD LN
Sbjct: 303 ALNSSEQQFPNQEKLEDTQLYHYALFSDNVLATSVVVNSTITNAKHPLKHVFHIVTDRLN 362

Query: 249 FGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAGHSITRSS 308
           + AMRMWFL NPP +ATIQV+N++EF WLNSSY PVL+QL S +M +YYF+A H    ++
Sbjct: 363 YAAMRMWFLDNPPGKATIQVQNVEEFTWLNSSYSPVLKQLSSRSMIDYYFRAHH----TN 422

Query: 309 GASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVDLEGKVNG 368
             ++LK+RNPKYLS+LNHLRFYLP+I+PKL K+LFLDDDIVVQKDL+GLW VDL+G VNG
Sbjct: 423 SDTNLKFRNPKYLSILNHLRFYLPEIFPKLSKVLFLDDDIVVQKDLSGLWSVDLKGNVNG 482

Query: 369 AVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTGIYHKWQN 428
           AVETC  +FHRFD+YLNFSN  I++ FDP ACGWAYGMN+FDL EWK +N+T +YH+WQ+
Sbjct: 483 AVETCGESFHRFDRYLNFSNPLISKNFDPRACGWAYGMNVFDLDEWKRQNITEVYHRWQD 542

Query: 429 LNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVIHYNGNMK 488
           LN+DR LW LGTLPPGL+TF+  TYPL++ WH+LGLGYNPS+N  +IE AAVIHYNGN+K
Sbjct: 543 LNQDRELWKLGTLPPGLITFWRRTYPLDRKWHILGLGYNPSVNQRDIERAAVIHYNGNLK 602

Query: 489 PWMEMAMTKYRPYWTKYMDYDHPYIRQC 516
           PW+E+ + +YR +W+K++DY+H Y+R+C
Sbjct: 603 PWLEIGIPRYRGFWSKHVDYEHVYLREC 612

BLAST of MC05g0307 vs. TAIR 10
Match: AT2G46480.1 (galacturonosyltransferase 2 )

HSP 1 Score: 575.5 bits (1482), Expect = 4.3e-164
Identity = 285/514 (55.45%), Postives = 365/514 (71.01%), Query Frame = 0

Query: 3   VREERYENQFMELVQQDDDTIVKLENENMLASELVGSSIHGKYSTWKKE-NENKNQDSTI 62
           +R+E+   +  EL+  +DD++ KLE   M  S  V S+  G Y+ WK E    K+ +  +
Sbjct: 40  LRQEKRLERANELM--NDDSLQKLETAAMARSRSVDSAPLGNYTIWKNEYRRGKSFEDML 99

Query: 63  RLMRDQIIMARIYLSLSKKKKKFDLYEELQIHLKKIRRVLGEANIDAELHYSAPKKIKAM 122
           RLM+DQIIMAR+Y  L+K      L++E++  L K+     E + D +        I+ M
Sbjct: 100 RLMQDQIIMARVYSGLAKFTNNLALHQEIETQLMKL--AWEEESTDIDQEQRVLDSIRDM 159

Query: 123 AAILSEARDQLFDCKLVTKKLRAMLLTEEEKVRRLKRQNTFLTQLGVKGIPGALRCLSLR 182
             IL+ A +QL++CKLVT KLRAML T E+++   +   TFLTQL  K +P A+ CL++R
Sbjct: 160 GQILARAHEQLYECKLVTNKLRAMLQTVEDELENEQTYITFLTQLASKALPDAIHCLTMR 219

Query: 183 LTIDYYLLPPEQRNFPRSENLESKKFHHYGLFSDNVLAAAVVVNSTVMNAKDSSRHVFHL 242
           L ++Y+LLP   RNFPR ENLE+ K +HY LFSDNVLAA+VVVNSTVMNA+D SRHVFHL
Sbjct: 220 LNLEYHLLPLPMRNFPRRENLENPKLYHYALFSDNVLAASVVVNSTVMNAQDPSRHVFHL 279

Query: 243 VTDNLNFGAMRMWFLLNPPKEATIQVENLDEFKWLNSSYCPVLRQLQSAAMREYYFKAGH 302
           VTD LNFGAM MWFLLNPP EATI V+  ++F WLNSSY PVL QL+SAAM+++YFK   
Sbjct: 280 VTDKLNFGAMSMWFLLNPPGEATIHVQRFEDFTWLNSSYSPVLSQLESAAMKKFYFKTAR 339

Query: 303 SITRSSGASSLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWQVDL 362
           S +  SG+ +LKYR PKY+SMLNHLRFY+P+I+PKL+KILF+DDD+VVQKDLT LW +DL
Sbjct: 340 SESVESGSENLKYRYPKYMSMLNHLRFYIPRIFPKLEKILFVDDDVVVQKDLTPLWSIDL 399

Query: 363 EGKVNGAVETCVLNFHRFDKYLNFSNSYIAREFDPNACGWAYGMNIFDLKEWKEKNLTGI 422
           +GKVN                           FDP  CGWAYGMNIFDLKEWK+ N+T  
Sbjct: 400 KGKVN-------------------------ENFDPKFCGWAYGMNIFDLKEWKKNNITET 459

Query: 423 YHKWQNLNEDRLLWSLGTLPPGLMTFYGLTYPLEKSWHVLGLGYNPSINLSEIENAAVIH 482
           YH WQNLNE+R LW LGTLPPGL+TFY LT PL++ WH+LGLGY+  I++ +IE +AVIH
Sbjct: 460 YHFWQNLNENRTLWKLGTLPPGLITFYNLTQPLQRKWHLLGLGYDKGIDVKKIERSAVIH 519

Query: 483 YNGNMKPWMEMAMTKYRPYWTKYMDYDHPYIRQC 516
           YNG+MKPW EM ++KY+PYWTKY ++DHPYI  C
Sbjct: 520 YNGHMKPWTEMGISKYQPYWTKYTNFDHPYIFTC 524