MC05g0305 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0305
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionglyoxylate/hydroxypyruvate reductase HPR3-like
LocationMC05: 2330176 .. 2331438 (+)
RNA-Seq ExpressionMC05g0305
SyntenyMC05g0305
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCATGGCGACCAAAATCCATGGCGACGAGCTTCCTCAAGTTTTGGTTCTAGGCTCCCCCTGGGTATTACCCTCTCTCGAATCCCAATTCTCACACAGATTCCATTTCCTCAAACCATCCAATTCCCAATTCCCACTACTCCAATTCCTCTCCTCTTACGCCCAATCCGTCCAAGCCTTGCTCATCCCAGGCGGCAGTTTCGCCCTAACCTCCGCCATTCTCGACTGCCTCCCGTCGCTGAAGCTCGCCGTCACCACCAGCGTCGGCGTCGACCACTTGGACTTGCCGGAGTTGCGCCGCCGTGGGATCGCCGTTGCCTATGCCGGAAACTTGTACTCTGAAGATGTCGCCGATATGGCGGTTGGATTGCTAATCGACGTTCTTAGGAAAGTGTCGGCCGCGGACCGGTTCCTGAGGCAAGGGCTTTGGCCTACCAATCGGGAGTTTCCTCTTGGATTGAAGGTGCGATGCTCTTTACTTTCCATTTCTAAACGGAAAGTAATTTATAAACACATTGGTCCCTCGTTGGTTTATTTATCTAAATGTATAGGAAATTTAACAATTTGTTTACGATAGAAACTAAATCGTTACAAATTAAATACTACTCAAACTAAATTATTACATAACTATACTTTGAGAACCAAATTTTTTACAAATTTAAAAGTATTGGGACTAAATCGTTACAAGGTAAGGTTTAGAAACTAAATCGTTACTTGAAATTTTTTTAACATTTGGAAATTTTCACAGTTGAGCGGGAAACGAATTGGGATTGTTGGACTGGGGAAAATAGGGTGTGAAGTAGCCAAAAGGCTGGAGGGATTTGGGTGCAAGATGTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATACTCATACCATTCCACTGTACATGAACTGGCAGCAGAGTGTGAAGCTCTGATCGTCTGTTGTGGGTTAACTGAAGAAACCCGCCATATGATCGACAGGGAGGTGATGGTTGCATTGGGAAAAGATGGTGTGATAATCAACGTTGGTCGCGGGGCGATCATCGATGAGAAGGAGCTGATTGAATGTCTGTTACAAGGAGAGATTGGGGGAGCTGGATTGGATGTGTTTGAGAATGAACCTGAAATTCCAGAAGAGCTGTTTGGGGTTGATAATGTTGTGTTGTCGCCACATGTTGCTGTGATTACACATGAATCTATGAAGGGATTGTGTAAGTTGGTGGTGGACAACTTGGAAGCTTTCTTCTCAAATAAACCTCTTGTGTCTCCCTTTATG

mRNA sequence

TCCATGGCGACCAAAATCCATGGCGACGAGCTTCCTCAAGTTTTGGTTCTAGGCTCCCCCTGGGTATTACCCTCTCTCGAATCCCAATTCTCACACAGATTCCATTTCCTCAAACCATCCAATTCCCAATTCCCACTACTCCAATTCCTCTCCTCTTACGCCCAATCCGTCCAAGCCTTGCTCATCCCAGGCGGCAGTTTCGCCCTAACCTCCGCCATTCTCGACTGCCTCCCGTCGCTGAAGCTCGCCGTCACCACCAGCGTCGGCGTCGACCACTTGGACTTGCCGGAGTTGCGCCGCCGTGGGATCGCCGTTGCCTATGCCGGAAACTTGTACTCTGAAGATGTCGCCGATATGGCGGTTGGATTGCTAATCGACGTTCTTAGGAAAGTGTCGGCCGCGGACCGGTTCCTGAGGCAAGGGCTTTGGCCTACCAATCGGGAGTTTCCTCTTGGATTGAAGTTGAGCGGGAAACGAATTGGGATTGTTGGACTGGGGAAAATAGGGTGTGAAGTAGCCAAAAGGCTGGAGGGATTTGGGTGCAAGATGTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATACTCATACCATTCCACTGTACATGAACTGGCAGCAGAGTGTGAAGCTCTGATCGTCTGTTGTGGGTTAACTGAAGAAACCCGCCATATGATCGACAGGGAGGTGATGGTTGCATTGGGAAAAGATGGTGTGATAATCAACGTTGGTCGCGGGGCGATCATCGATGAGAAGGAGCTGATTGAATGTCTGTTACAAGGAGAGATTGGGGGAGCTGGATTGGATGTGTTTGAGAATGAACCTGAAATTCCAGAAGAGCTGTTTGGGGTTGATAATGTTGTGTTGTCGCCACATGTTGCTGTGATTACACATGAATCTATGAAGGGATTGTGTAAGTTGGTGGTGGACAACTTGGAAGCTTTCTTCTCAAATAAACCTCTTGTGTCTCCCTTTATG

Coding sequence (CDS)

TCCATGGCGACCAAAATCCATGGCGACGAGCTTCCTCAAGTTTTGGTTCTAGGCTCCCCCTGGGTATTACCCTCTCTCGAATCCCAATTCTCACACAGATTCCATTTCCTCAAACCATCCAATTCCCAATTCCCACTACTCCAATTCCTCTCCTCTTACGCCCAATCCGTCCAAGCCTTGCTCATCCCAGGCGGCAGTTTCGCCCTAACCTCCGCCATTCTCGACTGCCTCCCGTCGCTGAAGCTCGCCGTCACCACCAGCGTCGGCGTCGACCACTTGGACTTGCCGGAGTTGCGCCGCCGTGGGATCGCCGTTGCCTATGCCGGAAACTTGTACTCTGAAGATGTCGCCGATATGGCGGTTGGATTGCTAATCGACGTTCTTAGGAAAGTGTCGGCCGCGGACCGGTTCCTGAGGCAAGGGCTTTGGCCTACCAATCGGGAGTTTCCTCTTGGATTGAAGTTGAGCGGGAAACGAATTGGGATTGTTGGACTGGGGAAAATAGGGTGTGAAGTAGCCAAAAGGCTGGAGGGATTTGGGTGCAAGATGTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATACTCATACCATTCCACTGTACATGAACTGGCAGCAGAGTGTGAAGCTCTGATCGTCTGTTGTGGGTTAACTGAAGAAACCCGCCATATGATCGACAGGGAGGTGATGGTTGCATTGGGAAAAGATGGTGTGATAATCAACGTTGGTCGCGGGGCGATCATCGATGAGAAGGAGCTGATTGAATGTCTGTTACAAGGAGAGATTGGGGGAGCTGGATTGGATGTGTTTGAGAATGAACCTGAAATTCCAGAAGAGCTGTTTGGGGTTGATAATGTTGTGTTGTCGCCACATGTTGCTGTGATTACACATGAATCTATGAAGGGATTGTGTAAGTTGGTGGTGGACAACTTGGAAGCTTTCTTCTCAAATAAACCTCTTGTGTCTCCCTTTATG

Protein sequence

SMATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALLIPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAVGLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGCKMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVIINVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESMKGLCKLVVDNLEAFFSNKPLVSPFM
Homology
BLAST of MC05g0305 vs. ExPASy Swiss-Prot
Match: Q9LE33 (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR3 PE=2 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.3e-90
Identity = 167/314 (53.18%), Postives = 224/314 (71.34%), Query Frame = 0

Query: 12  PQVLVLGSPWVLPSLESQFSHRFH-FLKPSNSQFPLLQFLSSYAQSVQALLIPGGSFALT 71
           P V++L  P  L  ++   +  F   +  ++S   L  F   +A S +A +I  G   +T
Sbjct: 7   PPVVLLHRPPSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVI-SGRLPVT 66

Query: 72  SAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAVGLLIDVLRK 131
             +L  LPSL++ V TSVG+DH+DL   +RRGI +  AGN +S+DVAD AVGLLI VLR+
Sbjct: 67  DELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRR 126

Query: 132 VSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGCKMSYNSRTK 191
           + AADR++R G W    +F LG K+SGKR+GIVGLG IG  VAKRLE FGC +SYNSR++
Sbjct: 127 IPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQ 186

Query: 192 KPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVIINVGRGAIID 251
           K   PY Y+S +  LA   + L++CC LT+ET H+++REVM  LGKDGV+INVGRG +ID
Sbjct: 187 KQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLID 246

Query: 252 EKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESMKGLCKLVVD 311
           EKE+++CL+ G IGGAGLDVFENEP +P+ELFG+DNVVLSPH AV T  S+  + ++ + 
Sbjct: 247 EKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALA 306

Query: 312 NLEAFFSNKPLVSP 325
           NL+AFFSN+PL+SP
Sbjct: 307 NLKAFFSNRPLLSP 319

BLAST of MC05g0305 vs. ExPASy Swiss-Prot
Match: A5CAL1 (Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN=2KGR PE=1 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.7e-71
Identity = 140/303 (46.20%), Postives = 202/303 (66.67%), Query Frame = 0

Query: 24  PSLESQFSHRFHFLKPSNSQFPLLQFL-SSYAQSVQALLIPGGSFALTSA-ILDCLPSLK 83
           P LE +   RF   +     FP    L   ++ S++A++  G SF    A +++ LP ++
Sbjct: 14  PYLEQELDKRFKLFR--FWDFPSANDLFREHSNSIRAVV--GNSFIGADAQMIEALPKME 73

Query: 84  LAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAVGLLIDVLRKVSAADRFLRQG 143
           +  + SVG+D +DL   + +GI V    ++ +EDVAD+A+ L++  LR++  +DR++R G
Sbjct: 74  IVSSFSVGLDKIDLVRCKEKGIRVTNTPDVLTEDVADLALALILATLRRICESDRYVRSG 133

Query: 144 LWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGCKMSYNSRTKKPLVPYSYHST 203
            W    +F L  K +GK +GI+GLG+IG  +AKR EGF C +SY+SRT+KP   Y Y+ +
Sbjct: 134 SWKKG-DFKLTTKFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEKPGTNYKYYPS 193

Query: 204 VHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVIINVGRGAIIDEKELIECLLQG 263
           V ELA+ C+ L+V C LT ETRH+I+REV+ ALG  GV+IN+GRG  +DE EL+  L++G
Sbjct: 194 VVELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPELVSALVEG 253

Query: 264 EIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESMKGLCKLVVDNLEAFFSNKPL 323
            +GGAGLDVFENEP +PEEL  +DNVVL PHV   T E+ K +  LV+ NLEA F NKPL
Sbjct: 254 RLGGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGNLEAHFLNKPL 311

Query: 324 VSP 325
           ++P
Sbjct: 314 LTP 311

BLAST of MC05g0305 vs. ExPASy Swiss-Prot
Match: Q65CJ7 (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR PE=1 SV=2)

HSP 1 Score: 269.6 bits (688), Expect = 4.5e-71
Identity = 141/307 (45.93%), Postives = 209/307 (68.08%), Query Frame = 0

Query: 19  SPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALLIPGGSFALTSA-ILDCL 78
           S ++   L+ +F    ++ +P+   F  LQ     A+S++A++  G S A   A ++D L
Sbjct: 13  STYLEQELDKRFKLFRYWTQPAQRDFLALQ-----AESIRAVV--GNSNAGADAELIDAL 72

Query: 79  PSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAVGLLIDVLRKVSAADRF 138
           P L++  + SVG+D +DL +   +G+ V    ++ ++DVAD+A+GL++ VLR++   D++
Sbjct: 73  PKLEIVSSFSVGLDKVDLIKCEEKGVRVTNTPDVLTDDVADLAIGLILAVLRRICECDKY 132

Query: 139 LRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGCKMSYNSRTKKPLVPYS 198
           +R+G W    +F L  K SGKR+GI+GLG+IG  VA+R E F C +SY SR+KKP   Y+
Sbjct: 133 VRRGAWKFG-DFKLTTKFSGKRVGIIGLGRIGLAVAERAEAFDCPISYFSRSKKPNTNYT 192

Query: 199 YHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVIINVGRGAIIDEKELIEC 258
           Y+ +V ELA+  + L+V C LT ET H+I+REV+ ALG  GV+IN+GRG  +DE EL+  
Sbjct: 193 YYGSVVELASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDEPELVSA 252

Query: 259 LLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESMKGLCKLVVDNLEAFFS 318
           L++G +GGAGLDVFE EPE+PE+LFG++NVVL PHV   T E+ K +  LVV NLEA FS
Sbjct: 253 LVEGRLGGAGLDVFEREPEVPEKLFGLENVVLLPHVGSGTVETRKVMADLVVGNLEAHFS 311

Query: 319 NKPLVSP 325
            KPL++P
Sbjct: 313 GKPLLTP 311

BLAST of MC05g0305 vs. ExPASy Swiss-Prot
Match: Q9CA90 (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=HPR2 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 8.6e-70
Identity = 137/299 (45.82%), Postives = 200/299 (66.89%), Query Frame = 0

Query: 26  LESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALLIPGGSFALTSAILDCLPSLKLAVT 85
           LE++   RF+ L+   S    +  L ++  S++A ++   S    + ++  LP+L++  +
Sbjct: 16  LENELEKRFNLLRFWTSPEKSV-LLETHRNSIRA-VVGNASAGADAQLISDLPNLEIVSS 75

Query: 86  TSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAVGLLIDVLRKVSAADRFLRQGLWPT 145
            SVG+D +DL + + +GI V    ++ +EDVAD+A+GL++ +LR++   DR++R G W  
Sbjct: 76  FSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDRYVRSGKWKQ 135

Query: 146 NREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGCKMSYNSRTKKPLVPYSYHSTVHEL 205
             EF L  K SGK +GI+GLG+IG  +AKR E F C ++Y SRT KP V Y Y+ TV +L
Sbjct: 136 G-EFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPDVAYKYYPTVVDL 195

Query: 206 AAECEALIVCCGLTEETRHMIDREVMVALGKDGVIINVGRGAIIDEKELIECLLQGEIGG 265
           A   + L+V C LTE+TRH++DR+VM ALG  GV+IN+GRG  +DE+ELI+ L +G +GG
Sbjct: 196 AQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGRLGG 255

Query: 266 AGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESMKGLCKLVVDNLEAFFSNKPLVSP 325
           A LDVFE EP +PEELFG++NVVL PHV   T E+   +  LVV NLEA FS K L++P
Sbjct: 256 AALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLEAHFSGKSLLTP 311

BLAST of MC05g0305 vs. ExPASy Swiss-Prot
Match: Q5FTU6 (2-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GOX0417 PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.8e-52
Identity = 110/278 (39.57%), Postives = 165/278 (59.35%), Query Frame = 0

Query: 47  LQFLSSYAQSVQALLIPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVA 106
           L+ L + A +++ +   GGS  + S I+D LP+L++     VG D ++L E RRR I VA
Sbjct: 33  LENLKNIAPAIRGITTGGGS-GVPSEIMDALPNLEVISVNGVGTDRINLDEARRRNIGVA 92

Query: 107 YAGNLYSEDVADMAVGLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLG 166
              N  ++DVADMAV L++ V+R +   D F+R G WP +   PLG  L+ K++GI G G
Sbjct: 93  ITQNTLTDDVADMAVALMMAVMRSIVTNDAFVRAGKWP-SATAPLGRSLTRKKVGIAGFG 152

Query: 167 KIGCEVAKRLEGFGCKMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMI 226
            IG  +AKR+  FG +++Y +   +P     +   +  LA  C+ LI+       + +MI
Sbjct: 153 HIGQAIAKRVSAFGMEVAYFNSHARPESTCHFEPDLKALATWCDVLILAVSGGPRSANMI 212

Query: 227 DREVMVALGKDGVIINVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDN 286
           DR+ + ALGKDG ++N+ RG ++DE  L+  L +  I GAGLDVF+NEP I      + N
Sbjct: 213 DRDTLDALGKDGFLVNIARGTVVDEAALLSALQEKRIAGAGLDVFQNEPNINPAFLSLPN 272

Query: 287 VVLSPHVAVITHESMKGLCKLVVDNLEAFFSNKPLVSP 325
            VL  H A  T E+   +  LVVDNL A+F++K L++P
Sbjct: 273 TVLQAHQASATVETRTTMANLVVDNLIAYFTDKTLLTP 308

BLAST of MC05g0305 vs. NCBI nr
Match: XP_022159358.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia])

HSP 1 Score: 648 bits (1671), Expect = 2.20e-234
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 2   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 61
           MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL
Sbjct: 1   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 60

Query: 62  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 121
           IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV
Sbjct: 61  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 120

Query: 122 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 181
           GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC
Sbjct: 121 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 180

Query: 182 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 241
           KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII
Sbjct: 181 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 240

Query: 242 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 301
           NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM
Sbjct: 241 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 300

Query: 302 KGLCKLVVDNLEAFFSNKPLVSPFM 326
           KGLCKLVVDNLEAFFSNKPLVSPFM
Sbjct: 301 KGLCKLVVDNLEAFFSNKPLVSPFM 325

BLAST of MC05g0305 vs. NCBI nr
Match: KAG6597176.1 (Glyoxylate/hydroxypyruvate reductase HPR3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 535 bits (1378), Expect = 1.01e-189
Identity = 259/324 (79.94%), Postives = 295/324 (91.05%), Query Frame = 0

Query: 2   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 61
           MA+ +H  ELP+VLVLG PWVLPSLESQFS+RFHFLKPSNS  PLLQFLSS+AQS +ALL
Sbjct: 1   MASGVHSGELPEVLVLGFPWVLPSLESQFSNRFHFLKPSNSDLPLLQFLSSFAQSARALL 60

Query: 62  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 121
           IPGG   LT+AILDCLP+LKL VTTS G+DHLDLPELRRR IA+AYAGNL+++DVAD+AV
Sbjct: 61  IPGGGIQLTTAILDCLPALKLVVTTSTGLDHLDLPELRRREIAIAYAGNLFTQDVADLAV 120

Query: 122 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 181
           GLLIDVL KVSA DRF++QG+WP+ R+FP GLKLSGK+IGIVGLGKIG EVAKRLEGFGC
Sbjct: 121 GLLIDVLMKVSAGDRFVKQGIWPSKRKFPPGLKLSGKQIGIVGLGKIGTEVAKRLEGFGC 180

Query: 182 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 241
           ++SYNSRTKKPLVPYSY+S VHELAA CEALI+CCGLT ETRHMIDR+VMVALGKDGVI+
Sbjct: 181 RISYNSRTKKPLVPYSYYSNVHELAANCEALIICCGLTAETRHMIDRDVMVALGKDGVIV 240

Query: 242 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 301
           NVGRGAI+DE EL+  L+QGE+GGAGLDVFENEPE+PEELF +DNVVLSPHVAV+T ESM
Sbjct: 241 NVGRGAIVDETELVRRLIQGELGGAGLDVFENEPEVPEELFNLDNVVLSPHVAVLTTESM 300

Query: 302 KGLCKLVVDNLEAFFSNKPLVSPF 325
            GL +LV +NLEAFFSNKPLV+PF
Sbjct: 301 VGLTQLVTENLEAFFSNKPLVTPF 324

BLAST of MC05g0305 vs. NCBI nr
Match: XP_022950894.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita moschata])

HSP 1 Score: 532 bits (1371), Expect = 1.18e-188
Identity = 260/324 (80.25%), Postives = 294/324 (90.74%), Query Frame = 0

Query: 2   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 61
           MA+  H  ELP+VLVLG PWVLPSLESQFS+RFHFLKPSNS  PLLQFLSS+AQS +ALL
Sbjct: 1   MASGGHSGELPEVLVLGFPWVLPSLESQFSNRFHFLKPSNSDLPLLQFLSSFAQSARALL 60

Query: 62  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 121
           IPGG   LTSAILDCLP+LKL VTTS G+DHLDLPELRRR I +AYAGNL+++DVAD+AV
Sbjct: 61  IPGGGIQLTSAILDCLPALKLVVTTSTGLDHLDLPELRRREIDIAYAGNLFTQDVADLAV 120

Query: 122 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 181
           GLLIDVL KVSA DRF++QG+WP+ R+F LGLKLSGK+IGIVGLGKIG EVAKRLEGFGC
Sbjct: 121 GLLIDVLMKVSAGDRFVKQGIWPSKRKFSLGLKLSGKQIGIVGLGKIGTEVAKRLEGFGC 180

Query: 182 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 241
           ++SYNSRTKKPLVPYSY+S VHELAA CEALI+CCGLT ETRHMIDR+VMVALGKDGVI+
Sbjct: 181 RISYNSRTKKPLVPYSYYSNVHELAANCEALIICCGLTAETRHMIDRDVMVALGKDGVIV 240

Query: 242 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 301
           NVGRGAI+DE EL+  L+QGE+GGAGLDVFENEPE+PEELF +DNVVLSPHVAV+T ESM
Sbjct: 241 NVGRGAIVDETELVRRLIQGELGGAGLDVFENEPEVPEELFNLDNVVLSPHVAVLTTESM 300

Query: 302 KGLCKLVVDNLEAFFSNKPLVSPF 325
            GL +LVV+NLEAFFSNKPLV+PF
Sbjct: 301 VGLTQLVVENLEAFFSNKPLVTPF 324

BLAST of MC05g0305 vs. NCBI nr
Match: XP_023539654.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 531 bits (1369), Expect = 2.38e-188
Identity = 260/324 (80.25%), Postives = 294/324 (90.74%), Query Frame = 0

Query: 2   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 61
           MA+  H  ELP+VLVLG PWVLPSLESQFS+RFHFLKPSNS  PLLQFLSS+AQS +ALL
Sbjct: 1   MASGGHSGELPEVLVLGFPWVLPSLESQFSNRFHFLKPSNSDLPLLQFLSSFAQSARALL 60

Query: 62  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 121
           IPGG   LTSAILDCLP+LKL VTTS G+DHLDLPELRRR IA+AYAGNL+S+DVAD+AV
Sbjct: 61  IPGGGIQLTSAILDCLPALKLVVTTSTGLDHLDLPELRRREIAIAYAGNLFSQDVADLAV 120

Query: 122 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 181
           GLLIDVL KVSA DRF++QG+WP+ R+F LGLKLSGK+IGIVGLGKIG EVAKRLEGFGC
Sbjct: 121 GLLIDVLMKVSAGDRFVKQGIWPSQRKFSLGLKLSGKQIGIVGLGKIGTEVAKRLEGFGC 180

Query: 182 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 241
           ++SYNSRTKKPLVPYSY+S VHELAA CEALI+CCGLT ETRHMIDR+VMVALGKDGVI+
Sbjct: 181 RISYNSRTKKPLVPYSYYSNVHELAANCEALIICCGLTAETRHMIDRDVMVALGKDGVIV 240

Query: 242 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 301
           NVGRGAI+DE EL+  L++GE+GGAGLDVFENEPE+P ELF +DNVVLSPHVAV+T ESM
Sbjct: 241 NVGRGAIVDEMELVRRLIRGELGGAGLDVFENEPEVPMELFNLDNVVLSPHVAVLTTESM 300

Query: 302 KGLCKLVVDNLEAFFSNKPLVSPF 325
            GL +LVV+NLEAFFSNKPLV+PF
Sbjct: 301 VGLTQLVVENLEAFFSNKPLVTPF 324

BLAST of MC05g0305 vs. NCBI nr
Match: XP_022147257.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia])

HSP 1 Score: 530 bits (1366), Expect = 7.89e-188
Identity = 265/323 (82.04%), Postives = 288/323 (89.16%), Query Frame = 0

Query: 2   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 61
           MA +    +LPQVLVLG P V   LESQF +RFHFLKP  S+ PL QFL+SYAQ VQAL+
Sbjct: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60

Query: 62  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 121
           IPGGS ALTSAILDCLPSLKL  T S GVDHLDLPELRRRG+AVAYAGNL+SEDVADMAV
Sbjct: 61  IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV 120

Query: 122 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 181
           GLLIDVLRKVSA DRF+RQGLW T   FPLGLKLSGKRIGIVGLG+IGCEVAKRLEGFGC
Sbjct: 121 GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC 180

Query: 182 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 241
           ++SYNSRTKKPLVPYSY+S VHELAAECEALI+CCGLTEETRHMI+REVMVALGKDGVII
Sbjct: 181 RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII 240

Query: 242 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 301
           N+GRGAI+DEKE+I CLLQGEI GAGLDVFENEPEIPE+LF +DNVVLSPHVAV THE+ 
Sbjct: 241 NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF 300

Query: 302 KGLCKLVVDNLEAFFSNKPLVSP 324
             L KL+VDNLEAFFSN+PLVSP
Sbjct: 301 LRLSKLMVDNLEAFFSNRPLVSP 323

BLAST of MC05g0305 vs. ExPASy TrEMBL
Match: A0A6J1DYK8 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN=LOC111025775 PE=3 SV=1)

HSP 1 Score: 648 bits (1671), Expect = 1.07e-234
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 2   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 61
           MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL
Sbjct: 1   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 60

Query: 62  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 121
           IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV
Sbjct: 61  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 120

Query: 122 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 181
           GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC
Sbjct: 121 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 180

Query: 182 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 241
           KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII
Sbjct: 181 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 240

Query: 242 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 301
           NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM
Sbjct: 241 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 300

Query: 302 KGLCKLVVDNLEAFFSNKPLVSPFM 326
           KGLCKLVVDNLEAFFSNKPLVSPFM
Sbjct: 301 KGLCKLVVDNLEAFFSNKPLVSPFM 325

BLAST of MC05g0305 vs. ExPASy TrEMBL
Match: A0A6J1GH55 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita moschata OX=3662 GN=LOC111453830 PE=3 SV=1)

HSP 1 Score: 532 bits (1371), Expect = 5.72e-189
Identity = 260/324 (80.25%), Postives = 294/324 (90.74%), Query Frame = 0

Query: 2   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 61
           MA+  H  ELP+VLVLG PWVLPSLESQFS+RFHFLKPSNS  PLLQFLSS+AQS +ALL
Sbjct: 1   MASGGHSGELPEVLVLGFPWVLPSLESQFSNRFHFLKPSNSDLPLLQFLSSFAQSARALL 60

Query: 62  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 121
           IPGG   LTSAILDCLP+LKL VTTS G+DHLDLPELRRR I +AYAGNL+++DVAD+AV
Sbjct: 61  IPGGGIQLTSAILDCLPALKLVVTTSTGLDHLDLPELRRREIDIAYAGNLFTQDVADLAV 120

Query: 122 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 181
           GLLIDVL KVSA DRF++QG+WP+ R+F LGLKLSGK+IGIVGLGKIG EVAKRLEGFGC
Sbjct: 121 GLLIDVLMKVSAGDRFVKQGIWPSKRKFSLGLKLSGKQIGIVGLGKIGTEVAKRLEGFGC 180

Query: 182 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 241
           ++SYNSRTKKPLVPYSY+S VHELAA CEALI+CCGLT ETRHMIDR+VMVALGKDGVI+
Sbjct: 181 RISYNSRTKKPLVPYSYYSNVHELAANCEALIICCGLTAETRHMIDRDVMVALGKDGVIV 240

Query: 242 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 301
           NVGRGAI+DE EL+  L+QGE+GGAGLDVFENEPE+PEELF +DNVVLSPHVAV+T ESM
Sbjct: 241 NVGRGAIVDETELVRRLIQGELGGAGLDVFENEPEVPEELFNLDNVVLSPHVAVLTTESM 300

Query: 302 KGLCKLVVDNLEAFFSNKPLVSPF 325
            GL +LVV+NLEAFFSNKPLV+PF
Sbjct: 301 VGLTQLVVENLEAFFSNKPLVTPF 324

BLAST of MC05g0305 vs. ExPASy TrEMBL
Match: A0A6J1D1V9 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN=LOC111016253 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 3.82e-188
Identity = 265/323 (82.04%), Postives = 288/323 (89.16%), Query Frame = 0

Query: 2   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 61
           MA +    +LPQVLVLG P V   LESQF +RFHFLKP  S+ PL QFL+SYAQ VQAL+
Sbjct: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60

Query: 62  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 121
           IPGGS ALTSAILDCLPSLKL  T S GVDHLDLPELRRRG+AVAYAGNL+SEDVADMAV
Sbjct: 61  IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV 120

Query: 122 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 181
           GLLIDVLRKVSA DRF+RQGLW T   FPLGLKLSGKRIGIVGLG+IGCEVAKRLEGFGC
Sbjct: 121 GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC 180

Query: 182 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 241
           ++SYNSRTKKPLVPYSY+S VHELAAECEALI+CCGLTEETRHMI+REVMVALGKDGVII
Sbjct: 181 RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII 240

Query: 242 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 301
           N+GRGAI+DEKE+I CLLQGEI GAGLDVFENEPEIPE+LF +DNVVLSPHVAV THE+ 
Sbjct: 241 NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF 300

Query: 302 KGLCKLVVDNLEAFFSNKPLVSP 324
             L KL+VDNLEAFFSN+PLVSP
Sbjct: 301 LRLSKLMVDNLEAFFSNRPLVSP 323

BLAST of MC05g0305 vs. ExPASy TrEMBL
Match: A0A6J1I9J9 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita maxima OX=3661 GN=LOC111472757 PE=3 SV=1)

HSP 1 Score: 521 bits (1341), Expect = 2.12e-184
Identity = 253/324 (78.09%), Postives = 292/324 (90.12%), Query Frame = 0

Query: 2   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 61
           MA+  H  +LP+VLVLG PWVLPSLESQFS+RF FLKPSNS  PLLQFLSS+AQS +ALL
Sbjct: 1   MASGGHSGDLPEVLVLGFPWVLPSLESQFSNRFQFLKPSNSDLPLLQFLSSFAQSARALL 60

Query: 62  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 121
           IPGG   LTSAILDCLP+LKL VTTS G+DHLDLPELRRR IA+AYAGNL+++DVAD+AV
Sbjct: 61  IPGGGIQLTSAILDCLPALKLVVTTSSGLDHLDLPELRRREIAIAYAGNLFTQDVADLAV 120

Query: 122 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 181
           GLLIDVLRKVSA DRF++QG+WP+ R+F LGLKLSGK+IGIVGLGKIG EVAKRLEGFGC
Sbjct: 121 GLLIDVLRKVSAGDRFVKQGIWPSKRKFSLGLKLSGKQIGIVGLGKIGTEVAKRLEGFGC 180

Query: 182 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 241
           K+SYNSRTKKP +PY ++S +HELAA CEALI+CCGLT ETRHMIDR+VMVALG+DGVI+
Sbjct: 181 KISYNSRTKKPTLPYPFYSDLHELAANCEALIICCGLTAETRHMIDRDVMVALGRDGVIV 240

Query: 242 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 301
           NVGRGAI+DE EL+  L+QGE+GGAGLDVFENEPE+P+ELF +DNVVLSPHVAV+T ESM
Sbjct: 241 NVGRGAIVDETELVRRLIQGELGGAGLDVFENEPEVPKELFNLDNVVLSPHVAVLTTESM 300

Query: 302 KGLCKLVVDNLEAFFSNKPLVSPF 325
            GL +LV +NLEAFFSNKPLV+PF
Sbjct: 301 VGLTQLVAENLEAFFSNKPLVTPF 324

BLAST of MC05g0305 vs. ExPASy TrEMBL
Match: A0A6J1D073 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN=LOC111016079 PE=3 SV=1)

HSP 1 Score: 503 bits (1295), Expect = 1.98e-177
Identity = 252/326 (77.30%), Postives = 284/326 (87.12%), Query Frame = 0

Query: 2   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 61
           MA +I  DELPQVLVL  P    SLESQF +RFHFLKP  S+ PLLQFL+SYAQS  ALL
Sbjct: 1   MAVEIPADELPQVLVLAPPADFSSLESQFPNRFHFLKPWFSKLPLLQFLTSYAQSAPALL 60

Query: 62  I-PGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMA 121
           + PG   A++S ILDCLPSLKL VT S GVDHLDLPELRR G+A+AYAGNL+SEDVADMA
Sbjct: 61  VSPGDRLAVSSNILDCLPSLKLVVTISAGVDHLDLPELRRCGVAIAYAGNLFSEDVADMA 120

Query: 122 VGLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFG 181
           VGLLIDVLRKVSA DRF+R+GLWPT    PLGLKLSGKRIGIVGLGKIG EVAKRLEGFG
Sbjct: 121 VGLLIDVLRKVSAGDRFVRKGLWPTKGNPPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 182 CKMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVI 241
           CK+SYNSRTKKP+V YSY+S V+ELA  CEA+I+CCGLT+ETRHMI+REVM+ALGKDGV+
Sbjct: 181 CKLSYNSRTKKPIVSYSYYSNVYELATNCEAIIICCGLTKETRHMINREVMLALGKDGVM 240

Query: 242 INVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHES 301
           +NVGRGAIIDEKE+I+CL++GEIGG GLDVFENEPEIPE+LF +DNVVLSPHVAV T ES
Sbjct: 241 VNVGRGAIIDEKEMIQCLIEGEIGGVGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTKES 300

Query: 302 MKGLCKLVVDNLEAFFSNKPLVSPFM 326
           M+GL KL  DNLEA F+NKPLVSPF+
Sbjct: 301 MEGLVKLATDNLEALFANKPLVSPFV 326

BLAST of MC05g0305 vs. TAIR 10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 352.8 bits (904), Expect = 2.9e-97
Identity = 178/329 (54.10%), Postives = 245/329 (74.47%), Query Frame = 0

Query: 1   SMATKIHGDELPQVLVLGSPWVLPSLESQF--SHRFHFLKPSNSQFPLLQFLSSYAQSVQ 60
           +MAT    ++LP+VL++  P  +  L   F  S +F  LK   S  PL +FL+ ++ S+ 
Sbjct: 11  TMATSSSTEKLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSIS 70

Query: 61  ALLIPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVAD 120
           A++ P  +  +T+ ++  LP+L+L VTTS GVDH+DL E RRRGI+VA AG+ +SEDVAD
Sbjct: 71  AIIAPVAA-PVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVAD 130

Query: 121 MAVGLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEG 180
            AVGLLIDV R++SAA+RF++Q  WP   ++PLG KL  KRIGIVGLG IG +VA RL+ 
Sbjct: 131 TAVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGSKLGRKRIGIVGLGSIGSKVATRLDA 190

Query: 181 FGCKMSYNSRTKKPL-VPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKD 240
           FGC++SY+SR +KP  VPY Y+  + E+AA  +ALI+CC L E+T  +I+++V+ ALGK 
Sbjct: 191 FGCQISYSSRNRKPYDVPYHYYMDIEEMAANSDALIICCELNEKTLRLINKDVLSALGKR 250

Query: 241 GVIINVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVIT 300
           GVI+NV RGAIIDE+E++ CL +GEIGGAGLDVFE+EP +P+ELF +DNVV SPH A +T
Sbjct: 251 GVIVNVARGAIIDEEEMVRCLREGEIGGAGLDVFEDEPNVPKELFELDNVVFSPHSAFMT 310

Query: 301 HESMKGLCKLVVDNLEAFFSNKPLVSPFM 327
            E ++ L K+VV N+EAFFSNKPL++P +
Sbjct: 311 LEGLEELGKVVVGNIEAFFSNKPLLTPVL 338

BLAST of MC05g0305 vs. TAIR 10
Match: AT1G12550.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 333.2 bits (853), Expect = 2.4e-91
Identity = 167/314 (53.18%), Postives = 224/314 (71.34%), Query Frame = 0

Query: 12  PQVLVLGSPWVLPSLESQFSHRFH-FLKPSNSQFPLLQFLSSYAQSVQALLIPGGSFALT 71
           P V++L  P  L  ++   +  F   +  ++S   L  F   +A S +A +I  G   +T
Sbjct: 7   PPVVLLHRPPSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVI-SGRLPVT 66

Query: 72  SAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAVGLLIDVLRK 131
             +L  LPSL++ V TSVG+DH+DL   +RRGI +  AGN +S+DVAD AVGLLI VLR+
Sbjct: 67  DELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRR 126

Query: 132 VSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGCKMSYNSRTK 191
           + AADR++R G W    +F LG K+SGKR+GIVGLG IG  VAKRLE FGC +SYNSR++
Sbjct: 127 IPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQ 186

Query: 192 KPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVIINVGRGAIID 251
           K   PY Y+S +  LA   + L++CC LT+ET H+++REVM  LGKDGV+INVGRG +ID
Sbjct: 187 KQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLID 246

Query: 252 EKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESMKGLCKLVVD 311
           EKE+++CL+ G IGGAGLDVFENEP +P+ELFG+DNVVLSPH AV T  S+  + ++ + 
Sbjct: 247 EKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALA 306

Query: 312 NLEAFFSNKPLVSP 325
           NL+AFFSN+PL+SP
Sbjct: 307 NLKAFFSNRPLLSP 319

BLAST of MC05g0305 vs. TAIR 10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 265.4 bits (677), Expect = 6.1e-71
Identity = 137/299 (45.82%), Postives = 200/299 (66.89%), Query Frame = 0

Query: 26  LESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALLIPGGSFALTSAILDCLPSLKLAVT 85
           LE++   RF+ L+   S    +  L ++  S++A ++   S    + ++  LP+L++  +
Sbjct: 16  LENELEKRFNLLRFWTSPEKSV-LLETHRNSIRA-VVGNASAGADAQLISDLPNLEIVSS 75

Query: 86  TSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAVGLLIDVLRKVSAADRFLRQGLWPT 145
            SVG+D +DL + + +GI V    ++ +EDVAD+A+GL++ +LR++   DR++R G W  
Sbjct: 76  FSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDRYVRSGKWKQ 135

Query: 146 NREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGCKMSYNSRTKKPLVPYSYHSTVHEL 205
             EF L  K SGK +GI+GLG+IG  +AKR E F C ++Y SRT KP V Y Y+ TV +L
Sbjct: 136 G-EFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPDVAYKYYPTVVDL 195

Query: 206 AAECEALIVCCGLTEETRHMIDREVMVALGKDGVIINVGRGAIIDEKELIECLLQGEIGG 265
           A   + L+V C LTE+TRH++DR+VM ALG  GV+IN+GRG  +DE+ELI+ L +G +GG
Sbjct: 196 AQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGRLGG 255

Query: 266 AGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESMKGLCKLVVDNLEAFFSNKPLVSP 325
           A LDVFE EP +PEELFG++NVVL PHV   T E+   +  LVV NLEA FS K L++P
Sbjct: 256 AALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLEAHFSGKSLLTP 311

BLAST of MC05g0305 vs. TAIR 10
Match: AT1G79870.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 235.7 bits (600), Expect = 5.2e-62
Identity = 126/299 (42.14%), Postives = 187/299 (62.54%), Query Frame = 0

Query: 26  LESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALLIPGGSFALTSAILDCLPSLKLAVT 85
           LE++   RF+ L+   S    +  L ++  S++A ++   S    + ++  LP+L++  +
Sbjct: 16  LENELEKRFNLLRFWTSPEKSV-LLETHRNSIRA-VVGNASAGADAQLISDLPNLEIVSS 75

Query: 86  TSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAVGLLIDVLRKVSAADRFLRQGLWPT 145
            SVG+D +DL + + +GI V    ++ +EDVAD+A+GL++ +LR++   DR++R G W  
Sbjct: 76  FSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDRYVRSGKWKQ 135

Query: 146 NREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGCKMSYNSRTKKPLVPYSYHSTVHEL 205
                               G+IG  +AKR E F C ++Y SRT KP V Y Y+ TV +L
Sbjct: 136 --------------------GRIGTAIAKRAEAFSCPINYYSRTIKPDVAYKYYPTVVDL 195

Query: 206 AAECEALIVCCGLTEETRHMIDREVMVALGKDGVIINVGRGAIIDEKELIECLLQGEIGG 265
           A   + L+V C LTE+TRH++DR+VM ALG  GV+IN+GRG  +DE+ELI+ L +G +GG
Sbjct: 196 AQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGRLGG 255

Query: 266 AGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESMKGLCKLVVDNLEAFFSNKPLVSP 325
           A LDVFE EP +PEELFG++NVVL PHV   T E+   +  LVV NLEA FS K L++P
Sbjct: 256 AALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLEAHFSGKSLLTP 292

BLAST of MC05g0305 vs. TAIR 10
Match: AT2G45630.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 139.4 bits (350), Expect = 5.0e-33
Identity = 74/157 (47.13%), Postives = 108/157 (68.79%), Query Frame = 0

Query: 1   SMATKIHGDELPQVLVLGSPWVLPSLESQF--SHRFHFLKPSNSQFPLLQFLSSYAQSVQ 60
           +MAT    ++LP+VL++  P  +  L   F  S +F  LK   S  PL +FL+ ++ S+ 
Sbjct: 11  TMATSSSTEKLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSIS 70

Query: 61  ALLIPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVAD 120
           A++ P  +  +T+ ++  LP+L+L VTTS GVDH+DL E RRRGI+VA AG+ +SEDVAD
Sbjct: 71  AIIAPVAA-PVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVAD 130

Query: 121 MAVGLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKL 156
            AVGLLIDV R++SAA+RF++Q  WP   ++PLG K+
Sbjct: 131 TAVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGSKV 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LE333.3e-9053.18Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR... [more]
A5CAL12.7e-7146.20Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN... [more]
Q65CJ74.5e-7145.93Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR ... [more]
Q9CA908.6e-7045.82Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=H... [more]
Q5FTU62.8e-5239.572-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GO... [more]
Match NameE-valueIdentityDescription
XP_022159358.12.20e-234100.00glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia][more]
KAG6597176.11.01e-18979.94Glyoxylate/hydroxypyruvate reductase HPR3, partial [Cucurbita argyrosperma subsp... [more]
XP_022950894.11.18e-18880.25glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita moschata][more]
XP_023539654.12.38e-18880.25glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita pepo subsp. pepo][more]
XP_022147257.17.89e-18882.04glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1DYK81.07e-234100.00glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1GH555.72e-18980.25glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1D1V93.82e-18882.04glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1I9J92.12e-18478.09glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1D0731.98e-17777.30glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
AT2G45630.22.9e-9754.10D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G12550.12.4e-9153.18D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G79870.16.1e-7145.82D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G79870.25.2e-6242.14D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT2G45630.15.0e-3347.13D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 54..321
e-value: 1.5E-16
score: 60.8
NoneNo IPR availableGENE3D3.40.50.720coord: 113..297
e-value: 2.3E-101
score: 340.4
NoneNo IPR availableGENE3D3.40.50.720coord: 29..320
e-value: 2.3E-101
score: 340.4
NoneNo IPR availablePANTHERPTHR109962-HYDROXYACID DEHYDROGENASE-RELATEDcoord: 9..325
NoneNo IPR availablePANTHERPTHR10996:SF179D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN-RELATEDcoord: 9..325
NoneNo IPR availableCDDcd12156HPPRcoord: 12..317
e-value: 3.30158E-146
score: 411.862
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 14..147
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 121..294
e-value: 2.1E-49
score: 167.3
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 113..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0305.1MC05g0305.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor