MC05g0284 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0284 vs. ExPASy Swiss-Prot
Match: Q5EAR5 (tRNA 2'-phosphotransferase 1 OS=Danio rerio OX=7955 GN=trpt1 PE=2 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 1.2e-32
Identity = 85/200 (42.50%), Postives = 123/200 (61.50%), Query Frame = 0

Query: 71  RTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFAN 130
           R RGR G G  + + +   L + L+ +LRH AS++ L M SDG+V V++LL       A+
Sbjct: 6   RGRGRRGRGNRNEESRDVRLSKSLSYVLRHGASKMGLQMNSDGFVFVEELL-------AH 65

Query: 131 IPLRSHTIDDIREAVRKDNKQRFGLLE--ENGELLIRANQGHTVTVVETESLLRPILSAE 190
              RS ++DD+   V  ++KQRF L +  E+  L IRANQGH+V V + E  LR I   +
Sbjct: 66  QQFRSFSVDDVERVVASNDKQRFKLCKHPEDDRLQIRANQGHSVQVTDLE--LREISQDD 125

Query: 191 E--VPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGMRRDVNVLIFLDV 250
           +      VHGSY K+  SI   GL RM R H+H + GLP +G VISGMR+  ++ +++DV
Sbjct: 126 QDYPREAVHGSYMKHWPSIRSQGLSRMNRTHIHLAPGLPGEGRVISGMRQSCDLAVYIDV 185

Query: 251 KKALDDGMKLYVSDNKVILT 267
            KA+ DG+K + S+N V+LT
Sbjct: 186 TKAMSDGIKFFWSENGVLLT 196

BLAST of MC05g0284 vs. ExPASy Swiss-Prot
Match: Q8K3A2 (tRNA 2'-phosphotransferase 1 OS=Mus musculus OX=10090 GN=Trpt1 PE=2 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 1.0e-26
Identity = 78/186 (41.94%), Postives = 111/186 (59.68%), Query Frame = 0

Query: 84  KDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIRE 143
           +D+   L + L+  LRH A +L L MR+DG+V +Q LL+L           S +I+D++ 
Sbjct: 20  QDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLP-------QFHSFSIEDVQL 79

Query: 144 AVRKDNKQRFGLL--EENGELLIRANQGHTVTVVETESLLRPILSAEEVPV-CVHGSYKK 203
            V  + KQRF L   E +  LLIRANQGH++ V E E  L P+ + + +P+  VHG++ K
Sbjct: 80  VVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELE--LTPLETPQALPLTLVHGTFWK 139

Query: 204 NLESILESGLKRMKRLHVHFSCGLPTDGEVISGMRRDVNVLIFLDVKKALDDGMKLYVSD 263
           +  SIL  GL R  R H+H + GLP D  VISG+R +  V +F+D   AL DG+  + S 
Sbjct: 140 HWPSILLKGLSRQGRTHIHLASGLPGDPGVISGIRPNCEVAVFIDGPLALTDGIPFFCSA 196

Query: 264 NKVILT 267
           N VILT
Sbjct: 200 NGVILT 196

BLAST of MC05g0284 vs. ExPASy Swiss-Prot
Match: O14045 (Putative tRNA 2'-phosphotransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC2C4.12c PE=3 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 3.9e-26
Identity = 68/176 (38.64%), Postives = 104/176 (59.09%), Query Frame = 0

Query: 92  RLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQ 151
           + L+++LRH A    L +R DGY++V  +LKL          R   ++ +   V+ ++K+
Sbjct: 43  KALSKVLRHTAKANGLQIREDGYIEVDSILKLP-------QFRGMGMELLLSIVKGNDKK 102

Query: 152 RFGLLEENGELLIRANQGHTVTVVETESLLRPILSAEEVPVCVHGSYKKNLESILESGLK 211
           RF + E  G L IRANQGH++  V+    +  I +A  +P  VHG+ K+    I + GL 
Sbjct: 103 RFTMEEVEGVLYIRANQGHSIKAVQVP--MARIDNASSIPKVVHGTKKELWPVISKQGLS 162

Query: 212 RMKRLHVHFSCGLPTDGEVISGMRRDVNVLIFLDVKKALDDGMKLYVSDNKVILTE 268
           RMKR H+H + GL  D  VISG+R+   + I++D  KA+ DG++ Y S+N VILTE
Sbjct: 163 RMKRNHIHCATGLYGDPGVISGIRKSCTLYIYIDSAKAMQDGVEFYRSENGVILTE 209

BLAST of MC05g0284 vs. ExPASy Swiss-Prot
Match: Q86TN4 (tRNA 2'-phosphotransferase 1 OS=Homo sapiens OX=9606 GN=TRPT1 PE=1 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 6.9e-23
Identity = 71/186 (38.17%), Postives = 106/186 (56.99%), Query Frame = 0

Query: 84  KDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIRE 143
           +D+   L + L+  LRH A +L L M +DG+V +  LL+L          R  + +D++ 
Sbjct: 25  QDRDVQLSKALSYALRHGALKLGLPMGADGFVPLGTLLQLP-------QFRGFSAEDVQR 84

Query: 144 AVRKDNKQRFGLL--EENGELLIRANQGHTVTVVETESLLRPILSAEEV-PVCVHGSYKK 203
            V  + KQRF L   + +  LLIRANQGH++ V + E  L P+ + + + P+ VHG++ K
Sbjct: 85  VVDTNRKQRFALQLGDPSTGLLIRANQGHSLQVPKLE--LMPLETPQALPPMLVHGTFWK 144

Query: 204 NLESILESGLKRMKRLHVHFSCGLPTDGEVISGMRRDVNVLIFLDVKKALDDGMKLYVSD 263
           +  SIL  GL    R H+H + GLP D  +ISGMR    + +F+D   AL DG+  + S 
Sbjct: 145 HWPSILLKGLSCQGRTHIHLAPGLPGDPGIISGMRSHCEIAVFIDGPLALADGIPFFRSA 201

Query: 264 NKVILT 267
           N VILT
Sbjct: 205 NGVILT 201

BLAST of MC05g0284 vs. ExPASy Swiss-Prot
Match: Q3ZBM7 (tRNA 2'-phosphotransferase 1 OS=Bos taurus OX=9913 GN=TRPT1 PE=2 SV=3)

HSP 1 Score: 106.3 bits (264), Expect = 1.3e-21
Identity = 74/197 (37.56%), Postives = 111/197 (56.35%), Query Frame = 0

Query: 73  RGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFANIP 132
           +GR        +D+   L + L+  LRH A +L L M +DG+V +  LL+L         
Sbjct: 15  KGRRAHRPPQDQDRDVQLSKALSYALRHGALKLGLPMGADGFVPLDALLQLP-------Q 74

Query: 133 LRSHTIDDIREAVRKDNKQRFGLL--EENGELLIRANQGHTVTVVETESLLRPILSAEEV 192
            RS + +D++  V  + KQRF L   + +   LIRANQGH++ V E E  L P+ + + +
Sbjct: 75  FRSFSAEDVQRVVDTNVKQRFALQPGDPSTGPLIRANQGHSLQVPELE--LEPLETPQAL 134

Query: 193 P-VCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGMRRDVNVLIFLDVKKA 252
           P + VHG+++++  SIL  GL    R H+H + GLP D  VISGMR +  V +F++   A
Sbjct: 135 PLMLVHGTFRQHWPSILLKGLSCRGRTHIHLAPGLPGDPGVISGMRPNCEVAVFINGPLA 194

Query: 253 LDDGMKLYVSDNKVILT 267
           L DG+  + S N VILT
Sbjct: 195 LADGIPFFRSTNGVILT 202

BLAST of MC05g0284 vs. NCBI nr
Match: KAA0049045.1 (Phosphotransferase KptA/Tpt1 [Cucumis melo var. makuwa])

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 518/684 (75.73%), Postives = 555/684 (81.14%), Query Frame = 0

Query: 1   MWACV-TNCGIKILRYRPLLLPRVLITPIPLPIPPAVFSMDNPNTSSFASFAHSSKSGGR 60
           MWA V TNCGIKILR R LLLPRVLITPIPLPI P++ SMD  NT+S +SFAHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GRGLD+ NDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR+DGYVKV+D
Sbjct: 61  GRGLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRF LL+ENGELLIRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGMRRDVN 240
           LL+PILSAEEVPVCVHG+YKKNLESIL+SGLKRMKRLHVHFSCGLPTDGEVISGMRRDVN
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISGMRRDVN 240

Query: 241 VLIFLDVKKALDDGMKLYVSDNKVILTEEKRKKKQKNGGQYRSISGGVRRLRSLSSMRIC 300
           VLIFLDV+KAL+DGMKLY+SDN+VILTE                                
Sbjct: 241 VLIFLDVQKALEDGMKLYISDNRVILTE-------------------------------- 300

Query: 301 SERRRFLFFLPLVFFIPHLLSVLELHHGLTSQDQPNKRTKKFDHLVLGPAAGQGYADRLQ 360
                                VLELHH L S+ QP+K++KKFDHL+LGPAAGQG +DRLQ
Sbjct: 301 ---------------------VLELHHDLASK-QPDKQSKKFDHLILGPAAGQGLSDRLQ 360

Query: 361 CSGTKALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKAVGRSSDIVKVGDASYSKV 420
           CSGTKALNNTH P TS+SA SGD+IHFVTVFTIYNAS DSK +GRS+D+VKVGDASYSKV
Sbjct: 361 CSGTKALNNTHSPDTSNSAESGDSIHFVTVFTIYNASQDSKVIGRSTDVVKVGDASYSKV 420

Query: 421 ERSMAILNVFINFIR--------------------------------------------- 480
           ERSMA+LNVFI+FI+                                             
Sbjct: 421 ERSMAVLNVFIDFIQVSMPQSNVVILTDPASDLPVRRNRVAVFPIQGEYSRDTLMLQRIR 480

Query: 481 ---RYLDARLEEQRQVPRHTYHYIFTDSDMAVVGDLGEIFRQCPKFHLALTFRNNKAQPL 540
               +LDA+L+EQRQ  RH  HYIFTDSDMAVVGDLGEIFR+ PKFHLALTFRNNKAQPL
Sbjct: 481 SYISFLDAKLDEQRQGTRHINHYIFTDSDMAVVGDLGEIFRKHPKFHLALTFRNNKAQPL 540

Query: 541 NSGFIAVRGTEDGILRAKAFLQEVLRIYSSKFMKASRMLGDQLALAWVVRSNPSFDARKF 600
           NSGFIAVRGTEDGILRAKAFL+EVL+IYSSKFMKASRMLGDQLALAWVV+SNPSFDARKF
Sbjct: 541 NSGFIAVRGTEDGILRAKAFLEEVLKIYSSKFMKASRMLGDQLALAWVVKSNPSFDARKF 600

Query: 601 SKAETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGSRKRLMLESWNF 635
           SK ETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGSRKRLMLESWNF
Sbjct: 601 SKPETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGSRKRLMLESWNF 630

BLAST of MC05g0284 vs. NCBI nr
Match: KAA8515925.1 (hypothetical protein F0562_019104 [Nyssa sinensis])

HSP 1 Score: 811 bits (2096), Expect = 1.51e-288
Identity = 445/696 (63.94%), Postives = 509/696 (73.13%), Query Frame = 0

Query: 1   MWACVTNCGIKILRYRPLLLPRVLITPIPLPIPP----------AVFSMDNPNT--SSFA 60
           MWA +T  G++ILR   + LP     P P               A+ SMD  +T  SSF+
Sbjct: 1   MWASITCGGLRILRNFCVSLP-----PFPFAASSTSSSLLTSFRALASMDKTDTKSSSFS 60

Query: 61  SFAHSSKSGGRGRGLDMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLN 120
           S  HS +  GRGRG +M ++RER+RG G GG    KDKIDALGRLLTR+LRHMASELNLN
Sbjct: 61  SSIHSGRRDGRGRGSEMKDNRERSRGSGSGG----KDKIDALGRLLTRVLRHMASELNLN 120

Query: 121 MRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQ 180
           MRSDGYV+VQDLLKLN+KTFANIPLR HT+DDIREAVRKDNKQRF LLEENGELLIRANQ
Sbjct: 121 MRSDGYVRVQDLLKLNVKTFANIPLRLHTVDDIREAVRKDNKQRFSLLEENGELLIRANQ 180

Query: 181 GHTVTVVETESLLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDG 240
           GHT+T VETESLL+PILSAEEVPVCVHG+YKK LESILE GLKRMKRLHVHFSCGLPTDG
Sbjct: 181 GHTITTVETESLLKPILSAEEVPVCVHGTYKKFLESILEHGLKRMKRLHVHFSCGLPTDG 240

Query: 241 EVISGMRRDVNVLIFLDVKKALDDGMKLYVSDNKVILTEEKRKKKQKNGGQYRSISGGVR 300
           EVISG+RRDVNVLIFLDV+KAL++GMKLY+SDNK           QK             
Sbjct: 241 EVISGLRRDVNVLIFLDVRKALEEGMKLYISDNK---------SPQK------------- 300

Query: 301 RLRSLSSMRICSERRRFLFFLPLVFFIPHLLSVLELHHGLTSQDQPNKRTKKFDHLVLGP 360
               L  MR CSE RRFL   PL+FF+PH+ SVLELH   T +D   K+TKKFDHLVLGP
Sbjct: 301 ---ILLGMRACSEWRRFLLCFPLIFFLPHIFSVLELHQKTTMEDLHKKQTKKFDHLVLGP 360

Query: 361 AAGQGYADRLQCSGTKALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKAVGRSSDI 420
           AAG+G  DRLQC G KALN T     S+++N G++I FVTVFT Y++S+D    GRS D+
Sbjct: 361 AAGEGLHDRLQCQGIKALNKT-----SNNSNFGESIAFVTVFTTYSSSIDKPVSGRSRDL 420

Query: 421 VKVGDASYSKVERSMAILNVFINFIR---------------------------------- 480
           V VG+ SY KVERSMAILNVFINFI+                                  
Sbjct: 421 VTVGNISYDKVERSMAILNVFINFIQVTMPKSNIFILTDPASTLPVHRNRVTLYPIQGQY 480

Query: 481 --------------RYLDARLEEQRQVPRHTYHYIFTDSDMAVVGDLGEIFRQCPKFHLA 540
                          +L+ RL+E  Q   H  HYIFTDSD+AVVG+LG+IF   PKFHLA
Sbjct: 481 SRDKLMLQRIKSYIAFLETRLKEHSQQQEHINHYIFTDSDIAVVGNLGKIFYNYPKFHLA 540

Query: 541 LTFRNNKAQPLNSGFIAVRGTEDGILRAKAFLQEVLRIYSSKFMKASRMLGDQLALAWVV 600
           LTFRNNK QPLNSGFIAVRGT DGILRAK FLQEVL++Y+SK+MKASRMLGDQLALAWVV
Sbjct: 541 LTFRNNKEQPLNSGFIAVRGTRDGILRAKDFLQEVLKVYNSKYMKASRMLGDQLALAWVV 600

Query: 601 RSNPSFDARKFSKAETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGS 635
           +S+ SF+ ++F++ + F+E+I GASVLFLPCA+YNWTPPEGAGQFHGMPL+VKVVHFKGS
Sbjct: 601 KSHASFNVKRFTRPQAFLEDIGGASVLFLPCAIYNWTPPEGAGQFHGMPLDVKVVHFKGS 657

BLAST of MC05g0284 vs. NCBI nr
Match: OMP00624.1 (Phosphotransferase KptA/Tpt1 [Corchorus olitorius])

HSP 1 Score: 795 bits (2053), Expect = 9.77e-282
Identity = 436/702 (62.11%), Postives = 509/702 (72.51%), Query Frame = 0

Query: 1   MWACVTNCGIKILRYRPLLLPRVLITPIPLPIPPAVF-----------SMDNPNTSSFAS 60
           MWA +TN   +ILRY  L LP         P+P  V            +++N  +SSF+S
Sbjct: 1   MWATLTNGSFRILRYCVLRLP---------PVPSQVHRFRALTFMENSNLNNVTSSSFSS 60

Query: 61  FAHSSKSG--------GRGRGLDMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHM 120
           FAHSSKSG        GRGRG +M +D+ER+ GRGGG GG  KDKIDALGRLLTRILRHM
Sbjct: 61  FAHSSKSGDRGRGRGRGRGRGREMNHDQERSGGRGGGSGG--KDKIDALGRLLTRILRHM 120

Query: 121 ASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGE 180
           A+ELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHT+D+IREAVR+DNKQRF LLEENGE
Sbjct: 121 AAELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTVDEIREAVRRDNKQRFSLLEENGE 180

Query: 181 LLIRANQGHTVTVVETESLLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFS 240
           LLIRANQGHTVT+VE+ESLL+ ILSA+EV VCVHG+YK+NLESILESGLKRMKRLHVHFS
Sbjct: 181 LLIRANQGHTVTIVESESLLKEILSADEVQVCVHGTYKRNLESILESGLKRMKRLHVHFS 240

Query: 241 CGLPTDGEVISGMRRDVNVLIFLDVKKALDDGMKLYVSDNKVILTEEKRKKKQKNGGQYR 300
            GLPTDGEVISGMRRDVN+LIFL+V+KAL++GMKLY+SDNKVILTE          G   
Sbjct: 241 SGLPTDGEVISGMRRDVNILIFLNVRKALEEGMKLYISDNKVILTE----------GFDG 300

Query: 301 SISGGVRRLRSLSSMRICSERRRFLFFLPLVFFIPHLLSVLELHHGLTSQDQPNKRTKKF 360
           ++   + R+ S  +  I S R   L +LPL         V ELH  L   +   K   KF
Sbjct: 301 AVPVKIFRIDSPKTSFIASYRENRLIYLPLS------SPVRELHQDLAVGNASKKNVNKF 360

Query: 361 DHLVLGPAAGQGYADRLQCSGTKALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKA 420
           DHLVLGPAAG+G  +RL C G KALN TH    SH++  G+ I FVTVFTIYN S ++  
Sbjct: 361 DHLVLGPAAGEGLPNRLHCQGVKALNKTHFSNPSHASGVGNGIAFVTVFTIYNNSPEALL 420

Query: 421 VGRSSDIVKVGDASYSKVERSMAILNVFINFIR--------------------------- 480
             R +++V VG+ASYSK ERSMAILNVFINFI+                           
Sbjct: 421 NSRLANLVTVGNASYSKSERSMAILNVFINFIQVRMPQSNVIILTDPESDLSLHQNRVTV 480

Query: 481 ---------------------RYLDARLEEQRQVPRHTYHYIFTDSDMAVVGDLGEIFRQ 540
                                 +L+ RLE+  Q      H+IFTDSD+AV+ DLG+IF +
Sbjct: 481 QPIEGEYSRDKLMLQRIRSYITFLNLRLEKLSQEQGRITHFIFTDSDIAVIDDLGQIFDK 540

Query: 541 CPKFHLALTFRNNKAQPLNSGFIAVRGTEDGILRAKAFLQEVLRIYSSKFMKASRMLGDQ 600
              FH+ALTFRNNK QPLNSGFIAVRGT DGILRAK FLQ+VL +YSS++MKASRMLGDQ
Sbjct: 541 YQDFHMALTFRNNKDQPLNSGFIAVRGTHDGILRAKHFLQKVLEVYSSRYMKASRMLGDQ 600

Query: 601 LALAWVVRSNPSFDARKFSKAETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVK 635
           LALAWV++S+PSFDA++FSKA+ F+EE+ GASVLFLPCA+YNWTPPEGAGQFHGMPL+VK
Sbjct: 601 LALAWVIKSHPSFDAKRFSKAQAFIEEVGGASVLFLPCAVYNWTPPEGAGQFHGMPLDVK 660

BLAST of MC05g0284 vs. NCBI nr
Match: EEF50363.1 (conserved hypothetical protein [Ricinus communis])

HSP 1 Score: 783 bits (2021), Expect = 8.80e-279
Identity = 404/594 (68.01%), Postives = 467/594 (78.62%), Query Frame = 0

Query: 43  NTSSFASFA-HSSKSGGRGRGLDMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHM 102
           N+SSF+S + HSS+SGG G                  GGG  KDKIDALGRLLTRILRHM
Sbjct: 2   NSSSFSSSSPHSSRSGGDG------------------GGGGAKDKIDALGRLLTRILRHM 61

Query: 103 ASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGE 162
           ASELNLNMRSDG+V+VQDLLKLN+KTFA+IP+RSHT+DDI+EAVR+DNKQRF LLEENGE
Sbjct: 62  ASELNLNMRSDGFVRVQDLLKLNMKTFASIPIRSHTVDDIKEAVRRDNKQRFSLLEENGE 121

Query: 163 LLIRANQGHTVTVVETESLLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFS 222
           LLIRANQGHT+  VE+ESLL+PI+ A+EVPVCVHG+YKKNLESILESGLKRM+RLH+HFS
Sbjct: 122 LLIRANQGHTIKTVESESLLKPIILADEVPVCVHGTYKKNLESILESGLKRMQRLHIHFS 181

Query: 223 CGLPTDGEVISGMRRDVNVLIFLDVKKALDDGMKLYVSDNKVILTEEKRKKKQKNGGQYR 282
           CGLPTDGEVISGMRR+VNVLI+LDV+KAL+DGMKLY+SDN+VILTE              
Sbjct: 182 CGLPTDGEVISGMRRNVNVLIYLDVRKALEDGMKLYISDNRVILTE-------------- 241

Query: 283 SISGGVRRLRSLSSMRICSERRRFLFFLPLVFFIPHLLSVLELHHGLTSQDQPNKRTKKF 342
              G V          +  ER      + L  +  H+ SVLELH    ++       KK 
Sbjct: 242 GFDGVV---------PVNYERPLLFCVMYLACYTMHVASVLELHWNSVTEAPQKNWNKKS 301

Query: 343 DHLVLGPAAGQGYADRLQCSGTKALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKA 402
           DHLV+GPAAGQG  DRLQC G+KALN THL  +S  +N GDN+ FVTVFTIYN SLDS  
Sbjct: 302 DHLVIGPAAGQGLPDRLQCEGSKALNKTHLLDSSSGSNVGDNVAFVTVFTIYNTSLDSLP 361

Query: 403 VGRSSDIVKVGDASYSKVERSMAILNVFINFIRRYLDARLEEQRQVPRHTYHYIFTDSDM 462
             RSS++V VG+ SYSK ERSMAILNVFINFI+ +LD +L+E  + P H  HYIFTDSD+
Sbjct: 362 DDRSSNLVTVGNVSYSKTERSMAILNVFINFIQNFLDTKLKELAKNPVHKSHYIFTDSDI 421

Query: 463 AVVGDLGEIFRQCPKFHLALTFRNNKAQPLNSGFIAVRGTEDGILRAKAFLQEVLRIYSS 522
           AVV DLG IF + P FH+ALTFRNNK QPLNSGFIAVRGT + ILRAK FLQ VL +Y+S
Sbjct: 422 AVVDDLGRIFHEYPNFHIALTFRNNKEQPLNSGFIAVRGTAESILRAKIFLQHVLEVYTS 481

Query: 523 KFMKASRMLGDQLALAWVVRSNPSFDARKFSKAETFVEEINGASVLFLPCALYNWTPPEG 582
           K+M ASRMLGDQLALAWV+RS+P FD ++F KA+ F++E+ GASVLFLPCA YNWTPPEG
Sbjct: 482 KYMNASRMLGDQLALAWVIRSHPGFDLQRFRKAQAFMDEMGGASVLFLPCATYNWTPPEG 541

Query: 583 AGQFHGMPLNVKVVHFKGSRKRLMLESWNFFRSSSNISDMLCLILSSGRTKYDF 635
           AGQFHGMPL+VKVVHFKGSRK LMLESWNFFRS+S+ISDMLCLIL SGRTKYDF
Sbjct: 542 AGQFHGMPLDVKVVHFKGSRKHLMLESWNFFRSASDISDMLCLILMSGRTKYDF 554

BLAST of MC05g0284 vs. NCBI nr
Match: KAF3439994.1 (hypothetical protein FNV43_RR18272 [Rhamnella rubrinervis])

HSP 1 Score: 775 bits (2001), Expect = 2.04e-274
Identity = 429/692 (61.99%), Postives = 489/692 (70.66%), Query Frame = 0

Query: 1   MWACVTNCGIKILRYRPLLLPRVLITPIPLPI-PPAVFSMDNPNTSS-----FASFAHSS 60
           MWA V++  I+IL Y  +LLPRV  TP    I  PA+  MDN N SS     F S A  +
Sbjct: 1   MWASVSSSSIRILSYCRVLLPRVSATPTLQGIGSPALVFMDNTNNSSSGNSSFVSSARPN 60

Query: 61  KSGGRGRGLDMTNDRERTRGR-GGGGGGS--GKDKIDALGRLLTRILRHMASELNLNMRS 120
             GGR R LDM NDRER+RGR GGGGGGS  GKDKIDALGRL+TRILRHMASELNLN+RS
Sbjct: 61  GRGGRERDLDMKNDRERSRGRRGGGGGGSSVGKDKIDALGRLMTRILRHMASELNLNVRS 120

Query: 121 DGYVKVQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGHT 180
           DGYVKV DLLKLN+KTFANIPL+SHT+DDIREAVRKD+KQRF LLEENGELLIRANQGHT
Sbjct: 121 DGYVKVHDLLKLNMKTFANIPLKSHTVDDIREAVRKDSKQRFSLLEENGELLIRANQGHT 180

Query: 181 VTVVETESLLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVI 240
           +T VE+E LL+PILS EEVPVCVHG+Y+KNLESIL SGLKRMKRLHVHFSCGLP DGEVI
Sbjct: 181 ITAVESERLLKPILSVEEVPVCVHGTYRKNLESILASGLKRMKRLHVHFSCGLPADGEVI 240

Query: 241 SGMRRDVNVLIFLDVKKALDDGMKLYVSDNKVILTEEKRKKKQKNGGQYRSISGGVRRLR 300
           SGMRRDVN++IFLDVKKAL++GMKLY+SDNKVILT                         
Sbjct: 241 SGMRRDVNIMIFLDVKKALEEGMKLYISDNKVILT------------------------- 300

Query: 301 SLSSMRICSERRRFLFFLPLVFFIPHLLSVLELHHGLTSQDQPNKRTKKFDHLVLGPAAG 360
                                       +V+ELH     +D   K++KK DHLVLGPAAG
Sbjct: 301 ----------------------------AVMELHQKAAVEDVLKKQSKKSDHLVLGPAAG 360

Query: 361 QGYADRLQCSGTKALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKAVGRSSDIVKV 420
           QG  +RLQC GT+AL  TH    S +++ GD +  VTVFTIYN+SL   A   S ++V V
Sbjct: 361 QGLPNRLQCRGTRALIKTHFSVPSKASDIGDTVSLVTVFTIYNSSLIVHA-DNSLNLVTV 420

Query: 421 GDASYSKVERSMAILNVFINFIR------------------------------------- 480
           G+ASY+K ERSMAILN FINFI+                                     
Sbjct: 421 GNASYNKEERSMAILNAFINFIQLTMPQSNIIILTDPVSDLAIRRNRVTVYPIQGEYSRD 480

Query: 481 -----------RYLDARLEEQRQVPRHTYHYIFTDSDMAVVGDLGEIFRQCPKFHLALTF 540
                       +LD R+EE  Q      +YIFTDSD+AVVGDLG IF   P FH+ALTF
Sbjct: 481 KLMLQRIRSYIAFLDTRIEELSQRQGPICNYIFTDSDIAVVGDLGHIFCDFPNFHVALTF 540

Query: 541 RNNKAQPLNSGFIAVRGTEDGILRAKAFLQEVLRIYSSKFMKASRMLGDQLALAWVVRSN 600
           RNNKAQPLNSGFIAVRGT +G+LRAK FLQEVL++Y SK+  +SRMLGDQLALAWVVRSN
Sbjct: 541 RNNKAQPLNSGFIAVRGTHNGLLRAKIFLQEVLKVYISKYSSSSRMLGDQLALAWVVRSN 600

Query: 601 PSFDARKFSKAETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGSRKR 635
           PSFD ++FSKA+ F+E++ G SVLFLPCA +NWTPPEGAGQFHGMPL+VKVVHFKGSRKR
Sbjct: 601 PSFDTKRFSKAQPFLEDVGGTSVLFLPCATHNWTPPEGAGQFHGMPLDVKVVHFKGSRKR 638

BLAST of MC05g0284 vs. ExPASy TrEMBL
Match: A0A5A7U5Z1 (2'-phosphotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002720 PE=3 SV=1)

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 518/684 (75.73%), Postives = 555/684 (81.14%), Query Frame = 0

Query: 1   MWACV-TNCGIKILRYRPLLLPRVLITPIPLPIPPAVFSMDNPNTSSFASFAHSSKSGGR 60
           MWA V TNCGIKILR R LLLPRVLITPIPLPI P++ SMD  NT+S +SFAHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GRGLD+ NDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR+DGYVKV+D
Sbjct: 61  GRGLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRF LL+ENGELLIRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGMRRDVN 240
           LL+PILSAEEVPVCVHG+YKKNLESIL+SGLKRMKRLHVHFSCGLPTDGEVISGMRRDVN
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISGMRRDVN 240

Query: 241 VLIFLDVKKALDDGMKLYVSDNKVILTEEKRKKKQKNGGQYRSISGGVRRLRSLSSMRIC 300
           VLIFLDV+KAL+DGMKLY+SDN+VILTE                                
Sbjct: 241 VLIFLDVQKALEDGMKLYISDNRVILTE-------------------------------- 300

Query: 301 SERRRFLFFLPLVFFIPHLLSVLELHHGLTSQDQPNKRTKKFDHLVLGPAAGQGYADRLQ 360
                                VLELHH L S+ QP+K++KKFDHL+LGPAAGQG +DRLQ
Sbjct: 301 ---------------------VLELHHDLASK-QPDKQSKKFDHLILGPAAGQGLSDRLQ 360

Query: 361 CSGTKALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKAVGRSSDIVKVGDASYSKV 420
           CSGTKALNNTH P TS+SA SGD+IHFVTVFTIYNAS DSK +GRS+D+VKVGDASYSKV
Sbjct: 361 CSGTKALNNTHSPDTSNSAESGDSIHFVTVFTIYNASQDSKVIGRSTDVVKVGDASYSKV 420

Query: 421 ERSMAILNVFINFIR--------------------------------------------- 480
           ERSMA+LNVFI+FI+                                             
Sbjct: 421 ERSMAVLNVFIDFIQVSMPQSNVVILTDPASDLPVRRNRVAVFPIQGEYSRDTLMLQRIR 480

Query: 481 ---RYLDARLEEQRQVPRHTYHYIFTDSDMAVVGDLGEIFRQCPKFHLALTFRNNKAQPL 540
               +LDA+L+EQRQ  RH  HYIFTDSDMAVVGDLGEIFR+ PKFHLALTFRNNKAQPL
Sbjct: 481 SYISFLDAKLDEQRQGTRHINHYIFTDSDMAVVGDLGEIFRKHPKFHLALTFRNNKAQPL 540

Query: 541 NSGFIAVRGTEDGILRAKAFLQEVLRIYSSKFMKASRMLGDQLALAWVVRSNPSFDARKF 600
           NSGFIAVRGTEDGILRAKAFL+EVL+IYSSKFMKASRMLGDQLALAWVV+SNPSFDARKF
Sbjct: 541 NSGFIAVRGTEDGILRAKAFLEEVLKIYSSKFMKASRMLGDQLALAWVVKSNPSFDARKF 600

Query: 601 SKAETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGSRKRLMLESWNF 635
           SK ETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGSRKRLMLESWNF
Sbjct: 601 SKPETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGSRKRLMLESWNF 630

BLAST of MC05g0284 vs. ExPASy TrEMBL
Match: A0A5J4ZBE3 (2'-phosphotransferase OS=Nyssa sinensis OX=561372 GN=F0562_019104 PE=3 SV=1)

HSP 1 Score: 811 bits (2096), Expect = 7.32e-289
Identity = 445/696 (63.94%), Postives = 509/696 (73.13%), Query Frame = 0

Query: 1   MWACVTNCGIKILRYRPLLLPRVLITPIPLPIPP----------AVFSMDNPNT--SSFA 60
           MWA +T  G++ILR   + LP     P P               A+ SMD  +T  SSF+
Sbjct: 1   MWASITCGGLRILRNFCVSLP-----PFPFAASSTSSSLLTSFRALASMDKTDTKSSSFS 60

Query: 61  SFAHSSKSGGRGRGLDMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLN 120
           S  HS +  GRGRG +M ++RER+RG G GG    KDKIDALGRLLTR+LRHMASELNLN
Sbjct: 61  SSIHSGRRDGRGRGSEMKDNRERSRGSGSGG----KDKIDALGRLLTRVLRHMASELNLN 120

Query: 121 MRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQ 180
           MRSDGYV+VQDLLKLN+KTFANIPLR HT+DDIREAVRKDNKQRF LLEENGELLIRANQ
Sbjct: 121 MRSDGYVRVQDLLKLNVKTFANIPLRLHTVDDIREAVRKDNKQRFSLLEENGELLIRANQ 180

Query: 181 GHTVTVVETESLLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDG 240
           GHT+T VETESLL+PILSAEEVPVCVHG+YKK LESILE GLKRMKRLHVHFSCGLPTDG
Sbjct: 181 GHTITTVETESLLKPILSAEEVPVCVHGTYKKFLESILEHGLKRMKRLHVHFSCGLPTDG 240

Query: 241 EVISGMRRDVNVLIFLDVKKALDDGMKLYVSDNKVILTEEKRKKKQKNGGQYRSISGGVR 300
           EVISG+RRDVNVLIFLDV+KAL++GMKLY+SDNK           QK             
Sbjct: 241 EVISGLRRDVNVLIFLDVRKALEEGMKLYISDNK---------SPQK------------- 300

Query: 301 RLRSLSSMRICSERRRFLFFLPLVFFIPHLLSVLELHHGLTSQDQPNKRTKKFDHLVLGP 360
               L  MR CSE RRFL   PL+FF+PH+ SVLELH   T +D   K+TKKFDHLVLGP
Sbjct: 301 ---ILLGMRACSEWRRFLLCFPLIFFLPHIFSVLELHQKTTMEDLHKKQTKKFDHLVLGP 360

Query: 361 AAGQGYADRLQCSGTKALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKAVGRSSDI 420
           AAG+G  DRLQC G KALN T     S+++N G++I FVTVFT Y++S+D    GRS D+
Sbjct: 361 AAGEGLHDRLQCQGIKALNKT-----SNNSNFGESIAFVTVFTTYSSSIDKPVSGRSRDL 420

Query: 421 VKVGDASYSKVERSMAILNVFINFIR---------------------------------- 480
           V VG+ SY KVERSMAILNVFINFI+                                  
Sbjct: 421 VTVGNISYDKVERSMAILNVFINFIQVTMPKSNIFILTDPASTLPVHRNRVTLYPIQGQY 480

Query: 481 --------------RYLDARLEEQRQVPRHTYHYIFTDSDMAVVGDLGEIFRQCPKFHLA 540
                          +L+ RL+E  Q   H  HYIFTDSD+AVVG+LG+IF   PKFHLA
Sbjct: 481 SRDKLMLQRIKSYIAFLETRLKEHSQQQEHINHYIFTDSDIAVVGNLGKIFYNYPKFHLA 540

Query: 541 LTFRNNKAQPLNSGFIAVRGTEDGILRAKAFLQEVLRIYSSKFMKASRMLGDQLALAWVV 600
           LTFRNNK QPLNSGFIAVRGT DGILRAK FLQEVL++Y+SK+MKASRMLGDQLALAWVV
Sbjct: 541 LTFRNNKEQPLNSGFIAVRGTRDGILRAKDFLQEVLKVYNSKYMKASRMLGDQLALAWVV 600

Query: 601 RSNPSFDARKFSKAETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGS 635
           +S+ SF+ ++F++ + F+E+I GASVLFLPCA+YNWTPPEGAGQFHGMPL+VKVVHFKGS
Sbjct: 601 KSHASFNVKRFTRPQAFLEDIGGASVLFLPCAIYNWTPPEGAGQFHGMPLDVKVVHFKGS 657

BLAST of MC05g0284 vs. ExPASy TrEMBL
Match: A0A1R3K0M5 (2'-phosphotransferase OS=Corchorus olitorius OX=93759 GN=COLO4_12531 PE=3 SV=1)

HSP 1 Score: 795 bits (2053), Expect = 4.73e-282
Identity = 436/702 (62.11%), Postives = 509/702 (72.51%), Query Frame = 0

Query: 1   MWACVTNCGIKILRYRPLLLPRVLITPIPLPIPPAVF-----------SMDNPNTSSFAS 60
           MWA +TN   +ILRY  L LP         P+P  V            +++N  +SSF+S
Sbjct: 1   MWATLTNGSFRILRYCVLRLP---------PVPSQVHRFRALTFMENSNLNNVTSSSFSS 60

Query: 61  FAHSSKSG--------GRGRGLDMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHM 120
           FAHSSKSG        GRGRG +M +D+ER+ GRGGG GG  KDKIDALGRLLTRILRHM
Sbjct: 61  FAHSSKSGDRGRGRGRGRGRGREMNHDQERSGGRGGGSGG--KDKIDALGRLLTRILRHM 120

Query: 121 ASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGE 180
           A+ELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHT+D+IREAVR+DNKQRF LLEENGE
Sbjct: 121 AAELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTVDEIREAVRRDNKQRFSLLEENGE 180

Query: 181 LLIRANQGHTVTVVETESLLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFS 240
           LLIRANQGHTVT+VE+ESLL+ ILSA+EV VCVHG+YK+NLESILESGLKRMKRLHVHFS
Sbjct: 181 LLIRANQGHTVTIVESESLLKEILSADEVQVCVHGTYKRNLESILESGLKRMKRLHVHFS 240

Query: 241 CGLPTDGEVISGMRRDVNVLIFLDVKKALDDGMKLYVSDNKVILTEEKRKKKQKNGGQYR 300
            GLPTDGEVISGMRRDVN+LIFL+V+KAL++GMKLY+SDNKVILTE          G   
Sbjct: 241 SGLPTDGEVISGMRRDVNILIFLNVRKALEEGMKLYISDNKVILTE----------GFDG 300

Query: 301 SISGGVRRLRSLSSMRICSERRRFLFFLPLVFFIPHLLSVLELHHGLTSQDQPNKRTKKF 360
           ++   + R+ S  +  I S R   L +LPL         V ELH  L   +   K   KF
Sbjct: 301 AVPVKIFRIDSPKTSFIASYRENRLIYLPLS------SPVRELHQDLAVGNASKKNVNKF 360

Query: 361 DHLVLGPAAGQGYADRLQCSGTKALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKA 420
           DHLVLGPAAG+G  +RL C G KALN TH    SH++  G+ I FVTVFTIYN S ++  
Sbjct: 361 DHLVLGPAAGEGLPNRLHCQGVKALNKTHFSNPSHASGVGNGIAFVTVFTIYNNSPEALL 420

Query: 421 VGRSSDIVKVGDASYSKVERSMAILNVFINFIR--------------------------- 480
             R +++V VG+ASYSK ERSMAILNVFINFI+                           
Sbjct: 421 NSRLANLVTVGNASYSKSERSMAILNVFINFIQVRMPQSNVIILTDPESDLSLHQNRVTV 480

Query: 481 ---------------------RYLDARLEEQRQVPRHTYHYIFTDSDMAVVGDLGEIFRQ 540
                                 +L+ RLE+  Q      H+IFTDSD+AV+ DLG+IF +
Sbjct: 481 QPIEGEYSRDKLMLQRIRSYITFLNLRLEKLSQEQGRITHFIFTDSDIAVIDDLGQIFDK 540

Query: 541 CPKFHLALTFRNNKAQPLNSGFIAVRGTEDGILRAKAFLQEVLRIYSSKFMKASRMLGDQ 600
              FH+ALTFRNNK QPLNSGFIAVRGT DGILRAK FLQ+VL +YSS++MKASRMLGDQ
Sbjct: 541 YQDFHMALTFRNNKDQPLNSGFIAVRGTHDGILRAKHFLQKVLEVYSSRYMKASRMLGDQ 600

Query: 601 LALAWVVRSNPSFDARKFSKAETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVK 635
           LALAWV++S+PSFDA++FSKA+ F+EE+ GASVLFLPCA+YNWTPPEGAGQFHGMPL+VK
Sbjct: 601 LALAWVIKSHPSFDAKRFSKAQAFIEEVGGASVLFLPCAVYNWTPPEGAGQFHGMPLDVK 660

BLAST of MC05g0284 vs. ExPASy TrEMBL
Match: B9RDA5 (2'-phosphotransferase OS=Ricinus communis OX=3988 GN=RCOM_1611600 PE=3 SV=1)

HSP 1 Score: 783 bits (2021), Expect = 4.26e-279
Identity = 404/594 (68.01%), Postives = 467/594 (78.62%), Query Frame = 0

Query: 43  NTSSFASFA-HSSKSGGRGRGLDMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHM 102
           N+SSF+S + HSS+SGG G                  GGG  KDKIDALGRLLTRILRHM
Sbjct: 2   NSSSFSSSSPHSSRSGGDG------------------GGGGAKDKIDALGRLLTRILRHM 61

Query: 103 ASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGE 162
           ASELNLNMRSDG+V+VQDLLKLN+KTFA+IP+RSHT+DDI+EAVR+DNKQRF LLEENGE
Sbjct: 62  ASELNLNMRSDGFVRVQDLLKLNMKTFASIPIRSHTVDDIKEAVRRDNKQRFSLLEENGE 121

Query: 163 LLIRANQGHTVTVVETESLLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFS 222
           LLIRANQGHT+  VE+ESLL+PI+ A+EVPVCVHG+YKKNLESILESGLKRM+RLH+HFS
Sbjct: 122 LLIRANQGHTIKTVESESLLKPIILADEVPVCVHGTYKKNLESILESGLKRMQRLHIHFS 181

Query: 223 CGLPTDGEVISGMRRDVNVLIFLDVKKALDDGMKLYVSDNKVILTEEKRKKKQKNGGQYR 282
           CGLPTDGEVISGMRR+VNVLI+LDV+KAL+DGMKLY+SDN+VILTE              
Sbjct: 182 CGLPTDGEVISGMRRNVNVLIYLDVRKALEDGMKLYISDNRVILTE-------------- 241

Query: 283 SISGGVRRLRSLSSMRICSERRRFLFFLPLVFFIPHLLSVLELHHGLTSQDQPNKRTKKF 342
              G V          +  ER      + L  +  H+ SVLELH    ++       KK 
Sbjct: 242 GFDGVV---------PVNYERPLLFCVMYLACYTMHVASVLELHWNSVTEAPQKNWNKKS 301

Query: 343 DHLVLGPAAGQGYADRLQCSGTKALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKA 402
           DHLV+GPAAGQG  DRLQC G+KALN THL  +S  +N GDN+ FVTVFTIYN SLDS  
Sbjct: 302 DHLVIGPAAGQGLPDRLQCEGSKALNKTHLLDSSSGSNVGDNVAFVTVFTIYNTSLDSLP 361

Query: 403 VGRSSDIVKVGDASYSKVERSMAILNVFINFIRRYLDARLEEQRQVPRHTYHYIFTDSDM 462
             RSS++V VG+ SYSK ERSMAILNVFINFI+ +LD +L+E  + P H  HYIFTDSD+
Sbjct: 362 DDRSSNLVTVGNVSYSKTERSMAILNVFINFIQNFLDTKLKELAKNPVHKSHYIFTDSDI 421

Query: 463 AVVGDLGEIFRQCPKFHLALTFRNNKAQPLNSGFIAVRGTEDGILRAKAFLQEVLRIYSS 522
           AVV DLG IF + P FH+ALTFRNNK QPLNSGFIAVRGT + ILRAK FLQ VL +Y+S
Sbjct: 422 AVVDDLGRIFHEYPNFHIALTFRNNKEQPLNSGFIAVRGTAESILRAKIFLQHVLEVYTS 481

Query: 523 KFMKASRMLGDQLALAWVVRSNPSFDARKFSKAETFVEEINGASVLFLPCALYNWTPPEG 582
           K+M ASRMLGDQLALAWV+RS+P FD ++F KA+ F++E+ GASVLFLPCA YNWTPPEG
Sbjct: 482 KYMNASRMLGDQLALAWVIRSHPGFDLQRFRKAQAFMDEMGGASVLFLPCATYNWTPPEG 541

Query: 583 AGQFHGMPLNVKVVHFKGSRKRLMLESWNFFRSSSNISDMLCLILSSGRTKYDF 635
           AGQFHGMPL+VKVVHFKGSRK LMLESWNFFRS+S+ISDMLCLIL SGRTKYDF
Sbjct: 542 AGQFHGMPLDVKVVHFKGSRKHLMLESWNFFRSASDISDMLCLILMSGRTKYDF 554

BLAST of MC05g0284 vs. ExPASy TrEMBL
Match: A0A6A1W148 (2'-phosphotransferase OS=Morella rubra OX=262757 GN=CJ030_MR3G015115 PE=3 SV=1)

HSP 1 Score: 764 bits (1974), Expect = 2.45e-271
Identity = 406/619 (65.59%), Postives = 460/619 (74.31%), Query Frame = 0

Query: 65  MTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLN 124
           M NDRER+RGRG  GGGSGKDKIDALGRLLTRILRHMASEL LNMRSDGYV VQDLLKLN
Sbjct: 1   MANDRERSRGRG--GGGSGKDKIDALGRLLTRILRHMASELCLNMRSDGYVNVQDLLKLN 60

Query: 125 LKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGHTVTVVETESLLRPI 184
           +KTFANIPLRSHT+DDIREAVR+DNKQRF LLEENGELLIRANQGHTVT VE+E LL+PI
Sbjct: 61  MKTFANIPLRSHTVDDIREAVRRDNKQRFSLLEENGELLIRANQGHTVTTVESERLLKPI 120

Query: 185 LSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGMRRDVNVLIFL 244
           +SAEEV VC+HG+Y+KNLESILESGLKRMKRLHVHFSCGLPTDG+VISGMRRDVNVLIFL
Sbjct: 121 VSAEEVSVCIHGTYRKNLESILESGLKRMKRLHVHFSCGLPTDGQVISGMRRDVNVLIFL 180

Query: 245 DVKKALDDGMKLYVSDNKVILTEEKRKKKQKNGGQYRSISGGVRRLRSLSSMRICSERRR 304
           DV+KAL++GMKLY+SDN VILTE                 G V     ++  R    R +
Sbjct: 181 DVRKALEEGMKLYISDNNVILTE--------------GFDGVVPVKSKIAWCRTDELRIK 240

Query: 305 FLFFLPLVFFIPHLLSVLELHHGLTSQDQPNKRTKKFDHLVLGPAAGQGYADRLQCSGTK 364
                        ++ +  +    T +     + KKFDHLVLGPAAGQG  +RLQC G K
Sbjct: 241 G----------AEIIDITLIVLSPTVEYPARNKNKKFDHLVLGPAAGQGLRNRLQCEGIK 300

Query: 365 ALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKAVGRSSDIVKVGDASYSKVERSMA 424
           +LN TH  T+S  A  G+N+ FVTVFTI+N+ L+ +   RSSD+V VG+ASYSK +RSMA
Sbjct: 301 SLNKTHFSTSSSIARGGENVAFVTVFTIFNSPLNVRVDDRSSDLVTVGNASYSKAQRSMA 360

Query: 425 ILNVFINFIR------------------------------------------------RY 484
           ILNVFINFI+                                                 +
Sbjct: 361 ILNVFINFIQVTMPQSNIVILTDPASDLNLHRDRVNIHPIPGEYSRDKLMLQRIRAYIAF 420

Query: 485 LDARLEEQRQVPRHTYHYIFTDSDMAVVGDLGEIFRQCPKFHLALTFRNNKAQPLNSGFI 544
           L+ RLEE  +   H  HYIFTDSD+AVV DLG+IF   P FHLALTFRNNKAQPLNSGFI
Sbjct: 421 LEVRLEELSRKEGHITHYIFTDSDIAVVEDLGQIFEDYPNFHLALTFRNNKAQPLNSGFI 480

Query: 545 AVRGTEDGILRAKAFLQEVLRIYSSKFMKASRMLGDQLALAWVVRSNPSFDARKFSKAET 604
           AVRGT DGI RAK FLQ VL +Y+SK+M ASRMLGDQLALAWVV+S+PSFD RKF+KA+ 
Sbjct: 481 AVRGTADGISRAKLFLQVVLEVYTSKYMSASRMLGDQLALAWVVKSDPSFDTRKFTKAQA 540

Query: 605 FVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGSRKRLMLESWNFFRSSS 635
           F+EEI  ASVLFLPCA+YNWTPPEGAGQFHGMPL+VKVVHFKGSRKRLMLESWNFFRSS+
Sbjct: 541 FLEEIGDASVLFLPCAIYNWTPPEGAGQFHGMPLDVKVVHFKGSRKRLMLESWNFFRSSA 593

BLAST of MC05g0284 vs. TAIR 10
Match: AT2G45320.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 10 growth stages; Has 45 Blast hits to 45 proteins in 16 species: Archae - 0; Bacteria - 8; Metazoa - 0; Fungi - 0; Plants - 37; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 408.3 bits (1048), Expect = 1.1e-113
Identity = 218/394 (55.33%), Postives = 264/394 (67.01%), Query Frame = 0

Query: 291 RSLSSMRICSERRRFLFFLPLVFFIPHLLSVLEL-HHGLTSQDQPNKRTKKFDHLVLGPA 350
           R   SMR CS  RR L  +P++F +PHL S+++      T  D      KK DHLVLGP 
Sbjct: 5   RDYRSMRSCSGWRRILLLIPVLFLLPHLSSLVDFSSDSATRNDARTIPNKKLDHLVLGPV 64

Query: 351 AGQGYADRLQCSGTKALNNTHLPTTSHSANSGDNIHFVTVFTIYNASLDSKAVGRSSDIV 410
           AGQG +DR  C GTKALN TH  +TSH + +G+ + FVTVFT+YN SL +  V  S+ + 
Sbjct: 65  AGQGLSDRFHCRGTKALNKTH-GSTSHVSGAGNGVSFVTVFTVYNTSLGN--VKSSNPVS 124

Query: 411 KVGDASYSKVERSMAILNVFINFIR----------------------------------- 470
            VG+ +YSK ERSMA+LN F NFI+                                   
Sbjct: 125 VVGNVTYSKPERSMAVLNAFANFIQVTMPKSNVVILTDPASDLSIQQSNVILQPVQGDYS 184

Query: 471 -------------RYLDARLEEQRQVPRHTYHYIFTDSDMAVVGDLGEIFRQCPKFHLAL 530
                         +L+ +LE+         HYIFTDSD+AVV D+G IF +   FHLAL
Sbjct: 185 RGNLMLQRIRSYITFLEMKLEKNE---GGINHYIFTDSDIAVVDDVGTIFDKHSSFHLAL 244

Query: 531 TFRNNKAQPLNSGFIAVRGTEDGILRAKAFLQEVLRIYSSKFMKASRMLGDQLALAWVVR 590
           TFRNNK QPLNSGFIAVRGT +GILRAK FL+EVL+ Y +K+MKASRMLGDQLAL  VV+
Sbjct: 245 TFRNNKDQPLNSGFIAVRGTREGILRAKVFLEEVLKAYKTKYMKASRMLGDQLALVSVVK 304

Query: 591 SNPSFDARKFSKAETFVEEINGASVLFLPCALYNWTPPEGAGQFHGMPLNVKVVHFKGSR 636
           S+ SFDA++F+K + F EEI GASVLFLPCALYNWTPPEGAGQFHGMPL+VK+VHFKGSR
Sbjct: 305 SHASFDAKRFTKPQAFTEEIAGASVLFLPCALYNWTPPEGAGQFHGMPLDVKIVHFKGSR 364

BLAST of MC05g0284 vs. TAIR 10
Match: AT2G45330.1 (RNA 2'-phosphotransferase, Tpt1 / KptA family )

HSP 1 Score: 347.4 bits (890), Expect = 2.4e-95
Identity = 181/264 (68.56%), Postives = 215/264 (81.44%), Query Frame = 0

Query: 10  IKILRYR---PLLLPRVLITPIPLPIPPAVFSMDNPNT---SSFASFAHSSKSGGRGRGL 69
           +++ R+R   PL L   +      P+  A     NPN+   S+ +SFA SS+SGGRG G 
Sbjct: 3   LRLCRHRLPLPLTLSSSVFFSKSSPVSSAFMDASNPNSSRKSNVSSFAQSSRSGGRGGGY 62

Query: 70  DMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKL 129
           +  NDR R +GRG GGG  GKD+IDALGRLLTRILRHMA+EL LNMR DG+VKV+DLL L
Sbjct: 63  ERDNDRRRPQGRGDGGG--GKDRIDALGRLLTRILRHMATELRLNMRGDGFVKVEDLLNL 122

Query: 130 NLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGHTVTVVETESLLRP 189
           NLKT ANI L+SHTID+IREAVR+DNKQRF L++ENGELLIRANQGH++T VE+E LL+P
Sbjct: 123 NLKTSANIQLKSHTIDEIREAVRRDNKQRFSLIDENGELLIRANQGHSITTVESEKLLKP 182

Query: 190 ILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGMRRDVNVLIF 249
           ILS EE PVCVHG+Y+KNLESIL SGLKRM R+HVHFSCGLPTDGEVISGMRR+VNV+IF
Sbjct: 183 ILSPEEAPVCVHGTYRKNLESILASGLKRMNRMHVHFSCGLPTDGEVISGMRRNVNVIIF 242

Query: 250 LDVKKALDDGMKLYVSDNKVILTE 268
           LD+KKAL+DG+  Y+SDNKVILTE
Sbjct: 243 LDIKKALEDGIAFYISDNKVILTE 264

BLAST of MC05g0284 vs. TAIR 10
Match: AT2G45330.2 (RNA 2'-phosphotransferase, Tpt1 / KptA family )

HSP 1 Score: 336.7 bits (862), Expect = 4.2e-92
Identity = 174/261 (66.67%), Postives = 210/261 (80.46%), Query Frame = 0

Query: 10  IKILRYR---PLLLPRVLITPIPLPIPPAVFSMDNPNTSSFASFAHSSKSGGRGRGLDMT 69
           +++ R+R   PL L   +      P+  A     NPN+S  ++ +  ++S  RG G +  
Sbjct: 3   LRLCRHRLPLPLTLSSSVFFSKSSPVSSAFMDASNPNSSRKSNVSSFAQSSRRGGGYERD 62

Query: 70  NDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLK 129
           NDR R +GRG GGG  GKD+IDALGRLLTRILRHMA+EL LNMR DG+VKV+DLL LNLK
Sbjct: 63  NDRRRPQGRGDGGG--GKDRIDALGRLLTRILRHMATELRLNMRGDGFVKVEDLLNLNLK 122

Query: 130 TFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGHTVTVVETESLLRPILS 189
           T ANI L+SHTID+IREAVR+DNKQRF L++ENGELLIRANQGH++T VE+E LL+PILS
Sbjct: 123 TSANIQLKSHTIDEIREAVRRDNKQRFSLIDENGELLIRANQGHSITTVESEKLLKPILS 182

Query: 190 AEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGMRRDVNVLIFLDV 249
            EE PVCVHG+Y+KNLESIL SGLKRM R+HVHFSCGLPTDGEVISGMRR+VNV+IFLD+
Sbjct: 183 PEEAPVCVHGTYRKNLESILASGLKRMNRMHVHFSCGLPTDGEVISGMRRNVNVIIFLDI 242

Query: 250 KKALDDGMKLYVSDNKVILTE 268
           KKAL+DG+  Y+SDNKVILTE
Sbjct: 243 KKALEDGIAFYISDNKVILTE 261

BLAST of MC05g0284 vs. TAIR 10
Match: AT5G23600.1 (RNA 2'-phosphotransferase, Tpt1 / KptA family )

HSP 1 Score: 294.7 bits (753), Expect = 1.8e-79
Identity = 140/183 (76.50%), Postives = 166/183 (90.71%), Query Frame = 0

Query: 85  DKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREA 144
           D+IDALGR+LTRILRHMA+EL LNMR DG+VKV+DLL LNLKT ANI L SHTID+IREA
Sbjct: 5   DRIDALGRILTRILRHMATELRLNMRGDGFVKVEDLLNLNLKTCANIQLNSHTIDEIREA 64

Query: 145 VRKDNKQRFGLLEENGELLIRANQGHTVTVVETESLLRPILSAEEVPVCVHGSYKKNLES 204
           V +DNK+RF L++E+GELLIRANQGH++T VE+E LL+PILS EE PVCVHG+Y+KNLES
Sbjct: 65  VTRDNKKRFSLIDEDGELLIRANQGHSITTVESEKLLKPILSPEEAPVCVHGTYRKNLES 124

Query: 205 ILESGLKRMKRLHVHFSCGLPTDGEVISGMRRDVNVLIFLDVKKALDDGMKLYVSDNKVI 264
           IL SGLKRM R+HVHFSCGLPTDGEVISG+RR+VNV+IFL +KKAL+DG+  Y+SDNKVI
Sbjct: 125 ILASGLKRMNRMHVHFSCGLPTDGEVISGVRRNVNVIIFLHIKKALEDGIAFYISDNKVI 184

Query: 265 LTE 268
           LT+
Sbjct: 185 LTQ 187