MC05g0275 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0275
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pectinesterase
Location	MC05: 2020479 .. 2023492 (+)
RNA-Seq Expression	MC05g0275
Synteny	MC05g0275

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTACCAAAATGCCCTCTCCCTTCGCGGCCTTTCTTCTCCTCCTCCTAGCCGTGGCTTCCTCAATTTCGTCATTCCACGACCCAGACCGGGAGGTTCAGTTGGCCCGGACCATAGTTATGGAGGCCAGAGACTCGGTCCGAGGCTCCGTGACCGGGACAGGTGAGTTAAAACGACGCCGCTTTGAACGGCGGAATTTTGTTGAGGCCGCGCTCGGTTATTGCACCAGGCTTTACGAGGAGGCCGACGCGCGGCTGGCGGAGCTGCTGTTCGGAGGAAATTATACGGCGGAAGATGCTCGGACGTGGATCAGCGCCGCCGTGGCTAATCATCGGAGTTGTACGGACGGCCTGGAAGAGGTCGGAGCTGCGGCGGATAGGAAGAATAATTTGACTGCGTTACTCGCCGGAGCTCTGCGTTTATACGAGAAAATCGCCGCCGTGGAGGAGCGTAATGGTAACCACACGTACTTCCGACGTTGCATTCATTCGGATATTCGAAGTTATTAGTTTTCTATTTAAATATATCACTGAAAATCGTTTTTTTTTTTTTCTTTTTTGTTTCGTTTCGTATCTTTATTCGTGGTCGTCTTAATGTTCAAAATTAATTGTTTTCTATGTTTTTAGTCTACTAATAATAAATATGGTACTAAAAAAATACCTGAAAATATTTGACTCCTGTCTTTACAAAATTGACCAACTTATTATTATTTTCAGACAATTCACTATTATTTTCTTTGAGTATTTAGGTTTGGATTTCTCTCTTTTACCTATCAAATATTTAGGGACAAGAACCATCTAAAATCTAAAATGTGATCGAATATTTAGGGATAAAGAACAATCTAAAAAAGTATCCATCTTGCTGTTAGGTTTACTAGAATTTTCACTTTTAAAATTGAAGTTTTGCAAACATTTTGTGAAGCATTGATAAACTATCCTTCCCAAAGCTTAAAATTGGCTTAAATTGATGGGTTGATAAATTGAATTCAATATATCTCGATTGATGGGTTGGGTATATATAAATATTTTATAAAATATATTATAAAAATTGCACAACGATAAGAACTCATTTAATAATGATGTGATTTATATGTTTACTACATTGGTGATAAAATAAAGTATATTAACACTTTATCTATAGATGATGTGTCTATAGATTTATGTATTGTATAAATCATACATAAAGCGTAAGTCAATTAGTTATTTAAGCATTTGTCGGTAAGATTGTAAGTTCCAATTCTCACACCTACTTAAATGTGAGTGAGTGTGATAGATAAGCATATGTATTTGAATGAGTGTGATGGATAAGCATGTATTTTTCTACTTATTTAGCAACTAGTTTACCCTTAAAATATAAAAAATAAAAAATAAAATAAAATATCCTTATAAGAATTAAGTTGTCACCTAATTAGCGCAACGAGCATAGTCAATTTAAATACATATTTCTGAACAAAAAGTCATGCGTTTCAACTCTCCCAACATGTTGTATCTCATGAAAAAAAGTTGTCGCCTAATTGTATTTTTCTCACGTCTCAATGTATTTATTTAAAAGATACATATGGACTTTTGAATTCAGGATTGAAACGATGGGAGGAGCAGAAATTGACGAAAAATAGAGGAATCGACTTGGCGAAATGGAATCCGGCGACGTCGAAGGCGGACTATGTGGTGGCGATGGACGGCTCCGGCACACACAGAACCATAAACGACGCGGTGGCGGCTCTGGCGAGGAGTGGCCGGAGACGGCGGTCGGAGCGAGTGGTGATTTACGTGAAAGCCGGAGTTTACAGGGAGAACGTGGAAATCGGGATGCAATTGAAGAATGTGTTTATTGTCGGCGACGGCGCCGACAGAACCACCATCACCGGCAGCCGCAACGTGCCGGACGGCGCCACCACTTACAGCTCAGCAACATTTGGTGAGGCGAAACGTATTTATCACAAAACAATAAAATATTATTTAATAATATTGAAAATAATAATAAAATATATAAAGGTTTATGGAGGCATTTAAAATTAATGAAGCTTACAAAATTAACTTTGATGAAACTTTGGTATGAGCAGGCGTCTCCGGCGACGGATTTTGGGCAAGGGACATCACATTCGAGAACACCGCCGGGCCGGAAAAGCACCAGGCGGTGGCGCTGCGGGTAAATTCCGACCTCGCCGTCGTCTACCGCTGCACAATCAAAGGCTACCAGGACTCCCTCTTCCTCCACTCCCTCCGCCAATTCTACCGCGACTGCCGCATATACGGCACCGTCGATTTCATCTTCGGAAACTCCGCCGCCGTCCTCCAGAACTGCGAAATCTTCGTCCGGCGGCCGATGCGCCACCAAGCCAACATGATCACCGCCCATGGCCGCGACGATCCGGCGGAGAACACCGGAATTTCGATCCACGGCTCGCGAGTCAGGGCGGCACCGGAATTCGCCGCCGTACAGGGCGAATTCAGAACGTTCCTCGGCCGGCCGTGGAAGCGGTACTCGAGGACGGTGGTTATGGCGACGGATCTGGAGGGATTGATCGATCCGCGTGGGTGGGGAGAATGGGAAGGGGATTTTCCTCTGTCGACTCTGTTTTATGGTGAATATAAGAACACCGGCCGCGGTTCTTCCACTCGATTTAGGGTTAATTGGCCCGGATTTCACATTTTTCGGGGCGATCTGGAGGCCGGAAGGTTTACGGTCGGCGGTTTTCTCCACGGCCGCGATTGGATTCCGGTGACCGGAGTGCCCTTCCAGGCAGGAATTCGAGCTCTTGGTCCTCTGCTTGAAAATATATATATATACACGTGGAATTTGAATAGCGATAAAAATCATAATGAGAGGGACTAAATAAATAATGAAATTAAAAATAAAAATATATAAAGCACGCCCAGTGGGACTCGAACCCACAATCGCCTGATTAGAAGTCAGACGCCTTATCCATTAGGCCATGGGCGCTGTTTGAAACAACAACCATTTATATTTTATATATTATAATTATGTAATTTTCCATTATATCTTATAAAAATAG

mRNA sequence

ATTACCAAAATGCCCTCTCCCTTCGCGGCCTTTCTTCTCCTCCTCCTAGCCGTGGCTTCCTCAATTTCGTCATTCCACGACCCAGACCGGGAGGTTCAGTTGGCCCGGACCATAGTTATGGAGGCCAGAGACTCGGTCCGAGGCTCCGTGACCGGGACAGGTGAGTTAAAACGACGCCGCTTTGAACGGCGGAATTTTGTTGAGGCCGCGCTCGGTTATTGCACCAGGCTTTACGAGGAGGCCGACGCGCGGCTGGCGGAGCTGCTGTTCGGAGGAAATTATACGGCGGAAGATGCTCGGACGTGGATCAGCGCCGCCGTGGCTAATCATCGGAGTTGTACGGACGGCCTGGAAGAGGTCGGAGCTGCGGCGGATAGGAAGAATAATTTGACTGCGTTACTCGCCGGAGCTCTGCGTTTATACGAGAAAATCGCCGCCGTGGAGGAGCGTAATGGATTGAAACGATGGGAGGAGCAGAAATTGACGAAAAATAGAGGAATCGACTTGGCGAAATGGAATCCGGCGACGTCGAAGGCGGACTATGTGGTGGCGATGGACGGCTCCGGCACACACAGAACCATAAACGACGCGGTGGCGGCTCTGGCGAGGAGTGGCCGGAGACGGCGGTCGGAGCGAGTGGTGATTTACGTGAAAGCCGGAGTTTACAGGGAGAACGTGGAAATCGGGATGCAATTGAAGAATGTGTTTATTGTCGGCGACGGCGCCGACAGAACCACCATCACCGGCAGCCGCAACGTGCCGGACGGCGCCACCACTTACAGCTCAGCAACATTTGGCGTCTCCGGCGACGGATTTTGGGCAAGGGACATCACATTCGAGAACACCGCCGGGCCGGAAAAGCACCAGGCGGTGGCGCTGCGGGTAAATTCCGACCTCGCCGTCGTCTACCGCTGCACAATCAAAGGCTACCAGGACTCCCTCTTCCTCCACTCCCTCCGCCAATTCTACCGCGACTGCCGCATATACGGCACCGTCGATTTCATCTTCGGAAACTCCGCCGCCGTCCTCCAGAACTGCGAAATCTTCGTCCGGCGGCCGATGCGCCACCAAGCCAACATGATCACCGCCCATGGCCGCGACGATCCGGCGGAGAACACCGGAATTTCGATCCACGGCTCGCGAGTCAGGGCGGCACCGGAATTCGCCGCCGTACAGGGCGAATTCAGAACGTTCCTCGGCCGGCCGTGGAAGCGGTACTCGAGGACGGTGGTTATGGCGACGGATCTGGAGGGATTGATCGATCCGCGTGGGTGGGGAGAATGGGAAGGGGATTTTCCTCTGTCGACTCTGTTTTATGGTGAATATAAGAACACCGGCCGCGGTTCTTCCACTCGATTTAGGGTTAATTGGCCCGGATTTCACATTTTTCGGGGCGATCTGGAGGCCGGAAGGTTTACGGTCGGCGGTTTTCTCCACGGCCGCGATTGGATTCCGGTGACCGGAGTGCCCTTCCAGGCAGGAATTCGAGCTCTTGGTCCTCTGCTTGAAAATATATATATATACACGTGGAATTTGAATAGCGATAAAAATCATAATGAGAGGGACTAAATAAATAATGAAATTAAAAATAAAAATATATAAAGCACGCCCAGTGGGACTCGAACCCACAATCGCCTGATTAGAAGTCAGACGCCTTATCCATTAGGCCATGGGCGCTGTTTGAAACAACAACCATTTATATTTTATATATTATAATTATGTAATTTTCCATTATATCTTATAAAAATAG

Coding sequence (CDS)

ATTACCAAAATGCCCTCTCCCTTCGCGGCCTTTCTTCTCCTCCTCCTAGCCGTGGCTTCCTCAATTTCGTCATTCCACGACCCAGACCGGGAGGTTCAGTTGGCCCGGACCATAGTTATGGAGGCCAGAGACTCGGTCCGAGGCTCCGTGACCGGGACAGGTGAGTTAAAACGACGCCGCTTTGAACGGCGGAATTTTGTTGAGGCCGCGCTCGGTTATTGCACCAGGCTTTACGAGGAGGCCGACGCGCGGCTGGCGGAGCTGCTGTTCGGAGGAAATTATACGGCGGAAGATGCTCGGACGTGGATCAGCGCCGCCGTGGCTAATCATCGGAGTTGTACGGACGGCCTGGAAGAGGTCGGAGCTGCGGCGGATAGGAAGAATAATTTGACTGCGTTACTCGCCGGAGCTCTGCGTTTATACGAGAAAATCGCCGCCGTGGAGGAGCGTAATGGATTGAAACGATGGGAGGAGCAGAAATTGACGAAAAATAGAGGAATCGACTTGGCGAAATGGAATCCGGCGACGTCGAAGGCGGACTATGTGGTGGCGATGGACGGCTCCGGCACACACAGAACCATAAACGACGCGGTGGCGGCTCTGGCGAGGAGTGGCCGGAGACGGCGGTCGGAGCGAGTGGTGATTTACGTGAAAGCCGGAGTTTACAGGGAGAACGTGGAAATCGGGATGCAATTGAAGAATGTGTTTATTGTCGGCGACGGCGCCGACAGAACCACCATCACCGGCAGCCGCAACGTGCCGGACGGCGCCACCACTTACAGCTCAGCAACATTTGGCGTCTCCGGCGACGGATTTTGGGCAAGGGACATCACATTCGAGAACACCGCCGGGCCGGAAAAGCACCAGGCGGTGGCGCTGCGGGTAAATTCCGACCTCGCCGTCGTCTACCGCTGCACAATCAAAGGCTACCAGGACTCCCTCTTCCTCCACTCCCTCCGCCAATTCTACCGCGACTGCCGCATATACGGCACCGTCGATTTCATCTTCGGAAACTCCGCCGCCGTCCTCCAGAACTGCGAAATCTTCGTCCGGCGGCCGATGCGCCACCAAGCCAACATGATCACCGCCCATGGCCGCGACGATCCGGCGGAGAACACCGGAATTTCGATCCACGGCTCGCGAGTCAGGGCGGCACCGGAATTCGCCGCCGTACAGGGCGAATTCAGAACGTTCCTCGGCCGGCCGTGGAAGCGGTACTCGAGGACGGTGGTTATGGCGACGGATCTGGAGGGATTGATCGATCCGCGTGGGTGGGGAGAATGGGAAGGGGATTTTCCTCTGTCGACTCTGTTTTATGGTGAATATAAGAACACCGGCCGCGGTTCTTCCACTCGATTTAGGGTTAATTGGCCCGGATTTCACATTTTTCGGGGCGATCTGGAGGCCGGAAGGTTTACGGTCGGCGGTTTTCTCCACGGCCGCGATTGGATTCCGGTGACCGGAGTGCCCTTCCAGGCAGGAATTCGAGCTCTTGGTCCTCTGCTTGAAAATATATATATATACACGTGGAATTTGAATAGCGATAAAAATCATAATGAGAGGGACTAA

Protein sequence

ITKMPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFERRNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAAADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGIRALGPLLENIYIYTWNLNSDKNHNERD

Homology

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BLAST of MC05g0275 vs. ExPASy Swiss-Prot
Match: Q84R10 (Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana OX=3702 GN=PME36 PE=2 SV=2)

HSP 1 Score: 545.8 bits (1405), Expect = 5.3e-154
Identity = 276/512 (53.91%), Postives = 358/512 (69.92%), Query Frame = 0


Query: 12  LLLLLAVASSISSFHDPDREV-QLARTIVMEARDSVRGSVTGTGELKRRRFERRNFVEAA 71
           ++  LA+A+ I++ +  + +V ++ART V+EA+ S  GS+  T           ++ +  
Sbjct: 13  IMFFLAIAAVITASNTAELDVLEMARTAVVEAKTSF-GSMAVTEATSE---VAGSYYKLG 72

Query: 72  LGYCTRLYEEADARLAELLFG-GNYTAEDARTWISAAVANHRSCTDGL--EEVGAAADRK 131
           L  C +LY+E++ARL++L+    N+T ED RTW+S  +ANH +C DGL  +  G      
Sbjct: 73  LSECEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKPLVH 132

Query: 132 NNLTALLAGALRLYEKI---------AAVEERNGLKRWEEQKLTKNRGID---------- 191
           +N+T +L  AL  Y+K              + +G  R + +    N G            
Sbjct: 133 SNVTFVLHEALAFYKKSRGHMKKRLHGPARQGHGPTRPKHRPTRPNHGPGRSHHGPSRPN 192

Query: 192 -----LAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYR 251
                L  WNP +S+AD+VVA DGS THRTIN A+AA++R G+ R + RV+IY+KAGVY 
Sbjct: 193 QNGGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGKSRLN-RVIIYIKAGVYN 252

Query: 252 ENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTA 311
           E +EI   +KN+ +VGDG DRT +T +RNVPDG+TTY SATFGVSGDGFWARDITFENTA
Sbjct: 253 EKIEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITFENTA 312

Query: 312 GPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVL 371
           GP KHQAVALRV+SDL++ YRC+ KGYQD+LF HSLRQFYRDC IYGT+DFIFG++AAV 
Sbjct: 313 GPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDAAAVF 372

Query: 372 QNCEIFVRRPMRHQANMITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPW 431
           QNC+IFVRRPM HQ NMITA GRDDP  N+GISI  SR+RAAPEF AV+G F+++LGRPW
Sbjct: 373 QNCDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYLGRPW 432

Query: 432 KRYSRTVVMATDLEGLIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIF 491
           K+YSRTV + TD++ LIDPRGW EW G + LSTL+YGE+ NTG G+ T  RVNWPGFH+ 
Sbjct: 433 KKYSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPGFHVL 492

Query: 492 RGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 496
           RG+ EA  FTV  F+ G  WIP+TGVPF AG+
Sbjct: 493 RGEEEASPFTVSRFIQGDSWIPITGVPFSAGV 519

[Close]

BLAST of MC05g0275 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.0e-109
Identity = 230/506 (45.45%), Postives = 300/506 (59.29%), Query Frame = 0


Query: 2   TKMPSPFAAFLLLLLAVAS--SISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRR 61
           T  P P   FL     V S    + F+    ++ L R    ++R    GS        + 
Sbjct: 35  TPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALERATTAQSRTYTLGS--------KC 94

Query: 62  RFERRNFVEAALGYCTRLYEEADARLAELLFGG-NYTAEDARTWISAAVANHRSCTDGLE 121
           R ER    +AA   C  LYE    +L +        T  D +TW+S A+ N  +C   LE
Sbjct: 95  RNERE---KAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLE 154

Query: 122 EVGAAAD----RKNNLTALLAGALRLYEKIAAVEE--RNGLKRW---EEQKLTKNRGIDL 181
           ++G          NN+T L++  L L  K+   E   ++G   W    ++KL +      
Sbjct: 155 DLGVPEYVLPLLSNNVTKLISNTLSL-NKVPYNEPSYKDGFPTWVKPGDRKLLQ------ 214

Query: 182 AKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIG 241
                 T +A+ VVA DGSG  +TI +AVAA +R+G      R VIY+KAG Y EN+E+ 
Sbjct: 215 -----TTPRANIVVAQDGSGNVKTIQEAVAAASRAG----GSRYVIYIKAGTYNENIEV- 274

Query: 242 MQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQ 301
            +LKN+  VGDG  +T ITGS++V  GATT+ SAT  V GD F ARDIT  NTAGP  HQ
Sbjct: 275 -KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQ 334

Query: 302 AVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIF 361
           AVALR  SDL+V YRC+ +GYQD+L++HS RQFYR+C IYGTVDFIFGN+A VLQNC IF
Sbjct: 335 AVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIF 394

Query: 362 VRRPMRHQANMITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRT 421
            R+P  ++ N +TA GR DP ++TGI IH  RV AA +   VQ   +TFLGRPWK+YSRT
Sbjct: 395 ARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRT 454

Query: 422 VVMATDLEGLIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEA 481
           V + T L+ LI+P GW EW GDF L+TL+Y EY NTG GSST  RV W G+H+     + 
Sbjct: 455 VYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQV 510

Query: 482 GRFTVGGFLHGRDWIPVTGVPFQAGI 496
            +FTVG F+ G  W+P T VPF +G+
Sbjct: 515 SQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of MC05g0275 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 5.3e-106
Identity = 202/453 (44.59%), Postives = 272/453 (60.04%), Query Frame = 0


Query: 67  VEAALGYCTRLYEEADARLAELL-----FGGNYTAEDARTWISAAVANHRSCTDGLEE-- 126
           V +A   C  L +++   L   L       G+ +  D  TW+S+A+ NH +CTDG +E  
Sbjct: 137 VRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEIE 196

Query: 127 ----------VGAAADRK---NNLTALLAGALRLYEKIAAVEERNGLKRWEEQKL---TK 186
                     +GA  D     +N  A+ AG ++    +  V  R  L   E ++L    K
Sbjct: 197 GQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEELPNWLK 256

Query: 187 NRGIDLAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVY- 246
               +L     +  +AD  V+ DGSGT +TI +A+    +      S R VIYVKAG Y 
Sbjct: 257 REDRELLGTPTSAIQADITVSKDGSGTFKTIAEAI----KKAPEHSSRRFVIYVKAGRYE 316

Query: 247 RENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENT 306
            EN+++G +  N+  +GDG  +T ITG +++ D  TT+ +ATF  +G GF  RD+TFEN 
Sbjct: 317 EENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENY 376

Query: 307 AGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAV 366
           AGP KHQAVALRV  D AVVYRC I GYQD+L++HS RQF+R+C IYGTVDFIFGN+A +
Sbjct: 377 AGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVI 436

Query: 367 LQNCEIFVRRPMRHQANMITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRP 426
           LQ+C I+ R+PM  Q   ITA  R DP +NTGISIH  ++ A P+  A +G + T+LGRP
Sbjct: 437 LQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRP 496

Query: 427 WKRYSRTVVMATDLEGLIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHI 486
           WK YSR V M +D+   IDPRGW EW G F L +L+YGEY N G GS    RV WPG+H+
Sbjct: 497 WKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHV 556

Query: 487 FRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 496
               +EA +FTV  F+ G  W+P TGV F +G+
Sbjct: 557 ITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

[Close]

BLAST of MC05g0275 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 385.6 bits (989), Expect = 9.1e-106
Identity = 214/436 (49.08%), Postives = 276/436 (63.30%), Query Frame = 0


Query: 68  EAALGYCTRLYEEADARLAELLFGGNYTAE-DARTWISAAVANHRSCTDGLEEVGAA--- 127
           +AA   C +LY+   +++ E +      ++ DA+TW+S A+ N  +C  G  E+G     
Sbjct: 94  KAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIV 153

Query: 128 -ADRKNNLTALLAGALRLYEKIA---AVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKA 187
                NN++ LL   L +  K+       E++G   W +         D      +T K 
Sbjct: 154 LPLMSNNVSNLLCNTLAI-NKVPFNYTPPEKDGFPSWVKPG-------DRKLLQSSTPKD 213

Query: 188 DYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVG 247
           + VVA DGSG  +TI +A+ A + SG      R VIYVK GVY EN+EI  + KNV + G
Sbjct: 214 NAVVAKDGSGNFKTIKEAIDAASGSG------RFVIYVKQGVYSENLEI--RKKNVMLRG 273

Query: 248 DGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDL 307
           DG  +T ITGS++V  G TT++SAT    GDGF AR ITF NTAG    QAVALR  SDL
Sbjct: 274 DGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDL 333

Query: 308 AVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQAN 367
           +V Y+C+ + YQD+L++HS RQFYRDC +YGTVDFIFGN+AAVLQNC IF RRP R + N
Sbjct: 334 SVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTN 393

Query: 368 MITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGL 427
            ITA GR DP +NTGI IH SRV AA +   V G  +T+LGRPW++YSRTV M T L+ L
Sbjct: 394 TITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSL 453

Query: 428 IDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLH 487
           IDPRGW EW+G+F L TLFY E++NTG G+ST  RV WPGF +     EA +FTVG FL 
Sbjct: 454 IDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLA 511

Query: 488 GRDWIPVTGVPFQAGI 496
           G  WIP + VPF +G+
Sbjct: 514 GGSWIP-SSVPFTSGL 511

[Close]

BLAST of MC05g0275 vs. ExPASy Swiss-Prot
Match: O49298 (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PME6 PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.0e-104
Identity = 214/464 (46.12%), Postives = 277/464 (59.70%), Query Frame = 0


Query: 69  AALGYCTRLYEEADARL--AELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAAADR 128
           +AL  C  LYE+   +L  +   +G   +  D +T +SAA+AN  +C +G  +    +  
Sbjct: 102 SALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDFKLTSSY 161

Query: 129 K--------NNLTALLAGALRLYEKIA---AVEER---NGLKRWEEQKLTKNRGI----- 188
                     NLT  ++ +L + +  A   AV E+    G  ++ +Q+ +   G      
Sbjct: 162 SKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLL 221

Query: 189 -----DLAKWNP-----------ATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSE 248
                    W P            T+KAD VVA DGSG + +I  AV A A+    RR++
Sbjct: 222 LFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAK--LPRRNQ 281

Query: 249 RVVIYVKAGVYRENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFGVSGDG 308
           R+VIYVKAGVYRENV I   +KNV ++GDG D T +TG+RNV DG TT+ SATF VSG+G
Sbjct: 282 RLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFAVSGNG 341

Query: 309 FWARDITFENTAGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGT 368
           F A+ ITFENTAGPEKHQAVALR +SD +V Y C+ KGYQD+L+LHS RQF R+C IYGT
Sbjct: 342 FIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNIYGT 401

Query: 369 VDFIFGNSAAVLQNCEIFVRRPMRHQANMITAHGRDDPAENTGISIHGSRVRAAPEFAAV 428
           VDFIFG++ A+LQNC I+ R+PM  Q N ITA  R +P E TG  I  S V  A E    
Sbjct: 402 VDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE---- 461

Query: 429 QGEFRTFLGRPWKRYSRTVVMATDLEGLIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSST 488
                T+LGRPW+ +SRTV M  +L  L+ P GW  W G F LSTL+YGEY NTG G+S 
Sbjct: 462 -----TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASV 521

Query: 489 RFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 496
             RV WPG+H+ +   EA +FTV  FL G  WI  TGVP   G+
Sbjct: 522 SGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

[Close]

BLAST of MC05g0275 vs. NCBI nr
Match: XP_022146909.1 (pectinesterase [Momordica charantia])

HSP 1 Score: 1026 bits (2652), Expect = 0.0
Identity = 514/519 (99.04%), Postives = 514/519 (99.04%), Query Frame = 0


Query: 4   MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFER 63
           MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFER
Sbjct: 1   MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFER 60

Query: 64  RNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA 123
           RNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA
Sbjct: 61  RNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA 120

Query: 124 ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV 183
           ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV
Sbjct: 121 ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV 180

Query: 184 AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD 243
           AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD
Sbjct: 181 AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD 240

Query: 244 RTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAVVY 303
           RTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAVVY
Sbjct: 241 RTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAVVY 300

Query: 304 RCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMITA 363
           RCTIKGYQD     SLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMITA
Sbjct: 301 RCTIKGYQD-----SLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMITA 360

Query: 364 HGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPR 423
           HGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPR
Sbjct: 361 HGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPR 420

Query: 424 GWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDW 483
           GWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDW
Sbjct: 421 GWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDW 480

Query: 484 IPVTGVPFQAGIRALGPLLENIYIYTWNLNSDKNHNERD 522
           IPVTGVPFQAGIRALGPLLENIYIYTWNLNSDKNHNERD
Sbjct: 481 IPVTGVPFQAGIRALGPLLENIYIYTWNLNSDKNHNERD 514

[Close]

BLAST of MC05g0275 vs. NCBI nr
Match: XP_023540284.1 (probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 720 bits (1859), Expect = 4.04e-257
Identity = 374/495 (75.56%), Postives = 419/495 (84.65%), Query Frame = 0


Query: 4   MPSPFAAFLLLLLAV-ASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFE 63
           MP PFA  LLL LA+ ASSISS  +PD  V+L RT +++AR  +         LKRRRFE
Sbjct: 1   MPFPFATPLLLFLAIMASSISSLDEPDA-VRLLRTGIVKARSEI-------CNLKRRRFE 60

Query: 64  RRNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA 123
           R N V+ ALG C  LYEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLEEVGA
Sbjct: 61  RGN-VQTALGDCHTLYEEADSRLAGMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGA 120

Query: 124 --AADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKAD 183
             AAD  NNLT LL GAL LY+KIAAVEERNG +R E+++LT+N  I+LA WN ATSK D
Sbjct: 121 VPAADG-NNLTVLLTGALHLYDKIAAVEERNGRERVEKERLTENGRINLATWNAATSKPD 180

Query: 184 YVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGD 243
           YVVA+DGSGTH TIN AVAALAR GRRRRSERVVIYVKAGVY+ENVEIGMQLKNV +VGD
Sbjct: 181 YVVAVDGSGTHTTINGAVAALARDGRRRRSERVVIYVKAGVYKENVEIGMQLKNVMVVGD 240

Query: 244 GADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLA 303
           GAD+T +TG+RNVPDGATTYSSATFGVSGDGFWARDITFENTAGP K QAVALR+NSDLA
Sbjct: 241 GADKTIVTGNRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPGKQQAVALRLNSDLA 300

Query: 304 VVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANM 363
           VVYRCTIKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+IFVRRP+RHQANM
Sbjct: 301 VVYRCTIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQANM 360

Query: 364 ITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLI 423
           ITA GRDDPAE TGISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTVVM TDL+GLI
Sbjct: 361 ITAQGRDDPAEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVVMTTDLDGLI 420

Query: 424 DPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHG 483
           DP+GWGEW GDF +STLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV  FLHG
Sbjct: 421 DPKGWGEWRGDFAISTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAEFLHG 480

Query: 484 RDWIPVTGVPFQAGI 495
              IP TGVPF+AG+
Sbjct: 481 DACIPATGVPFEAGV 485

[Close]

BLAST of MC05g0275 vs. NCBI nr
Match: XP_022946489.1 (probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita moschata])

HSP 1 Score: 712 bits (1837), Expect = 8.96e-254
Identity = 370/495 (74.75%), Postives = 414/495 (83.64%), Query Frame = 0


Query: 4   MPSPFAAFLLLLLAV-ASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFE 63
           MP PFA  LLL +A+ A SISS  +PD  V+L RT +++AR  +         +KRRRFE
Sbjct: 1   MPFPFATPLLLFIAIMAFSISSLDEPDA-VRLLRTGIVKARSDIYN-------VKRRRFE 60

Query: 64  RRNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA 123
           R N V+AALG C  LYEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLEEVGA
Sbjct: 61  RGN-VQAALGDCHTLYEEADSRLAAMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGA 120

Query: 124 --AADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKAD 183
             AAD  NNLT LL GAL LY KIAAVEE    +R E+++  +N  I+LAKWN ATSK D
Sbjct: 121 VPAADG-NNLTMLLTGALHLYGKIAAVEELTTWERVEKERSRENWRINLAKWNAATSKPD 180

Query: 184 YVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGD 243
           YVVAMDGSGTH TIN AVAALAR GRRRRSERVVIYVKAGVY ENVEIGMQLKNV +VGD
Sbjct: 181 YVVAMDGSGTHTTINGAVAALARDGRRRRSERVVIYVKAGVYTENVEIGMQLKNVMVVGD 240

Query: 244 GADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLA 303
           GAD+T +TG+RNVPDGATTYSSATFGVSGDGFW RDITFENTAGPEK QAVALR+NSDLA
Sbjct: 241 GADKTIVTGNRNVPDGATTYSSATFGVSGDGFWVRDITFENTAGPEKQQAVALRLNSDLA 300

Query: 304 VVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANM 363
           VVYRCTIKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+IFVRRP+RHQANM
Sbjct: 301 VVYRCTIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQANM 360

Query: 364 ITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLI 423
           ITA GRDDPAE TGISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTVVM TDL+GLI
Sbjct: 361 ITAQGRDDPAEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVVMTTDLDGLI 420

Query: 424 DPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHG 483
           DP+GWGEW GDF +STLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV  FLHG
Sbjct: 421 DPKGWGEWRGDFAISTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAEFLHG 480

Query: 484 RDWIPVTGVPFQAGI 495
             WIP TGVPF+AG+
Sbjct: 481 DAWIPATGVPFEAGV 485

[Close]

BLAST of MC05g0275 vs. NCBI nr
Match: XP_022974141.1 (probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita maxima])

HSP 1 Score: 711 bits (1834), Expect = 2.56e-253
Identity = 366/494 (74.09%), Postives = 412/494 (83.40%), Query Frame = 0


Query: 4   MPSPFAAFLLLLLAV-ASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFE 63
           MP PFA  LLL LA+ ASSISS  +PD  V+L RT +++AR  +         +KRRRFE
Sbjct: 1   MPFPFATPLLLFLAIMASSISSLDEPDA-VRLLRTGIVKARSEIYN-------VKRRRFE 60

Query: 64  RRNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA 123
           R N V+ ALG C  LYEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLEEVGA
Sbjct: 61  RGN-VQTALGDCHTLYEEADSRLAGMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGA 120

Query: 124 AADRK-NNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADY 183
                 NNLT LL GAL LY+KIAAVEER   +R E+++L +N  I+LAKWN ATSK DY
Sbjct: 121 VPSADGNNLTMLLTGALHLYDKIAAVEERKCWERVEKERLRENGRINLAKWNAATSKPDY 180

Query: 184 VVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDG 243
           VVA DGSGT+ TIN AVAALAR GRRRRSERVVIYVKAGVY+ENVEIGMQLKNV +VGDG
Sbjct: 181 VVAKDGSGTYTTINGAVAALARDGRRRRSERVVIYVKAGVYKENVEIGMQLKNVMVVGDG 240

Query: 244 ADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAV 303
           AD+T +TG+RNVPDGATTYSSATFGVSGDGFWARDITFENTAGP K QAVALR+NSD  V
Sbjct: 241 ADKTIVTGNRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPGKQQAVALRLNSDRVV 300

Query: 304 VYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMI 363
           VYRCTIKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+IFVRRP+RHQANMI
Sbjct: 301 VYRCTIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQANMI 360

Query: 364 TAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLID 423
           TA GRDDPAE TGISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTV+M TDL+GLID
Sbjct: 361 TAQGRDDPAEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVIMTTDLDGLID 420

Query: 424 PRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGR 483
           P+GWGEW GDF LSTLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV GFL G 
Sbjct: 421 PKGWGEWRGDFALSTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAGFLRGD 480

Query: 484 DWIPVTGVPFQAGI 495
            WIP TGVPF+AG+
Sbjct: 481 AWIPATGVPFEAGV 485

[Close]

BLAST of MC05g0275 vs. NCBI nr
Match: KAG6597209.1 (Methionine aminopeptidase 1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 708 bits (1828), Expect = 2.41e-246
Identity = 362/478 (75.73%), Postives = 408/478 (85.36%), Query Frame = 0


Query: 18  VASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFERRNFVEAALGYCTRL 77
           +A SISS  +PD  V+L RT +++AR  +         +KRRRFER N V+AALG C  L
Sbjct: 1   MAFSISSLDEPDA-VRLLRTGIVKARSEIYN-------VKRRRFERGN-VQAALGDCHTL 60

Query: 78  YEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA--AADRKNNLTALLA 137
           YEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLE+VGA  AAD  NNLT LL 
Sbjct: 61  YEEADSRLAGMLVGENYSADDARIWLSAAVANHRSCLDGLEKVGAVPAADG-NNLTVLLT 120

Query: 138 GALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVVAMDGSGTHRTIN 197
           GAL LY+KIAAVEERN  +R E+++L +N  I+LAKWN ATSK DYVVAMDGSGTH TIN
Sbjct: 121 GALHLYDKIAAVEERNVRERVEKERLRENGRINLAKWNAATSKPDYVVAMDGSGTHTTIN 180

Query: 198 DAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGADRTTITGSRNVPD 257
            AVAALAR GRRRRSERVVIYVKAGVY+ENVEIGMQLKNV +VGDGAD+T +TG+RNVPD
Sbjct: 181 GAVAALARDGRRRRSERVVIYVKAGVYKENVEIGMQLKNVMVVGDGADKTIVTGNRNVPD 240

Query: 258 GATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLF 317
           GATTYSSATFGVSGDGFWARDITFENTAGPEK QAVALR+NSDLA+VYRCTIKGYQD+LF
Sbjct: 241 GATTYSSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAIVYRCTIKGYQDTLF 300

Query: 318 LHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMITAHGRDDPAENTGI 377
           LHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+IFVRRP+RHQANMITA GRDDPAE TGI
Sbjct: 301 LHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQANMITAQGRDDPAEATGI 360

Query: 378 SIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPRGWGEWEGDFPLS 437
           SI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTVVM TDL+GLIDP+GWGEW GDF +S
Sbjct: 361 SIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVVMTTDLDGLIDPKGWGEWRGDFAIS 420

Query: 438 TLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDWIPVTGVPFQA 493
           TLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV  FLHG  WIP TGVPF+A
Sbjct: 421 TLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAEFLHGDAWIPATGVPFEA 468

[Close]

BLAST of MC05g0275 vs. ExPASy TrEMBL
Match: A0A6J1CZF5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015995 PE=3 SV=1)

HSP 1 Score: 1026 bits (2652), Expect = 0.0
Identity = 514/519 (99.04%), Postives = 514/519 (99.04%), Query Frame = 0


Query: 4   MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFER 63
           MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFER
Sbjct: 1   MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFER 60

Query: 64  RNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA 123
           RNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA
Sbjct: 61  RNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA 120

Query: 124 ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV 183
           ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV
Sbjct: 121 ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV 180

Query: 184 AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD 243
           AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD
Sbjct: 181 AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD 240

Query: 244 RTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAVVY 303
           RTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAVVY
Sbjct: 241 RTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAVVY 300

Query: 304 RCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMITA 363
           RCTIKGYQD     SLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMITA
Sbjct: 301 RCTIKGYQD-----SLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMITA 360

Query: 364 HGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPR 423
           HGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPR
Sbjct: 361 HGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPR 420

Query: 424 GWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDW 483
           GWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDW
Sbjct: 421 GWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDW 480

Query: 484 IPVTGVPFQAGIRALGPLLENIYIYTWNLNSDKNHNERD 522
           IPVTGVPFQAGIRALGPLLENIYIYTWNLNSDKNHNERD
Sbjct: 481 IPVTGVPFQAGIRALGPLLENIYIYTWNLNSDKNHNERD 514

[Close]

BLAST of MC05g0275 vs. ExPASy TrEMBL
Match: A0A6J1G3Y9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450531 PE=3 SV=1)

HSP 1 Score: 712 bits (1837), Expect = 4.34e-254
Identity = 370/495 (74.75%), Postives = 414/495 (83.64%), Query Frame = 0


Query: 4   MPSPFAAFLLLLLAV-ASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFE 63
           MP PFA  LLL +A+ A SISS  +PD  V+L RT +++AR  +         +KRRRFE
Sbjct: 1   MPFPFATPLLLFIAIMAFSISSLDEPDA-VRLLRTGIVKARSDIYN-------VKRRRFE 60

Query: 64  RRNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA 123
           R N V+AALG C  LYEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLEEVGA
Sbjct: 61  RGN-VQAALGDCHTLYEEADSRLAAMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGA 120

Query: 124 --AADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKAD 183
             AAD  NNLT LL GAL LY KIAAVEE    +R E+++  +N  I+LAKWN ATSK D
Sbjct: 121 VPAADG-NNLTMLLTGALHLYGKIAAVEELTTWERVEKERSRENWRINLAKWNAATSKPD 180

Query: 184 YVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGD 243
           YVVAMDGSGTH TIN AVAALAR GRRRRSERVVIYVKAGVY ENVEIGMQLKNV +VGD
Sbjct: 181 YVVAMDGSGTHTTINGAVAALARDGRRRRSERVVIYVKAGVYTENVEIGMQLKNVMVVGD 240

Query: 244 GADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLA 303
           GAD+T +TG+RNVPDGATTYSSATFGVSGDGFW RDITFENTAGPEK QAVALR+NSDLA
Sbjct: 241 GADKTIVTGNRNVPDGATTYSSATFGVSGDGFWVRDITFENTAGPEKQQAVALRLNSDLA 300

Query: 304 VVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANM 363
           VVYRCTIKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+IFVRRP+RHQANM
Sbjct: 301 VVYRCTIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQANM 360

Query: 364 ITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLI 423
           ITA GRDDPAE TGISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTVVM TDL+GLI
Sbjct: 361 ITAQGRDDPAEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVVMTTDLDGLI 420

Query: 424 DPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHG 483
           DP+GWGEW GDF +STLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV  FLHG
Sbjct: 421 DPKGWGEWRGDFAISTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAEFLHG 480

Query: 484 RDWIPVTGVPFQAGI 495
             WIP TGVPF+AG+
Sbjct: 481 DAWIPATGVPFEAGV 485

[Close]

BLAST of MC05g0275 vs. ExPASy TrEMBL
Match: A0A6J1IAJ0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472734 PE=3 SV=1)

HSP 1 Score: 711 bits (1834), Expect = 1.24e-253
Identity = 366/494 (74.09%), Postives = 412/494 (83.40%), Query Frame = 0


Query: 4   MPSPFAAFLLLLLAV-ASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFE 63
           MP PFA  LLL LA+ ASSISS  +PD  V+L RT +++AR  +         +KRRRFE
Sbjct: 1   MPFPFATPLLLFLAIMASSISSLDEPDA-VRLLRTGIVKARSEIYN-------VKRRRFE 60

Query: 64  RRNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA 123
           R N V+ ALG C  LYEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLEEVGA
Sbjct: 61  RGN-VQTALGDCHTLYEEADSRLAGMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGA 120

Query: 124 AADRK-NNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADY 183
                 NNLT LL GAL LY+KIAAVEER   +R E+++L +N  I+LAKWN ATSK DY
Sbjct: 121 VPSADGNNLTMLLTGALHLYDKIAAVEERKCWERVEKERLRENGRINLAKWNAATSKPDY 180

Query: 184 VVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDG 243
           VVA DGSGT+ TIN AVAALAR GRRRRSERVVIYVKAGVY+ENVEIGMQLKNV +VGDG
Sbjct: 181 VVAKDGSGTYTTINGAVAALARDGRRRRSERVVIYVKAGVYKENVEIGMQLKNVMVVGDG 240

Query: 244 ADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAV 303
           AD+T +TG+RNVPDGATTYSSATFGVSGDGFWARDITFENTAGP K QAVALR+NSD  V
Sbjct: 241 ADKTIVTGNRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPGKQQAVALRLNSDRVV 300

Query: 304 VYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMI 363
           VYRCTIKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+IFVRRP+RHQANMI
Sbjct: 301 VYRCTIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQANMI 360

Query: 364 TAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLID 423
           TA GRDDPAE TGISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTV+M TDL+GLID
Sbjct: 361 TAQGRDDPAEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVIMTTDLDGLID 420

Query: 424 PRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGR 483
           P+GWGEW GDF LSTLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV GFL G 
Sbjct: 421 PKGWGEWRGDFALSTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAGFLRGD 480

Query: 484 DWIPVTGVPFQAGI 495
            WIP TGVPF+AG+
Sbjct: 481 AWIPATGVPFEAGV 485

[Close]

BLAST of MC05g0275 vs. ExPASy TrEMBL
Match: A0A5D3D3J9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003760 PE=3 SV=1)

HSP 1 Score: 684 bits (1765), Expect = 4.62e-243
Identity = 353/497 (71.03%), Postives = 402/497 (80.89%), Query Frame = 0


Query: 4   MPSPFAAFLLLLLAVASSISSFHDPDRE-VQLARTIVMEARDSVRGSVTGTGELKRRRFE 63
           MP PF  FLLLL     + S+F  P  + V+LA+T V EA       VT  G LKRRRF 
Sbjct: 1   MPFPFTTFLLLLCLPFVASSNFSLPALDSVRLAQTGVREA-------VTEIGNLKRRRFG 60

Query: 64  RRNFVEAALGYCTRLYEEADARLAELLFGGN--YTAEDARTWISAAVANHRSCTDGLEEV 123
             N + AAL  C +LYEEA+ RLA +L GG   Y+AED R W+SAA+ANHRSC DGLEEV
Sbjct: 61  DGNHLLAALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEV 120

Query: 124 GA--AADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSK 183
            A  AA   +NLT +L GAL LY+KIAAVE+RNG KR E++K  +NRGI+LA WNPATSK
Sbjct: 121 DADGAAVGGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSK 180

Query: 184 ADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIV 243
           ADYVVA DGSGTH TIN AVAALA +GRRR   R+VIYVKAGVYRENVEIG+QLKNV +V
Sbjct: 181 ADYVVAKDGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLV 240

Query: 244 GDGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSD 303
           GDG D+T +TGSRNVPDGATTY+SATFGVSGDGFWARDITFENTAGPEK QAVALR+NSD
Sbjct: 241 GDGIDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSD 300

Query: 304 LAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQA 363
           LAVVYRC IKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGNSAAVLQNCEIFVR+P RHQA
Sbjct: 301 LAVVYRCAIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQA 360

Query: 364 NMITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEG 423
           NMITA GRDDPAE TGISI  SRV+  P+FA V+ ++RT+LGRPWKR+SRTV+M TDL+G
Sbjct: 361 NMITAQGRDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVIMGTDLDG 420

Query: 424 LIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFL 483
           LIDP+GWGEW G+F +STLFY E+KN G GSSTRFRVNWPGFH+ R   EA  FTV  FL
Sbjct: 421 LIDPKGWGEWTGNFAISTLFYAEFKNRGDGSSTRFRVNWPGFHVLRSAEEARPFTVAEFL 480

Query: 484 HGRDWIPVTGVPFQAGI 495
           HG DWIP +GV F+AG+
Sbjct: 481 HGGDWIPASGVSFEAGV 490

[Close]

BLAST of MC05g0275 vs. ExPASy TrEMBL
Match: A0A1S3AVG1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483369 PE=3 SV=1)

HSP 1 Score: 684 bits (1765), Expect = 4.62e-243
Identity = 354/497 (71.23%), Postives = 402/497 (80.89%), Query Frame = 0


Query: 4   MPSPFAAFLLLLLAVASSISSFHDPDRE-VQLARTIVMEARDSVRGSVTGTGELKRRRFE 63
           MP PF  FLLLL     + S+F  P  + V+LA+T V EA       VT  G LKRRRF 
Sbjct: 1   MPFPFTTFLLLLCLPFVASSNFSLPALDSVRLAQTGVREA-------VTEIGNLKRRRFG 60

Query: 64  RRNFVEAALGYCTRLYEEADARLAELLFGGN--YTAEDARTWISAAVANHRSCTDGLEEV 123
             N + AAL  C +LYEEA+ RLA +L GG   Y+AED R W+SAA+ANHRSC DGLEEV
Sbjct: 61  DGNHLLAALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEV 120

Query: 124 GA--AADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSK 183
            A  AA   +NLT +L GAL LY+KIAAVE+RNG KR E++K  +NRGI+LA WNPATSK
Sbjct: 121 DADGAAVGGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSK 180

Query: 184 ADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIV 243
           ADYVVA DGSGTH TIN AVAALA +GRRR   R+VIYVKAGVYRENVEIG+QLKNV +V
Sbjct: 181 ADYVVAKDGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLV 240

Query: 244 GDGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSD 303
           GDG D+T +TGSRNVPDGATTY+SATFGVSGDGFWARDITFENTAGPEK QAVALR+NSD
Sbjct: 241 GDGIDKTIVTGSRNVPDGATTYNSATFGVSGDGFWARDITFENTAGPEKQQAVALRLNSD 300

Query: 304 LAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQA 363
           LAVVYRC IKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGNSAAVLQNCEIFVR+P RHQA
Sbjct: 301 LAVVYRCAIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQA 360

Query: 364 NMITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEG 423
           NMITA GRDDPAE TGISI  SRV+  P+FA V+ ++RT+LGRPWKR+SRTVVM TDL+G
Sbjct: 361 NMITAQGRDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVVMGTDLDG 420

Query: 424 LIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFL 483
           LIDP+GWGEW G+F +STLFY E+KN G GSSTRFRVNWPGFH+ R   EA  FTV  FL
Sbjct: 421 LIDPKGWGEWTGNFAISTLFYAEFKNRGDGSSTRFRVNWPGFHVLRSADEARPFTVAEFL 480

Query: 484 HGRDWIPVTGVPFQAGI 495
           HG DWIP +GV F+AG+
Sbjct: 481 HGGDWIPASGVSFEAGV 490

[Close]

BLAST of MC05g0275 vs. TAIR 10
Match: AT3G60730.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 545.8 bits (1405), Expect = 3.7e-155
Identity = 276/512 (53.91%), Postives = 358/512 (69.92%), Query Frame = 0


Query: 12  LLLLLAVASSISSFHDPDREV-QLARTIVMEARDSVRGSVTGTGELKRRRFERRNFVEAA 71
           ++  LA+A+ I++ +  + +V ++ART V+EA+ S  GS+  T           ++ +  
Sbjct: 13  IMFFLAIAAVITASNTAELDVLEMARTAVVEAKTSF-GSMAVTEATSE---VAGSYYKLG 72

Query: 72  LGYCTRLYEEADARLAELLFG-GNYTAEDARTWISAAVANHRSCTDGL--EEVGAAADRK 131
           L  C +LY+E++ARL++L+    N+T ED RTW+S  +ANH +C DGL  +  G      
Sbjct: 73  LSECEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKPLVH 132

Query: 132 NNLTALLAGALRLYEKI---------AAVEERNGLKRWEEQKLTKNRGID---------- 191
           +N+T +L  AL  Y+K              + +G  R + +    N G            
Sbjct: 133 SNVTFVLHEALAFYKKSRGHMKKRLHGPARQGHGPTRPKHRPTRPNHGPGRSHHGPSRPN 192

Query: 192 -----LAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYR 251
                L  WNP +S+AD+VVA DGS THRTIN A+AA++R G+ R + RV+IY+KAGVY 
Sbjct: 193 QNGGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGKSRLN-RVIIYIKAGVYN 252

Query: 252 ENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTA 311
           E +EI   +KN+ +VGDG DRT +T +RNVPDG+TTY SATFGVSGDGFWARDITFENTA
Sbjct: 253 EKIEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITFENTA 312

Query: 312 GPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVL 371
           GP KHQAVALRV+SDL++ YRC+ KGYQD+LF HSLRQFYRDC IYGT+DFIFG++AAV 
Sbjct: 313 GPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDAAAVF 372

Query: 372 QNCEIFVRRPMRHQANMITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPW 431
           QNC+IFVRRPM HQ NMITA GRDDP  N+GISI  SR+RAAPEF AV+G F+++LGRPW
Sbjct: 373 QNCDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYLGRPW 432

Query: 432 KRYSRTVVMATDLEGLIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIF 491
           K+YSRTV + TD++ LIDPRGW EW G + LSTL+YGE+ NTG G+ T  RVNWPGFH+ 
Sbjct: 433 KKYSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPGFHVL 492

Query: 492 RGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 496
           RG+ EA  FTV  F+ G  WIP+TGVPF AG+
Sbjct: 493 RGEEEASPFTVSRFIQGDSWIPITGVPFSAGV 519

[Close]

BLAST of MC05g0275 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 386.3 bits (991), Expect = 3.8e-107
Identity = 202/453 (44.59%), Postives = 272/453 (60.04%), Query Frame = 0


Query: 67  VEAALGYCTRLYEEADARLAELL-----FGGNYTAEDARTWISAAVANHRSCTDGLEE-- 126
           V +A   C  L +++   L   L       G+ +  D  TW+S+A+ NH +CTDG +E  
Sbjct: 137 VRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEIE 196

Query: 127 ----------VGAAADRK---NNLTALLAGALRLYEKIAAVEERNGLKRWEEQKL---TK 186
                     +GA  D     +N  A+ AG ++    +  V  R  L   E ++L    K
Sbjct: 197 GQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEELPNWLK 256

Query: 187 NRGIDLAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVY- 246
               +L     +  +AD  V+ DGSGT +TI +A+    +      S R VIYVKAG Y 
Sbjct: 257 REDRELLGTPTSAIQADITVSKDGSGTFKTIAEAI----KKAPEHSSRRFVIYVKAGRYE 316

Query: 247 RENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENT 306
            EN+++G +  N+  +GDG  +T ITG +++ D  TT+ +ATF  +G GF  RD+TFEN 
Sbjct: 317 EENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENY 376

Query: 307 AGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAV 366
           AGP KHQAVALRV  D AVVYRC I GYQD+L++HS RQF+R+C IYGTVDFIFGN+A +
Sbjct: 377 AGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVI 436

Query: 367 LQNCEIFVRRPMRHQANMITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRP 426
           LQ+C I+ R+PM  Q   ITA  R DP +NTGISIH  ++ A P+  A +G + T+LGRP
Sbjct: 437 LQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRP 496

Query: 427 WKRYSRTVVMATDLEGLIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHI 486
           WK YSR V M +D+   IDPRGW EW G F L +L+YGEY N G GS    RV WPG+H+
Sbjct: 497 WKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHV 556

Query: 487 FRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 496
               +EA +FTV  F+ G  W+P TGV F +G+
Sbjct: 557 ITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

[Close]

BLAST of MC05g0275 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 385.6 bits (989), Expect = 6.5e-107
Identity = 214/436 (49.08%), Postives = 276/436 (63.30%), Query Frame = 0


Query: 68  EAALGYCTRLYEEADARLAELLFGGNYTAE-DARTWISAAVANHRSCTDGLEEVGAA--- 127
           +AA   C +LY+   +++ E +      ++ DA+TW+S A+ N  +C  G  E+G     
Sbjct: 94  KAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIV 153

Query: 128 -ADRKNNLTALLAGALRLYEKIA---AVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKA 187
                NN++ LL   L +  K+       E++G   W +         D      +T K 
Sbjct: 154 LPLMSNNVSNLLCNTLAI-NKVPFNYTPPEKDGFPSWVKPG-------DRKLLQSSTPKD 213

Query: 188 DYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVG 247
           + VVA DGSG  +TI +A+ A + SG      R VIYVK GVY EN+EI  + KNV + G
Sbjct: 214 NAVVAKDGSGNFKTIKEAIDAASGSG------RFVIYVKQGVYSENLEI--RKKNVMLRG 273

Query: 248 DGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPEKHQAVALRVNSDL 307
           DG  +T ITGS++V  G TT++SAT    GDGF AR ITF NTAG    QAVALR  SDL
Sbjct: 274 DGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDL 333

Query: 308 AVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQAN 367
           +V Y+C+ + YQD+L++HS RQFYRDC +YGTVDFIFGN+AAVLQNC IF RRP R + N
Sbjct: 334 SVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTN 393

Query: 368 MITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGL 427
            ITA GR DP +NTGI IH SRV AA +   V G  +T+LGRPW++YSRTV M T L+ L
Sbjct: 394 TITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSL 453

Query: 428 IDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLH 487
           IDPRGW EW+G+F L TLFY E++NTG G+ST  RV WPGF +     EA +FTVG FL 
Sbjct: 454 IDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLA 511

Query: 488 GRDWIPVTGVPFQAGI 496
           G  WIP + VPF +G+
Sbjct: 514 GGSWIP-SSVPFTSGL 511

[Close]

BLAST of MC05g0275 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 382.1 bits (980), Expect = 7.1e-106
Identity = 214/464 (46.12%), Postives = 277/464 (59.70%), Query Frame = 0


Query: 69  AALGYCTRLYEEADARL--AELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAAADR 128
           +AL  C  LYE+   +L  +   +G   +  D +T +SAA+AN  +C +G  +    +  
Sbjct: 102 SALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDFKLTSSY 161

Query: 129 K--------NNLTALLAGALRLYEKIA---AVEER---NGLKRWEEQKLTKNRGI----- 188
                     NLT  ++ +L + +  A   AV E+    G  ++ +Q+ +   G      
Sbjct: 162 SKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLL 221

Query: 189 -----DLAKWNP-----------ATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSE 248
                    W P            T+KAD VVA DGSG + +I  AV A A+    RR++
Sbjct: 222 LFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAK--LPRRNQ 281

Query: 249 RVVIYVKAGVYRENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFGVSGDG 308
           R+VIYVKAGVYRENV I   +KNV ++GDG D T +TG+RNV DG TT+ SATF VSG+G
Sbjct: 282 RLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFAVSGNG 341

Query: 309 FWARDITFENTAGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGT 368
           F A+ ITFENTAGPEKHQAVALR +SD +V Y C+ KGYQD+L+LHS RQF R+C IYGT
Sbjct: 342 FIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNIYGT 401

Query: 369 VDFIFGNSAAVLQNCEIFVRRPMRHQANMITAHGRDDPAENTGISIHGSRVRAAPEFAAV 428
           VDFIFG++ A+LQNC I+ R+PM  Q N ITA  R +P E TG  I  S V  A E    
Sbjct: 402 VDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE---- 461

Query: 429 QGEFRTFLGRPWKRYSRTVVMATDLEGLIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSST 488
                T+LGRPW+ +SRTV M  +L  L+ P GW  W G F LSTL+YGEY NTG G+S 
Sbjct: 462 -----TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASV 521

Query: 489 RFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 496
             RV WPG+H+ +   EA +FTV  FL G  WI  TGVP   G+
Sbjct: 522 SGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

[Close]

BLAST of MC05g0275 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 377.1 bits (967), Expect = 2.3e-104
Identity = 199/449 (44.32%), Postives = 282/449 (62.81%), Query Frame = 0


Query: 69  AALGYCTRLYE---EADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEV--GAA 128
           +A   C  L +   +A +R    +   +   +D  TW+SAA+ NH +CT+G + V  G  
Sbjct: 152 SAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALTNHDTCTEGFDGVDDGGV 211

Query: 129 ADRK----NNLTALLAGALRLY------EKIAAVEERN----GLKRWEEQ--KLTKNRGI 188
            D       NL+ L++  L ++      +  A V  +N    G++  EE+  +  + +  
Sbjct: 212 KDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEKFPRWMRPKER 271

Query: 189 DLAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRE-NV 248
           ++ +   +  +AD +V+ DG+GT +TI++A+    ++  R    R++IYVKAG Y E N+
Sbjct: 272 EILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTR----RIIIYVKAGRYEENNL 331

Query: 249 EIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFGVSGDGFWARDITFENTAGPE 308
           ++G +  N+  VGDG  +T I+G +++ D  TT+ +A+F  +G GF ARDITFEN AGP 
Sbjct: 332 KVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPA 391

Query: 309 KHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNC 368
           KHQAVALR+ +D AV+YRC I GYQD+L++HS RQF+R+C IYGTVDFIFGN+A VLQNC
Sbjct: 392 KHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNC 451

Query: 369 EIFVRRPMRHQANMITAHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRY 428
            I+ R+PM  Q N ITA  R DP +NTGISIH SRV AA +  A  G  +T+LGRPWK +
Sbjct: 452 SIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLF 511

Query: 429 SRTVVMATDLEGLIDPRGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGD 488
           SRTV M + + G +  RGW EW   F L TL+YGEY N+G GS    RV+WPG+ +    
Sbjct: 512 SRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINST 571

Query: 489 LEAGRFTVGGFLHGRDWIPVTGVPFQAGI 496
            EA RFTV  F++G  W+P TGV F AG+
Sbjct: 572 AEANRFTVAEFIYGSSWLPSTGVSFLAGL 596

The following BLAST results are available for this feature:

BLAST of MC05g0275 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84R10	5.3e-154	53.91	Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana OX=3...	[more]
O04887	1.0e-109	45.45	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
Q9FK05	5.3e-106	44.59	Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3...	[more]
O22149	9.1e-106	49.08	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
O49298	1.0e-104	46.12	Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of MC05g0275 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146909.1	0.0	99.04	pectinesterase [Momordica charantia]	[more]
XP_023540284.1	4.04e-257	75.56	probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita pepo subsp. pepo]	[more]
XP_022946489.1	8.96e-254	74.75	probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita moschata]	[more]
XP_022974141.1	2.56e-253	74.09	probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita maxima]	[more]
KAG6597209.1	2.41e-246	75.73	Methionine aminopeptidase 1A, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MC05g0275 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CZF5	0.0	99.04	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015995 PE=3 SV=1	[more]
A0A6J1G3Y9	4.34e-254	74.75	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450531 PE=3 SV=1	[more]
A0A6J1IAJ0	1.24e-253	74.09	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472734 PE=3 SV=1	[more]
A0A5D3D3J9	4.62e-243	71.03	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00376...	[more]
A0A1S3AVG1	4.62e-243	71.23	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483369 PE=3 SV=1	[more]

BLAST of MC05g0275 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G60730.1	3.7e-155	53.91	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G53370.1	3.8e-107	44.59	pectin methylesterase PCR fragment F	[more]
AT2G45220.1	6.5e-107	49.08	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G23200.1	7.1e-106	46.12	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G49220.1	2.3e-104	44.32	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 21..134 e-value: 1.1E-8 score: 35.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 174..495 e-value: 9.0E-113 score: 378.8
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 180..480 e-value: 1.2E-133 score: 444.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 18..145 e-value: 4.6E-9 score: 38.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 63..132
None	No IPR available	PANTHER	PTHR31707:SF186	PECTINESTERASE/PECTINESTERASE INHIBITOR 36-RELATED	coord: 11..494
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 11..494
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 328..337
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 175..494

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0275.1	MC05g0275.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity