MC05g0262 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0262 vs. ExPASy Swiss-Prot
Match: F4IXW2 (Brefeldin A-inhibited guanine nucleotide-exchange protein 5 OS=Arabidopsis thaliana OX=3702 GN=BIG5 PE=1 SV=2)

HSP 1 Score: 2486.1 bits (6442), Expect = 0.0e+00
Identity = 1299/1782 (72.90%), Postives = 1488/1782 (83.50%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGF+ RAF++MLKE S GKK+P LQKAIQAY D +K   Q   S+  E+SQ   + G
Sbjct: 1    MAAGGFLTRAFDTMLKE-SGGKKFPDLQKAIQAYQDGSKVVTQAAPSSIVESSQ---AEG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
               +TG E DE + V  +AE A+   +  +  E I+V LANAGH L GA+ E+VL PLRL
Sbjct: 61   GGEKTGVEADEPQKV-TSAEVAQQASQ--SKSETINVSLANAGHTLGGAEVELVLKPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AF+TKNLK+ + +LDCLHKLIAYDHLEGDPGL+GGKN   FTDILNM+C CVDNSS DST
Sbjct: 121  AFETKNLKIFDAALDCLHKLIAYDHLEGDPGLDGGKNSAPFTDILNMVCSCVDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            +LQVLKVLLTAVAS KF+VHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISI+FR
Sbjct: 181  VLQVLKVLLTAVASGKFKVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIVFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETD VS S++   +E +S +TSS   EE    DEN+KE TLGDAL   KDT+LASVEE
Sbjct: 241  RMETDIVSASSTVSQEEHVSGDTSSPKNEEITAADENEKEMTLGDALTQAKDTTLASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            L  L GGADIKGLEA LDKAVH EDGKK+ RGI+LES++I +RDALLVFRT+CKMGMKED
Sbjct: 301  LHTLVGGADIKGLEAALDKAVHLEDGKKIKRGIELESMSIGQRDALLVFRTLCKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
            +DEVTTKTRILSLELLQG+LEGVS +FTK+FHFIDSVKAYLSYALLRASVSQS VIFQYA
Sbjct: 361  SDEVTTKTRILSLELLQGMLEGVSHSFTKNFHFIDSVKAYLSYALLRASVSQSSVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            +GIFSVLLLRFR+SLKGEIGIFFP+IVLRSLD ++ P +QK+ VL+MLEK+C++PQ+LVD
Sbjct: 421  SGIFSVLLLRFRDSLKGEIGIFFPIIVLRSLDNSECPNDQKMGVLRMLEKVCKDPQMLVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            ++VNYDCDLEAPNLFERMVTTLSKI+QG+Q+ADPN    SQT S+KGSSLQCLVNVLKSL
Sbjct: 481  VYVNYDCDLEAPNLFERMVTTLSKIAQGSQSADPNPAMASQTASVKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEK R +++  + N    E+ +S  E +E KSRED+ SNFEKAKAHKST+EAAISEFN
Sbjct: 541  VDWEKIRREAENSTRNAN--EDSASTGEPIETKSREDVPSNFEKAKAHKSTMEAAISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            R  VKGVEYLI+NKLVE  P+SVAQFLR+T SL K MIGDYLGQHEEFP+AVMHAYVDSM
Sbjct: 601  RNSVKGVEYLIANKLVERNPASVAQFLRSTSSLSKVMIGDYLGQHEEFPLAVMHAYVDSM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            +FS MKF +AIR+FLKGFRLPGEAQKIDRIMEKFAERYCA NPGLFKNADTAYVLAYAVI
Sbjct: 661  KFSEMKFHSAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDR- 780
            MLNTDAHNPMVWPKMS+ DF RMN TNDPE+CAPTELLE+IYDSIV+EEIK+KDD + + 
Sbjct: 721  MLNTDAHNPMVWPKMSKSDFTRMNATNDPEDCAPTELLEEIYDSIVQEEIKLKDDDTMKK 780

Query: 781  -GKNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQ 840
                R  GEERGGLVSILNL LP+R S+ DAKSE+E IV++TQ IFR  G KRG+F+T +
Sbjct: 781  LSSQRPGGEERGGLVSILNLGLPKRISAADAKSETEDIVRKTQEIFRKHGVKRGVFHTVE 840

Query: 841  RIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFL 900
            +++++RPMVEAVGWPLLA FSVTME GDNKPR++LCMEGF+AGIHI +VLGMDTMRYAFL
Sbjct: 841  QVDIIRPMVEAVGWPLLAAFSVTMEVGDNKPRILLCMEGFKAGIHIAYVLGMDTMRYAFL 900

Query: 901  TSLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSI 960
            TSLVRFTFLHAPKEMRSKNVEALR LL LCD E ++LQDTWNAVLECVSRLEFI STP I
Sbjct: 901  TSLVRFTFLHAPKEMRSKNVEALRILLGLCDSEPDTLQDTWNAVLECVSRLEFIISTPGI 960

Query: 961  ASTVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQ 1020
            A+TVM+GSNQISRD VVQSLKEL+G+PA+QVFVNSVKLPS+SVVEFFTALCGVSAEELKQ
Sbjct: 961  AATVMHGSNQISRDGVVQSLKELAGRPAEQVFVNSVKLPSESVVEFFTALCGVSAEELKQ 1020

Query: 1021 TPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLG 1080
            +PARVFSLQKLVEISYYN+ARIRMVWARIWSVL+ HF+SAGSHHDEKIAMYAIDSLRQLG
Sbjct: 1021 SPARVFSLQKLVEISYYNIARIRMVWARIWSVLAEHFVSAGSHHDEKIAMYAIDSLRQLG 1080

Query: 1081 MKYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSV 1140
            MKYLERAEL NFTFQNDILKPFV++MRN+ S++IRSLIVDCIVQMIKSKVG+IKSGWRSV
Sbjct: 1081 MKYLERAELTNFTFQNDILKPFVIIMRNTQSQTIRSLIVDCIVQMIKSKVGSIKSGWRSV 1140

Query: 1141 FMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISL 1200
            FMIFTA+ADDE ESIVE +FENVEQVILEHFDQV+GDCFMDCVNCLIRFANNK+S RISL
Sbjct: 1141 FMIFTAAADDEVESIVEKSFENVEQVILEHFDQVIGDCFMDCVNCLIRFANNKASDRISL 1200

Query: 1201 KAIALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDLTSDSRPEVR 1260
            KAIALLRICEDRLAEGLIPGG LKP+  + D  FDVTEHYWFPMLAGLSDLTSD RPEVR
Sbjct: 1201 KAIALLRICEDRLAEGLIPGGVLKPVDGNEDETFDVTEHYWFPMLAGLSDLTSDYRPEVR 1260

Query: 1261 SCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSIH 1320
            +CALEVLFDLLNERG KFST FWESIFHR+LFPIFDH+   GKE+LI+SGD   RETSIH
Sbjct: 1261 NCALEVLFDLLNERGNKFSTPFWESIFHRILFPIFDHVSHAGKESLISSGDVKFRETSIH 1320

Query: 1321 SLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSED 1380
            SLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAK+ +Q+VVS++LGALVHLIEVGGHQFSE 
Sbjct: 1321 SLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKKSDQTVVSISLGALVHLIEVGGHQFSEG 1380

Query: 1381 DWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVGD 1440
            DWD LLKSIRDASYTTQPLELLNAL F+NP    V +   I   +        +  D+ D
Sbjct: 1381 DWDMLLKSIRDASYTTQPLELLNALSFDNPKKNLV-LAGDIEADASDSPRVDRNPDDIKD 1440

Query: 1441 HGKASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTIGQ 1500
            +GK S  ASPR    T     ESG+   +D  +EG PS S RA K  +  +LQRSQT GQ
Sbjct: 1441 NGKVSAQASPR--IGTHGTSLESGIPPKADG-SEGRPSSSGRAQKDVDDVNLQRSQTFGQ 1500

Query: 1501 RIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRGKC 1560
            R     MDN+F+R+LTS+ K   ++ +VPSSP K   D  +P+ +E EES  +  +RGKC
Sbjct: 1501 R----FMDNLFLRNLTSQPKSSVAEVTVPSSPYK-HEDPTEPDSRE-EESPALGAIRGKC 1560

Query: 1561 ITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPDER 1620
            ITQLLLLG I+ IQ+KYW  L  PQKIAIMDIL S +EFA++YNSY+NLR RM+HIP ER
Sbjct: 1561 ITQLLLLGAINSIQQKYWSNLKTPQKIAIMDILFSFIEFASSYNSYSNLRTRMNHIPTER 1620

Query: 1621 PPLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDSSA 1680
            PPLNLLRQEL GT++YLD+L K TS                            +   S++
Sbjct: 1621 PPLNLLRQELEGTTIYLDVLQKTTSGL--------------------------ADDASNS 1680

Query: 1681 AKNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCFMN 1740
               ++G AE +LVSFCEQ L+E SDL S+ GE T+MDVHRVLELRSPVIVKV++GMCFMN
Sbjct: 1681 EDRLEGAAEEKLVSFCEQVLKETSDLQSTLGETTNMDVHRVLELRSPVIVKVLEGMCFMN 1737

Query: 1741 SEIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1781
            + IFR+H+REFYPLLT+LVCC+Q+++RGAL +LFK QLK LL
Sbjct: 1741 NTIFRKHMREFYPLLTRLVCCEQMEIRGALANLFKAQLKPLL 1737

BLAST of MC05g0262 vs. ExPASy Swiss-Prot
Match: Q9LPC5 (Brefeldin A-inhibited guanine nucleotide-exchange protein 3 OS=Arabidopsis thaliana OX=3702 GN=BIG3 PE=1 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 2.7e-258
Identity = 556/1433 (38.80%), Postives = 809/1433 (56.45%), Query Frame = 0

Query: 109  ADAEIVLNPLRLAFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMI 168
            A++EI+L+PL  A  T  LK+++ ++DC+ KLIA+ ++ G+    GG    + + ++  I
Sbjct: 77   AESEIILSPLINASSTGVLKIVDPAVDCIQKLIAHGYVRGEADPTGGPEALLLSKLIETI 136

Query: 169  CGCVDNSSSDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSK 228
            C C +       +L VLK LLTAV S   R+HG+ LL ++R CY I L S++ +NQAT+K
Sbjct: 137  CKCHELDDEGLELL-VLKTLLTAVTSISLRIHGDSLLQIVRTCYGIYLGSRNVVNQATAK 196

Query: 229  AMLTQMISIIFRRMETDQVSL-----------------STSSGTKESISAETSSKVVEET 288
            A L QM  I+FRRME D  ++                  +   T +S+    +  + +  
Sbjct: 197  ASLVQMSVIVFRRMEADSSTVPIQPIVVAELMEPMDKSESDPSTTQSVQGFITKIMQDID 256

Query: 289  AVTDENDKETTLGDALNSVKDTSLASVEELQNLAGGADIKGLEA---------------- 348
             V +  + + T G   +   +TSL       +L    D   L+A                
Sbjct: 257  GVFNSANAKGTFG-GHDGAFETSLPGTANPTDLLDSTDKDMLDAKYWEISMYKSALEGRK 316

Query: 349  --VLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMK--EDADEVTTKTRIL 408
              + D  V  +D  ++  G      N ++RDA LVFR +CK+ MK     D    + +I+
Sbjct: 317  GELADGEVEKDDDSEVQIG------NKLRRDAFLVFRALCKLSMKTPPKEDPELMRGKIV 376

Query: 409  SLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYATGIFSVLLLRF 468
            +LELL+ LLE     F     F+ ++K YL  +LL+ S S   +IFQ +  I   L+ RF
Sbjct: 377  ALELLKILLENAGAVFRTSDRFLGAIKQYLCLSLLKNSASNLMIIFQLSCSILLSLVSRF 436

Query: 469  RESLKGEIGIFFPLIVLRSLDGTDFP-VNQKISVLKMLEKICREPQILVDIFVNYDCDLE 528
            R  LK EIG+FFP+IVLR L+    P   QK+ VL+ L+K+C + QILVDIF+NYDCD+ 
Sbjct: 437  RAGLKAEIGVFFPMIVLRVLENVAQPDFQQKMIVLRFLDKLCVDSQILVDIFINYDCDVN 496

Query: 529  APNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSLVDWEKSRLQ- 588
            + N+FERMV  L K +QG        +   Q  ++K  +++CLV VL+S+ DW   +L+ 
Sbjct: 497  SSNIFERMVNGLLKTAQGVPPGTVTTLLPPQEAAMKLEAMKCLVAVLRSMGDWVNKQLRL 556

Query: 589  ----SDKQSENV-RSLEEESSGYEN----------------LEIKSREDLTSNFEKAKAH 648
                S K  E V R+LEE S   EN                 E+ S        E+ +A+
Sbjct: 557  PDPYSAKMLEIVDRNLEEGSHPVENGKGDGGHGGFERSDSQSELSSGNSDALAIEQRRAY 616

Query: 649  KSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEF 708
            K  ++  IS FN+KP KG+E+LI    V ++P  +A FL++   L+KT+IGDYLG+ E+ 
Sbjct: 617  KLELQEGISIFNQKPKKGIEFLIKANKVGDSPEEIAAFLKDASGLNKTLIGDYLGEREDL 676

Query: 709  PVAVMHAYVDSMQFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKN 768
             + VMHAYVDS +F GM+FD AIR FL+GFRLPGEAQKIDRIMEKFAER+C  NP  F +
Sbjct: 677  SLKVMHAYVDSFEFQGMEFDEAIRAFLRGFRLPGEAQKIDRIMEKFAERFCKCNPKDFSS 736

Query: 769  ADTAYVLAYAVIMLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKE 828
            ADTAYVLAY+VI+LNTDAHNPMV  KM+   FIR N   D  +  P E L  +Y+ I + 
Sbjct: 737  ADTAYVLAYSVILLNTDAHNPMVKSKMTADGFIRNNRGIDDGKDLPEEYLRALYERISRN 796

Query: 829  EIKMKDD-TSDRGKNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQ 888
            EIKMKDD    + K         GL +ILN+ +PRR    + ++ S+ +++  Q  F+ +
Sbjct: 797  EIKMKDDGLGPQQKQPTNSSRLLGLDTILNIVVPRRGDDMNMET-SDDLIRHMQERFKEK 856

Query: 889  GGK-RGIFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITH 948
              K   ++Y +  + ++R MVE    P+LA FSV +++ D+     LC+EGF   IH+T 
Sbjct: 857  ARKSESVYYAASDVIILRFMVEVCWAPMLAAFSVPLDQSDDAVITTLCLEGFHHAIHVTS 916

Query: 949  VLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECV 1008
            V+ + T R AF+TSL +FT LH+P +++ KN+EA++ ++ L + E   LQD W  +L CV
Sbjct: 917  VMSLKTHRDAFVTSLAKFTSLHSPADIKQKNIEAIKAIVKLAEEEGNYLQDAWEHILTCV 976

Query: 1009 SRLEFI----TSTPSIASTVMY-----GSNQISRDAVVQSLKE----------------- 1068
            SR E +       P  A+   +     G++ +++   V ++KE                 
Sbjct: 977  SRFEHLHLLGEGAPPDATFFAFPQTESGNSPLAKPNSVPAIKERAPGKLQYAASAMIRGS 1036

Query: 1069 -----LSGKPAD------------------------QVFVNSVKLPSDSVVEFFTALCGV 1128
                 ++GK ++                        ++F  S +L S+++++F  ALC V
Sbjct: 1037 YDGSGVAGKASNTVTSEQMNNLISNLNLLEQVGDMSRIFTRSQRLNSEAIIDFVKALCKV 1096

Query: 1129 SAEELKQ-TPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYA 1188
            S +EL+  +  RVFSL K+VEI++YNM RIR+VW+ IW VLS+ F++ G   +  IA++A
Sbjct: 1097 SMDELRSPSDPRVFSLTKIVEIAHYNMNRIRLVWSSIWHVLSDFFVTIGCSDNLSIAIFA 1156

Query: 1189 IDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGN 1248
            +DSLRQL MK+LER ELAN+ FQN+ +KPFVV+MR S +  IR LI+ C+ QM+ S+V N
Sbjct: 1157 MDSLRQLSMKFLEREELANYNFQNEFMKPFVVVMRKSGAVEIRELIIRCVSQMVLSRVDN 1216

Query: 1249 IKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVV---GDCFMDCVNCLIRF 1308
            +KSGW+S+FMIFT +A D  ++IV  +FE VE++I ++F  +       F DCVNCL+ F
Sbjct: 1217 VKSGWKSMFMIFTTAAHDAHKNIVFLSFEMVEKIIRDYFPHITETETTTFTDCVNCLVAF 1276

Query: 1309 ANNKSSHRISLKAIALLRICEDRLAEG-----------LIPGGALKPIHDSADPAFDVTE 1368
             N K    ISL+AIA L+ C  +LAEG           L P G      DS     +  E
Sbjct: 1277 TNCKFEKDISLQAIAFLQYCARKLAEGYVGSSLRRNPPLSPQGGKIGKQDSG-KFLESDE 1336

Query: 1369 HY--WFPMLAGLSDLTSDSRPEVRSCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFD 1397
            H   WFP+LAGLS+L+ D R E+R  AL+VLFD L   G  FS + WE +F  VLF IFD
Sbjct: 1337 HLYSWFPLLAGLSELSFDPRAEIRKVALKVLFDTLRNHGDHFSLALWERVFESVLFRIFD 1396

BLAST of MC05g0262 vs. ExPASy Swiss-Prot
Match: Q9LZX8 (Brefeldin A-inhibited guanine nucleotide-exchange protein 2 OS=Arabidopsis thaliana OX=3702 GN=BIG2 PE=2 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.2e-250
Identity = 628/1916 (32.78%), Postives = 959/1916 (50.05%), Query Frame = 0

Query: 25   PALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAGDTSETGGEVDEARTVEETAEEAEN 84
            PAL+K +       K A+  +HS  A            +E    ++   +++++   + +
Sbjct: 16   PALEKIV-------KNASWRKHSKLA------------NECKAVIERLNSLQKSPPPSSS 75

Query: 85   VGRKAAPQEPISVVLANAGHV-LEGADAEIVLNPLRLAFDTKNLKVIELSLDCLHKLIAY 144
                +  +  +   L + G +    AD+E++ +PL  A  T   K+IE ++DC+ KLIA+
Sbjct: 76   AATDSESESSVPGPLNDGGSIEYSLADSELIFSPLINACGTGLAKIIEPAIDCIQKLIAH 135

Query: 145  DHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDSTILQVLKVLLTAVASAKFRVHGEP 204
             ++ G+    GG    +   +++ +C C D    +S  L VLK LL+A+ S   R+HG+ 
Sbjct: 136  GYIRGESDPSGGAESLLLFKLIDSVCKCHD-LGDESIELPVLKTLLSAINSISLRIHGKC 195

Query: 205  LLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFRRMETDQVSL-------------- 264
            LL V+R CY+I L SK+ +NQ T+KA L Q++ I+FRRME D  ++              
Sbjct: 196  LLLVVRTCYDIYLGSKNVVNQTTAKASLIQILVIVFRRMEADSSTVPIQPIVVAELMEPL 255

Query: 265  --STSSGTKESISAETSSKVVEET------------AVTDENDKETTLG-DALNSVKDTS 324
              S + GT         +K++++             + +    ++   G   + +   T 
Sbjct: 256  EKSDADGTMTQFVQGFITKIMQDIDGVLNPTMSGSGSGSGSGGQDGAYGTTTVETTNPTD 315

Query: 325  LASVEELQNL-AGGADIKGLEAVLD--KAVHTEDGKKMSRGIDLESVNIVKRDALLVFRT 384
            L    +   L A   +I   ++ L+  K   T+   +    ++++  N ++RDA LVFR 
Sbjct: 316  LLDSTDKDMLDAKYWEISMYKSALEGRKGELTDGDAERDDDLEVQIENKLRRDACLVFRA 375

Query: 385  ICKMGMK-----EDADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALL 444
            +CK+ MK       AD  + + +IL+LELL+ LLE     F     F   +K +L  +LL
Sbjct: 376  LCKLSMKAPPKESSADPQSMRGKILALELLKILLENAGAVFRTSEKFSADIKQFLCLSLL 435

Query: 445  RASVSQSPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFP-VNQKISVL 504
            + S S   +IFQ +  IF  L+ RFR  LK EIG+FFP+IVLR ++    P   QK+ VL
Sbjct: 436  KNSASTLMIIFQLSCSIFISLVARFRAGLKAEIGVFFPMIVLRVVENVAQPNFQQKMIVL 495

Query: 505  KMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSI 564
            + L+K+C + QILVDIF+NYDCD+ + N+FERMV  L K +QG        +   Q  ++
Sbjct: 496  RFLDKLCLDSQILVDIFLNYDCDVNSSNIFERMVNGLLKTAQGVPPGTATTLMPPQEAAM 555

Query: 565  KGSSLQCLVNVLKSLVDWEKSRLQ-----SDKQSENVR-------------SLEEESSGY 624
            K  +++CLV +LKS+ DW   +L+     S  +S+ +              + +E + G 
Sbjct: 556  KLEAMKCLVAILKSMGDWLNKQLRLPVSNSLNKSDVIEIDLGPGSPQLANGNADESADGS 615

Query: 625  ENL-EIKSREDLTSNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVAQF 684
            +   E           E+ +A+K  ++  IS FNRKP KG+E+LI+   V  +P  +A F
Sbjct: 616  DTYSESSGGTSDALAIEQRRAYKLELQEGISLFNRKPTKGIEFLINAGKVGESPEEIAGF 675

Query: 685  LRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMQFSGMKFDAAIRDFLKGFRLPGEAQK 744
            L++   L+KT+IGDYLG+ E+  + VMHAYVDS  F GM+FD AIR FL+GFRLPGEAQK
Sbjct: 676  LKDASGLNKTLIGDYLGEREDLALKVMHAYVDSFDFRGMEFDEAIRTFLEGFRLPGEAQK 735

Query: 745  IDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSRPDFIRMNVT 804
            IDRIMEKFAERYC  NP +F +AD+AYVLAY+VIMLNTDAHNPMV  KMS  DFIR N  
Sbjct: 736  IDRIMEKFAERYCKCNPKVFTSADSAYVLAYSVIMLNTDAHNPMVKNKMSADDFIRNNRG 795

Query: 805  NDPEECAPTELLEDIYDSIVKEEIKMK-DDTSDRGKNRREGEERGGLVSILNLALPRRQS 864
             D  +  P + +  +Y+ I K EIKMK DD   + K         GL  ILN+ + R+Q 
Sbjct: 796  IDDGKDLPADYMRSLYERITKHEIKMKEDDLRLQQKQYANSNRMLGLDGILNIVI-RKQW 855

Query: 865  STDAKSESEAIVKQTQVIFRNQGGK-RGIFYTSQRIELVRPMVEAVGWPLLATFSVTMEE 924
                   S+ ++K  Q  F+ +  K    +Y +  + ++R M+EA   P+LA FSV +++
Sbjct: 856  GDSYAETSDDLMKHMQEQFKEKARKSESTYYAATDVVILRFMIEACWAPMLAAFSVPLDQ 915

Query: 925  GDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTL 984
             D+   + +C+EGF   IH T ++ M T R AF+TSL +FT LH+P +++ +N+EA++ +
Sbjct: 916  SDDLIVINICLEGFHHAIHATSLMSMKTHRDAFVTSLAKFTSLHSPADIKQRNIEAIKAI 975

Query: 985  LILCDLETESLQDTWNAVLECVSRLE--------------FITSTPS------------- 1044
            L L D E   LQD W  +L CVSR E              F  S  +             
Sbjct: 976  LRLADEEGNYLQDAWEHILTCVSRFEQLHLLGEGAPPDATFFASKQNESEKSKQPKQYIL 1035

Query: 1045 -------------IASTVMYGS--------------NQISRDAVVQSLKELSG-KPADQV 1104
                          A+ V+ GS               Q    ++V +L  L      +QV
Sbjct: 1036 PVLKRKGPGKSQYAATGVLRGSYDSMSLGGKGSKNVRQEQMSSIVSNLNLLEQVGEMNQV 1095

Query: 1105 FVNSVKLPSDSVVEFFTALCGVSAEELKQ-TPARVFSLQKLVEISYYNMARIRMVWARIW 1164
            F  S KL S+++++F  ALC VS +EL+  +  RVFSL K+VEI++YNM RIR+VW+ IW
Sbjct: 1096 FSQSQKLNSEAIIDFVKALCKVSMDELRSPSNPRVFSLTKIVEIAHYNMNRIRLVWSSIW 1155

Query: 1165 SVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSH 1224
             VLS  F++ G   +  IA++A+DSLRQL MK+LER ELAN+ FQN+ + PFV++MR S+
Sbjct: 1156 QVLSGFFVTIGCSENLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMTPFVIVMRRSN 1215

Query: 1225 SESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEH 1284
               IR LI+ C+ QM+ S+V N+KSGW+S+FM+FT +A D+ ++IV  +FE +E++I E+
Sbjct: 1216 DVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVFLSFEIIEKIIREY 1275

Query: 1285 FDQVV---GDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAE------------ 1344
            F  +       F DCVNCL+ F NN+ S  ISL +IA LR C  +LAE            
Sbjct: 1276 FPYITETETTTFTDCVNCLVAFTNNRFSKDISLSSIAFLRYCATKLAEGDLNSPSTNKYK 1335

Query: 1345 ---GLIPGGALKPIHDSADPAFDVTE----HYWFPMLAGLSDLTSDSRPEVRSCALEVLF 1404
               G IP  +L           ++      ++WFP+L+GLS+L+ D RPE+R  AL+++F
Sbjct: 1336 GTSGKIPQSSLHSGKSGKQENGEIVNNNHLYFWFPLLSGLSELSFDPRPEIRKSALQIMF 1395

Query: 1405 DLLNERGAKFSTSFWESIFHRVLFPIFDHLRQ----PGKENLITSG-----------DEW 1464
            D L   G  FS   WE +F  VLFPIFD++R      G++     G           D W
Sbjct: 1396 DTLRNHGHLFSLPLWEKVFESVLFPIFDYVRHSIDPSGEDESADQGSSGGEVDELDHDAW 1455

Query: 1465 LRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVG 1524
            L ET   +LQL+ +LF  FY  V  +L  +L LL+   K+P QS+  + + A V L+   
Sbjct: 1456 LYETCTLALQLVVDLFVKFYTTVNPLLEKVLMLLVSFIKRPHQSLAGIGIAAFVRLMSDA 1515

Query: 1525 GHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKN 1584
               FSE+ W  ++ ++++A+ TT P               + +        + SQ+ A N
Sbjct: 1516 DGLFSEEKWLEVVSALKEAAKTTCP---------------DFSYFLSEEYVARSQRSALN 1575

Query: 1585 HQFDVGDHGKASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQ 1644
             Q    +   A+P A+   E                                       Q
Sbjct: 1576 IQNSNAE--SAAPTATDGNE-------------------------------------ESQ 1635

Query: 1645 RSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLM 1704
            R+ T              + +  S +K RA+                             
Sbjct: 1636 RTAT-------------HLYAAISDAKCRAA----------------------------- 1695

Query: 1705 AIVRGKCITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRM 1764
                     QLLL+  +  I   Y  +L+A   + ++D L  +   A   NS   LR R+
Sbjct: 1696 --------VQLLLIQAVMEIYNMYRPQLSAKNTLVLVDALHGVALHAHGINSNTILRSRL 1755

Query: 1765 HHIPD----ERPPLNLLRQELAGTSVYLDILLK-ATSRFNTLEAEEQKFVDNLEVETASP 1781
              +      + PP  LLR E     + L  L      +    E EE++ +++L V     
Sbjct: 1756 QELGPMTQMQDPP--LLRLENESYQICLTFLQNLVADKTKKEEEEEEEEIESLLVNICQE 1789

BLAST of MC05g0262 vs. ExPASy Swiss-Prot
Match: F4JSZ5 (Brefeldin A-inhibited guanine nucleotide-exchange protein 1 OS=Arabidopsis thaliana OX=3702 GN=BIG1 PE=2 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.1e-239
Identity = 522/1395 (37.42%), Postives = 775/1395 (55.56%), Query Frame = 0

Query: 106  LEGADAEIVLNPLRLAFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDIL 165
            L  +DA+ VL PL L+ DT   KVIE +LDC  KL +   L G+  +       +   ++
Sbjct: 63   LTTSDADAVLQPLLLSLDTGYAKVIEPALDCSFKLFSLSLLRGE--VCSSSPDSLLYKLI 122

Query: 166  NMICGCVDNSSSDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQA 225
            + IC  V     +S  L VL+VLL AV S +  + G+ LL ++R CYN+ L   +  NQ 
Sbjct: 123  HAICK-VCGIGEESIELAVLRVLLAAVRSPRILIRGDCLLHLVRTCYNVYLGGFNGTNQI 182

Query: 226  TSKAMLTQMISIIFRRMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGD 285
             +K++L Q++ I+F R E + +  S             +  V +  A+TD+N  E   G+
Sbjct: 183  CAKSVLAQIMLIVFTRSEANSMDASLK-----------TVNVNDLLAITDKNVNE---GN 242

Query: 286  ALNSVKDTSLASVEELQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDA 345
            +++ +    +  V      A   D   ++   + A  TED    S+         ++ D 
Sbjct: 243  SVH-ICQGFINDVITAGEAAPPPDFALVQPPEEGASSTEDEGTGSK---------IREDG 302

Query: 346  LLVFRTICKMGMKEDA-----DEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAY 405
             L+F+ +CK+ MK  +     D++  + + LSLELL+ +++     +  D  F++++K  
Sbjct: 303  FLLFKNLCKLSMKFSSQENTDDQILVRGKTLSLELLKVIIDNGGPIWLSDERFLNAIKQL 362

Query: 406  LSYALLRASVSQSPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFP-VN 465
            L  +LL+ S      IFQ    IF+ LL ++R  +K E+GIFFP++VLR L+    P   
Sbjct: 363  LCLSLLKNSALSVMSIFQLQCAIFTTLLRKYRSGMKSEVGIFFPMLVLRVLENVLQPSFV 422

Query: 466  QKISVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAV 525
            QK++VL +LE IC +P +++DIFVN+DCD+E+PN+FER+V  L K + G       +++ 
Sbjct: 423  QKMTVLSLLENICHDPNLIIDIFVNFDCDVESPNIFERIVNGLLKTALGPPPGSSTILSP 482

Query: 526  SQTTSIKGSSLQCLVNVLKSLVDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLT 585
             Q  + +  S++CLV+++K++  W   +L S   S   +SLE E+    +      +  T
Sbjct: 483  VQDITFRHESVKCLVSIIKAMGTWMDQQL-SVGDSLLPKSLENEAPANNHSNSNEEDGTT 542

Query: 586  ----------------SNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSV 645
                            +  E+ +A+K   +  ++ FNRKP KG+E+LIS+K V N+P  V
Sbjct: 543  IDHDFHPDLNPESSDAATLEQRRAYKIERQKGVTLFNRKPSKGIEFLISSKKVGNSPDEV 602

Query: 646  AQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMQFSGMKFDAAIRDFLKGFRLPGE 705
              FLRNT  L+ TMIGDYLG+ E+FP+ VMHAYVDS  F  M F  AIR FL+GFRLPGE
Sbjct: 603  VSFLRNTTGLNATMIGDYLGEREDFPMKVMHAYVDSFDFKEMNFGEAIRFFLRGFRLPGE 662

Query: 706  AQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSRPDFIRM 765
            AQKIDRIMEKFAER+C  NP  F +ADTAYVLAY+VIMLNTDAHN MV  KM++ DFIR 
Sbjct: 663  AQKIDRIMEKFAERFCKCNPNSFSSADTAYVLAYSVIMLNTDAHNIMVKEKMTKADFIRN 722

Query: 766  NVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRGKNRREG-EERGGLVSILNLALPR 825
            N   D  +  P E L  +YD +V  EIKM  D+S     +  G  +  GL  ILNL    
Sbjct: 723  NRGIDDGKDLPEEYLGALYDQVVINEIKMSSDSSAPESRQSNGLNKLLGLDGILNLVY-W 782

Query: 826  RQSSTDAKSESEAIVKQTQVIFRNQGGK-RGIFYTSQRIELVRPMVEAVGWPLLATFSVT 885
             Q+   A   +  ++K  Q  FR++ GK    ++    + ++R MVE    P+LA FSVT
Sbjct: 783  TQTEEKAVGANGLLIKDIQEKFRSKSGKSESAYHVVTDVAILRFMVEVSWGPMLAAFSVT 842

Query: 886  MEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEAL 945
            +++ D++   V C+ GFR  +H+T V+GM T R AF+TS+ +FT LH   +M+ KNV+A+
Sbjct: 843  LDQSDDRLAAVECLRGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNLHCAGDMKQKNVDAV 902

Query: 946  RTLLILCDLETESLQDTWNAVLECVSRLEFI----TSTPSIAS----------------- 1005
            + ++ +   +   LQD W  +L C+SR+E +       PS AS                 
Sbjct: 903  KAIISIAIEDGNHLQDAWEHILTCLSRIEHLQLLGEGAPSDASYFASTETEEKKALGFPN 962

Query: 1006 ---------TVM--------YGSNQISRD--------------AVVQSLKELSGKPADQV 1065
                      VM        Y S+ I  +              A +  L ++     + V
Sbjct: 963  LKKKGALQNPVMMAVVRGGSYDSSTIGPNMPGLVKQDQINNFIANLNLLDQIGSFQLNNV 1022

Query: 1066 FVNSVKLPSDSVVEFFTALCGVSAEELKQ-TPARVFSLQKLVEISYYNMARIRMVWARIW 1125
            + +S +L ++++V F  ALC VS  EL+  T  RVFSL KLVEI++YNM RIR+VW+RIW
Sbjct: 1023 YAHSQRLKTEAIVAFVKALCKVSMSELQSPTDPRVFSLTKLVEIAHYNMNRIRLVWSRIW 1082

Query: 1126 SVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSH 1185
            S+LS+ F+S G   +  +A++ +DSLRQL MK+LER ELAN+ FQN+ L+PFV++M+ S 
Sbjct: 1083 SILSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANYNFQNEFLRPFVIVMQKSS 1142

Query: 1186 SESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEH 1245
            S  IR LIV CI QM+ S+V N+KSGW+SVF +FT +A DE ++IV  AFE +E+++ E+
Sbjct: 1143 SAEIRELIVRCISQMVLSRVSNVKSGWKSVFKVFTTAAADERKNIVLLAFETMEKIVREY 1202

Query: 1246 FDQVV---GDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLI-------PG 1305
            F  +       F DCV CLI F N+  +  +SL AIA LR C  +LA+G +         
Sbjct: 1203 FSYITETEATTFTDCVRCLITFTNSTFTSDVSLNAIAFLRFCALKLADGGLVWNEKGRSS 1262

Query: 1306 GALKPIHDSADPA----FDVTEH--YWFPMLAGLSDLTSDSRPEVRSCALEVLFDLLNER 1365
                P+ D   P+     D  E+  YW P+L GLS LTSDSR  +R  +LEVLF++L + 
Sbjct: 1263 SPSTPVTDDHSPSTQNFMDADENISYWVPLLTGLSKLTSDSRSAIRKSSLEVLFNILKDH 1322

Query: 1366 GAKFSTSFWESIFHRVLFPIF-------------DHLRQPGKENLITSGDEWLRETSIHS 1395
            G  FS +FW  +F  V++PIF             +H   P   +   S   W  ETS  +
Sbjct: 1323 GHIFSRTFWIGVFSSVIYPIFNSVWGENDLLSKDEHSSFPSTFSSHPSEVSWDAETSAMA 1382

BLAST of MC05g0262 vs. ExPASy Swiss-Prot
Match: F4JN05 (Brefeldin A-inhibited guanine nucleotide-exchange protein 4 OS=Arabidopsis thaliana OX=3702 GN=BIG4 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.4e-229
Identity = 512/1410 (36.31%), Postives = 775/1410 (54.96%), Query Frame = 0

Query: 93   EPISVVLANAGHVLEGADAEIVLNPLRLAFDTKNLKVIELSLDCLHKLIAYDHLEGDPGL 152
            +P SVV       L  +DA+ VL P  L+ +T   KV+E SLDC  KL +   L G+  +
Sbjct: 56   DPSSVVSG-----LAASDADSVLQPFLLSLETAYSKVVEPSLDCAFKLFSLSILRGE--I 115

Query: 153  EGGKNVPIFTDILNMICGCVDNSSSDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCY 212
            +  K   I   ++N +   V   + +   L VL+VLL AV S    + G+ LL V++ CY
Sbjct: 116  QSSKQDSILFKLVNAV-SKVGAIAEEPIQLAVLRVLLAAVRSPCILIRGDCLLHVVKTCY 175

Query: 213  NIALHSKSPINQATSKAMLTQMISIIFRRMETDQVSLSTSSGTKESISAETSSKVVEETA 272
            NI L   S   Q  +K++L QM+ +IF R E D + +S  +     +   T   V E ++
Sbjct: 176  NIYLGGLSGTTQICAKSVLAQMMLVIFTRSEEDSLDVSVKTIYVNELLTFTDKSVNEGSS 235

Query: 273  VTDENDKETTLGDALNSVKDTSLASVEELQNLAGGADIKGLEAVLDKAVHTEDGKKMSRG 332
            V      +  + + + + + + L   + +Q L    +    E V+     +  G   +  
Sbjct: 236  V---YFCQGFVNEVMAAGQGSPLPPPDVIQILLQNPE---TETVMTPDSPSFRGYVANGE 295

Query: 333  IDLES--VNIVKRDALLVFRTICKMGMK-----EDADEVTTKTRILSLELLQGLLEGVSQ 392
             D E+  ++ V++DA L+F+ +CK+ M+      + D++  + + LSLELL+ +++    
Sbjct: 296  GDSETGDMSKVRQDAFLLFKNLCKLSMRFSSKENNDDQIMVRGKTLSLELLKVIIDNGGS 355

Query: 393  TFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYATGIFSVLLLRFRESLKGEIGIFFPL 452
             +  +  FI++VK YL  +LL+ S      IFQ    IF  LL + R  LK EIGIFFP+
Sbjct: 356  VWRTNESFINAVKQYLCLSLLKNSAVSIMSIFQLQCAIFMSLLSKLRSVLKAEIGIFFPM 415

Query: 453  IVLRSLDGTDFP-VNQKISVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSK 512
            IVLR L+    P   QK++VL +L+K+ ++PQ++VDIFVNYDCD+E+ N+ ER+V  L K
Sbjct: 416  IVLRVLENVLQPSYLQKMTVLNLLDKMSQDPQLMVDIFVNYDCDVESSNILERIVNGLLK 475

Query: 513  ISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSLVDWEKSRLQSD-----KQSENVRS 572
             + G        ++ +Q ++ +  S++CLVN+ K++ +W   +L+ +     K S+   S
Sbjct: 476  TALGPPTGSSTTLSPAQDSTFRNDSVKCLVNLAKAMGNWMDQQLKVNETVWPKGSQVYAS 535

Query: 573  LEEESSGYENLE-----IKSREDLT-------SNFEKAKAHKSTVEAAISEFNRKPVKGV 632
            ++  +S    LE       S+ D +       S  E+ +A+K  ++  IS FNRKP KGV
Sbjct: 536  MDSNASQISELEGTISDCDSQPDTSNPEAYDASMLEQRRAYKIELQKGISLFNRKPSKGV 595

Query: 633  EYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMQFSGMKF 692
            E+LIS K + ++P  VA FL  T  L+ T+IGDYLG+ +E P+ VMHAYVDS  F    F
Sbjct: 596  EFLISTKKIGSSPEEVASFLMKTAGLNGTVIGDYLGERDELPLKVMHAYVDSFNFEKKDF 655

Query: 693  DAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVIMLNTDAH 752
              AIR FL+GFRLPGEAQKIDRIMEKFAE Y   NPG F +ADTAYVLAY+VIMLNTDAH
Sbjct: 656  VEAIRFFLRGFRLPGEAQKIDRIMEKFAEHYWKCNPGSFTSADTAYVLAYSVIMLNTDAH 715

Query: 753  NPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDT-SDRGKNRREG 812
            N MV  KM++ DF+R N   D  +  P E L  +YD +VKEEI+M  DT + + K     
Sbjct: 716  NNMVKDKMTKADFVRNNRGIDDGKDLPEEYLGSLYDRVVKEEIRMNSDTLAPQNKQVNGL 775

Query: 813  EERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGK-RGIFYTSQRIELVRP 872
             +  GL  ILNL +   Q        +  +++  Q  F+ +  K   +++T   I ++R 
Sbjct: 776  NKLLGLDGILNL-VSWMQPDEKPHGANGRLIRDIQEQFQAKPEKSESVYHTVTDISILRF 835

Query: 873  MVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFT 932
            ++E    P+LA FSVT+++ D++    LC++GFR  +H+T V+GM T R AF+TS+ +FT
Sbjct: 836  ILEVSWGPMLAAFSVTIDQSDDRLATSLCLQGFRYAVHVTAVMGMQTQRDAFVTSMAKFT 895

Query: 933  FLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFIT------------ 992
             LH   +M+ KNV+A++ ++ +   +   L  +W  +L C+SR+E +             
Sbjct: 896  NLHCAADMKQKNVDAVKAIITIAIEDGNHLHGSWEHILTCLSRIEHLQLLGEVSPSEKRY 955

Query: 993  -------------------------STPSIASTVMYGS----------------NQI-SR 1052
                                       PS+ + V  GS                 QI S 
Sbjct: 956  VPTKKAEVDDKKALGFPNLKKRGSFQNPSVMAVVRGGSYDSTSLVKSVPKLVTPEQIKSF 1015

Query: 1053 DAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQ-TPARVFSLQKLV 1112
             A +  L ++     + V+ NS +L S+++V F  ALC VS  EL+  T  RVFSL KLV
Sbjct: 1016 IANLNLLDQIGNFELNHVYANSQRLNSEAIVSFVKALCKVSMSELQSPTDPRVFSLTKLV 1075

Query: 1113 EISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANF 1172
            E ++YNM RIR+VW+RIW+VLS+ F+S G   +  +A++ +DSLRQL MK+LER ELAN+
Sbjct: 1076 ETAHYNMNRIRLVWSRIWNVLSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANY 1135

Query: 1173 TFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDES 1232
             FQ++ L+PFVV+M+ S S  IR LIV C+ QM+ S+V N+KSGW++VF +FT +A DE 
Sbjct: 1136 HFQHEFLRPFVVVMQKSSSAEIRELIVRCVSQMVLSRVSNVKSGWKNVFTVFTTAALDER 1195

Query: 1233 ESIVESAFENVEQVILEHFDQVVG---DCFMDCVNCLIRFANNKSSHRISLKAIALLRIC 1292
            ++IV  AFE +E+++ +HF  ++      + DC+ CLI F N+K    I    I  LR C
Sbjct: 1196 KNIVLLAFETIEKIVRDHFHCIIETEITVYADCIRCLITFTNSKFEGDIGFNTIEFLRFC 1255

Query: 1293 EDRLAEGLI---------PGGALKPIHDSADPAFDVTEH--YWFPMLAGLSDLTSDSRPE 1352
              +L EG +            ALK          D+ E   YW P+L GL    SD RP 
Sbjct: 1256 ALKLEEGGLVLNEKLKNNTISALKEDFSDTQSFTDLDEQVSYWIPLLTGLCKQVSDPRPA 1315

Query: 1353 VRSCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLR------------QPGKENL 1395
            +R  ++EVLF +L + G  F+  FW  IF  ++ P+F+++R             P   +L
Sbjct: 1316 IRKRSIEVLFHILMDHGHLFTRPFWTGIFSSIILPVFNNIRSKTDMLFEESVDSPSSASL 1375

BLAST of MC05g0262 vs. NCBI nr
Match: XP_022146948.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1 [Momordica charantia] >XP_022146949.1 brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1 [Momordica charantia] >XP_022146950.1 brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1 [Momordica charantia])

HSP 1 Score: 3444 bits (8929), Expect = 0.0
Identity = 1781/1781 (100.00%), Postives = 1781/1781 (100.00%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG
Sbjct: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
            DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL
Sbjct: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST
Sbjct: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR
Sbjct: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE
Sbjct: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED
Sbjct: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
            ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA
Sbjct: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD
Sbjct: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL
Sbjct: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN
Sbjct: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM
Sbjct: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI
Sbjct: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780
            MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG
Sbjct: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780

Query: 781  KNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQRI 840
            KNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQRI
Sbjct: 781  KNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQRI 840

Query: 841  ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTS 900
            ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTS
Sbjct: 841  ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTS 900

Query: 901  LVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIAS 960
            LVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIAS
Sbjct: 901  LVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIAS 960

Query: 961  TVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTP 1020
            TVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTP
Sbjct: 961  TVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTP 1020

Query: 1021 ARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMK 1080
            ARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMK
Sbjct: 1021 ARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMK 1080

Query: 1081 YLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM 1140
            YLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM
Sbjct: 1081 YLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM 1140

Query: 1141 IFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKA 1200
            IFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKA
Sbjct: 1141 IFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKA 1200

Query: 1201 IALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDLTSDSRPEVRSC 1260
            IALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDLTSDSRPEVRSC
Sbjct: 1201 IALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDLTSDSRPEVRSC 1260

Query: 1261 ALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSIHSL 1320
            ALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSIHSL
Sbjct: 1261 ALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSIHSL 1320

Query: 1321 QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW 1380
            QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW
Sbjct: 1321 QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW 1380

Query: 1381 DTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVGDHG 1440
            DTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVGDHG
Sbjct: 1381 DTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVGDHG 1440

Query: 1441 KASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTIGQRI 1500
            KASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTIGQRI
Sbjct: 1441 KASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTIGQRI 1500

Query: 1501 MGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRGKCIT 1560
            MGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRGKCIT
Sbjct: 1501 MGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRGKCIT 1560

Query: 1561 QLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPDERPP 1620
            QLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPDERPP
Sbjct: 1561 QLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPDERPP 1620

Query: 1621 LNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDSSAAK 1680
            LNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDSSAAK
Sbjct: 1621 LNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDSSAAK 1680

Query: 1681 NVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCFMNSE 1740
            NVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCFMNSE
Sbjct: 1681 NVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCFMNSE 1740

Query: 1741 IFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALLS 1781
            IFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALLS
Sbjct: 1741 IFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALLS 1781

BLAST of MC05g0262 vs. NCBI nr
Match: XP_038877624.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 5 [Benincasa hispida] >XP_038877631.1 brefeldin A-inhibited guanine nucleotide-exchange protein 5 [Benincasa hispida] >XP_038877638.1 brefeldin A-inhibited guanine nucleotide-exchange protein 5 [Benincasa hispida] >XP_038877647.1 brefeldin A-inhibited guanine nucleotide-exchange protein 5 [Benincasa hispida] >XP_038877656.1 brefeldin A-inhibited guanine nucleotide-exchange protein 5 [Benincasa hispida])

HSP 1 Score: 3157 bits (8186), Expect = 0.0
Identity = 1635/1784 (91.65%), Postives = 1698/1784 (95.18%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGFV RAFESMLKECS GKKYPALQKAIQA+LD TKE NQ+Q +TP ET+QPATSAG
Sbjct: 1    MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDGTKEVNQSQ-ATPIETNQPATSAG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
            DTSETGGE DE+RT + TA+E EN GRK AP+E IS+VLANAGHVL G DAE+VLNPLRL
Sbjct: 61   DTSETGGEADESRTAQ-TAQEVENDGRKVAPREHISIVLANAGHVLHGDDAELVLNPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AFDTKNLKV+EL+LDCLHKLIAYDHLEGDPGLEGGKNV +FTDILNMICGC+DNSS DST
Sbjct: 121  AFDTKNLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISIIFR
Sbjct: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETDQVSLSTSSG K+S+SAE S  V EET V +ENDKETTL DALNSVKDTSLASVEE
Sbjct: 241  RMETDQVSLSTSSGAKDSVSAEISCTVDEETTVNEENDKETTLRDALNSVKDTSLASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            LQNLAGGADIKGLEAVLDKAVH EDGKKMSRGIDLESVNIV+RDALLVFRT+CKMGMKED
Sbjct: 301  LQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIVQRDALLVFRTLCKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
             DEVTTKTRILSLELLQGLLEGVSQ FTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA
Sbjct: 361  TDEVTTKTRILSLELLQGLLEGVSQAFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD
Sbjct: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            IFVNYDCDLEAPNLFER VTTLSKISQ TQNADPNM AVSQT+SIKGSSLQCLVNVLKSL
Sbjct: 481  IFVNYDCDLEAPNLFERTVTTLSKISQVTQNADPNMAAVSQTSSIKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEKSRL S+KQS ++ S EEESSG ENLE+KSRED+T NFEKAKAHKSTVEAAISEFN
Sbjct: 541  VDWEKSRLHSEKQSLSIHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            RKPVKGVEYLISNKLVENTPSSVAQFL+NTPSLDKTMIGDYLG HEEFPVAVMHAYVDSM
Sbjct: 601  RKPVKGVEYLISNKLVENTPSSVAQFLQNTPSLDKTMIGDYLGLHEEFPVAVMHAYVDSM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            +FSGMKFDAAIR+FLKGFRLPGEAQKIDRIMEKFAERYCA NPGLFKNADTAYVLAYAVI
Sbjct: 661  KFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780
            MLNTDAHNPMVWPKMS+ DF RMNVTNDPE+CAPTELLE+IYDSIVKEEIKMKDD  D+ 
Sbjct: 721  MLNTDAHNPMVWPKMSKSDFTRMNVTNDPEDCAPTELLEEIYDSIVKEEIKMKDDFIDKA 780

Query: 781  KNRR-EGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQR 840
            K+RR EGEERGGLVSILNLALPRR+SSTDA+SESEAI+KQTQVIFRNQG KRG+FYTSQR
Sbjct: 781  KSRRLEGEERGGLVSILNLALPRRKSSTDAQSESEAIIKQTQVIFRNQGAKRGVFYTSQR 840

Query: 841  IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT 900
            IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT
Sbjct: 841  IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT 900

Query: 901  SLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIA 960
            SLVRFTFLHAPKEMRSKNVEALRTLL LCDLETESLQDTWNAVLECVSRLEFITSTPSIA
Sbjct: 901  SLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIA 960

Query: 961  STVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT 1020
            +TVMYGSNQISRDAVVQSLKEL+GKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT
Sbjct: 961  ATVMYGSNQISRDAVVQSLKELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT 1020

Query: 1021 PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM 1080
            PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM
Sbjct: 1021 PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM 1080

Query: 1081 KYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVF 1140
            KYLERAELANFTFQNDILKPFVVLMRNS SES+RSLIVDCIVQMIKSKVGNIKSGWRSVF
Sbjct: 1081 KYLERAELANFTFQNDILKPFVVLMRNSQSESLRSLIVDCIVQMIKSKVGNIKSGWRSVF 1140

Query: 1141 MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK 1200
            MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK
Sbjct: 1141 MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK 1200

Query: 1201 AIALLRICEDRLAEGLIPGGALKPIHDS--ADPAFDVTEHYWFPMLAGLSDLTSDSRPEV 1260
            AIALLRICEDRLAEGLIPGGALKPIHD+  ADPAFD+TEHYWFPMLAGLSDLTSD RPEV
Sbjct: 1201 AIALLRICEDRLAEGLIPGGALKPIHDNDTADPAFDMTEHYWFPMLAGLSDLTSDPRPEV 1260

Query: 1261 RSCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSI 1320
            RSCALEVLFDLLNERG KFSTSFWESIFHRVLFPIFDHLR  GKE+  +SGDEWLRETSI
Sbjct: 1261 RSCALEVLFDLLNERGDKFSTSFWESIFHRVLFPIFDHLRHAGKESSTSSGDEWLRETSI 1320

Query: 1321 HSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSE 1380
            HSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQ EQSVVSLALGALVHLIEVGGHQFSE
Sbjct: 1321 HSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQLEQSVVSLALGALVHLIEVGGHQFSE 1380

Query: 1381 DDWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVG 1440
            DDWDTLLKSIRDASYTTQPLELLNALGFENP+H E+NIVDG SLKS SQQEAKNH FDVG
Sbjct: 1381 DDWDTLLKSIRDASYTTQPLELLNALGFENPNHDELNIVDGTSLKSSSQQEAKNHHFDVG 1440

Query: 1441 DHGKASPVASPR-GESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTI 1500
            DHGK SP  SPR  E ITR+PIEESGLQ TSDE AEG+PSPSTRAT+AAE G+LQRSQTI
Sbjct: 1441 DHGKVSPRLSPRVAEIITRSPIEESGLQFTSDESAEGIPSPSTRATRAAEAGNLQRSQTI 1500

Query: 1501 GQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRG 1560
            GQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPI+LPPDT+DPEVKEDEES L+ IVRG
Sbjct: 1501 GQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKEDEESPLLGIVRG 1560

Query: 1561 KCITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPD 1620
            KCITQLLLLGVIDGIQKKYW KLNAPQKIAIMDILLSLLEF+ATYNSYNNLRQRMHHIPD
Sbjct: 1561 KCITQLLLLGVIDGIQKKYWVKLNAPQKIAIMDILLSLLEFSATYNSYNNLRQRMHHIPD 1620

Query: 1621 ERPPLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDS 1680
            ERPPLNLLRQELAGTS+YLDILLKATS FNT+EA  +K V NLEV++ S + DLTS+PDS
Sbjct: 1621 ERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAGHEKIVANLEVDSESASNDLTSTPDS 1680

Query: 1681 SAAKNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCF 1740
            SA  NVDGIAENRLVSFCEQALREASDL SSAGE THMDVHRVLELRSPVIVKVI GMCF
Sbjct: 1681 SAVNNVDGIAENRLVSFCEQALREASDLQSSAGETTHMDVHRVLELRSPVIVKVINGMCF 1740

Query: 1741 MNSEIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1780
            MNS+IFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL
Sbjct: 1741 MNSQIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1782

BLAST of MC05g0262 vs. NCBI nr
Match: XP_022935314.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 5-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 3129 bits (8113), Expect = 0.0
Identity = 1610/1781 (90.40%), Postives = 1691/1781 (94.95%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGFV RAFESMLKECS GKKYPALQKAIQA+LDSTKE NQ+QHSTP ET QP TSAG
Sbjct: 1    MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDSTKEVNQSQHSTPVETIQPETSAG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
            DTSETGGE DE+ T + TA+EAEN G K AP+E IS+VLANAGHVL+GADAEIVLNPLRL
Sbjct: 61   DTSETGGEADESPTAQ-TAQEAENEGSKVAPREHISIVLANAGHVLQGADAEIVLNPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AFDTKNLKV+EL+LDCLHKLIAYDHLEGDPGLEGGKNVP+FTDILNMICGC+DNSS DST
Sbjct: 121  AFDTKNLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVPLFTDILNMICGCIDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISIIFR
Sbjct: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETDQVSLSTSSGTKESISAE SS V EE  V+DEN  ETTLGDALNSVKDTSLASVEE
Sbjct: 241  RMETDQVSLSTSSGTKESISAEISSGVDEENTVSDENGNETTLGDALNSVKDTSLASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            LQNLAGGADIKGLEAVLDKAVH+EDGKKMSRGIDLESVNIV+RDALLVFRT+CKMGMKED
Sbjct: 301  LQNLAGGADIKGLEAVLDKAVHSEDGKKMSRGIDLESVNIVQRDALLVFRTLCKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
             DEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA
Sbjct: 361  TDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            TGIFSVLLLRFR+SLKGEIGIFFPLIVLRSLDGTDFPVNQK+SVLKMLEKICREPQILVD
Sbjct: 421  TGIFSVLLLRFRDSLKGEIGIFFPLIVLRSLDGTDFPVNQKLSVLKMLEKICREPQILVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            IFVNYDCDLEAPNLFER VTTLS+ISQGTQNADPN+ AVSQTTSIKGSSLQCLVNVLKSL
Sbjct: 481  IFVNYDCDLEAPNLFERTVTTLSRISQGTQNADPNLAAVSQTTSIKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEKSRL S+KQS ++ S EEE SG ENLEIKSRED+T NFEKAKAHKSTVEA ISEFN
Sbjct: 541  VDWEKSRLHSEKQSLSIHSSEEEPSGNENLEIKSREDVTGNFEKAKAHKSTVEAVISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            RKPVKGVEYLI+NKLV NTPSSVAQFL+NTPSLDKTMIG+YLGQHEEFP+AVMHAYVD M
Sbjct: 601  RKPVKGVEYLIANKLVANTPSSVAQFLQNTPSLDKTMIGEYLGQHEEFPIAVMHAYVDCM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            +FSGMKFDAAIR+FLKGFRLPGEAQKIDRIMEKFAERYCA NPGLFKNADTAYVLAYAVI
Sbjct: 661  KFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780
            MLNTDAHNPMVWPKMS+PDF+RMNVTNDPE+CAPTELLE+IYDSIVKEEIKMKDDTSD+ 
Sbjct: 721  MLNTDAHNPMVWPKMSKPDFVRMNVTNDPEDCAPTELLEEIYDSIVKEEIKMKDDTSDKA 780

Query: 781  KNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQRI 840
            K+RREGEERGGLVSILNLALP+R+SSTDAKSESEAI+KQTQVIFRNQG KRG+FYTS+RI
Sbjct: 781  KSRREGEERGGLVSILNLALPKRKSSTDAKSESEAIIKQTQVIFRNQGAKRGVFYTSKRI 840

Query: 841  ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTS 900
            ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHIT+VLGMDTMRYAFLTS
Sbjct: 841  ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITYVLGMDTMRYAFLTS 900

Query: 901  LVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIAS 960
            LVRFTFLHAPKEMRSKNVEALRTLL LCDLETESLQDTWNAVLEC SRLEFITSTPSIA+
Sbjct: 901  LVRFTFLHAPKEMRSKNVEALRTLLDLCDLETESLQDTWNAVLECASRLEFITSTPSIAA 960

Query: 961  TVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTP 1020
            TVMYGSNQISRDAVVQSL+EL+GKPADQVFVNSV+LPSDSVVEFFTALCGVSAEELKQTP
Sbjct: 961  TVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVRLPSDSVVEFFTALCGVSAEELKQTP 1020

Query: 1021 ARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMK 1080
            ARVFSLQKLVEISYYNMARIR+VWARIWSVLSNHFI+AGSHHDEKI+MYAIDSLRQLGMK
Sbjct: 1021 ARVFSLQKLVEISYYNMARIRLVWARIWSVLSNHFIAAGSHHDEKISMYAIDSLRQLGMK 1080

Query: 1081 YLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM 1140
            YLERAELANFTFQNDILKPFV+LMRNS SESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM
Sbjct: 1081 YLERAELANFTFQNDILKPFVILMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM 1140

Query: 1141 IFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKA 1200
            IFTASADDESE+IVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKS HRISLKA
Sbjct: 1141 IFTASADDESETIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSFHRISLKA 1200

Query: 1201 IALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDLTSDSRPEVRSC 1260
            IALLRICEDRLAEGLIPGGALKPIHDS DPAFD+TEHYWFPMLAGLSDLTSD RPEVRSC
Sbjct: 1201 IALLRICEDRLAEGLIPGGALKPIHDSTDPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSC 1260

Query: 1261 ALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSIHSL 1320
            ALEVLFDLLNERGAKFSTSFWE+IFH VLFPIF HLR  GKE+L +SGDEWLRETSIHS+
Sbjct: 1261 ALEVLFDLLNERGAKFSTSFWENIFHHVLFPIFHHLRHVGKESLNSSGDEWLRETSIHSI 1320

Query: 1321 QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW 1380
            QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW
Sbjct: 1321 QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW 1380

Query: 1381 DTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVGDHG 1440
            DTLLKSIRDASYTTQPLELLNALGFENPSH EVN+V G SLKSVS  EAKN  FDV DH 
Sbjct: 1381 DTLLKSIRDASYTTQPLELLNALGFENPSHDEVNVVGGTSLKSVSPLEAKNLHFDVSDHR 1440

Query: 1441 KASPVASPRGESI-TRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTIGQR 1500
            K SP+ SPR   I TRN I ESG+QITSDE AEGLPSPSTRA   AE GSLQRSQTIGQR
Sbjct: 1441 KVSPLPSPRVAEINTRNTINESGIQITSDESAEGLPSPSTRA---AEAGSLQRSQTIGQR 1500

Query: 1501 IMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRGKCI 1560
            IMGNMMDNIFVRSLTSKSKG ASDASVPSSPIKLPPDT+DPEVK++EE  L+ IVRGKC+
Sbjct: 1501 IMGNMMDNIFVRSLTSKSKGVASDASVPSSPIKLPPDTVDPEVKDEEEIPLLGIVRGKCV 1560

Query: 1561 TQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPDERP 1620
            TQLLLLGVIDGIQKKYW KLNAPQKIAIMDILLSLLEF+ATYNSYNNLRQRMHHIP+ERP
Sbjct: 1561 TQLLLLGVIDGIQKKYWVKLNAPQKIAIMDILLSLLEFSATYNSYNNLRQRMHHIPNERP 1620

Query: 1621 PLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDSSAA 1680
            PLNLLRQELAGTS+YLDILLKATS  NT+EA ++K VD LEV+T S N  LTS+PDSSA 
Sbjct: 1621 PLNLLRQELAGTSIYLDILLKATSGINTIEAGQEKTVDKLEVKTESANDGLTSAPDSSAV 1680

Query: 1681 KNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCFMNS 1740
             +VDG+AE+RLVSFCE ALREASDL SS GEATHMDVH+VLELRSPVIVKVIKGMCFMNS
Sbjct: 1681 NDVDGVAESRLVSFCEHALREASDLQSSVGEATHMDVHQVLELRSPVIVKVIKGMCFMNS 1740

Query: 1741 EIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1780
            +IFRRHLREFYPLLTKLVCCDQID+RGALG+LFKIQLKAL+
Sbjct: 1741 QIFRRHLREFYPLLTKLVCCDQIDIRGALGELFKIQLKALV 1777

BLAST of MC05g0262 vs. NCBI nr
Match: KAA0049067.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 3127 bits (8108), Expect = 0.0
Identity = 1620/1787 (90.65%), Postives = 1693/1787 (94.74%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGFV RAFESMLKECS GKKYPALQKAIQA+LD+TKE NQ Q +TP ET+QPA SAG
Sbjct: 1    MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNQIQQATPIETNQPAASAG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
            D SETGGE DE++T + TA+E EN G+K AP+E IS+VLANAGHVL G DAE+VLNPLRL
Sbjct: 61   DNSETGGEADESQTAQ-TAQEVENNGKKVAPREHISIVLANAGHVLHGDDAELVLNPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AFDTKNLKV+EL+LDCLHKLIAYDHLEGDPGLEGGKNV +FTDILNMICGC+DNSS DST
Sbjct: 121  AFDTKNLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISIIFR
Sbjct: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETDQVSLSTSSGTK+S SAE SS V EET V +E+DKETTLGDALNSVKDTS+ASVEE
Sbjct: 241  RMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEEDDKETTLGDALNSVKDTSIASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            LQNLAGGADIKGLEAVLDKAVH EDGKKMSRGIDLESVNI++RDALLVFRT+CKMGMKED
Sbjct: 301  LQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
             DEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA
Sbjct: 361  TDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            TGIFSVLLLRFRESLKGEIGIFFPLI+LRSLDGTDFPVNQK SVLKMLEKICREPQILVD
Sbjct: 421  TGIFSVLLLRFRESLKGEIGIFFPLIILRSLDGTDFPVNQKTSVLKMLEKICREPQILVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            IFVNYDCDLEAPNLFERMVTTLSK+SQGTQNADPN+ A+SQTTSIKGSSLQCLVNVLKSL
Sbjct: 481  IFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQTTSIKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEKSRL S+KQ     S EEESSG ENLE+KSRED+TSNFEKAKAHKSTVEAAISEFN
Sbjct: 541  VDWEKSRLHSEKQG-FAHSSEEESSGNENLEVKSREDVTSNFEKAKAHKSTVEAAISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            RKPVKGVEYLI NKLVENTPSSVA FLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM
Sbjct: 601  RKPVKGVEYLILNKLVENTPSSVAHFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            +FSGMKFDAAIR+FLKGFRLPGEAQKIDRIMEKFAERYCA NPGLFKNADTAYVLAYAVI
Sbjct: 661  KFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780
            MLNTDAHNPMVWPKMS+ DF RMNV N+PE+CAPTELLE+IYDSIVKEEIKMKDD  D+ 
Sbjct: 721  MLNTDAHNPMVWPKMSKSDFTRMNVMNNPEDCAPTELLEEIYDSIVKEEIKMKDDLIDKA 780

Query: 781  KNRR-EGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQR 840
            K+RR E EE+GGLVSILNLALPRR+SSTDA+SESEAIVKQTQVIFRNQG KRG+FYTSQR
Sbjct: 781  KSRRLESEEKGGLVSILNLALPRRKSSTDAQSESEAIVKQTQVIFRNQGAKRGVFYTSQR 840

Query: 841  IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT 900
            IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT
Sbjct: 841  IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT 900

Query: 901  SLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIA 960
            SLVRFTFLHAPKEMRSKNVEALRTLL LCDLETESLQDTWNAVLECVSRLEFITSTPSIA
Sbjct: 901  SLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIA 960

Query: 961  STVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT 1020
            +TVMYGSNQISRDAVVQSLKEL+GKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT
Sbjct: 961  ATVMYGSNQISRDAVVQSLKELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT 1020

Query: 1021 PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM 1080
            PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM
Sbjct: 1021 PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM 1080

Query: 1081 KYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVF 1140
            KYLERAELANFTFQNDILKPFVVLMRNS SES+RSLIVDCIVQMIKSKVGNIKSGWRSVF
Sbjct: 1081 KYLERAELANFTFQNDILKPFVVLMRNSQSESLRSLIVDCIVQMIKSKVGNIKSGWRSVF 1140

Query: 1141 MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK 1200
            MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK
Sbjct: 1141 MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK 1200

Query: 1201 AIALLRICEDRLAEGLIPGGALKPIHD----SADPAFDVTEHYWFPMLAGLSDLTSDSRP 1260
            AIALLRICEDRLAEGLIPGGALKPIHD    SA+PAFD+TEHYWFPMLAGLSDLTSD RP
Sbjct: 1201 AIALLRICEDRLAEGLIPGGALKPIHDNDNDSAEPAFDMTEHYWFPMLAGLSDLTSDPRP 1260

Query: 1261 EVRSCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRET 1320
            EVRSCALEVLFDLLNERG KFSTSFWESIFHRVLFPIFDHLR  GKE++ +SGDEWLRET
Sbjct: 1261 EVRSCALEVLFDLLNERGDKFSTSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRET 1320

Query: 1321 SIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQF 1380
            SIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQF
Sbjct: 1321 SIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQF 1380

Query: 1381 SEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFD 1440
            SEDDWDTLLKSIRDASYTTQPLELLNALGFENPSH E+NIVD  SLK  SQQEAKNH  D
Sbjct: 1381 SEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHID 1440

Query: 1441 VGDHGKASPVASPR-GESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQ 1500
            V DHGK SPV SPR  E ITR+PI ESGLQIT+DE AEG+PSPSTRAT+AAE G+LQRSQ
Sbjct: 1441 VSDHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAGNLQRSQ 1500

Query: 1501 TIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIV 1560
            TIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPI+LPPD +DPEVK+DEES L+ IV
Sbjct: 1501 TIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDAVDPEVKDDEESPLLGIV 1560

Query: 1561 RGKCITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHI 1620
            RGKCITQLLLLGVIDGIQKKYW KLNAPQKIAIMDILLSLLEF+ATYNSYNNLRQRM+HI
Sbjct: 1561 RGKCITQLLLLGVIDGIQKKYWVKLNAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHI 1620

Query: 1621 PDE-RPPLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSS 1680
            PDE RPPLNLLRQELAGTS+YLDILLKATS FN++EAE++K VD+LEV++ SP  DLTS+
Sbjct: 1621 PDESRPPLNLLRQELAGTSIYLDILLKATSGFNSIEAEQKKIVDSLEVDSESPKDDLTST 1680

Query: 1681 PDSSAAKNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKG 1740
             DSSA  NVD IAENRLVSFCEQALRE SDL SSA E THMDVHRVLELRSPVIVKVIKG
Sbjct: 1681 QDSSAVNNVDRIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKG 1740

Query: 1741 MCFMNSEIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1780
            MCFMNS+IFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL
Sbjct: 1741 MCFMNSQIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1785

BLAST of MC05g0262 vs. NCBI nr
Match: XP_004134353.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1 [Cucumis sativus] >XP_011650809.1 brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1 [Cucumis sativus] >XP_011650810.1 brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1 [Cucumis sativus] >XP_031737831.1 brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1 [Cucumis sativus] >KGN56635.1 hypothetical protein Csa_010391 [Cucumis sativus])

HSP 1 Score: 3124 bits (8100), Expect = 0.0
Identity = 1614/1784 (90.47%), Postives = 1694/1784 (94.96%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGFV RAFESMLKECS GKKYPALQKAIQA+LD+TKE N++Q +TP ET+QPA SAG
Sbjct: 1    MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNRSQQATPIETNQPAASAG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
            DTSETGGE DE++T + +A+E EN G+KAAP+E IS+VLANAGHVL G DAE+VL+PLRL
Sbjct: 61   DTSETGGEADESQTAQ-SAQEVENNGKKAAPREHISIVLANAGHVLHGDDAELVLSPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AFDTK+LKV+EL+LDCLHKLIAYDHLEGDPGLEGGKNV +FTDILNMICGC+DNSS DST
Sbjct: 121  AFDTKHLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISIIFR
Sbjct: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETDQVSLSTSSGTK+S SAE SS V EET V +ENDKETTLGDALNSVKDTS+ASVEE
Sbjct: 241  RMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEENDKETTLGDALNSVKDTSIASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            LQNLAGGADIKGLEAVLDKAVH EDGKKMSRGIDLESVNI++RDALLVFRT+CKMGMKED
Sbjct: 301  LQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
             DEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQ PVIFQYA
Sbjct: 361  TDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQK SVLKMLEKICREPQILVD
Sbjct: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            IFVNYDCDLEAPNLFERMVTTLSK+SQGTQNADPN+ A+SQ TSIKGSSLQCLVNVLKSL
Sbjct: 481  IFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEKSRL S+K+   V S EEESSG ENLE+KSRED+T NFEKAKAHKSTVEAAISEFN
Sbjct: 541  VDWEKSRLHSEKEG-LVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            RKPVKGVEYLISNKLVENTPSSVA FLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM
Sbjct: 601  RKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            +FSGMKFDAAIR+FLKGFRLPGEAQKIDRIMEKFAERYCA NPGLFKNADTAYVLAYAVI
Sbjct: 661  KFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780
            MLNTDAHNPMVWPKMS+ DF RMNV NDPE+CAPTELLE+IYDSIVKEEIKMKDD  D+ 
Sbjct: 721  MLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKA 780

Query: 781  KNRR-EGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQR 840
            K+RR E EE+GGLVSILNLALPRR+SST+A+SESEAI+KQTQVIFRNQG KRG+FYTSQR
Sbjct: 781  KSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQR 840

Query: 841  IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT 900
            IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT
Sbjct: 841  IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT 900

Query: 901  SLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIA 960
            SLVRFTFLHAPKEMRSKNVEALRTLL LCDLETESLQDTWNAVLECVSRLEFITSTPSIA
Sbjct: 901  SLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIA 960

Query: 961  STVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT 1020
            +TVMYGSNQISRDAVVQSL+EL+GKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT
Sbjct: 961  ATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT 1020

Query: 1021 PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM 1080
            PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM
Sbjct: 1021 PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM 1080

Query: 1081 KYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVF 1140
            KYLERAELANFTFQNDILKPFVVLMRNS SESIRSLIVDCIVQMIKSKVGNIKSGWRSVF
Sbjct: 1081 KYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVF 1140

Query: 1141 MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK 1200
            MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK
Sbjct: 1141 MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK 1200

Query: 1201 AIALLRICEDRLAEGLIPGGALKPIHD--SADPAFDVTEHYWFPMLAGLSDLTSDSRPEV 1260
            AIALLRICEDRLAEGLIPGGALKPIHD  SA+PAFD+TEHYWFPMLAGLSDLTSD RPEV
Sbjct: 1201 AIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEV 1260

Query: 1261 RSCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSI 1320
            RSCALEVLFDLLNERG+KFS SFWESIFHRVLFPIFDHLR  GKE++ +SGDEWLRETSI
Sbjct: 1261 RSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSI 1320

Query: 1321 HSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSE 1380
            HSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSE
Sbjct: 1321 HSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSE 1380

Query: 1381 DDWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVG 1440
            DDWDTLLKSIRDASYTTQPLELLNALGFENPSH E+NIVD  SLK  SQQEAKNH  DV 
Sbjct: 1381 DDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVN 1440

Query: 1441 DHGKASPVASPR-GESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTI 1500
            +HGK SPV SPR  E ITR+PI ESGLQIT+DE AEG+PSPSTRAT+AAE  +LQRSQTI
Sbjct: 1441 EHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTI 1500

Query: 1501 GQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRG 1560
            GQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPI+LPPDT+DPEVK+DEES L+ IVRG
Sbjct: 1501 GQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRG 1560

Query: 1561 KCITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPD 1620
            KCITQLLLLGVIDGIQKKYW KL+APQKIAIMDILLSLLEF+ATYNSYNNLRQRM+HIPD
Sbjct: 1561 KCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPD 1620

Query: 1621 ERPPLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDS 1680
            ERPPLNLLRQELAGTS+YLDILLKATS FNT+EAE++K  D+LEV++ SP  DLTS  DS
Sbjct: 1621 ERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDS 1680

Query: 1681 SAAKNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCF 1740
            SA  NVDGIAENRLVSFCEQALRE SDL SSA E THMDVHRVLELRSPVIVKVIKGMCF
Sbjct: 1681 SAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCF 1740

Query: 1741 MNSEIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1780
            MNS+IFRRHLREFYPLLTKLVCCDQID+RGALGDLFKIQLKALL
Sbjct: 1741 MNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALL 1782

BLAST of MC05g0262 vs. ExPASy TrEMBL
Match: A0A6J1CYS8 (brefeldin A-inhibited guanine nucleotide-exchange protein 5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016023 PE=4 SV=1)

HSP 1 Score: 3444 bits (8929), Expect = 0.0
Identity = 1781/1781 (100.00%), Postives = 1781/1781 (100.00%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG
Sbjct: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
            DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL
Sbjct: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST
Sbjct: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR
Sbjct: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE
Sbjct: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED
Sbjct: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
            ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA
Sbjct: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD
Sbjct: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL
Sbjct: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN
Sbjct: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM
Sbjct: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI
Sbjct: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780
            MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG
Sbjct: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780

Query: 781  KNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQRI 840
            KNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQRI
Sbjct: 781  KNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQRI 840

Query: 841  ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTS 900
            ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTS
Sbjct: 841  ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTS 900

Query: 901  LVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIAS 960
            LVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIAS
Sbjct: 901  LVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIAS 960

Query: 961  TVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTP 1020
            TVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTP
Sbjct: 961  TVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTP 1020

Query: 1021 ARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMK 1080
            ARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMK
Sbjct: 1021 ARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMK 1080

Query: 1081 YLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM 1140
            YLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM
Sbjct: 1081 YLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM 1140

Query: 1141 IFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKA 1200
            IFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKA
Sbjct: 1141 IFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKA 1200

Query: 1201 IALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDLTSDSRPEVRSC 1260
            IALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDLTSDSRPEVRSC
Sbjct: 1201 IALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDLTSDSRPEVRSC 1260

Query: 1261 ALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSIHSL 1320
            ALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSIHSL
Sbjct: 1261 ALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSIHSL 1320

Query: 1321 QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW 1380
            QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW
Sbjct: 1321 QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW 1380

Query: 1381 DTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVGDHG 1440
            DTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVGDHG
Sbjct: 1381 DTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVGDHG 1440

Query: 1441 KASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTIGQRI 1500
            KASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTIGQRI
Sbjct: 1441 KASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTIGQRI 1500

Query: 1501 MGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRGKCIT 1560
            MGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRGKCIT
Sbjct: 1501 MGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRGKCIT 1560

Query: 1561 QLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPDERPP 1620
            QLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPDERPP
Sbjct: 1561 QLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPDERPP 1620

Query: 1621 LNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDSSAAK 1680
            LNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDSSAAK
Sbjct: 1621 LNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDSSAAK 1680

Query: 1681 NVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCFMNSE 1740
            NVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCFMNSE
Sbjct: 1681 NVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCFMNSE 1740

Query: 1741 IFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALLS 1781
            IFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALLS
Sbjct: 1741 IFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALLS 1781

BLAST of MC05g0262 vs. ExPASy TrEMBL
Match: A0A6J1F528 (brefeldin A-inhibited guanine nucleotide-exchange protein 5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442227 PE=4 SV=1)

HSP 1 Score: 3129 bits (8113), Expect = 0.0
Identity = 1610/1781 (90.40%), Postives = 1691/1781 (94.95%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGFV RAFESMLKECS GKKYPALQKAIQA+LDSTKE NQ+QHSTP ET QP TSAG
Sbjct: 1    MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDSTKEVNQSQHSTPVETIQPETSAG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
            DTSETGGE DE+ T + TA+EAEN G K AP+E IS+VLANAGHVL+GADAEIVLNPLRL
Sbjct: 61   DTSETGGEADESPTAQ-TAQEAENEGSKVAPREHISIVLANAGHVLQGADAEIVLNPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AFDTKNLKV+EL+LDCLHKLIAYDHLEGDPGLEGGKNVP+FTDILNMICGC+DNSS DST
Sbjct: 121  AFDTKNLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVPLFTDILNMICGCIDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISIIFR
Sbjct: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETDQVSLSTSSGTKESISAE SS V EE  V+DEN  ETTLGDALNSVKDTSLASVEE
Sbjct: 241  RMETDQVSLSTSSGTKESISAEISSGVDEENTVSDENGNETTLGDALNSVKDTSLASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            LQNLAGGADIKGLEAVLDKAVH+EDGKKMSRGIDLESVNIV+RDALLVFRT+CKMGMKED
Sbjct: 301  LQNLAGGADIKGLEAVLDKAVHSEDGKKMSRGIDLESVNIVQRDALLVFRTLCKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
             DEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA
Sbjct: 361  TDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            TGIFSVLLLRFR+SLKGEIGIFFPLIVLRSLDGTDFPVNQK+SVLKMLEKICREPQILVD
Sbjct: 421  TGIFSVLLLRFRDSLKGEIGIFFPLIVLRSLDGTDFPVNQKLSVLKMLEKICREPQILVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            IFVNYDCDLEAPNLFER VTTLS+ISQGTQNADPN+ AVSQTTSIKGSSLQCLVNVLKSL
Sbjct: 481  IFVNYDCDLEAPNLFERTVTTLSRISQGTQNADPNLAAVSQTTSIKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEKSRL S+KQS ++ S EEE SG ENLEIKSRED+T NFEKAKAHKSTVEA ISEFN
Sbjct: 541  VDWEKSRLHSEKQSLSIHSSEEEPSGNENLEIKSREDVTGNFEKAKAHKSTVEAVISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            RKPVKGVEYLI+NKLV NTPSSVAQFL+NTPSLDKTMIG+YLGQHEEFP+AVMHAYVD M
Sbjct: 601  RKPVKGVEYLIANKLVANTPSSVAQFLQNTPSLDKTMIGEYLGQHEEFPIAVMHAYVDCM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            +FSGMKFDAAIR+FLKGFRLPGEAQKIDRIMEKFAERYCA NPGLFKNADTAYVLAYAVI
Sbjct: 661  KFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780
            MLNTDAHNPMVWPKMS+PDF+RMNVTNDPE+CAPTELLE+IYDSIVKEEIKMKDDTSD+ 
Sbjct: 721  MLNTDAHNPMVWPKMSKPDFVRMNVTNDPEDCAPTELLEEIYDSIVKEEIKMKDDTSDKA 780

Query: 781  KNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQRI 840
            K+RREGEERGGLVSILNLALP+R+SSTDAKSESEAI+KQTQVIFRNQG KRG+FYTS+RI
Sbjct: 781  KSRREGEERGGLVSILNLALPKRKSSTDAKSESEAIIKQTQVIFRNQGAKRGVFYTSKRI 840

Query: 841  ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTS 900
            ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHIT+VLGMDTMRYAFLTS
Sbjct: 841  ELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITYVLGMDTMRYAFLTS 900

Query: 901  LVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIAS 960
            LVRFTFLHAPKEMRSKNVEALRTLL LCDLETESLQDTWNAVLEC SRLEFITSTPSIA+
Sbjct: 901  LVRFTFLHAPKEMRSKNVEALRTLLDLCDLETESLQDTWNAVLECASRLEFITSTPSIAA 960

Query: 961  TVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQTP 1020
            TVMYGSNQISRDAVVQSL+EL+GKPADQVFVNSV+LPSDSVVEFFTALCGVSAEELKQTP
Sbjct: 961  TVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVRLPSDSVVEFFTALCGVSAEELKQTP 1020

Query: 1021 ARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGMK 1080
            ARVFSLQKLVEISYYNMARIR+VWARIWSVLSNHFI+AGSHHDEKI+MYAIDSLRQLGMK
Sbjct: 1021 ARVFSLQKLVEISYYNMARIRLVWARIWSVLSNHFIAAGSHHDEKISMYAIDSLRQLGMK 1080

Query: 1081 YLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM 1140
            YLERAELANFTFQNDILKPFV+LMRNS SESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM
Sbjct: 1081 YLERAELANFTFQNDILKPFVILMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVFM 1140

Query: 1141 IFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLKA 1200
            IFTASADDESE+IVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKS HRISLKA
Sbjct: 1141 IFTASADDESETIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSFHRISLKA 1200

Query: 1201 IALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDLTSDSRPEVRSC 1260
            IALLRICEDRLAEGLIPGGALKPIHDS DPAFD+TEHYWFPMLAGLSDLTSD RPEVRSC
Sbjct: 1201 IALLRICEDRLAEGLIPGGALKPIHDSTDPAFDMTEHYWFPMLAGLSDLTSDPRPEVRSC 1260

Query: 1261 ALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSIHSL 1320
            ALEVLFDLLNERGAKFSTSFWE+IFH VLFPIF HLR  GKE+L +SGDEWLRETSIHS+
Sbjct: 1261 ALEVLFDLLNERGAKFSTSFWENIFHHVLFPIFHHLRHVGKESLNSSGDEWLRETSIHSI 1320

Query: 1321 QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW 1380
            QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW
Sbjct: 1321 QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSEDDW 1380

Query: 1381 DTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVGDHG 1440
            DTLLKSIRDASYTTQPLELLNALGFENPSH EVN+V G SLKSVS  EAKN  FDV DH 
Sbjct: 1381 DTLLKSIRDASYTTQPLELLNALGFENPSHDEVNVVGGTSLKSVSPLEAKNLHFDVSDHR 1440

Query: 1441 KASPVASPRGESI-TRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTIGQR 1500
            K SP+ SPR   I TRN I ESG+QITSDE AEGLPSPSTRA   AE GSLQRSQTIGQR
Sbjct: 1441 KVSPLPSPRVAEINTRNTINESGIQITSDESAEGLPSPSTRA---AEAGSLQRSQTIGQR 1500

Query: 1501 IMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRGKCI 1560
            IMGNMMDNIFVRSLTSKSKG ASDASVPSSPIKLPPDT+DPEVK++EE  L+ IVRGKC+
Sbjct: 1501 IMGNMMDNIFVRSLTSKSKGVASDASVPSSPIKLPPDTVDPEVKDEEEIPLLGIVRGKCV 1560

Query: 1561 TQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPDERP 1620
            TQLLLLGVIDGIQKKYW KLNAPQKIAIMDILLSLLEF+ATYNSYNNLRQRMHHIP+ERP
Sbjct: 1561 TQLLLLGVIDGIQKKYWVKLNAPQKIAIMDILLSLLEFSATYNSYNNLRQRMHHIPNERP 1620

Query: 1621 PLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDSSAA 1680
            PLNLLRQELAGTS+YLDILLKATS  NT+EA ++K VD LEV+T S N  LTS+PDSSA 
Sbjct: 1621 PLNLLRQELAGTSIYLDILLKATSGINTIEAGQEKTVDKLEVKTESANDGLTSAPDSSAV 1680

Query: 1681 KNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCFMNS 1740
             +VDG+AE+RLVSFCE ALREASDL SS GEATHMDVH+VLELRSPVIVKVIKGMCFMNS
Sbjct: 1681 NDVDGVAESRLVSFCEHALREASDLQSSVGEATHMDVHQVLELRSPVIVKVIKGMCFMNS 1740

Query: 1741 EIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1780
            +IFRRHLREFYPLLTKLVCCDQID+RGALG+LFKIQLKAL+
Sbjct: 1741 QIFRRHLREFYPLLTKLVCCDQIDIRGALGELFKIQLKALV 1777

BLAST of MC05g0262 vs. ExPASy TrEMBL
Match: A0A5A7U421 (Brefeldin A-inhibited guanine nucleotide-exchange protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002960 PE=4 SV=1)

HSP 1 Score: 3127 bits (8108), Expect = 0.0
Identity = 1620/1787 (90.65%), Postives = 1693/1787 (94.74%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGFV RAFESMLKECS GKKYPALQKAIQA+LD+TKE NQ Q +TP ET+QPA SAG
Sbjct: 1    MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNQIQQATPIETNQPAASAG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
            D SETGGE DE++T + TA+E EN G+K AP+E IS+VLANAGHVL G DAE+VLNPLRL
Sbjct: 61   DNSETGGEADESQTAQ-TAQEVENNGKKVAPREHISIVLANAGHVLHGDDAELVLNPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AFDTKNLKV+EL+LDCLHKLIAYDHLEGDPGLEGGKNV +FTDILNMICGC+DNSS DST
Sbjct: 121  AFDTKNLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISIIFR
Sbjct: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETDQVSLSTSSGTK+S SAE SS V EET V +E+DKETTLGDALNSVKDTS+ASVEE
Sbjct: 241  RMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEEDDKETTLGDALNSVKDTSIASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            LQNLAGGADIKGLEAVLDKAVH EDGKKMSRGIDLESVNI++RDALLVFRT+CKMGMKED
Sbjct: 301  LQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
             DEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA
Sbjct: 361  TDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            TGIFSVLLLRFRESLKGEIGIFFPLI+LRSLDGTDFPVNQK SVLKMLEKICREPQILVD
Sbjct: 421  TGIFSVLLLRFRESLKGEIGIFFPLIILRSLDGTDFPVNQKTSVLKMLEKICREPQILVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            IFVNYDCDLEAPNLFERMVTTLSK+SQGTQNADPN+ A+SQTTSIKGSSLQCLVNVLKSL
Sbjct: 481  IFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQTTSIKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEKSRL S+KQ     S EEESSG ENLE+KSRED+TSNFEKAKAHKSTVEAAISEFN
Sbjct: 541  VDWEKSRLHSEKQG-FAHSSEEESSGNENLEVKSREDVTSNFEKAKAHKSTVEAAISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            RKPVKGVEYLI NKLVENTPSSVA FLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM
Sbjct: 601  RKPVKGVEYLILNKLVENTPSSVAHFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            +FSGMKFDAAIR+FLKGFRLPGEAQKIDRIMEKFAERYCA NPGLFKNADTAYVLAYAVI
Sbjct: 661  KFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780
            MLNTDAHNPMVWPKMS+ DF RMNV N+PE+CAPTELLE+IYDSIVKEEIKMKDD  D+ 
Sbjct: 721  MLNTDAHNPMVWPKMSKSDFTRMNVMNNPEDCAPTELLEEIYDSIVKEEIKMKDDLIDKA 780

Query: 781  KNRR-EGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQR 840
            K+RR E EE+GGLVSILNLALPRR+SSTDA+SESEAIVKQTQVIFRNQG KRG+FYTSQR
Sbjct: 781  KSRRLESEEKGGLVSILNLALPRRKSSTDAQSESEAIVKQTQVIFRNQGAKRGVFYTSQR 840

Query: 841  IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT 900
            IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT
Sbjct: 841  IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT 900

Query: 901  SLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIA 960
            SLVRFTFLHAPKEMRSKNVEALRTLL LCDLETESLQDTWNAVLECVSRLEFITSTPSIA
Sbjct: 901  SLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIA 960

Query: 961  STVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT 1020
            +TVMYGSNQISRDAVVQSLKEL+GKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT
Sbjct: 961  ATVMYGSNQISRDAVVQSLKELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT 1020

Query: 1021 PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM 1080
            PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM
Sbjct: 1021 PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM 1080

Query: 1081 KYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVF 1140
            KYLERAELANFTFQNDILKPFVVLMRNS SES+RSLIVDCIVQMIKSKVGNIKSGWRSVF
Sbjct: 1081 KYLERAELANFTFQNDILKPFVVLMRNSQSESLRSLIVDCIVQMIKSKVGNIKSGWRSVF 1140

Query: 1141 MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK 1200
            MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK
Sbjct: 1141 MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK 1200

Query: 1201 AIALLRICEDRLAEGLIPGGALKPIHD----SADPAFDVTEHYWFPMLAGLSDLTSDSRP 1260
            AIALLRICEDRLAEGLIPGGALKPIHD    SA+PAFD+TEHYWFPMLAGLSDLTSD RP
Sbjct: 1201 AIALLRICEDRLAEGLIPGGALKPIHDNDNDSAEPAFDMTEHYWFPMLAGLSDLTSDPRP 1260

Query: 1261 EVRSCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRET 1320
            EVRSCALEVLFDLLNERG KFSTSFWESIFHRVLFPIFDHLR  GKE++ +SGDEWLRET
Sbjct: 1261 EVRSCALEVLFDLLNERGDKFSTSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRET 1320

Query: 1321 SIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQF 1380
            SIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQF
Sbjct: 1321 SIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQF 1380

Query: 1381 SEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFD 1440
            SEDDWDTLLKSIRDASYTTQPLELLNALGFENPSH E+NIVD  SLK  SQQEAKNH  D
Sbjct: 1381 SEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHID 1440

Query: 1441 VGDHGKASPVASPR-GESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQ 1500
            V DHGK SPV SPR  E ITR+PI ESGLQIT+DE AEG+PSPSTRAT+AAE G+LQRSQ
Sbjct: 1441 VSDHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAGNLQRSQ 1500

Query: 1501 TIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIV 1560
            TIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPI+LPPD +DPEVK+DEES L+ IV
Sbjct: 1501 TIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDAVDPEVKDDEESPLLGIV 1560

Query: 1561 RGKCITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHI 1620
            RGKCITQLLLLGVIDGIQKKYW KLNAPQKIAIMDILLSLLEF+ATYNSYNNLRQRM+HI
Sbjct: 1561 RGKCITQLLLLGVIDGIQKKYWVKLNAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHI 1620

Query: 1621 PDE-RPPLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSS 1680
            PDE RPPLNLLRQELAGTS+YLDILLKATS FN++EAE++K VD+LEV++ SP  DLTS+
Sbjct: 1621 PDESRPPLNLLRQELAGTSIYLDILLKATSGFNSIEAEQKKIVDSLEVDSESPKDDLTST 1680

Query: 1681 PDSSAAKNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKG 1740
             DSSA  NVD IAENRLVSFCEQALRE SDL SSA E THMDVHRVLELRSPVIVKVIKG
Sbjct: 1681 QDSSAVNNVDRIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKG 1740

Query: 1741 MCFMNSEIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1780
            MCFMNS+IFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL
Sbjct: 1741 MCFMNSQIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1785

BLAST of MC05g0262 vs. ExPASy TrEMBL
Match: A0A0A0L3W2 (SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G126980 PE=4 SV=1)

HSP 1 Score: 3124 bits (8100), Expect = 0.0
Identity = 1614/1784 (90.47%), Postives = 1694/1784 (94.96%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGFV RAFESMLKECS GKKYPALQKAIQA+LD+TKE N++Q +TP ET+QPA SAG
Sbjct: 1    MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDATKEVNRSQQATPIETNQPAASAG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
            DTSETGGE DE++T + +A+E EN G+KAAP+E IS+VLANAGHVL G DAE+VL+PLRL
Sbjct: 61   DTSETGGEADESQTAQ-SAQEVENNGKKAAPREHISIVLANAGHVLHGDDAELVLSPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AFDTK+LKV+EL+LDCLHKLIAYDHLEGDPGLEGGKNV +FTDILNMICGC+DNSS DST
Sbjct: 121  AFDTKHLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVSLFTDILNMICGCIDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISIIFR
Sbjct: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETDQVSLSTSSGTK+S SAE SS V EET V +ENDKETTLGDALNSVKDTS+ASVEE
Sbjct: 241  RMETDQVSLSTSSGTKDSSSAEVSSVVDEETTVNEENDKETTLGDALNSVKDTSIASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            LQNLAGGADIKGLEAVLDKAVH EDGKKMSRGIDLESVNI++RDALLVFRT+CKMGMKED
Sbjct: 301  LQNLAGGADIKGLEAVLDKAVHIEDGKKMSRGIDLESVNIIQRDALLVFRTLCKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
             DEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQ PVIFQYA
Sbjct: 361  TDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQPPVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQK SVLKMLEKICREPQILVD
Sbjct: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKTSVLKMLEKICREPQILVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            IFVNYDCDLEAPNLFERMVTTLSK+SQGTQNADPN+ A+SQ TSIKGSSLQCLVNVLKSL
Sbjct: 481  IFVNYDCDLEAPNLFERMVTTLSKLSQGTQNADPNLAALSQATSIKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEKSRL S+K+   V S EEESSG ENLE+KSRED+T NFEKAKAHKSTVEAAISEFN
Sbjct: 541  VDWEKSRLHSEKEG-LVHSSEEESSGNENLEVKSREDVTGNFEKAKAHKSTVEAAISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            RKPVKGVEYLISNKLVENTPSSVA FLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM
Sbjct: 601  RKPVKGVEYLISNKLVENTPSSVALFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            +FSGMKFDAAIR+FLKGFRLPGEAQKIDRIMEKFAERYCA NPGLFKNADTAYVLAYAVI
Sbjct: 661  KFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRG 780
            MLNTDAHNPMVWPKMS+ DF RMNV NDPE+CAPTELLE+IYDSIVKEEIKMKDD  D+ 
Sbjct: 721  MLNTDAHNPMVWPKMSKSDFTRMNVMNDPEDCAPTELLEEIYDSIVKEEIKMKDDLLDKA 780

Query: 781  KNRR-EGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQR 840
            K+RR E EE+GGLVSILNLALPRR+SST+A+SESEAI+KQTQVIFRNQG KRG+FYTSQR
Sbjct: 781  KSRRLEVEEKGGLVSILNLALPRRKSSTEAQSESEAIIKQTQVIFRNQGAKRGVFYTSQR 840

Query: 841  IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT 900
            IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT
Sbjct: 841  IELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLT 900

Query: 901  SLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSIA 960
            SLVRFTFLHAPKEMRSKNVEALRTLL LCDLETESLQDTWNAVLECVSRLEFITSTPSIA
Sbjct: 901  SLVRFTFLHAPKEMRSKNVEALRTLLALCDLETESLQDTWNAVLECVSRLEFITSTPSIA 960

Query: 961  STVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT 1020
            +TVMYGSNQISRDAVVQSL+EL+GKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT
Sbjct: 961  ATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQT 1020

Query: 1021 PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM 1080
            PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM
Sbjct: 1021 PARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLGM 1080

Query: 1081 KYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSVF 1140
            KYLERAELANFTFQNDILKPFVVLMRNS SESIRSLIVDCIVQMIKSKVGNIKSGWRSVF
Sbjct: 1081 KYLERAELANFTFQNDILKPFVVLMRNSQSESIRSLIVDCIVQMIKSKVGNIKSGWRSVF 1140

Query: 1141 MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK 1200
            MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK
Sbjct: 1141 MIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISLK 1200

Query: 1201 AIALLRICEDRLAEGLIPGGALKPIHD--SADPAFDVTEHYWFPMLAGLSDLTSDSRPEV 1260
            AIALLRICEDRLAEGLIPGGALKPIHD  SA+PAFD+TEHYWFPMLAGLSDLTSD RPEV
Sbjct: 1201 AIALLRICEDRLAEGLIPGGALKPIHDNESAEPAFDMTEHYWFPMLAGLSDLTSDPRPEV 1260

Query: 1261 RSCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSI 1320
            RSCALEVLFDLLNERG+KFS SFWESIFHRVLFPIFDHLR  GKE++ +SGDEWLRETSI
Sbjct: 1261 RSCALEVLFDLLNERGSKFSMSFWESIFHRVLFPIFDHLRHAGKESVNSSGDEWLRETSI 1320

Query: 1321 HSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSE 1380
            HSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSE
Sbjct: 1321 HSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSE 1380

Query: 1381 DDWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVG 1440
            DDWDTLLKSIRDASYTTQPLELLNALGFENPSH E+NIVD  SLK  SQQEAKNH  DV 
Sbjct: 1381 DDWDTLLKSIRDASYTTQPLELLNALGFENPSHDELNIVDDGSLKWSSQQEAKNHHIDVN 1440

Query: 1441 DHGKASPVASPR-GESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTI 1500
            +HGK SPV SPR  E ITR+PI ESGLQIT+DE AEG+PSPSTRAT+AAE  +LQRSQTI
Sbjct: 1441 EHGKVSPVPSPRVAEIITRSPIAESGLQITTDESAEGIPSPSTRATRAAEAANLQRSQTI 1500

Query: 1501 GQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRG 1560
            GQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPI+LPPDT+DPEVK+DEES L+ IVRG
Sbjct: 1501 GQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIRLPPDTVDPEVKDDEESPLLGIVRG 1560

Query: 1561 KCITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPD 1620
            KCITQLLLLGVIDGIQKKYW KL+APQKIAIMDILLSLLEF+ATYNSYNNLRQRM+HIPD
Sbjct: 1561 KCITQLLLLGVIDGIQKKYWVKLSAPQKIAIMDILLSLLEFSATYNSYNNLRQRMNHIPD 1620

Query: 1621 ERPPLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDS 1680
            ERPPLNLLRQELAGTS+YLDILLKATS FNT+EAE++K  D+LEV++ SP  DLTS  DS
Sbjct: 1621 ERPPLNLLRQELAGTSIYLDILLKATSGFNTIEAEQEKIADSLEVDSESPKDDLTSIQDS 1680

Query: 1681 SAAKNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCF 1740
            SA  NVDGIAENRLVSFCEQALRE SDL SSA E THMDVHRVLELRSPVIVKVIKGMCF
Sbjct: 1681 SAVSNVDGIAENRLVSFCEQALREVSDLQSSAVETTHMDVHRVLELRSPVIVKVIKGMCF 1740

Query: 1741 MNSEIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1780
            MNS+IFRRHLREFYPLLTKLVCCDQID+RGALGDLFKIQLKALL
Sbjct: 1741 MNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGDLFKIQLKALL 1782

BLAST of MC05g0262 vs. ExPASy TrEMBL
Match: A0A6J1F568 (brefeldin A-inhibited guanine nucleotide-exchange protein 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442227 PE=4 SV=1)

HSP 1 Score: 3121 bits (8091), Expect = 0.0
Identity = 1610/1792 (89.84%), Postives = 1691/1792 (94.36%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGFV RAFESMLKECS GKKYPALQKAIQA+LDSTKE NQ+QHSTP ET QP TSAG
Sbjct: 1    MAAGGFVTRAFESMLKECSGGKKYPALQKAIQAFLDSTKEVNQSQHSTPVETIQPETSAG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
            DTSETGGE DE+ T + TA+EAEN G K AP+E IS+VLANAGHVL+GADAEIVLNPLRL
Sbjct: 61   DTSETGGEADESPTAQ-TAQEAENEGSKVAPREHISIVLANAGHVLQGADAEIVLNPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AFDTKNLKV+EL+LDCLHKLIAYDHLEGDPGLEGGKNVP+FTDILNMICGC+DNSS DST
Sbjct: 121  AFDTKNLKVLELALDCLHKLIAYDHLEGDPGLEGGKNVPLFTDILNMICGCIDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISIIFR
Sbjct: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIIFR 240

Query: 241  RMETDQVSLSTS-----------SGTKESISAETSSKVVEETAVTDENDKETTLGDALNS 300
            RMETDQVSLSTS           SGTKESISAE SS V EE  V+DEN  ETTLGDALNS
Sbjct: 241  RMETDQVSLSTSTGTKESISAEISGTKESISAEISSGVDEENTVSDENGNETTLGDALNS 300

Query: 301  VKDTSLASVEELQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVF 360
            VKDTSLASVEELQNLAGGADIKGLEAVLDKAVH+EDGKKMSRGIDLESVNIV+RDALLVF
Sbjct: 301  VKDTSLASVEELQNLAGGADIKGLEAVLDKAVHSEDGKKMSRGIDLESVNIVQRDALLVF 360

Query: 361  RTICKMGMKEDADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRAS 420
            RT+CKMGMKED DEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRAS
Sbjct: 361  RTLCKMGMKEDTDEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRAS 420

Query: 421  VSQSPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLE 480
            VSQSPVIFQYATGIFSVLLLRFR+SLKGEIGIFFPLIVLRSLDGTDFPVNQK+SVLKMLE
Sbjct: 421  VSQSPVIFQYATGIFSVLLLRFRDSLKGEIGIFFPLIVLRSLDGTDFPVNQKLSVLKMLE 480

Query: 481  KICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSS 540
            KICREPQILVDIFVNYDCDLEAPNLFER VTTLS+ISQGTQNADPN+ AVSQTTSIKGSS
Sbjct: 481  KICREPQILVDIFVNYDCDLEAPNLFERTVTTLSRISQGTQNADPNLAAVSQTTSIKGSS 540

Query: 541  LQCLVNVLKSLVDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHK 600
            LQCLVNVLKSLVDWEKSRL S+KQS ++ S EEE SG ENLEIKSRED+T NFEKAKAHK
Sbjct: 541  LQCLVNVLKSLVDWEKSRLHSEKQSLSIHSSEEEPSGNENLEIKSREDVTGNFEKAKAHK 600

Query: 601  STVEAAISEFNRKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFP 660
            STVEA ISEFNRKPVKGVEYLI+NKLV NTPSSVAQFL+NTPSLDKTMIG+YLGQHEEFP
Sbjct: 601  STVEAVISEFNRKPVKGVEYLIANKLVANTPSSVAQFLQNTPSLDKTMIGEYLGQHEEFP 660

Query: 661  VAVMHAYVDSMQFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNA 720
            +AVMHAYVD M+FSGMKFDAAIR+FLKGFRLPGEAQKIDRIMEKFAERYCA NPGLFKNA
Sbjct: 661  IAVMHAYVDCMKFSGMKFDAAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNA 720

Query: 721  DTAYVLAYAVIMLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEE 780
            DTAYVLAYAVIMLNTDAHNPMVWPKMS+PDF+RMNVTNDPE+CAPTELLE+IYDSIVKEE
Sbjct: 721  DTAYVLAYAVIMLNTDAHNPMVWPKMSKPDFVRMNVTNDPEDCAPTELLEEIYDSIVKEE 780

Query: 781  IKMKDDTSDRGKNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGG 840
            IKMKDDTSD+ K+RREGEERGGLVSILNLALP+R+SSTDAKSESEAI+KQTQVIFRNQG 
Sbjct: 781  IKMKDDTSDKAKSRREGEERGGLVSILNLALPKRKSSTDAKSESEAIIKQTQVIFRNQGA 840

Query: 841  KRGIFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLG 900
            KRG+FYTS+RIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHIT+VLG
Sbjct: 841  KRGVFYTSKRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITYVLG 900

Query: 901  MDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRL 960
            MDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLL LCDLETESLQDTWNAVLEC SRL
Sbjct: 901  MDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLDLCDLETESLQDTWNAVLECASRL 960

Query: 961  EFITSTPSIASTVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALC 1020
            EFITSTPSIA+TVMYGSNQISRDAVVQSL+EL+GKPADQVFVNSV+LPSDSVVEFFTALC
Sbjct: 961  EFITSTPSIAATVMYGSNQISRDAVVQSLRELAGKPADQVFVNSVRLPSDSVVEFFTALC 1020

Query: 1021 GVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMY 1080
            GVSAEELKQTPARVFSLQKLVEISYYNMARIR+VWARIWSVLSNHFI+AGSHHDEKI+MY
Sbjct: 1021 GVSAEELKQTPARVFSLQKLVEISYYNMARIRLVWARIWSVLSNHFIAAGSHHDEKISMY 1080

Query: 1081 AIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVG 1140
            AIDSLRQLGMKYLERAELANFTFQNDILKPFV+LMRNS SESIRSLIVDCIVQMIKSKVG
Sbjct: 1081 AIDSLRQLGMKYLERAELANFTFQNDILKPFVILMRNSQSESIRSLIVDCIVQMIKSKVG 1140

Query: 1141 NIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFAN 1200
            NIKSGWRSVFMIFTASADDESE+IVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFAN
Sbjct: 1141 NIKSGWRSVFMIFTASADDESETIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFAN 1200

Query: 1201 NKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDL 1260
            NKS HRISLKAIALLRICEDRLAEGLIPGGALKPIHDS DPAFD+TEHYWFPMLAGLSDL
Sbjct: 1201 NKSFHRISLKAIALLRICEDRLAEGLIPGGALKPIHDSTDPAFDMTEHYWFPMLAGLSDL 1260

Query: 1261 TSDSRPEVRSCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGD 1320
            TSD RPEVRSCALEVLFDLLNERGAKFSTSFWE+IFH VLFPIF HLR  GKE+L +SGD
Sbjct: 1261 TSDPRPEVRSCALEVLFDLLNERGAKFSTSFWENIFHHVLFPIFHHLRHVGKESLNSSGD 1320

Query: 1321 EWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIE 1380
            EWLRETSIHS+QLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIE
Sbjct: 1321 EWLRETSIHSIQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIE 1380

Query: 1381 VGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEA 1440
            VGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSH EVN+V G SLKSVS  EA
Sbjct: 1381 VGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHDEVNVVGGTSLKSVSPLEA 1440

Query: 1441 KNHQFDVGDHGKASPVASPRGESI-TRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGG 1500
            KN  FDV DH K SP+ SPR   I TRN I ESG+QITSDE AEGLPSPSTRA   AE G
Sbjct: 1441 KNLHFDVSDHRKVSPLPSPRVAEINTRNTINESGIQITSDESAEGLPSPSTRA---AEAG 1500

Query: 1501 SLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEES 1560
            SLQRSQTIGQRIMGNMMDNIFVRSLTSKSKG ASDASVPSSPIKLPPDT+DPEVK++EE 
Sbjct: 1501 SLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGVASDASVPSSPIKLPPDTVDPEVKDEEEI 1560

Query: 1561 SLMAIVRGKCITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLR 1620
             L+ IVRGKC+TQLLLLGVIDGIQKKYW KLNAPQKIAIMDILLSLLEF+ATYNSYNNLR
Sbjct: 1561 PLLGIVRGKCVTQLLLLGVIDGIQKKYWVKLNAPQKIAIMDILLSLLEFSATYNSYNNLR 1620

Query: 1621 QRMHHIPDERPPLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNG 1680
            QRMHHIP+ERPPLNLLRQELAGTS+YLDILLKATS  NT+EA ++K VD LEV+T S N 
Sbjct: 1621 QRMHHIPNERPPLNLLRQELAGTSIYLDILLKATSGINTIEAGQEKTVDKLEVKTESAND 1680

Query: 1681 DLTSSPDSSAAKNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIV 1740
             LTS+PDSSA  +VDG+AE+RLVSFCE ALREASDL SS GEATHMDVH+VLELRSPVIV
Sbjct: 1681 GLTSAPDSSAVNDVDGVAESRLVSFCEHALREASDLQSSVGEATHMDVHQVLELRSPVIV 1740

Query: 1741 KVIKGMCFMNSEIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1780
            KVIKGMCFMNS+IFRRHLREFYPLLTKLVCCDQID+RGALG+LFKIQLKAL+
Sbjct: 1741 KVIKGMCFMNSQIFRRHLREFYPLLTKLVCCDQIDIRGALGELFKIQLKALV 1788

BLAST of MC05g0262 vs. TAIR 10
Match: AT3G43300.1 (HOPM interactor 7 )

HSP 1 Score: 2474.5 bits (6412), Expect = 0.0e+00
Identity = 1299/1801 (72.13%), Postives = 1488/1801 (82.62%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGF+ RAF++MLKE S GKK+P LQKAIQAY D +K   Q   S+  E+SQ   + G
Sbjct: 1    MAAGGFLTRAFDTMLKE-SGGKKFPDLQKAIQAYQDGSKVVTQAAPSSIVESSQ---AEG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
               +TG E DE + V  +AE A+   +  +  E I+V LANAGH L GA+ E+VL PLRL
Sbjct: 61   GGEKTGVEADEPQKV-TSAEVAQQASQ--SKSETINVSLANAGHTLGGAEVELVLKPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AF+TKNLK+ + +LDCLHKLIAYDHLEGDPGL+GGKN   FTDILNM+C CVDNSS DST
Sbjct: 121  AFETKNLKIFDAALDCLHKLIAYDHLEGDPGLDGGKNSAPFTDILNMVCSCVDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            +LQVLKVLLTAVAS KF+VHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISI+FR
Sbjct: 181  VLQVLKVLLTAVASGKFKVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIVFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETD VS S++   +E +S +TSS   EE    DEN+KE TLGDAL   KDT+LASVEE
Sbjct: 241  RMETDIVSASSTVSQEEHVSGDTSSPKNEEITAADENEKEMTLGDALTQAKDTTLASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            L  L GGADIKGLEA LDKAVH EDGKK+ RGI+LES++I +RDALLVFRT+CKMGMKED
Sbjct: 301  LHTLVGGADIKGLEAALDKAVHLEDGKKIKRGIELESMSIGQRDALLVFRTLCKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
            +DEVTTKTRILSLELLQG+LEGVS +FTK+FHFIDSVKAYLSYALLRASVSQS VIFQYA
Sbjct: 361  SDEVTTKTRILSLELLQGMLEGVSHSFTKNFHFIDSVKAYLSYALLRASVSQSSVIFQYA 420

Query: 421  TGIFSVLLLRFRESLK-------------------GEIGIFFPLIVLRSLDGTDFPVNQK 480
            +GIFSVLLLRFR+SLK                   GEIGIFFP+IVLRSLD ++ P +QK
Sbjct: 421  SGIFSVLLLRFRDSLKVSMDCYLSPYFSDPKSHSQGEIGIFFPIIVLRSLDNSECPNDQK 480

Query: 481  ISVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQ 540
            + VL+MLEK+C++PQ+LVD++VNYDCDLEAPNLFERMVTTLSKI+QG+Q+ADPN    SQ
Sbjct: 481  MGVLRMLEKVCKDPQMLVDVYVNYDCDLEAPNLFERMVTTLSKIAQGSQSADPNPAMASQ 540

Query: 541  TTSIKGSSLQCLVNVLKSLVDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSN 600
            T S+KGSSLQCLVNVLKSLVDWEK R +++  + N    E+ +S  E +E KSRED+ SN
Sbjct: 541  TASVKGSSLQCLVNVLKSLVDWEKIRREAENSTRNAN--EDSASTGEPIETKSREDVPSN 600

Query: 601  FEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDY 660
            FEKAKAHKST+EAAISEFNR  VKGVEYLI+NKLVE  P+SVAQFLR+T SL K MIGDY
Sbjct: 601  FEKAKAHKSTMEAAISEFNRNSVKGVEYLIANKLVERNPASVAQFLRSTSSLSKVMIGDY 660

Query: 661  LGQHEEFPVAVMHAYVDSMQFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAG 720
            LGQHEEFP+AVMHAYVDSM+FS MKF +AIR+FLKGFRLPGEAQKIDRIMEKFAERYCA 
Sbjct: 661  LGQHEEFPLAVMHAYVDSMKFSEMKFHSAIREFLKGFRLPGEAQKIDRIMEKFAERYCAD 720

Query: 721  NPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDI 780
            NPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMS+ DF RMN TNDPE+CAPTELLE+I
Sbjct: 721  NPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNATNDPEDCAPTELLEEI 780

Query: 781  YDSIVKEEIKMKDDTSDR--GKNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQ 840
            YDSIV+EEIK+KDD + +     R  GEERGGLVSILNL LP+R S+ DAKSE+E IV++
Sbjct: 781  YDSIVQEEIKLKDDDTMKKLSSQRPGGEERGGLVSILNLGLPKRISAADAKSETEDIVRK 840

Query: 841  TQVIFRNQGGKRGIFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFR 900
            TQ IFR  G KRG+F+T ++++++RPMVEAVGWPLLA FSVTME GDNKPR++LCMEGF+
Sbjct: 841  TQEIFRKHGVKRGVFHTVEQVDIIRPMVEAVGWPLLAAFSVTMEVGDNKPRILLCMEGFK 900

Query: 901  AGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTW 960
            AGIHI +VLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALR LL LCD E ++LQDTW
Sbjct: 901  AGIHIAYVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRILLGLCDSEPDTLQDTW 960

Query: 961  NAVLECVSRLEFITSTPSIASTVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSD 1020
            NAVLECVSRLEFI STP IA+TVM+GSNQISRD VVQSLKEL+G+PA+QVFVNSVKLPS+
Sbjct: 961  NAVLECVSRLEFIISTPGIAATVMHGSNQISRDGVVQSLKELAGRPAEQVFVNSVKLPSE 1020

Query: 1021 SVVEFFTALCGVSAEELKQTPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAG 1080
            SVVEFFTALCGVSAEELKQ+PARVFSLQKLVEISYYN+ARIRMVWARIWSVL+ HF+SAG
Sbjct: 1021 SVVEFFTALCGVSAEELKQSPARVFSLQKLVEISYYNIARIRMVWARIWSVLAEHFVSAG 1080

Query: 1081 SHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDC 1140
            SHHDEKIAMYAIDSLRQLGMKYLERAEL NFTFQNDILKPFV++MRN+ S++IRSLIVDC
Sbjct: 1081 SHHDEKIAMYAIDSLRQLGMKYLERAELTNFTFQNDILKPFVIIMRNTQSQTIRSLIVDC 1140

Query: 1141 IVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMD 1200
            IVQMIKSKVG+IKSGWRSVFMIFTA+ADDE ESIVE +FENVEQVILEHFDQV+GDCFMD
Sbjct: 1141 IVQMIKSKVGSIKSGWRSVFMIFTAAADDEVESIVEKSFENVEQVILEHFDQVIGDCFMD 1200

Query: 1201 CVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYW 1260
            CVNCLIRFANNK+S RISLKAIALLRICEDRLAEGLIPGG LKP+  + D  FDVTEHYW
Sbjct: 1201 CVNCLIRFANNKASDRISLKAIALLRICEDRLAEGLIPGGVLKPVDGNEDETFDVTEHYW 1260

Query: 1261 FPMLAGLSDLTSDSRPEVRSCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQP 1320
            FPMLAGLSDLTSD RPEVR+CALEVLFDLLNERG KFST FWESIFHR+LFPIFDH+   
Sbjct: 1261 FPMLAGLSDLTSDYRPEVRNCALEVLFDLLNERGNKFSTPFWESIFHRILFPIFDHVSHA 1320

Query: 1321 GKENLITSGDEWLRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSL 1380
            GKE+LI+SGD   RETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAK+ +Q+VVS+
Sbjct: 1321 GKESLISSGDVKFRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKKSDQTVVSI 1380

Query: 1381 ALGALVHLIEVGGHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGI 1440
            +LGALVHLIEVGGHQFSE DWD LLKSIRDASYTTQPLELLNAL F+NP    V +   I
Sbjct: 1381 SLGALVHLIEVGGHQFSEGDWDMLLKSIRDASYTTQPLELLNALSFDNPKKNLV-LAGDI 1440

Query: 1441 SLKSVSQQEAKNHQFDVGDHGKASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPST 1500
               +        +  D+ D+GK S  ASPR    T     ESG+   +D  +EG PS S 
Sbjct: 1441 EADASDSPRVDRNPDDIKDNGKVSAQASPR--IGTHGTSLESGIPPKADG-SEGRPSSSG 1500

Query: 1501 RATKAAEGGSLQRSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLD 1560
            RA K  +  +LQRSQT GQR     MDN+F+R+LTS+ K   ++ +VPSSP K   D  +
Sbjct: 1501 RAQKDVDDVNLQRSQTFGQR----FMDNLFLRNLTSQPKSSVAEVTVPSSPYK-HEDPTE 1560

Query: 1561 PEVKEDEESSLMAIVRGKCITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAA 1620
            P+ +E EES  +  +RGKCITQLLLLG I+ IQ+KYW  L  PQKIAIMDIL S +EFA+
Sbjct: 1561 PDSRE-EESPALGAIRGKCITQLLLLGAINSIQQKYWSNLKTPQKIAIMDILFSFIEFAS 1620

Query: 1621 TYNSYNNLRQRMHHIPDERPPLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNL 1680
            +YNSY+NLR RM+HIP ERPPLNLLRQEL GT++YLD+L K TS                
Sbjct: 1621 SYNSYSNLRTRMNHIPTERPPLNLLRQELEGTTIYLDVLQKTTSGL-------------- 1680

Query: 1681 EVETASPNGDLTSSPDSSAAKNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRV 1740
                        +   S++   ++G AE +LVSFCEQ L+E SDL S+ GE T+MDVHRV
Sbjct: 1681 ------------ADDASNSEDRLEGAAEEKLVSFCEQVLKETSDLQSTLGETTNMDVHRV 1740

Query: 1741 LELRSPVIVKVIKGMCFMNSEIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKAL 1781
            LELRSPVIVKV++GMCFMN+ IFR+H+REFYPLLT+LVCC+Q+++RGAL +LFK QLK L
Sbjct: 1741 LELRSPVIVKVLEGMCFMNNTIFRKHMREFYPLLTRLVCCEQMEIRGALANLFKAQLKPL 1756

BLAST of MC05g0262 vs. TAIR 10
Match: AT3G43300.2 (HOPM interactor 7 )

HSP 1 Score: 2428.3 bits (6292), Expect = 0.0e+00
Identity = 1280/1782 (71.83%), Postives = 1469/1782 (82.44%), Query Frame = 0

Query: 1    MAAGGFVGRAFESMLKECSSGKKYPALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAG 60
            MAAGGF+ RAF++MLKE S GKK+P LQKAIQAY D +K   Q   S+  E+SQ   + G
Sbjct: 1    MAAGGFLTRAFDTMLKE-SGGKKFPDLQKAIQAYQDGSKVVTQAAPSSIVESSQ---AEG 60

Query: 61   DTSETGGEVDEARTVEETAEEAENVGRKAAPQEPISVVLANAGHVLEGADAEIVLNPLRL 120
               +TG E DE + V  +AE A+   +  +  E I+V LANAGH L GA+ E+VL PLRL
Sbjct: 61   GGEKTGVEADEPQKV-TSAEVAQQASQ--SKSETINVSLANAGHTLGGAEVELVLKPLRL 120

Query: 121  AFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDST 180
            AF+TKNLK+ + +LDCLHKLIAYDHLEGDPGL+GGKN   FTDILNM+C CVDNSS DST
Sbjct: 121  AFETKNLKIFDAALDCLHKLIAYDHLEGDPGLDGGKNSAPFTDILNMVCSCVDNSSPDST 180

Query: 181  ILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFR 240
            +LQVLKVLLTAVAS KF+VHGEPLLGVIRVCYNIAL+SKSPINQATSKAMLTQMISI+FR
Sbjct: 181  VLQVLKVLLTAVASGKFKVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIVFR 240

Query: 241  RMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGDALNSVKDTSLASVEE 300
            RMETD VS S++   +E +S +TSS   EE    DEN+KE TLGDAL   KDT+LASVEE
Sbjct: 241  RMETDIVSASSTVSQEEHVSGDTSSPKNEEITAADENEKEMTLGDALTQAKDTTLASVEE 300

Query: 301  LQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMKED 360
            L  L GGADIKGLEA LDKAVH EDGKK+ RGI+LES++I +RDALLVFRT+CKMGMKED
Sbjct: 301  LHTLVGGADIKGLEAALDKAVHLEDGKKIKRGIELESMSIGQRDALLVFRTLCKMGMKED 360

Query: 361  ADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYA 420
            +DEVTTKTRILSLELLQG+LEGVS +FTK+FHFIDSVKAYLSYALLRASVSQS VIFQYA
Sbjct: 361  SDEVTTKTRILSLELLQGMLEGVSHSFTKNFHFIDSVKAYLSYALLRASVSQSSVIFQYA 420

Query: 421  TGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFPVNQKISVLKMLEKICREPQILVD 480
            +GIFSVLLLRFR+SLKGEIGIFFP+IVLRSLD ++ P +QK+ VL+MLEK+C++PQ+LVD
Sbjct: 421  SGIFSVLLLRFRDSLKGEIGIFFPIIVLRSLDNSECPNDQKMGVLRMLEKVCKDPQMLVD 480

Query: 481  IFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSL 540
            ++VNYDCDLEAPNLFERMVTTLSKI+QG+Q+ADPN    SQT S+KGSSLQCLVNVLKSL
Sbjct: 481  VYVNYDCDLEAPNLFERMVTTLSKIAQGSQSADPNPAMASQTASVKGSSLQCLVNVLKSL 540

Query: 541  VDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLTSNFEKAKAHKSTVEAAISEFN 600
            VDWEK R +++  + N    E+ +S  E +E KSRED+ SNFEKAKAHKST+EAAISEFN
Sbjct: 541  VDWEKIRREAENSTRNAN--EDSASTGEPIETKSREDVPSNFEKAKAHKSTMEAAISEFN 600

Query: 601  RKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSM 660
            R  VKGVEYLI+NKLVE  P+SVAQFLR+T SL K MIGDYLGQHEEFP+AVMHAYVDSM
Sbjct: 601  RNSVKGVEYLIANKLVERNPASVAQFLRSTSSLSKVMIGDYLGQHEEFPLAVMHAYVDSM 660

Query: 661  QFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVI 720
            +FS MKF +AIR+FLKGFRLPGEAQKIDRIMEKFAERYCA NPGLFKNADTAYVLAYAVI
Sbjct: 661  KFSEMKFHSAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVI 720

Query: 721  MLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDR- 780
            MLNTDAHNPMVWPKMS+ DF RMN TNDPE+CAPTELLE+IYDSIV+EEIK+KDD + + 
Sbjct: 721  MLNTDAHNPMVWPKMSKSDFTRMNATNDPEDCAPTELLEEIYDSIVQEEIKLKDDDTMKK 780

Query: 781  -GKNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQGGKRGIFYTSQ 840
                R  GEERGGLVSILNL LP+R S+ DAKSE+E IV++TQ IFR  G KRG+F+T +
Sbjct: 781  LSSQRPGGEERGGLVSILNLGLPKRISAADAKSETEDIVRKTQEIFRKHGVKRGVFHTVE 840

Query: 841  RIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFL 900
            +++++RPMVEAVGWPLLA FSVTME GDNKPR++LCMEGF+AGIHI +VLGMDTMRYAFL
Sbjct: 841  QVDIIRPMVEAVGWPLLAAFSVTMEVGDNKPRILLCMEGFKAGIHIAYVLGMDTMRYAFL 900

Query: 901  TSLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECVSRLEFITSTPSI 960
            TSLVRFTFLHAPKEMRSKNVEALR LL LCD E ++LQDTWNAVLECVSRLEFI STP I
Sbjct: 901  TSLVRFTFLHAPKEMRSKNVEALRILLGLCDSEPDTLQDTWNAVLECVSRLEFIISTPGI 960

Query: 961  ASTVMYGSNQISRDAVVQSLKELSGKPADQVFVNSVKLPSDSVVEFFTALCGVSAEELKQ 1020
            A+TVM+GSNQISRD VVQSLKEL+G+PA+QVFVNSVKLPS+SVVEFFTALCGVSAEELKQ
Sbjct: 961  AATVMHGSNQISRDGVVQSLKELAGRPAEQVFVNSVKLPSESVVEFFTALCGVSAEELKQ 1020

Query: 1021 TPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYAIDSLRQLG 1080
            +PARVFSLQKLVEISYYN+ARIRMVWARIWSVL+ HF+SAGSHHDEKIAMYAIDSLRQLG
Sbjct: 1021 SPARVFSLQKLVEISYYNIARIRMVWARIWSVLAEHFVSAGSHHDEKIAMYAIDSLRQLG 1080

Query: 1081 MKYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGNIKSGWRSV 1140
            MKYLERAEL NFTFQNDILKPFV++MRN+ S++IRSLIVDCIVQMIKSKVG+IKSGWRSV
Sbjct: 1081 MKYLERAELTNFTFQNDILKPFVIIMRNTQSQTIRSLIVDCIVQMIKSKVGSIKSGWRSV 1140

Query: 1141 FMIFTASADDESESIVESAFENVEQVILEHFDQVVGDCFMDCVNCLIRFANNKSSHRISL 1200
            FMIFTA+ADDE ESIVE +FENVEQ          GD     +  L  FANNK+S RISL
Sbjct: 1141 FMIFTAAADDEVESIVEKSFENVEQ----------GD--KQSIKLLHLFANNKASDRISL 1200

Query: 1201 KAIALLRICEDRLAEGLIPGGALKPIHDSADPAFDVTEHYWFPMLAGLSDLTSDSRPEVR 1260
            KAIALLRICEDRLAEGLIPGG LKP+  + D  FDVTEHYWFPMLAGLSDLTSD RPEVR
Sbjct: 1201 KAIALLRICEDRLAEGLIPGGVLKPVDGNEDETFDVTEHYWFPMLAGLSDLTSDYRPEVR 1260

Query: 1261 SCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFDHLRQPGKENLITSGDEWLRETSIH 1320
            +CALEVLFDLLNERG KFST FWESIFHR+LFPIFDH+   GKE+LI+SGD   RETSIH
Sbjct: 1261 NCALEVLFDLLNERGNKFSTPFWESIFHRILFPIFDHVSHAGKESLISSGDVKFRETSIH 1320

Query: 1321 SLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVGGHQFSED 1380
            SLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAK+ +Q+VVS++LGALVHLIEVGGHQFSE 
Sbjct: 1321 SLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKKSDQTVVSISLGALVHLIEVGGHQFSEG 1380

Query: 1381 DWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKNHQFDVGD 1440
            DWD LLKSIRDASYTTQPLELLNAL F+NP    V +   I   +        +  D+ D
Sbjct: 1381 DWDMLLKSIRDASYTTQPLELLNALSFDNPKKNLV-LAGDIEADASDSPRVDRNPDDIKD 1440

Query: 1441 HGKASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQRSQTIGQ 1500
            +GK S  ASPR    T     ESG+   +D  +EG PS S RA K  +  +LQRSQT GQ
Sbjct: 1441 NGKVSAQASPR--IGTHGTSLESGIPPKADG-SEGRPSSSGRAQKDVDDVNLQRSQTFGQ 1500

Query: 1501 RIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLMAIVRGKC 1560
            R     MDN+F+R+LTS+ K   ++ +VPSSP K   D  +P+ +E EES  +  +RGKC
Sbjct: 1501 R----FMDNLFLRNLTSQPKSSVAEVTVPSSPYK-HEDPTEPDSRE-EESPALGAIRGKC 1560

Query: 1561 ITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRMHHIPDER 1620
            ITQLLLLG I+ IQ+KYW  L  PQKIAIMDIL S +EFA++YNSY+NLR RM+HIP ER
Sbjct: 1561 ITQLLLLGAINSIQQKYWSNLKTPQKIAIMDILFSFIEFASSYNSYSNLRTRMNHIPTER 1620

Query: 1621 PPLNLLRQELAGTSVYLDILLKATSRFNTLEAEEQKFVDNLEVETASPNGDLTSSPDSSA 1680
            PPLNLLRQEL GT++YLD+L K TS                            +   S++
Sbjct: 1621 PPLNLLRQELEGTTIYLDVLQKTTSGL--------------------------ADDASNS 1680

Query: 1681 AKNVDGIAENRLVSFCEQALREASDLHSSAGEATHMDVHRVLELRSPVIVKVIKGMCFMN 1740
               ++G AE +LVSFCEQ L+E SDL S+ GE T+MDVHRVLELRSPVIVKV++GMCFMN
Sbjct: 1681 EDRLEGAAEEKLVSFCEQVLKETSDLQSTLGETTNMDVHRVLELRSPVIVKVLEGMCFMN 1725

Query: 1741 SEIFRRHLREFYPLLTKLVCCDQIDVRGALGDLFKIQLKALL 1781
            + IFR+H+REFYPLLT+LVCC+Q+++RGAL +LFK QLK LL
Sbjct: 1741 NTIFRKHMREFYPLLTRLVCCEQMEIRGALANLFKAQLKPLL 1725

BLAST of MC05g0262 vs. TAIR 10
Match: AT1G01960.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 894.0 bits (2309), Expect = 1.9e-259
Identity = 556/1433 (38.80%), Postives = 809/1433 (56.45%), Query Frame = 0

Query: 109  ADAEIVLNPLRLAFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDILNMI 168
            A++EI+L+PL  A  T  LK+++ ++DC+ KLIA+ ++ G+    GG    + + ++  I
Sbjct: 77   AESEIILSPLINASSTGVLKIVDPAVDCIQKLIAHGYVRGEADPTGGPEALLLSKLIETI 136

Query: 169  CGCVDNSSSDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQATSK 228
            C C +       +L VLK LLTAV S   R+HG+ LL ++R CY I L S++ +NQAT+K
Sbjct: 137  CKCHELDDEGLELL-VLKTLLTAVTSISLRIHGDSLLQIVRTCYGIYLGSRNVVNQATAK 196

Query: 229  AMLTQMISIIFRRMETDQVSL-----------------STSSGTKESISAETSSKVVEET 288
            A L QM  I+FRRME D  ++                  +   T +S+    +  + +  
Sbjct: 197  ASLVQMSVIVFRRMEADSSTVPIQPIVVAELMEPMDKSESDPSTTQSVQGFITKIMQDID 256

Query: 289  AVTDENDKETTLGDALNSVKDTSLASVEELQNLAGGADIKGLEA---------------- 348
             V +  + + T G   +   +TSL       +L    D   L+A                
Sbjct: 257  GVFNSANAKGTFG-GHDGAFETSLPGTANPTDLLDSTDKDMLDAKYWEISMYKSALEGRK 316

Query: 349  --VLDKAVHTEDGKKMSRGIDLESVNIVKRDALLVFRTICKMGMK--EDADEVTTKTRIL 408
              + D  V  +D  ++  G      N ++RDA LVFR +CK+ MK     D    + +I+
Sbjct: 317  GELADGEVEKDDDSEVQIG------NKLRRDAFLVFRALCKLSMKTPPKEDPELMRGKIV 376

Query: 409  SLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALLRASVSQSPVIFQYATGIFSVLLLRF 468
            +LELL+ LLE     F     F+ ++K YL  +LL+ S S   +IFQ +  I   L+ RF
Sbjct: 377  ALELLKILLENAGAVFRTSDRFLGAIKQYLCLSLLKNSASNLMIIFQLSCSILLSLVSRF 436

Query: 469  RESLKGEIGIFFPLIVLRSLDGTDFP-VNQKISVLKMLEKICREPQILVDIFVNYDCDLE 528
            R  LK EIG+FFP+IVLR L+    P   QK+ VL+ L+K+C + QILVDIF+NYDCD+ 
Sbjct: 437  RAGLKAEIGVFFPMIVLRVLENVAQPDFQQKMIVLRFLDKLCVDSQILVDIFINYDCDVN 496

Query: 529  APNLFERMVTTLSKISQGTQNADPNMVAVSQTTSIKGSSLQCLVNVLKSLVDWEKSRLQ- 588
            + N+FERMV  L K +QG        +   Q  ++K  +++CLV VL+S+ DW   +L+ 
Sbjct: 497  SSNIFERMVNGLLKTAQGVPPGTVTTLLPPQEAAMKLEAMKCLVAVLRSMGDWVNKQLRL 556

Query: 589  ----SDKQSENV-RSLEEESSGYEN----------------LEIKSREDLTSNFEKAKAH 648
                S K  E V R+LEE S   EN                 E+ S        E+ +A+
Sbjct: 557  PDPYSAKMLEIVDRNLEEGSHPVENGKGDGGHGGFERSDSQSELSSGNSDALAIEQRRAY 616

Query: 649  KSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVAQFLRNTPSLDKTMIGDYLGQHEEF 708
            K  ++  IS FN+KP KG+E+LI    V ++P  +A FL++   L+KT+IGDYLG+ E+ 
Sbjct: 617  KLELQEGISIFNQKPKKGIEFLIKANKVGDSPEEIAAFLKDASGLNKTLIGDYLGEREDL 676

Query: 709  PVAVMHAYVDSMQFSGMKFDAAIRDFLKGFRLPGEAQKIDRIMEKFAERYCAGNPGLFKN 768
             + VMHAYVDS +F GM+FD AIR FL+GFRLPGEAQKIDRIMEKFAER+C  NP  F +
Sbjct: 677  SLKVMHAYVDSFEFQGMEFDEAIRAFLRGFRLPGEAQKIDRIMEKFAERFCKCNPKDFSS 736

Query: 769  ADTAYVLAYAVIMLNTDAHNPMVWPKMSRPDFIRMNVTNDPEECAPTELLEDIYDSIVKE 828
            ADTAYVLAY+VI+LNTDAHNPMV  KM+   FIR N   D  +  P E L  +Y+ I + 
Sbjct: 737  ADTAYVLAYSVILLNTDAHNPMVKSKMTADGFIRNNRGIDDGKDLPEEYLRALYERISRN 796

Query: 829  EIKMKDD-TSDRGKNRREGEERGGLVSILNLALPRRQSSTDAKSESEAIVKQTQVIFRNQ 888
            EIKMKDD    + K         GL +ILN+ +PRR    + ++ S+ +++  Q  F+ +
Sbjct: 797  EIKMKDDGLGPQQKQPTNSSRLLGLDTILNIVVPRRGDDMNMET-SDDLIRHMQERFKEK 856

Query: 889  GGK-RGIFYTSQRIELVRPMVEAVGWPLLATFSVTMEEGDNKPRVVLCMEGFRAGIHITH 948
              K   ++Y +  + ++R MVE    P+LA FSV +++ D+     LC+EGF   IH+T 
Sbjct: 857  ARKSESVYYAASDVIILRFMVEVCWAPMLAAFSVPLDQSDDAVITTLCLEGFHHAIHVTS 916

Query: 949  VLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTLLILCDLETESLQDTWNAVLECV 1008
            V+ + T R AF+TSL +FT LH+P +++ KN+EA++ ++ L + E   LQD W  +L CV
Sbjct: 917  VMSLKTHRDAFVTSLAKFTSLHSPADIKQKNIEAIKAIVKLAEEEGNYLQDAWEHILTCV 976

Query: 1009 SRLEFI----TSTPSIASTVMY-----GSNQISRDAVVQSLKE----------------- 1068
            SR E +       P  A+   +     G++ +++   V ++KE                 
Sbjct: 977  SRFEHLHLLGEGAPPDATFFAFPQTESGNSPLAKPNSVPAIKERAPGKLQYAASAMIRGS 1036

Query: 1069 -----LSGKPAD------------------------QVFVNSVKLPSDSVVEFFTALCGV 1128
                 ++GK ++                        ++F  S +L S+++++F  ALC V
Sbjct: 1037 YDGSGVAGKASNTVTSEQMNNLISNLNLLEQVGDMSRIFTRSQRLNSEAIIDFVKALCKV 1096

Query: 1129 SAEELKQ-TPARVFSLQKLVEISYYNMARIRMVWARIWSVLSNHFISAGSHHDEKIAMYA 1188
            S +EL+  +  RVFSL K+VEI++YNM RIR+VW+ IW VLS+ F++ G   +  IA++A
Sbjct: 1097 SMDELRSPSDPRVFSLTKIVEIAHYNMNRIRLVWSSIWHVLSDFFVTIGCSDNLSIAIFA 1156

Query: 1189 IDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSHSESIRSLIVDCIVQMIKSKVGN 1248
            +DSLRQL MK+LER ELAN+ FQN+ +KPFVV+MR S +  IR LI+ C+ QM+ S+V N
Sbjct: 1157 MDSLRQLSMKFLEREELANYNFQNEFMKPFVVVMRKSGAVEIRELIIRCVSQMVLSRVDN 1216

Query: 1249 IKSGWRSVFMIFTASADDESESIVESAFENVEQVILEHFDQVV---GDCFMDCVNCLIRF 1308
            +KSGW+S+FMIFT +A D  ++IV  +FE VE++I ++F  +       F DCVNCL+ F
Sbjct: 1217 VKSGWKSMFMIFTTAAHDAHKNIVFLSFEMVEKIIRDYFPHITETETTTFTDCVNCLVAF 1276

Query: 1309 ANNKSSHRISLKAIALLRICEDRLAEG-----------LIPGGALKPIHDSADPAFDVTE 1368
             N K    ISL+AIA L+ C  +LAEG           L P G      DS     +  E
Sbjct: 1277 TNCKFEKDISLQAIAFLQYCARKLAEGYVGSSLRRNPPLSPQGGKIGKQDSG-KFLESDE 1336

Query: 1369 HY--WFPMLAGLSDLTSDSRPEVRSCALEVLFDLLNERGAKFSTSFWESIFHRVLFPIFD 1397
            H   WFP+LAGLS+L+ D R E+R  AL+VLFD L   G  FS + WE +F  VLF IFD
Sbjct: 1337 HLYSWFPLLAGLSELSFDPRAEIRKVALKVLFDTLRNHGDHFSLALWERVFESVLFRIFD 1396

BLAST of MC05g0262 vs. TAIR 10
Match: AT3G60860.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 868.6 bits (2243), Expect = 8.6e-252
Identity = 628/1916 (32.78%), Postives = 959/1916 (50.05%), Query Frame = 0

Query: 25   PALQKAIQAYLDSTKEANQTQHSTPAETSQPATSAGDTSETGGEVDEARTVEETAEEAEN 84
            PAL+K +       K A+  +HS  A            +E    ++   +++++   + +
Sbjct: 16   PALEKIV-------KNASWRKHSKLA------------NECKAVIERLNSLQKSPPPSSS 75

Query: 85   VGRKAAPQEPISVVLANAGHV-LEGADAEIVLNPLRLAFDTKNLKVIELSLDCLHKLIAY 144
                +  +  +   L + G +    AD+E++ +PL  A  T   K+IE ++DC+ KLIA+
Sbjct: 76   AATDSESESSVPGPLNDGGSIEYSLADSELIFSPLINACGTGLAKIIEPAIDCIQKLIAH 135

Query: 145  DHLEGDPGLEGGKNVPIFTDILNMICGCVDNSSSDSTILQVLKVLLTAVASAKFRVHGEP 204
             ++ G+    GG    +   +++ +C C D    +S  L VLK LL+A+ S   R+HG+ 
Sbjct: 136  GYIRGESDPSGGAESLLLFKLIDSVCKCHD-LGDESIELPVLKTLLSAINSISLRIHGKC 195

Query: 205  LLGVIRVCYNIALHSKSPINQATSKAMLTQMISIIFRRMETDQVSL-------------- 264
            LL V+R CY+I L SK+ +NQ T+KA L Q++ I+FRRME D  ++              
Sbjct: 196  LLLVVRTCYDIYLGSKNVVNQTTAKASLIQILVIVFRRMEADSSTVPIQPIVVAELMEPL 255

Query: 265  --STSSGTKESISAETSSKVVEET------------AVTDENDKETTLG-DALNSVKDTS 324
              S + GT         +K++++             + +    ++   G   + +   T 
Sbjct: 256  EKSDADGTMTQFVQGFITKIMQDIDGVLNPTMSGSGSGSGSGGQDGAYGTTTVETTNPTD 315

Query: 325  LASVEELQNL-AGGADIKGLEAVLD--KAVHTEDGKKMSRGIDLESVNIVKRDALLVFRT 384
            L    +   L A   +I   ++ L+  K   T+   +    ++++  N ++RDA LVFR 
Sbjct: 316  LLDSTDKDMLDAKYWEISMYKSALEGRKGELTDGDAERDDDLEVQIENKLRRDACLVFRA 375

Query: 385  ICKMGMK-----EDADEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAYLSYALL 444
            +CK+ MK       AD  + + +IL+LELL+ LLE     F     F   +K +L  +LL
Sbjct: 376  LCKLSMKAPPKESSADPQSMRGKILALELLKILLENAGAVFRTSEKFSADIKQFLCLSLL 435

Query: 445  RASVSQSPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFP-VNQKISVL 504
            + S S   +IFQ +  IF  L+ RFR  LK EIG+FFP+IVLR ++    P   QK+ VL
Sbjct: 436  KNSASTLMIIFQLSCSIFISLVARFRAGLKAEIGVFFPMIVLRVVENVAQPNFQQKMIVL 495

Query: 505  KMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAVSQTTSI 564
            + L+K+C + QILVDIF+NYDCD+ + N+FERMV  L K +QG        +   Q  ++
Sbjct: 496  RFLDKLCLDSQILVDIFLNYDCDVNSSNIFERMVNGLLKTAQGVPPGTATTLMPPQEAAM 555

Query: 565  KGSSLQCLVNVLKSLVDWEKSRLQ-----SDKQSENVR-------------SLEEESSGY 624
            K  +++CLV +LKS+ DW   +L+     S  +S+ +              + +E + G 
Sbjct: 556  KLEAMKCLVAILKSMGDWLNKQLRLPVSNSLNKSDVIEIDLGPGSPQLANGNADESADGS 615

Query: 625  ENL-EIKSREDLTSNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSVAQF 684
            +   E           E+ +A+K  ++  IS FNRKP KG+E+LI+   V  +P  +A F
Sbjct: 616  DTYSESSGGTSDALAIEQRRAYKLELQEGISLFNRKPTKGIEFLINAGKVGESPEEIAGF 675

Query: 685  LRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMQFSGMKFDAAIRDFLKGFRLPGEAQK 744
            L++   L+KT+IGDYLG+ E+  + VMHAYVDS  F GM+FD AIR FL+GFRLPGEAQK
Sbjct: 676  LKDASGLNKTLIGDYLGEREDLALKVMHAYVDSFDFRGMEFDEAIRTFLEGFRLPGEAQK 735

Query: 745  IDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSRPDFIRMNVT 804
            IDRIMEKFAERYC  NP +F +AD+AYVLAY+VIMLNTDAHNPMV  KMS  DFIR N  
Sbjct: 736  IDRIMEKFAERYCKCNPKVFTSADSAYVLAYSVIMLNTDAHNPMVKNKMSADDFIRNNRG 795

Query: 805  NDPEECAPTELLEDIYDSIVKEEIKMK-DDTSDRGKNRREGEERGGLVSILNLALPRRQS 864
             D  +  P + +  +Y+ I K EIKMK DD   + K         GL  ILN+ + R+Q 
Sbjct: 796  IDDGKDLPADYMRSLYERITKHEIKMKEDDLRLQQKQYANSNRMLGLDGILNIVI-RKQW 855

Query: 865  STDAKSESEAIVKQTQVIFRNQGGK-RGIFYTSQRIELVRPMVEAVGWPLLATFSVTMEE 924
                   S+ ++K  Q  F+ +  K    +Y +  + ++R M+EA   P+LA FSV +++
Sbjct: 856  GDSYAETSDDLMKHMQEQFKEKARKSESTYYAATDVVILRFMIEACWAPMLAAFSVPLDQ 915

Query: 925  GDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRTL 984
             D+   + +C+EGF   IH T ++ M T R AF+TSL +FT LH+P +++ +N+EA++ +
Sbjct: 916  SDDLIVINICLEGFHHAIHATSLMSMKTHRDAFVTSLAKFTSLHSPADIKQRNIEAIKAI 975

Query: 985  LILCDLETESLQDTWNAVLECVSRLE--------------FITSTPS------------- 1044
            L L D E   LQD W  +L CVSR E              F  S  +             
Sbjct: 976  LRLADEEGNYLQDAWEHILTCVSRFEQLHLLGEGAPPDATFFASKQNESEKSKQPKQYIL 1035

Query: 1045 -------------IASTVMYGS--------------NQISRDAVVQSLKELSG-KPADQV 1104
                          A+ V+ GS               Q    ++V +L  L      +QV
Sbjct: 1036 PVLKRKGPGKSQYAATGVLRGSYDSMSLGGKGSKNVRQEQMSSIVSNLNLLEQVGEMNQV 1095

Query: 1105 FVNSVKLPSDSVVEFFTALCGVSAEELKQ-TPARVFSLQKLVEISYYNMARIRMVWARIW 1164
            F  S KL S+++++F  ALC VS +EL+  +  RVFSL K+VEI++YNM RIR+VW+ IW
Sbjct: 1096 FSQSQKLNSEAIIDFVKALCKVSMDELRSPSNPRVFSLTKIVEIAHYNMNRIRLVWSSIW 1155

Query: 1165 SVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSH 1224
             VLS  F++ G   +  IA++A+DSLRQL MK+LER ELAN+ FQN+ + PFV++MR S+
Sbjct: 1156 QVLSGFFVTIGCSENLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMTPFVIVMRRSN 1215

Query: 1225 SESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEH 1284
               IR LI+ C+ QM+ S+V N+KSGW+S+FM+FT +A D+ ++IV  +FE +E++I E+
Sbjct: 1216 DVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVFLSFEIIEKIIREY 1275

Query: 1285 FDQVV---GDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAE------------ 1344
            F  +       F DCVNCL+ F NN+ S  ISL +IA LR C  +LAE            
Sbjct: 1276 FPYITETETTTFTDCVNCLVAFTNNRFSKDISLSSIAFLRYCATKLAEGDLNSPSTNKYK 1335

Query: 1345 ---GLIPGGALKPIHDSADPAFDVTE----HYWFPMLAGLSDLTSDSRPEVRSCALEVLF 1404
               G IP  +L           ++      ++WFP+L+GLS+L+ D RPE+R  AL+++F
Sbjct: 1336 GTSGKIPQSSLHSGKSGKQENGEIVNNNHLYFWFPLLSGLSELSFDPRPEIRKSALQIMF 1395

Query: 1405 DLLNERGAKFSTSFWESIFHRVLFPIFDHLRQ----PGKENLITSG-----------DEW 1464
            D L   G  FS   WE +F  VLFPIFD++R      G++     G           D W
Sbjct: 1396 DTLRNHGHLFSLPLWEKVFESVLFPIFDYVRHSIDPSGEDESADQGSSGGEVDELDHDAW 1455

Query: 1465 LRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKQPEQSVVSLALGALVHLIEVG 1524
            L ET   +LQL+ +LF  FY  V  +L  +L LL+   K+P QS+  + + A V L+   
Sbjct: 1456 LYETCTLALQLVVDLFVKFYTTVNPLLEKVLMLLVSFIKRPHQSLAGIGIAAFVRLMSDA 1515

Query: 1525 GHQFSEDDWDTLLKSIRDASYTTQPLELLNALGFENPSHAEVNIVDGISLKSVSQQEAKN 1584
               FSE+ W  ++ ++++A+ TT P               + +        + SQ+ A N
Sbjct: 1516 DGLFSEEKWLEVVSALKEAAKTTCP---------------DFSYFLSEEYVARSQRSALN 1575

Query: 1585 HQFDVGDHGKASPVASPRGESITRNPIEESGLQITSDEFAEGLPSPSTRATKAAEGGSLQ 1644
             Q    +   A+P A+   E                                       Q
Sbjct: 1576 IQNSNAE--SAAPTATDGNE-------------------------------------ESQ 1635

Query: 1645 RSQTIGQRIMGNMMDNIFVRSLTSKSKGRASDASVPSSPIKLPPDTLDPEVKEDEESSLM 1704
            R+ T              + +  S +K RA+                             
Sbjct: 1636 RTAT-------------HLYAAISDAKCRAA----------------------------- 1695

Query: 1705 AIVRGKCITQLLLLGVIDGIQKKYWFKLNAPQKIAIMDILLSLLEFAATYNSYNNLRQRM 1764
                     QLLL+  +  I   Y  +L+A   + ++D L  +   A   NS   LR R+
Sbjct: 1696 --------VQLLLIQAVMEIYNMYRPQLSAKNTLVLVDALHGVALHAHGINSNTILRSRL 1755

Query: 1765 HHIPD----ERPPLNLLRQELAGTSVYLDILLK-ATSRFNTLEAEEQKFVDNLEVETASP 1781
              +      + PP  LLR E     + L  L      +    E EE++ +++L V     
Sbjct: 1756 QELGPMTQMQDPP--LLRLENESYQICLTFLQNLVADKTKKEEEEEEEEIESLLVNICQE 1789

BLAST of MC05g0262 vs. TAIR 10
Match: AT4G38200.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 831.2 bits (2146), Expect = 1.5e-240
Identity = 522/1395 (37.42%), Postives = 775/1395 (55.56%), Query Frame = 0

Query: 106  LEGADAEIVLNPLRLAFDTKNLKVIELSLDCLHKLIAYDHLEGDPGLEGGKNVPIFTDIL 165
            L  +DA+ VL PL L+ DT   KVIE +LDC  KL +   L G+  +       +   ++
Sbjct: 63   LTTSDADAVLQPLLLSLDTGYAKVIEPALDCSFKLFSLSLLRGE--VCSSSPDSLLYKLI 122

Query: 166  NMICGCVDNSSSDSTILQVLKVLLTAVASAKFRVHGEPLLGVIRVCYNIALHSKSPINQA 225
            + IC  V     +S  L VL+VLL AV S +  + G+ LL ++R CYN+ L   +  NQ 
Sbjct: 123  HAICK-VCGIGEESIELAVLRVLLAAVRSPRILIRGDCLLHLVRTCYNVYLGGFNGTNQI 182

Query: 226  TSKAMLTQMISIIFRRMETDQVSLSTSSGTKESISAETSSKVVEETAVTDENDKETTLGD 285
             +K++L Q++ I+F R E + +  S             +  V +  A+TD+N  E   G+
Sbjct: 183  CAKSVLAQIMLIVFTRSEANSMDASLK-----------TVNVNDLLAITDKNVNE---GN 242

Query: 286  ALNSVKDTSLASVEELQNLAGGADIKGLEAVLDKAVHTEDGKKMSRGIDLESVNIVKRDA 345
            +++ +    +  V      A   D   ++   + A  TED    S+         ++ D 
Sbjct: 243  SVH-ICQGFINDVITAGEAAPPPDFALVQPPEEGASSTEDEGTGSK---------IREDG 302

Query: 346  LLVFRTICKMGMKEDA-----DEVTTKTRILSLELLQGLLEGVSQTFTKDFHFIDSVKAY 405
             L+F+ +CK+ MK  +     D++  + + LSLELL+ +++     +  D  F++++K  
Sbjct: 303  FLLFKNLCKLSMKFSSQENTDDQILVRGKTLSLELLKVIIDNGGPIWLSDERFLNAIKQL 362

Query: 406  LSYALLRASVSQSPVIFQYATGIFSVLLLRFRESLKGEIGIFFPLIVLRSLDGTDFP-VN 465
            L  +LL+ S      IFQ    IF+ LL ++R  +K E+GIFFP++VLR L+    P   
Sbjct: 363  LCLSLLKNSALSVMSIFQLQCAIFTTLLRKYRSGMKSEVGIFFPMLVLRVLENVLQPSFV 422

Query: 466  QKISVLKMLEKICREPQILVDIFVNYDCDLEAPNLFERMVTTLSKISQGTQNADPNMVAV 525
            QK++VL +LE IC +P +++DIFVN+DCD+E+PN+FER+V  L K + G       +++ 
Sbjct: 423  QKMTVLSLLENICHDPNLIIDIFVNFDCDVESPNIFERIVNGLLKTALGPPPGSSTILSP 482

Query: 526  SQTTSIKGSSLQCLVNVLKSLVDWEKSRLQSDKQSENVRSLEEESSGYENLEIKSREDLT 585
             Q  + +  S++CLV+++K++  W   +L S   S   +SLE E+    +      +  T
Sbjct: 483  VQDITFRHESVKCLVSIIKAMGTWMDQQL-SVGDSLLPKSLENEAPANNHSNSNEEDGTT 542

Query: 586  ----------------SNFEKAKAHKSTVEAAISEFNRKPVKGVEYLISNKLVENTPSSV 645
                            +  E+ +A+K   +  ++ FNRKP KG+E+LIS+K V N+P  V
Sbjct: 543  IDHDFHPDLNPESSDAATLEQRRAYKIERQKGVTLFNRKPSKGIEFLISSKKVGNSPDEV 602

Query: 646  AQFLRNTPSLDKTMIGDYLGQHEEFPVAVMHAYVDSMQFSGMKFDAAIRDFLKGFRLPGE 705
              FLRNT  L+ TMIGDYLG+ E+FP+ VMHAYVDS  F  M F  AIR FL+GFRLPGE
Sbjct: 603  VSFLRNTTGLNATMIGDYLGEREDFPMKVMHAYVDSFDFKEMNFGEAIRFFLRGFRLPGE 662

Query: 706  AQKIDRIMEKFAERYCAGNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSRPDFIRM 765
            AQKIDRIMEKFAER+C  NP  F +ADTAYVLAY+VIMLNTDAHN MV  KM++ DFIR 
Sbjct: 663  AQKIDRIMEKFAERFCKCNPNSFSSADTAYVLAYSVIMLNTDAHNIMVKEKMTKADFIRN 722

Query: 766  NVTNDPEECAPTELLEDIYDSIVKEEIKMKDDTSDRGKNRREG-EERGGLVSILNLALPR 825
            N   D  +  P E L  +YD +V  EIKM  D+S     +  G  +  GL  ILNL    
Sbjct: 723  NRGIDDGKDLPEEYLGALYDQVVINEIKMSSDSSAPESRQSNGLNKLLGLDGILNLVY-W 782

Query: 826  RQSSTDAKSESEAIVKQTQVIFRNQGGK-RGIFYTSQRIELVRPMVEAVGWPLLATFSVT 885
             Q+   A   +  ++K  Q  FR++ GK    ++    + ++R MVE    P+LA FSVT
Sbjct: 783  TQTEEKAVGANGLLIKDIQEKFRSKSGKSESAYHVVTDVAILRFMVEVSWGPMLAAFSVT 842

Query: 886  MEEGDNKPRVVLCMEGFRAGIHITHVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEAL 945
            +++ D++   V C+ GFR  +H+T V+GM T R AF+TS+ +FT LH   +M+ KNV+A+
Sbjct: 843  LDQSDDRLAAVECLRGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNLHCAGDMKQKNVDAV 902

Query: 946  RTLLILCDLETESLQDTWNAVLECVSRLEFI----TSTPSIAS----------------- 1005
            + ++ +   +   LQD W  +L C+SR+E +       PS AS                 
Sbjct: 903  KAIISIAIEDGNHLQDAWEHILTCLSRIEHLQLLGEGAPSDASYFASTETEEKKALGFPN 962

Query: 1006 ---------TVM--------YGSNQISRD--------------AVVQSLKELSGKPADQV 1065
                      VM        Y S+ I  +              A +  L ++     + V
Sbjct: 963  LKKKGALQNPVMMAVVRGGSYDSSTIGPNMPGLVKQDQINNFIANLNLLDQIGSFQLNNV 1022

Query: 1066 FVNSVKLPSDSVVEFFTALCGVSAEELKQ-TPARVFSLQKLVEISYYNMARIRMVWARIW 1125
            + +S +L ++++V F  ALC VS  EL+  T  RVFSL KLVEI++YNM RIR+VW+RIW
Sbjct: 1023 YAHSQRLKTEAIVAFVKALCKVSMSELQSPTDPRVFSLTKLVEIAHYNMNRIRLVWSRIW 1082

Query: 1126 SVLSNHFISAGSHHDEKIAMYAIDSLRQLGMKYLERAELANFTFQNDILKPFVVLMRNSH 1185
            S+LS+ F+S G   +  +A++ +DSLRQL MK+LER ELAN+ FQN+ L+PFV++M+ S 
Sbjct: 1083 SILSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANYNFQNEFLRPFVIVMQKSS 1142

Query: 1186 SESIRSLIVDCIVQMIKSKVGNIKSGWRSVFMIFTASADDESESIVESAFENVEQVILEH 1245
            S  IR LIV CI QM+ S+V N+KSGW+SVF +FT +A DE ++IV  AFE +E+++ E+
Sbjct: 1143 SAEIRELIVRCISQMVLSRVSNVKSGWKSVFKVFTTAAADERKNIVLLAFETMEKIVREY 1202

Query: 1246 FDQVV---GDCFMDCVNCLIRFANNKSSHRISLKAIALLRICEDRLAEGLI-------PG 1305
            F  +       F DCV CLI F N+  +  +SL AIA LR C  +LA+G +         
Sbjct: 1203 FSYITETEATTFTDCVRCLITFTNSTFTSDVSLNAIAFLRFCALKLADGGLVWNEKGRSS 1262

Query: 1306 GALKPIHDSADPA----FDVTEH--YWFPMLAGLSDLTSDSRPEVRSCALEVLFDLLNER 1365
                P+ D   P+     D  E+  YW P+L GLS LTSDSR  +R  +LEVLF++L + 
Sbjct: 1263 SPSTPVTDDHSPSTQNFMDADENISYWVPLLTGLSKLTSDSRSAIRKSSLEVLFNILKDH 1322

Query: 1366 GAKFSTSFWESIFHRVLFPIF-------------DHLRQPGKENLITSGDEWLRETSIHS 1395
            G  FS +FW  +F  V++PIF             +H   P   +   S   W  ETS  +
Sbjct: 1323 GHIFSRTFWIGVFSSVIYPIFNSVWGENDLLSKDEHSSFPSTFSSHPSEVSWDAETSAMA 1382