MC05g0248 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0248 vs. ExPASy Swiss-Prot
Match: Q9LQL2 (Protein NRT1/ PTR FAMILY 7.3 OS=Arabidopsis thaliana OX=3702 GN=NPF7.3 PE=1 SV=2)

HSP 1 Score: 841.6 bits (2173), Expect = 5.3e-243
Identity = 421/599 (70.28%), Postives = 489/599 (81.64%), Query Frame = 0

Query: 1   MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ 60
           M+CL        +  K T K+   E+E  T DG VD +G P+IR  +G W AGI+IL+NQ
Sbjct: 1   MSCLE-------IYNKDTMKKKEGEEE--TRDGTVDYYGRPSIRSNSGQWVAGIVILLNQ 60

Query: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120
           GLATLAFFGVGVNLVLFLTRV+ Q+NA AANNVSKWTGTVYIFSL+GAFLSDSYWGR+KT
Sbjct: 61  GLATLAFFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKT 120

Query: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGG 180
           CAIFQ IFVIGL+SLS++SY+FL+RP+GCGDE TPC SHS   + +FY S+YL+ALG GG
Sbjct: 121 CAIFQVIFVIGLSSLSLSSYMFLIRPRGCGDEVTPCGSHSMMEITMFYFSIYLIALGYGG 180

Query: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240
           YQPNIAT GADQFDEE PKEG SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMW LGFW
Sbjct: 181 YQPNIATLGADQFDEEHPKEGYSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWALGFW 240

Query: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQI-MTDSEGLFDED 300
           AST SA + L+LFL G PRYR+F P GNPLSR  QV++AAT+K  V+  +   E ++D D
Sbjct: 241 ASTGSAIIGLILFLVGTPRYRYFKPTGNPLSRFCQVLVAATKKSSVEAPLRGREEMYDGD 300

Query: 301 D--KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCIL 360
              K    N  R+I+HT+ FKFLD+AA IT+ + D  +  + NPWRLC VTQVEEVKCIL
Sbjct: 301 SEGKNASVNTGRRIVHTDEFKFLDKAAYITARDLDDKKQDSVNPWRLCPVTQVEEVKCIL 360

Query: 361 RLLPIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYR 420
           RL+PIWLCTI+YSVVFTQMASLFVEQGAAM T++S F IPPASMSSFDILSVA FIF+YR
Sbjct: 361 RLMPIWLCTIIYSVVFTQMASLFVEQGAAMNTSVSDFKIPPASMSSFDILSVALFIFLYR 420

Query: 421 RVIDPVYARLTKS---SLTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSS 480
           RV++PV  R  K+    +TEL RMGIGLVIA+ AM++AG VE +RLK+A + C HCDGSS
Sbjct: 421 RVLEPVANRFKKNGSKGITELHRMGIGLVIAVIAMIAAGIVECYRLKYADKSCTHCDGSS 480

Query: 481 SLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLV 540
           SLSIFWQ PQY LIGASEVFMYVGQLEFFN+Q PDGLKSFGSALCM S+S GN+VSSLLV
Sbjct: 481 SLSIFWQAPQYSLIGASEVFMYVGQLEFFNAQTPDGLKSFGSALCMMSMSMGNFVSSLLV 540

Query: 541 TIVMKISATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESRE 594
           T+V+KIS  D+MPGWIP NLN+GHLDRF+FLLAALT+ DL VYI CAKWYK I+ E ++
Sbjct: 541 TMVVKISTEDHMPGWIPRNLNKGHLDRFYFLLAALTSIDLVVYIACAKWYKPIQLEGKD 590

BLAST of MC05g0248 vs. ExPASy Swiss-Prot
Match: Q8GXN2 (Protein NRT1/ PTR FAMILY 7.2 OS=Arabidopsis thaliana OX=3702 GN=NPF7.2 PE=2 SV=2)

HSP 1 Score: 815.1 bits (2104), Expect = 5.3e-235
Identity = 412/576 (71.53%), Postives = 470/576 (81.60%), Query Frame = 0

Query: 23  ISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVV 82
           + + E CT DG+VDRHG+PAIR  TG W   ILILVNQGLATLAFFGVGVNLVLFLTRV+
Sbjct: 5   VRQFEVCTQDGSVDRHGNPAIRANTGKWLTAILILVNQGLATLAFFGVGVNLVLFLTRVM 64

Query: 83  GQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIF 142
           GQDNA AANNVSKWTGTVYIFSLLGAFLSDSYWGR+KTCAIFQA FV GL  LS+++   
Sbjct: 65  GQDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRYKTCAIFQASFVAGLMMLSLSTGAL 124

Query: 143 LVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGL 202
           L+ P GCG E +PC  HS+F   LFYLSVYL+ALG GGYQPNIATFGADQFD ED  EG 
Sbjct: 125 LLEPSGCGVEDSPCKPHSTFKTVLFYLSVYLIALGYGGYQPNIATFGADQFDAEDSVEGH 184

Query: 203 SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRH 262
           SKIAFFSYFYLALNLGSLFSNT+LGYFED+G W LGFWAS  SA   L+LFL G P+YRH
Sbjct: 185 SKIAFFSYFYLALNLGSLFSNTVLGYFEDQGEWPLGFWASAGSAFAGLVLFLIGTPKYRH 244

Query: 263 FTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDR 322
           FTP+ +P SR  QV++AATRK K+ +  +   L+   D E    G +KILHT GF+FLDR
Sbjct: 245 FTPRESPWSRFCQVLVAATRKAKIDVHHEELNLY---DSETQYTGDKKILHTKGFRFLDR 304

Query: 323 AAVIT-SSESDQLEDGAR-NPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLF 382
           AA++T   E++++E G++ +PWRLC+VTQVEEVKC+LRLLPIWLCTILYSVVFTQMASLF
Sbjct: 305 AAIVTPDDEAEKVESGSKYDPWRLCSVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLF 364

Query: 383 VEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPVYARLTKS----SLTELQ 442
           V QGAAM+TNI  F IP +SMSSFDILSVA FIF YRR +DP++ARL K+     LTELQ
Sbjct: 365 VVQGAAMKTNIKNFRIPASSMSSFDILSVAFFIFAYRRFLDPLFARLNKTERNKGLTELQ 424

Query: 443 RMGIGLVIAICAMVSAGTVEIFRLKH-ATRDCLHCDGSSSLSIFWQIPQYVLIGASEVFM 502
           RMGIGLVIAI AM+SAG VEI RLK+           SS+LSIFWQ+PQY+LIGASEVFM
Sbjct: 425 RMGIGLVIAIMAMISAGIVEIHRLKNKEPESATSISSSSTLSIFWQVPQYMLIGASEVFM 484

Query: 503 YVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLN 562
           YVGQLEFFNSQAP GLKSF SALCM SIS GNYVSSLLV+IVMKIS TD++ GWIP NLN
Sbjct: 485 YVGQLEFFNSQAPTGLKSFASALCMASISLGNYVSSLLVSIVMKISTTDDVHGWIPENLN 544

Query: 563 RGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFES 592
           +GHL+RF+FLLA LTAAD  VY++CAKWYKYIK E+
Sbjct: 545 KGHLERFYFLLAGLTAADFVVYLICAKWYKYIKSEA 577

BLAST of MC05g0248 vs. ExPASy Swiss-Prot
Match: Q3E9B5 (Protein NRT1/ PTR FAMILY 7.1 OS=Arabidopsis thaliana OX=3702 GN=NPF7.1 PE=2 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 3.3e-184
Identity = 337/547 (61.61%), Postives = 398/547 (72.76%), Query Frame = 0

Query: 46  KTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSL 105
           K G W   I++LVNQGLATLAFFGVGVNLVLFLTRV+GQ NA AANNVSKWTGTVY+FSL
Sbjct: 59  KNGGWTNAIILLVNQGLATLAFFGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFSL 118

Query: 106 LGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMA 165
           +GAFLSDSYWGR+ TC IFQ IFVIG+  LS  S+ FL++P+GCGD    CN  SS  +A
Sbjct: 119 VGAFLSDSYWGRYLTCTIFQVIFVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGVA 178

Query: 166 LFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTI 225
           +FYLSVYLVA G GG+QP +ATFGADQ D++      SK AFFSYFY ALN+G+LFSNTI
Sbjct: 179 IFYLSVYLVAFGYGGHQPTLATFGADQLDDDKN----SKAAFFSYFYFALNVGALFSNTI 238

Query: 226 LGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWK 285
           L YFED+G+W  GF  S  SA +AL+ FL    +YR+  P GNPL RV+QV +A  RKW 
Sbjct: 239 LVYFEDKGLWTEGFLVSLGSAIVALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKWS 298

Query: 286 VQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGAR-NPWRL 345
           V    D   L++ +  E    G+RKI H+  F FLDRAAVIT ++     +G R N WRL
Sbjct: 299 VVRPGDPHELYELEGPESAIKGSRKIFHSTKFLFLDRAAVITEND----RNGTRSNAWRL 358

Query: 346 CTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSF 405
           C+VTQVEE KC+++LLPIWLCTI+YSV+FTQMASLFVEQG  M   +  FHIP ASMS F
Sbjct: 359 CSVTQVEEAKCVMKLLPIWLCTIIYSVIFTQMASLFVEQGDVMNAYVGKFHIPAASMSVF 418

Query: 406 DILSVAAFIFIYRRVIDPVYARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHAT 465
           DI SV     IYR +I P Y R      TEL RMGIGL+I I AMV+AG  EI RLK   
Sbjct: 419 DIFSVFVSTGIYRHIIFP-YVR-----PTELMRMGIGLIIGIMAMVAAGLTEIQRLKRVV 478

Query: 466 RDCLHCDGSSSLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSIS 525
                    S L+I WQIPQYVL+GASEVFMYVGQLEFFN QAPDGLK+ GS+LCM S++
Sbjct: 479 PG----QKESELTILWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMASMA 538

Query: 526 FGNYVSSLLVTIVMKISAT-DNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKW 585
            GNYVSSL+V IVM I+   +N PGWIP NLN GH+DRF+FL+AAL A D  VY++ AKW
Sbjct: 539 LGNYVSSLMVNIVMAITKRGENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIFAKW 587

Query: 586 YKYIKFE 591
           Y+ I  +
Sbjct: 599 YQPISHD 587

BLAST of MC05g0248 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 8.4e-140
Identity = 257/573 (44.85%), Postives = 383/573 (66.84%), Query Frame = 0

Query: 23  ISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVV 82
           + EK+  T DG VD H +PA ++KTG W A   IL N+    LA++G+G NLV +L   +
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRL 60

Query: 83  GQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIF 142
            Q NA AANNV+ W+GT YI  L+GAF++D+Y GR+ T A F  I+V G+  L++++ + 
Sbjct: 61  NQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVP 120

Query: 143 LVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGL 202
            ++P  C  +   C+ +SS   A+F++++Y++ALG GG +P +++FGADQFDE D  E +
Sbjct: 121 GLKPGNCNAD--TCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKI 180

Query: 203 SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRH 262
            K +FF++FY ++N+G+L + T+L + +    W  GF   T +  +A+  F  G   YR 
Sbjct: 181 KKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRL 240

Query: 263 FTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDR 322
             P G+PL+R+ QV++AA RK  V++  D   LF+  D E    G+RK++HT+  KF D+
Sbjct: 241 QRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDK 300

Query: 323 AAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVE 382
           AAV   S+SD ++DG  NPWRLC+VTQVEE+K I+ LLP+W   I+++ V++QM+++FV 
Sbjct: 301 AAV--ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVL 360

Query: 383 QGAAMRTNI-STFHIPPASMSSFDILSVAAFIFIYRRVIDPVYARLTKS--SLTELQRMG 442
           QG  M  ++   F IP AS+S FD +SV  +  +Y + I P+  + T++    T+LQRMG
Sbjct: 361 QGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMG 420

Query: 443 IGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFWQIPQYVLIGASEVFMYVGQ 502
           IGLV++I AM++AG +E+ RL +      +      +SIFWQIPQY+LIG +EVF ++GQ
Sbjct: 421 IGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQ 480

Query: 503 LEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLNRGHL 562
           LEFF  QAPD ++S  SAL +T+++ GNY+S++LVT+VMKI+  +  PGWIP NLNRGHL
Sbjct: 481 LEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHL 540

Query: 563 DRFFFLLAALTAADLAVYILCAKWYKYIKFESR 593
           D FF+LLA L+  +  VY+  +K YKY K   R
Sbjct: 541 DYFFYLLATLSFLNFLVYLWISKRYKYKKAVGR 568

BLAST of MC05g0248 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.9e-131
Identity = 240/565 (42.48%), Postives = 365/565 (64.60%), Query Frame = 0

Query: 32  DGAVDRHGHPAIRDKTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVVGQDNAAAAN 91
           DG+VD +G+P +++KTG W A   IL N+    LA++G+  NL+ +LT  + Q N +AA 
Sbjct: 27  DGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAAT 86

Query: 92  NVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIFLVRPKGC-G 151
           NV+ W GT Y+  L+GA L+D+YWGR+ T A F  I+ IG+++L++++ +  ++P  C G
Sbjct: 87  NVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECIG 146

Query: 152 DEQTPCNSHSSFHMALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSKIAFFSY 211
           D    C S +    A+F+  +YL+ALG GG +P +++FGADQFD+ D +E + K +FF++
Sbjct: 147 DF---CPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNW 206

Query: 212 FYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRHFTPKGNPL 271
           FY ++N+G+L S+++L + ++   W LGF   T    +A+  F  G P YR   P G+P+
Sbjct: 207 FYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPI 266

Query: 272 SRVSQVVIAATRKWKVQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDRAAVITSSE 331
           +R+SQVV+A+ RK  V++  D+  L++  DK     G+RKI HT+  ++LD+AAVI  SE
Sbjct: 267 TRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVI--SE 326

Query: 332 SDQLEDGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVEQGAAMRTN 391
            +       N WRLCTVTQVEE+K ++R+ PIW   I++S V+ QM+++FV+QG AM   
Sbjct: 327 EESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCK 386

Query: 392 ISTFHIPPASMSSFDILSVAAFIFIYRRVIDPVYARLT--KSSLTELQRMGIGLVIAICA 451
           I +F +PPA++ +FD  SV  ++ +Y R I P+  + T      TE+QRMGIGL +++  
Sbjct: 387 IGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLC 446

Query: 452 MVSAGTVEIFRLKHATRD--CLHCDGSSSLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQ 511
           M +A  VEI RL H   D   +       +S+ WQIPQY ++GA+EVF ++GQLEFF  Q
Sbjct: 447 MAAAAIVEIIRL-HMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQ 506

Query: 512 APDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLNRGHLDRFFFLL 571
           +PD ++S  SAL + + + GNY+SSL++T+V   +  +   GWI  NLN GHLD FF+LL
Sbjct: 507 SPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFWLL 566

Query: 572 AALTAADLAVYILCAKWYKYIKFES 592
           A L+  ++AVY   A  YK  K  S
Sbjct: 567 AGLSLVNMAVYFFSAARYKQKKASS 585

BLAST of MC05g0248 vs. NCBI nr
Match: XP_022146964.1 (protein NRT1/ PTR FAMILY 7.3-like [Momordica charantia])

HSP 1 Score: 1187 bits (3071), Expect = 0.0
Identity = 595/598 (99.50%), Postives = 596/598 (99.67%), Query Frame = 0

Query: 1   MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ 60
           MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ
Sbjct: 1   MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ 60

Query: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120
           GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT
Sbjct: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120

Query: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGG 180
           CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNS SSFHMALFYLSVYLVALGNGG
Sbjct: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSRSSFHMALFYLSVYLVALGNGG 180

Query: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240
           YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW
Sbjct: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240

Query: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDD 300
           ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAAT KWKVQIMTDSEGLFDEDD
Sbjct: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATTKWKVQIMTDSEGLFDEDD 300

Query: 301 KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLL 360
           KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLL
Sbjct: 301 KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLL 360

Query: 361 PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI 420
           PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI
Sbjct: 361 PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI 420

Query: 421 DPVYARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFW 480
           DPVYARLTKSS+TELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFW
Sbjct: 421 DPVYARLTKSSVTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFW 480

Query: 481 QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI 540
           QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI
Sbjct: 481 QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI 540

Query: 541 SATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAAV 598
           SATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAAV
Sbjct: 541 SATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAAV 598

BLAST of MC05g0248 vs. NCBI nr
Match: XP_038878093.1 (protein NRT1/ PTR FAMILY 7.3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1120 bits (2898), Expect = 0.0
Identity = 558/597 (93.47%), Postives = 578/597 (96.82%), Query Frame = 0

Query: 1   MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ 60
           M  LN SCFRQ+  +K  KK  I EKE CTLDGAVDRHG PAIRDKTGTW AGILILVNQ
Sbjct: 1   MTYLNISCFRQSTTKK--KKMAIPEKEACTLDGAVDRHGRPAIRDKTGTWVAGILILVNQ 60

Query: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120
           GLATLAFFGVGVNLVLFLTRVVGQDNA AANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT
Sbjct: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120

Query: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGG 180
           CAIFQAIFVIGLASLSITSYIFLVRPKGCGDE TPCNSHSSFH+ALFYLSVYLVALGNGG
Sbjct: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEHTPCNSHSSFHIALFYLSVYLVALGNGG 180

Query: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240
           YQPNIATFGADQFDEEDPKEG+SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW
Sbjct: 181 YQPNIATFGADQFDEEDPKEGISKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240

Query: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDD 300
           ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVV+AATRKWKVQ+M +SEGLFD+D+
Sbjct: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVVAATRKWKVQMMPNSEGLFDDDE 300

Query: 301 KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLL 360
           KELV+NGAR+ILHTNGFKFLDRAAVITSSE+D+L+DGARNPWRLCTVTQVEEVKCILRLL
Sbjct: 301 KELVNNGARRILHTNGFKFLDRAAVITSSETDRLDDGARNPWRLCTVTQVEEVKCILRLL 360

Query: 361 PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI 420
           PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI
Sbjct: 361 PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI 420

Query: 421 DPVYARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFW 480
           DPV+ARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHA ++CLHCD SSSLSIFW
Sbjct: 421 DPVFARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHANKECLHCDDSSSLSIFW 480

Query: 481 QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI 540
           QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMK+
Sbjct: 481 QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKV 540

Query: 541 SATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAA 597
           SATDNMPGWIPGNLN+GHLDRF+FLLAALTAADLAVYILCAKWYKYIKFESREAD A
Sbjct: 541 SATDNMPGWIPGNLNKGHLDRFYFLLAALTAADLAVYILCAKWYKYIKFESREADVA 595

BLAST of MC05g0248 vs. NCBI nr
Match: XP_004133948.1 (protein NRT1/ PTR FAMILY 7.3 [Cucumis sativus] >KGN56654.1 hypothetical protein Csa_011680 [Cucumis sativus])

HSP 1 Score: 1107 bits (2862), Expect = 0.0
Identity = 554/597 (92.80%), Postives = 572/597 (95.81%), Query Frame = 0

Query: 1   MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ 60
           MACL+ SCF Q+  +K   K   SEKE  TLDGAVDRHGHPAIRDKTGTW +GILILVNQ
Sbjct: 1   MACLSISCFDQSTKKK---KVMTSEKEVRTLDGAVDRHGHPAIRDKTGTWVSGILILVNQ 60

Query: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120
           GLATLAFFGVGVNLVLFLTRVVGQDNA AANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT
Sbjct: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120

Query: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGG 180
           CAIFQAIFV+GLASLSITSYIFLVRPKGCGDE TPCNSHSS H+ALFYLSVYLVALGNGG
Sbjct: 121 CAIFQAIFVVGLASLSITSYIFLVRPKGCGDEHTPCNSHSSLHIALFYLSVYLVALGNGG 180

Query: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240
           YQPNIATFGADQFDEEDPKEG SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW
Sbjct: 181 YQPNIATFGADQFDEEDPKEGQSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240

Query: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDD 300
           ASTASAA+ALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQ++ +SEGLFD+DD
Sbjct: 241 ASTASAALALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQMIPNSEGLFDDDD 300

Query: 301 KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLL 360
           KEL SNGAR+ILHTNGFKFLD+AAVITSSE DQL+DGARNPWRLCTVTQVEEVKCILRLL
Sbjct: 301 KELASNGARRILHTNGFKFLDKAAVITSSEYDQLDDGARNPWRLCTVTQVEEVKCILRLL 360

Query: 361 PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI 420
           PIWLCTILYSVVFTQMASLFV QGAAMRTNISTFH+PPASMSSFDILSVAAFIFIYRRVI
Sbjct: 361 PIWLCTILYSVVFTQMASLFVVQGAAMRTNISTFHVPPASMSSFDILSVAAFIFIYRRVI 420

Query: 421 DPVYARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFW 480
           DPV+ARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHA +DCL CD SSSLSIFW
Sbjct: 421 DPVFARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHANKDCLRCDNSSSLSIFW 480

Query: 481 QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI 540
           QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI
Sbjct: 481 QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI 540

Query: 541 SATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAA 597
           SATDNMPGWIPGNLNRGHLDRF+FLLAALTAADL VYILCAKWYKYIKFESREADAA
Sbjct: 541 SATDNMPGWIPGNLNRGHLDRFYFLLAALTAADLVVYILCAKWYKYIKFESREADAA 594

BLAST of MC05g0248 vs. NCBI nr
Match: XP_038878094.1 (protein NRT1/ PTR FAMILY 7.3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1105 bits (2857), Expect = 0.0
Identity = 547/575 (95.13%), Postives = 565/575 (98.26%), Query Frame = 0

Query: 23  ISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVV 82
           I EKE CTLDGAVDRHG PAIRDKTGTW AGILILVNQGLATLAFFGVGVNLVLFLTRVV
Sbjct: 3   IPEKEACTLDGAVDRHGRPAIRDKTGTWVAGILILVNQGLATLAFFGVGVNLVLFLTRVV 62

Query: 83  GQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIF 142
           GQDNA AANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIF
Sbjct: 63  GQDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIF 122

Query: 143 LVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGL 202
           LVRPKGCGDE TPCNSHSSFH+ALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEG+
Sbjct: 123 LVRPKGCGDEHTPCNSHSSFHIALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGI 182

Query: 203 SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRH 262
           SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRH
Sbjct: 183 SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRH 242

Query: 263 FTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDR 322
           FTPKGNPLSRVSQVV+AATRKWKVQ+M +SEGLFD+D+KELV+NGAR+ILHTNGFKFLDR
Sbjct: 243 FTPKGNPLSRVSQVVVAATRKWKVQMMPNSEGLFDDDEKELVNNGARRILHTNGFKFLDR 302

Query: 323 AAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVE 382
           AAVITSSE+D+L+DGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVE
Sbjct: 303 AAVITSSETDRLDDGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVE 362

Query: 383 QGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPVYARLTKSSLTELQRMGIGL 442
           QGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPV+ARLTKSSLTELQRMGIGL
Sbjct: 363 QGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPVFARLTKSSLTELQRMGIGL 422

Query: 443 VIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFWQIPQYVLIGASEVFMYVGQLEF 502
           VIAICAMVSAGTVEIFRLKHA ++CLHCD SSSLSIFWQIPQYVLIGASEVFMYVGQLEF
Sbjct: 423 VIAICAMVSAGTVEIFRLKHANKECLHCDDSSSLSIFWQIPQYVLIGASEVFMYVGQLEF 482

Query: 503 FNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLNRGHLDRF 562
           FNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMK+SATDNMPGWIPGNLN+GHLDRF
Sbjct: 483 FNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKVSATDNMPGWIPGNLNKGHLDRF 542

Query: 563 FFLLAALTAADLAVYILCAKWYKYIKFESREADAA 597
           +FLLAALTAADLAVYILCAKWYKYIKFESREAD A
Sbjct: 543 YFLLAALTAADLAVYILCAKWYKYIKFESREADVA 577

BLAST of MC05g0248 vs. NCBI nr
Match: KAA0049084.1 (protein NRT1/ PTR FAMILY 7.3-like [Cucumis melo var. makuwa] >TYK17479.1 protein NRT1/ PTR FAMILY 7.3-like [Cucumis melo var. makuwa])

HSP 1 Score: 1098 bits (2839), Expect = 0.0
Identity = 544/573 (94.94%), Postives = 561/573 (97.91%), Query Frame = 0

Query: 24  SEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVVG 83
           SEKE CTLDGAVDRHG PAIRDKTGTW +GILILVNQGLATLAFFGVGVNLVLFLTRV+G
Sbjct: 3   SEKEVCTLDGAVDRHGRPAIRDKTGTWVSGILILVNQGLATLAFFGVGVNLVLFLTRVLG 62

Query: 84  QDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIFL 143
           QDNA AANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSY+FL
Sbjct: 63  QDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYVFL 122

Query: 144 VRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLS 203
           VRPKGCGDE TPCNSHSS H+ALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEG S
Sbjct: 123 VRPKGCGDEHTPCNSHSSLHIALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGHS 182

Query: 204 KIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRHF 263
           KIAFFSYFYLALNLGSLFSNTILGYFEDEGMWV+GFWASTASAAMALLLFLCGIPRYRHF
Sbjct: 183 KIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGFWASTASAAMALLLFLCGIPRYRHF 242

Query: 264 TPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDRA 323
           TPKGNPLSRVSQVVIAATRKWKVQ+M +SEGLFD+DDKEL SNGAR+ILHTNGF+FLD+A
Sbjct: 243 TPKGNPLSRVSQVVIAATRKWKVQMMPNSEGLFDDDDKELASNGARRILHTNGFRFLDKA 302

Query: 324 AVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVEQ 383
           AVITSSE D+L+DGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVEQ
Sbjct: 303 AVITSSECDKLDDGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVEQ 362

Query: 384 GAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPVYARLTKSSLTELQRMGIGLV 443
           GAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPV+ARLTKSSLTELQRMGIGLV
Sbjct: 363 GAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPVFARLTKSSLTELQRMGIGLV 422

Query: 444 IAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFWQIPQYVLIGASEVFMYVGQLEFF 503
           IAICAMVSAGTVEIFRLKHA +DCLHCD SSSLSIFWQIPQYVLIGASEVFMYVGQLEFF
Sbjct: 423 IAICAMVSAGTVEIFRLKHANKDCLHCDDSSSLSIFWQIPQYVLIGASEVFMYVGQLEFF 482

Query: 504 NSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLNRGHLDRFF 563
           NSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLN+GHLDRF+
Sbjct: 483 NSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLNKGHLDRFY 542

Query: 564 FLLAALTAADLAVYILCAKWYKYIKFESREADA 596
           FLLAALTAADLAVYILCAKWYKYIKFESREADA
Sbjct: 543 FLLAALTAADLAVYILCAKWYKYIKFESREADA 575

BLAST of MC05g0248 vs. ExPASy TrEMBL
Match: A0A6J1CZL9 (protein NRT1/ PTR FAMILY 7.3-like OS=Momordica charantia OX=3673 GN=LOC111016031 PE=3 SV=1)

HSP 1 Score: 1187 bits (3071), Expect = 0.0
Identity = 595/598 (99.50%), Postives = 596/598 (99.67%), Query Frame = 0

Query: 1   MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ 60
           MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ
Sbjct: 1   MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ 60

Query: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120
           GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT
Sbjct: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120

Query: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGG 180
           CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNS SSFHMALFYLSVYLVALGNGG
Sbjct: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSRSSFHMALFYLSVYLVALGNGG 180

Query: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240
           YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW
Sbjct: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240

Query: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDD 300
           ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAAT KWKVQIMTDSEGLFDEDD
Sbjct: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATTKWKVQIMTDSEGLFDEDD 300

Query: 301 KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLL 360
           KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLL
Sbjct: 301 KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLL 360

Query: 361 PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI 420
           PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI
Sbjct: 361 PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI 420

Query: 421 DPVYARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFW 480
           DPVYARLTKSS+TELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFW
Sbjct: 421 DPVYARLTKSSVTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFW 480

Query: 481 QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI 540
           QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI
Sbjct: 481 QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI 540

Query: 541 SATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAAV 598
           SATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAAV
Sbjct: 541 SATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAAV 598

BLAST of MC05g0248 vs. ExPASy TrEMBL
Match: A0A0A0L6J7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G127170 PE=3 SV=1)

HSP 1 Score: 1107 bits (2862), Expect = 0.0
Identity = 554/597 (92.80%), Postives = 572/597 (95.81%), Query Frame = 0

Query: 1   MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ 60
           MACL+ SCF Q+  +K   K   SEKE  TLDGAVDRHGHPAIRDKTGTW +GILILVNQ
Sbjct: 1   MACLSISCFDQSTKKK---KVMTSEKEVRTLDGAVDRHGHPAIRDKTGTWVSGILILVNQ 60

Query: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120
           GLATLAFFGVGVNLVLFLTRVVGQDNA AANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT
Sbjct: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120

Query: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGG 180
           CAIFQAIFV+GLASLSITSYIFLVRPKGCGDE TPCNSHSS H+ALFYLSVYLVALGNGG
Sbjct: 121 CAIFQAIFVVGLASLSITSYIFLVRPKGCGDEHTPCNSHSSLHIALFYLSVYLVALGNGG 180

Query: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240
           YQPNIATFGADQFDEEDPKEG SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW
Sbjct: 181 YQPNIATFGADQFDEEDPKEGQSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240

Query: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDD 300
           ASTASAA+ALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQ++ +SEGLFD+DD
Sbjct: 241 ASTASAALALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQMIPNSEGLFDDDD 300

Query: 301 KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLL 360
           KEL SNGAR+ILHTNGFKFLD+AAVITSSE DQL+DGARNPWRLCTVTQVEEVKCILRLL
Sbjct: 301 KELASNGARRILHTNGFKFLDKAAVITSSEYDQLDDGARNPWRLCTVTQVEEVKCILRLL 360

Query: 361 PIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVI 420
           PIWLCTILYSVVFTQMASLFV QGAAMRTNISTFH+PPASMSSFDILSVAAFIFIYRRVI
Sbjct: 361 PIWLCTILYSVVFTQMASLFVVQGAAMRTNISTFHVPPASMSSFDILSVAAFIFIYRRVI 420

Query: 421 DPVYARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFW 480
           DPV+ARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHA +DCL CD SSSLSIFW
Sbjct: 421 DPVFARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHANKDCLRCDNSSSLSIFW 480

Query: 481 QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI 540
           QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI
Sbjct: 481 QIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKI 540

Query: 541 SATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAA 597
           SATDNMPGWIPGNLNRGHLDRF+FLLAALTAADL VYILCAKWYKYIKFESREADAA
Sbjct: 541 SATDNMPGWIPGNLNRGHLDRFYFLLAALTAADLVVYILCAKWYKYIKFESREADAA 594

BLAST of MC05g0248 vs. ExPASy TrEMBL
Match: A0A5D3D3J4 (Protein NRT1/ PTR FAMILY 7.3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003440 PE=3 SV=1)

HSP 1 Score: 1098 bits (2839), Expect = 0.0
Identity = 544/573 (94.94%), Postives = 561/573 (97.91%), Query Frame = 0

Query: 24  SEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVVG 83
           SEKE CTLDGAVDRHG PAIRDKTGTW +GILILVNQGLATLAFFGVGVNLVLFLTRV+G
Sbjct: 3   SEKEVCTLDGAVDRHGRPAIRDKTGTWVSGILILVNQGLATLAFFGVGVNLVLFLTRVLG 62

Query: 84  QDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIFL 143
           QDNA AANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSY+FL
Sbjct: 63  QDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYVFL 122

Query: 144 VRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLS 203
           VRPKGCGDE TPCNSHSS H+ALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEG S
Sbjct: 123 VRPKGCGDEHTPCNSHSSLHIALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGHS 182

Query: 204 KIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRHF 263
           KIAFFSYFYLALNLGSLFSNTILGYFEDEGMWV+GFWASTASAAMALLLFLCGIPRYRHF
Sbjct: 183 KIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGFWASTASAAMALLLFLCGIPRYRHF 242

Query: 264 TPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDRA 323
           TPKGNPLSRVSQVVIAATRKWKVQ+M +SEGLFD+DDKEL SNGAR+ILHTNGF+FLD+A
Sbjct: 243 TPKGNPLSRVSQVVIAATRKWKVQMMPNSEGLFDDDDKELASNGARRILHTNGFRFLDKA 302

Query: 324 AVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVEQ 383
           AVITSSE D+L+DGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVEQ
Sbjct: 303 AVITSSECDKLDDGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVEQ 362

Query: 384 GAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPVYARLTKSSLTELQRMGIGLV 443
           GAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPV+ARLTKSSLTELQRMGIGLV
Sbjct: 363 GAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPVFARLTKSSLTELQRMGIGLV 422

Query: 444 IAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFWQIPQYVLIGASEVFMYVGQLEFF 503
           IAICAMVSAGTVEIFRLKHA +DCLHCD SSSLSIFWQIPQYVLIGASEVFMYVGQLEFF
Sbjct: 423 IAICAMVSAGTVEIFRLKHANKDCLHCDDSSSLSIFWQIPQYVLIGASEVFMYVGQLEFF 482

Query: 504 NSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLNRGHLDRFF 563
           NSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLN+GHLDRF+
Sbjct: 483 NSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLNKGHLDRFY 542

Query: 564 FLLAALTAADLAVYILCAKWYKYIKFESREADA 596
           FLLAALTAADLAVYILCAKWYKYIKFESREADA
Sbjct: 543 FLLAALTAADLAVYILCAKWYKYIKFESREADA 575

BLAST of MC05g0248 vs. ExPASy TrEMBL
Match: A0A6J1IQW8 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita maxima OX=3661 GN=LOC111479679 PE=3 SV=1)

HSP 1 Score: 1081 bits (2795), Expect = 0.0
Identity = 535/598 (89.46%), Postives = 565/598 (94.48%), Query Frame = 0

Query: 1   MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ 60
           MACLN SCFRQ+  E+  K  T +EKE  TLDGAVDRHG PA+R KTGTW AGIL+LVNQ
Sbjct: 1   MACLNISCFRQSTMERRKKTMTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVNQ 60

Query: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120
           GLATLAFFGVGVNLVLFLTRV+GQDNAAAANNVSKWTGTVYIFSLLGAFLSDSY GRFKT
Sbjct: 61  GLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFKT 120

Query: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGG 180
           CAIFQAIFV+GLASLSITS +FL++PKGCGDE TPC+SHSSFH+ALFYLSVYLVALGNGG
Sbjct: 121 CAIFQAIFVLGLASLSITSDLFLLKPKGCGDEHTPCDSHSSFHIALFYLSVYLVALGNGG 180

Query: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240
           YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWV+GFW
Sbjct: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGFW 240

Query: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDD 300
           ASTASAAMAL+LFLCGIPRYRHFTPKGNPLSRVSQV++AATRKWKVQIM +SEGLFDED+
Sbjct: 241 ASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDEDE 300

Query: 301 KELVSN-GARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRL 360
           K+LVSN GARKILHTNGF+FLDRAA ITS E DQ +DG RNPWRLCTVTQVEEVKC+LRL
Sbjct: 301 KDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFDDGTRNPWRLCTVTQVEEVKCVLRL 360

Query: 361 LPIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRV 420
           LPIWLCTILYSVVFTQMASLFVEQGA MRTNISTFHIPPASMSSFDILSVAAFIFIYRRV
Sbjct: 361 LPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRRV 420

Query: 421 IDPVYARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIF 480
           IDP+Y RLTKSSLTELQRMGIGLVIAICAMVSAGTVE+FRL++A +DCLHC+ SSSLSIF
Sbjct: 421 IDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSIF 480

Query: 481 WQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMK 540
           WQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMK
Sbjct: 481 WQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMK 540

Query: 541 ISATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAA 597
           +S   NMPGWIPGNLNRGHLDRFFFLLA LTAADL VYI+CAKWYKYIKFESREA AA
Sbjct: 541 VSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA 598

BLAST of MC05g0248 vs. ExPASy TrEMBL
Match: A0A6J1FGI5 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita moschata OX=3662 GN=LOC111443982 PE=3 SV=1)

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 536/599 (89.48%), Postives = 564/599 (94.16%), Query Frame = 0

Query: 1   MACLNTSCFRQTVGEKTT--KKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILV 60
           M CLN       + + TT  KK  + EKE CTLDGAVDRHG PAIRDKTGTW AGILILV
Sbjct: 1   MPCLN-------ISKSTTEMKKTVMPEKEACTLDGAVDRHGCPAIRDKTGTWVAGILILV 60

Query: 61  NQGLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRF 120
           NQGLATLAFFGVGVNLVLFLTRV+GQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRF
Sbjct: 61  NQGLATLAFFGVGVNLVLFLTRVLGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRF 120

Query: 121 KTCAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGN 180
           KTCAIFQAIFV+GLASLSITSYIFLVRPKGCGDE TPCN+HSSFH+ALFYLSVYLVALGN
Sbjct: 121 KTCAIFQAIFVLGLASLSITSYIFLVRPKGCGDEHTPCNAHSSFHIALFYLSVYLVALGN 180

Query: 181 GGYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLG 240
           GGYQPNIATFGADQFDEED KEG+SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLG
Sbjct: 181 GGYQPNIATFGADQFDEEDAKEGISKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLG 240

Query: 241 FWASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDE 300
           FWASTASAAMAL+LFLCGIPRYRHFTPKGNPLSRVSQVV+AA RKWKVQIM DSEGLFD+
Sbjct: 241 FWASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVVVAAIRKWKVQIMNDSEGLFDD 300

Query: 301 DDKELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILR 360
           D+KELV NGARKILHTNGFKFLD+AAV+TS+E ++ + G RNPWRLCTVTQVEEVKCI+R
Sbjct: 301 DEKELVGNGARKILHTNGFKFLDKAAVVTSAEMERFDGGDRNPWRLCTVTQVEEVKCIIR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFVEQGAAMRTNIS+FHIPPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVEQGAAMRTNISSFHIPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPVYARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSI 480
           +IDP + RLTK+SLTELQRMGIGLVIA+CAM+ AGTVEIFRLK+A +DC HCD SSSLSI
Sbjct: 421 IIDPTFVRLTKTSLTELQRMGIGLVIAVCAMIFAGTVEIFRLKYANKDCRHCDDSSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KISATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAA 597
           KIS TDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESR+A AA
Sbjct: 541 KISTTDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESRDAAAA 592

BLAST of MC05g0248 vs. TAIR 10
Match: AT1G32450.1 (nitrate transporter 1.5 )

HSP 1 Score: 841.6 bits (2173), Expect = 3.8e-244
Identity = 421/599 (70.28%), Postives = 489/599 (81.64%), Query Frame = 0

Query: 1   MACLNTSCFRQTVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQ 60
           M+CL        +  K T K+   E+E  T DG VD +G P+IR  +G W AGI+IL+NQ
Sbjct: 1   MSCLE-------IYNKDTMKKKEGEEE--TRDGTVDYYGRPSIRSNSGQWVAGIVILLNQ 60

Query: 61  GLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKT 120
           GLATLAFFGVGVNLVLFLTRV+ Q+NA AANNVSKWTGTVYIFSL+GAFLSDSYWGR+KT
Sbjct: 61  GLATLAFFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKT 120

Query: 121 CAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGG 180
           CAIFQ IFVIGL+SLS++SY+FL+RP+GCGDE TPC SHS   + +FY S+YL+ALG GG
Sbjct: 121 CAIFQVIFVIGLSSLSLSSYMFLIRPRGCGDEVTPCGSHSMMEITMFYFSIYLIALGYGG 180

Query: 181 YQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFW 240
           YQPNIAT GADQFDEE PKEG SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMW LGFW
Sbjct: 181 YQPNIATLGADQFDEEHPKEGYSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWALGFW 240

Query: 241 ASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQI-MTDSEGLFDED 300
           AST SA + L+LFL G PRYR+F P GNPLSR  QV++AAT+K  V+  +   E ++D D
Sbjct: 241 ASTGSAIIGLILFLVGTPRYRYFKPTGNPLSRFCQVLVAATKKSSVEAPLRGREEMYDGD 300

Query: 301 D--KELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCIL 360
              K    N  R+I+HT+ FKFLD+AA IT+ + D  +  + NPWRLC VTQVEEVKCIL
Sbjct: 301 SEGKNASVNTGRRIVHTDEFKFLDKAAYITARDLDDKKQDSVNPWRLCPVTQVEEVKCIL 360

Query: 361 RLLPIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYR 420
           RL+PIWLCTI+YSVVFTQMASLFVEQGAAM T++S F IPPASMSSFDILSVA FIF+YR
Sbjct: 361 RLMPIWLCTIIYSVVFTQMASLFVEQGAAMNTSVSDFKIPPASMSSFDILSVALFIFLYR 420

Query: 421 RVIDPVYARLTKS---SLTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSS 480
           RV++PV  R  K+    +TEL RMGIGLVIA+ AM++AG VE +RLK+A + C HCDGSS
Sbjct: 421 RVLEPVANRFKKNGSKGITELHRMGIGLVIAVIAMIAAGIVECYRLKYADKSCTHCDGSS 480

Query: 481 SLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLV 540
           SLSIFWQ PQY LIGASEVFMYVGQLEFFN+Q PDGLKSFGSALCM S+S GN+VSSLLV
Sbjct: 481 SLSIFWQAPQYSLIGASEVFMYVGQLEFFNAQTPDGLKSFGSALCMMSMSMGNFVSSLLV 540

Query: 541 TIVMKISATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESRE 594
           T+V+KIS  D+MPGWIP NLN+GHLDRF+FLLAALT+ DL VYI CAKWYK I+ E ++
Sbjct: 541 TMVVKISTEDHMPGWIPRNLNKGHLDRFYFLLAALTSIDLVVYIACAKWYKPIQLEGKD 590

BLAST of MC05g0248 vs. TAIR 10
Match: AT4G21680.1 (NITRATE TRANSPORTER 1.8 )

HSP 1 Score: 815.1 bits (2104), Expect = 3.8e-236
Identity = 412/576 (71.53%), Postives = 470/576 (81.60%), Query Frame = 0

Query: 23  ISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVV 82
           + + E CT DG+VDRHG+PAIR  TG W   ILILVNQGLATLAFFGVGVNLVLFLTRV+
Sbjct: 5   VRQFEVCTQDGSVDRHGNPAIRANTGKWLTAILILVNQGLATLAFFGVGVNLVLFLTRVM 64

Query: 83  GQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIF 142
           GQDNA AANNVSKWTGTVYIFSLLGAFLSDSYWGR+KTCAIFQA FV GL  LS+++   
Sbjct: 65  GQDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRYKTCAIFQASFVAGLMMLSLSTGAL 124

Query: 143 LVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGL 202
           L+ P GCG E +PC  HS+F   LFYLSVYL+ALG GGYQPNIATFGADQFD ED  EG 
Sbjct: 125 LLEPSGCGVEDSPCKPHSTFKTVLFYLSVYLIALGYGGYQPNIATFGADQFDAEDSVEGH 184

Query: 203 SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRH 262
           SKIAFFSYFYLALNLGSLFSNT+LGYFED+G W LGFWAS  SA   L+LFL G P+YRH
Sbjct: 185 SKIAFFSYFYLALNLGSLFSNTVLGYFEDQGEWPLGFWASAGSAFAGLVLFLIGTPKYRH 244

Query: 263 FTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDR 322
           FTP+ +P SR  QV++AATRK K+ +  +   L+   D E    G +KILHT GF+FLDR
Sbjct: 245 FTPRESPWSRFCQVLVAATRKAKIDVHHEELNLY---DSETQYTGDKKILHTKGFRFLDR 304

Query: 323 AAVIT-SSESDQLEDGAR-NPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLF 382
           AA++T   E++++E G++ +PWRLC+VTQVEEVKC+LRLLPIWLCTILYSVVFTQMASLF
Sbjct: 305 AAIVTPDDEAEKVESGSKYDPWRLCSVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLF 364

Query: 383 VEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPVYARLTKS----SLTELQ 442
           V QGAAM+TNI  F IP +SMSSFDILSVA FIF YRR +DP++ARL K+     LTELQ
Sbjct: 365 VVQGAAMKTNIKNFRIPASSMSSFDILSVAFFIFAYRRFLDPLFARLNKTERNKGLTELQ 424

Query: 443 RMGIGLVIAICAMVSAGTVEIFRLKH-ATRDCLHCDGSSSLSIFWQIPQYVLIGASEVFM 502
           RMGIGLVIAI AM+SAG VEI RLK+           SS+LSIFWQ+PQY+LIGASEVFM
Sbjct: 425 RMGIGLVIAIMAMISAGIVEIHRLKNKEPESATSISSSSTLSIFWQVPQYMLIGASEVFM 484

Query: 503 YVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLN 562
           YVGQLEFFNSQAP GLKSF SALCM SIS GNYVSSLLV+IVMKIS TD++ GWIP NLN
Sbjct: 485 YVGQLEFFNSQAPTGLKSFASALCMASISLGNYVSSLLVSIVMKISTTDDVHGWIPENLN 544

Query: 563 RGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFES 592
           +GHL+RF+FLLA LTAAD  VY++CAKWYKYIK E+
Sbjct: 545 KGHLERFYFLLAGLTAADFVVYLICAKWYKYIKSEA 577

BLAST of MC05g0248 vs. TAIR 10
Match: AT5G19640.1 (Major facilitator superfamily protein )

HSP 1 Score: 646.4 bits (1666), Expect = 2.3e-185
Identity = 337/547 (61.61%), Postives = 398/547 (72.76%), Query Frame = 0

Query: 46  KTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSL 105
           K G W   I++LVNQGLATLAFFGVGVNLVLFLTRV+GQ NA AANNVSKWTGTVY+FSL
Sbjct: 59  KNGGWTNAIILLVNQGLATLAFFGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFSL 118

Query: 106 LGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSHSSFHMA 165
           +GAFLSDSYWGR+ TC IFQ IFVIG+  LS  S+ FL++P+GCGD    CN  SS  +A
Sbjct: 119 VGAFLSDSYWGRYLTCTIFQVIFVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGVA 178

Query: 166 LFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTI 225
           +FYLSVYLVA G GG+QP +ATFGADQ D++      SK AFFSYFY ALN+G+LFSNTI
Sbjct: 179 IFYLSVYLVAFGYGGHQPTLATFGADQLDDDKN----SKAAFFSYFYFALNVGALFSNTI 238

Query: 226 LGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWK 285
           L YFED+G+W  GF  S  SA +AL+ FL    +YR+  P GNPL RV+QV +A  RKW 
Sbjct: 239 LVYFEDKGLWTEGFLVSLGSAIVALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKWS 298

Query: 286 VQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDRAAVITSSESDQLEDGAR-NPWRL 345
           V    D   L++ +  E    G+RKI H+  F FLDRAAVIT ++     +G R N WRL
Sbjct: 299 VVRPGDPHELYELEGPESAIKGSRKIFHSTKFLFLDRAAVITEND----RNGTRSNAWRL 358

Query: 346 CTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSF 405
           C+VTQVEE KC+++LLPIWLCTI+YSV+FTQMASLFVEQG  M   +  FHIP ASMS F
Sbjct: 359 CSVTQVEEAKCVMKLLPIWLCTIIYSVIFTQMASLFVEQGDVMNAYVGKFHIPAASMSVF 418

Query: 406 DILSVAAFIFIYRRVIDPVYARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHAT 465
           DI SV     IYR +I P Y R      TEL RMGIGL+I I AMV+AG  EI RLK   
Sbjct: 419 DIFSVFVSTGIYRHIIFP-YVR-----PTELMRMGIGLIIGIMAMVAAGLTEIQRLKRVV 478

Query: 466 RDCLHCDGSSSLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSIS 525
                    S L+I WQIPQYVL+GASEVFMYVGQLEFFN QAPDGLK+ GS+LCM S++
Sbjct: 479 PG----QKESELTILWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMASMA 538

Query: 526 FGNYVSSLLVTIVMKISAT-DNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKW 585
            GNYVSSL+V IVM I+   +N PGWIP NLN GH+DRF+FL+AAL A D  VY++ AKW
Sbjct: 539 LGNYVSSLMVNIVMAITKRGENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIFAKW 587

Query: 586 YKYIKFE 591
           Y+ I  +
Sbjct: 599 YQPISHD 587

BLAST of MC05g0248 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 498.8 bits (1283), Expect = 6.0e-141
Identity = 257/573 (44.85%), Postives = 383/573 (66.84%), Query Frame = 0

Query: 23  ISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVV 82
           + EK+  T DG VD H +PA ++KTG W A   IL N+    LA++G+G NLV +L   +
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRL 60

Query: 83  GQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIF 142
            Q NA AANNV+ W+GT YI  L+GAF++D+Y GR+ T A F  I+V G+  L++++ + 
Sbjct: 61  NQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVP 120

Query: 143 LVRPKGCGDEQTPCNSHSSFHMALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGL 202
            ++P  C  +   C+ +SS   A+F++++Y++ALG GG +P +++FGADQFDE D  E +
Sbjct: 121 GLKPGNCNAD--TCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKI 180

Query: 203 SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRH 262
            K +FF++FY ++N+G+L + T+L + +    W  GF   T +  +A+  F  G   YR 
Sbjct: 181 KKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRL 240

Query: 263 FTPKGNPLSRVSQVVIAATRKWKVQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDR 322
             P G+PL+R+ QV++AA RK  V++  D   LF+  D E    G+RK++HT+  KF D+
Sbjct: 241 QRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDK 300

Query: 323 AAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVE 382
           AAV   S+SD ++DG  NPWRLC+VTQVEE+K I+ LLP+W   I+++ V++QM+++FV 
Sbjct: 301 AAV--ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVL 360

Query: 383 QGAAMRTNI-STFHIPPASMSSFDILSVAAFIFIYRRVIDPVYARLTKS--SLTELQRMG 442
           QG  M  ++   F IP AS+S FD +SV  +  +Y + I P+  + T++    T+LQRMG
Sbjct: 361 QGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMG 420

Query: 443 IGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSIFWQIPQYVLIGASEVFMYVGQ 502
           IGLV++I AM++AG +E+ RL +      +      +SIFWQIPQY+LIG +EVF ++GQ
Sbjct: 421 IGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQ 480

Query: 503 LEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLNRGHL 562
           LEFF  QAPD ++S  SAL +T+++ GNY+S++LVT+VMKI+  +  PGWIP NLNRGHL
Sbjct: 481 LEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHL 540

Query: 563 DRFFFLLAALTAADLAVYILCAKWYKYIKFESR 593
           D FF+LLA L+  +  VY+  +K YKY K   R
Sbjct: 541 DYFFYLLATLSFLNFLVYLWISKRYKYKKAVGR 568

BLAST of MC05g0248 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 471.1 bits (1211), Expect = 1.3e-132
Identity = 240/565 (42.48%), Postives = 365/565 (64.60%), Query Frame = 0

Query: 32  DGAVDRHGHPAIRDKTGTWFAGILILVNQGLATLAFFGVGVNLVLFLTRVVGQDNAAAAN 91
           DG+VD +G+P +++KTG W A   IL N+    LA++G+  NL+ +LT  + Q N +AA 
Sbjct: 27  DGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAAT 86

Query: 92  NVSKWTGTVYIFSLLGAFLSDSYWGRFKTCAIFQAIFVIGLASLSITSYIFLVRPKGC-G 151
           NV+ W GT Y+  L+GA L+D+YWGR+ T A F  I+ IG+++L++++ +  ++P  C G
Sbjct: 87  NVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECIG 146

Query: 152 DEQTPCNSHSSFHMALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSKIAFFSY 211
           D    C S +    A+F+  +YL+ALG GG +P +++FGADQFD+ D +E + K +FF++
Sbjct: 147 DF---CPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNW 206

Query: 212 FYLALNLGSLFSNTILGYFEDEGMWVLGFWASTASAAMALLLFLCGIPRYRHFTPKGNPL 271
           FY ++N+G+L S+++L + ++   W LGF   T    +A+  F  G P YR   P G+P+
Sbjct: 207 FYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPI 266

Query: 272 SRVSQVVIAATRKWKVQIMTDSEGLFDEDDKELVSNGARKILHTNGFKFLDRAAVITSSE 331
           +R+SQVV+A+ RK  V++  D+  L++  DK     G+RKI HT+  ++LD+AAVI  SE
Sbjct: 267 TRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVI--SE 326

Query: 332 SDQLEDGARNPWRLCTVTQVEEVKCILRLLPIWLCTILYSVVFTQMASLFVEQGAAMRTN 391
            +       N WRLCTVTQVEE+K ++R+ PIW   I++S V+ QM+++FV+QG AM   
Sbjct: 327 EESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCK 386

Query: 392 ISTFHIPPASMSSFDILSVAAFIFIYRRVIDPVYARLT--KSSLTELQRMGIGLVIAICA 451
           I +F +PPA++ +FD  SV  ++ +Y R I P+  + T      TE+QRMGIGL +++  
Sbjct: 387 IGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLC 446

Query: 452 MVSAGTVEIFRLKHATRD--CLHCDGSSSLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQ 511
           M +A  VEI RL H   D   +       +S+ WQIPQY ++GA+EVF ++GQLEFF  Q
Sbjct: 447 MAAAAIVEIIRL-HMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQ 506

Query: 512 APDGLKSFGSALCMTSISFGNYVSSLLVTIVMKISATDNMPGWIPGNLNRGHLDRFFFLL 571
           +PD ++S  SAL + + + GNY+SSL++T+V   +  +   GWI  NLN GHLD FF+LL
Sbjct: 507 SPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFWLL 566

Query: 572 AALTAADLAVYILCAKWYKYIKFES 592
           A L+  ++AVY   A  YK  K  S
Sbjct: 567 AGLSLVNMAVYFFSAARYKQKKASS 585