MC05g0230 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0230 vs. NCBI nr
Match: XP_008438268.1 (PREDICTED: uncharacterized protein LOC103483430 [Cucumis melo] >KAA0049104.1 uncharacterized protein E6C27_scaffold171G003340 [Cucumis melo var. makuwa] >TYK17459.1 uncharacterized protein E5676_scaffold434G003230 [Cucumis melo var. makuwa])

HSP 1 Score: 446 bits (1147), Expect = 2.62e-155
Identity = 234/310 (75.48%), Postives = 260/310 (83.87%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEK-QAELRSGAV---DDPLPPPLVF 60
           MRC+KH+SD +S++GVCASCLRERLL++IAAQAQAEK Q++L SG +   DDPLPPPL+F
Sbjct: 1   MRCKKHHSDFTSTVGVCASCLRERLLSIIAAQAQAEKNQSQLTSGGIRSADDPLPPPLLF 60

Query: 61  LRSVSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVTTGSF 120
           L SVSP+A   KS+ED+W++LDREGN R RHQRFYSTPQI PN RTNNSAN TFVTTGSF
Sbjct: 61  LHSVSPHA--TKSNEDLWTNLDREGNLRSRHQRFYSTPQIGPNCRTNNSANTTFVTTGSF 120

Query: 121 DQKPKSK-FSLWSKMFRSRSEKFEKNHKIPSRESRGPASYSSTPSWFSTIFAGRR-SNQS 180
           D+K +SK FSLWSK+FRSRSEKFEKNHK PSRES GP S SS+PSWFSTIF GRR   QS
Sbjct: 121 DRKQRSKKFSLWSKLFRSRSEKFEKNHKSPSRESHGPGSSSSSPSWFSTIFHGRRIKRQS 180

Query: 181 SLSTAEEPVAAAERRHCPVIERGMSPAGLSDSDEEHEGPDRSPISQK---SPMAAPGSAK 240
           SL+  EE +  AERRHC  IERGMSP  +SDSDEE EGPDRSPISQK   SPMAAPGSAK
Sbjct: 181 SLTPVEESIPVAERRHCQAIERGMSPVRVSDSDEECEGPDRSPISQKFQLSPMAAPGSAK 240

Query: 241 RGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVTPPDITFSGNLRASPKPHLCANRSRKI 300
           RGR GH QN SGFAFCLSPLVRASPN N NQKV PP+  FSGN++   KPHLCANRSRKI
Sbjct: 241 RGRLGHNQNVSGFAFCLSPLVRASPNRNWNQKVIPPETAFSGNIKVPAKPHLCANRSRKI 300

BLAST of MC05g0230 vs. NCBI nr
Match: XP_038898909.1 (uncharacterized protein LOC120086361 [Benincasa hispida])

HSP 1 Score: 443 bits (1140), Expect = 3.04e-154
Identity = 237/310 (76.45%), Postives = 259/310 (83.55%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEK-QAELRSG---AVDDPLPPPLVF 60
           MRC+KH+SD SS++GVCASCLRERLLT+IAAQAQ +K Q+EL SG   A DDPLP PLVF
Sbjct: 1   MRCKKHHSDFSSTVGVCASCLRERLLTIIAAQAQTDKNQSELSSGGIRAADDPLPSPLVF 60

Query: 61  LRSVSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVTTGSF 120
           LRSVSPYA   KSDED+WS+LD EGNRR RHQRFYSTPQI PN+RTNNSAN TFV+TGSF
Sbjct: 61  LRSVSPYA--GKSDEDLWSNLDPEGNRRPRHQRFYSTPQIGPNYRTNNSANTTFVSTGSF 120

Query: 121 DQKPKSK-FSLWSKMFRSRSEKFEKNHKIPSRESRGPASYSSTPSWFSTIFAGRR-SNQS 180
           D+K +SK FSLWSK+FRSRSE FEKNHK  SRES GP S SS+PSWFSTIF GRR   QS
Sbjct: 121 DRKQRSKKFSLWSKLFRSRSENFEKNHKNTSRESHGPDSSSSSPSWFSTIFNGRRIKQQS 180

Query: 181 SLSTAEEPVAAAERRHCPVIERGMSPAGLSDSDEEHEGPDRSPISQK---SPMAAPGSAK 240
           SL+  EE +A AERRHC  IERGMSP  +SDSDEE EG +RSPISQK   SPMAAPGSAK
Sbjct: 181 SLTPVEESIAVAERRHCQGIERGMSPVRVSDSDEECEGSNRSPISQKFQSSPMAAPGSAK 240

Query: 241 RGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVTPPDITFSGNLRASPKPHLCANRSRKI 300
           RGR GH QN SGFAFCLSPLVRASPN N NQKV PP+ +FSGN+R   KPHLCANRSRKI
Sbjct: 241 RGRLGHNQNVSGFAFCLSPLVRASPNRNWNQKVVPPETSFSGNVRVPAKPHLCANRSRKI 300

BLAST of MC05g0230 vs. NCBI nr
Match: XP_011652747.1 (uncharacterized protein LOC105435079 [Cucumis sativus] >KGN56673.1 hypothetical protein Csa_009477 [Cucumis sativus])

HSP 1 Score: 441 bits (1135), Expect = 1.75e-153
Identity = 231/310 (74.52%), Postives = 259/310 (83.55%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEK-QAELRSGAV---DDPLPPPLVF 60
           MRC+KH+SD +S++GVCASCLRERLL++IAAQAQAEK Q++L  G +   DDPLPPPL+F
Sbjct: 1   MRCKKHHSDFTSTVGVCASCLRERLLSIIAAQAQAEKNQSQLTYGGIRSADDPLPPPLLF 60

Query: 61  LRSVSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVTTGSF 120
           + SVSP+A   KSDED+WS+LDREGNRR  HQRFYSTPQI PN RTNNSAN TFVTTGSF
Sbjct: 61  IHSVSPHA--TKSDEDLWSNLDREGNRRFLHQRFYSTPQIGPNGRTNNSANTTFVTTGSF 120

Query: 121 DQKPKSK-FSLWSKMFRSRSEKFEKNHKIPSRESRGPASYSSTPSWFSTIFAGRRSN-QS 180
           D+K +SK FSLWSK+FRSRS+KFEKNHK PSRES GP S SS+PSWFSTIF G R+  QS
Sbjct: 121 DRKQRSKKFSLWSKLFRSRSDKFEKNHKSPSRESHGPGSSSSSPSWFSTIFHGHRTKRQS 180

Query: 181 SLSTAEEPVAAAERRHCPVIERGMSPAGLSDSDEEHEGPDRSPISQK---SPMAAPGSAK 240
           SLS  EE ++ AERRHC  IERGMSP  +SDSDEE EGPDRSPISQK   SPMAAPGSAK
Sbjct: 181 SLSPVEESISVAERRHCHAIERGMSPVRVSDSDEECEGPDRSPISQKFQLSPMAAPGSAK 240

Query: 241 RGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVTPPDITFSGNLRASPKPHLCANRSRKI 300
           RGR GH QN SGFAFCLSPL+RASPN N NQK  PP+  FSGN++   KPHLCANRSRKI
Sbjct: 241 RGRLGHNQNVSGFAFCLSPLMRASPNRNWNQKAIPPETAFSGNIKVPAKPHLCANRSRKI 300

BLAST of MC05g0230 vs. NCBI nr
Match: KAG6597247.1 (hypothetical protein SDJN03_10427, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028716.1 hypothetical protein SDJN02_09897, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 423 bits (1088), Expect = 3.18e-146
Identity = 229/315 (72.70%), Postives = 252/315 (80.00%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEKQAELRSGAVDD-------PLPPP 60
           MRC+KH  DCSSSIGVCASCLRERL  +IAAQAQAEKQ+E+R+ A  D       PLPPP
Sbjct: 1   MRCKKHQFDCSSSIGVCASCLRERLFLIIAAQAQAEKQSEIRAAASGDNRAADDRPLPPP 60

Query: 61  -LVFLRSVSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVT 120
            LVFLRSVSPY  R KSDE + SDLDREGNRR RHQRFYSTPQI P++RTNN+AN TFVT
Sbjct: 61  PLVFLRSVSPYDHRLKSDEGLLSDLDREGNRRNRHQRFYSTPQIGPDYRTNNTANSTFVT 120

Query: 121 TGSFDQKPKSKFSLWSKMFRSRSEKFEKNHKIPSRESRGPASYSSTPSWFSTIFAGRR-S 180
           TGSFD+K +SKFSLWSK+F+SRSEKFEK HK PS ES G  S S +PSWFSTI  GRR  
Sbjct: 121 TGSFDRKKRSKFSLWSKLFKSRSEKFEKKHKSPSHESHGSDSASLSPSWFSTILNGRRIK 180

Query: 181 NQSSLSTAEEPVAAAERRH-CPVIERGMSPAGLSDSDEEH-EGPDRSPISQK---SPMAA 240
            QSSL+T EE +A AERRH C  I+RGMSPA +S+SDEE  E   RSPISQK   SPM  
Sbjct: 181 QQSSLATVEESIAGAERRHHCQTIKRGMSPAIISESDEEECESHGRSPISQKFQQSPMTI 240

Query: 241 PGSAKRGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVTPPDITFSGNLRASPKPHLCAN 300
           PGSAKR + GHKQN SGFAFCLSPLVRASPN N NQKV PP+++FSGNLR   KPHLCAN
Sbjct: 241 PGSAKREKLGHKQNVSGFAFCLSPLVRASPNRNWNQKVMPPEVSFSGNLRVPGKPHLCAN 300

BLAST of MC05g0230 vs. NCBI nr
Match: XP_022974739.1 (uncharacterized protein LOC111473474 [Cucurbita maxima] >XP_022975245.1 uncharacterized protein LOC111474358 [Cucurbita maxima])

HSP 1 Score: 421 bits (1082), Expect = 2.60e-145
Identity = 228/315 (72.38%), Postives = 251/315 (79.68%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEKQAELRSGAVDD-------PLPPP 60
           MRC+KH  DCSSSIGVCASCLRERL  +IAAQAQAEKQ+E+R+ A  D       PLPPP
Sbjct: 1   MRCKKHQFDCSSSIGVCASCLRERLFLIIAAQAQAEKQSEIRAAAAGDNRAADDRPLPPP 60

Query: 61  -LVFLRSVSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVT 120
            LVFLRSVSPY  R KSDE + SDLDREGNRR RHQRFYSTPQI P++RTNN+AN TFVT
Sbjct: 61  PLVFLRSVSPYDHRLKSDEGLLSDLDREGNRRNRHQRFYSTPQIGPDYRTNNTANSTFVT 120

Query: 121 TGSFDQKPKSKFSLWSKMFRSRSEKFEKNHKIPSRESRGPASYSSTPSWFSTIFAGRR-S 180
           TGSFD+K +SKFSLWSK+F+SRSEKFEK HK PS ES G  S S +PSWFSTI  GRR  
Sbjct: 121 TGSFDRKKRSKFSLWSKLFKSRSEKFEKKHKSPSHESHGSDSASLSPSWFSTILNGRRIK 180

Query: 181 NQSSLSTAEEPVAAAERRH-CPVIERGMSPAGLSDSDEEH-EGPDRSPISQK---SPMAA 240
            QSSL+T EE +A AERRH C  I+RGMSPA +S+SDEE  E   RSPISQK   SPM  
Sbjct: 181 QQSSLATVEESIAGAERRHHCETIKRGMSPAIISESDEEECESHGRSPISQKFQQSPMTI 240

Query: 241 PGSAKRGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVTPPDITFSGNLRASPKPHLCAN 300
           PGSAKR + GHKQN SGFAFCLSPLVRASPN N NQKV  P+++FSGNLR   KPHLCAN
Sbjct: 241 PGSAKREKLGHKQNVSGFAFCLSPLVRASPNRNWNQKVMSPEVSFSGNLRVPGKPHLCAN 300

BLAST of MC05g0230 vs. ExPASy TrEMBL
Match: A0A5D3D3J1 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003230 PE=4 SV=1)

HSP 1 Score: 446 bits (1147), Expect = 1.27e-155
Identity = 234/310 (75.48%), Postives = 260/310 (83.87%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEK-QAELRSGAV---DDPLPPPLVF 60
           MRC+KH+SD +S++GVCASCLRERLL++IAAQAQAEK Q++L SG +   DDPLPPPL+F
Sbjct: 1   MRCKKHHSDFTSTVGVCASCLRERLLSIIAAQAQAEKNQSQLTSGGIRSADDPLPPPLLF 60

Query: 61  LRSVSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVTTGSF 120
           L SVSP+A   KS+ED+W++LDREGN R RHQRFYSTPQI PN RTNNSAN TFVTTGSF
Sbjct: 61  LHSVSPHA--TKSNEDLWTNLDREGNLRSRHQRFYSTPQIGPNCRTNNSANTTFVTTGSF 120

Query: 121 DQKPKSK-FSLWSKMFRSRSEKFEKNHKIPSRESRGPASYSSTPSWFSTIFAGRR-SNQS 180
           D+K +SK FSLWSK+FRSRSEKFEKNHK PSRES GP S SS+PSWFSTIF GRR   QS
Sbjct: 121 DRKQRSKKFSLWSKLFRSRSEKFEKNHKSPSRESHGPGSSSSSPSWFSTIFHGRRIKRQS 180

Query: 181 SLSTAEEPVAAAERRHCPVIERGMSPAGLSDSDEEHEGPDRSPISQK---SPMAAPGSAK 240
           SL+  EE +  AERRHC  IERGMSP  +SDSDEE EGPDRSPISQK   SPMAAPGSAK
Sbjct: 181 SLTPVEESIPVAERRHCQAIERGMSPVRVSDSDEECEGPDRSPISQKFQLSPMAAPGSAK 240

Query: 241 RGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVTPPDITFSGNLRASPKPHLCANRSRKI 300
           RGR GH QN SGFAFCLSPLVRASPN N NQKV PP+  FSGN++   KPHLCANRSRKI
Sbjct: 241 RGRLGHNQNVSGFAFCLSPLVRASPNRNWNQKVIPPETAFSGNIKVPAKPHLCANRSRKI 300

BLAST of MC05g0230 vs. ExPASy TrEMBL
Match: A0A1S3AVZ2 (uncharacterized protein LOC103483430 OS=Cucumis melo OX=3656 GN=LOC103483430 PE=4 SV=1)

HSP 1 Score: 446 bits (1147), Expect = 1.27e-155
Identity = 234/310 (75.48%), Postives = 260/310 (83.87%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEK-QAELRSGAV---DDPLPPPLVF 60
           MRC+KH+SD +S++GVCASCLRERLL++IAAQAQAEK Q++L SG +   DDPLPPPL+F
Sbjct: 1   MRCKKHHSDFTSTVGVCASCLRERLLSIIAAQAQAEKNQSQLTSGGIRSADDPLPPPLLF 60

Query: 61  LRSVSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVTTGSF 120
           L SVSP+A   KS+ED+W++LDREGN R RHQRFYSTPQI PN RTNNSAN TFVTTGSF
Sbjct: 61  LHSVSPHA--TKSNEDLWTNLDREGNLRSRHQRFYSTPQIGPNCRTNNSANTTFVTTGSF 120

Query: 121 DQKPKSK-FSLWSKMFRSRSEKFEKNHKIPSRESRGPASYSSTPSWFSTIFAGRR-SNQS 180
           D+K +SK FSLWSK+FRSRSEKFEKNHK PSRES GP S SS+PSWFSTIF GRR   QS
Sbjct: 121 DRKQRSKKFSLWSKLFRSRSEKFEKNHKSPSRESHGPGSSSSSPSWFSTIFHGRRIKRQS 180

Query: 181 SLSTAEEPVAAAERRHCPVIERGMSPAGLSDSDEEHEGPDRSPISQK---SPMAAPGSAK 240
           SL+  EE +  AERRHC  IERGMSP  +SDSDEE EGPDRSPISQK   SPMAAPGSAK
Sbjct: 181 SLTPVEESIPVAERRHCQAIERGMSPVRVSDSDEECEGPDRSPISQKFQLSPMAAPGSAK 240

Query: 241 RGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVTPPDITFSGNLRASPKPHLCANRSRKI 300
           RGR GH QN SGFAFCLSPLVRASPN N NQKV PP+  FSGN++   KPHLCANRSRKI
Sbjct: 241 RGRLGHNQNVSGFAFCLSPLVRASPNRNWNQKVIPPETAFSGNIKVPAKPHLCANRSRKI 300

BLAST of MC05g0230 vs. ExPASy TrEMBL
Match: A0A0A0L4I6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G128830 PE=4 SV=1)

HSP 1 Score: 441 bits (1135), Expect = 8.49e-154
Identity = 231/310 (74.52%), Postives = 259/310 (83.55%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEK-QAELRSGAV---DDPLPPPLVF 60
           MRC+KH+SD +S++GVCASCLRERLL++IAAQAQAEK Q++L  G +   DDPLPPPL+F
Sbjct: 1   MRCKKHHSDFTSTVGVCASCLRERLLSIIAAQAQAEKNQSQLTYGGIRSADDPLPPPLLF 60

Query: 61  LRSVSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVTTGSF 120
           + SVSP+A   KSDED+WS+LDREGNRR  HQRFYSTPQI PN RTNNSAN TFVTTGSF
Sbjct: 61  IHSVSPHA--TKSDEDLWSNLDREGNRRFLHQRFYSTPQIGPNGRTNNSANTTFVTTGSF 120

Query: 121 DQKPKSK-FSLWSKMFRSRSEKFEKNHKIPSRESRGPASYSSTPSWFSTIFAGRRSN-QS 180
           D+K +SK FSLWSK+FRSRS+KFEKNHK PSRES GP S SS+PSWFSTIF G R+  QS
Sbjct: 121 DRKQRSKKFSLWSKLFRSRSDKFEKNHKSPSRESHGPGSSSSSPSWFSTIFHGHRTKRQS 180

Query: 181 SLSTAEEPVAAAERRHCPVIERGMSPAGLSDSDEEHEGPDRSPISQK---SPMAAPGSAK 240
           SLS  EE ++ AERRHC  IERGMSP  +SDSDEE EGPDRSPISQK   SPMAAPGSAK
Sbjct: 181 SLSPVEESISVAERRHCHAIERGMSPVRVSDSDEECEGPDRSPISQKFQLSPMAAPGSAK 240

Query: 241 RGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVTPPDITFSGNLRASPKPHLCANRSRKI 300
           RGR GH QN SGFAFCLSPL+RASPN N NQK  PP+  FSGN++   KPHLCANRSRKI
Sbjct: 241 RGRLGHNQNVSGFAFCLSPLMRASPNRNWNQKAIPPETAFSGNIKVPAKPHLCANRSRKI 300

BLAST of MC05g0230 vs. ExPASy TrEMBL
Match: A0A6J1IG75 (uncharacterized protein LOC111473474 OS=Cucurbita maxima OX=3661 GN=LOC111474358 PE=4 SV=1)

HSP 1 Score: 421 bits (1082), Expect = 1.26e-145
Identity = 228/315 (72.38%), Postives = 251/315 (79.68%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEKQAELRSGAVDD-------PLPPP 60
           MRC+KH  DCSSSIGVCASCLRERL  +IAAQAQAEKQ+E+R+ A  D       PLPPP
Sbjct: 1   MRCKKHQFDCSSSIGVCASCLRERLFLIIAAQAQAEKQSEIRAAAAGDNRAADDRPLPPP 60

Query: 61  -LVFLRSVSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVT 120
            LVFLRSVSPY  R KSDE + SDLDREGNRR RHQRFYSTPQI P++RTNN+AN TFVT
Sbjct: 61  PLVFLRSVSPYDHRLKSDEGLLSDLDREGNRRNRHQRFYSTPQIGPDYRTNNTANSTFVT 120

Query: 121 TGSFDQKPKSKFSLWSKMFRSRSEKFEKNHKIPSRESRGPASYSSTPSWFSTIFAGRR-S 180
           TGSFD+K +SKFSLWSK+F+SRSEKFEK HK PS ES G  S S +PSWFSTI  GRR  
Sbjct: 121 TGSFDRKKRSKFSLWSKLFKSRSEKFEKKHKSPSHESHGSDSASLSPSWFSTILNGRRIK 180

Query: 181 NQSSLSTAEEPVAAAERRH-CPVIERGMSPAGLSDSDEEH-EGPDRSPISQK---SPMAA 240
            QSSL+T EE +A AERRH C  I+RGMSPA +S+SDEE  E   RSPISQK   SPM  
Sbjct: 181 QQSSLATVEESIAGAERRHHCETIKRGMSPAIISESDEEECESHGRSPISQKFQQSPMTI 240

Query: 241 PGSAKRGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVTPPDITFSGNLRASPKPHLCAN 300
           PGSAKR + GHKQN SGFAFCLSPLVRASPN N NQKV  P+++FSGNLR   KPHLCAN
Sbjct: 241 PGSAKREKLGHKQNVSGFAFCLSPLVRASPNRNWNQKVMSPEVSFSGNLRVPGKPHLCAN 300

BLAST of MC05g0230 vs. ExPASy TrEMBL
Match: A0A6J1FWR4 (uncharacterized protein LOC111447641 OS=Cucurbita moschata OX=3662 GN=LOC111447641 PE=4 SV=1)

HSP 1 Score: 414 bits (1064), Expect = 6.19e-143
Identity = 226/315 (71.75%), Postives = 250/315 (79.37%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEKQAELRSGAVDD-------PLPPP 60
           MRC+KH  DCSSSIGVCASCLRERL  +IAAQAQAEKQ+E+R+ A  D       PLPPP
Sbjct: 1   MRCKKHQFDCSSSIGVCASCLRERLFLIIAAQAQAEKQSEIRAAAAGDNRAADDRPLPPP 60

Query: 61  -LVFLRSVSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVT 120
            LVFLRSVSP+  R KSDE + SDLDREGNR   HQRFYSTPQI P++RTNN+AN TFVT
Sbjct: 61  PLVFLRSVSPFDHRLKSDEGLLSDLDREGNR---HQRFYSTPQIGPDYRTNNTANSTFVT 120

Query: 121 TGSFDQKPKSKFSLWSKMFRSRSEKFEKNHKIPSRESRGPASYSSTPSWFSTIFAGRR-S 180
           TGSFD+K +SKFSLWSK+F+SRSEKFEK HK PS ES G  S S +PSWFSTI  GRR  
Sbjct: 121 TGSFDRKKRSKFSLWSKLFKSRSEKFEKKHKSPSHESHGSDSASLSPSWFSTILNGRRIK 180

Query: 181 NQSSLSTAEEPVAAAERRH-CPVIERGMSPAGLSDSDEEH-EGPDRSPISQK---SPMAA 240
            QSSL+T EE +A AERRH C  I+RGMSPA +S+SDEE  E   RSPISQK   SPM  
Sbjct: 181 QQSSLATVEESIAGAERRHHCQTIKRGMSPAIISESDEEECESHGRSPISQKFQQSPMTI 240

Query: 241 PGSAKRGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVTPPDITFSGNLRASPKPHLCAN 300
           PGSAKR + GHKQN SGFAFCLSPLVRASPN N NQKV PP+++FSGNLR   KPHLCAN
Sbjct: 241 PGSAKREKLGHKQNVSGFAFCLSPLVRASPNRNWNQKVIPPEVSFSGNLRVPGKPHLCAN 300

BLAST of MC05g0230 vs. TAIR 10
Match: AT2G44600.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G60200.1); Has 56 Blast hits to 55 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 2; Fungi - 0; Plants - 52; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 174.1 bits (440), Expect = 1.7e-43
Identity = 124/324 (38.27%), Postives = 173/324 (53.40%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQAQAEK-QAELRSGAVDDPLPPPLVFLRS 60
           MRC++H  D SSSIGVCASCLRERL TL  + A +E    + R   +    PPPL+F RS
Sbjct: 1   MRCKRHTVDFSSSIGVCASCLRERLFTLAVSTAASENDDNDHRHSRIS---PPPLLFPRS 60

Query: 61  VSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVTTGSFDQK 120
           VSPY    KSD    +      +    + RF++TPQ+      ++S+   F +  SF +K
Sbjct: 61  VSPYVAPRKSDAGTGTGAGGRDSVASSNNRFFATPQVVVQ---SSSSEKVFESDRSF-KK 120

Query: 121 PKSKFSLWSKMFRSRSEKFEKNHKI--PSRESRGPASYSSTPSWFSTIFAGRRSNQSSLS 180
            KS  S +S  FR+RS+ ++        S     P+S +++ SWFS + + R   QS+ +
Sbjct: 121 KKSGLSRFSSFFRTRSDDYDSRRDSCDASTVFSQPSSATTSRSWFSKVISVRSKKQSTTN 180

Query: 181 TAE-EPVAAAE------------RRHCPVIERGMSPAGLSDSDEEHEGPDRSPISQKSPM 240
           T   E + A+E            +R+C    RGMSPAG S +++E        + +   M
Sbjct: 181 TCYIEDLIASESDHHHHNQNRPRQRYC----RGMSPAGDSTTNDESVEESPGRLRRTPVM 240

Query: 241 AAPGSAKRGRFGHKQNASGFAFCLSPLVRASPNWNLNQKVT-PPDITFSGNLRASPKPHL 300
             PG  K    G  ++ SG AFCLSPLVRA PN + N K   PPD  +SG L++  KPHL
Sbjct: 241 GTPGRKKTATIGIGRSVSGMAFCLSPLVRAKPNCSSNWKAKFPPDFGYSGELKSPAKPHL 300

Query: 301 ------CANRSRKIADFGRVNHNR 302
                 C NRS+K+ D GRV+H R
Sbjct: 301 STAASFCGNRSKKLVDLGRVDHRR 313

BLAST of MC05g0230 vs. TAIR 10
Match: AT3G60200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G44600.1); Has 60 Blast hits to 60 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 8; Fungi - 0; Plants - 51; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 144.8 bits (364), Expect = 1.1e-34
Identity = 125/317 (39.43%), Postives = 163/317 (51.42%), Query Frame = 0

Query: 1   MRCRKHNSDCSSSIGVCASCLRERLLTLIAAQA-QAEKQAELRSGAVDDPLPPPLVFLRS 60
           MRC++H  D SSS GVCASCLRERLL+L A+ A  A  +      + ++  PP L+F RS
Sbjct: 1   MRCKRHTVDLSSSNGVCASCLRERLLSLAASAAVSAAVEDNQSKKSNNNNHPPLLIFPRS 60

Query: 61  VSPYAPRAKSDEDVWSDLDREGNRRGRHQRFYSTPQIAPNHRTNNSANITFVTTGSFDQK 120
           VSPY  R KSD           + R    RF +TPQI        S    F +  S   K
Sbjct: 61  VSPYVTRRKSDAGAGGGDPLVSSNR----RFITTPQI---DLVGYSCK-DFESNRSNKSK 120

Query: 121 PKSKFSLWSKMFRSRSEKFEKNHKIPSRE------SRGPASYSSTPSWFSTIFA-GRRSN 180
              K S +S +FR+RSE F+ N K  +        S   +S SS+ SW STI + GRR  
Sbjct: 121 QGKKVSRFSNLFRARSEDFDTNPKSNNPRFSSCDASEISSSSSSSRSWISTILSTGRRKK 180

Query: 181 QSSLSTAEEPVAAAERRHCPVIERGMSPAGLSDSDEEHEGPDRSPISQKSPMAAPGSAKR 240
           Q + +   E V AA RR   +  RGMSP  + D++ E        + +      PG  K 
Sbjct: 181 QPTTACYIEDVIAA-RRPQRIYCRGMSP--VRDTEPEQSAESIEELRRTPATKTPGRRKI 240

Query: 241 GRFGHKQNASGFAFCLSPLVRASPNWNLNQKVT-PPDITFSGNLRASP-KPHL------C 300
              G  ++ SG AFCLSPLVRASPN    +K+  P +   SG + A P KPH+      C
Sbjct: 241 A-MGIGKSMSGMAFCLSPLVRASPNCPFKRKMRFPSEFGNSGEVTAVPEKPHISAAASFC 300

Query: 301 ANRSRKIADFGRVNHNR 302
           ANRS+K+ D GRV+  R
Sbjct: 301 ANRSKKLVDLGRVDRRR 305