MC05g0227 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0227 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.4e-26
Identity = 82/174 (47.13%), Postives = 101/174 (58.05%), Query Frame = 0

Query: 13  SLSLAISFLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALASESADLFKLGAIS 72
           S SL I FL  A   D+V  TCK    R P NV  DFCV+SL A   S SADL  LG IS
Sbjct: 4   SFSLCIFFLISA---DIVQGTCKKVAQRSP-NVNYDFCVKSLGADPKSHSADLQGLGVIS 63

Query: 73  IQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELYSDAGETLRDAAAEYASK 132
             L  +  S  + +I R+LK  K  P     LK  L DC  LY+DA  ++++A A++ SK
Sbjct: 64  ANLAIQQGSKIQTFIGRILK-SKVDP----ALKKYLNDCVGLYADAKSSVQEAIADFKSK 123

Query: 133 RYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLTAITLCIVTM 187
            Y  ANVK+S+ +D   TCEDGFKE   G  SP+TK N D  QLTAI+L I  +
Sbjct: 124 DYASANVKMSAALDDSVTCEDGFKE-KKGIASPVTKENKDYVQLTAISLAITKL 167

BLAST of MC05g0227 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 9.8e-26
Identity = 81/176 (46.02%), Postives = 106/176 (60.23%), Query Frame = 0

Query: 11  FFSLSLAISFLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALASESADLFKLGA 70
           FF+L L +   A+ +A D+V  TCK    R P NV  DFCV+SL A   S +ADL  LG 
Sbjct: 11  FFNLLLLLQ--AVISA-DIVQGTCKKVAQRSP-NVNYDFCVKSLGADPKSHTADLQGLGV 70

Query: 71  ISIQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELYSDAGETLRDAAAEYA 130
           IS  L  ++ S  + +I R+LK  K  P     LK  L DC  LY+DA  ++++A A++ 
Sbjct: 71  ISANLAIQHGSKIQTFIGRILK-SKVDP----ALKKYLNDCVGLYADAKSSVQEAIADFK 130

Query: 131 SKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLTAITLCIVTM 187
           SK Y  ANVK+S+ +D   TCEDGFKE   G VSP+TK N D  QLTAI+L I  +
Sbjct: 131 SKDYASANVKMSAALDDSVTCEDGFKE-KKGIVSPVTKENKDYVQLTAISLAITKL 176

BLAST of MC05g0227 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 4.9e-09
Identity = 56/179 (31.28%), Postives = 83/179 (46.37%), Query Frame = 0

Query: 12  FSLSLAISFL---AIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALASESA-DLFK 71
           F +SL I  L     A A  ++  +CK A A+DP  +  DFCV SL     S++A  L  
Sbjct: 3   FLVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDP-QLSYDFCVNSLTQDPQSKAATTLES 62

Query: 72  LGAISIQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELYSDAGETLRDAAA 131
           L   S +      ++ +  + + LK+++        +   L+ C   Y+DA + L  A A
Sbjct: 63  LVLASTKTAAAKITNLKGIVAQDLKDQRYQD-----IVEDLKLCLGFYNDANDDLTTALA 122

Query: 132 EYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLTAITLCIVTM 187
              S+ Y  AN+ LS+ +D    CED FKE      SP+T  NS  F+   I L    M
Sbjct: 123 NIKSRDYQGANINLSAALDVPGNCEDDFKE--AKKTSPITNENSILFKTILIPLAFTNM 173

BLAST of MC05g0227 vs. NCBI nr
Match: XP_022924291.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 232 bits (592), Expect = 1.43e-74
Identity = 126/191 (65.97%), Postives = 153/191 (80.10%), Query Frame = 0

Query: 1   MGSTSTHRFSFFSLSLAIS-FLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALA 60
           M    THRF   + SL ++ +L    A+DVV RTC+I+ A+DP NV LDFC+RSLAAA A
Sbjct: 1   MSCNYTHRFIALASSLFVALYLTSTVANDVVARTCEISAAKDP-NVHLDFCLRSLAAAPA 60

Query: 61  SESADLFKLGAISIQLIRRNASDTRKYIERLLKEEKA--SPDDSSFLKPRLRDCRELYSD 120
           S++ADL++LGAISI+LI RNA+ TR+YIERLLK++K   +P   S+L+P L DCRELYSD
Sbjct: 61  SDTADLYELGAISIELIGRNATSTRRYIERLLKKKKKKKNPSPDSYLRPPLNDCRELYSD 120

Query: 121 AGETLRDAAAEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLT 180
              T+RDAAA+YASKRY E NVKLSS+MDAVTTCEDGFKE+DGG VSPLTKRN DAF+LT
Sbjct: 121 VRVTVRDAAADYASKRYEEVNVKLSSIMDAVTTCEDGFKEMDGG-VSPLTKRNDDAFELT 180

Query: 181 AITLCIVTMRP 188
           AI LCI+ MRP
Sbjct: 181 AIALCILNMRP 189

BLAST of MC05g0227 vs. NCBI nr
Match: XP_023528439.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 231 bits (590), Expect = 2.88e-74
Identity = 127/191 (66.49%), Postives = 153/191 (80.10%), Query Frame = 0

Query: 1   MGSTSTHRFSFFSLSLAIS-FLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALA 60
           M    THRF   + SL ++ +L    A+DVV RTC+I+ A+DP NV LDFC+RSLAAA A
Sbjct: 1   MSCNYTHRFIALASSLFVALYLNSTVANDVVARTCEISAAKDP-NVHLDFCLRSLAAAPA 60

Query: 61  SESADLFKLGAISIQLIRRNASDTRKYIERLLKE--EKASPDDSSFLKPRLRDCRELYSD 120
           S+ ADL++LGAISI+LI RNA+ TR+YIERLLK+  +K +P   S+++PRL DCRELYSD
Sbjct: 61  SDMADLYELGAISIELIGRNATSTRRYIERLLKKNKKKKNPSLDSYVRPRLSDCRELYSD 120

Query: 121 AGETLRDAAAEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLT 180
              T+RDAAA+YASKRY E NVKLSSVMDAVTTCEDGFKE+DGG VSPLTKRN DAF+LT
Sbjct: 121 VKATVRDAAADYASKRYEEVNVKLSSVMDAVTTCEDGFKEMDGG-VSPLTKRNDDAFELT 180

Query: 181 AITLCIVTMRP 188
           AI LCI+ MRP
Sbjct: 181 AIALCILNMRP 189

BLAST of MC05g0227 vs. NCBI nr
Match: KAG6582515.1 (hypothetical protein SDJN03_22517, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018899.1 hypothetical protein SDJN02_20772, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 230 bits (587), Expect = 8.24e-74
Identity = 126/191 (65.97%), Postives = 152/191 (79.58%), Query Frame = 0

Query: 1   MGSTSTHRFSFFSLSLAIS-FLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALA 60
           M    THRF   + SL ++ +L    A+DVV RTC+I+ A+DP NV LDFC+RSLAAA A
Sbjct: 1   MSCNYTHRFIALASSLFVALYLNSTVANDVVARTCEISAAKDP-NVHLDFCLRSLAAAPA 60

Query: 61  SESADLFKLGAISIQLIRRNASDTRKYIERLLKE--EKASPDDSSFLKPRLRDCRELYSD 120
           S++ADL++LGAISI+LI RNA+ TR+YIERLLK+  +K +P   S+L+P L DCRELYSD
Sbjct: 61  SDTADLYELGAISIELIGRNATSTRRYIERLLKKNKKKKNPSPDSYLRPPLNDCRELYSD 120

Query: 121 AGETLRDAAAEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLT 180
              T+RDAAA+YASKRY E NVKLSSVMDA TTCEDGFKE+DGG VSPLTKRN DAF+LT
Sbjct: 121 VRVTVRDAAADYASKRYEEVNVKLSSVMDAATTCEDGFKEMDGG-VSPLTKRNDDAFELT 180

Query: 181 AITLCIVTMRP 188
           AI LCI+ MRP
Sbjct: 181 AIALCILNMRP 189

BLAST of MC05g0227 vs. NCBI nr
Match: XP_022980431.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 225 bits (573), Expect = 1.04e-71
Identity = 126/184 (68.48%), Postives = 150/184 (81.52%), Query Frame = 0

Query: 7   HRFSFFSLSLAISFLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALASESADLF 66
           HR S F L +A+ +L   AA+DVV R C+I  A+DP NV LDFC+RSLAAA AS+ ADL+
Sbjct: 8   HRASIF-LFVAL-YLNSTAANDVVARNCEIFAAKDP-NVHLDFCLRSLAAAPASDMADLY 67

Query: 67  KLGAISIQLIRRNASDTRKYIERLLKE--EKASPDDSSFLKPRLRDCRELYSDAGETLRD 126
           +LGAISI+LI RNA+ TR+YIERLLK+  +K +P   S+++PRL DCRELYSD   T+RD
Sbjct: 68  ELGAISIELIGRNATSTRRYIERLLKKNKKKKNPSLDSYVRPRLSDCRELYSDVKATVRD 127

Query: 127 AAAEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLTAITLCIV 186
           AAA+YASKRY E NVKLSSVMDAVTTCEDGFKE+DGG VSPLTKRN DAF+LTAI LCI+
Sbjct: 128 AAADYASKRYEEVNVKLSSVMDAVTTCEDGFKEMDGG-VSPLTKRNDDAFELTAIALCIL 187

Query: 187 TMRP 188
            MRP
Sbjct: 188 NMRP 187

BLAST of MC05g0227 vs. NCBI nr
Match: XP_038905481.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 224 bits (570), Expect = 2.87e-71
Identity = 125/191 (65.45%), Postives = 155/191 (81.15%), Query Frame = 0

Query: 1   MGSTSTHRFSF--FSLSLAISFLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAAL 60
           M  T THRF    +S  LA SF +  A++D+VLRTC+I+ A DP NVRLDFC+RSLAA  
Sbjct: 1   MSFTYTHRFIALAYSFLLAFSFQS-TASNDIVLRTCEISAASDP-NVRLDFCLRSLAADP 60

Query: 61  ASESADLFKLGAISIQLIRRNASDTRKYIERLLKEEKA-SPDDSSFLKPRLRDCRELYSD 120
           AS++ADL++LGAISI+LI RNA+ TR+YI+RLLK++K  SPD  S+++PRL DC+ELYSD
Sbjct: 61  ASDTADLYELGAISIELIGRNATSTRRYIDRLLKKKKKLSPD--SYVRPRLSDCKELYSD 120

Query: 121 AGETLRDAAAEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLT 180
           A ET+ D++AEYA KRY E NVKLSSVMDAVTTCEDGFKE++GG +SPLTKRN D F+LT
Sbjct: 121 AVETVGDSSAEYARKRYEEVNVKLSSVMDAVTTCEDGFKEMEGG-ISPLTKRNGDVFELT 180

Query: 181 AITLCIVTMRP 188
           AI LCI+ M P
Sbjct: 181 AIALCILNMHP 186

BLAST of MC05g0227 vs. ExPASy TrEMBL
Match: A0A6J1E955 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111431821 PE=4 SV=1)

HSP 1 Score: 232 bits (592), Expect = 6.93e-75
Identity = 126/191 (65.97%), Postives = 153/191 (80.10%), Query Frame = 0

Query: 1   MGSTSTHRFSFFSLSLAIS-FLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALA 60
           M    THRF   + SL ++ +L    A+DVV RTC+I+ A+DP NV LDFC+RSLAAA A
Sbjct: 1   MSCNYTHRFIALASSLFVALYLTSTVANDVVARTCEISAAKDP-NVHLDFCLRSLAAAPA 60

Query: 61  SESADLFKLGAISIQLIRRNASDTRKYIERLLKEEKA--SPDDSSFLKPRLRDCRELYSD 120
           S++ADL++LGAISI+LI RNA+ TR+YIERLLK++K   +P   S+L+P L DCRELYSD
Sbjct: 61  SDTADLYELGAISIELIGRNATSTRRYIERLLKKKKKKKNPSPDSYLRPPLNDCRELYSD 120

Query: 121 AGETLRDAAAEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLT 180
              T+RDAAA+YASKRY E NVKLSS+MDAVTTCEDGFKE+DGG VSPLTKRN DAF+LT
Sbjct: 121 VRVTVRDAAADYASKRYEEVNVKLSSIMDAVTTCEDGFKEMDGG-VSPLTKRNDDAFELT 180

Query: 181 AITLCIVTMRP 188
           AI LCI+ MRP
Sbjct: 181 AIALCILNMRP 189

BLAST of MC05g0227 vs. ExPASy TrEMBL
Match: A0A6J1ITK8 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111479799 PE=4 SV=1)

HSP 1 Score: 225 bits (573), Expect = 5.02e-72
Identity = 126/184 (68.48%), Postives = 150/184 (81.52%), Query Frame = 0

Query: 7   HRFSFFSLSLAISFLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALASESADLF 66
           HR S F L +A+ +L   AA+DVV R C+I  A+DP NV LDFC+RSLAAA AS+ ADL+
Sbjct: 8   HRASIF-LFVAL-YLNSTAANDVVARNCEIFAAKDP-NVHLDFCLRSLAAAPASDMADLY 67

Query: 67  KLGAISIQLIRRNASDTRKYIERLLKE--EKASPDDSSFLKPRLRDCRELYSDAGETLRD 126
           +LGAISI+LI RNA+ TR+YIERLLK+  +K +P   S+++PRL DCRELYSD   T+RD
Sbjct: 68  ELGAISIELIGRNATSTRRYIERLLKKNKKKKNPSLDSYVRPRLSDCRELYSDVKATVRD 127

Query: 127 AAAEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLTAITLCIV 186
           AAA+YASKRY E NVKLSSVMDAVTTCEDGFKE+DGG VSPLTKRN DAF+LTAI LCI+
Sbjct: 128 AAADYASKRYEEVNVKLSSVMDAVTTCEDGFKEMDGG-VSPLTKRNDDAFELTAIALCIL 187

Query: 187 TMRP 188
            MRP
Sbjct: 188 NMRP 187

BLAST of MC05g0227 vs. ExPASy TrEMBL
Match: A0A1S3AVZ7 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103483434 PE=4 SV=1)

HSP 1 Score: 216 bits (551), Expect = 1.11e-68
Identity = 118/193 (61.14%), Postives = 147/193 (76.17%), Query Frame = 0

Query: 1   MGSTSTHRFSFFSLSLAISFLAIA-----AADDVVLRTCKIAVARDPDNVRLDFCVRSLA 60
           M  T THR+    ++LA SF+ +      +A+D+V RTC+ + ARDP NVRLDFC+RSLA
Sbjct: 1   MSFTYTHRY----IALAFSFVVLLNFEPISANDIVSRTCETSAARDP-NVRLDFCLRSLA 60

Query: 61  AALASESADLFKLGAISIQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELY 120
           AA  S++ADL++LGAISI+LI RNA+ T++YIERLLK EK      S+++PRL DC ELY
Sbjct: 61  AAPGSDTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELY 120

Query: 121 SDAGETLRDAAAEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQ 180
           SDA ET+ +AAAEY  KRY E NVKLSSVMDAVTTCEDGFKE++   VSPLTKRN D F+
Sbjct: 121 SDAVETVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESR-VSPLTKRNGDVFE 180

Query: 181 LTAITLCIVTMRP 188
           L AI LCI+ +RP
Sbjct: 181 LAAIALCILDLRP 187

BLAST of MC05g0227 vs. ExPASy TrEMBL
Match: A0A5A7U1K7 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003190 PE=4 SV=1)

HSP 1 Score: 215 bits (548), Expect = 3.17e-68
Identity = 118/193 (61.14%), Postives = 146/193 (75.65%), Query Frame = 0

Query: 1   MGSTSTHRFSFFSLSLAISFLAIA-----AADDVVLRTCKIAVARDPDNVRLDFCVRSLA 60
           M  T THR     ++LA SF+ +      +A+D+V RTC+ + ARDP NVRLDFC+RSLA
Sbjct: 1   MSFTYTHR----CIALAFSFVVLLNFEPISANDIVSRTCETSAARDP-NVRLDFCLRSLA 60

Query: 61  AALASESADLFKLGAISIQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELY 120
           AA  S++ADL++LGAISI+LI RNA+ T++YIERLLK EK      S+++PRL DC ELY
Sbjct: 61  AAPGSDTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELY 120

Query: 121 SDAGETLRDAAAEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQ 180
           SDA ET+ +AAAEY  KRY E NVKLSSVMDAVTTCEDGFKE++   VSPLTKRN D F+
Sbjct: 121 SDAVETVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESR-VSPLTKRNGDVFE 180

Query: 181 LTAITLCIVTMRP 188
           L AI LCI+ +RP
Sbjct: 181 LAAIALCILDLRP 187

BLAST of MC05g0227 vs. ExPASy TrEMBL
Match: A0A0A0L7T4 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G128870 PE=4 SV=1)

HSP 1 Score: 200 bits (509), Expect = 2.49e-62
Identity = 112/189 (59.26%), Postives = 140/189 (74.07%), Query Frame = 0

Query: 1   MGSTSTHRFSFFSLSLAISF-LAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALA 60
           M  T  HRF   +LS  + F L   +A+D + R C+++ A DP NVRLDFC++SLAAA  
Sbjct: 1   MSITYMHRFIALALSFFVLFNLRSISANDTLSRACELSAASDP-NVRLDFCLQSLAAAPG 60

Query: 61  SESADLFKLGAISIQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELYSDAG 120
           S++ADL++LGA+SI+LI  NA+ TR+YIERLLK EK SPD   +++PRL DC ELY DA 
Sbjct: 61  SDTADLYELGALSIKLIAWNATSTRRYIERLLKNEKKSPD--PYVRPRLSDCEELYIDAI 120

Query: 121 ETLRDAAAEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLTAI 180
           + + DAA EY   RY E NVKLSSVMDAVTTCEDGFKE++G  VSPLTKRN D F+LTAI
Sbjct: 121 KAVGDAAFEYGRNRYEEVNVKLSSVMDAVTTCEDGFKEMEGR-VSPLTKRNGDVFELTAI 180

Query: 181 TLCIVTMRP 188
            L I+ +RP
Sbjct: 181 ALSILNLRP 185

BLAST of MC05g0227 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 75.5 bits (184), Expect = 5.1e-14
Identity = 63/189 (33.33%), Postives = 94/189 (49.74%), Query Frame = 0

Query: 11  FFSLSLAISFLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSL---AAALASESADLFK 70
           F+ + L      + +  D++ +TCK   A+       +FCV SL     AL S + +L  
Sbjct: 7   FYIIFLFFLVSQVKSTSDMIDQTCKSCAAKS-TIFDYNFCVSSLNNSPIALPSPT-NLSS 66

Query: 71  LGAISIQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELYSDAGETLRDAAA 130
           L  + +     NA+ T   I++LL     S DD SF    LRDC ELY DA + L +A  
Sbjct: 67  LALVPMLQALDNATATASTIQQLL----ISDDDGSFRSACLRDCLELYEDATDRLEEAVR 126

Query: 131 EYASKRYV-EANVKLSSVMDAVTTCEDGFKEID-----GGGV----SPLTKRNSDAFQLT 187
            + +++ +   NV +S+ M++  TCE+GF+E D     GGGV    SP+   N   F+  
Sbjct: 127 VFITRKELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFG 186

BLAST of MC05g0227 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 8.2e-12
Identity = 54/179 (30.17%), Postives = 90/179 (50.28%), Query Frame = 0

Query: 12  FSLSLAISFLAI---AAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALASESA-DLFK 71
           FSL L + FL +   A A  ++  +CK A  +DP  ++ DFCV+SL     S++A  L  
Sbjct: 3   FSLYLVMFFLLLNCFATAQSLIRDSCKKATTKDP-KLKYDFCVKSLEENPQSKTAKSLEG 62

Query: 72  LGAISIQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELYSDAGETLRDAAA 131
           L  +S +      +  +  ++++LKE+K        ++  L DC ELY++A ++L  +  
Sbjct: 63  LVFVSTKNAVSKTTSLKGMVDKILKEDKYE------VERPLLDCLELYTEAIDSLNQSLD 122

Query: 132 EYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLTAITLCIVTM 187
              S+ Y  A + +S+ MDA  +CE  F +      SP TK N   F +  I +   +M
Sbjct: 123 TVKSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

BLAST of MC05g0227 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 8.2e-12
Identity = 53/167 (31.74%), Postives = 83/167 (49.70%), Query Frame = 0

Query: 9   FSFFSLSLAISFLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALASESA-DLFK 68
           F F+     +   A   A  ++   CK A  ++P  +  +FCV+SL     S++A  L +
Sbjct: 3   FLFYFAMFFLLLNAFTIAQSLIQDFCKKAADKNP-KIHYNFCVKSLEENPQSKTARSLDR 62

Query: 69  LGAISIQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELYSDAGETLRDAAA 128
           L  +S +      +  +  ++++LKE +       + +  LRDC ELYSDA  +L++A  
Sbjct: 63  LVMLSTKNAVSKTTSMKGIVDKILKENRF----EMYSEKPLRDCLELYSDATNSLKEALT 122

Query: 129 EYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAF 175
              S+ Y  ANV +S+ M A  +CE GFKE      SP TK N   F
Sbjct: 123 IIKSRDYKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

BLAST of MC05g0227 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.1e-11
Identity = 52/180 (28.89%), Postives = 87/180 (48.33%), Query Frame = 0

Query: 8   RFSFFSLSLAISFLAIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALASESA-DLF 67
           +F  + ++  +    +A    ++  +CK A A  P   + + CV SL     +++A DL 
Sbjct: 2   KFLLYLVTFFVLSNGLANGQTLIRNSCKKATATSP-KFKYNLCVTSLETNPQAKTAKDLA 61

Query: 68  KLGAISIQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELYSDAGETLRDAA 127
            L   S +     A+  +  +++++K +K     +      LRDC +LY+DA  +L +A 
Sbjct: 62  GLVMASTKNAVTKATTLKGTVDKIIKGKKV----NKMTAMPLRDCLQLYTDAIGSLNEAL 121

Query: 128 AEYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLTAITLCIVTM 187
           A   S+ Y      LS+ MD  +TCE GFKE      SP+TK N + +Q+  I L    M
Sbjct: 122 AGVKSRNYPTVKTVLSAAMDTPSTCETGFKE--RKAPSPVTKENDNLYQMILIPLAFTNM 174

BLAST of MC05g0227 vs. TAIR 10
Match: AT5G46950.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.6e-10
Identity = 55/179 (30.73%), Postives = 88/179 (49.16%), Query Frame = 0

Query: 12  FSLSLAISFL---AIAAADDVVLRTCKIAVARDPDNVRLDFCVRSLAAALASESA-DLFK 71
           F +SL I  L     A A  ++  +CK A A+DP +   DFCV+SL     S++A  L  
Sbjct: 3   FLVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQS-SYDFCVQSLTQDPQSKAATTLED 62

Query: 72  LGAISIQLIRRNASDTRKYIERLLKEEKASPDDSSFLKPRLRDCRELYSDAGETLRDAAA 131
           L   S + +    ++ +  + + LK+++        +   L+ C   Y DA ++L+ A A
Sbjct: 63  LALASTKNVAAKITNLKGIVAQDLKDQRYQD-----IVEDLKLCLGFYKDANDSLKTALA 122

Query: 132 EYASKRYVEANVKLSSVMDAVTTCEDGFKEIDGGGVSPLTKRNSDAFQLTAITLCIVTM 187
              S+ Y  AN  LS+ ++A   CED FKE +    SP+T  N+  ++   I L   TM
Sbjct: 123 NIKSRDYDGANSNLSAALNAPGDCEDDFKEAE--KKSPITNENNILYKTIVIPLAFTTM 173