MC05g0186 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0186 vs. ExPASy Swiss-Prot
Match: Q8W4N3 (Ubiquitin carboxyl-terminal hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=UBP2 PE=1 SV=2)

HSP 1 Score: 653.3 bits (1684), Expect = 4.4e-186
Identity = 438/1057 (41.44%), Postives = 579/1057 (54.78%), Query Frame = 0

Query: 1   MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
           MGKK KKK  RAP KE +   IS     +  S   EIA  D   VKE + C H DK LNL
Sbjct: 1   MGKKAKKK-ARAPTKEIQTMEISKKVSEEPPSQAGEIAEGDVKAVKETQACVHFDKALNL 60

Query: 61  SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
            ++  KI SS  I C +C E    +RG+K K   GKK   +  D KS++KAIW+CL+CG 
Sbjct: 61  EKVLDKIKSSRQIKCAECNEGVYGKRGTKAKGSKGKKDFSSS-DPKSNNKAIWLCLECGC 120

Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
           Y CGG+GLP   QSH +RH R TRH +VIQ+ENPQLRWCF C  L+PVEK E+N ++KD 
Sbjct: 121 YVCGGVGLPNGPQSHVLRHSRVTRHRLVIQWENPQLRWCFPCQLLLPVEK-EDNGEKKDV 180

Query: 181 LSNVVKLIRDRSMEG-SPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLG 240
           LS VVKLI+ RS+   +  D+E+    S  +T+  K E ++ SD++ R+ YVV+GL+NLG
Sbjct: 181 LSEVVKLIKGRSLNNLASSDIEDQCSGSGSITSDIKLEGAVTSDIEARDGYVVRGLVNLG 240

Query: 241 NTCFFNSILQNLLAIDMLRDYFLKL--EECVGPLTIALKKLFIEVRMEGKIKGSINPRSV 300
           NTCFFNSI+QNLL++D LRD+FLK       GPL  +L+KLF E + E  +K  INPR+ 
Sbjct: 241 NTCFFNSIMQNLLSLDRLRDHFLKENGSGVGGPLASSLRKLFTETKPEAGLKSVINPRAF 300

Query: 301 FGCISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKM--TKSKEEKTSGNSTPTFVD 360
           FG   SKAPQF+GYDQHDSHELLR LLD LSTEE A RK       +EK++     T ++
Sbjct: 301 FGSFCSKAPQFRGYDQHDSHELLRCLLDSLSTEESALRKKRGVSDNDEKST-----TLIE 360

Query: 361 EMFGGQISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSG 420
            +FGG+ SS V C+ECGH+S VYEPFLDLSLPVP KK   KK QPVSRAKK K+PPKR  
Sbjct: 361 SVFGGETSSIVSCMECGHSSKVYEPFLDLSLPVPFKKSPPKKPQPVSRAKKAKLPPKRVP 420

Query: 421 KTFPKTEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKE 480
           K   K  K S V+   + S  +SS +S      S    S     +L+  +   V E+   
Sbjct: 421 KNVSKVSKVSKVLPGMVLSELNSSGKSMAVTADSDTSCS-----SLAPLDNGPVLETPSV 480

Query: 481 VLIEN--GGECTSDSLT-----WLDFLGPEPNAENCNTSTTQDS---------------- 540
           + ++N    E  S S T     WLDF+GPE + +  N    +D                 
Sbjct: 481 LTLDNNQASESASQSDTGFDGSWLDFIGPETSGDETNLDMQEDGIDNVITAEVNQIVPSP 540

Query: 541 ---------------ENNVEVFIND------------------------DSQQSISGSSN 600
                          E N E  + D                         +   IS   +
Sbjct: 541 NIVANSSVSSGDQTLEGNTERLMQDYEEIAKAEANLDEKDVQAMQSDECPATSGISAEFS 600

Query: 601 QGSSLHNEPSQQPDFSSINLWDDES-PLQVQASEVLLLPYKEESSTTEVAKEDDQASSSI 660
           Q S +  +P      SS+N WD+E  PL V  S++L +PYKE S   +  + + +ASSS 
Sbjct: 601 QASCIGCDPGIGESSSSVNPWDEEELPLVVADSQILYMPYKEISCNDKSVEGECEASSSF 660

Query: 661 LGCAQD--DLDGFGLGDMFDEPEITVGPL---PRPSTVNEVSESSFISESDPDEVDNSDS 720
           +    +  + D    G +FDEPE T GP+   P  +  + V   +F SESDP+E+D+SD 
Sbjct: 661 VTGDHEPQNSDFVDFGGLFDEPETTEGPVFGPPSKAEASGVGFMAFSSESDPEEIDDSDL 720

Query: 721 PVSVESCLTYFTKPELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGG 780
           PVSVE CL +FTK E+LS++N +NCE C K L+ QRL  K++S                 
Sbjct: 721 PVSVERCLGHFTKHEILSDDNAWNCENCSKNLKLQRLREKRKS----------------- 780

Query: 781 DISCLNENSSVEVKNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNS 840
                NE+ S      R+ +  NG                                    
Sbjct: 781 -----NEDES------RSSNTSNG------------------------------------ 840

Query: 841 LVLDENEAKVDGDVNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLS 900
             + ENE +  G+              + +++ +D S     ++D R +           
Sbjct: 841 -WVKENEDEGFGETEI----------LAVKQDPNDTSCVKDHSSDGRKAARIH------- 900

Query: 901 SQISVENSELEKSEDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYS 960
              S + SE + ++D + DS+  ++ VKRDATK+ LI+KAPP+LTIH+KRFS D RGR S
Sbjct: 901 ---SADESESKGTQDEDEDSEK-VITVKRDATKKVLINKAPPVLTIHLKRFSQDLRGRLS 955

Query: 961 KLNGHVRFKETIDLKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSS 985
           KLNGHV FKE IDL+ YMD+RC   D   YRL G+VEHSG+MRGGHYVAYVRGG R +  
Sbjct: 961 KLNGHVAFKEVIDLRQYMDSRCSGEDPPVYRLAGLVEHSGTMRGGHYVAYVRGGQRVK-- 955

BLAST of MC05g0186 vs. ExPASy Swiss-Prot
Match: Q9FPT5 (Ubiquitin carboxyl-terminal hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=UBP1 PE=1 SV=2)

HSP 1 Score: 490.7 bits (1262), Expect = 3.8e-137
Identity = 398/1145 (34.76%), Postives = 552/1145 (48.21%), Query Frame = 0

Query: 29   QQSSTTVEIAGDDASVVKEKRECRHLDKCLNLSELSSKIGSSEPINCEDCQESSADRRGS 88
            +QS  +VE+  + A  + EKR C H DK ++L +L   I S + I C +C E    +RGS
Sbjct: 10   KQSEQSVEVTEEFAVTMTEKRSCVHFDKYVDLDKLLKIIKSYQQIKCGECNERVHVKRGS 69

Query: 89   KGKAKHGKKKGGAPVDVKSDSKAIWICLQCGHYACGGIGLPTNSQSHAVRHVRQTRHPVV 148
            +  A + ++   +  D      AIW+CL+CG+Y CG +GLPT +QSH + H R TRH +V
Sbjct: 70   RWGASN-RRDWYSSSDQNCARNAIWLCLECGYYVCGDVGLPTEAQSHVMGHNRLTRHRLV 129

Query: 149  IQFENPQLRWCFSCNTLIPVEKTEENADQKDGLSNVVKLIRDRSMEGSPVDVENTWRASS 208
            IQ +NPQLRWCFSC +L+P +  EEN ++KD L  VVKLIR+RS         NT+ AS 
Sbjct: 130  IQCKNPQLRWCFSCQSLLPFD-NEENGEKKDLLLEVVKLIRERS--------PNTYSASF 189

Query: 209  EVTAATKSESSILSDLDRRNHYVVKGLINLGNTCFFNSILQNLLAIDMLRDYFLKLEECV 268
            E   +     SI   ++ R+ Y V+GL+NLGNTCFFNS++QNLL++D LR++FLK +  V
Sbjct: 190  ETEFS--CSGSIYGGIEARDGYAVRGLVNLGNTCFFNSVMQNLLSLDQLREHFLKEDLSV 249

Query: 269  -GPLTIALKKLFIEVRMEGKI-KGSINPRSVFGCISSKAPQFKGYDQHDSHELLRVLLDG 328
             GPL  +LK+LF E   E  + +  INPR +F  + S+APQF+GY QHDSHELLR LLDG
Sbjct: 250  SGPLVSSLKELFAESNSEASVFRNEINPRDLFFSVCSQAPQFRGYQQHDSHELLRCLLDG 309

Query: 329  LSTEELASR-KMTKSKEEKTSGNSTPTFVDEMFGGQISSAVCCIECGHTSTVYEPFLDLS 388
            LS EE + R K+  S    +S    PT +D +FGG+ISS V C+ECGH S VYEPF+DLS
Sbjct: 310  LSIEESSLRKKLGVSDSNDSSTYQKPTLIDSVFGGEISSTVSCLECGHFSKVYEPFMDLS 369

Query: 389  LPVPIKKPLAKKVQPVSRAKK-----------------------TKVP------------ 448
            LPVP  K   KK Q +S+AK+                       + VP            
Sbjct: 370  LPVPSMKLPPKKQQILSQAKEVLKNGAVSKDSEVVSAKPASDHNSTVPLFPSDHKIQSRP 429

Query: 449  -----------------------------------------PKRSGKTFPKTEKASDVVS 508
                                                       R+GKT P  E   DV +
Sbjct: 430  ETSDNETDLVLLSDVSDTAPSTEAKGVNQILVGSTETLMHDNDRTGKTVPDKE---DVRA 489

Query: 509  VQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSD----VKESEKEVL-------- 568
             Q     S+S  S++  EA   G   + + + S+   +D    +  ++ +VL        
Sbjct: 490  TQSNEETSASGISAVIDEAQVCGCPDLEQSSSSANQGADEELALMVADSQVLYMPYKDHL 549

Query: 569  --------------IENGGECTSDSLTWLDFLGPEPNAENCNT----STTQDSENNVEVF 628
                          +    E   D   +  FL  EP           S ++  E   +  
Sbjct: 550  FYDDYMVAEASSSFVSGDHEPKKDYFDFSSFL-DEPEIREGPVFRPLSKSEVYEAGFKAD 609

Query: 629  INDDSQQSISGSSNQGSSLHNEPSQQPDFSSINLWDDESPLQ-------VQASEVLLLPY 688
             +DD   S        S + ++  Q  D+   + + DE  +        +  SEV    +
Sbjct: 610  CSDDKTVSAGKGEASSSFISSDHEQNIDYVDFSSFFDEPEISERPFFRPLSKSEVSEAGF 669

Query: 689  KEESS--TTEVAKEDDQASSSILGCAQDDLDGFGLGDMFDEPEITVGPLPRPSTVNEVSE 748
            K + S   T  A + + +SS +    + ++D       FDEPEI+  P  RP + +EVSE
Sbjct: 670  KADCSDDKTVSAGKGEASSSFVSSDHEQNIDYVDFSSFFDEPEISERPFFRPLSKSEVSE 729

Query: 749  SSFI-------------------------------------------------------- 808
            + F+                                                        
Sbjct: 730  AGFMAVSGNDKTVRAGKGETFSSFMSGDNERNIDYVEFTNRIFDDRGTSERPVFGPPSKA 789

Query: 809  -----------SESDPDEVDNSDSPVSVESCLTYFTKPELLSNENGYNCEKCLKRLQQQR 868
                       S+SDP  +D SDSPVSV+ CL  FTK E+LS +N ++CE C K L+ QR
Sbjct: 790  KVSEAGFVAVSSDSDPAVLDESDSPVSVDRCLAQFTKHEILSEDNAWHCENCSKNLKLQR 849

Query: 869  LEMKKQSKVA---REVVVNGCQTAVGGDISCLNENSSVEVKNQRNVSLVNGSMCYSSGES 928
            L  K+++K     R V  NG  +A      C   +SS+   NQ  + L NG   Y +   
Sbjct: 850  LREKRRTKEGLSNRWVNENGASSAFD---EC--RDSSL---NQSCIDLENG---YKAAPP 909

Query: 929  MNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGDVNSGQAYSSGCSNTSNRENS 985
            +             TK  +C+         +E  A  DG V                EN+
Sbjct: 910  I-------------TKLPNCK---------EEESAIDDGFV--------------GEENT 969

BLAST of MC05g0186 vs. ExPASy Swiss-Prot
Match: Q6PAW2 (Ubiquitin carboxyl-terminal hydrolase 16 OS=Xenopus laevis OX=8355 GN=usp16 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 1.4e-46
Identity = 255/1013 (25.17%), Postives = 392/1013 (38.70%), Query Frame = 0

Query: 51  CRHLDKCLNLSELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSK 110
           C+HL K L+   +   + + E   C+DCQ  + ++  S+              D  ++  
Sbjct: 21  CKHLRKALDDGSVKRALVNVEWTMCQDCQADNKEKNNSE--------------DESAEDP 80

Query: 111 AIWICLQCGHYACGGIGLPTNSQSHAVRHVRQTR---HPVVIQFENPQLRWCFSCNTLIP 170
           ++W+CL+CGH  CG      +   HA+ H    R   H +V+  +     WC+ C+  +P
Sbjct: 81  SVWLCLKCGHRGCG----RNSVAQHALNHYNTPRSEPHCLVLNVD-MWSAWCYLCDNEVP 140

Query: 171 VEKT----------EENADQKDGLSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSE 230
             +T          +  A  K+  SN V    +   E    +     +   E       +
Sbjct: 141 YNRTSRLGQLVDYLQRKAKAKNKNSNSVVSNEEVKTEIVTENEVKKIQYQDEKPDVQAKQ 200

Query: 231 SSILSDLDRRNHYVVKGLINLGNTCFFNSILQNLLAIDMLRDYF---------------- 290
               S         VKGL NLGNTCFFN+++QNL     L +                  
Sbjct: 201 EKASSTQKISTEPTVKGLSNLGNTCFFNAVMQNLSQTPALSELLNEVKTFRKPVTVLLPD 260

Query: 291 --------LKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGCISSKAPQFKGYDQ 350
                   + LE+  GPLT+A+ +   E  M    KG + P+ +F  +  KA +FKGY Q
Sbjct: 261 SSSPKILEVNLEQQPGPLTLAMWQFLTE--MHETKKGVVTPKELFSQVCKKAIRFKGYQQ 320

Query: 351 HDSHELLRVLLDGLSTEELASRKMTKSK---------------EEKTSGNSTPTFVDEMF 410
            DS ELLR LLDG+  EE+    +  SK               ++     + P FVD +F
Sbjct: 321 QDSQELLRYLLDGMRGEEIQRVTLAMSKSLQSTLDEEEIKKIVKDYEKRRTIPNFVDCLF 380

Query: 411 GGQISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTF 470
           GG+++S + C EC   S V+EPFLDLSLPV +   + KK      +K T  PP R  K  
Sbjct: 381 GGELTSTIMCEECHTVSLVHEPFLDLSLPV-LDDLIVKK-----NSKST--PPARERKEE 440

Query: 471 PKTEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLI 530
            + E+  D   V+     S  +   L  +A          K  +       K   K VL 
Sbjct: 441 EEEEENDDDRYVKERDEVSPGASKHLQKKAKKAA------KKQAKNQRRQQKWQGKTVLF 500

Query: 531 EN--GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGS 590
            +    EC+ D          E       T+T  D+E      +N               
Sbjct: 501 TDLAKQECSED----------EEEVSQTKTNTRPDNETPTADGLN--------------- 560

Query: 591 SLHNEPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCA 650
                 + + D S++   ++ES   +   E        + ST E   E+           
Sbjct: 561 ------TMETDLSTLENGNEESADGLNTMET-------DLSTLENGNEEMAG-------- 620

Query: 651 QDDLDGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLT 710
                GF   +M D   +  G     S V  ++E + +  S  + V + ++   V +   
Sbjct: 621 -----GFKTMEM-DLSTLENGSETIKSAVEGITEHTDLDSSVHNNVGSVETNALVGN--- 680

Query: 711 YFTKPELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGD---ISCLN 770
                  + N N     K  +R                           GGD        
Sbjct: 681 -------MENNNNIEVNKTPER-----------------------TAGSGGDSMEAMAAV 740

Query: 771 ENSSVEVKNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDEN 830
           EN + +  N  +   VNG +     +S N+   +  S       +D +  ++   +L + 
Sbjct: 741 ENGNADAVNVDDTEAVNGLI-----DSANMDHELTNSLNRLQLSSDLEPTQVEIEILPDK 800

Query: 831 E---AKVDGDVNSGQAYSSGCSNTSNREN--SDDRSSYSLSNNDSRGSGLAKSNIEPLSS 890
           E    +V   VN      +  S  SNR++   D+ S  S     +    L  +N    + 
Sbjct: 801 EQPHTQVYEVVNEDP--KTAFSTLSNRKDLPIDEFSVLSCLYQFTHKETLTGNNKLLCNV 860

Query: 891 QISVENSELEKSEDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSK 950
               + S L  S  GE         V  +A K+ L+    PILT+H+KRF  +      K
Sbjct: 861 CTRKQASRLNNSNKGEKKF------VYTNAKKQMLVSNPSPILTLHLKRFQQNG-FNLRK 898

Query: 951 LNGHVRFKETIDLKPYMDTRC----EDRDKCSYRLIGVVEHSGSMRGGHYVAYVR----- 985
           +N H++F E +DL P+   +C    E   +  Y L GV+EHSGSMR GHY A+V+     
Sbjct: 921 INRHIKFPEVLDLAPFCTAKCKNVPEGESRLLYSLYGVIEHSGSMRSGHYTAFVKLRHPN 898

BLAST of MC05g0186 vs. ExPASy Swiss-Prot
Match: Q4R6X7 (Ubiquitin carboxyl-terminal hydrolase 16 OS=Macaca fascicularis OX=9541 GN=USP16 PE=2 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 3.1e-46
Identity = 246/1026 (23.98%), Postives = 389/1026 (37.91%), Query Frame = 0

Query: 40  DDASVVKEKRECRHLDKCLNLSELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKG 99
           DD+S   E   CRH+ K L    L   + + E   C+DC+  +      K KA+      
Sbjct: 14  DDSSETLEPM-CRHIRKGLEQGNLKKALVNVEWNICQDCKTDNK----VKDKAE------ 73

Query: 100 GAPVDVKSDSKAIWICLQCGHYACGGIGLPTNSQSHAVRHV---RQTRHPVVIQFENPQL 159
               +   +  ++W+CL+CGH  CG      + + HA++H    R   H +V+  +N  +
Sbjct: 74  ----EETEEKPSVWLCLKCGHQGCG----RNSQEQHALKHYLTPRSEPHCLVLSLDNWSV 133

Query: 160 RWCFSCNTLIPVEKTEENADQKDGLSNVVKLIRDRSME--GSPVDVEN--TWRASSEVTA 219
            WC+ C+  +    + +     D +     +   +  E     +++EN    + S     
Sbjct: 134 -WCYICDNEVQYCSSNQLGQVVDYVRKQASITTPKPAEKDNGNIELENKKLEKESKNEQE 193

Query: 220 ATKSESSILSD--LDRRNHYVVKGLINLGNTCFFNSILQNLLAIDMLRDY---------F 279
             K E+    +  ++  +   VKGL NLGNTCFFN+++QNL    +LR+           
Sbjct: 194 REKKENMAKENPPMNSPSQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKEVKMSGTI 253

Query: 280 LKLE--------------ECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGCISSKAPQ 339
           +K+E              E  GPLT+A+ +   E  M+   KG + P+ +F  +  KA +
Sbjct: 254 VKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNE--MQETKKGIVTPKELFSQVCKKAVR 313

Query: 340 FKGYDQHDSHELLRVLLDGL------------------STEELASRKMTKSKEEKTSGNS 399
           FKGY Q DS ELLR LLDG+                  STE+L      K K+ +    S
Sbjct: 314 FKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEELKNKVKDYEKK-KS 373

Query: 400 TPTFVDEMFGGQISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKV 459
            P+FVD +FGG+++S + C +C   S V+E FLDLSLPV +     KK       KKT  
Sbjct: 374 IPSFVDRIFGGELTSTIMCDQCRTVSLVHESFLDLSLPV-LDDQSGKKSVNDKNLKKTME 433

Query: 460 PPKRSGKTFPKTEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDV 519
              +      + EK +D    + + +PS              GTS  ++K    Q     
Sbjct: 434 DEDQDS----EEEKDNDSYIKERSDIPS--------------GTSKHLQKKAKKQAKKQA 493

Query: 520 KESEKEVLIENGGECTSDSLTWLDFLGPEPNAENCNTS-----------TTQDSENNVEV 579
           K   ++  I+ G     + +  +D   PE N      S            +Q+   + E 
Sbjct: 494 KNQRRQQKIQ-GKVLHLNDICTIDH--PEDNEYEAEMSLQGEVNIKSNHISQEGVMHKEY 553

Query: 580 FINDDSQQSISGSSNQGSSL--HNEPSQQPDFSSINLWDDESPLQVQASEV---LLLPYK 639
            +N   Q+ ++G      S+  + +P+++ D  +IN+ +D   L    +E    L   Y 
Sbjct: 554 CVN---QKDLNGQEKMIESVTDNQKPTEEVDMKNINMDNDLEVLTSSPTECTRNLNGAYL 613

Query: 640 EESSTTEVAKEDDQASSSILGCAQDDLDGFGLGDMFDEPEITVGPLPRPSTVNEVSESSF 699
            E+S  EV   +   + ++      D     + +    P   V        VNE  E++F
Sbjct: 614 TEASNGEVDISNGFKNLNLNAALHPDEINIEILNDSHTPGTKV-----YEVVNEDPETAF 673

Query: 700 ISESDPDEVDNSDSPVSVESCLTYFTKPELLSNENGYNCEKCLKRLQQQRLEMKKQSKVA 759
            + ++  EV N+D   S++ CL  FT+ E L + N   CE C +R               
Sbjct: 674 CTLAN-REVFNTDE-CSIQHCLYQFTRNEKLRDANKLLCEVCTRR--------------- 733

Query: 760 REVVVNGCQTAVGGDISCLNENSSVEVKNQRNVSLVNGSMCYSSGESMNLHENVDCSSQV 819
                            C                  NG      GE  +++ N       
Sbjct: 734 ----------------QC------------------NGPKANMKGERKHVYTN------- 793

Query: 820 CTKPADCQTGKLNSLVLDENEAKVDGDVNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDS 879
                                                                       
Sbjct: 794 ------------------------------------------------------------ 823

Query: 880 RGSGLAKSNIEPLSSQISVENSELEKSEDGEMDSDSTIVNVKRDATKRFLIHKAPPILTI 939
                                                       A K+ LI  APP+LT+
Sbjct: 854 --------------------------------------------AKKQMLISLAPPVLTL 823

Query: 940 HIKRFSPDARGRYSKLNGHVRFKETIDLKPYMDTRC----EDRDKCSYRLIGVVEHSGSM 985
           H+KRF   A     K+N H++F E +DL P+   +C    E+  +  Y L GVVEHSG+M
Sbjct: 914 HLKRFQ-QAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEENTRVLYSLYGVVEHSGTM 823

BLAST of MC05g0186 vs. ExPASy Swiss-Prot
Match: Q9Y5T5 (Ubiquitin carboxyl-terminal hydrolase 16 OS=Homo sapiens OX=9606 GN=USP16 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.5e-45
Identity = 243/1023 (23.75%), Postives = 383/1023 (37.44%), Query Frame = 0

Query: 40  DDASVVKEKRECRHLDKCLNLSELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKG 99
           DD+S   E   CRH+ K L    L   + + E   C+DC+  +      K KA+      
Sbjct: 14  DDSSETLEP-VCRHIRKGLEQGNLKKALVNVEWNICQDCKTDNK----VKDKAE------ 73

Query: 100 GAPVDVKSDSKAIWICLQCGHYACGGIGLPTNSQSHAVRHV---RQTRHPVVIQFENPQL 159
               +   +  ++W+CL+CGH  CG      + + HA++H    R   H +V+  +N  +
Sbjct: 74  ----EETEEKPSVWLCLKCGHQGCG----RNSQEQHALKHYLTPRSEPHCLVLSLDNWSV 133

Query: 160 RWCFSCNTLIPVEKTEENADQKDGLSNVVKLIRDRSME--GSPVDVENTWRASSEVTAAT 219
            WC+ C+  +    + +     D +     +   +  E     +++EN      E  +  
Sbjct: 134 -WCYVCDNEVQYCSSNQLGQVVDYVRKQASITTPKPAEKDNGNIELEN---KKLEKESKN 193

Query: 220 KSESSILSDLDRRN-------HYVVKGLINLGNTCFFNSILQNLLAIDMLRDY------- 279
           + E     ++ + N          VKGL NLGNTCFFN+++QNL    +LR+        
Sbjct: 194 EQEREKKENMAKENPPMNSPCQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKEVKMS 253

Query: 280 --FLKLE--------------ECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGCISSK 339
              +K+E              E  GPLT+A+ +   E  M+   KG + P+ +F  +  K
Sbjct: 254 GTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNE--MQETKKGVVTPKELFSQVCKK 313

Query: 340 APQFKGYDQHDSHELLRVLLDGL------------------STEELASRKMTKSKEEKTS 399
           A +FKGY Q DS ELLR LLDG+                  STE+L      K K+ +  
Sbjct: 314 AVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEELKNKVKDYEKK 373

Query: 400 GNSTPTFVDEMFGGQISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKK 459
             S P+FVD +FGG+++S + C +C   S V+E FLDLSLPV +     KK       KK
Sbjct: 374 -KSMPSFVDRIFGGELTSMIMCDQCRTVSLVHESFLDLSLPV-LDDQSGKKSVNDKNLKK 433

Query: 460 TKVPPKRSGKTFPKTEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNV 519
           T     +      + EK +D    + + +PS              GTS  ++K    Q  
Sbjct: 434 TVEDEDQDS----EEEKDNDSYIKERSDIPS--------------GTSKHLQKKAKKQAK 493

Query: 520 SDVKESEKEVLIEN-----GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNV---EV 579
              K   ++  I+         CT D     ++          N  +   S+  V   E 
Sbjct: 494 KQAKNQRRQQKIQGKVLHLNDICTIDHPEDSEYEAEMSLQGEVNIKSNHISQEGVMHKEY 553

Query: 580 FINDDSQQSISGSSNQGSSL--HNEPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEES 639
            +N   Q+ ++G +    S+  + + +++ D  +IN+ +D   L    +  L   Y  E 
Sbjct: 554 CVN---QKDLNGQAKMIESVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLTEG 613

Query: 640 STTEVAKEDDQASSSILGCAQDDLDGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISE 699
           S  EV   +   + ++      D     + +    P   V        VNE  E++F + 
Sbjct: 614 SNGEVDISNGFKNLNLNAALHPDEINIEILNDSHTPGTKV-----YEVVNEDPETAFCTL 673

Query: 700 SDPDEVDNSDSPVSVESCLTYFTKPELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREV 759
           ++  EV N+D   S++ CL  FT+ E L + N   CE C +R                  
Sbjct: 674 AN-REVFNTDE-CSIQHCLYQFTRNEKLRDANKLLCEVCTRR------------------ 733

Query: 760 VVNGCQTAVGGDISCLNENSSVEVKNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTK 819
                         C                  NG      GE  +++ N          
Sbjct: 734 -------------QC------------------NGPKANIKGERKHVYTN---------- 793

Query: 820 PADCQTGKLNSLVLDENEAKVDGDVNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGS 879
                                                                       
Sbjct: 794 ------------------------------------------------------------ 820

Query: 880 GLAKSNIEPLSSQISVENSELEKSEDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIK 939
                                                    A K+ LI  APP+LT+H+K
Sbjct: 854 -----------------------------------------AKKQMLISLAPPVLTLHLK 820

Query: 940 RFSPDARGRYSKLNGHVRFKETIDLKPYMDTRC----EDRDKCSYRLIGVVEHSGSMRGG 985
           RF   A     K+N H++F E +DL P+   +C    E+  +  Y L GVVEHSG+MR G
Sbjct: 914 RFQ-QAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEENTRVLYSLYGVVEHSGTMRSG 820

BLAST of MC05g0186 vs. NCBI nr
Match: XP_022146980.1 (ubiquitin carboxyl-terminal hydrolase 2 [Momordica charantia])

HSP 1 Score: 1910 bits (4948), Expect = 0.0
Identity = 986/986 (100.00%), Postives = 986/986 (100.00%), Query Frame = 0

Query: 1   MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
           MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL
Sbjct: 1   MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60

Query: 61  SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
           SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH
Sbjct: 61  SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120

Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
           YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG
Sbjct: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180

Query: 181 LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240
           LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN
Sbjct: 181 LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240

Query: 241 TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300
           TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC
Sbjct: 241 TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300

Query: 301 ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360
           ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG
Sbjct: 301 ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360

Query: 361 QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420
           QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK
Sbjct: 361 QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420

Query: 421 TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480
           TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN
Sbjct: 421 TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480

Query: 481 GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540
           GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN
Sbjct: 481 GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540

Query: 541 EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600
           EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL
Sbjct: 541 EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600

Query: 601 DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660
           DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK
Sbjct: 601 DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660

Query: 661 PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720
           PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV
Sbjct: 661 PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720

Query: 721 KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780
           KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD
Sbjct: 721 KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780

Query: 781 VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840
           VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS
Sbjct: 781 VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840

Query: 841 EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900
           EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID
Sbjct: 841 EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900

Query: 901 LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960
           LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA
Sbjct: 901 LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960

Query: 961 SDACVNEVTLDRVLCCEAYILFYEIT 986
           SDACVNEVTLDRVLCCEAYILFYEIT
Sbjct: 961 SDACVNEVTLDRVLCCEAYILFYEIT 986

BLAST of MC05g0186 vs. NCBI nr
Match: XP_038900463.1 (ubiquitin carboxyl-terminal hydrolase 2 [Benincasa hispida] >XP_038900471.1 ubiquitin carboxyl-terminal hydrolase 2 [Benincasa hispida])

HSP 1 Score: 1595 bits (4130), Expect = 0.0
Identity = 816/986 (82.76%), Postives = 899/986 (91.18%), Query Frame = 0

Query: 1   MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
           MGKKVKKK NRAPPKEKRVSS SP  VPQ++STTVE A +D  VVKEK +CRHLDKC NL
Sbjct: 1   MGKKVKKKTNRAPPKEKRVSSSSPRKVPQRNSTTVETADEDVPVVKEKSQCRHLDKCFNL 60

Query: 61  SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
           +ELSSK+GSSEPI+CEDCQ+SSADRRG++GKA+HGKKKGG  VDVK+D+KAIWICLQCGH
Sbjct: 61  NELSSKLGSSEPISCEDCQDSSADRRGTRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120

Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
           YACGGIGLPTN QSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTL+PVEKTEEN +QKD 
Sbjct: 121 YACGGIGLPTNPQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDD 180

Query: 181 LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240
           LS+VVKLIRDRSME SPVDV+NTW  SSEVTA T+SESS+LSDLDRRNHYVVKGLINLGN
Sbjct: 181 LSDVVKLIRDRSMESSPVDVKNTWHTSSEVTAGTESESSVLSDLDRRNHYVVKGLINLGN 240

Query: 241 TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300
           TCFFNSILQNLLAIDMLRD+F KLEECVGPLTIALKK+FIE RME ++K SINPRSVFG 
Sbjct: 241 TCFFNSILQNLLAIDMLRDHFTKLEECVGPLTIALKKIFIEARMETRMKCSINPRSVFGS 300

Query: 301 ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360
           ISSKAPQFKGY+QHDSHELLRVLLD LS+EEL SRKMT SKEE+ SGN TPTFVDEMFGG
Sbjct: 301 ISSKAPQFKGYEQHDSHELLRVLLDTLSSEELTSRKMTNSKEERISGNPTPTFVDEMFGG 360

Query: 361 QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420
           QISSAVCC ECGH STVYEPFLDLSLPVP+KK LAKKVQP SRAKKTK+PPKR+GKT PK
Sbjct: 361 QISSAVCCKECGHISTVYEPFLDLSLPVPMKKSLAKKVQPASRAKKTKLPPKRNGKTIPK 420

Query: 421 TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480
           T+KASDVV+VQIASVPSSS+ESSLP+EA    T+TIMEKTL+SQNVSDVKESEKEVLIEN
Sbjct: 421 TDKASDVVTVQIASVPSSSNESSLPSEAGVSSTTTIMEKTLTSQNVSDVKESEKEVLIEN 480

Query: 481 GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540
           GGEC SD+LTW+DFLGPE NA+NC+ ST QDSENNVE+FI+DDSQQS+SG S Q SSLHN
Sbjct: 481 GGECASDNLTWMDFLGPELNAKNCDISTIQDSENNVELFISDDSQQSVSGLSMQVSSLHN 540

Query: 541 EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600
           EP+Q+PDFSS+N WD E+PLQVQASEVLLLPYKEESST E+AKEDDQASSSILGCAQ+D 
Sbjct: 541 EPNQRPDFSSVNSWDAEAPLQVQASEVLLLPYKEESSTAELAKEDDQASSSILGCAQEDF 600

Query: 601 DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660
           DGFGLGDMFDEPEI +GP+ RPS  NEV+ESSF SESD +EVDN+D+PVSVESCLTYFTK
Sbjct: 601 DGFGLGDMFDEPEIAIGPIARPSMSNEVAESSFNSESDHEEVDNTDAPVSVESCLTYFTK 660

Query: 661 PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720
           PELLSNENGYNCEKC KRLQ+Q+LEMK+QSKVA + V NGCQTA+GGD S  NE+SSVEV
Sbjct: 661 PELLSNENGYNCEKCSKRLQRQKLEMKRQSKVACKAVANGCQTAIGGDTSSFNEDSSVEV 720

Query: 721 KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780
           KNQRNV+L NGS+  +SGES+NL EN DCSS+ CTKPA+CQ GK +SL LDE++AKVD D
Sbjct: 721 KNQRNVNLANGSVSNNSGESLNLKENADCSSRDCTKPANCQKGKTDSLPLDEDDAKVDTD 780

Query: 781 VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840
           +N G AYSSGC+NTSN+ENSDDRS  SLSN+D + S LAK+N E LSSQIS EN + EKS
Sbjct: 781 MNPGLAYSSGCNNTSNQENSDDRSGCSLSNDDPKSSALAKTNTELLSSQISAEN-QSEKS 840

Query: 841 EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900
           EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGH+RFKETID
Sbjct: 841 EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHIRFKETID 900

Query: 901 LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960
           LKPY+DTRC DRDKC+YRL+GVVEHSGSMRGGHYVAYVRGG+R+RS+GEAEEDASVWYYA
Sbjct: 901 LKPYLDTRCVDRDKCNYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSNGEAEEDASVWYYA 960

Query: 961 SDACVNEVTLDRVLCCEAYILFYEIT 986
           SDA VNEVTLDRVL CEAYILFYEIT
Sbjct: 961 SDAVVNEVTLDRVLGCEAYILFYEIT 985

BLAST of MC05g0186 vs. NCBI nr
Match: KAA0049148.1 (ubiquitin carboxyl-terminal hydrolase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1573 bits (4073), Expect = 0.0
Identity = 807/986 (81.85%), Postives = 892/986 (90.47%), Query Frame = 0

Query: 1    MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
            MGKKVKKK NRAPPKEKRVSS SP  VPQ S+TTVE A ++ S+VKEK +CRHLDKC NL
Sbjct: 606  MGKKVKKKTNRAPPKEKRVSSNSPRKVPQPSNTTVETADEEVSIVKEKSQCRHLDKCFNL 665

Query: 61   SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
            +ELSSK+GS+EPI+CEDCQ+SSADRRGS+GKA+HGKKKGG  VDVK+D+KAIWICLQCGH
Sbjct: 666  NELSSKLGSAEPISCEDCQDSSADRRGSRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 725

Query: 121  YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
            YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTL+PVEKTEEN +QKDG
Sbjct: 726  YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDG 785

Query: 181  LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240
            LSNVVKLIRDRSME +PVDVENT  AS+EVTAATKSESS+L DLDRRN YVVKGLINLGN
Sbjct: 786  LSNVVKLIRDRSMESTPVDVENTRYASNEVTAATKSESSVLIDLDRRNQYVVKGLINLGN 845

Query: 241  TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300
            TCFFNSILQNLLAIDMLRD+F+KLEEC+GPLTIALKK+FIE R E ++KGSINPRSVFGC
Sbjct: 846  TCFFNSILQNLLAIDMLRDHFVKLEECIGPLTIALKKIFIEARTESRMKGSINPRSVFGC 905

Query: 301  ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360
            ISSKAPQFKGY+QHDSHELLRVLLD LS+EEL SRKMT SKEE+ SGN TPTFVDEMFGG
Sbjct: 906  ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNLTPTFVDEMFGG 965

Query: 361  QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420
            QISSAVCC ECGHTSTVYEPFLDLSLPVP+KKPLAKKVQPVSRAKKTKVPPKR+GKT PK
Sbjct: 966  QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 1025

Query: 421  TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480
            T K SD+V VQIASVPSSS+ESSLP+EA +  T+TIMEKT +SQNVSD K+SEKE+ +EN
Sbjct: 1026 TNKVSDIVPVQIASVPSSSNESSLPSEACASSTTTIMEKTSTSQNVSDAKKSEKEISVEN 1085

Query: 481  GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540
            GGEC SD+LTWLDFL PE NA+NC+ ST +DSENNVEVFI+DDSQQS+SG S   SS+HN
Sbjct: 1086 GGECASDNLTWLDFLEPELNADNCDISTARDSENNVEVFISDDSQQSVSGLSMPVSSIHN 1145

Query: 541  EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600
            EP+Q+PDFSS+N WDDE+PLQVQASEVLLLPYKEESS+ EV KEDDQASSSILGCAQ+D 
Sbjct: 1146 EPNQRPDFSSVNSWDDEAPLQVQASEVLLLPYKEESSSAEVVKEDDQASSSILGCAQEDF 1205

Query: 601  DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660
            DGFGLGDMFDEPEI +GP+ RPST NEV+ESSF SESDP+EVDN+D+PVSVESCLT+FTK
Sbjct: 1206 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 1265

Query: 661  PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720
            PELLSNENGYNCEKC KRLQQQRLEMKKQSKVA + V NGCQTAVGGDIS  NENSSVEV
Sbjct: 1266 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNENSSVEV 1325

Query: 721  KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780
            KNQRN +L NGS+ Y+SGES NL +N DCSSQ CTKP +CQ GK + LVLDE+EAKVD D
Sbjct: 1326 KNQRNTNLTNGSIIYNSGESSNLKKNADCSSQDCTKPVNCQIGKTDPLVLDEDEAKVDKD 1385

Query: 781  VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840
            +N G + SSGCSNT N+ENSDD+SS SL N++      AK+ IE LSS +SV N + E+S
Sbjct: 1386 MNPGLSCSSGCSNTCNQENSDDKSS-SLPNDEH-----AKTKIEHLSSHLSVGN-QSEES 1445

Query: 841  EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900
            EDGEMDSD T+VNVKRDATKRFLIHKAPPILTIH+KRF+PDARGRYSKLNGHVRFKETID
Sbjct: 1446 EDGEMDSDPTVVNVKRDATKRFLIHKAPPILTIHMKRFTPDARGRYSKLNGHVRFKETID 1505

Query: 901  LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960
            LKPY+DTRC DRDKC+YRL+GVVEHSGSMRGGHYVAYVRGG+R+RSSGEAEEDASVWYYA
Sbjct: 1506 LKPYVDTRCADRDKCNYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYA 1565

Query: 961  SDACVNEVTLDRVLCCEAYILFYEIT 986
            SDA VNEVTLDRVL CEAYILFYEIT
Sbjct: 1566 SDAVVNEVTLDRVLGCEAYILFYEIT 1584

BLAST of MC05g0186 vs. NCBI nr
Match: TYK17412.1 (ubiquitin carboxyl-terminal hydrolase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1573 bits (4073), Expect = 0.0
Identity = 807/986 (81.85%), Postives = 892/986 (90.47%), Query Frame = 0

Query: 1   MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
           MGKKVKKK NRAPPKEKRVSS SP  VPQ S+TTVE A ++ S+VKEK +CRHLDKC NL
Sbjct: 1   MGKKVKKKTNRAPPKEKRVSSNSPRKVPQPSNTTVETADEEVSIVKEKSQCRHLDKCFNL 60

Query: 61  SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
           +ELSSK+GS+EPI+CEDCQ+SSADRRGS+GKA+HGKKKGG  VDVK+D+KAIWICLQCGH
Sbjct: 61  NELSSKLGSAEPISCEDCQDSSADRRGSRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120

Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
           YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTL+PVEKTEEN +QKDG
Sbjct: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDG 180

Query: 181 LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240
           LSNVVKLIRDRSME +PVDVENT  AS+EVTAATKSESS+L DLDRRN YVVKGLINLGN
Sbjct: 181 LSNVVKLIRDRSMESTPVDVENTRYASNEVTAATKSESSVLIDLDRRNQYVVKGLINLGN 240

Query: 241 TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300
           TCFFNSILQNLLAIDMLRD+F+KLEEC+GPLTIALKK+FIE R E ++KGSINPRSVFGC
Sbjct: 241 TCFFNSILQNLLAIDMLRDHFVKLEECIGPLTIALKKIFIEARTESRMKGSINPRSVFGC 300

Query: 301 ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360
           ISSKAPQFKGY+QHDSHELLRVLLD LS+EEL SRKMT SKEE+ SGN TPTFVDEMFGG
Sbjct: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNLTPTFVDEMFGG 360

Query: 361 QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420
           QISSAVCC ECGHTSTVYEPFLDLSLPVP+KKPLAKKVQPVSRAKKTKVPPKR+GKT PK
Sbjct: 361 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 420

Query: 421 TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480
           T K SD+V VQIASVPSSS+ESSLP+EA +  T+TIMEKT +SQNVSD K+SEKE+ +EN
Sbjct: 421 TNKVSDIVPVQIASVPSSSNESSLPSEACASSTTTIMEKTSTSQNVSDAKKSEKEISVEN 480

Query: 481 GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540
           GGEC SD+LTWLDFL PE NA+NC+ ST +DSENNVEVFI+DDSQQS+SG S   SS+HN
Sbjct: 481 GGECASDNLTWLDFLEPELNADNCDISTARDSENNVEVFISDDSQQSVSGLSMPVSSIHN 540

Query: 541 EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600
           EP+Q+PDFSS+N WDDE+PLQVQASEVLLLPYKEESS+ EV KEDDQASSSILGCAQ+D 
Sbjct: 541 EPNQRPDFSSVNSWDDEAPLQVQASEVLLLPYKEESSSAEVVKEDDQASSSILGCAQEDF 600

Query: 601 DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660
           DGFGLGDMFDEPEI +GP+ RPST NEV+ESSF SESDP+EVDN+D+PVSVESCLT+FTK
Sbjct: 601 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 660

Query: 661 PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720
           PELLSNENGYNCEKC KRLQQQRLEMKKQSKVA + V NGCQTAVGGDIS  NENSSVEV
Sbjct: 661 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNENSSVEV 720

Query: 721 KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780
           KNQRN +L NGS+ Y+SGES NL +N DCSSQ CTKP +CQ GK + LVLDE+EAKVD D
Sbjct: 721 KNQRNTNLTNGSIIYNSGESSNLKKNADCSSQDCTKPVNCQIGKTDPLVLDEDEAKVDKD 780

Query: 781 VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840
           +N G + SSGCSNT N+ENSDD+SS SL N++      AK+ IE LSS +SV N + E+S
Sbjct: 781 MNPGLSCSSGCSNTCNQENSDDKSS-SLPNDEH-----AKTKIEHLSSHLSVGN-QSEES 840

Query: 841 EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900
           EDGEMDSD T+VNVKRDATKRFLIHKAPPILTIH+KRF+PDARGRYSKLNGHVRFKETID
Sbjct: 841 EDGEMDSDPTVVNVKRDATKRFLIHKAPPILTIHMKRFTPDARGRYSKLNGHVRFKETID 900

Query: 901 LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960
           LKPY+DTRC DRDKC+YRL+GVVEHSGSMRGGHYVAYVRGG+R+RSSGEAEEDASVWYYA
Sbjct: 901 LKPYVDTRCADRDKCNYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYA 960

Query: 961 SDACVNEVTLDRVLCCEAYILFYEIT 986
           SDA VNEVTLDRVL CEAYILFYEIT
Sbjct: 961 SDAVVNEVTLDRVLGCEAYILFYEIT 979

BLAST of MC05g0186 vs. NCBI nr
Match: XP_016898951.1 (PREDICTED: ubiquitin carboxyl-terminal hydrolase 2 [Cucumis melo])

HSP 1 Score: 1573 bits (4073), Expect = 0.0
Identity = 807/986 (81.85%), Postives = 892/986 (90.47%), Query Frame = 0

Query: 1    MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
            MGKKVKKK NRAPPKEKRVSS SP  VPQ S+TTVE A ++ S+VKEK +CRHLDKC NL
Sbjct: 709  MGKKVKKKTNRAPPKEKRVSSNSPRKVPQPSNTTVETADEEVSIVKEKSQCRHLDKCFNL 768

Query: 61   SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
            +ELSSK+GS+EPI+CEDCQ+SSADRRGS+GKA+HGKKKGG  VDVK+D+KAIWICLQCGH
Sbjct: 769  NELSSKLGSAEPISCEDCQDSSADRRGSRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 828

Query: 121  YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
            YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTL+PVEKTEEN +QKDG
Sbjct: 829  YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDG 888

Query: 181  LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240
            LSNVVKLIRDRSME +PVDVENT  AS+EVTAATKSESS+L DLDRRN YVVKGLINLGN
Sbjct: 889  LSNVVKLIRDRSMESTPVDVENTRYASNEVTAATKSESSVLIDLDRRNQYVVKGLINLGN 948

Query: 241  TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300
            TCFFNSILQNLLAIDMLRD+F+KLEEC+GPLTIALKK+FIE R E ++KGSINPRSVFGC
Sbjct: 949  TCFFNSILQNLLAIDMLRDHFVKLEECIGPLTIALKKIFIEARTESRMKGSINPRSVFGC 1008

Query: 301  ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360
            ISSKAPQFKGY+QHDSHELLRVLLD LS+EEL SRKMT SKEE+ SGN TPTFVDEMFGG
Sbjct: 1009 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNLTPTFVDEMFGG 1068

Query: 361  QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420
            QISSAVCC ECGHTSTVYEPFLDLSLPVP+KKPLAKKVQPVSRAKKTKVPPKR+GKT PK
Sbjct: 1069 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 1128

Query: 421  TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480
            T K SD+V VQIASVPSSS+ESSLP+EA +  T+TIMEKT +SQNVSD K+SEKE+ +EN
Sbjct: 1129 TNKVSDIVPVQIASVPSSSNESSLPSEACASSTTTIMEKTSTSQNVSDAKKSEKEISVEN 1188

Query: 481  GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540
            GGEC SD+LTWLDFL PE NA+NC+ ST +DSENNVEVFI+DDSQQS+SG S   SS+HN
Sbjct: 1189 GGECASDNLTWLDFLEPELNADNCDISTARDSENNVEVFISDDSQQSVSGLSMPVSSIHN 1248

Query: 541  EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600
            EP+Q+PDFSS+N WDDE+PLQVQASEVLLLPYKEESS+ EV KEDDQASSSILGCAQ+D 
Sbjct: 1249 EPNQRPDFSSVNSWDDEAPLQVQASEVLLLPYKEESSSAEVVKEDDQASSSILGCAQEDF 1308

Query: 601  DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660
            DGFGLGDMFDEPEI +GP+ RPST NEV+ESSF SESDP+EVDN+D+PVSVESCLT+FTK
Sbjct: 1309 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 1368

Query: 661  PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720
            PELLSNENGYNCEKC KRLQQQRLEMKKQSKVA + V NGCQTAVGGDIS  NENSSVEV
Sbjct: 1369 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNENSSVEV 1428

Query: 721  KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780
            KNQRN +L NGS+ Y+SGES NL +N DCSSQ CTKP +CQ GK + LVLDE+EAKVD D
Sbjct: 1429 KNQRNTNLTNGSIIYNSGESSNLKKNADCSSQDCTKPVNCQIGKTDPLVLDEDEAKVDKD 1488

Query: 781  VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840
            +N G + SSGCSNT N+ENSDD+SS SL N++      AK+ IE LSS +SV N + E+S
Sbjct: 1489 MNPGLSCSSGCSNTCNQENSDDKSS-SLPNDEH-----AKTKIEHLSSHLSVGN-QSEES 1548

Query: 841  EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900
            EDGEMDSD T+VNVKRDATKRFLIHKAPPILTIH+KRF+PDARGRYSKLNGHVRFKETID
Sbjct: 1549 EDGEMDSDPTVVNVKRDATKRFLIHKAPPILTIHMKRFTPDARGRYSKLNGHVRFKETID 1608

Query: 901  LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960
            LKPY+DTRC DRDKC+YRL+GVVEHSGSMRGGHYVAYVRGG+R+RSSGEAEEDASVWYYA
Sbjct: 1609 LKPYVDTRCADRDKCNYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYA 1668

Query: 961  SDACVNEVTLDRVLCCEAYILFYEIT 986
            SDA VNEVTLDRVL CEAYILFYEIT
Sbjct: 1669 SDAVVNEVTLDRVLGCEAYILFYEIT 1687

BLAST of MC05g0186 vs. ExPASy TrEMBL
Match: A0A6J1CYV7 (Ubiquitin carboxyl-terminal hydrolase OS=Momordica charantia OX=3673 GN=LOC111016042 PE=3 SV=1)

HSP 1 Score: 1910 bits (4948), Expect = 0.0
Identity = 986/986 (100.00%), Postives = 986/986 (100.00%), Query Frame = 0

Query: 1   MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
           MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL
Sbjct: 1   MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60

Query: 61  SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
           SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH
Sbjct: 61  SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120

Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
           YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG
Sbjct: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180

Query: 181 LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240
           LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN
Sbjct: 181 LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240

Query: 241 TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300
           TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC
Sbjct: 241 TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300

Query: 301 ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360
           ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG
Sbjct: 301 ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360

Query: 361 QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420
           QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK
Sbjct: 361 QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420

Query: 421 TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480
           TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN
Sbjct: 421 TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480

Query: 481 GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540
           GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN
Sbjct: 481 GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540

Query: 541 EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600
           EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL
Sbjct: 541 EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600

Query: 601 DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660
           DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK
Sbjct: 601 DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660

Query: 661 PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720
           PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV
Sbjct: 661 PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720

Query: 721 KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780
           KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD
Sbjct: 721 KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780

Query: 781 VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840
           VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS
Sbjct: 781 VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840

Query: 841 EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900
           EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID
Sbjct: 841 EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900

Query: 901 LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960
           LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA
Sbjct: 901 LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960

Query: 961 SDACVNEVTLDRVLCCEAYILFYEIT 986
           SDACVNEVTLDRVLCCEAYILFYEIT
Sbjct: 961 SDACVNEVTLDRVLCCEAYILFYEIT 986

BLAST of MC05g0186 vs. ExPASy TrEMBL
Match: A0A5D3D0Q5 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002740 PE=3 SV=1)

HSP 1 Score: 1573 bits (4073), Expect = 0.0
Identity = 807/986 (81.85%), Postives = 892/986 (90.47%), Query Frame = 0

Query: 1   MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
           MGKKVKKK NRAPPKEKRVSS SP  VPQ S+TTVE A ++ S+VKEK +CRHLDKC NL
Sbjct: 1   MGKKVKKKTNRAPPKEKRVSSNSPRKVPQPSNTTVETADEEVSIVKEKSQCRHLDKCFNL 60

Query: 61  SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
           +ELSSK+GS+EPI+CEDCQ+SSADRRGS+GKA+HGKKKGG  VDVK+D+KAIWICLQCGH
Sbjct: 61  NELSSKLGSAEPISCEDCQDSSADRRGSRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 120

Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
           YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTL+PVEKTEEN +QKDG
Sbjct: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDG 180

Query: 181 LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240
           LSNVVKLIRDRSME +PVDVENT  AS+EVTAATKSESS+L DLDRRN YVVKGLINLGN
Sbjct: 181 LSNVVKLIRDRSMESTPVDVENTRYASNEVTAATKSESSVLIDLDRRNQYVVKGLINLGN 240

Query: 241 TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300
           TCFFNSILQNLLAIDMLRD+F+KLEEC+GPLTIALKK+FIE R E ++KGSINPRSVFGC
Sbjct: 241 TCFFNSILQNLLAIDMLRDHFVKLEECIGPLTIALKKIFIEARTESRMKGSINPRSVFGC 300

Query: 301 ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360
           ISSKAPQFKGY+QHDSHELLRVLLD LS+EEL SRKMT SKEE+ SGN TPTFVDEMFGG
Sbjct: 301 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNLTPTFVDEMFGG 360

Query: 361 QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420
           QISSAVCC ECGHTSTVYEPFLDLSLPVP+KKPLAKKVQPVSRAKKTKVPPKR+GKT PK
Sbjct: 361 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 420

Query: 421 TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480
           T K SD+V VQIASVPSSS+ESSLP+EA +  T+TIMEKT +SQNVSD K+SEKE+ +EN
Sbjct: 421 TNKVSDIVPVQIASVPSSSNESSLPSEACASSTTTIMEKTSTSQNVSDAKKSEKEISVEN 480

Query: 481 GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540
           GGEC SD+LTWLDFL PE NA+NC+ ST +DSENNVEVFI+DDSQQS+SG S   SS+HN
Sbjct: 481 GGECASDNLTWLDFLEPELNADNCDISTARDSENNVEVFISDDSQQSVSGLSMPVSSIHN 540

Query: 541 EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600
           EP+Q+PDFSS+N WDDE+PLQVQASEVLLLPYKEESS+ EV KEDDQASSSILGCAQ+D 
Sbjct: 541 EPNQRPDFSSVNSWDDEAPLQVQASEVLLLPYKEESSSAEVVKEDDQASSSILGCAQEDF 600

Query: 601 DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660
           DGFGLGDMFDEPEI +GP+ RPST NEV+ESSF SESDP+EVDN+D+PVSVESCLT+FTK
Sbjct: 601 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 660

Query: 661 PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720
           PELLSNENGYNCEKC KRLQQQRLEMKKQSKVA + V NGCQTAVGGDIS  NENSSVEV
Sbjct: 661 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNENSSVEV 720

Query: 721 KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780
           KNQRN +L NGS+ Y+SGES NL +N DCSSQ CTKP +CQ GK + LVLDE+EAKVD D
Sbjct: 721 KNQRNTNLTNGSIIYNSGESSNLKKNADCSSQDCTKPVNCQIGKTDPLVLDEDEAKVDKD 780

Query: 781 VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840
           +N G + SSGCSNT N+ENSDD+SS SL N++      AK+ IE LSS +SV N + E+S
Sbjct: 781 MNPGLSCSSGCSNTCNQENSDDKSS-SLPNDEH-----AKTKIEHLSSHLSVGN-QSEES 840

Query: 841 EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900
           EDGEMDSD T+VNVKRDATKRFLIHKAPPILTIH+KRF+PDARGRYSKLNGHVRFKETID
Sbjct: 841 EDGEMDSDPTVVNVKRDATKRFLIHKAPPILTIHMKRFTPDARGRYSKLNGHVRFKETID 900

Query: 901 LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960
           LKPY+DTRC DRDKC+YRL+GVVEHSGSMRGGHYVAYVRGG+R+RSSGEAEEDASVWYYA
Sbjct: 901 LKPYVDTRCADRDKCNYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYA 960

Query: 961 SDACVNEVTLDRVLCCEAYILFYEIT 986
           SDA VNEVTLDRVL CEAYILFYEIT
Sbjct: 961 SDAVVNEVTLDRVLGCEAYILFYEIT 979

BLAST of MC05g0186 vs. ExPASy TrEMBL
Match: A0A5A7U1P6 (Ubiquitinyl hydrolase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G003810 PE=3 SV=1)

HSP 1 Score: 1573 bits (4073), Expect = 0.0
Identity = 807/986 (81.85%), Postives = 892/986 (90.47%), Query Frame = 0

Query: 1    MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
            MGKKVKKK NRAPPKEKRVSS SP  VPQ S+TTVE A ++ S+VKEK +CRHLDKC NL
Sbjct: 606  MGKKVKKKTNRAPPKEKRVSSNSPRKVPQPSNTTVETADEEVSIVKEKSQCRHLDKCFNL 665

Query: 61   SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
            +ELSSK+GS+EPI+CEDCQ+SSADRRGS+GKA+HGKKKGG  VDVK+D+KAIWICLQCGH
Sbjct: 666  NELSSKLGSAEPISCEDCQDSSADRRGSRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 725

Query: 121  YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
            YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTL+PVEKTEEN +QKDG
Sbjct: 726  YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDG 785

Query: 181  LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240
            LSNVVKLIRDRSME +PVDVENT  AS+EVTAATKSESS+L DLDRRN YVVKGLINLGN
Sbjct: 786  LSNVVKLIRDRSMESTPVDVENTRYASNEVTAATKSESSVLIDLDRRNQYVVKGLINLGN 845

Query: 241  TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300
            TCFFNSILQNLLAIDMLRD+F+KLEEC+GPLTIALKK+FIE R E ++KGSINPRSVFGC
Sbjct: 846  TCFFNSILQNLLAIDMLRDHFVKLEECIGPLTIALKKIFIEARTESRMKGSINPRSVFGC 905

Query: 301  ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360
            ISSKAPQFKGY+QHDSHELLRVLLD LS+EEL SRKMT SKEE+ SGN TPTFVDEMFGG
Sbjct: 906  ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNLTPTFVDEMFGG 965

Query: 361  QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420
            QISSAVCC ECGHTSTVYEPFLDLSLPVP+KKPLAKKVQPVSRAKKTKVPPKR+GKT PK
Sbjct: 966  QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 1025

Query: 421  TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480
            T K SD+V VQIASVPSSS+ESSLP+EA +  T+TIMEKT +SQNVSD K+SEKE+ +EN
Sbjct: 1026 TNKVSDIVPVQIASVPSSSNESSLPSEACASSTTTIMEKTSTSQNVSDAKKSEKEISVEN 1085

Query: 481  GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540
            GGEC SD+LTWLDFL PE NA+NC+ ST +DSENNVEVFI+DDSQQS+SG S   SS+HN
Sbjct: 1086 GGECASDNLTWLDFLEPELNADNCDISTARDSENNVEVFISDDSQQSVSGLSMPVSSIHN 1145

Query: 541  EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600
            EP+Q+PDFSS+N WDDE+PLQVQASEVLLLPYKEESS+ EV KEDDQASSSILGCAQ+D 
Sbjct: 1146 EPNQRPDFSSVNSWDDEAPLQVQASEVLLLPYKEESSSAEVVKEDDQASSSILGCAQEDF 1205

Query: 601  DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660
            DGFGLGDMFDEPEI +GP+ RPST NEV+ESSF SESDP+EVDN+D+PVSVESCLT+FTK
Sbjct: 1206 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 1265

Query: 661  PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720
            PELLSNENGYNCEKC KRLQQQRLEMKKQSKVA + V NGCQTAVGGDIS  NENSSVEV
Sbjct: 1266 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNENSSVEV 1325

Query: 721  KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780
            KNQRN +L NGS+ Y+SGES NL +N DCSSQ CTKP +CQ GK + LVLDE+EAKVD D
Sbjct: 1326 KNQRNTNLTNGSIIYNSGESSNLKKNADCSSQDCTKPVNCQIGKTDPLVLDEDEAKVDKD 1385

Query: 781  VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840
            +N G + SSGCSNT N+ENSDD+SS SL N++      AK+ IE LSS +SV N + E+S
Sbjct: 1386 MNPGLSCSSGCSNTCNQENSDDKSS-SLPNDEH-----AKTKIEHLSSHLSVGN-QSEES 1445

Query: 841  EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900
            EDGEMDSD T+VNVKRDATKRFLIHKAPPILTIH+KRF+PDARGRYSKLNGHVRFKETID
Sbjct: 1446 EDGEMDSDPTVVNVKRDATKRFLIHKAPPILTIHMKRFTPDARGRYSKLNGHVRFKETID 1505

Query: 901  LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960
            LKPY+DTRC DRDKC+YRL+GVVEHSGSMRGGHYVAYVRGG+R+RSSGEAEEDASVWYYA
Sbjct: 1506 LKPYVDTRCADRDKCNYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYA 1565

Query: 961  SDACVNEVTLDRVLCCEAYILFYEIT 986
            SDA VNEVTLDRVL CEAYILFYEIT
Sbjct: 1566 SDAVVNEVTLDRVLGCEAYILFYEIT 1584

BLAST of MC05g0186 vs. ExPASy TrEMBL
Match: A0A1S4DSI6 (Ubiquitinyl hydrolase 1 OS=Cucumis melo OX=3656 GN=LOC103483482 PE=3 SV=1)

HSP 1 Score: 1573 bits (4073), Expect = 0.0
Identity = 807/986 (81.85%), Postives = 892/986 (90.47%), Query Frame = 0

Query: 1    MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
            MGKKVKKK NRAPPKEKRVSS SP  VPQ S+TTVE A ++ S+VKEK +CRHLDKC NL
Sbjct: 709  MGKKVKKKTNRAPPKEKRVSSNSPRKVPQPSNTTVETADEEVSIVKEKSQCRHLDKCFNL 768

Query: 61   SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
            +ELSSK+GS+EPI+CEDCQ+SSADRRGS+GKA+HGKKKGG  VDVK+D+KAIWICLQCGH
Sbjct: 769  NELSSKLGSAEPISCEDCQDSSADRRGSRGKARHGKKKGGTSVDVKTDAKAIWICLQCGH 828

Query: 121  YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
            YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTL+PVEKTEEN +QKDG
Sbjct: 829  YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLLPVEKTEENGEQKDG 888

Query: 181  LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240
            LSNVVKLIRDRSME +PVDVENT  AS+EVTAATKSESS+L DLDRRN YVVKGLINLGN
Sbjct: 889  LSNVVKLIRDRSMESTPVDVENTRYASNEVTAATKSESSVLIDLDRRNQYVVKGLINLGN 948

Query: 241  TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300
            TCFFNSILQNLLAIDMLRD+F+KLEEC+GPLTIALKK+FIE R E ++KGSINPRSVFGC
Sbjct: 949  TCFFNSILQNLLAIDMLRDHFVKLEECIGPLTIALKKIFIEARTESRMKGSINPRSVFGC 1008

Query: 301  ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360
            ISSKAPQFKGY+QHDSHELLRVLLD LS+EEL SRKMT SKEE+ SGN TPTFVDEMFGG
Sbjct: 1009 ISSKAPQFKGYEQHDSHELLRVLLDALSSEELTSRKMTNSKEERISGNLTPTFVDEMFGG 1068

Query: 361  QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420
            QISSAVCC ECGHTSTVYEPFLDLSLPVP+KKPLAKKVQPVSRAKKTKVPPKR+GKT PK
Sbjct: 1069 QISSAVCCKECGHTSTVYEPFLDLSLPVPMKKPLAKKVQPVSRAKKTKVPPKRNGKTIPK 1128

Query: 421  TEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKEVLIEN 480
            T K SD+V VQIASVPSSS+ESSLP+EA +  T+TIMEKT +SQNVSD K+SEKE+ +EN
Sbjct: 1129 TNKVSDIVPVQIASVPSSSNESSLPSEACASSTTTIMEKTSTSQNVSDAKKSEKEISVEN 1188

Query: 481  GGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLHN 540
            GGEC SD+LTWLDFL PE NA+NC+ ST +DSENNVEVFI+DDSQQS+SG S   SS+HN
Sbjct: 1189 GGECASDNLTWLDFLEPELNADNCDISTARDSENNVEVFISDDSQQSVSGLSMPVSSIHN 1248

Query: 541  EPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDDL 600
            EP+Q+PDFSS+N WDDE+PLQVQASEVLLLPYKEESS+ EV KEDDQASSSILGCAQ+D 
Sbjct: 1249 EPNQRPDFSSVNSWDDEAPLQVQASEVLLLPYKEESSSAEVVKEDDQASSSILGCAQEDF 1308

Query: 601  DGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFTK 660
            DGFGLGDMFDEPEI +GP+ RPST NEV+ESSF SESDP+EVDN+D+PVSVESCLT+FTK
Sbjct: 1309 DGFGLGDMFDEPEIPIGPIGRPSTSNEVAESSFNSESDPEEVDNTDAPVSVESCLTFFTK 1368

Query: 661  PELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVEV 720
            PELLSNENGYNCEKC KRLQQQRLEMKKQSKVA + V NGCQTAVGGDIS  NENSSVEV
Sbjct: 1369 PELLSNENGYNCEKCSKRLQQQRLEMKKQSKVACKAVANGCQTAVGGDISSCNENSSVEV 1428

Query: 721  KNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGD 780
            KNQRN +L NGS+ Y+SGES NL +N DCSSQ CTKP +CQ GK + LVLDE+EAKVD D
Sbjct: 1429 KNQRNTNLTNGSIIYNSGESSNLKKNADCSSQDCTKPVNCQIGKTDPLVLDEDEAKVDKD 1488

Query: 781  VNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEKS 840
            +N G + SSGCSNT N+ENSDD+SS SL N++      AK+ IE LSS +SV N + E+S
Sbjct: 1489 MNPGLSCSSGCSNTCNQENSDDKSS-SLPNDEH-----AKTKIEHLSSHLSVGN-QSEES 1548

Query: 841  EDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETID 900
            EDGEMDSD T+VNVKRDATKRFLIHKAPPILTIH+KRF+PDARGRYSKLNGHVRFKETID
Sbjct: 1549 EDGEMDSDPTVVNVKRDATKRFLIHKAPPILTIHMKRFTPDARGRYSKLNGHVRFKETID 1608

Query: 901  LKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYYA 960
            LKPY+DTRC DRDKC+YRL+GVVEHSGSMRGGHYVAYVRGG+R+RSSGEAEEDASVWYYA
Sbjct: 1609 LKPYVDTRCADRDKCNYRLVGVVEHSGSMRGGHYVAYVRGGNRKRSSGEAEEDASVWYYA 1668

Query: 961  SDACVNEVTLDRVLCCEAYILFYEIT 986
            SDA VNEVTLDRVL CEAYILFYEIT
Sbjct: 1669 SDAVVNEVTLDRVLGCEAYILFYEIT 1687

BLAST of MC05g0186 vs. ExPASy TrEMBL
Match: A0A6J1E9V1 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111432028 PE=3 SV=1)

HSP 1 Score: 1569 bits (4063), Expect = 0.0
Identity = 812/987 (82.27%), Postives = 893/987 (90.48%), Query Frame = 0

Query: 1   MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
           MGKKVKKKNNRAP KEKRVSS SP  VPQ+SS TVEIA +D SVVKE+ +C+HLDKC N 
Sbjct: 1   MGKKVKKKNNRAPLKEKRVSSSSPRKVPQRSSPTVEIAVEDVSVVKERSQCQHLDKCFNF 60

Query: 61  SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
            ELSSK+ SSEPI+CEDCQ+SSADRRGSKGKAKHGKKKGG  VDVK D+KAIWICLQCGH
Sbjct: 61  KELSSKLASSEPISCEDCQDSSADRRGSKGKAKHGKKKGGTSVDVKPDAKAIWICLQCGH 120

Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
           YACGGIGLPTNSQ HAVRHVRQTRHPVVIQFENPQLRWCFSCN L+PVEKT+EN +Q DG
Sbjct: 121 YACGGIGLPTNSQIHAVRHVRQTRHPVVIQFENPQLRWCFSCNKLLPVEKTDENGEQNDG 180

Query: 181 LSNVVKLIRDRSMEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLGN 240
           LSNVVKLIRDR ++ SPVD+ENTW ASSEVTAATKSE S+LSDL+ RNHYVVKGLINLGN
Sbjct: 181 LSNVVKLIRDRLIKASPVDIENTWHASSEVTAATKSECSMLSDLEGRNHYVVKGLINLGN 240

Query: 241 TCFFNSILQNLLAIDMLRDYFLKLEECVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGC 300
           TCFFNSILQNLLAIDMLRD+F+KLEECVGPLTIALKK+FIE RMEG++KGSINPRSVFGC
Sbjct: 241 TCFFNSILQNLLAIDMLRDHFMKLEECVGPLTIALKKIFIEARMEGRMKGSINPRSVFGC 300

Query: 301 ISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTPTFVDEMFGG 360
           ISSKAPQFKGY+QHDSHELL  LLDGLSTEEL SRKMTKSKEE+ SGN TP+FVDEMFGG
Sbjct: 301 ISSKAPQFKGYEQHDSHELLHALLDGLSTEELTSRKMTKSKEERMSGNPTPSFVDEMFGG 360

Query: 361 QISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSGKTFPK 420
           +ISSAVCC ECGHTSTVYEPFL+LSLPVP+KKPLAKKVQPVSRAKKTKVPPK SGKT  K
Sbjct: 361 EISSAVCCKECGHTSTVYEPFLNLSLPVPMKKPLAKKVQPVSRAKKTKVPPKSSGKTIRK 420

Query: 421 TEKASDVVSVQIASVPSSSSESSLPAEASSIG-TSTIMEKTLSSQNVSDVKESEKEVLIE 480
           T K SDVV VQIAS+PSSS+E SL +EAS+   T+TI EKTLSSQNVSDVKE EKEVLIE
Sbjct: 421 TGKPSDVVPVQIASLPSSSNELSLASEASAASSTTTITEKTLSSQNVSDVKEPEKEVLIE 480

Query: 481 NGGECTSDSLTWLDFLGPEPNAENCNTSTTQDSENNVEVFINDDSQQSISGSSNQGSSLH 540
           NGGEC SD+LTW+DFLGPE   ENC+ STTQ+SENNVEVFI+DDSQ SISGS  Q S+LH
Sbjct: 481 NGGECASDNLTWMDFLGPELTVENCDISTTQESENNVEVFISDDSQ-SISGSGMQVSTLH 540

Query: 541 NEPSQQPDFSSINLWDDESPLQVQASEVLLLPYKEESSTTEVAKEDDQASSSILGCAQDD 600
           NEP+Q PDFS +N WD ESPLQVQASEVLLLPYKEESST EVAK DDQASSSILGCAQDD
Sbjct: 541 NEPNQGPDFS-VNSWD-ESPLQVQASEVLLLPYKEESSTAEVAKADDQASSSILGCAQDD 600

Query: 601 LDGFGLGDMFDEPEITVGPLPRPSTVNEVSESSFISESDPDEVDNSDSPVSVESCLTYFT 660
            +GFGLGDMFDEPEI +GP+ RPS  NEV+ESSF+SES+P+EVDN+++PVSVESCLTYFT
Sbjct: 601 FEGFGLGDMFDEPEIPIGPIARPSAANEVAESSFVSESEPEEVDNTNAPVSVESCLTYFT 660

Query: 661 KPELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGGDISCLNENSSVE 720
           KPELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAR+ V NG QT VGGDIS  N +SSVE
Sbjct: 661 KPELLSNENGYNCEKCLKRLQQQRLEMKKQSKVARKAVANGYQTDVGGDISSFNVDSSVE 720

Query: 721 VKNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDG 780
           VKNQRN++L+NGS+ +SSGES+NL EN DCSS+  TKP DCQ GK++SLVL+E+EAKVD 
Sbjct: 721 VKNQRNMNLINGSLSHSSGESLNLKENADCSSRDRTKPVDCQKGKMDSLVLNEDEAKVDK 780

Query: 781 DVNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLSSQISVENSELEK 840
           D+N G AY+SGC+NTSN+ENSDD+S   LSN+D +G GLAK+NIEPLSSQIS EN + EK
Sbjct: 781 DMNPGLAYASGCNNTSNQENSDDKSRSYLSNDDPKGRGLAKTNIEPLSSQISAEN-QPEK 840

Query: 841 SEDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYSKLNGHVRFKETI 900
           SED EM+SDSTIVNVKRDATKRFLIHKAPPILT+HIKRFSPDARGRYSKLNGHV+FKETI
Sbjct: 841 SEDCEMNSDSTIVNVKRDATKRFLIHKAPPILTVHIKRFSPDARGRYSKLNGHVKFKETI 900

Query: 901 DLKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSSGEAEEDASVWYY 960
           DLKPYMDTRC DRDKC YRL+GVVEHSGSMRGGHYVAYVRGG+R+R +GEAEEDASVWYY
Sbjct: 901 DLKPYMDTRCADRDKCKYRLVGVVEHSGSMRGGHYVAYVRGGNRKRRNGEAEEDASVWYY 960

Query: 961 ASDACVNEVTLDRVLCCEAYILFYEIT 986
           ASDACVNEV+LDRVL CEAYILFYEIT
Sbjct: 961 ASDACVNEVSLDRVLGCEAYILFYEIT 983

BLAST of MC05g0186 vs. TAIR 10
Match: AT1G04860.1 (ubiquitin-specific protease 2 )

HSP 1 Score: 653.3 bits (1684), Expect = 3.1e-187
Identity = 438/1057 (41.44%), Postives = 579/1057 (54.78%), Query Frame = 0

Query: 1   MGKKVKKKNNRAPPKEKRVSSISPSTVPQQSSTTVEIAGDDASVVKEKRECRHLDKCLNL 60
           MGKK KKK  RAP KE +   IS     +  S   EIA  D   VKE + C H DK LNL
Sbjct: 1   MGKKAKKK-ARAPTKEIQTMEISKKVSEEPPSQAGEIAEGDVKAVKETQACVHFDKALNL 60

Query: 61  SELSSKIGSSEPINCEDCQESSADRRGSKGKAKHGKKKGGAPVDVKSDSKAIWICLQCGH 120
            ++  KI SS  I C +C E    +RG+K K   GKK   +  D KS++KAIW+CL+CG 
Sbjct: 61  EKVLDKIKSSRQIKCAECNEGVYGKRGTKAKGSKGKKDFSSS-DPKSNNKAIWLCLECGC 120

Query: 121 YACGGIGLPTNSQSHAVRHVRQTRHPVVIQFENPQLRWCFSCNTLIPVEKTEENADQKDG 180
           Y CGG+GLP   QSH +RH R TRH +VIQ+ENPQLRWCF C  L+PVEK E+N ++KD 
Sbjct: 121 YVCGGVGLPNGPQSHVLRHSRVTRHRLVIQWENPQLRWCFPCQLLLPVEK-EDNGEKKDV 180

Query: 181 LSNVVKLIRDRSMEG-SPVDVENTWRASSEVTAATKSESSILSDLDRRNHYVVKGLINLG 240
           LS VVKLI+ RS+   +  D+E+    S  +T+  K E ++ SD++ R+ YVV+GL+NLG
Sbjct: 181 LSEVVKLIKGRSLNNLASSDIEDQCSGSGSITSDIKLEGAVTSDIEARDGYVVRGLVNLG 240

Query: 241 NTCFFNSILQNLLAIDMLRDYFLKL--EECVGPLTIALKKLFIEVRMEGKIKGSINPRSV 300
           NTCFFNSI+QNLL++D LRD+FLK       GPL  +L+KLF E + E  +K  INPR+ 
Sbjct: 241 NTCFFNSIMQNLLSLDRLRDHFLKENGSGVGGPLASSLRKLFTETKPEAGLKSVINPRAF 300

Query: 301 FGCISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKM--TKSKEEKTSGNSTPTFVD 360
           FG   SKAPQF+GYDQHDSHELLR LLD LSTEE A RK       +EK++     T ++
Sbjct: 301 FGSFCSKAPQFRGYDQHDSHELLRCLLDSLSTEESALRKKRGVSDNDEKST-----TLIE 360

Query: 361 EMFGGQISSAVCCIECGHTSTVYEPFLDLSLPVPIKKPLAKKVQPVSRAKKTKVPPKRSG 420
            +FGG+ SS V C+ECGH+S VYEPFLDLSLPVP KK   KK QPVSRAKK K+PPKR  
Sbjct: 361 SVFGGETSSIVSCMECGHSSKVYEPFLDLSLPVPFKKSPPKKPQPVSRAKKAKLPPKRVP 420

Query: 421 KTFPKTEKASDVVSVQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSDVKESEKE 480
           K   K  K S V+   + S  +SS +S      S    S     +L+  +   V E+   
Sbjct: 421 KNVSKVSKVSKVLPGMVLSELNSSGKSMAVTADSDTSCS-----SLAPLDNGPVLETPSV 480

Query: 481 VLIEN--GGECTSDSLT-----WLDFLGPEPNAENCNTSTTQDS---------------- 540
           + ++N    E  S S T     WLDF+GPE + +  N    +D                 
Sbjct: 481 LTLDNNQASESASQSDTGFDGSWLDFIGPETSGDETNLDMQEDGIDNVITAEVNQIVPSP 540

Query: 541 ---------------ENNVEVFIND------------------------DSQQSISGSSN 600
                          E N E  + D                         +   IS   +
Sbjct: 541 NIVANSSVSSGDQTLEGNTERLMQDYEEIAKAEANLDEKDVQAMQSDECPATSGISAEFS 600

Query: 601 QGSSLHNEPSQQPDFSSINLWDDES-PLQVQASEVLLLPYKEESSTTEVAKEDDQASSSI 660
           Q S +  +P      SS+N WD+E  PL V  S++L +PYKE S   +  + + +ASSS 
Sbjct: 601 QASCIGCDPGIGESSSSVNPWDEEELPLVVADSQILYMPYKEISCNDKSVEGECEASSSF 660

Query: 661 LGCAQD--DLDGFGLGDMFDEPEITVGPL---PRPSTVNEVSESSFISESDPDEVDNSDS 720
           +    +  + D    G +FDEPE T GP+   P  +  + V   +F SESDP+E+D+SD 
Sbjct: 661 VTGDHEPQNSDFVDFGGLFDEPETTEGPVFGPPSKAEASGVGFMAFSSESDPEEIDDSDL 720

Query: 721 PVSVESCLTYFTKPELLSNENGYNCEKCLKRLQQQRLEMKKQSKVAREVVVNGCQTAVGG 780
           PVSVE CL +FTK E+LS++N +NCE C K L+ QRL  K++S                 
Sbjct: 721 PVSVERCLGHFTKHEILSDDNAWNCENCSKNLKLQRLREKRKS----------------- 780

Query: 781 DISCLNENSSVEVKNQRNVSLVNGSMCYSSGESMNLHENVDCSSQVCTKPADCQTGKLNS 840
                NE+ S      R+ +  NG                                    
Sbjct: 781 -----NEDES------RSSNTSNG------------------------------------ 840

Query: 841 LVLDENEAKVDGDVNSGQAYSSGCSNTSNRENSDDRSSYSLSNNDSRGSGLAKSNIEPLS 900
             + ENE +  G+              + +++ +D S     ++D R +           
Sbjct: 841 -WVKENEDEGFGETEI----------LAVKQDPNDTSCVKDHSSDGRKAARIH------- 900

Query: 901 SQISVENSELEKSEDGEMDSDSTIVNVKRDATKRFLIHKAPPILTIHIKRFSPDARGRYS 960
              S + SE + ++D + DS+  ++ VKRDATK+ LI+KAPP+LTIH+KRFS D RGR S
Sbjct: 901 ---SADESESKGTQDEDEDSEK-VITVKRDATKKVLINKAPPVLTIHLKRFSQDLRGRLS 955

Query: 961 KLNGHVRFKETIDLKPYMDTRCEDRDKCSYRLIGVVEHSGSMRGGHYVAYVRGGSRRRSS 985
           KLNGHV FKE IDL+ YMD+RC   D   YRL G+VEHSG+MRGGHYVAYVRGG R +  
Sbjct: 961 KLNGHVAFKEVIDLRQYMDSRCSGEDPPVYRLAGLVEHSGTMRGGHYVAYVRGGQRVK-- 955

BLAST of MC05g0186 vs. TAIR 10
Match: AT2G32780.1 (ubiquitin-specific protease 1 )

HSP 1 Score: 490.7 bits (1262), Expect = 2.7e-138
Identity = 398/1145 (34.76%), Postives = 552/1145 (48.21%), Query Frame = 0

Query: 29   QQSSTTVEIAGDDASVVKEKRECRHLDKCLNLSELSSKIGSSEPINCEDCQESSADRRGS 88
            +QS  +VE+  + A  + EKR C H DK ++L +L   I S + I C +C E    +RGS
Sbjct: 10   KQSEQSVEVTEEFAVTMTEKRSCVHFDKYVDLDKLLKIIKSYQQIKCGECNERVHVKRGS 69

Query: 89   KGKAKHGKKKGGAPVDVKSDSKAIWICLQCGHYACGGIGLPTNSQSHAVRHVRQTRHPVV 148
            +  A + ++   +  D      AIW+CL+CG+Y CG +GLPT +QSH + H R TRH +V
Sbjct: 70   RWGASN-RRDWYSSSDQNCARNAIWLCLECGYYVCGDVGLPTEAQSHVMGHNRLTRHRLV 129

Query: 149  IQFENPQLRWCFSCNTLIPVEKTEENADQKDGLSNVVKLIRDRSMEGSPVDVENTWRASS 208
            IQ +NPQLRWCFSC +L+P +  EEN ++KD L  VVKLIR+RS         NT+ AS 
Sbjct: 130  IQCKNPQLRWCFSCQSLLPFD-NEENGEKKDLLLEVVKLIRERS--------PNTYSASF 189

Query: 209  EVTAATKSESSILSDLDRRNHYVVKGLINLGNTCFFNSILQNLLAIDMLRDYFLKLEECV 268
            E   +     SI   ++ R+ Y V+GL+NLGNTCFFNS++QNLL++D LR++FLK +  V
Sbjct: 190  ETEFS--CSGSIYGGIEARDGYAVRGLVNLGNTCFFNSVMQNLLSLDQLREHFLKEDLSV 249

Query: 269  -GPLTIALKKLFIEVRMEGKI-KGSINPRSVFGCISSKAPQFKGYDQHDSHELLRVLLDG 328
             GPL  +LK+LF E   E  + +  INPR +F  + S+APQF+GY QHDSHELLR LLDG
Sbjct: 250  SGPLVSSLKELFAESNSEASVFRNEINPRDLFFSVCSQAPQFRGYQQHDSHELLRCLLDG 309

Query: 329  LSTEELASR-KMTKSKEEKTSGNSTPTFVDEMFGGQISSAVCCIECGHTSTVYEPFLDLS 388
            LS EE + R K+  S    +S    PT +D +FGG+ISS V C+ECGH S VYEPF+DLS
Sbjct: 310  LSIEESSLRKKLGVSDSNDSSTYQKPTLIDSVFGGEISSTVSCLECGHFSKVYEPFMDLS 369

Query: 389  LPVPIKKPLAKKVQPVSRAKK-----------------------TKVP------------ 448
            LPVP  K   KK Q +S+AK+                       + VP            
Sbjct: 370  LPVPSMKLPPKKQQILSQAKEVLKNGAVSKDSEVVSAKPASDHNSTVPLFPSDHKIQSRP 429

Query: 449  -----------------------------------------PKRSGKTFPKTEKASDVVS 508
                                                       R+GKT P  E   DV +
Sbjct: 430  ETSDNETDLVLLSDVSDTAPSTEAKGVNQILVGSTETLMHDNDRTGKTVPDKE---DVRA 489

Query: 509  VQIASVPSSSSESSLPAEASSIGTSTIMEKTLSSQNVSD----VKESEKEVL-------- 568
             Q     S+S  S++  EA   G   + + + S+   +D    +  ++ +VL        
Sbjct: 490  TQSNEETSASGISAVIDEAQVCGCPDLEQSSSSANQGADEELALMVADSQVLYMPYKDHL 549

Query: 569  --------------IENGGECTSDSLTWLDFLGPEPNAENCNT----STTQDSENNVEVF 628
                          +    E   D   +  FL  EP           S ++  E   +  
Sbjct: 550  FYDDYMVAEASSSFVSGDHEPKKDYFDFSSFL-DEPEIREGPVFRPLSKSEVYEAGFKAD 609

Query: 629  INDDSQQSISGSSNQGSSLHNEPSQQPDFSSINLWDDESPLQ-------VQASEVLLLPY 688
             +DD   S        S + ++  Q  D+   + + DE  +        +  SEV    +
Sbjct: 610  CSDDKTVSAGKGEASSSFISSDHEQNIDYVDFSSFFDEPEISERPFFRPLSKSEVSEAGF 669

Query: 689  KEESS--TTEVAKEDDQASSSILGCAQDDLDGFGLGDMFDEPEITVGPLPRPSTVNEVSE 748
            K + S   T  A + + +SS +    + ++D       FDEPEI+  P  RP + +EVSE
Sbjct: 670  KADCSDDKTVSAGKGEASSSFVSSDHEQNIDYVDFSSFFDEPEISERPFFRPLSKSEVSE 729

Query: 749  SSFI-------------------------------------------------------- 808
            + F+                                                        
Sbjct: 730  AGFMAVSGNDKTVRAGKGETFSSFMSGDNERNIDYVEFTNRIFDDRGTSERPVFGPPSKA 789

Query: 809  -----------SESDPDEVDNSDSPVSVESCLTYFTKPELLSNENGYNCEKCLKRLQQQR 868
                       S+SDP  +D SDSPVSV+ CL  FTK E+LS +N ++CE C K L+ QR
Sbjct: 790  KVSEAGFVAVSSDSDPAVLDESDSPVSVDRCLAQFTKHEILSEDNAWHCENCSKNLKLQR 849

Query: 869  LEMKKQSKVA---REVVVNGCQTAVGGDISCLNENSSVEVKNQRNVSLVNGSMCYSSGES 928
            L  K+++K     R V  NG  +A      C   +SS+   NQ  + L NG   Y +   
Sbjct: 850  LREKRRTKEGLSNRWVNENGASSAFD---EC--RDSSL---NQSCIDLENG---YKAAPP 909

Query: 929  MNLHENVDCSSQVCTKPADCQTGKLNSLVLDENEAKVDGDVNSGQAYSSGCSNTSNRENS 985
            +             TK  +C+         +E  A  DG V                EN+
Sbjct: 910  I-------------TKLPNCK---------EEESAIDDGFV--------------GEENT 969

BLAST of MC05g0186 vs. TAIR 10
Match: AT4G10590.2 (ubiquitin-specific protease 10 )

HSP 1 Score: 87.8 bits (216), Expect = 5.3e-17
Identity = 69/199 (34.67%), Postives = 95/199 (47.74%), Query Frame = 0

Query: 218 SSILSDLDRRNHYVVKGLINLGNTCFFNSILQNLLAIDMLRDYFLK-----------LEE 277
           S+ LS L +     + GL NLGNTCF NS LQ L     + +YFL+           L  
Sbjct: 288 SNSLSILGKGEKGGLAGLSNLGNTCFMNSALQCLAHTPPIVEYFLQDYSDDINRDNPLGM 347

Query: 278 CVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGCISSKAPQFKGYDQHDSHELLRVLLDG 337
           C G L IA   L  ++   G  + S+ PR+    ++  APQF GY+QHDS ELL  LLDG
Sbjct: 348 C-GELAIAFGDLLKKLWSSG--RNSVAPRAFKTKLARFAPQFSGYNQHDSQELLAFLLDG 407

Query: 338 LSTEELASRKMTKSKEEKTSGN----------------STPTFVDEMFGGQISSAVCCIE 390
           L  E+L   K     E K S +                   + + ++  GQ  S + C  
Sbjct: 408 LH-EDLNKVKRKPYIELKDSDSRPDDEVAEELWNYHKARNDSVIVDVCQGQYKSTLVCPA 467

BLAST of MC05g0186 vs. TAIR 10
Match: AT4G10590.1 (ubiquitin-specific protease 10 )

HSP 1 Score: 87.8 bits (216), Expect = 5.3e-17
Identity = 69/199 (34.67%), Postives = 95/199 (47.74%), Query Frame = 0

Query: 218 SSILSDLDRRNHYVVKGLINLGNTCFFNSILQNLLAIDMLRDYFLK-----------LEE 277
           S+ LS L +     + GL NLGNTCF NS LQ L     + +YFL+           L  
Sbjct: 288 SNSLSILGKGEKGGLAGLSNLGNTCFMNSALQCLAHTPPIVEYFLQDYSDDINRDNPLGM 347

Query: 278 CVGPLTIALKKLFIEVRMEGKIKGSINPRSVFGCISSKAPQFKGYDQHDSHELLRVLLDG 337
           C G L IA   L  ++   G  + S+ PR+    ++  APQF GY+QHDS ELL  LLDG
Sbjct: 348 C-GELAIAFGDLLKKLWSSG--RNSVAPRAFKTKLARFAPQFSGYNQHDSQELLAFLLDG 407

Query: 338 LSTEELASRKMTKSKEEKTSGN----------------STPTFVDEMFGGQISSAVCCIE 390
           L  E+L   K     E K S +                   + + ++  GQ  S + C  
Sbjct: 408 LH-EDLNKVKRKPYIELKDSDSRPDDEVAEELWNYHKARNDSVIVDVCQGQYKSTLVCPA 467

BLAST of MC05g0186 vs. TAIR 10
Match: AT5G22030.2 (ubiquitin-specific protease 8 )

HSP 1 Score: 87.8 bits (216), Expect = 5.3e-17
Identity = 83/287 (28.92%), Postives = 123/287 (42.86%), Query Frame = 0

Query: 148 VIQFENPQLR-WCFSCNTLIPVEK-------TEENADQK-------DGLSNVVKLIRDRS 207
           +   ++ QLR W  S  T +  E         ++ ADQ+        GLS+ +KL   + 
Sbjct: 218 IFSLDSEQLRIWDISGQTTLFFESDVSNSKDCQQQADQEILLELQIYGLSDSIKLKESKK 277

Query: 208 MEGSPVDVENTWRASSEVTAATKSESSILSDLDRRNH---YVVKGLINLGNTCFFNSILQ 267
            +GS           +  T      S+ LS L +        + GL NLGNTCF NS LQ
Sbjct: 278 EDGSTQQTNGITNGMNGGTVFRFGRSNSLSFLGKAGEAGTLGLTGLQNLGNTCFMNSSLQ 337

Query: 268 NLLAIDMLRDYFL-------KLEECV---GPLTIALKKLFIEVRMEGKIKGSINPRSVFG 327
            L     L D+FL        L+  +   G + +A   L   +   G    ++ PR+   
Sbjct: 338 CLAHTPKLVDFFLGEYSKEINLDNPLGMKGEIALAFGDLLRSLWAPG--ASTVAPRTFKA 397

Query: 328 CISSKAPQFKGYDQHDSHELLRVLLDGLSTEELASRKMTKSKEEKTSGNSTP-------- 387
            ++  APQF G++QHDS ELL  LLDGL   E  +R   K   E   G+  P        
Sbjct: 398 KLARFAPQFSGFNQHDSQELLAFLLDGL--HEDLNRVKNKPYVEAKDGDGRPDAEVADEY 457

Query: 388 ---------TFVDEMFGGQISSAVCCIECGHTSTVYEPFLDLSLPVP 390
                    + + ++  GQ  S + C  C   S +++PF+ LSLP+P
Sbjct: 458 WRNHVARNDSIIVDVCQGQYKSTLVCPICKKVSVMFDPFMYLSLPLP 500