MC05g0185 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0185
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Calcium-binding EF-hand family protein
Location	MC05: 1294215 .. 1296911 (+)
RNA-Seq Expression	MC05g0185
Synteny	MC05g0185

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTAAAAGCACAAGGCATATAGGATTATACTTATGCAGAGCTGCTCTGTTTTCAAAGAAATCCATAATGGCATCTCCACTTTGGACCAGTCCGCTACCCAAATCGTATTTTTCTTCTCTCTTTTGCTTTTCTGTTTCCCACCCGAGAAATTTAGAATACCTCGCTTCTCAGTGTTTCCATGGAAGATTCGCCTGGTGAGTACAAATTTCTTCACAGTTCCACGTTTTTTTCTTTTTCTCGAGGAAGAAAATAGGCTTTTCGTTTTGCAGTTCTTACGCGTAAGAGAAATTAGTCTGTTCGTCGTCTTGGAATCTTCTATTTATTTATTTATTTTTGCGAAAATTTTTAATATAAGTTCCGTGGTTTGCTGTGTGCTGAAATAGAATTCTTCTGGCTCGTTGAATGTTGCTCGTGGTGCCGATGGTGCAATCTAGGGGTTTACTTCGATGTTCAAGCCATTTTTGACTGGTTGCTTTGAATTTACGTTAAATTAAATATATAATTTGCTATGAACATAAGTATCTGGTTGAAACCCCGAACTGAAATTTTATACCAGGGTTGTTCCCAAATGAAAGGGCTGAAGTTTGAGGGTAAGGAAGATGCATAAGGATGGATGACTGAAAATCTGGAAAGGATGCGGTTTTTGCAAGTGATGCAATTTGAACCAAATTAGTTGTCTAGTCTAGAAATTGGAGGAAGATATTGTGGCTGAGGCTTTGGGAAAGGATGGATATCTGGAAATTTGTTTTTGTTGTTAGAATGTACAGATGTAATTCTACTACTTACTAGTTGGAAGAATGAACTTGTTTTTAGTTTTTTTCTCTTTACTTGGCAATACTCTGGAAGAAACTTTGGAGTGTACCTGAAATTCCTTTTCGGGGTTTCAAGTGGATGTAAAGTAAATGATTGATGAAAGAGAGGAAACTACTTCTAAAATGCTTGTATATGTAGCATTTTCTCTTTCATTTTTATAGACAAGAAACGATAAACGTTGCAACTTGTACGGTCTTACAACGAACGGTTCTGTGTATCCCTAATCTCAGGTCATTTTTGGCTTTATGACAACTTCAGGAATTGCTATCTTGCTCATTCATAGGATACACTGATATTGTTTTCATTTGACTTGTCAGGCACACATTGATTTTCTCTATCTTTTATTCTAAAGGAGCATCTCATTCTTGCCCTCGTGTCCAAAGTTGGAAGCATGTTTCTCTGCTGCAGCTCATCAAATAAATATAAGAGATTGGATGCAAAGCTTGGAAGAAAAATGCTTGAAGTAAAAAAAAATTCAACTGGACATGACAATTTCAAGTCACTAAATGGCATAATCTTGAGATTTCCTCAATTCAAAGAGGGGTTGCAAAACATCAGGGGTGTATTTGAGCAGTATGGTGAGCTTTTCTTTTTCGATCTGCGTCACTCTTTCTGAGCTTCTCCTGCTCAAGATTTTCTCACAGTGTCTCTCTCTCTCTCTCTCACAGATGAAGATTCCAATGGAAGCATTGATCGTGCAGAACTCAAAAAATGCTTACAACAGCTGCAAATGCATATGACTGAGGAGGAAGTCGAGGATCTATTCCGCTCTTGTGATATAGATGGTAGCTCTGGAATACAATTTAACGAGTTCATCGTTCTCCTATGTCTCATTTATCTCCTTAAGGACGAGCATTCGCAGACCGTACACACAAACAACCTTAGTCACCCCTCCCATTTCATTCAGTCTTCAACCTGTTTATTATTCTGTTTATCATTCTTGGCATAAGCGTAACCATCCTGAAGCTTTGTTGTTACAGAAATCAAAGCTGGGTTCACCTCAACTTGAAGCAACTTTTGATACAATCATCCAAGCTTTCATATTTCTCGATAAAAACGGCGATGGCAAGCTCAACAAGAAAGAGATGGTCAAGGCTCTGAACGAGGGTTCTCCGAGCGAGAGATCCCCGGCACGCATTACAAAGACAAGATTCAGTATTGCTCCCTTCACTAAAGATACTCCTTCCATTTTTTTTGTCTAGCAAAATCATCCTAGAAAGTTCAAACATGTCGATCTTATCTGGTCAAATAACGTGTTGAATCCTTTTGAACTGTTTTGATTTTTTTATTTTATATTCATTTCCCCCCTGTTTTCTCAATGGATGCAGAAGAAATGGACTGGAACAATAGTGGGAAGGTCAATTTTAGGGAGTTTCTGTTTGGTTTCATTAACTGGGTTGGAATTGGCATGGATGATGATCTCTCTCTTACATGAAGTGTAAACACAGCAGCGGACATCAAGACACCATGGAGACAATGGCTGAAAAAAATAAGGTCAGAATGATATCTCATATTCAAAAATGAAAGACTTGTCTAGTGATTATAGTGAGAGTGAGACTACTCTTGTTTGTTTATTTCATTTGACGAATTGTAATGTTTGGACGACCATGTGAATTAGATTATAGAATGTTATTGTCATCGTCATCGTCATTGTCATCATCATCGTCATTGTTGTTACTGTATATATATCGACTCGTAGAATCTGATCTGGTGAAAGCTAACTGAAGAGAGAGTGGTTAATTTGAAACAACTTCTATAAGCATATGTAAGCCTTCATTTTTTGCTCTTTTTATTGGGTAAGGCAGTTGAATCAGACCCTTTTTTCCCAAAAGAAAAATGTATATGACATTGATTTGGATTGCTTATGAAATGTTCCTGAATTT

mRNA sequence

ATTTAAAAGCACAAGGCATATAGGATTATACTTATGCAGAGCTGCTCTGTTTTCAAAGAAATCCATAATGGCATCTCCACTTTGGACCAGTCCGCTACCCAAATCGTATTTTTCTTCTCTCTTTTGCTTTTCTGTTTCCCACCCGAGAAATTTAGAATACCTCGCTTCTCAGTGTTTCCATGGAAGATTCGCCTGGAGCATCTCATTCTTGCCCTCGTGTCCAAAGTTGGAAGCATGTTTCTCTGCTGCAGCTCATCAAATAAATATAAGAGATTGGATGCAAAGCTTGGAAGAAAAATGCTTGAAGTAAAAAAAAATTCAACTGGACATGACAATTTCAAGTCACTAAATGGCATAATCTTGAGATTTCCTCAATTCAAAGAGGGGTTGCAAAACATCAGGGGTGTATTTGAGCAGTATGATGAAGATTCCAATGGAAGCATTGATCGTGCAGAACTCAAAAAATGCTTACAACAGCTGCAAATGCATATGACTGAGGAGGAAGTCGAGGATCTATTCCGCTCTTGTGATATAGATGGTAGCTCTGGAATACAATTTAACGAGTTCATCGTTCTCCTATGTCTCATTTATCTCCTTAAGGACGAGCATTCGCAGACCAAATCAAAGCTGGGTTCACCTCAACTTGAAGCAACTTTTGATACAATCATCCAAGCTTTCATATTTCTCGATAAAAACGGCGATGGCAAGCTCAACAAGAAAGAGATGGTCAAGGCTCTGAACGAGGGTTCTCCGAGCGAGAGATCCCCGGCACGCATTACAAAGACAAGATTCAAAGAAATGGACTGGAACAATAGTGGGAAGGTCAATTTTAGGGAGTTTCTGTTTGGTTTCATTAACTGGGTTGGAATTGGCATGGATGATGATCTCTCTCTTACATGAAGTGTAAACACAGCAGCGGACATCAAGACACCATGGAGACAATGGCTGAAAAAAATAAGGTCAGAATGATATCTCATATTCAAAAATGAAAGACTTGTCTAGTGATTATAGTGAGAGTGAGACTACTCTTGTTTGTTTATTTCATTTGACGAATTGTAATGTTTGGACGACCATGTGAATTAGATTATAGAATGTTATTGTCATCGTCATCGTCATTGTCATCATCATCGTCATTGTTGTTACTGTATATATATCGACTCGTAGAATCTGATCTGGTGAAAGCTAACTGAAGAGAGAGTGGTTAATTTGAAACAACTTCTATAAGCATATGTAAGCCTTCATTTTTTGCTCTTTTTATTGGGTAAGGCAGTTGAATCAGACCCTTTTTTCCCAAAAGAAAAATGTATATGACATTGATTTGGATTGCTTATGAAATGTTCCTGAATTT

Coding sequence (CDS)

ATGCAGAGCTGCTCTGTTTTCAAAGAAATCCATAATGGCATCTCCACTTTGGACCAGTCCGCTACCCAAATCGTATTTTTCTTCTCTCTTTTGCTTTTCTGTTTCCCACCCGAGAAATTTAGAATACCTCGCTTCTCAGTGTTTCCATGGAAGATTCGCCTGGAGCATCTCATTCTTGCCCTCGTGTCCAAAGTTGGAAGCATGTTTCTCTGCTGCAGCTCATCAAATAAATATAAGAGATTGGATGCAAAGCTTGGAAGAAAAATGCTTGAAGTAAAAAAAAATTCAACTGGACATGACAATTTCAAGTCACTAAATGGCATAATCTTGAGATTTCCTCAATTCAAAGAGGGGTTGCAAAACATCAGGGGTGTATTTGAGCAGTATGATGAAGATTCCAATGGAAGCATTGATCGTGCAGAACTCAAAAAATGCTTACAACAGCTGCAAATGCATATGACTGAGGAGGAAGTCGAGGATCTATTCCGCTCTTGTGATATAGATGGTAGCTCTGGAATACAATTTAACGAGTTCATCGTTCTCCTATGTCTCATTTATCTCCTTAAGGACGAGCATTCGCAGACCAAATCAAAGCTGGGTTCACCTCAACTTGAAGCAACTTTTGATACAATCATCCAAGCTTTCATATTTCTCGATAAAAACGGCGATGGCAAGCTCAACAAGAAAGAGATGGTCAAGGCTCTGAACGAGGGTTCTCCGAGCGAGAGATCCCCGGCACGCATTACAAAGACAAGATTCAAAGAAATGGACTGGAACAATAGTGGGAAGGTCAATTTTAGGGAGTTTCTGTTTGGTTTCATTAACTGGGTTGGAATTGGCATGGATGATGATCTCTCTCTTACATGA

Protein sequence

MQSCSVFKEIHNGISTLDQSATQIVFFFSLLLFCFPPEKFRIPRFSVFPWKIRLEHLILALVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT

Homology

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BLAST of MC05g0185 vs. ExPASy Swiss-Prot
Match: Q9LRN6 (Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.2e-64
Identity = 121/224 (54.02%), Postives = 161/224 (71.88%), Query Frame = 0


Query: 68  MFLCCSSS---NKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRG 127
           M  CC +S    KY  LDAKL RKM+E +    GH + KS++ II++FP+ +EGL+NIR 
Sbjct: 1   MLCCCVNSENNKKYAELDAKLARKMVESRSIYPGHRSLKSMDSIIMKFPKLREGLRNIRS 60

Query: 128 VFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCL 187
           VFE YD D+NG+ID  ELKKCL++L++ +++EEV+ L+  CD+DGS GIQFNEFIVLLCL
Sbjct: 61  VFESYDNDTNGTIDIEELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCL 120

Query: 188 IYLL---KDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKAL-NEGSP 247
           IYLL     E S    ++G   +E+ FD I++ F+FLDK+G GKLNK +++K L NE  P
Sbjct: 121 IYLLAKPSSESSTESREMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYP 180

Query: 248 SERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDD 285
            ERSP+ +T  RF+EMDW   GKV FREFLF F++WVG+   DD
Sbjct: 181 LERSPSHVTNMRFEEMDWGRKGKVGFREFLFAFMSWVGLDDADD 224

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BLAST of MC05g0185 vs. ExPASy Swiss-Prot
Match: Q52K82 (Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 6.9e-55
Identity = 101/206 (49.03%), Postives = 150/206 (72.82%), Query Frame = 0


Query: 82  DAKLGRKMLE-VKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRA 141
           + KL  K++E V++ ++     KS N I+L+FP+  +GL+N + +F+++DEDSNGSID  
Sbjct: 18  ETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHT 77

Query: 142 ELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCLIYLLKDEHS--QTKSK 201
           ELK C+++L++   EEE+ DLF++CDI+   GI F EFIVLLCL+YLLKD+ S  Q K  
Sbjct: 78  ELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWT 137

Query: 202 LGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSERSPARITKTRFKEMDW 261
           +G P+LE TF+T++  F+FLD+N DG ++++EMV+A++E    ERS  RI   RF+EMDW
Sbjct: 138 MGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDES--GERSSGRIAMKRFEEMDW 197

Query: 262 NNSGKVNFREFLFGFINWVGIGMDDD 285
           + +G VNF+EFLF F  WVGI  +++
Sbjct: 198 DKNGMVNFKEFLFAFTQWVGIDENEE 221

[Close]

BLAST of MC05g0185 vs. ExPASy Swiss-Prot
Match: Q9ZSA3 (Calcium-dependent protein kinase 22 OS=Arabidopsis thaliana OX=3702 GN=CPK22 PE=3 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 2.1e-11
Identity = 60/234 (25.64%), Postives = 105/234 (44.87%), Query Frame = 0


Query: 45  FSVFPWKI---RLEHLILALVSK-----VGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNS 104
           F   PW +   + +HLI  +++K     + +  +      K +  D  +   +L   K  
Sbjct: 266 FESQPWPLISFKAKHLIGKMLTKKPKERISAADVLEHPWMKSEAPDKPIDNVVLSRMKQF 325

Query: 105 TGHDNFKSLNGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEE 164
              +  K L   ++     +E ++ ++ +FE  D D +GSI   ELK  L +    ++E 
Sbjct: 326 RAMNKLKKLALKVIAEGLSEEEIKGLKTMFENMDMDKSGSITYEELKMGLNRHGSKLSET 385

Query: 165 EVEDLFRSCDIDGSSGIQFNEFIVLLCLIYLL-KDEHSQTKSKLGSPQLEATFDTIIQAF 224
           EV+ L  + D+DG+  I + EFI      + L +DEH                  + +AF
Sbjct: 386 EVKQLMEAADVDGNGTIDYIEFISATMHRHRLERDEH------------------LYKAF 445

Query: 225 IFLDKNGDGKLNKKEMVKALNEGSPSERSPARITKTRFKEMDWNNSGKVNFREF 270
            + DK+G G + K+E+  A+ E    + + A   K    E D NN GK+++ EF
Sbjct: 446 QYFDKDGSGHITKEEVEIAMKEHGMGDEANA---KDLISEFDKNNDGKIDYEEF 478

[Close]

BLAST of MC05g0185 vs. ExPASy Swiss-Prot
Match: P41210 (Caltractin OS=Atriplex nummularia OX=3553 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 8.0e-11
Identity = 44/151 (29.14%), Postives = 73/151 (48.34%), Query Frame = 0


Query: 120 QNIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFI 179
           Q I+  FE +D D +G+ID  EL   ++ L   MTEE++  +    D DGS  I F+EF 
Sbjct: 25  QEIKEAFELFDTDGSGTIDAKELNVAMRALGFEMTEEQINQMIADVDKDGSGAIDFDEF- 84

Query: 180 VLLCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGS 239
              C +   K     TK +L            ++AF  +D++ +GK++ +++ +   E  
Sbjct: 85  ---CHMMTAKIGERDTKEEL------------MKAFRIIDQDNNGKISPEDIQRIAKE-- 144

Query: 240 PSERSPARITKTRFKEMDWNNSGKVNFREFL 271
             E    +  +   +E D +  G+VN  EFL
Sbjct: 145 LGENFTVKDIQDMIEEADRDRDGEVNVEEFL 157

[Close]

BLAST of MC05g0185 vs. ExPASy Swiss-Prot
Match: P02589 (Troponin C, skeletal muscle OS=Pelophylax lessonae OX=45623 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 8.0e-11
Identity = 46/155 (29.68%), Postives = 76/155 (49.03%), Query Frame = 0


Query: 116 KEGLQNIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQF 175
           +E +   +  F+ +D D  G I   EL   ++ L    T+EE++ +    D DGS  I F
Sbjct: 16  EEMIAEFKAAFDMFDTDGGGDISTKELGTVMRMLGQTPTKEELDAIIEEVDEDGSGTIDF 75

Query: 176 NEFIVLLCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKAL 235
            EF+V+  ++  +K E +Q KS+          + + + F   DKN DG ++ +E+ + L
Sbjct: 76  EEFLVM--MVRQMK-EDAQGKSE----------EELAECFRIFDKNADGYIDSEELGEIL 135

Query: 236 NEGSPSERSPARITKTRFKEMDWNNSGKVNFREFL 271
              S  E       +   K+ D NN GK++F EFL
Sbjct: 136 R--SSGESITDEEIEELMKDGDKNNDGKIDFDEFL 155

[Close]

BLAST of MC05g0185 vs. NCBI nr
Match: XP_022147129.1 (probable calcium-binding protein CML22 isoform X1 [Momordica charantia])

HSP 1 Score: 579 bits (1493), Expect = 1.46e-208
Identity = 287/288 (99.65%), Postives = 288/288 (100.00%), Query Frame = 0


Query: 1   MQSCSVFKEIHNGISTLDQSATQIVFFFSLLLFCFPPEKFRIPRFSVFPWKIRLEHLILA 60
           MQSCSVFKEIHNGISTLDQSATQIVFFFSLLLFCFPPEKFRIPRFSVFPWKIRLEHLILA
Sbjct: 1   MQSCSVFKEIHNGISTLDQSATQIVFFFSLLLFCFPPEKFRIPRFSVFPWKIRLEHLILA 60

Query: 61  LVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQ 120
           LVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQ
Sbjct: 61  LVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQ 120

Query: 121 NIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIV 180
           NIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIV
Sbjct: 121 NIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIV 180

Query: 181 LLCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSP 240
           LLCLIYLLKDEHSQT+SKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSP
Sbjct: 181 LLCLIYLLKDEHSQTESKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSP 240

Query: 241 SERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288
           SERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT
Sbjct: 241 SERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288

[Close]

BLAST of MC05g0185 vs. NCBI nr
Match: XP_022147130.1 (probable calcium-binding protein CML22 isoform X2 [Momordica charantia])

HSP 1 Score: 469 bits (1208), Expect = 1.28e-165
Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 55  EHLILALVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQ 114
           EHLILALVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQ
Sbjct: 24  EHLILALVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQ 83

Query: 115 FKEGLQNIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQ 174
           FKEGLQNIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQ
Sbjct: 84  FKEGLQNIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQ 143

Query: 175 FNEFIVLLCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKA 234
           FNEFIVLLCLIYLLKDEHSQT+SKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKA
Sbjct: 144 FNEFIVLLCLIYLLKDEHSQTESKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKA 203

Query: 235 LNEGSPSERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288
           LNEGSPSERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT
Sbjct: 204 LNEGSPSERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 257

[Close]

BLAST of MC05g0185 vs. NCBI nr
Match: XP_022147132.1 (probable calcium-binding protein CML22 isoform X4 [Momordica charantia] >XP_022147134.1 probable calcium-binding protein CML22 isoform X4 [Momordica charantia])

HSP 1 Score: 446 bits (1147), Expect = 6.80e-157
Identity = 220/221 (99.55%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 68  MFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 127
           MFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRGVFE
Sbjct: 1   MFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60

Query: 128 QYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCLIYL 187
           QYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCLIYL
Sbjct: 61  QYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCLIYL 120

Query: 188 LKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSERSPAR 247
           LKDEHSQT+SKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSERSPAR
Sbjct: 121 LKDEHSQTESKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSERSPAR 180

Query: 248 ITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288
           ITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT
Sbjct: 181 ITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 221

[Close]

BLAST of MC05g0185 vs. NCBI nr
Match: XP_011650876.1 (probable calcium-binding protein CML22 [Cucumis sativus] >XP_031737636.1 probable calcium-binding protein CML22 [Cucumis sativus] >KGN56734.1 hypothetical protein Csa_010293 [Cucumis sativus])

HSP 1 Score: 426 bits (1096), Expect = 4.98e-149
Identity = 209/227 (92.07%), Postives = 216/227 (95.15%), Query Frame = 0


Query: 62  VSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQN 121
           +SKVGS+F CCSSSNKY RLDAKLGRKM+EVK+NS GHDNFKSLNGIILRFPQFKEGLQN
Sbjct: 1   MSKVGSIFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQN 60

Query: 122 IRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVL 181
           IRGVFEQYDEDSNGSIDR ELKKCLQQLQMHMTEEEVEDLF SCDIDGS+GIQFNEFIVL
Sbjct: 61  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVL 120

Query: 182 LCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPS 241
           LCLIYLLKDEHS TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNE SP 
Sbjct: 121 LCLIYLLKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPY 180

Query: 242 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288
           ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVG+  DDDLS T
Sbjct: 181 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLSPT 227

[Close]

BLAST of MC05g0185 vs. NCBI nr
Match: XP_008438356.1 (PREDICTED: probable calcium-binding protein CML22 [Cucumis melo] >XP_008438357.1 PREDICTED: probable calcium-binding protein CML22 [Cucumis melo])

HSP 1 Score: 424 bits (1089), Expect = 5.80e-148
Identity = 207/225 (92.00%), Postives = 215/225 (95.56%), Query Frame = 0


Query: 62  VSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQN 121
           +SKVGS+F CCSSSNKY RLDAKLGRKM+EVK+NS GHDNFKSLNGIILRFPQFKEGLQN
Sbjct: 1   MSKVGSIFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQN 60

Query: 122 IRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVL 181
           IRGVFEQYDEDSNGSIDR ELKKCLQ+LQMHMTEEEVEDLF SCDIDGS+GIQFNEFIVL
Sbjct: 61  IRGVFEQYDEDSNGSIDREELKKCLQKLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVL 120

Query: 182 LCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPS 241
           LCLIYLLKDEHS TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNE SP 
Sbjct: 121 LCLIYLLKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPY 180

Query: 242 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLS 286
           ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVG+  DDDLS
Sbjct: 181 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLS 225

[Close]

BLAST of MC05g0185 vs. ExPASy TrEMBL
Match: A0A6J1D045 (probable calcium-binding protein CML22 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016137 PE=4 SV=1)

HSP 1 Score: 579 bits (1493), Expect = 7.05e-209
Identity = 287/288 (99.65%), Postives = 288/288 (100.00%), Query Frame = 0


Query: 1   MQSCSVFKEIHNGISTLDQSATQIVFFFSLLLFCFPPEKFRIPRFSVFPWKIRLEHLILA 60
           MQSCSVFKEIHNGISTLDQSATQIVFFFSLLLFCFPPEKFRIPRFSVFPWKIRLEHLILA
Sbjct: 1   MQSCSVFKEIHNGISTLDQSATQIVFFFSLLLFCFPPEKFRIPRFSVFPWKIRLEHLILA 60

Query: 61  LVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQ 120
           LVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQ
Sbjct: 61  LVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQ 120

Query: 121 NIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIV 180
           NIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIV
Sbjct: 121 NIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIV 180

Query: 181 LLCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSP 240
           LLCLIYLLKDEHSQT+SKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSP
Sbjct: 181 LLCLIYLLKDEHSQTESKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSP 240

Query: 241 SERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288
           SERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT
Sbjct: 241 SERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288

[Close]

BLAST of MC05g0185 vs. ExPASy TrEMBL
Match: A0A6J1CZA3 (probable calcium-binding protein CML22 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016137 PE=4 SV=1)

HSP 1 Score: 469 bits (1208), Expect = 6.19e-166
Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 55  EHLILALVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQ 114
           EHLILALVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQ
Sbjct: 24  EHLILALVSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQ 83

Query: 115 FKEGLQNIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQ 174
           FKEGLQNIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQ
Sbjct: 84  FKEGLQNIRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQ 143

Query: 175 FNEFIVLLCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKA 234
           FNEFIVLLCLIYLLKDEHSQT+SKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKA
Sbjct: 144 FNEFIVLLCLIYLLKDEHSQTESKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKA 203

Query: 235 LNEGSPSERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288
           LNEGSPSERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT
Sbjct: 204 LNEGSPSERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 257

[Close]

BLAST of MC05g0185 vs. ExPASy TrEMBL
Match: A0A6J1D1I7 (probable calcium-binding protein CML22 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111016137 PE=4 SV=1)

HSP 1 Score: 446 bits (1147), Expect = 3.29e-157
Identity = 220/221 (99.55%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 68  MFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 127
           MFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRGVFE
Sbjct: 1   MFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRGVFE 60

Query: 128 QYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCLIYL 187
           QYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCLIYL
Sbjct: 61  QYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCLIYL 120

Query: 188 LKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSERSPAR 247
           LKDEHSQT+SKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSERSPAR
Sbjct: 121 LKDEHSQTESKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSERSPAR 180

Query: 248 ITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288
           ITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT
Sbjct: 181 ITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 221

[Close]

BLAST of MC05g0185 vs. ExPASy TrEMBL
Match: A0A0A0L6R7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G130890 PE=4 SV=1)

HSP 1 Score: 426 bits (1096), Expect = 2.41e-149
Identity = 209/227 (92.07%), Postives = 216/227 (95.15%), Query Frame = 0


Query: 62  VSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQN 121
           +SKVGS+F CCSSSNKY RLDAKLGRKM+EVK+NS GHDNFKSLNGIILRFPQFKEGLQN
Sbjct: 1   MSKVGSIFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQN 60

Query: 122 IRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVL 181
           IRGVFEQYDEDSNGSIDR ELKKCLQQLQMHMTEEEVEDLF SCDIDGS+GIQFNEFIVL
Sbjct: 61  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVL 120

Query: 182 LCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPS 241
           LCLIYLLKDEHS TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNE SP 
Sbjct: 121 LCLIYLLKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPY 180

Query: 242 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288
           ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVG+  DDDLS T
Sbjct: 181 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLSPT 227

[Close]

BLAST of MC05g0185 vs. ExPASy TrEMBL
Match: A0A1S3AVV2 (probable calcium-binding protein CML22 OS=Cucumis melo OX=3656 GN=LOC103483480 PE=4 SV=1)

HSP 1 Score: 424 bits (1089), Expect = 2.81e-148
Identity = 207/225 (92.00%), Postives = 215/225 (95.56%), Query Frame = 0


Query: 62  VSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQN 121
           +SKVGS+F CCSSSNKY RLDAKLGRKM+EVK+NS GHDNFKSLNGIILRFPQFKEGLQN
Sbjct: 1   MSKVGSIFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQN 60

Query: 122 IRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVL 181
           IRGVFEQYDEDSNGSIDR ELKKCLQ+LQMHMTEEEVEDLF SCDIDGS+GIQFNEFIVL
Sbjct: 61  IRGVFEQYDEDSNGSIDREELKKCLQKLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVL 120

Query: 182 LCLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPS 241
           LCLIYLLKDEHS TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNE SP 
Sbjct: 121 LCLIYLLKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPY 180

Query: 242 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLS 286
           ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVG+  DDDLS
Sbjct: 181 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLS 225

[Close]

BLAST of MC05g0185 vs. TAIR 10
Match: AT3G24110.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 248.1 bits (632), Expect = 8.9e-66
Identity = 121/224 (54.02%), Postives = 161/224 (71.88%), Query Frame = 0


Query: 68  MFLCCSSS---NKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRG 127
           M  CC +S    KY  LDAKL RKM+E +    GH + KS++ II++FP+ +EGL+NIR 
Sbjct: 1   MLCCCVNSENNKKYAELDAKLARKMVESRSIYPGHRSLKSMDSIIMKFPKLREGLRNIRS 60

Query: 128 VFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCL 187
           VFE YD D+NG+ID  ELKKCL++L++ +++EEV+ L+  CD+DGS GIQFNEFIVLLCL
Sbjct: 61  VFESYDNDTNGTIDIEELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCL 120

Query: 188 IYLL---KDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKAL-NEGSP 247
           IYLL     E S    ++G   +E+ FD I++ F+FLDK+G GKLNK +++K L NE  P
Sbjct: 121 IYLLAKPSSESSTESREMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYP 180

Query: 248 SERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDD 285
            ERSP+ +T  RF+EMDW   GKV FREFLF F++WVG+   DD
Sbjct: 181 LERSPSHVTNMRFEEMDWGRKGKVGFREFLFAFMSWVGLDDADD 224

[Close]

BLAST of MC05g0185 vs. TAIR 10
Match: AT4G26470.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 215.7 bits (548), Expect = 4.9e-56
Identity = 101/206 (49.03%), Postives = 150/206 (72.82%), Query Frame = 0


Query: 82  DAKLGRKMLE-VKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRA 141
           + KL  K++E V++ ++     KS N I+L+FP+  +GL+N + +F+++DEDSNGSID  
Sbjct: 18  ETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHT 77

Query: 142 ELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCLIYLLKDEHS--QTKSK 201
           ELK C+++L++   EEE+ DLF++CDI+   GI F EFIVLLCL+YLLKD+ S  Q K  
Sbjct: 78  ELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWT 137

Query: 202 LGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSERSPARITKTRFKEMDW 261
           +G P+LE TF+T++  F+FLD+N DG ++++EMV+A++E    ERS  RI   RF+EMDW
Sbjct: 138 MGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDES--GERSSGRIAMKRFEEMDW 197

Query: 262 NNSGKVNFREFLFGFINWVGIGMDDD 285
           + +G VNF+EFLF F  WVGI  +++
Sbjct: 198 DKNGMVNFKEFLFAFTQWVGIDENEE 221

[Close]

BLAST of MC05g0185 vs. TAIR 10
Match: AT4G26470.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 174.9 bits (442), Expect = 9.6e-44
Identity = 84/175 (48.00%), Postives = 126/175 (72.00%), Query Frame = 0


Query: 82  DAKLGRKMLE-VKKNSTGHDNFKSLNGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRA 141
           + KL  K++E V++ ++     KS N I+L+FP+  +GL+N + +F+++DEDSNGSID  
Sbjct: 18  ETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHT 77

Query: 142 ELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLLCLIYLLKDEHS--QTKSK 201
           ELK C+++L++   EEE+ DLF++CDI+   GI F EFIVLLCL+YLLKD+ S  Q K  
Sbjct: 78  ELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWT 137

Query: 202 LGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSERSPARITKTRF 254
           +G P+LE TF+T++  F+FLD+N DG ++++EMV+A++E    ERS  RI   RF
Sbjct: 138 MGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDES--GERSSGRIAMKRF 190

[Close]

BLAST of MC05g0185 vs. TAIR 10
Match: AT2G41100.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-10
Identity = 39/149 (26.17%), Postives = 71/149 (47.65%), Query Frame = 0


Query: 123 RGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLL 182
           R  F  +D++ +GSI + EL   ++ +    T+ +++DL    D+DG   I F EF+ ++
Sbjct: 14  RESFRLFDKNGDGSITKKELGTMMRSIGEKPTKADLQDLMNEADLDGDGTIDFPEFLCVM 73

Query: 183 CLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSE 242
                       TK  +     +       ++F   DKNGDG + KKE+ + +       
Sbjct: 74  AKNQGHDQAPRHTKKTMADKLTDDQITEYRESFRLFDKNGDGSITKKEL-RTVMFSLGKN 133

Query: 243 RSPARITKTRFKEMDWNNSGKVNFREFLF 272
           R+ A + +    E+D +  G ++F EFL+
Sbjct: 134 RTKADL-QDMMNEVDLDGDGTIDFPEFLY 160

[Close]

BLAST of MC05g0185 vs. TAIR 10
Match: AT2G41100.4 (Calcium-binding EF hand family protein )

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-10
Identity = 39/149 (26.17%), Postives = 71/149 (47.65%), Query Frame = 0


Query: 123 RGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVLL 182
           R  F  +D++ +GSI + EL   ++ +    T+ +++DL    D+DG   I F EF+ ++
Sbjct: 14  RESFRLFDKNGDGSITKKELGTMMRSIGEKPTKADLQDLMNEADLDGDGTIDFPEFLCVM 73

Query: 183 CLIYLLKDEHSQTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPSE 242
                       TK  +     +       ++F   DKNGDG + KKE+ + +       
Sbjct: 74  AKNQGHDQAPRHTKKTMADKLTDDQITEYRESFRLFDKNGDGSITKKEL-RTVMFSLGKN 133

Query: 243 RSPARITKTRFKEMDWNNSGKVNFREFLF 272
           R+ A + +    E+D +  G ++F EFL+
Sbjct: 134 RTKADL-QDMMNEVDLDGDGTIDFPEFLY 160

The following BLAST results are available for this feature:

BLAST of MC05g0185 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LRN6	1.2e-64	54.02	Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 ...	[more]
Q52K82	6.9e-55	49.03	Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 ...	[more]
Q9ZSA3	2.1e-11	25.64	Calcium-dependent protein kinase 22 OS=Arabidopsis thaliana OX=3702 GN=CPK22 PE=...	[more]
P41210	8.0e-11	29.14	Caltractin OS=Atriplex nummularia OX=3553 PE=2 SV=1	[more]
P02589	8.0e-11	29.68	Troponin C, skeletal muscle OS=Pelophylax lessonae OX=45623 PE=1 SV=1	[more]

BLAST of MC05g0185 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147129.1	1.46e-208	99.65	probable calcium-binding protein CML22 isoform X1 [Momordica charantia]	[more]
XP_022147130.1	1.28e-165	99.57	probable calcium-binding protein CML22 isoform X2 [Momordica charantia]	[more]
XP_022147132.1	6.80e-157	99.55	probable calcium-binding protein CML22 isoform X4 [Momordica charantia] >XP_0221...	[more]
XP_011650876.1	4.98e-149	92.07	probable calcium-binding protein CML22 [Cucumis sativus] >XP_031737636.1 probabl...	[more]
XP_008438356.1	5.80e-148	92.00	PREDICTED: probable calcium-binding protein CML22 [Cucumis melo] >XP_008438357.1...	[more]

BLAST of MC05g0185 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D045	7.05e-209	99.65	probable calcium-binding protein CML22 isoform X1 OS=Momordica charantia OX=3673...	[more]
A0A6J1CZA3	6.19e-166	99.57	probable calcium-binding protein CML22 isoform X2 OS=Momordica charantia OX=3673...	[more]
A0A6J1D1I7	3.29e-157	99.55	probable calcium-binding protein CML22 isoform X4 OS=Momordica charantia OX=3673...	[more]
A0A0A0L6R7	2.41e-149	92.07	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G130890 PE=4 SV=1	[more]
A0A1S3AVV2	2.81e-148	92.00	probable calcium-binding protein CML22 OS=Cucumis melo OX=3656 GN=LOC103483480 P...	[more]

BLAST of MC05g0185 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24110.1	8.9e-66	54.02	Calcium-binding EF-hand family protein	[more]
AT4G26470.1	4.9e-56	49.03	Calcium-binding EF-hand family protein	[more]
AT4G26470.2	9.6e-44	48.00	Calcium-binding EF-hand family protein	[more]
AT2G41100.1	1.1e-10	26.17	Calcium-binding EF hand family protein	[more]
AT2G41100.4	1.1e-10	26.17	Calcium-binding EF hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 121..149 e-value: 0.049 score: 22.8 coord: 157..185 e-value: 1.4 score: 16.5 coord: 248..276 e-value: 13.0 score: 8.9 coord: 210..238 e-value: 0.063 score: 22.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 209..270 e-value: 4.8E-8 score: 33.3 coord: 121..180 e-value: 4.0E-11 score: 43.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 153..188 score: 10.189618
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 117..152 score: 12.895472
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 206..241 score: 10.663839
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 244..279 score: 8.320626
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 122..182 e-value: 3.92851E-14 score: 63.7209
None	No IPR available	GENE3D	1.10.238.10		coord: 183..276 e-value: 9.7E-13 score: 49.9
None	No IPR available	GENE3D	1.10.238.10		coord: 82..182 e-value: 5.3E-19 score: 69.9
None	No IPR available	PANTHER	PTHR23064:SF53	CALCIUM-BINDING PROTEIN CML22-RELATED	coord: 59..285
None	No IPR available	PANTHER	PTHR23064	TROPONIN	coord: 59..285
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 130..142
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 219..231
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 116..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0185.1	MC05g0185.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding