MC05g0163 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0163
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDihydroxy-acid dehydratase
LocationMC05: 1134047 .. 1139808 (-)
RNA-Seq ExpressionMC05g0163
SyntenyMC05g0163
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAGTATAAAAGTAACTAATACTCCGATCTCAACTTGTCGTCTACCAAATTTCACTTTCCAATTTCTGCTCTTCTCCTCACTCACTCCCCATCCTTAAACCCTGCTTCCGCCGCCGTCTCTCTCCGGCGCCAGAATGCAATCGGCGATCATTTCACCGGCTCCACGCGCCACCACCTTCATCCCCATTTCACCGCCTGTCTCCACCAATGTGCTCCGGCGGAGCCGTCCCCTCTTTCTCAGAGCCTCGATTTCCAATGTCACCACGCAACCTCCGCCGCCCTCGGTGACCGTAGACTCCCCCCCGCCCAGCTCCTCCGCCGCGCAGAAGCTTAACAAGTATAGCTCGCGCGTCACCGAGCCGAAGTCTCAGGGCGGGTCTCAGGCGATTCTCCACGGCGTCGGCCTCTCCGACGACGATCTCACCAAGCCTCAGATTGGAATTTCGTCGGTATGGTACGAGGGCAATACCTGTAACATGCACTTGTTGAAGCTCTCGGAGGCTGTTAAAGAAGGGGTTAAAGAGGCCGGCATGGTGGGTTTTAGGTTCAATACTATCGGCGTCAGTGACGCCATTTCCATGGGAACCAGAGGCATGTGCTATAGCTTGCAGTCGAGGGATTTGATTGCTGATAGCATTGAGACTGTCATGAGTGCTCAGTGGTACGACGGGAACATCTCCATCCCGGGCTGTGATAAGAATGTGAGCTTTCTCTTAGCTTTTCCCCATTGCTGGGTGTTTCTAGAATTGTATTGAACCTGAGTTCTTTTAGACGGGGCTTTCATTTCTCTGTTTTACTTTCTCTTGTATTTTACAGTACTGCATTTTCATCCCCAACTTACTAGATTCTGCTTGTTAACGCATTTGTTCTCAGGGTTTCTGAAGCTTGTGAAAACTACTGATGCTTGTGTTTCTTTATTAGGTTTTGCTCTTTTGAAATCTTAGTCAAATTTTAAGAACAAGTCTACTTTACTGAACTGGATGTGATAATTTGTGGGTAATAACTTCATTTTTTAGTCTTTCACACTTTCACTTGCATGGTATCACTGCGTAGTTATAATTAGTATTTCTCCATCAAAGCATATGCACTCTGTTCTCTTTAAATCTACAATATTTTGATTGAAAGTTTCGTCTAATACTGCCACACTAATGGTTTTCTGTGAGTCAACAATAGTAATATATATATATATTTTTGGAAAAGGAAACGGAAATATTTTATTATGAAAAGTTACAAAGTTACAAAAGAATCCCGTTAAGAGTTCTACTTGTTTTTTTTTTCCCTCATTACAACTGCGTGCTGATAACTAGCTATTCTACAGATGCCCGGTACAATAATGGCGATGGGTAGGCTGAATCGACCTAGTATTATGGTTTACGGTGGAACGATCAAGGTTTGGGACCTAGATTTTACCCATTTCCATGACTTGAATATAATATTTCAATCGAATCATGTTTGTTAAGAGGCTGGTGTCGTGTTTTCTATCCAAAACCCTGCTAAAAATTATCCTCTTGTAAATGATTTGTATGGAATTTAACACAGAGGAGATGTGTCAATTAATTTCTTGATTCGTTCTGTTTGTATTTGAAAATTTGTAACTTAAAATTTATTTACATGACCATCTTTATCAGAATAGAACTACAGAAGATGATCAATATGAAAGCCTTCACATTTTAGTGAAGTGTGTAACTGAGTTTGTTGCTTTTAGATTTATATGCATTCACTACATGTTGTAGGCATGCCAGTGTGCGTTTATCATGCCCATAATCGTGAGCTATACTCAATCACTCAACTTCCTACCTTCTATAGTTAACCATCAGACACTAGATTTTTGTCCTCATTTGAATGCTATTTAATTAGTGAAACTTTTTGAAGTTGTTTGTAATTTTATCTCTCTTCTGTTGTCTTTTCTTCCGCAGCCTGGTCACTTTCAAGGCCACACATACGACATAGTGTCTGCATTTCAGGTACCTGTGTACTGTGTATTAATGCCAAAAAGTAAGCAAATTTCATTTTCTAAACTTTTGATGTCATTTAAAATTCTCAATATTCATTATTTTAACATATAAAGTTAGTTATGCATTAAAATCTCATTATGTATATGTAAAATTCCTGAATACTTTTTTTGGGGATGATCTCTCTGTGTCTACTTAAACATGTAACTCTGGGAATTAGTAGTACCATAGAAAAATTTGCAGACATTTGATGACCAAAAAAGTTTGGAGTGTGGTCATGTTACATCCTTAAGAAATTCAAGAAAACAAAAAGTAGATCATCTGAAATTGTTTACTCATGATATTGGGTTGTATATGTTCGTTGCTGTGTTTTTTCAAGATTGAAACTTCATCTATTTTAAACTCTTTTATTATTGCGGTTAGGTGTATGGAGAATATGTAAGTGGCTCGATAAGTGACGAACAAAGGAGGAATGTCGTCCGCAACTCCTGTCCTGGGGCTGGGGCTTGTGGTGGAATGTACACAGCTAATACAATGGCCTCTGCTATTGAGGCTATGGGTATGTCTCTTCCTTACAGGTAAGATATGTCCCCAGTCCACAACATTAGTTTGACGTGTAGTTGTGAGGAAAACAGGATTTATCTTATTTCTCATTTGAATCATGTTTCCTGAAGCTCATCAACACCTGCTGAGGATCCATTGAAGTTGGATGAATGCCGTTTAGCTGGAAAATATCTTCTAGAATTATTAAAAATGGATTTAAAACCACGGGATATTATTACAGAAAAATCACTACGAAATGCGATGGTTGTTGTCATGGCTTTGGGTGGCTCTACTAATGCAGTGCTACACTTGATTGCTATAGCAAGGTGAGGAGAGCTTGATGCCAAAATATTCCTTAATTTCTTTCCCCTTTCTGTAGACATCACTATGGCTAATGGCAAGATTCTTGGCAGGTCTGTTGGTTTGGATTTAACCCTTGATGATTTTCAGAAGGTTAGTGATCAGGTTCCTTTCCTTGCTGATCTTAAGCCTAGTGGAAAATATGTCATGGAGGACGTTCACAAGGTACGTATTTTATTTTCATCTTATTTACAGTCATGTAAAATGCTACCACTGGGTGCTTATAGTCTACTGTAGTATGTACCTGTACTATATATATATATATATATGTCCATCCGTCCACCCGTGCTTCTTGATAAGTATCAAGATTTGTTTTGATAGATTGGAGGAACCCCTGGAGTCATTCGCTATTTGTTGGAGAAAGGACTTTTAGATGGAGATTGTATGACTGGTATGTTTTGGTTTTGTACTTCGATGGCGTTTCCAGTTTAACTTCTGTGGTTTCAATTGGTGATGTTCTTTTCCATTTTCTTTTTTAGTCACGGGAAAGACACTCGCGGAAAATGCTAAAATTTTCCTAACTTTATCTGAAGGGCAGGTATGACGGCTTTGATATTATTACATCCAATTTCAAAGTTCAGATTAAAGAAAAGAAAAGGAATCCCCTCCAAAAGTTAACAAGAAACACAACATTAGAGGTGAAAATCTGAAAGAACACTTTATACTTACCTCTTGTACTTTATGCAGGATATTATTAGGCCGTTGGAGAATCCCATTAAGAAAACAGGGCATATTCAAATATTATATGGAAATCTTGCTCCTGAGGGTTCAGTAGCTAAAATTACTGGAAAGGAGGGACTATATTTCTCGGGTTCGTTTTCATGTTCTCAATGAGCTTTCAAGATGCTTGAACAGAAATGTCGTTTACAATTAGATTTTGATATAAAAAGTCGAGTATTCCTCCCCCCTTTTTTAAAAAGAGGAAACAGCTAACTTTTCATTAAACAACTGCGTTGGCCTTGCCTCTTGTAGTGGGAAAATTGACCGTATTACCATTCTGAACCTTATCGTTTATCACAACATACATCCACTGTCCATGTGCCTCTGCATCAGTTCCTACTTCCTTTGCCTGTGGTTGGTAGAGAGTGGATGCTCAAATGAATCAAGAATTCTACTCAAATTCTTGAAGACATTGTCCATAAATCTAGAAAATTTCATCCCTATTTCTGATTTAGAACCAGATTCTTAAAAGAATTTGCAAAGAATGAAAATAAATTTCTATGTAAATACATTATTCTACTTAAAAAAATTTGTTTTGCAGGCCCTGCTCTTGTTTTTGAAGGAGAAGAATCTATGATTGCAGCTATAACAGAGGACCCTTCAAGTTTTAAGGTACTTGATTACATTGGCTAGTTCCTAACTTTTATCTTTGCTAAACTTTTCGTCTTATATTGCTGTTTTGGCCTATCTTTTAGGCCTATAATCTCATCTGATATCTGGATGAAATTATTAGTAACGTGTGTGGATATTTTATGTTAGTTACTAACATTTACAAACAGTAAACTCACTAATTAAACACGGGTATTTATATAACCCAATTATTTACGATATCTATAAATTTAGGCTTCTCATCTCAAAGTTTCATAAATTCAGATTAAAAACTGTAAGCCAAACCCCTTAAAATTTACAGAAACAACCAAAATTTGAAGGGTGCATATGCAGTTGTGTAAGCACGTTTGCATTCACACATATATTCTTTCTGTAGCTTCTAAGACACTCACTTATATTAAGAAAAAGTTGACATGGATAACTTCATTATTTCAGGGTAAAGTAGTGATAATTAGAGGCGAAGGCCCTAAGGGGGGACCGGGAATGCCTGAAATGTTAACACCGACCAGTGCGATAATGGGGGCAGGTCTCGGAAAGGTACGATCGAGTTTAATCAGATTTTCTCTCTGAACCCAGATTACAAAAGTCCCTCTGAAGTAGTTAACCCCATCTTTTGACTGCCATTGACATCCACAGGAAGTGGCATTGCTGACTGATGGTAGGTTCTCTGGAGGTTCACATGGCTTTGTTGTTGGTCACGTATGCCCTGAAGCACAGGTCTGTTTTTGTTCTTGTTTCTCATTTTCATTTCGAAATAGTGCAAGCTTAGAATGATATTGCTGTTCTAGTCCTAGAAATCACTTAAAAAAATTTATAAAATGTTGTTGTAGAAATATAATCTGTATTGCTAGAAACTGTTACGACCGTCCTTTCAACGTACACAAGAAATTTAGAGTTTCGCATGGTTAAGACTGATATCTCTCTATCTCCCTATATACCCACCTCTATGATCAAATAAAGTTCGAGTGCTTTTGTAACCATTTTCCAGGAAGGCGGTCCAATCGGGTTGATTCAAAATGGCGATGTCATCACTGTTGATGTTCAGAATCGGAGAATCGACGTCCAACTATCCGATCAAGAGATGGAAGAACGAAGAAATAACTGGATTCCACCTGCATACAAGGCTACTAGAGGCATCCTCTACAAGGTTTGCCATACCATCTCTATGATTACATATTTGGTTTTTATTTTTCATAAATCGCGTTACTGAAACTACCCACCCTGTTCATCATCCTCAGTACATCAAGAATGTGCAATCGGCATCGAGAGGGTGCGTGACAGACGAGTAGACGAGAGCCTGCAGTTAGAGACCCTTCCTCTAGTTCACATCTTGAGTCAGACAACAAATCCAGTTTCTAAGACACATCCACTCCCTTTCTTGCTTGCTTCACCATTGCTTCACCATTTTGATTCCGAGTCTTTTTCTCCCTTTCCTTTTAGGAAGAAATTTGGCTGTAATTATTGAAGTGGATGAATATTCTATTGTTGTAGTCATTTAAGATGAAGTCTCAATAAAGAGACCATGGAAGAGTCACATAAATTAAAATAAAATTAATGTTCAATTTCTTTTCCCTCCA

mRNA sequence

AACAGTATAAAAGTAACTAATACTCCGATCTCAACTTGTCGTCTACCAAATTTCACTTTCCAATTTCTGCTCTTCTCCTCACTCACTCCCCATCCTTAAACCCTGCTTCCGCCGCCGTCTCTCTCCGGCGCCAGAATGCAATCGGCGATCATTTCACCGGCTCCACGCGCCACCACCTTCATCCCCATTTCACCGCCTGTCTCCACCAATGTGCTCCGGCGGAGCCGTCCCCTCTTTCTCAGAGCCTCGATTTCCAATGTCACCACGCAACCTCCGCCGCCCTCGGTGACCGTAGACTCCCCCCCGCCCAGCTCCTCCGCCGCGCAGAAGCTTAACAAGTATAGCTCGCGCGTCACCGAGCCGAAGTCTCAGGGCGGGTCTCAGGCGATTCTCCACGGCGTCGGCCTCTCCGACGACGATCTCACCAAGCCTCAGATTGGAATTTCGTCGGTATGGTACGAGGGCAATACCTGTAACATGCACTTGTTGAAGCTCTCGGAGGCTGTTAAAGAAGGGGTTAAAGAGGCCGGCATGGTGGGTTTTAGGTTCAATACTATCGGCGTCAGTGACGCCATTTCCATGGGAACCAGAGGCATGTGCTATAGCTTGCAGTCGAGGGATTTGATTGCTGATAGCATTGAGACTGTCATGAGTGCTCAGTGGTACGACGGGAACATCTCCATCCCGGGCTGTGATAAGAATATGCCCGGTACAATAATGGCGATGGGTAGGCTGAATCGACCTAGTATTATGGTTTACGGTGGAACGATCAAGCCTGGTCACTTTCAAGGCCACACATACGACATAGTGTCTGCATTTCAGGTGTATGGAGAATATGTAAGTGGCTCGATAAGTGACGAACAAAGGAGGAATGTCGTCCGCAACTCCTGTCCTGGGGCTGGGGCTTGTGGTGGAATGTACACAGCTAATACAATGGCCTCTGCTATTGAGGCTATGGGTATGTCTCTTCCTTACAGCTCATCAACACCTGCTGAGGATCCATTGAAGTTGGATGAATGCCGTTTAGCTGGAAAATATCTTCTAGAATTATTAAAAATGGATTTAAAACCACGGGATATTATTACAGAAAAATCACTACGAAATGCGATGGTTGTTGTCATGGCTTTGGGTGGCTCTACTAATGCAGTGCTACACTTGATTGCTATAGCAAGGTCTGTTGGTTTGGATTTAACCCTTGATGATTTTCAGAAGGTTAGTGATCAGGTTCCTTTCCTTGCTGATCTTAAGCCTAGTGGAAAATATGTCATGGAGGACGTTCACAAGATTGGAGGAACCCCTGGAGTCATTCGCTATTTGTTGGAGAAAGGACTTTTAGATGGAGATTGTATGACTGTCACGGGAAAGACACTCGCGGAAAATGCTAAAATTTTCCTAACTTTATCTGAAGGGCAGGATATTATTAGGCCGTTGGAGAATCCCATTAAGAAAACAGGGCATATTCAAATATTATATGGAAATCTTGCTCCTGAGGGTTCAGTAGCTAAAATTACTGGAAAGGAGGGACTATATTTCTCGGGCCCTGCTCTTGTTTTTGAAGGAGAAGAATCTATGATTGCAGCTATAACAGAGGACCCTTCAAGTTTTAAGGGTAAAGTAGTGATAATTAGAGGCGAAGGCCCTAAGGGGGGACCGGGAATGCCTGAAATGTTAACACCGACCAGTGCGATAATGGGGGCAGGTCTCGGAAAGGAAGTGGCATTGCTGACTGATGGTAGGTTCTCTGGAGGTTCACATGGCTTTGTTGTTGGTCACGTATGCCCTGAAGCACAGGAAGGCGGTCCAATCGGGTTGATTCAAAATGGCGATGTCATCACTGTTGATGTTCAGAATCGGAGAATCGACGTCCAACTATCCGATCAAGAGATGGAAGAACGAAGAAATAACTGGATTCCACCTGCATACAAGGCTACTAGAGGCATCCTCTACAAGTACATCAAGAATGTGCAATCGGCATCGAGAGGGTGCGTGACAGACGAGTAGACGAGAGCCTGCAGTTAGAGACCCTTCCTCTAGTTCACATCTTGAGTCAGACAACAAATCCAGTTTCTAAGACACATCCACTCCCTTTCTTGCTTGCTTCACCATTGCTTCACCATTTTGATTCCGAGTCTTTTTCTCCCTTTCCTTTTAGGAAGAAATTTGGCTGTAATTATTGAAGTGGATGAATATTCTATTGTTGTAGTCATTTAAGATGAAGTCTCAATAAAGAGACCATGGAAGAGTCACATAAATTAAAATAAAATTAATGTTCAATTTCTTTTCCCTCCA

Coding sequence (CDS)

ATGCAATCGGCGATCATTTCACCGGCTCCACGCGCCACCACCTTCATCCCCATTTCACCGCCTGTCTCCACCAATGTGCTCCGGCGGAGCCGTCCCCTCTTTCTCAGAGCCTCGATTTCCAATGTCACCACGCAACCTCCGCCGCCCTCGGTGACCGTAGACTCCCCCCCGCCCAGCTCCTCCGCCGCGCAGAAGCTTAACAAGTATAGCTCGCGCGTCACCGAGCCGAAGTCTCAGGGCGGGTCTCAGGCGATTCTCCACGGCGTCGGCCTCTCCGACGACGATCTCACCAAGCCTCAGATTGGAATTTCGTCGGTATGGTACGAGGGCAATACCTGTAACATGCACTTGTTGAAGCTCTCGGAGGCTGTTAAAGAAGGGGTTAAAGAGGCCGGCATGGTGGGTTTTAGGTTCAATACTATCGGCGTCAGTGACGCCATTTCCATGGGAACCAGAGGCATGTGCTATAGCTTGCAGTCGAGGGATTTGATTGCTGATAGCATTGAGACTGTCATGAGTGCTCAGTGGTACGACGGGAACATCTCCATCCCGGGCTGTGATAAGAATATGCCCGGTACAATAATGGCGATGGGTAGGCTGAATCGACCTAGTATTATGGTTTACGGTGGAACGATCAAGCCTGGTCACTTTCAAGGCCACACATACGACATAGTGTCTGCATTTCAGGTGTATGGAGAATATGTAAGTGGCTCGATAAGTGACGAACAAAGGAGGAATGTCGTCCGCAACTCCTGTCCTGGGGCTGGGGCTTGTGGTGGAATGTACACAGCTAATACAATGGCCTCTGCTATTGAGGCTATGGGTATGTCTCTTCCTTACAGCTCATCAACACCTGCTGAGGATCCATTGAAGTTGGATGAATGCCGTTTAGCTGGAAAATATCTTCTAGAATTATTAAAAATGGATTTAAAACCACGGGATATTATTACAGAAAAATCACTACGAAATGCGATGGTTGTTGTCATGGCTTTGGGTGGCTCTACTAATGCAGTGCTACACTTGATTGCTATAGCAAGGTCTGTTGGTTTGGATTTAACCCTTGATGATTTTCAGAAGGTTAGTGATCAGGTTCCTTTCCTTGCTGATCTTAAGCCTAGTGGAAAATATGTCATGGAGGACGTTCACAAGATTGGAGGAACCCCTGGAGTCATTCGCTATTTGTTGGAGAAAGGACTTTTAGATGGAGATTGTATGACTGTCACGGGAAAGACACTCGCGGAAAATGCTAAAATTTTCCTAACTTTATCTGAAGGGCAGGATATTATTAGGCCGTTGGAGAATCCCATTAAGAAAACAGGGCATATTCAAATATTATATGGAAATCTTGCTCCTGAGGGTTCAGTAGCTAAAATTACTGGAAAGGAGGGACTATATTTCTCGGGCCCTGCTCTTGTTTTTGAAGGAGAAGAATCTATGATTGCAGCTATAACAGAGGACCCTTCAAGTTTTAAGGGTAAAGTAGTGATAATTAGAGGCGAAGGCCCTAAGGGGGGACCGGGAATGCCTGAAATGTTAACACCGACCAGTGCGATAATGGGGGCAGGTCTCGGAAAGGAAGTGGCATTGCTGACTGATGGTAGGTTCTCTGGAGGTTCACATGGCTTTGTTGTTGGTCACGTATGCCCTGAAGCACAGGAAGGCGGTCCAATCGGGTTGATTCAAAATGGCGATGTCATCACTGTTGATGTTCAGAATCGGAGAATCGACGTCCAACTATCCGATCAAGAGATGGAAGAACGAAGAAATAACTGGATTCCACCTGCATACAAGGCTACTAGAGGCATCCTCTACAAGTACATCAAGAATGTGCAATCGGCATCGAGAGGGTGCGTGACAGACGAGTAG

Protein sequence

MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSSSAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKLSEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGNISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSISDEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGKYLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKVSDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFLTLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMIAAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSHGFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATRGILYKYIKNVQSASRGCVTDE
Homology
BLAST of MC05g0163 vs. ExPASy Swiss-Prot
Match: Q9LIR4 (Dihydroxy-acid dehydratase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DHAD PE=1 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 4.0e-294
Identity = 507/621 (81.64%), Postives = 548/621 (88.24%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQ+ I S  PRAT F P  P + ++ +   RP  +  S  +VT  P PP    +      
Sbjct: 1   MQATIFS--PRATLF-PCKPLLPSHNVNSRRPSIISCSAQSVTADPSPPITDTN------ 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
               KLNKYSSR+TEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  ----KLNKYSSRITEPKSQGGSQAILHGVGLSDDDLLKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVKEGV+ AGMVGFRFNTIGVSDAISMGTRGMC+SLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKEGVENAGMVGFRFNTIGVSDAISMGTRGMCFSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRP IMVYGGTIKPGHFQ  TYDIVSAFQ YGE+VSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPGIMVYGGTIKPGHFQDKTYDIVSAFQSYGEFVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DEQR+ V+ +SCPGAGACGGMYTANTMASAIEAMGMSLPYSSS PAEDPLKLDECRLAGK
Sbjct: 241 DEQRKTVLHHSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSIPAEDPLKLDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIIT KSLRNAMV VMALGGSTNAVLHLIAIARSVGL+LTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITPKSLRNAMVSVMALGGSTNAVLHLIAIARSVGLELTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SD VPFLADLKPSGKYVMED+HKIGGTP V+RYLLE GL+DGDCMTVTG+TLA+N +   
Sbjct: 361 SDAVPFLADLKPSGKYVMEDIHKIGGTPAVLRYLLELGLMDGDCMTVTGQTLAQNLENVP 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
           +L+EGQ+IIRPL NPIK+TGHIQIL G+LAP+GSVAKITGKEGLYFSGPALVFEGEESM+
Sbjct: 421 SLTEGQEIIRPLSNPIKETGHIQILRGDLAPDGSVAKITGKEGLYFSGPALVFEGEESML 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAI+ DP SFKG VV+IRGEGPKGGPGMPEMLTPTSAIMGAGLGKE ALLTDGRFSGGSH
Sbjct: 481 AAISADPMSFKGTVVVIRGEGPKGGPGMPEMLTPTSAIMGAGLGKECALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEAQEGGPIGLI+NGD+IT+D+  +RID Q+S +EM +RR  W  PAYK  R
Sbjct: 541 GFVVGHICPEAQEGGPIGLIKNGDIITIDIGKKRIDTQVSPEEMNDRRKKWTAPAYKVNR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 622
           G+LYKYIKNVQSAS GCVTDE
Sbjct: 601 GVLYKYIKNVQSASDGCVTDE 608

BLAST of MC05g0163 vs. ExPASy Swiss-Prot
Match: Q7UJ69 (Dihydroxy-acid dehydratase OS=Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1) OX=243090 GN=ilvD PE=3 SV=2)

HSP 1 Score: 787.3 bits (2032), Expect = 1.2e-226
Identity = 385/567 (67.90%), Postives = 463/567 (81.66%), Query Frame = 0

Query: 55  SPPPSSSAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCN 114
           S P S S A  LNKYSS++T+PKSQG SQA+L+  G+S +D+ KPQ+GI S+WYEGN+CN
Sbjct: 4   SQPESDSNA--LNKYSSKITQPKSQGASQAMLYATGMSSEDMNKPQVGIGSMWYEGNSCN 63

Query: 115 MHLLKLSEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSA 174
           MHLL L+  VK GV +AGMVG RFNTIGVSD ISMGT GM +SLQSRDLIADSIET+M A
Sbjct: 64  MHLLDLAADVKAGVTDAGMVGMRFNTIGVSDGISMGTDGMSFSLQSRDLIADSIETIMGA 123

Query: 175 QWYDGNISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEY 234
           QWYD  I++PGCDKNMPG +MAMGRLNRPSIMVYGGTIKPG ++    DIVSAFQ YG++
Sbjct: 124 QWYDALITLPGCDKNMPGCLMAMGRLNRPSIMVYGGTIKPGSYRDEKLDIVSAFQCYGQF 183

Query: 235 VSGSISDEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDE 294
           ++G IS+E+R  +VR SCPGAGACGGMYTANTMA+AIEA+GM+LPYS+S PAE P K +E
Sbjct: 184 IAGQISEEERSEIVRRSCPGAGACGGMYTANTMATAIEALGMALPYSASIPAEHPEKKEE 243

Query: 295 CRLAGKYLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTL 354
           C+ AG  +LELLK D+KPRDI+T  +  +AMV +MALGGSTNAVLHLIA+ARSV + LT+
Sbjct: 244 CKRAGLAILELLKKDIKPRDIMTRGAFEDAMVTLMALGGSTNAVLHLIAMARSVDVPLTI 303

Query: 355 DDFQKVSDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAE 414
           +DFQ VSD+ PFLADLKPSGK+V ED+H +GGTP V++YLLEKG++ G+ MTVTGKTLAE
Sbjct: 304 EDFQSVSDRTPFLADLKPSGKFVQEDLHSVGGTPAVMKYLLEKGMIKGEHMTVTGKTLAE 363

Query: 415 NAKIFLTLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFE 474
           N      L  GQ I+  +E PIKK+GHI+IL G+LA EG+VAKITGKEGL FSGPA V++
Sbjct: 364 NLADLPGLKTGQKIVSTVEEPIKKSGHIRILKGSLATEGAVAKITGKEGLQFSGPARVYD 423

Query: 475 GEESMIAAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGR 534
            EE M+AA+ E     KG VV+IR EGPKGGPGMPEMLTPTSAIMGAGLG +VA+LTDGR
Sbjct: 424 NEELMLAAL-EQKQIQKGDVVVIRYEGPKGGPGMPEMLTPTSAIMGAGLGSDVAMLTDGR 483

Query: 535 FSGGSHGFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPP 594
           FSGGSHGF+VGH+ PEAQ GGPI LI++GD IT+D +   +D+++   E+E RR+ W  P
Sbjct: 484 FSGGSHGFIVGHITPEAQVGGPIALIEDGDTITIDAETNSLDLEVDAAELEARRSKWTAP 543

Query: 595 AYKATRGILYKYIKNVQSASRGCVTDE 622
             KATRG LYKYIK V+SAS GCVTDE
Sbjct: 544 PLKATRGTLYKYIKCVKSASEGCVTDE 567

BLAST of MC05g0163 vs. ExPASy Swiss-Prot
Match: B0TZC0 (Dihydroxy-acid dehydratase OS=Francisella philomiragia subsp. philomiragia (strain ATCC 25017 / FSC 153 / O#319-036) OX=484022 GN=ilvD PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 3.2e-214
Identity = 362/555 (65.23%), Postives = 443/555 (79.82%), Query Frame = 0

Query: 66  LNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKLSEAVK 125
           LNKYS R+TE KSQG SQA+L+G G++D+D+ KPQIGI+SVWYEGNTCNMHL +L++ VK
Sbjct: 5   LNKYSRRLTEDKSQGASQAMLYGTGMNDNDMHKPQIGIASVWYEGNTCNMHLNQLAQFVK 64

Query: 126 EGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGNISIPG 185
             V++  + G RFNTIGVSD ISMGT GM YSLQSRDLIADSIETVMSA WYDG +SIPG
Sbjct: 65  NSVEKENLKGMRFNTIGVSDGISMGTDGMSYSLQSRDLIADSIETVMSAHWYDGLVSIPG 124

Query: 186 CDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSISDEQRR 245
           CDKNMPG +MA+GRLNRP  ++YGGTI+ G  +G   DIV+AFQ YG  +SG IS+++R+
Sbjct: 125 CDKNMPGCMMALGRLNRPGFVIYGGTIQAGVMRGKPIDIVTAFQSYGACLSGQISEQERQ 184

Query: 246 NVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGKYLLEL 305
             ++ +CPGAGACGGMYTANTMA AIEA+GMSLP+SSSTPA    K+ EC  AG+ +  L
Sbjct: 185 ETIKKACPGAGACGGMYTANTMACAIEALGMSLPFSSSTPATSVEKVQECDKAGETIKNL 244

Query: 306 LKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKVSDQVP 365
           L++D+KPRDI+T K+  NAMV++  +GGSTNAVLHL+A+A SV +DL +DDFQ+++++ P
Sbjct: 245 LELDIKPRDIMTRKAFENAMVLITVMGGSTNAVLHLLAMASSVDVDLNIDDFQEIANKTP 304

Query: 366 FLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFLTLSEG 425
            LAD KPSGKYVM D+H IGGTP V++ LL+ G+L GDC+TVTGKTLAEN +    L E 
Sbjct: 305 VLADFKPSGKYVMADLHAIGGTPAVMKMLLKAGMLHGDCLTVTGKTLAENLESVADLPED 364

Query: 426 QDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMIAAITE 485
             II  L+NPIKKTGH+QIL GN+APEGSVAKITGKEG  F G A VF+ EE M+AA+ E
Sbjct: 365 NTIIHKLDNPIKKTGHLQILKGNVAPEGSVAKITGKEGEVFEGVANVFDSEEEMVAAV-E 424

Query: 486 DPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSHGFVVG 545
           +    KG V++IR EGPKGGPGMPEML PTS IMGAGLG++VAL+TDGRFSGGSHGF+VG
Sbjct: 425 EGKVKKGDVIVIRYEGPKGGPGMPEMLKPTSLIMGAGLGQDVALITDGRFSGGSHGFIVG 484

Query: 546 HVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATRGILYK 605
           H+ PEA EGG + L++NGD IT+D  N  I+V LSDQE+ +R+  W  P  KATRG L K
Sbjct: 485 HIAPEAYEGGMLALLENGDKITIDAINNVINVDLSDQEIAQRKAKWQAPKQKATRGTLKK 544

Query: 606 YIKNVQSASRGCVTD 621
           YIK V SAS GCVTD
Sbjct: 545 YIKTVSSASTGCVTD 558

BLAST of MC05g0163 vs. ExPASy Swiss-Prot
Match: A0Q6R5 (Dihydroxy-acid dehydratase OS=Francisella tularensis subsp. novicida (strain U112) OX=401614 GN=ilvD PE=3 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 7.0e-214
Identity = 362/555 (65.23%), Postives = 444/555 (80.00%), Query Frame = 0

Query: 66  LNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKLSEAVK 125
           LNKYS R+TE KSQG SQA+L+G G++D D+ KPQIGI SVWYEGNTCNMHL +L++ VK
Sbjct: 5   LNKYSRRLTEDKSQGASQAMLYGTGMNDADMHKPQIGIGSVWYEGNTCNMHLNQLAQFVK 64

Query: 126 EGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGNISIPG 185
           + V++  + G RFNTIGVSD ISMGT GM YSLQSRDLIADSIETVMSA WYDG ISIPG
Sbjct: 65  DSVEKENLKGMRFNTIGVSDGISMGTDGMSYSLQSRDLIADSIETVMSAHWYDGLISIPG 124

Query: 186 CDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSISDEQRR 245
           CDKNMPG +MA+GRLNRP  ++YGGTI+ G  +G   DIV+AFQ YG  +SG I++++R+
Sbjct: 125 CDKNMPGCMMALGRLNRPGFVIYGGTIQAGVMRGKPIDIVTAFQSYGACLSGQITEQERQ 184

Query: 246 NVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGKYLLEL 305
             ++ +CPGAGACGGMYTANTMA AIEA+GMSLP+SSSTPA    K+ EC  AG+ +  L
Sbjct: 185 ETIKKACPGAGACGGMYTANTMACAIEALGMSLPFSSSTPATSVEKVQECDKAGETIKNL 244

Query: 306 LKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKVSDQVP 365
           L++D+KPRDI+T K+  NAMV++  +GGSTNAVLHL+A+A SV +DL++DDFQ+++++ P
Sbjct: 245 LELDIKPRDIMTRKAFENAMVLITVMGGSTNAVLHLLAMASSVDVDLSIDDFQEIANKTP 304

Query: 366 FLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFLTLSEG 425
            LAD KPSGKYVM D+H IGGTP V++ LL+ G+L GDC+TVTGKTLAEN +    L E 
Sbjct: 305 VLADFKPSGKYVMADLHAIGGTPAVMKMLLKAGMLHGDCLTVTGKTLAENLENVADLPED 364

Query: 426 QDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMIAAITE 485
             II  L+NPIKKTGH+QIL GN+APEGSVAKITGKEG  F G A VF+ EE M+AA+ E
Sbjct: 365 NTIIHKLDNPIKKTGHLQILKGNVAPEGSVAKITGKEGEVFEGVANVFDSEEEMVAAV-E 424

Query: 486 DPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSHGFVVG 545
           +    KG V++IR EGPKGGPGMPEML PTS IMGAGLG++VAL+TDGRFSGGSHGF+VG
Sbjct: 425 EGKVKKGDVIVIRYEGPKGGPGMPEMLKPTSLIMGAGLGQDVALITDGRFSGGSHGFIVG 484

Query: 546 HVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATRGILYK 605
           H+ PEA EGG I L++NGD IT+D  N  I+V LSDQE+ +R++ W  P  KA+RG L K
Sbjct: 485 HIAPEAYEGGMIALLENGDKITIDAINNVINVDLSDQEIAQRKSKWRAPKQKASRGTLKK 544

Query: 606 YIKNVQSASRGCVTD 621
           YIK V SAS GCVTD
Sbjct: 545 YIKTVSSASTGCVTD 558

BLAST of MC05g0163 vs. ExPASy Swiss-Prot
Match: B2SH83 (Dihydroxy-acid dehydratase OS=Francisella tularensis subsp. mediasiatica (strain FSC147) OX=441952 GN=ilvD PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 2.5e-211
Identity = 358/555 (64.50%), Postives = 442/555 (79.64%), Query Frame = 0

Query: 66  LNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKLSEAVK 125
           LNKYS R+TE KSQG SQA+L+G G++D D+ KPQIGI SVWYEGNTCNMHL +L++ VK
Sbjct: 5   LNKYSRRLTEDKSQGASQAMLYGTGMNDADMHKPQIGIGSVWYEGNTCNMHLNQLAQFVK 64

Query: 126 EGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGNISIPG 185
           + V++  + G RFNTIGVSD ISMGT  M YSLQSRDLIADSIETVMSA WYDG +SIPG
Sbjct: 65  DSVEKENLKGMRFNTIGVSDGISMGTDDMSYSLQSRDLIADSIETVMSAHWYDGLVSIPG 124

Query: 186 CDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSISDEQRR 245
           CDKNMPG +MA+GRLNRP  ++YGGTI+ G  +G   DIV+AFQ YG  +SG I++++R+
Sbjct: 125 CDKNMPGCMMALGRLNRPGFVIYGGTIQAGVMRGKPIDIVTAFQSYGACLSGQITEQERQ 184

Query: 246 NVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGKYLLEL 305
             ++ +CPGAGACGGMYTANTMA AIEA+GMSLP+SSSTPA    K+ EC  AG+ +  L
Sbjct: 185 ETIKKACPGAGACGGMYTANTMACAIEALGMSLPFSSSTPATSVEKVQECDKAGETIKNL 244

Query: 306 LKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKVSDQVP 365
           L++D+KPRDI+T K+  NAMV++  +GGSTNAVLHL+A+A SV +DL++DDFQ+++++ P
Sbjct: 245 LELDIKPRDIMTRKAFENAMVLITVMGGSTNAVLHLLAMASSVDVDLSIDDFQEIANKTP 304

Query: 366 FLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFLTLSEG 425
            LAD KPSGKYVM ++H IGGTP V++ LL+ G+L GDC+TVTGKTLAEN +    L E 
Sbjct: 305 VLADFKPSGKYVMANLHAIGGTPAVMKMLLKAGMLHGDCLTVTGKTLAENLENVADLPED 364

Query: 426 QDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMIAAITE 485
             II  L+NPIKKTGH+QIL GN+APEGSVAKITGKEG  F G A VF+ EE M+AA+ E
Sbjct: 365 NTIIHKLDNPIKKTGHLQILKGNVAPEGSVAKITGKEGEIFEGVANVFDSEEEMVAAV-E 424

Query: 486 DPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSHGFVVG 545
                KG V++IR EGPKGGPGMPEML PTS IMGAGLG++VAL+TDGRFSGGSHGF+VG
Sbjct: 425 TGKVKKGDVIVIRYEGPKGGPGMPEMLKPTSLIMGAGLGQDVALITDGRFSGGSHGFIVG 484

Query: 546 HVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATRGILYK 605
           H+ PEA EGG I L++NGD IT+D  N  I+V LSDQE+ +R++ W     KA+RG L K
Sbjct: 485 HITPEAYEGGMIALLENGDKITIDAINNVINVDLSDQEIAQRKSKWRASKQKASRGTLKK 544

Query: 606 YIKNVQSASRGCVTD 621
           YIK V SAS+GCVTD
Sbjct: 545 YIKTVSSASKGCVTD 558

BLAST of MC05g0163 vs. NCBI nr
Match: XP_022146997.1 (dihydroxy-acid dehydratase, chloroplastic [Momordica charantia])

HSP 1 Score: 1220 bits (3157), Expect = 0.0
Identity = 621/621 (100.00%), Postives = 621/621 (100.00%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS
Sbjct: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
           SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK
Sbjct: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL
Sbjct: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
           TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI
Sbjct: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH
Sbjct: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR
Sbjct: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 621
           GILYKYIKNVQSASRGCVTDE
Sbjct: 601 GILYKYIKNVQSASRGCVTDE 621

BLAST of MC05g0163 vs. NCBI nr
Match: XP_038904820.1 (dihydroxy-acid dehydratase, chloroplastic [Benincasa hispida])

HSP 1 Score: 1134 bits (2932), Expect = 0.0
Identity = 572/621 (92.11%), Postives = 597/621 (96.14%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQSAIIS +PRA  FI ISP VSTNV +RSRPL + ASISNVTTQ PPPSV+VDSP PSS
Sbjct: 1   MQSAIISSSPRAIPFITISPSVSTNV-QRSRPLVVSASISNVTTQSPPPSVSVDSPSPSS 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
           +  QKLNKYSSR+TEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  TTTQKLNKYSSRITEPKSQGGSQAILHGVGLSDDDLNKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVK GV+ AGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKYGVQAAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DE+RRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLK+DECRLAGK
Sbjct: 241 DEERRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKIDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SDQVP LADLKPSG+YVMED+HKIGGTP VIRYLLE GLLDGDC+TVTGKTLAENAK+FL
Sbjct: 361 SDQVPLLADLKPSGRYVMEDIHKIGGTPAVIRYLLEHGLLDGDCITVTGKTLAENAKLFL 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
            LSEGQDII+PLENPIKKTGH+QILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI
Sbjct: 421 PLSEGQDIIKPLENPIKKTGHLQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAIT+DPSSFKG+ VIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH
Sbjct: 481 AAITKDPSSFKGRTVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEA +GGPIGL+QNGD+I VDVQNRRIDVQLSDQE+EERR  WIPPAYKATR
Sbjct: 541 GFVVGHICPEALDGGPIGLVQNGDIINVDVQNRRIDVQLSDQEIEERRKKWIPPAYKATR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 621
           G+LYKYIKNVQ+AS+GCVTDE
Sbjct: 601 GVLYKYIKNVQAASKGCVTDE 620

BLAST of MC05g0163 vs. NCBI nr
Match: XP_004134011.1 (dihydroxy-acid dehydratase, chloroplastic [Cucumis sativus] >KGN56759.1 hypothetical protein Csa_009701 [Cucumis sativus])

HSP 1 Score: 1125 bits (2909), Expect = 0.0
Identity = 565/621 (90.98%), Postives = 594/621 (95.65%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQSAIISP PR+  F  I+P VSTNV +RSR L +RASISNVTTQ PPPSV VDSP PSS
Sbjct: 1   MQSAIISPLPRSIPFTTITPSVSTNV-QRSRSLVVRASISNVTTQSPPPSVVVDSPSPSS 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
           +AAQKLNKYSSR+TEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  TAAQKLNKYSSRITEPKSQGGSQAILHGVGLSDDDLNKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVK GV  AGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKYGVNAAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRPSIM+YGGTIKPGHFQGH YDIVSAFQVYGEYVSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMIYGGTIKPGHFQGHKYDIVSAFQVYGEYVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DE+R+NVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLK+DECRLAGK
Sbjct: 241 DEERKNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKIDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SDQVP L DLKPSGKYVMED+HKIGGTP VIRYLLE  LLDGDC+TVTGKTLAENAK+FL
Sbjct: 361 SDQVPLLGDLKPSGKYVMEDIHKIGGTPAVIRYLLENELLDGDCITVTGKTLAENAKLFL 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
            LSEGQDII+PLENPIKKTGH+QILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI
Sbjct: 421 PLSEGQDIIKPLENPIKKTGHLQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAIT+DPSSFKGK VIIRGEGPKGGPGMPEMLTPTSAIMGAGLGK+VALLTDGRFSGGSH
Sbjct: 481 AAITKDPSSFKGKTVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKDVALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEA +GGPIGL+QNGD+I VD++NRRIDVQLSDQEMEERR NW+PPAYKATR
Sbjct: 541 GFVVGHICPEALDGGPIGLVQNGDIINVDIENRRIDVQLSDQEMEERRKNWVPPAYKATR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 621
           G+LYKYIK+V++AS+GCVTDE
Sbjct: 601 GVLYKYIKSVKAASKGCVTDE 620

BLAST of MC05g0163 vs. NCBI nr
Match: XP_008438384.1 (PREDICTED: dihydroxy-acid dehydratase, chloroplastic [Cucumis melo] >KAA0049168.1 dihydroxy-acid dehydratase [Cucumis melo var. makuwa] >TYK17392.1 dihydroxy-acid dehydratase [Cucumis melo var. makuwa])

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 563/621 (90.66%), Postives = 595/621 (95.81%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQSAIISP+PR+  F  I+P VS+  ++RSR L +RASISNVTTQ PPPSV VDSP PSS
Sbjct: 1   MQSAIISPSPRSIPFTTITPSVSSTNVQRSRSLVVRASISNVTTQSPPPSVVVDSPSPSS 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
           +AAQKLNKYSSR+TEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  TAAQKLNKYSSRITEPKSQGGSQAILHGVGLSDDDLNKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVK GVK AGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKYGVKAAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DE+R+NVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLK+DECRLAGK
Sbjct: 241 DEERKNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKIDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGL+LTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLELTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SDQVP L DLKPSGKYVMED+HKIGGTP VIRYLLE  LLDGDC+TVTGKTLAENAK+FL
Sbjct: 361 SDQVPLLGDLKPSGKYVMEDIHKIGGTPAVIRYLLENELLDGDCITVTGKTLAENAKLFL 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
            LSEGQ+II+PLENPIK+TGH+QILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI
Sbjct: 421 PLSEGQEIIKPLENPIKETGHLQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAIT+DPSSFKGK VIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH
Sbjct: 481 AAITKDPSSFKGKTVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEA +GGPIGL+QNGD+I VD++NRRIDVQLSDQEMEERR NW+PPAYKATR
Sbjct: 541 GFVVGHICPEALDGGPIGLVQNGDIINVDIKNRRIDVQLSDQEMEERRKNWVPPAYKATR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 621
           G+LYKYIK+V+ AS+GCVTDE
Sbjct: 601 GVLYKYIKSVKPASKGCVTDE 621

BLAST of MC05g0163 vs. NCBI nr
Match: XP_022980256.1 (dihydroxy-acid dehydratase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1118 bits (2891), Expect = 0.0
Identity = 566/621 (91.14%), Postives = 590/621 (95.01%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQSAII+P+PRA  F  + PPV TNV RRSR LF+RAS SNVTT  PPP V+VDSP PSS
Sbjct: 1   MQSAIIAPSPRAIPFTTVRPPVHTNV-RRSRSLFVRASTSNVTTPSPPPPVSVDSPSPSS 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
             A KLNKYSSRVTEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  PGALKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLKKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVK GV  AGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKYGVNAAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQG+TYDIVSAFQVYGEYVSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGNTYDIVSAFQVYGEYVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DEQR++VVRNSCPGAGACGGMYTANTMASAIE MGMSLPYSSSTPAEDPLKLDECRLAGK
Sbjct: 241 DEQRKSVVRNSCPGAGACGGMYTANTMASAIETMGMSLPYSSSTPAEDPLKLDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLL+LLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV
Sbjct: 301 YLLDLLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SD+VPFLADLKPSGKYVMED+HKIGGTP VIRYLLE  LLDGDCMTVTGKTLAENAK+FL
Sbjct: 361 SDRVPFLADLKPSGKYVMEDIHKIGGTPAVIRYLLENELLDGDCMTVTGKTLAENAKLFL 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
            LSEGQD+IR L+NPIK+TGH+QILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEE+MI
Sbjct: 421 PLSEGQDVIRSLDNPIKETGHLQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEEAMI 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAITEDPSSFKG+ VIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH
Sbjct: 481 AAITEDPSSFKGRTVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEA EGGPIGL+QNGD+I VDVQNRRIDVQLSDQEMEERR NWI PAYKATR
Sbjct: 541 GFVVGHICPEAVEGGPIGLVQNGDIINVDVQNRRIDVQLSDQEMEERRKNWIRPAYKATR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 621
           G+L+KYIKNVQ+ASRGCVTDE
Sbjct: 601 GVLHKYIKNVQAASRGCVTDE 620

BLAST of MC05g0163 vs. ExPASy TrEMBL
Match: A0A6J1D152 (dihydroxy-acid dehydratase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016052 PE=3 SV=1)

HSP 1 Score: 1220 bits (3157), Expect = 0.0
Identity = 621/621 (100.00%), Postives = 621/621 (100.00%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS
Sbjct: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
           SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK
Sbjct: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL
Sbjct: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
           TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI
Sbjct: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH
Sbjct: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR
Sbjct: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 621
           GILYKYIKNVQSASRGCVTDE
Sbjct: 601 GILYKYIKNVQSASRGCVTDE 621

BLAST of MC05g0163 vs. ExPASy TrEMBL
Match: A0A0A0L6U0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G133100 PE=3 SV=1)

HSP 1 Score: 1125 bits (2909), Expect = 0.0
Identity = 565/621 (90.98%), Postives = 594/621 (95.65%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQSAIISP PR+  F  I+P VSTNV +RSR L +RASISNVTTQ PPPSV VDSP PSS
Sbjct: 1   MQSAIISPLPRSIPFTTITPSVSTNV-QRSRSLVVRASISNVTTQSPPPSVVVDSPSPSS 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
           +AAQKLNKYSSR+TEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  TAAQKLNKYSSRITEPKSQGGSQAILHGVGLSDDDLNKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVK GV  AGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKYGVNAAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRPSIM+YGGTIKPGHFQGH YDIVSAFQVYGEYVSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMIYGGTIKPGHFQGHKYDIVSAFQVYGEYVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DE+R+NVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLK+DECRLAGK
Sbjct: 241 DEERKNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKIDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SDQVP L DLKPSGKYVMED+HKIGGTP VIRYLLE  LLDGDC+TVTGKTLAENAK+FL
Sbjct: 361 SDQVPLLGDLKPSGKYVMEDIHKIGGTPAVIRYLLENELLDGDCITVTGKTLAENAKLFL 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
            LSEGQDII+PLENPIKKTGH+QILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI
Sbjct: 421 PLSEGQDIIKPLENPIKKTGHLQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAIT+DPSSFKGK VIIRGEGPKGGPGMPEMLTPTSAIMGAGLGK+VALLTDGRFSGGSH
Sbjct: 481 AAITKDPSSFKGKTVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKDVALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEA +GGPIGL+QNGD+I VD++NRRIDVQLSDQEMEERR NW+PPAYKATR
Sbjct: 541 GFVVGHICPEALDGGPIGLVQNGDIINVDIENRRIDVQLSDQEMEERRKNWVPPAYKATR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 621
           G+LYKYIK+V++AS+GCVTDE
Sbjct: 601 GVLYKYIKSVKAASKGCVTDE 620

BLAST of MC05g0163 vs. ExPASy TrEMBL
Match: A0A5A7U1Q9 (Dihydroxy-acid dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002520 PE=3 SV=1)

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 563/621 (90.66%), Postives = 595/621 (95.81%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQSAIISP+PR+  F  I+P VS+  ++RSR L +RASISNVTTQ PPPSV VDSP PSS
Sbjct: 1   MQSAIISPSPRSIPFTTITPSVSSTNVQRSRSLVVRASISNVTTQSPPPSVVVDSPSPSS 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
           +AAQKLNKYSSR+TEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  TAAQKLNKYSSRITEPKSQGGSQAILHGVGLSDDDLNKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVK GVK AGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKYGVKAAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DE+R+NVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLK+DECRLAGK
Sbjct: 241 DEERKNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKIDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGL+LTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLELTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SDQVP L DLKPSGKYVMED+HKIGGTP VIRYLLE  LLDGDC+TVTGKTLAENAK+FL
Sbjct: 361 SDQVPLLGDLKPSGKYVMEDIHKIGGTPAVIRYLLENELLDGDCITVTGKTLAENAKLFL 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
            LSEGQ+II+PLENPIK+TGH+QILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI
Sbjct: 421 PLSEGQEIIKPLENPIKETGHLQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAIT+DPSSFKGK VIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH
Sbjct: 481 AAITKDPSSFKGKTVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEA +GGPIGL+QNGD+I VD++NRRIDVQLSDQEMEERR NW+PPAYKATR
Sbjct: 541 GFVVGHICPEALDGGPIGLVQNGDIINVDIKNRRIDVQLSDQEMEERRKNWVPPAYKATR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 621
           G+LYKYIK+V+ AS+GCVTDE
Sbjct: 601 GVLYKYIKSVKPASKGCVTDE 621

BLAST of MC05g0163 vs. ExPASy TrEMBL
Match: A0A1S3AVX2 (dihydroxy-acid dehydratase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483500 PE=3 SV=1)

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 563/621 (90.66%), Postives = 595/621 (95.81%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQSAIISP+PR+  F  I+P VS+  ++RSR L +RASISNVTTQ PPPSV VDSP PSS
Sbjct: 1   MQSAIISPSPRSIPFTTITPSVSSTNVQRSRSLVVRASISNVTTQSPPPSVVVDSPSPSS 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
           +AAQKLNKYSSR+TEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  TAAQKLNKYSSRITEPKSQGGSQAILHGVGLSDDDLNKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVK GVK AGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKYGVKAAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DE+R+NVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLK+DECRLAGK
Sbjct: 241 DEERKNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKIDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGL+LTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLELTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SDQVP L DLKPSGKYVMED+HKIGGTP VIRYLLE  LLDGDC+TVTGKTLAENAK+FL
Sbjct: 361 SDQVPLLGDLKPSGKYVMEDIHKIGGTPAVIRYLLENELLDGDCITVTGKTLAENAKLFL 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
            LSEGQ+II+PLENPIK+TGH+QILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI
Sbjct: 421 PLSEGQEIIKPLENPIKETGHLQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAIT+DPSSFKGK VIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH
Sbjct: 481 AAITKDPSSFKGKTVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEA +GGPIGL+QNGD+I VD++NRRIDVQLSDQEMEERR NW+PPAYKATR
Sbjct: 541 GFVVGHICPEALDGGPIGLVQNGDIINVDIKNRRIDVQLSDQEMEERRKNWVPPAYKATR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 621
           G+LYKYIK+V+ AS+GCVTDE
Sbjct: 601 GVLYKYIKSVKPASKGCVTDE 621

BLAST of MC05g0163 vs. ExPASy TrEMBL
Match: A0A6J1IT44 (dihydroxy-acid dehydratase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479678 PE=3 SV=1)

HSP 1 Score: 1118 bits (2891), Expect = 0.0
Identity = 566/621 (91.14%), Postives = 590/621 (95.01%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQSAII+P+PRA  F  + PPV TNV RRSR LF+RAS SNVTT  PPP V+VDSP PSS
Sbjct: 1   MQSAIIAPSPRAIPFTTVRPPVHTNV-RRSRSLFVRASTSNVTTPSPPPPVSVDSPSPSS 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
             A KLNKYSSRVTEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  PGALKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLKKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVK GV  AGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKYGVNAAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQG+TYDIVSAFQVYGEYVSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGNTYDIVSAFQVYGEYVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DEQR++VVRNSCPGAGACGGMYTANTMASAIE MGMSLPYSSSTPAEDPLKLDECRLAGK
Sbjct: 241 DEQRKSVVRNSCPGAGACGGMYTANTMASAIETMGMSLPYSSSTPAEDPLKLDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLL+LLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV
Sbjct: 301 YLLDLLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SD+VPFLADLKPSGKYVMED+HKIGGTP VIRYLLE  LLDGDCMTVTGKTLAENAK+FL
Sbjct: 361 SDRVPFLADLKPSGKYVMEDIHKIGGTPAVIRYLLENELLDGDCMTVTGKTLAENAKLFL 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
            LSEGQD+IR L+NPIK+TGH+QILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEE+MI
Sbjct: 421 PLSEGQDVIRSLDNPIKETGHLQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEEAMI 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAITEDPSSFKG+ VIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH
Sbjct: 481 AAITEDPSSFKGRTVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEA EGGPIGL+QNGD+I VDVQNRRIDVQLSDQEMEERR NWI PAYKATR
Sbjct: 541 GFVVGHICPEAVEGGPIGLVQNGDIINVDVQNRRIDVQLSDQEMEERRKNWIRPAYKATR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 621
           G+L+KYIKNVQ+ASRGCVTDE
Sbjct: 601 GVLHKYIKNVQAASRGCVTDE 620

BLAST of MC05g0163 vs. TAIR 10
Match: AT3G23940.1 (dehydratase family )

HSP 1 Score: 1011.5 bits (2614), Expect = 2.9e-295
Identity = 507/621 (81.64%), Postives = 548/621 (88.24%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQ+ I S  PRAT F P  P + ++ +   RP  +  S  +VT  P PP    +      
Sbjct: 1   MQATIFS--PRATLF-PCKPLLPSHNVNSRRPSIISCSAQSVTADPSPPITDTN------ 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
               KLNKYSSR+TEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  ----KLNKYSSRITEPKSQGGSQAILHGVGLSDDDLLKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVKEGV+ AGMVGFRFNTIGVSDAISMGTRGMC+SLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKEGVENAGMVGFRFNTIGVSDAISMGTRGMCFSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRP IMVYGGTIKPGHFQ  TYDIVSAFQ YGE+VSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPGIMVYGGTIKPGHFQDKTYDIVSAFQSYGEFVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DEQR+ V+ +SCPGAGACGGMYTANTMASAIEAMGMSLPYSSS PAEDPLKLDECRLAGK
Sbjct: 241 DEQRKTVLHHSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSIPAEDPLKLDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIIT KSLRNAMV VMALGGSTNAVLHLIAIARSVGL+LTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITPKSLRNAMVSVMALGGSTNAVLHLIAIARSVGLELTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SD VPFLADLKPSGKYVMED+HKIGGTP V+RYLLE GL+DGDCMTVTG+TLA+N +   
Sbjct: 361 SDAVPFLADLKPSGKYVMEDIHKIGGTPAVLRYLLELGLMDGDCMTVTGQTLAQNLENVP 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
           +L+EGQ+IIRPL NPIK+TGHIQIL G+LAP+GSVAKITGKEGLYFSGPALVFEGEESM+
Sbjct: 421 SLTEGQEIIRPLSNPIKETGHIQILRGDLAPDGSVAKITGKEGLYFSGPALVFEGEESML 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAI+ DP SFKG VV+IRGEGPKGGPGMPEMLTPTSAIMGAGLGKE ALLTDGRFSGGSH
Sbjct: 481 AAISADPMSFKGTVVVIRGEGPKGGPGMPEMLTPTSAIMGAGLGKECALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEAQEGGPIGLI+NGD+IT+D+  +RID Q+S +EM +RR  W  PAYK  R
Sbjct: 541 GFVVGHICPEAQEGGPIGLIKNGDIITIDIGKKRIDTQVSPEEMNDRRKKWTAPAYKVNR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 622
           G+LYKYIKNVQSAS GCVTDE
Sbjct: 601 GVLYKYIKNVQSASDGCVTDE 608

BLAST of MC05g0163 vs. TAIR 10
Match: AT3G23940.2 (dehydratase family )

HSP 1 Score: 1003.0 bits (2592), Expect = 1.0e-292
Identity = 505/621 (81.32%), Postives = 546/621 (87.92%), Query Frame = 0

Query: 1   MQSAIISPAPRATTFIPISPPVSTNVLRRSRPLFLRASISNVTTQPPPPSVTVDSPPPSS 60
           MQ+ I S  PRAT F P  P + ++ +   RP  +  S  +VT  P PP    +      
Sbjct: 1   MQATIFS--PRATLF-PCKPLLPSHNVNSRRPSIISCSAQSVTADPSPPITDTN------ 60

Query: 61  SAAQKLNKYSSRVTEPKSQGGSQAILHGVGLSDDDLTKPQIGISSVWYEGNTCNMHLLKL 120
               KLNKYSSR+TEPKSQGGSQAILHGVGLSDDDL KPQIGISSVWYEGNTCNMHLLKL
Sbjct: 61  ----KLNKYSSRITEPKSQGGSQAILHGVGLSDDDLLKPQIGISSVWYEGNTCNMHLLKL 120

Query: 121 SEAVKEGVKEAGMVGFRFNTIGVSDAISMGTRGMCYSLQSRDLIADSIETVMSAQWYDGN 180
           SEAVKEGV+ AGMVGFRFNTIGVSDAISMGTRGMC+SLQSRDLIADSIETVMSAQWYDGN
Sbjct: 121 SEAVKEGVENAGMVGFRFNTIGVSDAISMGTRGMCFSLQSRDLIADSIETVMSAQWYDGN 180

Query: 181 ISIPGCDKNMPGTIMAMGRLNRPSIMVYGGTIKPGHFQGHTYDIVSAFQVYGEYVSGSIS 240
           ISIPGCDKNMPGTIMAMGRLNRP IMVYGGTIKPGHFQ  TYDIVSAFQ YGE+VSGSIS
Sbjct: 181 ISIPGCDKNMPGTIMAMGRLNRPGIMVYGGTIKPGHFQDKTYDIVSAFQSYGEFVSGSIS 240

Query: 241 DEQRRNVVRNSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSTPAEDPLKLDECRLAGK 300
           DEQR+ V+ +SCPGAGACGGMYTANTMASAIEAMGMSLPYSSS PAEDPLKLDECRLAGK
Sbjct: 241 DEQRKTVLHHSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSIPAEDPLKLDECRLAGK 300

Query: 301 YLLELLKMDLKPRDIITEKSLRNAMVVVMALGGSTNAVLHLIAIARSVGLDLTLDDFQKV 360
           YLLELLKMDLKPRDIIT KSLRNAMV VMALGGSTNAVLHLIAIARSVGL+LTLDDFQKV
Sbjct: 301 YLLELLKMDLKPRDIITPKSLRNAMVSVMALGGSTNAVLHLIAIARSVGLELTLDDFQKV 360

Query: 361 SDQVPFLADLKPSGKYVMEDVHKIGGTPGVIRYLLEKGLLDGDCMTVTGKTLAENAKIFL 420
           SD VPFLADLKPSGKYVMED+HKIGGTP V+RYLLE GL+DGDCM  TG+TLA+N +   
Sbjct: 361 SDAVPFLADLKPSGKYVMEDIHKIGGTPAVLRYLLELGLMDGDCM--TGQTLAQNLENVP 420

Query: 421 TLSEGQDIIRPLENPIKKTGHIQILYGNLAPEGSVAKITGKEGLYFSGPALVFEGEESMI 480
           +L+EGQ+IIRPL NPIK+TGHIQIL G+LAP+GSVAKITGKEGLYFSGPALVFEGEESM+
Sbjct: 421 SLTEGQEIIRPLSNPIKETGHIQILRGDLAPDGSVAKITGKEGLYFSGPALVFEGEESML 480

Query: 481 AAITEDPSSFKGKVVIIRGEGPKGGPGMPEMLTPTSAIMGAGLGKEVALLTDGRFSGGSH 540
           AAI+ DP SFKG VV+IRGEGPKGGPGMPEMLTPTSAIMGAGLGKE ALLTDGRFSGGSH
Sbjct: 481 AAISADPMSFKGTVVVIRGEGPKGGPGMPEMLTPTSAIMGAGLGKECALLTDGRFSGGSH 540

Query: 541 GFVVGHVCPEAQEGGPIGLIQNGDVITVDVQNRRIDVQLSDQEMEERRNNWIPPAYKATR 600
           GFVVGH+CPEAQEGGPIGLI+NGD+IT+D+  +RID Q+S +EM +RR  W  PAYK  R
Sbjct: 541 GFVVGHICPEAQEGGPIGLIKNGDIITIDIGKKRIDTQVSPEEMNDRRKKWTAPAYKVNR 600

Query: 601 GILYKYIKNVQSASRGCVTDE 622
           G+LYKYIKNVQSAS GCVTDE
Sbjct: 601 GVLYKYIKNVQSASDGCVTDE 606

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIR44.0e-29481.64Dihydroxy-acid dehydratase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DHA... [more]
Q7UJ691.2e-22667.90Dihydroxy-acid dehydratase OS=Rhodopirellula baltica (strain DSM 10527 / NCIMB 1... [more]
B0TZC03.2e-21465.23Dihydroxy-acid dehydratase OS=Francisella philomiragia subsp. philomiragia (stra... [more]
A0Q6R57.0e-21465.23Dihydroxy-acid dehydratase OS=Francisella tularensis subsp. novicida (strain U11... [more]
B2SH832.5e-21164.50Dihydroxy-acid dehydratase OS=Francisella tularensis subsp. mediasiatica (strain... [more]
Match NameE-valueIdentityDescription
XP_022146997.10.0100.00dihydroxy-acid dehydratase, chloroplastic [Momordica charantia][more]
XP_038904820.10.092.11dihydroxy-acid dehydratase, chloroplastic [Benincasa hispida][more]
XP_004134011.10.090.98dihydroxy-acid dehydratase, chloroplastic [Cucumis sativus] >KGN56759.1 hypothet... [more]
XP_008438384.10.090.66PREDICTED: dihydroxy-acid dehydratase, chloroplastic [Cucumis melo] >KAA0049168.... [more]
XP_022980256.10.091.14dihydroxy-acid dehydratase, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1D1520.0100.00dihydroxy-acid dehydratase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A0A0L6U00.090.98Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G133100 PE=3 SV=1[more]
A0A5A7U1Q90.090.66Dihydroxy-acid dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3AVX20.090.66dihydroxy-acid dehydratase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10348350... [more]
A0A6J1IT440.091.14dihydroxy-acid dehydratase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT3G23940.12.9e-29581.64dehydratase family [more]
AT3G23940.21.0e-29281.32dehydratase family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042096Dihydroxy-acid dehydratase, C-terminalGENE3D3.50.30.80coord: 441..591
e-value: 1.3E-56
score: 192.4
IPR000581Dihydroxy-acid/6-phosphogluconate dehydratasePFAMPF00920ILVD_EDDcoord: 98..617
e-value: 7.0E-217
score: 721.1
IPR004404Dihydroxy-acid dehydrataseTIGRFAMTIGR00110TIGR00110coord: 82..620
e-value: 1.6E-226
score: 751.0
IPR004404Dihydroxy-acid dehydrataseHAMAPMF_00012IlvDcoord: 68..620
score: 42.290825
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..81
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..82
NoneNo IPR availablePANTHERPTHR21000DIHYDROXY-ACID DEHYDRATASE DADcoord: 10..621
NoneNo IPR availablePANTHERPTHR21000:SF14BNAA01G23200D PROTEINcoord: 10..621
NoneNo IPR availableSUPERFAMILY52016LeuD/IlvD-likecoord: 442..620
IPR020558Dihydroxy-acid/6-phosphogluconate dehydratase, conserved sitePROSITEPS00887ILVD_EDD_2coord: 528..539
IPR020558Dihydroxy-acid/6-phosphogluconate dehydratase, conserved sitePROSITEPS00886ILVD_EDD_1coord: 186..196
IPR037237IlvD/EDD, N-terminal domainSUPERFAMILY143975IlvD/EDD N-terminal domain-likecoord: 61..440

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0163.1MC05g0163.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009082 branched-chain amino acid biosynthetic process
biological_process GO:0008652 cellular amino acid biosynthetic process
biological_process GO:0009553 embryo sac development
biological_process GO:0009555 pollen development
biological_process GO:0009651 response to salt stress
biological_process GO:0048364 root development
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0004160 dihydroxy-acid dehydratase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity