MC05g0155 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0155 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 4.6e-199
Identity = 345/468 (73.72%), Postives = 398/468 (85.04%), Query Frame = 0

Query: 4   AASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMA----VRLDEVE 63
           ++S K I + + V+ +L +  +     EIS ++   TE++QSS++S+MA    ++ +E +
Sbjct: 3   SSSQKLISVCVAVLVVLALTAMIFRNSEISLSRKLKTEVIQSSNSSTMAAIRKLKTEEFQ 62

Query: 64  ALDN---------------KHAV-DNP----EEIAAMVDMSIRNATARRELGFFSCGTGN 123
           +L++               +HAV D+P    +E+A +V MS +N TARR+LGFFSCGTGN
Sbjct: 63  SLNSSTMAATRLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFSCGTGN 122

Query: 124 PIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHA 183
           PIDDCWRCD NW +NRKRLADCGIGFGRNAIGGRDGRFY+VTD  D++ VNP PGTLRHA
Sbjct: 123 PIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHA 182

Query: 184 VIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLN 243
           VIQ+EPLWIVFKRDMVI+LKQELIMNSFKTIDARG+NVHIANGACITIQF+TNVI+HGL+
Sbjct: 183 VIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLH 242

Query: 244 IHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGST 303
           IHDCKPTGNAMVRSSP+H GWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMGST
Sbjct: 243 IHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGST 302

Query: 304 AITITSNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNN 363
           AIT+++N+FTHHNEVMLLGHSDSY +DK MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNN
Sbjct: 303 AITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNN 362

Query: 364 DYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLL 423
           DYTHWEMYAIGGSA PTINSQGNRYAAP + FAKEVTKRVET  +EWK WNWRSEGDLLL
Sbjct: 363 DYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLL 422

Query: 424 NGAFFTPSGAGASASYARASSLGAKSSSMVASITSSAGALSCRRGHPC 448
           NGAFF PSGAGASASY RASSL AK SSMV +ITS+AGAL CR+G PC
Sbjct: 423 NGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

BLAST of MC05g0155 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 694.1 bits (1790), Expect = 1.0e-198
Identity = 336/404 (83.17%), Postives = 358/404 (88.61%), Query Frame = 0

Query: 48  NSSMAVRLDEVEALDNKHAVDNPEEIA----AMVDMSIRNATARRELGFFSCGTGNPIDD 107
           N S   R DE E   N+HAV NP+E+A    A+ +MS+RN T RR+LG+F+CGTGNPIDD
Sbjct: 28  NISSLPRSDETEW--NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDD 87

Query: 108 CWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQD 167
           CWRCDPNW +NRKRLADCGIGFGRNAIGGRDGRFYVVTD  DDNPVNP PGTLRHAVIQD
Sbjct: 88  CWRCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQD 147

Query: 168 EPLWIVFKRDMVIQLKQELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDC 227
            PLWIVFKRDMVIQLKQELI+NSFKTID RGANVHIANG CITIQFVTNVIVHGL+IHDC
Sbjct: 148 RPLWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDC 207

Query: 228 KPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI 287
           KPTGNAMVRSS  H GWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITI
Sbjct: 208 KPTGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITI 267

Query: 288 TSNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTH 347
           ++N+ THHNEVMLLGHSDSY+RDK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTH
Sbjct: 268 SNNHLTHHNEVMLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTH 327

Query: 348 WEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAF 407
           WEMYAIGGSANPTINSQGNRYAAP NPFAKEVTKRV+T  + WK WNWRSEGDLL NGA+
Sbjct: 328 WEMYAIGGSANPTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAY 387

Query: 408 FTPSGAGASASYARASSLGAKSSSMVASITSSAGALSCRRGHPC 448
           FT SGA AS SYARASSL AKSSS+V  ITS AGAL CRRG  C
Sbjct: 388 FTSSGAAASGSYARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of MC05g0155 vs. ExPASy Swiss-Prot
Match: Q9M8Z8 (Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 3.6e-196
Identity = 321/385 (83.38%), Postives = 353/385 (91.69%), Query Frame = 0

Query: 63  NKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122
           ++HAV+NP+E+AAMVDMSIRN+T RR LG+FSC TGNPIDDCWRCD  WQ  RKRLADC 
Sbjct: 32  HEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCS 91

Query: 123 IGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 182
           IGFGRNAIGGRDGRFYVVTD GDD+PVNP+PGTLRHAVIQDEPLWI+FKRDMVI LKQEL
Sbjct: 92  IGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQEL 151

Query: 183 IMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242
           IMNSFKTID RG NVHIANGAC+TIQ+VTN+IVHG+++HDCKPTGNAMVRSSP+H G+R+
Sbjct: 152 IMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRS 211

Query: 243 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSDS 302
           MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT+++N+FTHHNEVMLLGHSDS
Sbjct: 212 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLLGHSDS 271

Query: 303 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGN
Sbjct: 272 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTINSQGN 331

Query: 363 RYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSLG 422
           R+ AP NPFAKEVTKR  T E++WK WNWRSEGDL LNGAFFT SGAGA A+YARASSL 
Sbjct: 332 RFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYARASSLS 391

Query: 423 AKSSSMVASITSSAGALSCRRGHPC 448
           AKSSS+V ++TS +GAL+CR G  C
Sbjct: 392 AKSSSLVGTMTSYSGALNCRAGRRC 416

BLAST of MC05g0155 vs. ExPASy Swiss-Prot
Match: Q93WF1 (Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 6.4e-193
Identity = 317/385 (82.34%), Postives = 346/385 (89.87%), Query Frame = 0

Query: 63  NKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122
           ++HAV+NPEE+AAMVDMSIRN+TARR LG+FSC TGNPIDDCWRCD  WQ  RK LA+C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 123 IGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 182
           IGFGRNAIGGRDGR+YVV+D  DDNPVNP PGTLRHAVIQ+EPLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 183 IMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242
           IMNSFKTID RG NVHIANGACITIQFVTN+I+HG++IHDC+PTGNAMVRSSP+H GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 243 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSDS 302
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITI++NYFTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 303 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 363 RYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSLG 422
           RY AP N FAKEVTKR    + +W+ WNWRSEGDL LNGAFFT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 423 AKSSSMVASITSSAGALSCRRGHPC 448
           AKSSS+V  IT +AGAL+CR G  C
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

BLAST of MC05g0155 vs. ExPASy Swiss-Prot
Match: Q9LJ42 (Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2)

HSP 1 Score: 668.3 bits (1723), Expect = 6.0e-191
Identity = 324/417 (77.70%), Postives = 356/417 (85.37%), Query Frame = 0

Query: 33  SQTQNGATEML--QSSSNSSMAVRLDEVEALDNKHAVDNPEEIAAMVDMSIRNATARREL 92
           S T    TE L   SSSNSS A +L   +   N+HAV NPEE+AAMVDM I+N+T RR L
Sbjct: 24  SSTMAQETEDLNSHSSSNSSTANKLPNDDGAWNEHAVKNPEEVAAMVDMKIKNSTERRRL 83

Query: 93  GFFSCGTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVVTDSGDDNPVN 152
           GFFSC TGNPIDDCWRCD NW   RKRLA+C IGFGRNAIGGRDGR+YVVTD  D + VN
Sbjct: 84  GFFSCATGNPIDDCWRCDRNWHLRRKRLANCAIGFGRNAIGGRDGRYYVVTDPSDHDAVN 143

Query: 153 PMPGTLRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGANVHIANGACITIQFV 212
           P PGTLRHAVIQD PLWIVFKRDMVI L QELIMNSFKTID RG NV IA GACITIQ+V
Sbjct: 144 PRPGTLRHAVIQDRPLWIVFKRDMVITLTQELIMNSFKTIDGRGVNVAIAGGACITIQYV 203

Query: 213 TNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADG 272
           TN+I+HG+N+HDC+ TGNAMVRSSP+H GWRTMADGDAISIFGSSHIWIDHNSLSNCADG
Sbjct: 204 TNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGDAISIFGSSHIWIDHNSLSNCADG 263

Query: 273 LVDAVMGSTAITITSNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCR 332
           L+DA+MGSTAITI++NY THHNEVML+GHSDSY RDK MQVTIAYNHFGEGLIQRMPRCR
Sbjct: 264 LIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDKLMQVTIAYNHFGEGLIQRMPRCR 323

Query: 333 HGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAETEWKSWN 392
           HGYFHVVNNDYTHW MYAIGGSANPTINSQGNR+ AP NPFAKEVTKRV + + EWK WN
Sbjct: 324 HGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAPGNPFAKEVTKRVGSWQGEWKQWN 383

Query: 393 WRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMVASITSSAGALSCRRGHPC 448
           WRS+GDL+LNGA+FT SGA A ASYARASSLGAK +S+V+ +T S+GAL CR G  C
Sbjct: 384 WRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPASVVSMLTYSSGALKCRIGMRC 440

BLAST of MC05g0155 vs. NCBI nr
Match: XP_022147150.1 (probable pectate lyase 8 [Momordica charantia])

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   VAMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEA 60
           VAMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEA
Sbjct: 66  VAMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEA 125

Query: 61  LDNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120
           LDNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD
Sbjct: 126 LDNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 185

Query: 121 CGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180
           CGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQ
Sbjct: 186 CGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 245

Query: 181 ELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240
           ELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW
Sbjct: 246 ELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 305

Query: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHS 300
           RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHS
Sbjct: 306 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHS 365

Query: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360
           DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ
Sbjct: 366 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 425

Query: 361 GNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASS 420
           GNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASS
Sbjct: 426 GNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASS 485

Query: 421 LGAKSSSMVASITSSAGALSCRRGHPC 447
           LGAKSSSMVASITSSAGALSCRRGHPC
Sbjct: 486 LGAKSSSMVASITSSAGALSCRRGHPC 512

BLAST of MC05g0155 vs. NCBI nr
Match: XP_008438392.1 (PREDICTED: probable pectate lyase 8 [Cucumis melo] >KAA0049176.1 putative pectate lyase 8 [Cucumis melo var. makuwa] >TYK17384.1 putative pectate lyase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 862 bits (2228), Expect = 3.44e-315
Identity = 418/446 (93.72%), Postives = 433/446 (97.09%), Query Frame = 0

Query: 2   AMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEAL 61
           AMA SGKWIRL+LLV+FMLFVG IARVPKEI +T+NG +E LQSSSNS+MAVRLDE + L
Sbjct: 2   AMAESGKWIRLILLVMFMLFVGAIARVPKEIPETENGGSEKLQSSSNSTMAVRLDETKDL 61

Query: 62  DNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121
            N+HAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC
Sbjct: 62  GNEHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121

Query: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181
           GIGFGRNAIGGRDGRFYVVTDSGDDNPVNP PGTLRHAVIQDEPLWIVFKRDMVIQLKQE
Sbjct: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPKPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181

Query: 182 LIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241
           LIMNSFKTIDARG NVHIANGAC+TIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR
Sbjct: 182 LIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241

Query: 242 TMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSD 301
           T+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI++NYFTHHNEVMLLGHSD
Sbjct: 242 TIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNYFTHHNEVMLLGHSD 301

Query: 302 SYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361
           SYVRDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG
Sbjct: 302 SYVRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361

Query: 362 NRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSL 421
           NRYAAPTNPFAKEVTKRVETAE+EWK WNWRSEGDLLLNGAFFTPSGAGASASYARASSL
Sbjct: 362 NRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDLLLNGAFFTPSGAGASASYARASSL 421

Query: 422 GAKSSSMVASITSSAGALSCRRGHPC 447
           GAKSSSMV +ITS+AGALSCRRGHPC
Sbjct: 422 GAKSSSMVGTITSNAGALSCRRGHPC 447

BLAST of MC05g0155 vs. NCBI nr
Match: XP_038895013.1 (probable pectate lyase 8 [Benincasa hispida])

HSP 1 Score: 860 bits (2221), Expect = 3.78e-314
Identity = 420/445 (94.38%), Postives = 429/445 (96.40%), Query Frame = 0

Query: 3   MAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEALD 62
           MA SG W RL LLVVFMLFVGTIARVPKEI + QNGATE ++SSSNSSMAVRLDE EAL 
Sbjct: 3   MAESGGWTRLFLLVVFMLFVGTIARVPKEIPEIQNGATEKMRSSSNSSMAVRLDEAEALG 62

Query: 63  NKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122
           NKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG
Sbjct: 63  NKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122

Query: 123 IGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 182
           IGFGRNAIGGRDGRFY+VTDSGDDNPVNP PGTLRHAVIQDEPLWIVFKRDMVI LKQEL
Sbjct: 123 IGFGRNAIGGRDGRFYMVTDSGDDNPVNPKPGTLRHAVIQDEPLWIVFKRDMVIHLKQEL 182

Query: 183 IMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242
           IMNSFKTIDARG NVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT
Sbjct: 183 IMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242

Query: 243 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSDS 302
           +ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI++NYFTHHNEVMLLGHSDS
Sbjct: 243 IADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNYFTHHNEVMLLGHSDS 302

Query: 303 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362
           YVRDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN
Sbjct: 303 YVRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362

Query: 363 RYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSLG 422
           RYAAPTNPFAKEVTKRVETAETEWK+WNWRSEGDLLLNGAFFTPSGAGASASYARASSLG
Sbjct: 363 RYAAPTNPFAKEVTKRVETAETEWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLG 422

Query: 423 AKSSSMVASITSSAGALSCRRGHPC 447
           AKSSSMV +ITS AGALSCRRGH C
Sbjct: 423 AKSSSMVGTITSDAGALSCRRGHRC 447

BLAST of MC05g0155 vs. NCBI nr
Match: XP_004134015.1 (probable pectate lyase 8 [Cucumis sativus] >KGN56767.1 hypothetical protein Csa_010551 [Cucumis sativus])

HSP 1 Score: 853 bits (2205), Expect = 1.05e-311
Identity = 414/446 (92.83%), Postives = 429/446 (96.19%), Query Frame = 0

Query: 2   AMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEAL 61
           AMA SGKWIRL+LLVVFMLFVG IAR PKEI + +NG TE LQSSSNS+MAVRLDE + L
Sbjct: 2   AMAESGKWIRLILLVVFMLFVGAIARAPKEIPEIENGGTEKLQSSSNSTMAVRLDEAKDL 61

Query: 62  DNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121
            N+HAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC
Sbjct: 62  GNEHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121

Query: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181
           GIGFGRNAIGGRDGRFYVVTDSGDDNPVNP PGTLRHAVIQDEPLWIVFKRDMVIQLKQE
Sbjct: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPKPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181

Query: 182 LIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241
           LIMNSFKTIDARG NVHIANGAC+TIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR
Sbjct: 182 LIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241

Query: 242 TMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSD 301
           T+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI++NYFTHHNEVMLLGHSD
Sbjct: 242 TIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNYFTHHNEVMLLGHSD 301

Query: 302 SYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361
           SYVRDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG
Sbjct: 302 SYVRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361

Query: 362 NRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSL 421
           NRYAAPTNPFAKEVTKRVET+E+EWK WNWRSEGDLLLNGAFF PSGAGAS+SYARASSL
Sbjct: 362 NRYAAPTNPFAKEVTKRVETSESEWKGWNWRSEGDLLLNGAFFIPSGAGASSSYARASSL 421

Query: 422 GAKSSSMVASITSSAGALSCRRGHPC 447
           GAKSSSMV +ITS+AGALSCRRGH C
Sbjct: 422 GAKSSSMVGTITSNAGALSCRRGHAC 447

BLAST of MC05g0155 vs. NCBI nr
Match: KAG6597311.1 (putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 853 bits (2203), Expect = 2.11e-311
Identity = 415/446 (93.05%), Postives = 433/446 (97.09%), Query Frame = 0

Query: 2   AMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEAL 61
           AMA SG WIRL LLVVFMLFV TIARVPKEI + +N ATE+L+SSSN+SMA RL++VEAL
Sbjct: 2   AMAESGGWIRLFLLVVFMLFVETIARVPKEIPEIENRATEILRSSSNASMAERLEDVEAL 61

Query: 62  DNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121
            NKHAVDNPEE+AA VDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC
Sbjct: 62  GNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121

Query: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181
           GIGFGRNAIGGRDGR+YVVTDSG+DNPV+P PGTLRHAVIQDEPLWIVFKRDMVIQLKQE
Sbjct: 122 GIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181

Query: 182 LIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241
           LIMNSFKTIDARG NVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR
Sbjct: 182 LIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241

Query: 242 TMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSD 301
           TMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITIT+NYFTHHNEVMLLGHSD
Sbjct: 242 TMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGHSD 301

Query: 302 SYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361
           SYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG
Sbjct: 302 SYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361

Query: 362 NRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSL 421
           NRYAAPTNPFAKEVTKRVETAE+EWK+WNWRSEGDLL+NGAFFTPSGAGASASYARASSL
Sbjct: 362 NRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSL 421

Query: 422 GAKSSSMVASITSSAGALSCRRGHPC 447
           GAKSSSMVASIT++AGAL+CRRGH C
Sbjct: 422 GAKSSSMVASITANAGALNCRRGHAC 447

BLAST of MC05g0155 vs. ExPASy TrEMBL
Match: A0A6J1CZC3 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111016150 PE=3 SV=1)

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   VAMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEA 60
           VAMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEA
Sbjct: 66  VAMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEA 125

Query: 61  LDNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 120
           LDNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD
Sbjct: 126 LDNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLAD 185

Query: 121 CGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 180
           CGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQ
Sbjct: 186 CGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQ 245

Query: 181 ELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 240
           ELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW
Sbjct: 246 ELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGW 305

Query: 241 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHS 300
           RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHS
Sbjct: 306 RTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHS 365

Query: 301 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 360
           DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ
Sbjct: 366 DSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 425

Query: 361 GNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASS 420
           GNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASS
Sbjct: 426 GNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASS 485

Query: 421 LGAKSSSMVASITSSAGALSCRRGHPC 447
           LGAKSSSMVASITSSAGALSCRRGHPC
Sbjct: 486 LGAKSSSMVASITSSAGALSCRRGHPC 512

BLAST of MC05g0155 vs. ExPASy TrEMBL
Match: A0A5D3D285 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002440 PE=3 SV=1)

HSP 1 Score: 862 bits (2228), Expect = 1.66e-315
Identity = 418/446 (93.72%), Postives = 433/446 (97.09%), Query Frame = 0

Query: 2   AMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEAL 61
           AMA SGKWIRL+LLV+FMLFVG IARVPKEI +T+NG +E LQSSSNS+MAVRLDE + L
Sbjct: 2   AMAESGKWIRLILLVMFMLFVGAIARVPKEIPETENGGSEKLQSSSNSTMAVRLDETKDL 61

Query: 62  DNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121
            N+HAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC
Sbjct: 62  GNEHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121

Query: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181
           GIGFGRNAIGGRDGRFYVVTDSGDDNPVNP PGTLRHAVIQDEPLWIVFKRDMVIQLKQE
Sbjct: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPKPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181

Query: 182 LIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241
           LIMNSFKTIDARG NVHIANGAC+TIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR
Sbjct: 182 LIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241

Query: 242 TMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSD 301
           T+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI++NYFTHHNEVMLLGHSD
Sbjct: 242 TIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNYFTHHNEVMLLGHSD 301

Query: 302 SYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361
           SYVRDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG
Sbjct: 302 SYVRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361

Query: 362 NRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSL 421
           NRYAAPTNPFAKEVTKRVETAE+EWK WNWRSEGDLLLNGAFFTPSGAGASASYARASSL
Sbjct: 362 NRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDLLLNGAFFTPSGAGASASYARASSL 421

Query: 422 GAKSSSMVASITSSAGALSCRRGHPC 447
           GAKSSSMV +ITS+AGALSCRRGHPC
Sbjct: 422 GAKSSSMVGTITSNAGALSCRRGHPC 447

BLAST of MC05g0155 vs. ExPASy TrEMBL
Match: A0A1S3AWX2 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103483506 PE=3 SV=1)

HSP 1 Score: 862 bits (2228), Expect = 1.66e-315
Identity = 418/446 (93.72%), Postives = 433/446 (97.09%), Query Frame = 0

Query: 2   AMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEAL 61
           AMA SGKWIRL+LLV+FMLFVG IARVPKEI +T+NG +E LQSSSNS+MAVRLDE + L
Sbjct: 2   AMAESGKWIRLILLVMFMLFVGAIARVPKEIPETENGGSEKLQSSSNSTMAVRLDETKDL 61

Query: 62  DNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121
            N+HAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC
Sbjct: 62  GNEHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121

Query: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181
           GIGFGRNAIGGRDGRFYVVTDSGDDNPVNP PGTLRHAVIQDEPLWIVFKRDMVIQLKQE
Sbjct: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPKPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181

Query: 182 LIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241
           LIMNSFKTIDARG NVHIANGAC+TIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR
Sbjct: 182 LIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241

Query: 242 TMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSD 301
           T+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI++NYFTHHNEVMLLGHSD
Sbjct: 242 TIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNYFTHHNEVMLLGHSD 301

Query: 302 SYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361
           SYVRDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG
Sbjct: 302 SYVRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361

Query: 362 NRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSL 421
           NRYAAPTNPFAKEVTKRVETAE+EWK WNWRSEGDLLLNGAFFTPSGAGASASYARASSL
Sbjct: 362 NRYAAPTNPFAKEVTKRVETAESEWKGWNWRSEGDLLLNGAFFTPSGAGASASYARASSL 421

Query: 422 GAKSSSMVASITSSAGALSCRRGHPC 447
           GAKSSSMV +ITS+AGALSCRRGHPC
Sbjct: 422 GAKSSSMVGTITSNAGALSCRRGHPC 447

BLAST of MC05g0155 vs. ExPASy TrEMBL
Match: A0A0A0L814 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_3G133180 PE=3 SV=1)

HSP 1 Score: 853 bits (2205), Expect = 5.07e-312
Identity = 414/446 (92.83%), Postives = 429/446 (96.19%), Query Frame = 0

Query: 2   AMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEAL 61
           AMA SGKWIRL+LLVVFMLFVG IAR PKEI + +NG TE LQSSSNS+MAVRLDE + L
Sbjct: 2   AMAESGKWIRLILLVVFMLFVGAIARAPKEIPEIENGGTEKLQSSSNSTMAVRLDEAKDL 61

Query: 62  DNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121
            N+HAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC
Sbjct: 62  GNEHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADC 121

Query: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181
           GIGFGRNAIGGRDGRFYVVTDSGDDNPVNP PGTLRHAVIQDEPLWIVFKRDMVIQLKQE
Sbjct: 122 GIGFGRNAIGGRDGRFYVVTDSGDDNPVNPKPGTLRHAVIQDEPLWIVFKRDMVIQLKQE 181

Query: 182 LIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241
           LIMNSFKTIDARG NVHIANGAC+TIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR
Sbjct: 182 LIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWR 241

Query: 242 TMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSD 301
           T+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI++NYFTHHNEVMLLGHSD
Sbjct: 242 TIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITISNNYFTHHNEVMLLGHSD 301

Query: 302 SYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361
           SYVRDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG
Sbjct: 302 SYVRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 361

Query: 362 NRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSL 421
           NRYAAPTNPFAKEVTKRVET+E+EWK WNWRSEGDLLLNGAFF PSGAGAS+SYARASSL
Sbjct: 362 NRYAAPTNPFAKEVTKRVETSESEWKGWNWRSEGDLLLNGAFFIPSGAGASSSYARASSL 421

Query: 422 GAKSSSMVASITSSAGALSCRRGHPC 447
           GAKSSSMV +ITS+AGALSCRRGH C
Sbjct: 422 GAKSSSMVGTITSNAGALSCRRGHAC 447

BLAST of MC05g0155 vs. ExPASy TrEMBL
Match: A0A6J1EAL4 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111432214 PE=3 SV=1)

HSP 1 Score: 852 bits (2201), Expect = 2.23e-311
Identity = 418/448 (93.30%), Postives = 432/448 (96.43%), Query Frame = 0

Query: 2   AMAASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEAL 61
           AMA SG WIRL LLVVFMLFVG IARVPKEI +  N AT  LQSSSNSSMAVRL+E EA 
Sbjct: 2   AMAESGGWIRLFLLVVFMLFVGAIARVPKEIPEIHNEATGKLQSSSNSSMAVRLEEPEAE 61

Query: 62  D--NKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLA 121
              NKHAVDNP+EIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLA
Sbjct: 62  TPGNKHAVDNPDEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLA 121

Query: 122 DCGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLK 181
           DCGIGFGRNAIGGRDGRFYV+TDSGDD+PVNP PGTLRHAVIQDEPLWIVFKRDMVIQLK
Sbjct: 122 DCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQDEPLWIVFKRDMVIQLK 181

Query: 182 QELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVG 241
           QELIMNSFKTIDARG+NVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVG
Sbjct: 182 QELIMNSFKTIDARGSNVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVG 241

Query: 242 WRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGH 301
           WRT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITIT+NYFTHHNEVMLLGH
Sbjct: 242 WRTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVMLLGH 301

Query: 302 SDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 361
           SDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS
Sbjct: 302 SDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINS 361

Query: 362 QGNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARAS 421
           QGNRYAAPTNPFAKEVTKRVETAE+EWK+WNWRSEGDLL+NGAFFTPSGAGASASYARAS
Sbjct: 362 QGNRYAAPTNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARAS 421

Query: 422 SLGAKSSSMVASITSSAGALSCRRGHPC 447
           SLGAKSSSMVA+IT++AGALSCRRGHPC
Sbjct: 422 SLGAKSSSMVATITATAGALSCRRGHPC 449

BLAST of MC05g0155 vs. TAIR 10
Match: AT4G13710.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 698.0 bits (1800), Expect = 5.0e-201
Identity = 343/449 (76.39%), Postives = 388/449 (86.41%), Query Frame = 0

Query: 4   AASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMAVRLDEVEALDN 63
           ++S K I + + V+ +L +  +     EIS ++   TE  QS ++S+MA    + E    
Sbjct: 3   SSSQKLISVCVAVLVVLALTAMIFRNSEISLSRKLKTEEFQSLNSSTMAATRLDGEPQQQ 62

Query: 64  KHAV-DNP----EEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRL 123
           +HAV D+P    +E+A +V MS +N TARR+LGFFSCGTGNPIDDCWRCD NW +NRKRL
Sbjct: 63  QHAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRL 122

Query: 124 ADCGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQL 183
           ADCGIGFGRNAIGGRDGRFY+VTD  D++ VNP PGTLRHAVIQ+EPLWIVFKRDMVI+L
Sbjct: 123 ADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIEL 182

Query: 184 KQELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHV 243
           KQELIMNSFKTIDARG+NVHIANGACITIQF+TNVI+HGL+IHDCKPTGNAMVRSSP+H 
Sbjct: 183 KQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHF 242

Query: 244 GWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLG 303
           GWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMGSTAIT+++N+FTHHNEVMLLG
Sbjct: 243 GWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLLG 302

Query: 304 HSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 363
           HSDSY +DK MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTIN
Sbjct: 303 HSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTIN 362

Query: 364 SQGNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARA 423
           SQGNRYAAP + FAKEVTKRVET  +EWK WNWRSEGDLLLNGAFF PSGAGASASY RA
Sbjct: 363 SQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRA 422

Query: 424 SSLGAKSSSMVASITSSAGALSCRRGHPC 448
           SSL AK SSMV +ITS+AGAL CR+G PC
Sbjct: 423 SSLAAKPSSMVDTITSTAGALGCRKGRPC 451

BLAST of MC05g0155 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 695.3 bits (1793), Expect = 3.3e-200
Identity = 345/468 (73.72%), Postives = 398/468 (85.04%), Query Frame = 0

Query: 4   AASGKWIRLVLLVVFMLFVGTIARVPKEISQTQNGATEMLQSSSNSSMA----VRLDEVE 63
           ++S K I + + V+ +L +  +     EIS ++   TE++QSS++S+MA    ++ +E +
Sbjct: 3   SSSQKLISVCVAVLVVLALTAMIFRNSEISLSRKLKTEVIQSSNSSTMAAIRKLKTEEFQ 62

Query: 64  ALDN---------------KHAV-DNP----EEIAAMVDMSIRNATARRELGFFSCGTGN 123
           +L++               +HAV D+P    +E+A +V MS +N TARR+LGFFSCGTGN
Sbjct: 63  SLNSSTMAATRLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFSCGTGN 122

Query: 124 PIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHA 183
           PIDDCWRCD NW +NRKRLADCGIGFGRNAIGGRDGRFY+VTD  D++ VNP PGTLRHA
Sbjct: 123 PIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHA 182

Query: 184 VIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLN 243
           VIQ+EPLWIVFKRDMVI+LKQELIMNSFKTIDARG+NVHIANGACITIQF+TNVI+HGL+
Sbjct: 183 VIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLH 242

Query: 244 IHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGST 303
           IHDCKPTGNAMVRSSP+H GWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDAVMGST
Sbjct: 243 IHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGST 302

Query: 304 AITITSNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNN 363
           AIT+++N+FTHHNEVMLLGHSDSY +DK MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNN
Sbjct: 303 AITVSNNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNN 362

Query: 364 DYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLL 423
           DYTHWEMYAIGGSA PTINSQGNRYAAP + FAKEVTKRVET  +EWK WNWRSEGDLLL
Sbjct: 363 DYTHWEMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLL 422

Query: 424 NGAFFTPSGAGASASYARASSLGAKSSSMVASITSSAGALSCRRGHPC 448
           NGAFF PSGAGASASY RASSL AK SSMV +ITS+AGAL CR+G PC
Sbjct: 423 NGAFFRPSGAGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

BLAST of MC05g0155 vs. TAIR 10
Match: AT1G04680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 694.1 bits (1790), Expect = 7.3e-200
Identity = 336/404 (83.17%), Postives = 358/404 (88.61%), Query Frame = 0

Query: 48  NSSMAVRLDEVEALDNKHAVDNPEEIA----AMVDMSIRNATARRELGFFSCGTGNPIDD 107
           N S   R DE E   N+HAV NP+E+A    A+ +MS+RN T RR+LG+F+CGTGNPIDD
Sbjct: 28  NISSLPRSDETEW--NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDD 87

Query: 108 CWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQD 167
           CWRCDPNW +NRKRLADCGIGFGRNAIGGRDGRFYVVTD  DDNPVNP PGTLRHAVIQD
Sbjct: 88  CWRCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQD 147

Query: 168 EPLWIVFKRDMVIQLKQELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDC 227
            PLWIVFKRDMVIQLKQELI+NSFKTID RGANVHIANG CITIQFVTNVIVHGL+IHDC
Sbjct: 148 RPLWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDC 207

Query: 228 KPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI 287
           KPTGNAMVRSS  H GWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITI
Sbjct: 208 KPTGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITI 267

Query: 288 TSNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTH 347
           ++N+ THHNEVMLLGHSDSY+RDK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTH
Sbjct: 268 SNNHLTHHNEVMLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTH 327

Query: 348 WEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAF 407
           WEMYAIGGSANPTINSQGNRYAAP NPFAKEVTKRV+T  + WK WNWRSEGDLL NGA+
Sbjct: 328 WEMYAIGGSANPTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAY 387

Query: 408 FTPSGAGASASYARASSLGAKSSSMVASITSSAGALSCRRGHPC 448
           FT SGA AS SYARASSL AKSSS+V  ITS AGAL CRRG  C
Sbjct: 388 FTSSGAAASGSYARASSLSAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of MC05g0155 vs. TAIR 10
Match: AT3G07010.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 685.6 bits (1768), Expect = 2.6e-197
Identity = 321/385 (83.38%), Postives = 353/385 (91.69%), Query Frame = 0

Query: 63  NKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122
           ++HAV+NP+E+AAMVDMSIRN+T RR LG+FSC TGNPIDDCWRCD  WQ  RKRLADC 
Sbjct: 32  HEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCS 91

Query: 123 IGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 182
           IGFGRNAIGGRDGRFYVVTD GDD+PVNP+PGTLRHAVIQDEPLWI+FKRDMVI LKQEL
Sbjct: 92  IGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQEL 151

Query: 183 IMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242
           IMNSFKTID RG NVHIANGAC+TIQ+VTN+IVHG+++HDCKPTGNAMVRSSP+H G+R+
Sbjct: 152 IMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRS 211

Query: 243 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSDS 302
           MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT+++N+FTHHNEVMLLGHSDS
Sbjct: 212 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLLGHSDS 271

Query: 303 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGN
Sbjct: 272 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTINSQGN 331

Query: 363 RYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSLG 422
           R+ AP NPFAKEVTKR  T E++WK WNWRSEGDL LNGAFFT SGAGA A+YARASSL 
Sbjct: 332 RFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYARASSLS 391

Query: 423 AKSSSMVASITSSAGALSCRRGHPC 448
           AKSSS+V ++TS +GAL+CR G  C
Sbjct: 392 AKSSSLVGTMTSYSGALNCRAGRRC 416

BLAST of MC05g0155 vs. TAIR 10
Match: AT5G48900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 674.9 bits (1740), Expect = 4.6e-194
Identity = 317/385 (82.34%), Postives = 346/385 (89.87%), Query Frame = 0

Query: 63  NKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 122
           ++HAV+NPEE+AAMVDMSIRN+TARR LG+FSC TGNPIDDCWRCD  WQ  RK LA+C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 123 IGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 182
           IGFGRNAIGGRDGR+YVV+D  DDNPVNP PGTLRHAVIQ+EPLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 183 IMNSFKTIDARGANVHIANGACITIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 242
           IMNSFKTID RG NVHIANGACITIQFVTN+I+HG++IHDC+PTGNAMVRSSP+H GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 243 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITSNYFTHHNEVMLLGHSDS 302
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITI++NYFTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 303 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 362
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 363 RYAAPTNPFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSLG 422
           RY AP N FAKEVTKR    + +W+ WNWRSEGDL LNGAFFT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 423 AKSSSMVASITSSAGALSCRRGHPC 448
           AKSSS+V  IT +AGAL+CR G  C
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417