MC05g0083 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0083
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	ethylene-responsive transcription factor 1B
Location	MC05: 590752 .. 591405 (+)
RNA-Seq Expression	MC05g0083
Synteny	MC05g0083

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCTCCGGCTTCTTCTCCCCGGCTTCCGATTTCTCATCGGAATCTTCCTTCGGTTCGCCGGAATCCTCCTTCATGAATTTGGACCACTATTTTCTCCCTTTCAACGAGAACGATTCGGAAGAAATGCTTCTCTACGAACTGATCTCGGAGGGGACTCAGGGGGGCGTTCGAGTGAAGGAAGAGGAAGTCGATTCCGTCGGCGAGGAGAGCCCCAAGAAGGAGAGATCTTACCGGGGCGTTCGCCGGCGGCCGTGGGGCAAATTTGCGGCGGAGATTAGAGATTCCACCCGACATGGAATCAGGGTCTGGTTGGGGACTTTCGACAGTGCTGAGGCAGCCGCTCTGGCCTACGATCAAGCCGCGTTTTCGATGAGGGGGGCCGCGGCAATTCTCAATTTTCCGGTGGACAGAGTTAGGGAGTCTCTGAAAGAAATGAACTGCAGCGGCGGCGGCGGCGGCGGCGGCGATGACGGCGGGTCTCCGGTGGTGGCGTTGAAAAGAAAACACTCCATCAGAAGGAAGGCCATAAGCAAAAAGAGCAAAGAAAGAGATGTGAGGATCCAAAATGTGGTGGTTTTGGAAGATTTAGGGGCAGAGTATTTGGAAGAACTTTTGGGCTCTTCTCAAAGTGGTAGCCCTTGTTCTTAC

mRNA sequence

ATGGATTCCTCCGGCTTCTTCTCCCCGGCTTCCGATTTCTCATCGGAATCTTCCTTCGGTTCGCCGGAATCCTCCTTCATGAATTTGGACCACTATTTTCTCCCTTTCAACGAGAACGATTCGGAAGAAATGCTTCTCTACGAACTGATCTCGGAGGGGACTCAGGGGGGCGTTCGAGTGAAGGAAGAGGAAGTCGATTCCGTCGGCGAGGAGAGCCCCAAGAAGGAGAGATCTTACCGGGGCGTTCGCCGGCGGCCGTGGGGCAAATTTGCGGCGGAGATTAGAGATTCCACCCGACATGGAATCAGGGTCTGGTTGGGGACTTTCGACAGTGCTGAGGCAGCCGCTCTGGCCTACGATCAAGCCGCGTTTTCGATGAGGGGGGCCGCGGCAATTCTCAATTTTCCGGTGGACAGAGTTAGGGAGTCTCTGAAAGAAATGAACTGCAGCGGCGGCGGCGGCGGCGGCGGCGATGACGGCGGGTCTCCGGTGGTGGCGTTGAAAAGAAAACACTCCATCAGAAGGAAGGCCATAAGCAAAAAGAGCAAAGAAAGAGATGTGAGGATCCAAAATGTGGTGGTTTTGGAAGATTTAGGGGCAGAGTATTTGGAAGAACTTTTGGGCTCTTCTCAAAGTGGTAGCCCTTGTTCTTAC

Coding sequence (CDS)

ATGGATTCCTCCGGCTTCTTCTCCCCGGCTTCCGATTTCTCATCGGAATCTTCCTTCGGTTCGCCGGAATCCTCCTTCATGAATTTGGACCACTATTTTCTCCCTTTCAACGAGAACGATTCGGAAGAAATGCTTCTCTACGAACTGATCTCGGAGGGGACTCAGGGGGGCGTTCGAGTGAAGGAAGAGGAAGTCGATTCCGTCGGCGAGGAGAGCCCCAAGAAGGAGAGATCTTACCGGGGCGTTCGCCGGCGGCCGTGGGGCAAATTTGCGGCGGAGATTAGAGATTCCACCCGACATGGAATCAGGGTCTGGTTGGGGACTTTCGACAGTGCTGAGGCAGCCGCTCTGGCCTACGATCAAGCCGCGTTTTCGATGAGGGGGGCCGCGGCAATTCTCAATTTTCCGGTGGACAGAGTTAGGGAGTCTCTGAAAGAAATGAACTGCAGCGGCGGCGGCGGCGGCGGCGGCGATGACGGCGGGTCTCCGGTGGTGGCGTTGAAAAGAAAACACTCCATCAGAAGGAAGGCCATAAGCAAAAAGAGCAAAGAAAGAGATGTGAGGATCCAAAATGTGGTGGTTTTGGAAGATTTAGGGGCAGAGTATTTGGAAGAACTTTTGGGCTCTTCTCAAAGTGGTAGCCCTTGTTCTTAC

Protein sequence

MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQGGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEAAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIRRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCSY

Homology

[Close]

BLAST of MC05g0083 vs. ExPASy Swiss-Prot
Match: Q8LDC8 (Ethylene-responsive transcription factor 1B OS=Arabidopsis thaliana OX=3702 GN=ERF1B PE=1 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 4.7e-56
Identity = 132/226 (58.41%), Postives = 160/226 (70.80%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSF--MNLDHYFLPFNENDSEEMLLYELISEGTQGGV 60
           MD     SP S FS E S GS   SF   + ++Y LPFNENDSEEM LY LI + TQ   
Sbjct: 1   MDPFLIQSPFSGFSPEYSIGSSPDSFSSSSSNNYSLPFNENDSEEMFLYGLIEQSTQ--- 60

Query: 61  RVKEEEVDSVGEESP-------KKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDS 120
              +  +DS  ++ P       K E+SYRGVRRRPWGKFAAEIRDSTR+GIRVWLGTF+S
Sbjct: 61  ---QTYIDSDSQDLPIKSVSSRKSEKSYRGVRRRPWGKFAAEIRDSTRNGIRVWLGTFES 120

Query: 121 AEAAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKH 180
           AE AALAYDQAAFSMRG++AILNF  +RV+ESL E+  +       +DG SPVVALKRKH
Sbjct: 121 AEEAALAYDQAAFSMRGSSAILNFSAERVQESLSEIKYT------YEDGCSPVVALKRKH 180

Query: 181 SIRRKAISKKSKE-----RDVRIQNVVVLEDLGAEYLEELLGSSQS 213
           S+RR+  +KK+K+     R V++ NVVV EDLG +YLEELLGSS++
Sbjct: 181 SMRRRMTNKKTKDSDFDHRSVKLDNVVVFEDLGEQYLEELLGSSEN 214

[Close]

BLAST of MC05g0083 vs. ExPASy Swiss-Prot
Match: A0A3Q7I5Y9 (Ethylene-response factor C3 OS=Solanum lycopersicum OX=4081 GN=ERF.C.3 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 4.6e-51
Identity = 113/192 (58.85%), Postives = 146/192 (76.04%), Query Frame = 0


Query: 29  LDHY-FLPFNENDSEEMLLYELISEG-------TQGGVRVKEEEVDSVGEESPKKERSYR 88
           L HY  LPFN ND+++MLLY L++EG       +     +KEEEV S  EE  +K+++YR
Sbjct: 10  LFHYNSLPFNVNDTQDMLLYNLVAEGSSQETVNSSSSYGIKEEEVTSYEEE--RKDKNYR 69

Query: 89  GVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEAAALAYDQAAFSMRGAAAILNFPVDRV 148
           GVR+RPWGK+AAEIRDSTR+G+RVWLGTFD+AE AALAYDQAAF+MRG+ AILNFPV+ V
Sbjct: 70  GVRKRPWGKYAAEIRDSTRNGVRVWLGTFDNAEEAALAYDQAAFAMRGSMAILNFPVEIV 129

Query: 149 RESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIRRKAISKKSKERDVRIQNVVVLEDLGA 208
           +ESL EM C        D   SPV+ LK+++S+RRK++S+K++ R    ++VVV EDLGA
Sbjct: 130 KESLNEMKCR------FDGNCSPVIELKKRYSMRRKSVSRKNRAR----KDVVVFEDLGA 189

Query: 209 EYLEELLGSSQS 213
           EYLEELL SS+S
Sbjct: 190 EYLEELLISSES 189

[Close]

BLAST of MC05g0083 vs. ExPASy Swiss-Prot
Match: Q8VYM0 (Ethylene-responsive transcription factor 15 OS=Arabidopsis thaliana OX=3702 GN=ERF15 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.9e-39
Identity = 117/246 (47.56%), Postives = 147/246 (59.76%), Query Frame = 0


Query: 3   SSGFFSPASDFSSESSFGSPESSFMNLDH----YFLPFNENDSEEMLLYE----LISEGT 62
           SS + SP+S  SS+ S    ES F++       +F P N + S++   +E    +I E  
Sbjct: 6   SSMYSSPSSWSSSQESLLWNESCFLDQSSEPQAFFCP-NYDYSDDFFSFESPEMMIKEEI 65

Query: 63  QGGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEA 122
           Q G     EE + VG +   +ERSYRGVR+RPWGKFAAEIRDSTR+GIRVWLGTFD AE 
Sbjct: 66  QNGDVSNSEEEEKVGID---EERSYRGVRKRPWGKFAAEIRDSTRNGIRVWLGTFDKAEE 125

Query: 123 AALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIR 182
           AALAYDQAAF+ +G+ A LNFPV+ VRESLK+M           DGGSPV+ALKRKHS+R
Sbjct: 126 AALAYDQAAFATKGSLATLNFPVEVVRESLKKME-----NVNLHDGGSPVMALKRKHSLR 185

Query: 183 RKAISKK----------------------------SKERDVRIQN--VVVLEDLGAEYLE 211
            +   KK                            SK+  V+ ++  +VV EDLGAEYLE
Sbjct: 186 NRPRGKKRSSSSSSSSSNSSSCSSSSSTSSTSRSSSKQSVVKQESGTLVVFEDLGAEYLE 242

[Close]

BLAST of MC05g0083 vs. ExPASy Swiss-Prot
Match: Q9LND1 (Ethylene-responsive transcription factor ERF094 OS=Arabidopsis thaliana OX=3702 GN=ERF094 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.2e-36
Identity = 112/253 (44.27%), Postives = 147/253 (58.10%), Query Frame = 0


Query: 4   SGFFSPASDFSSESSFGSPES-----SFM--NLDHYFLPFNENDS--EEMLLYE---LIS 63
           SG FSP +  SS SS+ S ES     SF+  + D  FL  +  D+  ++   +E   +  
Sbjct: 9   SGEFSPEN--SSSSSWSSQESFLWEESFLHQSFDQSFLLSSPTDNYCDDFFAFESSIIKE 68

Query: 64  EGTQGGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDS 123
           EG +  V  +EE            E+SYRGVR+RPWGKFAAEIRDSTR GIRVWLGTFD+
Sbjct: 69  EGKEATVAAEEE------------EKSYRGVRKRPWGKFAAEIRDSTRKGIRVWLGTFDT 128

Query: 124 AEAAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKH 183
           AEAAALAYDQAAF+++G+ A+LNFP D V ESL++M          +DG SPV+ALKRKH
Sbjct: 129 AEAAALAYDQAAFALKGSLAVLNFPADVVEESLRKME-----NVNLNDGESPVIALKRKH 188

Query: 184 SIRRK------------------------------AISKKSKERDVRI-----QNVVVLE 210
           S+R +                              ++S +S+++ V +       +VVLE
Sbjct: 189 SMRNRPRGKKKSSSSSTLTSSPSSSSSYSSSSSSSSLSSRSRKQSVVMTQESNTTLVVLE 242

[Close]

BLAST of MC05g0083 vs. ExPASy Swiss-Prot
Match: O04681 (Pathogenesis-related genes transcriptional activator PTI5 OS=Solanum lycopersicum OX=4081 GN=PTI5 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 7.8e-27
Identity = 63/116 (54.31%), Postives = 81/116 (69.83%), Query Frame = 0


Query: 34  LPFNENDSEEMLLYELISEGTQGGVRVKEEEVDSVGEESPKKE-----RSYRGVRRRPWG 93
           LP NENDS+EM+LYE+++E     +    +    +   +  +      + YRGVRRRPWG
Sbjct: 9   LPLNENDSQEMVLYEVLNEANALNIPYLPQRNQLLPRNNILRPLQCIGKKYRGVRRRPWG 68

Query: 94  KFAAEIRDSTRHGIRVWLGTFDSAEAAALAYDQAAFSMRGAAAILNFPVDRVRESL 145
           K+AAEIRDS RHG RVWLGTF++AE AALAYD+AAF MRGA A+LNFP + V  S+
Sbjct: 69  KYAAEIRDSARHGARVWLGTFETAEEAALAYDRAAFRMRGAKALLNFPSEIVNASV 124

[Close]

BLAST of MC05g0083 vs. NCBI nr
Match: XP_022150897.1 (ethylene-responsive transcription factor 1B [Momordica charantia])

HSP 1 Score: 415 bits (1066), Expect = 8.25e-146
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQGGVRV 60
           MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQGGVRV
Sbjct: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQGGVRV 60

Query: 61  KEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEAAALAYD 120
           KEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEAAALAYD
Sbjct: 61  KEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEAAALAYD 120

Query: 121 QAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIRRKAISK 180
           QAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIRRKAISK
Sbjct: 121 QAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIRRKAISK 180

Query: 181 KSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCSY 218
           KSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCSY
Sbjct: 181 KSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCSY 218

[Close]

BLAST of MC05g0083 vs. NCBI nr
Match: XP_022975053.1 (ethylene-responsive transcription factor 1B-like [Cucurbita maxima])

HSP 1 Score: 363 bits (933), Expect = 1.97e-125
Identity = 196/223 (87.89%), Postives = 202/223 (90.58%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQ----- 60
           MD SGFFSPASDFSSESSF SPESSF+NLDH FLPFNENDSEEMLLYELISEG Q     
Sbjct: 1   MDYSGFFSPASDFSSESSFDSPESSFINLDHNFLPFNENDSEEMLLYELISEGRQDSSEK 60

Query: 61  --GGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120
             G VRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE
Sbjct: 61  GIGAVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120

Query: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSI 180
           AAALAYDQAAF+MRGAAAILNFPVDRVRESLKEMNCSG G G  +DGGSPV+ALKRKHS+
Sbjct: 121 AAALAYDQAAFAMRGAAAILNFPVDRVRESLKEMNCSGDGYGLAEDGGSPVIALKRKHSV 180

Query: 181 RRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPC 216
           RR  ISKK KERDVRIQN+VVLEDLG EYLEELLG SQSGSPC
Sbjct: 181 RRNGISKKKKERDVRIQNMVVLEDLGTEYLEELLGCSQSGSPC 223

[Close]

BLAST of MC05g0083 vs. NCBI nr
Match: XP_038879960.1 (ethylene-response factor C3-like [Benincasa hispida])

HSP 1 Score: 362 bits (929), Expect = 8.60e-125
Identity = 199/227 (87.67%), Postives = 204/227 (89.87%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQGG--- 60
           MD SG FS  SDFSSESSFGSPESSFMNLDH FLPFNENDSEEMLLY LIS GTQ     
Sbjct: 1   MDYSGLFSSISDFSSESSFGSPESSFMNLDHDFLPFNENDSEEMLLYGLISGGTQDSSDT 60

Query: 61  ----VRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120
               VRVKEEEVDS+GEESPKKER+YRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE
Sbjct: 61  GIRTVRVKEEEVDSIGEESPKKERAYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120

Query: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGG--DDGGSPVVALKRKH 180
           AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGG  G    +DGGSPVVALKRKH
Sbjct: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGAAGDNMAEDGGSPVVALKRKH 180

Query: 181 SIRRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCSY 218
           SIRRKAISKKSKERDVRIQNV+VLEDLG EYLEELLGSSQSGSPCS+
Sbjct: 181 SIRRKAISKKSKERDVRIQNVMVLEDLGTEYLEELLGSSQSGSPCSF 227

[Close]

BLAST of MC05g0083 vs. NCBI nr
Match: KAG6597369.1 (Ethylene-responsive transcription factor 1B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 360 bits (923), Expect = 6.58e-124
Identity = 195/224 (87.05%), Postives = 200/224 (89.29%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQ----- 60
           MD SGFFSPASDFSSESSF SPESSF+NLDH FLPFNENDSEEMLLYELISEG       
Sbjct: 1   MDYSGFFSPASDFSSESSFDSPESSFINLDHDFLPFNENDSEEMLLYELISEGRNDSSEK 60

Query: 61  --GGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120
             G VRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTR GIRVWLGTFDSAE
Sbjct: 61  GIGAVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRRGIRVWLGTFDSAE 120

Query: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSI 180
           AAALAYDQAAFSMRGA AILNFPVDRVRESLKEMNC G G G  +DGGSPV+ALKRKHS+
Sbjct: 121 AAALAYDQAAFSMRGATAILNFPVDRVRESLKEMNCGGDGYGLAEDGGSPVIALKRKHSV 180

Query: 181 RRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCS 217
           RR  ISKK KERDVRIQN+VVLEDLG EYLEELLGSSQSGSPCS
Sbjct: 181 RRNGISKKKKERDVRIQNMVVLEDLGTEYLEELLGSSQSGSPCS 224

[Close]

BLAST of MC05g0083 vs. NCBI nr
Match: XP_022942451.1 (ethylene-responsive transcription factor 1B-like [Cucurbita moschata])

HSP 1 Score: 356 bits (914), Expect = 1.55e-122
Identity = 193/224 (86.16%), Postives = 199/224 (88.84%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQ----- 60
           MD SGFFSPASDFSSESSF SPESSF+NLDH FLPFNENDSEEMLLYELISEG Q     
Sbjct: 1   MDYSGFFSPASDFSSESSFDSPESSFINLDHDFLPFNENDSEEMLLYELISEGRQDSSEK 60

Query: 61  --GGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120
             G VRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTR GIRVWLGTFDSAE
Sbjct: 61  GIGAVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRRGIRVWLGTFDSAE 120

Query: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSI 180
           AAALAYDQAAF+MRGA AILNFPVDRVRESLKEMNC G G G  +DGGSPV+ALKRKHS+
Sbjct: 121 AAALAYDQAAFAMRGATAILNFPVDRVRESLKEMNCGGDGYGLAEDGGSPVIALKRKHSV 180

Query: 181 RRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCS 217
           RR  ISK  KERDVRIQN+VVLEDLG EYLEELLG SQSGSPCS
Sbjct: 181 RRNGISKMKKERDVRIQNMVVLEDLGTEYLEELLGCSQSGSPCS 224

[Close]

BLAST of MC05g0083 vs. ExPASy TrEMBL
Match: A0A6J1DC08 (ethylene-responsive transcription factor 1B OS=Momordica charantia OX=3673 GN=LOC111018935 PE=4 SV=1)

HSP 1 Score: 415 bits (1066), Expect = 3.99e-146
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQGGVRV 60
           MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQGGVRV
Sbjct: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQGGVRV 60

Query: 61  KEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEAAALAYD 120
           KEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEAAALAYD
Sbjct: 61  KEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEAAALAYD 120

Query: 121 QAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIRRKAISK 180
           QAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIRRKAISK
Sbjct: 121 QAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIRRKAISK 180

Query: 181 KSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCSY 218
           KSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCSY
Sbjct: 181 KSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCSY 218

[Close]

BLAST of MC05g0083 vs. ExPASy TrEMBL
Match: A0A6J1II54 (ethylene-responsive transcription factor 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111474018 PE=4 SV=1)

HSP 1 Score: 363 bits (933), Expect = 9.54e-126
Identity = 196/223 (87.89%), Postives = 202/223 (90.58%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQ----- 60
           MD SGFFSPASDFSSESSF SPESSF+NLDH FLPFNENDSEEMLLYELISEG Q     
Sbjct: 1   MDYSGFFSPASDFSSESSFDSPESSFINLDHNFLPFNENDSEEMLLYELISEGRQDSSEK 60

Query: 61  --GGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120
             G VRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE
Sbjct: 61  GIGAVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120

Query: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSI 180
           AAALAYDQAAF+MRGAAAILNFPVDRVRESLKEMNCSG G G  +DGGSPV+ALKRKHS+
Sbjct: 121 AAALAYDQAAFAMRGAAAILNFPVDRVRESLKEMNCSGDGYGLAEDGGSPVIALKRKHSV 180

Query: 181 RRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPC 216
           RR  ISKK KERDVRIQN+VVLEDLG EYLEELLG SQSGSPC
Sbjct: 181 RRNGISKKKKERDVRIQNMVVLEDLGTEYLEELLGCSQSGSPC 223

[Close]

BLAST of MC05g0083 vs. ExPASy TrEMBL
Match: A0A6J1FNW6 (ethylene-responsive transcription factor 1B-like OS=Cucurbita moschata OX=3662 GN=LOC111447483 PE=4 SV=1)

HSP 1 Score: 356 bits (914), Expect = 7.48e-123
Identity = 193/224 (86.16%), Postives = 199/224 (88.84%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQ----- 60
           MD SGFFSPASDFSSESSF SPESSF+NLDH FLPFNENDSEEMLLYELISEG Q     
Sbjct: 1   MDYSGFFSPASDFSSESSFDSPESSFINLDHDFLPFNENDSEEMLLYELISEGRQDSSEK 60

Query: 61  --GGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120
             G VRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTR GIRVWLGTFDSAE
Sbjct: 61  GIGAVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRRGIRVWLGTFDSAE 120

Query: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSI 180
           AAALAYDQAAF+MRGA AILNFPVDRVRESLKEMNC G G G  +DGGSPV+ALKRKHS+
Sbjct: 121 AAALAYDQAAFAMRGATAILNFPVDRVRESLKEMNCGGDGYGLAEDGGSPVIALKRKHSV 180

Query: 181 RRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSPCS 217
           RR  ISK  KERDVRIQN+VVLEDLG EYLEELLG SQSGSPCS
Sbjct: 181 RRNGISKMKKERDVRIQNMVVLEDLGTEYLEELLGCSQSGSPCS 224

[Close]

BLAST of MC05g0083 vs. ExPASy TrEMBL
Match: A0A5A7U4J3 (Ethylene-responsive transcription factor 1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001540 PE=4 SV=1)

HSP 1 Score: 352 bits (902), Expect = 5.59e-121
Identity = 197/228 (86.40%), Postives = 203/228 (89.04%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQ----- 60
           MD S FFSP SDFSSESSFGSPESSF NLDH FLPFNENDSEEMLLY LISEGT      
Sbjct: 1   MDYSVFFSPVSDFSSESSFGSPESSFTNLDHNFLPFNENDSEEMLLYGLISEGTYESSDT 60

Query: 61  --GGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120
             G V+VKEEEVDS+GEESPKKER+YRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE
Sbjct: 61  SIGTVQVKEEEVDSIGEESPKKERAYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120

Query: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGG--DDGGSPVVALKRKH 180
           AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMN   GG G    +DGGSPVVALKRKH
Sbjct: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNGGSGGSGNSLAEDGGSPVVALKRKH 180

Query: 181 SIRRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSP-CSY 218
           SIRRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQS +P CS+
Sbjct: 181 SIRRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSDNPSCSF 228

[Close]

BLAST of MC05g0083 vs. ExPASy TrEMBL
Match: A0A1S4DSE7 (ethylene-responsive transcription factor 1B OS=Cucumis melo OX=3656 GN=LOC103483599 PE=4 SV=1)

HSP 1 Score: 352 bits (902), Expect = 5.59e-121
Identity = 197/228 (86.40%), Postives = 203/228 (89.04%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSFMNLDHYFLPFNENDSEEMLLYELISEGTQ----- 60
           MD S FFSP SDFSSESSFGSPESSF NLDH FLPFNENDSEEMLLY LISEGT      
Sbjct: 1   MDYSVFFSPVSDFSSESSFGSPESSFTNLDHNFLPFNENDSEEMLLYGLISEGTYESSDT 60

Query: 61  --GGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120
             G V+VKEEEVDS+GEESPKKER+YRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE
Sbjct: 61  SIGTVQVKEEEVDSIGEESPKKERAYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAE 120

Query: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGG--DDGGSPVVALKRKH 180
           AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMN   GG G    +DGGSPVVALKRKH
Sbjct: 121 AAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNGGSGGSGNSLAEDGGSPVVALKRKH 180

Query: 181 SIRRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSGSP-CSY 218
           SIRRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQS +P CS+
Sbjct: 181 SIRRKAISKKSKERDVRIQNVVVLEDLGAEYLEELLGSSQSDNPSCSF 228

[Close]

BLAST of MC05g0083 vs. TAIR 10
Match: AT3G23240.1 (ethylene response factor 1 )

HSP 1 Score: 219.2 bits (557), Expect = 3.3e-57
Identity = 132/226 (58.41%), Postives = 160/226 (70.80%), Query Frame = 0


Query: 1   MDSSGFFSPASDFSSESSFGSPESSF--MNLDHYFLPFNENDSEEMLLYELISEGTQGGV 60
           MD     SP S FS E S GS   SF   + ++Y LPFNENDSEEM LY LI + TQ   
Sbjct: 1   MDPFLIQSPFSGFSPEYSIGSSPDSFSSSSSNNYSLPFNENDSEEMFLYGLIEQSTQ--- 60

Query: 61  RVKEEEVDSVGEESP-------KKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDS 120
              +  +DS  ++ P       K E+SYRGVRRRPWGKFAAEIRDSTR+GIRVWLGTF+S
Sbjct: 61  ---QTYIDSDSQDLPIKSVSSRKSEKSYRGVRRRPWGKFAAEIRDSTRNGIRVWLGTFES 120

Query: 121 AEAAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKH 180
           AE AALAYDQAAFSMRG++AILNF  +RV+ESL E+  +       +DG SPVVALKRKH
Sbjct: 121 AEEAALAYDQAAFSMRGSSAILNFSAERVQESLSEIKYT------YEDGCSPVVALKRKH 180

Query: 181 SIRRKAISKKSKE-----RDVRIQNVVVLEDLGAEYLEELLGSSQS 213
           S+RR+  +KK+K+     R V++ NVVV EDLG +YLEELLGSS++
Sbjct: 181 SMRRRMTNKKTKDSDFDHRSVKLDNVVVFEDLGEQYLEELLGSSEN 214

[Close]

BLAST of MC05g0083 vs. TAIR 10
Match: AT2G31230.1 (ethylene-responsive element binding factor 15 )

HSP 1 Score: 161.8 bits (408), Expect = 6.3e-40
Identity = 117/246 (47.56%), Postives = 147/246 (59.76%), Query Frame = 0


Query: 3   SSGFFSPASDFSSESSFGSPESSFMNLDH----YFLPFNENDSEEMLLYE----LISEGT 62
           SS + SP+S  SS+ S    ES F++       +F P N + S++   +E    +I E  
Sbjct: 6   SSMYSSPSSWSSSQESLLWNESCFLDQSSEPQAFFCP-NYDYSDDFFSFESPEMMIKEEI 65

Query: 63  QGGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEA 122
           Q G     EE + VG +   +ERSYRGVR+RPWGKFAAEIRDSTR+GIRVWLGTFD AE 
Sbjct: 66  QNGDVSNSEEEEKVGID---EERSYRGVRKRPWGKFAAEIRDSTRNGIRVWLGTFDKAEE 125

Query: 123 AALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIR 182
           AALAYDQAAF+ +G+ A LNFPV+ VRESLK+M           DGGSPV+ALKRKHS+R
Sbjct: 126 AALAYDQAAFATKGSLATLNFPVEVVRESLKKME-----NVNLHDGGSPVMALKRKHSLR 185

Query: 183 RKAISKK----------------------------SKERDVRIQN--VVVLEDLGAEYLE 211
            +   KK                            SK+  V+ ++  +VV EDLGAEYLE
Sbjct: 186 NRPRGKKRSSSSSSSSSNSSSCSSSSSTSSTSRSSSKQSVVKQESGTLVVFEDLGAEYLE 242

[Close]

BLAST of MC05g0083 vs. TAIR 10
Match: AT1G06160.1 (octadecanoid-responsive Arabidopsis AP2/ERF 59 )

HSP 1 Score: 153.3 bits (386), Expect = 2.3e-37
Identity = 112/253 (44.27%), Postives = 147/253 (58.10%), Query Frame = 0


Query: 4   SGFFSPASDFSSESSFGSPES-----SFM--NLDHYFLPFNENDS--EEMLLYE---LIS 63
           SG FSP +  SS SS+ S ES     SF+  + D  FL  +  D+  ++   +E   +  
Sbjct: 9   SGEFSPEN--SSSSSWSSQESFLWEESFLHQSFDQSFLLSSPTDNYCDDFFAFESSIIKE 68

Query: 64  EGTQGGVRVKEEEVDSVGEESPKKERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDS 123
           EG +  V  +EE            E+SYRGVR+RPWGKFAAEIRDSTR GIRVWLGTFD+
Sbjct: 69  EGKEATVAAEEE------------EKSYRGVRKRPWGKFAAEIRDSTRKGIRVWLGTFDT 128

Query: 124 AEAAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKH 183
           AEAAALAYDQAAF+++G+ A+LNFP D V ESL++M          +DG SPV+ALKRKH
Sbjct: 129 AEAAALAYDQAAFALKGSLAVLNFPADVVEESLRKME-----NVNLNDGESPVIALKRKH 188

Query: 184 SIRRK------------------------------AISKKSKERDVRI-----QNVVVLE 210
           S+R +                              ++S +S+++ V +       +VVLE
Sbjct: 189 SMRNRPRGKKKSSSSSTLTSSPSSSSSYSSSSSSSSLSSRSRKQSVVMTQESNTTLVVLE 242

[Close]

BLAST of MC05g0083 vs. TAIR 10
Match: AT4G17500.1 (ethylene responsive element binding factor 1 )

HSP 1 Score: 109.4 bits (272), Expect = 3.7e-24
Identity = 70/162 (43.21%), Postives = 91/162 (56.17%), Query Frame = 0


Query: 34  LPFNENDSEEMLLYELISEGTQGG-----------------VRVKEEE----VDSVGEES 93
           LP  ENDSE+ML+Y ++++   GG                 V+++  E    VDSV  + 
Sbjct: 71  LPLKENDSEDMLVYGILNDAFHGGWEPSSSSSDEDRSSFPSVKIETPESFAAVDSVPVKK 130

Query: 94  PK-----------KERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEAAALAYDQ 153
            K           K + YRGVR+RPWGKFAAEIRD  ++G RVWLGTF++AE AALAYD+
Sbjct: 131 EKTSPVSAAVTAAKGKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDR 190

Query: 154 AAFSMRGAAAILNFPV-------DRVRESLKEMNCSGGGGGG 157
           AAF MRG+ A+LNFP+       D VR   K  + S     G
Sbjct: 191 AAFRMRGSRALLNFPLRVNSGEPDPVRIKSKRSSFSSSNENG 232

[Close]

BLAST of MC05g0083 vs. TAIR 10
Match: AT5G47220.1 (ethylene responsive element binding factor 2 )

HSP 1 Score: 107.1 bits (266), Expect = 1.9e-23
Identity = 78/188 (41.49%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 34  LPFNENDSEEMLLYELISEGTQ--------------GGVRVKEEEVDSVGEESPK----- 93
           LP  ENDSE+ML+Y L+ +                   V+V+  E  +  EE PK     
Sbjct: 48  LPLKENDSEDMLVYGLLKDAFHFDTSSSDLSCLFDFPAVKVEPTENFTAMEEKPKKAIPV 107

Query: 94  -----KERSYRGVRRRPWGKFAAEIRDSTRHGIRVWLGTFDSAEAAALAYDQAAFSMRGA 153
                K + YRGVR+RPWGKFAAEIRD  ++G RVWLGTF++AE AALAYD AAF MRG+
Sbjct: 108 TETAVKAKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDIAAFRMRGS 167

Query: 154 AAILNFPV-------DRVRESLKEMNCSGGGGGGGDDGGSPVVALKRKHSIRRKAISKKS 191
            A+LNFP+       D VR + K  + S           S    LKR    RRKA +  S
Sbjct: 168 RALLNFPLRVNSGEPDPVRITSKR-SSSSSSSSSSSTSSSENGKLKR----RRKAENLTS 227

The following BLAST results are available for this feature:

BLAST of MC05g0083 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LDC8	4.7e-56	58.41	Ethylene-responsive transcription factor 1B OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
A0A3Q7I5Y9	4.6e-51	58.85	Ethylene-response factor C3 OS=Solanum lycopersicum OX=4081 GN=ERF.C.3 PE=2 SV=1	[more]
Q8VYM0	8.9e-39	47.56	Ethylene-responsive transcription factor 15 OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
Q9LND1	3.2e-36	44.27	Ethylene-responsive transcription factor ERF094 OS=Arabidopsis thaliana OX=3702 ...	[more]
O04681	7.8e-27	54.31	Pathogenesis-related genes transcriptional activator PTI5 OS=Solanum lycopersicu...	[more]

BLAST of MC05g0083 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150897.1	8.25e-146	100.00	ethylene-responsive transcription factor 1B [Momordica charantia]	[more]
XP_022975053.1	1.97e-125	87.89	ethylene-responsive transcription factor 1B-like [Cucurbita maxima]	[more]
XP_038879960.1	8.60e-125	87.67	ethylene-response factor C3-like [Benincasa hispida]	[more]
KAG6597369.1	6.58e-124	87.05	Ethylene-responsive transcription factor 1B, partial [Cucurbita argyrosperma sub...	[more]
XP_022942451.1	1.55e-122	86.16	ethylene-responsive transcription factor 1B-like [Cucurbita moschata]	[more]

BLAST of MC05g0083 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DC08	3.99e-146	100.00	ethylene-responsive transcription factor 1B OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1II54	9.54e-126	87.89	ethylene-responsive transcription factor 1B-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1FNW6	7.48e-123	86.16	ethylene-responsive transcription factor 1B-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A5A7U4J3	5.59e-121	86.40	Ethylene-responsive transcription factor 1B OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S4DSE7	5.59e-121	86.40	ethylene-responsive transcription factor 1B OS=Cucumis melo OX=3656 GN=LOC103483...	[more]

BLAST of MC05g0083 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23240.1	3.3e-57	58.41	ethylene response factor 1	[more]
AT2G31230.1	6.3e-40	47.56	ethylene-responsive element binding factor 15	[more]
AT1G06160.1	2.3e-37	44.27	octadecanoid-responsive Arabidopsis AP2/ERF 59	[more]
AT4G17500.1	3.7e-24	43.21	ethylene responsive element binding factor 1	[more]
AT5G47220.1	1.9e-23	41.49	ethylene responsive element binding factor 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 79..90 score: 72.73 coord: 102..118 score: 65.03
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 78..142 e-value: 7.9E-35 score: 131.7
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 78..128 e-value: 1.3E-14 score: 54.2
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 78..136 score: 23.077003
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 79..138 e-value: 2.80227E-19 score: 76.1563
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 78..138 e-value: 9.2E-26 score: 91.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..78
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..80
None	No IPR available	PANTHER	PTHR31190:SF132	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 1B	coord: 27..209
IPR044808	Ethylene-responsive transcription factor	PANTHER	PTHR31190	DNA-BINDING DOMAIN	coord: 27..209
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 79..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0083.1	MC05g0083.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009873	ethylene-activated signaling pathway
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity