MC05g0072 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0072
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionenoyl-CoA delta isomerase 2, peroxisomal-like
LocationMC05: 509020 .. 512452 (+)
RNA-Seq ExpressionMC05g0072
SyntenyMC05g0072
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCACAGTTGAGAGGCGAGGCAATGTGTTCATCCTAACACTGACCGCCAACGACGATCACCGCCTCAACCCACCGTTGATCTACTCCATCCTCACCGCTCTTTCAGAGGTCAAAGCCAACGCCACCACCGGCTCCGTTCTCATCACAACCAACAATGGCGACAACTTCTCAAACGGCTTCGACCTCCCCTGGGCACTCGCCGCCGGCACCCTTGACGGCGCCCGTGACCGCCTCAACCACATAATTGACATCTTCAGGCCCGTCGTGGCCCAACTCCTTTCCCTTCGCATGCCTACCATTGCTGCCATTTCCGGCGACGCCGCTGCCGCCGGTCTCGCGTTGGCGGTGAGCCACGACTACTTGATCATGAGGAGCGATCTGGGCTCGCTTTTCATGCGAGAGATTGATCTGGGCCTCACTCTGCCGGACTACTTCGCGGCTCTGTTTAAGTCCAAGATGGGCTCCACTTCGGTTCAGCGCGACTTGATCCTGAGGGGCATGAAACTGAGCGGTGAAGCGGCGGTGAGAAGGGGCATTGTGGAGTCGGCGCACGACGGCGCTGGCGGCCTCATGACGGCTGCGCTCCAGTTTGGGGAGCAGCTGGCGGCGAAAAAATGGGATGGCGAAGCATATGCTGAAATCAGGAAGGGTTTGTACCCTGAGATCACTGGGGTGCTTGGATTACCCACCAAGGTCATGACCATTTCCAAACTCCCTCAACTTTGAGGTTAGGGTGAAATCTTGTCATTTCTCATTAAATATTTGATAATTAAGAAAAAATAATACAAAAAATAATGAATGATCAAATATTTAATGATGCAAAAATAAAATTAGTCCACTTAGCCAAGTATTTTACATGTCTACCTAGTTTCAAGATAAATGTCAATGGTTGACCGTACTGTGCTGATTTGATAAGAGTTATTAAATTTAATAATGTTTTTTTTTTATTGGACTTCTCTCATTTCTTCCGGTGAAACTCGCCGCCGGCCGCCGTATCCTCCTCGCCAGCCATCACTACCGCCGCCGCAAACCTCTTTCTCCCTTCCTCCGGCCGGTGAACGAGGAACGAAGCTCTGGAATGAGGATAGAATCCTCCTTTTCTGGCTCACATGGCAACTTTCCATTACAGACCATCTGTCTTCTTCTGACTTTGTATTACCGTCACGTTTCTTTGTTGTTTTTGTCGTTTCAGAAGTCTCCCATAAACATGTTTCAAAATCGCATTATCTGATATTGTTTAGAATATTTCAATTCCTCAGAAAATATTCTAAATAAAATTCATGCTATCTCGAGTCACTAAATTTTTAGTATCTGTTTTGGTACTGTGCATCATTTTGAACTTGGGCAGATCTTTTAAACGCACGCACGGGCAACAAGAAACATGACAAGTCTGGTAAGTTCACAAGGATGAACAGCTCTCTCCAGTCCCCATTCCCTAAATTTCATCCTAAATATATAGAATTAAATACTTAATCGTAAAGATCACATCTAAAGACGGAAAAGCTCAAAAACACATGGAATTGATTTACGCACCATATTTGCAGGCCAGGAAAAAAAAAAGATTACAAATCAAAAGAATTTATCATCTGCAATAAGAAACATGACAAAGTCTCAGAAATTTTCTGTATAATGACAACTGTGCACATTAAGGTTGTTACATTTGCTACAGTTATATCAAGTTCTAGAGCATTGTAAGCTCAACTTCCATTACACTAGTGGCAACAAATTTGAGCTGTTCCTGATCAGTAGGAAAATAGCAGAAACAGGTCACCTTCTAGGAATTAATGTAGCACATCGCTTGTGCTTGAAGATAATAAGAATCCTTGTATTTTCTGCCACTTCCAACAAAAATTCCACTGTTAATCCAGTCTTCACGATGTATCCAAGCATCTCGCCCGAAATCAAGCACTCCGATAATCTGTCACATTGTTGTCATATGCTATTGATTATCCTGATGGATTTTCCACACTATATTGAAAGATATTAAGAAGGTATTTTGTTCATGTATCCTTTTCCACTTGCAAATTTCCCATTCCAAAGAACAAAAACTTTTGAGTATTTATTCACTTCAAATACATTAAGAATGATTCTTCGTAGACTCTGCTGCAGCTAAGAGTTCTGACAATTTAACCTACTCGGTGTATTCTTCCTTTATTCGACATACCATGTATAGAGAGGAAATTTTCCCTAAGCTTTTCAGCAATCAGATTGTTTAAATTTCAGCAAGTTAATTCTTATAAATCCTTCACGGGAAATTGGGTAATGCCTATTCTATAAGGATAAATGACCTCGATTACATTCTGATATCAGGAAAATACAAAATAATAATAATGTGTGAAATAAAAATCTTTTAATGCCGTTCAACTTACCGCTCCACCTTTCCATGATACGAAAGAAGGGTTTATTCTTGATTGCAACACCTATTTGGAACACATCGTTCAGTCATATAATAACATTAAAGTAGAAACGAATTTATAGGTGGTAATTGATAACCTACTATTAGCATGGTACTGAATTCAATATAAACAGGCAGGAACCGCAAACCATCAAATTCCTAGATCCCTAGAATCTTTGCTAATAGTCTTTTAACTATGAAACAATTGGAAGTAATCGGCGTCAATGTCTGTACATGCAAGGGGTAGTAAAGTTATAATTAACCAAGGCACGCTTCCTATTTAGAGCAGAACTTGACTAATTACTGCCTATAGTTCAAATTTATTATAATTGTACATAATTATAATCTATATTTTGTTGTAGTTGTTCTCTCCTGTATTCTAGATGGGTAATTTATTTTATTCGATCTAGTCATTTATCCCTTTCTGTCCCGGGGTAGTTATAATTGTTATTGATGGGTGGATTCTATTTTCTTCTGTGTAATTTGTTTATCCTTAAAGGGGACAAAGCATCTCTCTCATAGATTTACAGTTATCCTTTTTCTAAGTATAATCTTTAAACAAAAAGCATGGAATTAAACTAAAAATAAAAGACCGGGGGCAATAAAAAGACCCAAAAAAGTTACATGGAGTGCTGGTAGTTGGCGACGAAGAATAGGGAAACAATACCGAATAAGAAGACGATGCCTAATGGAAGATATCTGAGTAAGATACACTCCTGTCCTCCTAATACCCCTCATTTTGAAACACCCATTTGTTTCCCTCCAGCCAAATGATCTAATATATAAGAAATAAACCAACTTGTAAAAGCAATGACCACGGCCAATAATCCTGACTTAGGGGCCAACGACACTCCAAATATTGATTTAGATCCTGCGCCACTCTATGTATAGAACACACCGCGTACTCAGAGGCCTCTATTATATAGAACACACCGCCTACTCAGAGGCCGTAAAAGACCAAACTGAACTTAAATGAAAAATGAAATCAATACATTCTATTAGTATTAGTTAAGTTTCTCAACTAATAGAGTAGAA

mRNA sequence

ATGTGCACAGTTGAGAGGCGAGGCAATGTGTTCATCCTAACACTGACCGCCAACGACGATCACCGCCTCAACCCACCGTTGATCTACTCCATCCTCACCGCTCTTTCAGAGGTCAAAGCCAACGCCACCACCGGCTCCGTTCTCATCACAACCAACAATGGCGACAACTTCTCAAACGGCTTCGACCTCCCCTGGGCACTCGCCGCCGGCACCCTTGACGGCGCCCGTGACCGCCTCAACCACATAATTGACATCTTCAGGCCCGTCGTGGCCCAACTCCTTTCCCTTCGCATGCCTACCATTGCTGCCATTTCCGGCGACGCCGCTGCCGCCGGTCTCGCGTTGGCGGTGAGCCACGACTACTTGATCATGAGGAGCGATCTGGGCTCGCTTTTCATGCGAGAGATTGATCTGGGCCTCACTCTGCCGGACTACTTCGCGGCTCTGTTTAAGTCCAAGATGGGCTCCACTTCGGTTCAGCGCGACTTGATCCTGAGGGGCATGAAACTGAGCGGTGAAGCGGCGGTGAGAAGGGGCATTGTGGAGTCGGCGCACGACGGCGCTGGCGGCCTCATGACGGCTGCGCTCCAGTTTGGGGAGCAGCTGGCGGCGAAAAAATGGGATGGCGAAGCATATGCTGAAATCAGGAAGGGTTTGTACCCTGAGATCACTGGGGTGCTTGGATTACCCACCAAGGTCATGACCATTTCCAAACTCCCTCAACTTTGAGGTTAGGGTGAAATCTTGTCATTTCTCATTAAATATTTGATAATTAAGAAAAAATAATACAAAAAATAATGAATGATCAAATATTTAATGATGCAAAAATAAAATTAGTCCACTTAGCCAAGTATTTTACATGTCTACCTAGTTTCAAGATAAATGTCAATGGTTGACCGTACTGTGCTGATTTGATAAGAGTTATTAAATTTAATAATGTTTTTTTTTTATTGGACTTCTCTCATTTCTTCCGGTGAAACTCGCCGCCGGCCGCCGTATCCTCCTCGCCAGCCATCACTACCGCCGCCGCAAACCTCTTTCTCCCTTCCTCCGGCCGGTGAACGAGGAACGAAGCTCTGGAATGAGGATAGAATCCTCCTTTTCTGGCTCACATGGCAACTTTCCATTACAGACCATCTGTCTTCTTCTGACTTTGTATTACCGTCACGTTTCTTTGTTGTTTTTGTCGTTTCAGAAGTCTCCCATAAACATGTTTCAAAATCGCATTATCTGATATTGTTTAGAATATTTCAATTCCTCAGAAAATATTCTAAATAAAATTCATGCTATCTCGAGTCACTAAATTTTTAGTATCTGTTTTGGTACTGTGCATCATTTTGAACTTGGGCAGATCTTTTAAACGCACGCACGGGCAACAAGAAACATGACAAGTCTGGTAAGTTCACAAGGATGAACAGCTCTCTCCAGTCCCCATTCCCTAAATTTCATCCTAAATATATAGAATTAAATACTTAATCGTAAAGATCACATCTAAAGACGGAAAAGCTCAAAAACACATGGAATTGATTTACGCACCATATTTGCAGGCCAGGAAAAAAAAAAGATTACAAATCAAAAGAATTTATCATCTGCAATAAGAAACATGACAAAGTCTCAGAAATTTTCTGTATAATGACAACTGTGCACATTAAGGTTGTTACATTTGCTACAGTTATATCAAGTTCTAGAGCATTGTAAGCTCAACTTCCATTACACTAGTGGCAACAAATTTGAGCTGTTCCTGATCAGTAGGAAAATAGCAGAAACAGGTCACCTTCTAGGAATTAATGTAGCACATCGCTTGTGCTTGAAGATAATAAGAATCCTTGTATTTTCTGCCACTTCCAACAAAAATTCCACTGTTAATCCAGTCTTCACGATGTATCCAAGCATCTCGCCCGAAATCAAGCACTCCGATAATCTGTCACATTGTTGTCATATGCTATTGATTATCCTGATGGATTTTCCACACTATATTGAAAGATATTAAGAAGGTATTTTGTTCATGTATCCTTTTCCACTTGCAAATTTCCCATTCCAAAGAACAAAAACTTTTGAGTATTTATTCACTTCAAATACATTAAGAATGATTCTTCGTAGACTCTGCTGCAGCTAAGAGTTCTGACAATTTAACCTACTCGGTGTATTCTTCCTTTATTCGACATACCATGTATAGAGAGGAAATTTTCCCTAAGCTTTTCAGCAATCAGATTGTTTAAATTTCAGCAAGTTAATTCTTATAAATCCTTCACGGGAAATTGGGTAATGCCTATTCTATAAGGATAAATGACCTCGATTACATTCTGATATCAGGAAAATACAAAATAATAATAATGTGTGAAATAAAAATCTTTTAATGCCGTTCAACTTACCGCTCCACCTTTCCATGATACGAAAGAAGGGTTTATTCTTGATTGCAACACCTATTTGGAACACATCGTTCAGTCATATAATAACATTAAAGTAGAAACGAATTTATAGGTGGTAATTGATAACCTACTATTAGCATGGTACTGAATTCAATATAAACAGGCAGGAACCGCAAACCATCAAATTCCTAGATCCCTAGAATCTTTGCTAATAGTCTTTTAACTATGAAACAATTGGAAGTAATCGGCGTCAATGTCTGTACATGCAAGGGGTAGTAAAGTTATAATTAACCAAGGCACGCTTCCTATTTAGAGCAGAACTTGACTAATTACTGCCTATAGTTCAAATTTATTATAATTGTACATAATTATAATCTATATTTTGTTGTAGTTGTTCTCTCCTGTATTCTAGATGGGTAATTTATTTTATTCGATCTAGTCATTTATCCCTTTCTGTCCCGGGGTAGTTATAATTGTTATTGATGGGTGGATTCTATTTTCTTCTGTGTAATTTGTTTATCCTTAAAGGGGACAAAGCATCTCTCTCATAGATTTACAGTTATCCTTTTTCTAAGTATAATCTTTAAACAAAAAGCATGGAATTAAACTAAAAATAAAAGACCGGGGGCAATAAAAAGACCCAAAAAAGTTACATGGAGTGCTGGTAGTTGGCGACGAAGAATAGGGAAACAATACCGAATAAGAAGACGATGCCTAATGGAAGATATCTGAGTAAGATACACTCCTGTCCTCCTAATACCCCTCATTTTGAAACACCCATTTGTTTCCCTCCAGCCAAATGATCTAATATATAAGAAATAAACCAACTTGTAAAAGCAATGACCACGGCCAATAATCCTGACTTAGGGGCCAACGACACTCCAAATATTGATTTAGATCCTGCGCCACTCTATGTATAGAACACACCGCGTACTCAGAGGCCTCTATTATATAGAACACACCGCCTACTCAGAGGCCGTAAAAGACCAAACTGAACTTAAATGAAAAATGAAATCAATACATTCTATTAGTATTAGTTAAGTTTCTCAACTAATAGAGTAGAA

Coding sequence (CDS)

ATGTGCACAGTTGAGAGGCGAGGCAATGTGTTCATCCTAACACTGACCGCCAACGACGATCACCGCCTCAACCCACCGTTGATCTACTCCATCCTCACCGCTCTTTCAGAGGTCAAAGCCAACGCCACCACCGGCTCCGTTCTCATCACAACCAACAATGGCGACAACTTCTCAAACGGCTTCGACCTCCCCTGGGCACTCGCCGCCGGCACCCTTGACGGCGCCCGTGACCGCCTCAACCACATAATTGACATCTTCAGGCCCGTCGTGGCCCAACTCCTTTCCCTTCGCATGCCTACCATTGCTGCCATTTCCGGCGACGCCGCTGCCGCCGGTCTCGCGTTGGCGGTGAGCCACGACTACTTGATCATGAGGAGCGATCTGGGCTCGCTTTTCATGCGAGAGATTGATCTGGGCCTCACTCTGCCGGACTACTTCGCGGCTCTGTTTAAGTCCAAGATGGGCTCCACTTCGGTTCAGCGCGACTTGATCCTGAGGGGCATGAAACTGAGCGGTGAAGCGGCGGTGAGAAGGGGCATTGTGGAGTCGGCGCACGACGGCGCTGGCGGCCTCATGACGGCTGCGCTCCAGTTTGGGGAGCAGCTGGCGGCGAAAAAATGGGATGGCGAAGCATATGCTGAAATCAGGAAGGGTTTGTACCCTGAGATCACTGGGGTGCTTGGATTACCCACCAAGGTCATGACCATTTCCAAACTCCCTCAACTTTGA

Protein sequence

MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNGFDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHDYLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGIVESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKLPQL
Homology
BLAST of MC05g0072 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 2.6e-71
Identity = 132/240 (55.00%), Postives = 184/240 (76.67%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCT+E+RG++F+LTLT + +HR +P  I +IL+ L + K+ +T GS+LITT NG  FSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDL WA  AG+  GA +RL+ +++ F+PVVA LL L MPTIAA++G AAAAGL LA+SHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           Y+ MR D G L+M E+D+GL++PDYF+AL ++K+G+++ +R+L+L G K+ GE AV  GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           V+S A+D   G++ A+++ GE+LAAKKW GE YA IRK LYPE+ G+LGL T+V    KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

BLAST of MC05g0072 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.1e-64
Identity = 126/230 (54.78%), Postives = 169/230 (73.48%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCT+E+RG++F+LTLT  D+HR +P  I S+L+ L + K+ +T GSVLITT +G  FSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDL WA +AG   GA  R++ ++  F+PV+A LL L MPTIAA++G AAA+GL  A+SHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           Y+ MR D G L+M E+D+GL +PDYF+AL  +K+GS   +R+L+L G KL GE AV  GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGL 230
           V+S AHD A G++ A +  GE LAAKKW+GE YA IRK LYPE+  ++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

BLAST of MC05g0072 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.5e-42
Identity = 91/224 (40.62%), Postives = 150/224 (66.96%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANAT-TGSVLITTNNGDNFSN 60
           MC++E+R  +FIL LT + +HRLNP L+ S+ + +++++++ + + SVLITT++G  FSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSH 120
           G+DL  A +  +L    D         R +VA L+SL MPTIAA++G A+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRG 180
           DY++MR D G L+M E+D+ L +P +F A+ + K+GS + +RD++L   K++ +  V+ G
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEI 224
           IV+SA+  A   + AA++ GE++  +  DG  Y ++R+ L  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

BLAST of MC05g0072 vs. ExPASy Swiss-Prot
Match: A3QFP3 (Fatty acid oxidation complex subunit alpha OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) OX=323850 GN=fadJ PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.9e-06
Identity = 45/153 (29.41%), Postives = 76/153 (49.67%), Query Frame = 0

Query: 31  ILTALSEVKANATTGSVLITTNNGDNFSNGFDLPWALAAGTLDGARDRLNHIIDIFRPVV 90
           I   L+E+KA+A    V+I +   D+F  G D+    A  T + AR+      ++F    
Sbjct: 37  ICEMLAEIKADAGIRGVVIISGKKDSFVAGADISMLDACATAEDARELSKQGHEVF---- 96

Query: 91  AQLLSLRMPTIAAISGDAAAAGLALAVS-HDYLIMRSDLGSLFMREIDLGLTLPDYFAAL 150
             L SL +P +AAI+G     GL LA++ H  +   S+   L + E+ LGL LP      
Sbjct: 97  FALESLSIPVVAAINGACLGGGLELALACHQRVCTDSNKTMLGLPEVQLGL-LPGGGGTQ 156

Query: 151 FKSKMGSTSVQRDLILRGMKLSGEAAVRRGIVE 183
              ++   +   D++L G +L  + A++ G+V+
Sbjct: 157 RLPRLVGIAKSLDMMLTGKQLRAKQALKMGLVD 184

BLAST of MC05g0072 vs. ExPASy Swiss-Prot
Match: P64019 (Probable enoyl-CoA hydratase echA14 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=echA14 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 5.0e-06
Identity = 64/197 (32.49%), Postives = 88/197 (44.67%), Query Frame = 0

Query: 12  ILTLTANDDHRLN---PPLIYSILTALSEVKANATTGSVLITTNNGDNFSNGFDLPWALA 71
           +  +T ND  R N     +   +  A+   + +    +V++ T  G  F  G DL  AL 
Sbjct: 15  VALITVNDPDRRNAVTDEMSAQLRAAIQRAEGDPDVHAVVV-TGAGKAFCAGADLS-ALG 74

Query: 72  AGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHDYLIMRSDL 131
           AG  D A  RL  + D F  V     S  +PTIAA++G A  AGL LA++ D  I  +  
Sbjct: 75  AGVGDPAEPRLLRLYDGFMAV----SSCNLPTIAAVNGAAVGAGLNLALAADVRI--AGP 134

Query: 132 GSLF-MREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGIVESAHDG 191
            +LF  R   LGL  P   A     +     V R  +L GM    E+AVR G+     D 
Sbjct: 135 AALFDARFQKLGLH-PGGGATWMLQRAVGPQVARAALLFGMCFDAESAVRHGLALMVADD 194

Query: 192 AGGLMTAALQFGEQLAA 205
               +TAAL+     AA
Sbjct: 195 P---VTAALELAAGPAA 199

BLAST of MC05g0072 vs. NCBI nr
Match: XP_022143599.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Momordica charantia] >XP_022143606.1 enoyl-CoA delta isomerase 2, peroxisomal-like [Momordica charantia])

HSP 1 Score: 465 bits (1196), Expect = 7.89e-165
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG
Sbjct: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD
Sbjct: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI
Sbjct: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKLP 240
           VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKLP
Sbjct: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKLP 240

Query: 241 QL 242
           QL
Sbjct: 241 QL 242

BLAST of MC05g0072 vs. NCBI nr
Match: KAG6582371.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 345 bits (885), Expect = 1.70e-117
Identity = 179/239 (74.90%), Postives = 204/239 (85.36%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCTVE+RGN FILTLT +DDHRL P LI SIL ALS+VKA AT+GSVLITT++G  FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRLNHII IF+P+VAQLLSL MPTIAAI+G AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 239
           VES H G  G+  AA+Q GEQLAA+KW+GE YAEIRK LYPE++ VLGL  K ++ISKL
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239

BLAST of MC05g0072 vs. NCBI nr
Match: XP_023526416.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 345 bits (884), Expect = 2.41e-117
Identity = 178/239 (74.48%), Postives = 203/239 (84.94%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCTVE+RGN FILTLT +DDHRL P LI S+L ALS+VKA AT GSVLITT++G  FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRL+HII IF+P+VAQLLSL MPTIAAISG AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLSHIIQIFKPLVAQLLSLPMPTIAAISGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLAMRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 239
           VES H G  G+  AA+Q GEQLAA+KWDGE YAEIRK +YPE++ VLGL  K ++ISKL
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWDGEVYAEIRKSVYPEVSEVLGLRGKAISISKL 239

BLAST of MC05g0072 vs. NCBI nr
Match: XP_022979202.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 343 bits (879), Expect = 1.39e-116
Identity = 178/239 (74.48%), Postives = 202/239 (84.52%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCT+E+RGN FILTLT +DDHRL P LI SIL ALS+VKA AT GSVLITT++G  FSNG
Sbjct: 1   MCTLEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRLNHII IF+P+VAQLLSL MPTIAAI+G AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALF SKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFMSKMGSSLAMRDLILRGMKVRGEAAVRIGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 239
           VES H G  G+  AA+Q GEQLAA+KWDGE YAEIRK LYPE++ VLGL  K ++ISKL
Sbjct: 181 VESTHHGEDGVANAAVQLGEQLAARKWDGEVYAEIRKSLYPEVSEVLGLRGKAISISKL 239

BLAST of MC05g0072 vs. NCBI nr
Match: XP_022924234.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 341 bits (875), Expect = 5.65e-116
Identity = 177/239 (74.06%), Postives = 202/239 (84.52%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCTVE+RGN FILTLT +DDHRL P LI S+L ALS+VKA AT GSVLITT++G  FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRLNHII IF+P+VAQLLSL MPTIAAI+G AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 239
           VES H G  G+  AA+Q GEQLAA+KW+GE YAEIRK LYPE++ VLGL  K + IS+L
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPIFISRL 239

BLAST of MC05g0072 vs. ExPASy TrEMBL
Match: A0A6J1CQS5 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=LOC111013455 PE=4 SV=1)

HSP 1 Score: 465 bits (1196), Expect = 3.82e-165
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG
Sbjct: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD
Sbjct: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI
Sbjct: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKLP 240
           VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKLP
Sbjct: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKLP 240

Query: 241 QL 242
           QL
Sbjct: 241 QL 242

BLAST of MC05g0072 vs. ExPASy TrEMBL
Match: A0A6J1IN54 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111479008 PE=4 SV=1)

HSP 1 Score: 343 bits (879), Expect = 6.73e-117
Identity = 178/239 (74.48%), Postives = 202/239 (84.52%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCT+E+RGN FILTLT +DDHRL P LI SIL ALS+VKA AT GSVLITT++G  FSNG
Sbjct: 1   MCTLEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRLNHII IF+P+VAQLLSL MPTIAAI+G AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALF SKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFMSKMGSSLAMRDLILRGMKVRGEAAVRIGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 239
           VES H G  G+  AA+Q GEQLAA+KWDGE YAEIRK LYPE++ VLGL  K ++ISKL
Sbjct: 181 VESTHHGEDGVANAAVQLGEQLAARKWDGEVYAEIRKSLYPEVSEVLGLRGKAISISKL 239

BLAST of MC05g0072 vs. ExPASy TrEMBL
Match: A0A6J1E8J9 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431761 PE=4 SV=1)

HSP 1 Score: 341 bits (875), Expect = 2.74e-116
Identity = 177/239 (74.06%), Postives = 202/239 (84.52%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCTVE+RGN FILTLT +DDHRL P LI S+L ALS+VKA AT GSVLITT++G  FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRLNHII IF+P+VAQLLSL MPTIAAI+G AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 239
           VES H G  G+  AA+Q GEQLAA+KW+GE YAEIRK LYPE++ VLGL  K + IS+L
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPIFISRL 239

BLAST of MC05g0072 vs. ExPASy TrEMBL
Match: A0A0A0L9X4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 329 bits (844), Expect = 1.43e-111
Identity = 169/239 (70.71%), Postives = 201/239 (84.10%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCTVE+RGN FILTLT +DDHRL+P LI SIL ALS+VKA A+  SVLITT++G  FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWAL+AG++  AR RLNH+I+IF+ VVA+L+SL MPTIAAI+G AAAAG  LA+SHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSK+GS+   RDLILRGMK+ GEAAVR GI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 239
           V+SAHDG   ++ AA++ GEQLA + WDGE YAEIRK LYPE++ VLGLP K ++ISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

BLAST of MC05g0072 vs. ExPASy TrEMBL
Match: A0A1S3AWB3 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483618 PE=4 SV=1)

HSP 1 Score: 325 bits (833), Expect = 6.73e-110
Identity = 169/239 (70.71%), Postives = 198/239 (82.85%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCTVE+RGN FILTLT +DDHRL+P LI SIL ALS+VKA A+  SVLITT++G  FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++  AR RLNHII+IF+ VVA+L+SL MPTIAAI+G AAAAG  LA+SHD
Sbjct: 61  FDLPWALAAGSVSAARHRLNHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSK+GS+   RDLILRGMK+ GEAAVR GI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 239
           V+SAH     ++ AA++ GEQL  + WDGE YAEIRK LYPEI+ VLGLP K ++ISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

BLAST of MC05g0072 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 270.0 bits (689), Expect = 1.8e-72
Identity = 132/240 (55.00%), Postives = 184/240 (76.67%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCT+E+RG++F+LTLT + +HR +P  I +IL+ L + K+ +T GS+LITT NG  FSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDL WA  AG+  GA +RL+ +++ F+PVVA LL L MPTIAA++G AAAAGL LA+SHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           Y+ MR D G L+M E+D+GL++PDYF+AL ++K+G+++ +R+L+L G K+ GE AV  GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           V+S A+D   G++ A+++ GE+LAAKKW GE YA IRK LYPE+ G+LGL T+V    KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

BLAST of MC05g0072 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 246.5 bits (628), Expect = 2.2e-65
Identity = 126/230 (54.78%), Postives = 169/230 (73.48%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60
           MCT+E+RG++F+LTLT  D+HR +P  I S+L+ L + K+ +T GSVLITT +G  FSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDL WA +AG   GA  R++ ++  F+PV+A LL L MPTIAA++G AAA+GL  A+SHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           Y+ MR D G L+M E+D+GL +PDYF+AL  +K+GS   +R+L+L G KL GE AV  GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGL 230
           V+S AHD A G++ A +  GE LAAKKW+GE YA IRK LYPE+  ++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

BLAST of MC05g0072 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 173.7 bits (439), Expect = 1.8e-43
Identity = 91/224 (40.62%), Postives = 150/224 (66.96%), Query Frame = 0

Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANAT-TGSVLITTNNGDNFSN 60
           MC++E+R  +FIL LT + +HRLNP L+ S+ + +++++++ + + SVLITT++G  FSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSH 120
           G+DL  A +  +L    D         R +VA L+SL MPTIAA++G A+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRG 180
           DY++MR D G L+M E+D+ L +P +F A+ + K+GS + +RD++L   K++ +  V+ G
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEI 224
           IV+SA+  A   + AA++ GE++  +  DG  Y ++R+ L  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O232992.6e-7155.00Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2... [more]
O233003.1e-6454.78Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1[more]
O044692.5e-4240.63Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1... [more]
A3QFP32.9e-0629.41Fatty acid oxidation complex subunit alpha OS=Shewanella loihica (strain ATCC BA... [more]
P640195.0e-0632.49Probable enoyl-CoA hydratase echA14 OS=Mycobacterium bovis (strain ATCC BAA-935 ... [more]
Match NameE-valueIdentityDescription
XP_022143599.17.89e-165100.00enoyl-CoA delta isomerase 2, peroxisomal-like [Momordica charantia] >XP_02214360... [more]
KAG6582371.11.70e-11774.90Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023526416.12.41e-11774.48enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo][more]
XP_022979202.11.39e-11674.48enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima][more]
XP_022924234.15.65e-11674.06enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1CQS53.82e-165100.00enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1IN546.73e-11774.48enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1E8J92.74e-11674.06enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A0A0L9X41.43e-11170.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1[more]
A0A1S3AWB36.73e-11070.71enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT4G14430.11.8e-7255.00indole-3-butyric acid response 10 [more]
AT4G14440.12.2e-6554.783-hydroxyacyl-CoA dehydratase 1 [more]
AT1G65520.11.8e-4340.63delta(3), delta(2)-enoyl CoA isomerase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.226.10coord: 1..226
e-value: 8.1E-34
score: 118.8
NoneNo IPR availablePANTHERPTHR11941:SF75ENOYL-COA DELTA ISOMERASE 2, PEROXISOMALcoord: 1..233
NoneNo IPR availablePANTHERPTHR11941ENOYL-COA HYDRATASE-RELATEDcoord: 1..233
NoneNo IPR availableCDDcd06558crotonase-likecoord: 2..202
e-value: 1.30347E-16
score: 73.3636
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 21..206
e-value: 8.0E-15
score: 54.8
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 3..207

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0072.1MC05g0072.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006635 fatty acid beta-oxidation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004165 dodecenoyl-CoA delta-isomerase activity
molecular_function GO:0004300 enoyl-CoA hydratase activity
molecular_function GO:0003824 catalytic activity