MC05g0062 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g0062 vs. ExPASy Swiss-Prot
Match: O23304 (Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 PE=1 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.2e-144
Identity = 278/497 (55.94%), Postives = 350/497 (70.42%), Query Frame = 0

Query: 19  QFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIRREAQIL 78
           +FPLD+ +Y II +IGVGVSA VYKAICIPMNS VVAIK+IDLD+SRADFD +RRE + +
Sbjct: 7   EFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLDQSRADFDSLRRETKTM 66

Query: 79  SLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAVVLREILSA 138
           SLLSHPNILNA+CSFTVDR LWVVMPFMS GSL SI++SSFP GLPE CI+V L+E L+A
Sbjct: 67  SLLSHPNILNAYCSFTVDRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKETLNA 126

Query: 139 LAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMMKLT 198
           ++YLHDQGHLHRDIKAGNIL+DS+GSVKLADFGVS SI+E       S+S  ++S ++LT
Sbjct: 127 ISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVT----SSSGTTSSSLRLT 186

Query: 199 DVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKITRRF 258
           D+AGTPYWMAPEV+HSH GY  KADIWSFGITALELA GRPPLSHLPP KSLL+KIT+RF
Sbjct: 187 DIAGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKRF 246

Query: 259 RFSDYD-----NGRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVL 318
            FSDY+     + +    KFSK F+E+V  CL+Q+P+KRP A+KLLKH FF+NCKG D +
Sbjct: 247 HFSDYEINTSGSSKKGNKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKGLDFV 306

Query: 319 VRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELE-EEIIKQRRISGWNFNEDGLE 378
           V+ VL  L + E+ F   ++     +K  GD+ EE E E EEI+K RRISGWNF ED L+
Sbjct: 307 VKNVLHSLSNAEQMFMESQI----LIKSVGDDDEEEEEEDEEIVKNRRISGWNFREDDLQ 366

Query: 379 LDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGLSSPSQTGNDGDRRSGG 438
           L PVFP    +  E            S  +E  S D  E  +   +  + G         
Sbjct: 367 LSPVFPATESDSSE-----------SSPREEDQSKDKKEDDNVTITGYELGLGLSNEEAK 426

Query: 439 GGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEKNGEMNREEQMVRLIEKM 498
              GE    DK+ ++  L  LK+SL+ QR  V  +IE L G+K  E +REE+++ ++EK+
Sbjct: 427 NQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKE-EKSREEELLEMVEKL 483

Query: 499 TQELENERNRNFELEME 510
             ELE E+ +    + +
Sbjct: 487 KIELETEKLKTLRADKD 483

BLAST of MC05g0062 vs. ExPASy Swiss-Prot
Match: Q551H4 (Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fray2 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.9e-66
Identity = 136/315 (43.17%), Postives = 190/315 (60.32%), Query Frame = 0

Query: 17  RPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSR-ADFDDIRREA 76
           R  +P  +  Y + + IG G S +V +AIC+P     VAIK IDL+  +    ++IR+E 
Sbjct: 61  RKNYPNSADQYELKETIGKGGSGLVQRAICLPFQEN-VAIKIIDLEHCKNVSLEEIRKEI 120

Query: 77  QILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAVVLREI 136
           Q +SL  HPN++  H SF  +  LWV+M F+SAGS   I+  SFP G  E  IA +L+E 
Sbjct: 121 QAMSLCHHPNVVAYHTSFVYNESLWVIMDFLSAGSCSDIMRFSFPQGFEEHVIATILKEA 180

Query: 137 LSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMM 196
           L A+ Y H  G +HRDIK+GNIL+DSNG+++L+DFGVS ++        + T   S +  
Sbjct: 181 LKAICYFHKTGRIHRDIKSGNILIDSNGNIQLSDFGVSATL--------IDTGETSRNTF 240

Query: 197 KLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKIT 256
                 GTP WMAPE++   N Y    DIWSFGITALELA G+ P +  PP K LLL + 
Sbjct: 241 -----VGTPCWMAPEIMEQVN-YDYAVDIWSFGITALELARGKAPFAEYPPMKVLLLTLQ 300

Query: 257 RRFRFSDYDNGRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVLVR 316
                 + D     ++K+S  FK+LV  CL ++PSKRP   KLL+H FF+  K PD LV+
Sbjct: 301 NPPPSLEGD----GESKWSHSFKDLVEKCLQKDPSKRPLPSKLLEHRFFKQAKKPDYLVQ 356

Query: 317 TVLQGLPSVEERFKL 331
            +L  LP + +R+++
Sbjct: 361 HILAKLPPLGQRYQM 356

BLAST of MC05g0062 vs. ExPASy Swiss-Prot
Match: Q6P9R2 (Serine/threonine-protein kinase OSR1 OS=Mus musculus OX=10090 GN=Oxsr1 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 3.4e-60
Identity = 138/366 (37.70%), Postives = 202/366 (55.19%), Query Frame = 0

Query: 13  EEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIR 72
           E+     + ++   Y + + IG G +AVV  A C P     VAIK I+L++ +   D++ 
Sbjct: 3   EDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKER-VAIKRINLEKCQTSMDELL 62

Query: 73  REAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDG------LPEP 132
           +E Q +S   HPNI++ + SF V   LW+VM  +S GS+  II      G      L EP
Sbjct: 63  KEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDEP 122

Query: 133 CIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLS 192
            IA +LRE+L  L YLH  G +HRD+KAGNIL+  +GSV++ADFGVS  +         +
Sbjct: 123 TIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFL--------AT 182

Query: 193 TSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPP 252
              ++ + ++ T V GTP WMAPEV+    GY  KADIWSFGITA+ELA G  P    PP
Sbjct: 183 GGDITRNKVRKTFV-GTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPP 242

Query: 253 SKSLLLKITRRFRFSDYDNGRNSK---NKFSKGFKELVASCLDQEPSKRPCAQKLLKHSF 312
            K L+L  T +      + G   K    K+ K F+++++ CL ++P KRP A +LL+H F
Sbjct: 243 MKVLML--TLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKF 302

Query: 313 FRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEIIKQRRISG 370
           F+  K  + L   +LQ  P++ ER K  K++ +P     G  G   + E+         G
Sbjct: 303 FQKAKNKEFLQEKILQRAPTISERSK--KVRRVP-----GSSGRLHKTED--------GG 341

BLAST of MC05g0062 vs. ExPASy Swiss-Prot
Match: Q863I2 (Serine/threonine-protein kinase OSR1 OS=Sus scrofa OX=9823 GN=OXSR1 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 2.2e-59
Identity = 137/366 (37.43%), Postives = 201/366 (54.92%), Query Frame = 0

Query: 13  EEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIR 72
           E+     + ++   Y + + IG G +AVV  A C P     VAIK I+L++ +   D++ 
Sbjct: 3   EDSSALPWSINKDDYELQEVIGSGATAVVQAAYCTPKKEK-VAIKRINLEKCQTSMDELL 62

Query: 73  REAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDG------LPEP 132
           +E Q +S   HPNI++ + SF V   LW+VM  +S GS+  II      G      L E 
Sbjct: 63  KEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDEA 122

Query: 133 CIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLS 192
            IA +LRE+L  L YLH  G +HRD+KAGNIL+  +GSV++ADFGVS  +         +
Sbjct: 123 TIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFL--------AT 182

Query: 193 TSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPP 252
              ++ + ++ T V GTP WMAPEV+    GY  KADIWSFGITA+ELA G  P    PP
Sbjct: 183 GGDITRNKVRKTFV-GTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPP 242

Query: 253 SKSLLLKITRRFRFSDYDNGRNSK---NKFSKGFKELVASCLDQEPSKRPCAQKLLKHSF 312
            K L+L  T +      + G   K    K+ K F+++++ CL ++P KRP A +LL+H F
Sbjct: 243 MKVLML--TLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKF 302

Query: 313 FRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEIIKQRRISG 370
           F+  K  + L   +LQ  P++ ER K  K++ +P     G  G   + E+         G
Sbjct: 303 FQKAKNKEYLQEKILQRAPTISERAK--KVRRVP-----GSSGRLHKTED--------GG 341

BLAST of MC05g0062 vs. ExPASy Swiss-Prot
Match: Q5R495 (Serine/threonine-protein kinase OSR1 OS=Pongo abelii OX=9601 GN=OXSR1 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.9e-59
Identity = 137/366 (37.43%), Postives = 201/366 (54.92%), Query Frame = 0

Query: 13  EEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIR 72
           E+     + ++   Y + + IG G +AVV  A C P     VAIK I+L++ +   D++ 
Sbjct: 3   EDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEK-VAIKRINLEKCQTSMDELL 62

Query: 73  REAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDG------LPEP 132
           +E Q +S   HPNI++ + SF V   LW+VM  +S GS+  II      G      L E 
Sbjct: 63  KEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDES 122

Query: 133 CIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLS 192
            IA +LRE+L  L YLH  G +HRD+KAGNIL+  +GSV++ADFGVS  +         +
Sbjct: 123 TIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFL--------AT 182

Query: 193 TSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPP 252
              ++ + ++ T V GTP WMAPEV+    GY  KADIWSFGITA+ELA G  P    PP
Sbjct: 183 GGDITRNKVRKTFV-GTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPP 242

Query: 253 SKSLLLKITRRFRFSDYDNGRNSK---NKFSKGFKELVASCLDQEPSKRPCAQKLLKHSF 312
            K L+L  T +      + G   K    K+ K F+++++ CL ++P KRP A +LL+H F
Sbjct: 243 MKVLML--TLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKF 302

Query: 313 FRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEIIKQRRISG 370
           F+  K  + L   +LQ  P++ ER K  K++ +P     G  G   + E+         G
Sbjct: 303 FQKAKNKEFLQEKILQRAPTISERAK--KVRRVP-----GSSGRLHKTED--------GG 341

BLAST of MC05g0062 vs. NCBI nr
Match: XP_022980836.1 (serine/threonine-protein kinase BLUS1 [Cucurbita maxima])

HSP 1 Score: 832 bits (2150), Expect = 1.30e-300
Identity = 436/526 (82.89%), Postives = 476/526 (90.49%), Query Frame = 0

Query: 2   IQSGEVIM---NDQEEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKS 61
           +QSGE+IM   +DQ+E KRPQFPLDSS Y+IIDEIGVGVSAVVYKAICIPMNSAVVAIK+
Sbjct: 12  VQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIKA 71

Query: 62  IDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 121
           IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS
Sbjct: 72  IDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 131

Query: 122 FPDGLPEPCIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFE 181
           FPDGLPEPCIAVVLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIFE
Sbjct: 132 FPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIFE 191

Query: 182 ANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGR 241
           AN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA GR
Sbjct: 192 ANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHGR 251

Query: 242 PPLSHLPPSKSLLLKITRRFRFSDYDNGRNS--KNKFSKGFKELVASCLDQEPSKRPCAQ 301
           PPLSHLPPSKSLLLKITRRFRFSDY+N +NS  KNKFSKGFKELVASCLDQEPS+RPCA 
Sbjct: 252 PPLSHLPPSKSLLLKITRRFRFSDYENQQNSNNKNKFSKGFKELVASCLDQEPSRRPCAD 311

Query: 302 KLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEII 361
           KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQ LPS+K++  E +E E+E+EII
Sbjct: 312 KLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKME--EEDEEEIEKEII 371

Query: 362 KQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGL 421
           KQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG+
Sbjct: 372 KQRRISGWNFKEDGLELEPVFPGENCEEDECSIIKQVRFGGESIM-EKGSRDSSECCSGV 431

Query: 422 SSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEKN 481
           S  S    +G R   GGG  EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK 
Sbjct: 432 SDLSSPTGNGRR---GGGEAEEEAVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEKG 491

Query: 482 GEMNREEQMVRLIEKMTQELENERNRNFELEMELEFLRLQISPAYN 522
           GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEFL LQIS A+N
Sbjct: 492 GEMSREEQIGRLMEKMTRELENERKRNFELEMELEFLGLQISAAHN 530

BLAST of MC05g0062 vs. NCBI nr
Match: XP_023526743.1 (serine/threonine-protein kinase BLUS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 828 bits (2140), Expect = 4.49e-299
Identity = 436/529 (82.42%), Postives = 475/529 (89.79%), Query Frame = 0

Query: 2   IQSGEVIM---NDQEEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKS 61
           +QSGE+IM   +DQ+E KRPQFPLDSS Y+IIDEIGVGVSAVVYKAICIPMNSAVVAIK+
Sbjct: 12  VQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIKA 71

Query: 62  IDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 121
           IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS
Sbjct: 72  IDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 131

Query: 122 FPDGLPEPCIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFE 181
           FPDGLPEPCIAVVLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIFE
Sbjct: 132 FPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIFE 191

Query: 182 ANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGR 241
           AN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA GR
Sbjct: 192 ANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHGR 251

Query: 242 PPLSHLPPSKSLLLKITRRFRFSDYDNGRNS--KNKFSKGFKELVASCLDQEPSKRPCAQ 301
           PPLSHLPPSKSLLLKITRRFRFSDY+N +NS  KNKFSKGFKELVASCLDQEPS+RPCA 
Sbjct: 252 PPLSHLPPSKSLLLKITRRFRFSDYENQQNSNNKNKFSKGFKELVASCLDQEPSRRPCAD 311

Query: 302 KLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEII 361
           KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQ LPS+K +  E +E E+E+EII
Sbjct: 312 KLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKTE--EEDEEEIEKEII 371

Query: 362 KQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSG- 421
           KQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG 
Sbjct: 372 KQRRISGWNFKEDGLELEPVFPGENCEEDECSIIKQVRFGGESIM-EKGSRDSSECCSGV 431

Query: 422 --LSSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEG 481
             LSSP+  G  G+         EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EG
Sbjct: 432 SDLSSPTGNGRRGEVEE-----EEEEGVDRESIMGGLVALKRSLDEQRRAVGSLIELIEG 491

Query: 482 EKNGEMNREEQMVRLIEKMTQELENERNRNFELEMELEFLRLQISPAYN 522
           EK GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEFLRLQIS A+N
Sbjct: 492 EKGGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFLRLQISAAHN 531

BLAST of MC05g0062 vs. NCBI nr
Match: KAG6582364.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 827 bits (2135), Expect = 2.59e-298
Identity = 435/524 (83.02%), Postives = 473/524 (90.27%), Query Frame = 0

Query: 2   IQSGEVIM---NDQEEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKS 61
           +QSGE+IM   +DQ+E KRPQFPLDSS Y+IIDEIGVGVSAVVYKAICIPMNSAVVAIK+
Sbjct: 12  VQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIKA 71

Query: 62  IDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 121
           IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS
Sbjct: 72  IDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 131

Query: 122 FPDGLPEPCIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFE 181
           FPDGLPEPCIAVVLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIFE
Sbjct: 132 FPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIFE 191

Query: 182 ANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGR 241
           AN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA GR
Sbjct: 192 ANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHGR 251

Query: 242 PPLSHLPPSKSLLLKITRRFRFSDYDNGRNS--KNKFSKGFKELVASCLDQEPSKRPCAQ 301
           PPLSHLPPSKSLLLKITRRFRFSDY+N +NS  KNKFSKGFKELVASCLDQEPS+RPCA 
Sbjct: 252 PPLSHLPPSKSLLLKITRRFRFSDYENQQNSNNKNKFSKGFKELVASCLDQEPSRRPCAD 311

Query: 302 KLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEII 361
           KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQ LPS+K + D+ EE  +E+EII
Sbjct: 312 KLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKTEEDDEEE--IEKEII 371

Query: 362 KQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGL 421
           KQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG+
Sbjct: 372 KQRRISGWNFKEDGLELEPVFPGENCEEDECSIIKQVRFGGESIM-EKGSRDSSECCSGV 431

Query: 422 SSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEKN 481
           S  S    +G R   GG   EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK 
Sbjct: 432 SDLSSPTGNGRR---GGVEEEEEGVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEKG 491

Query: 482 GEMNREEQMVRLIEKMTQELENERNRNFELEMELEFLRLQISPA 520
           GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEFLRLQIS A
Sbjct: 492 GEMSREEQIGRLMEKMTRELENERKRNFELEMELEFLRLQISAA 528

BLAST of MC05g0062 vs. NCBI nr
Match: XP_022924307.1 (serine/threonine-protein kinase BLUS1 [Cucurbita moschata])

HSP 1 Score: 826 bits (2134), Expect = 3.68e-298
Identity = 436/527 (82.73%), Postives = 476/527 (90.32%), Query Frame = 0

Query: 2   IQSGEVIM---NDQEEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKS 61
           +QSGE+IM   +DQ+E KRPQFPLDSS Y+IIDEIGVGVSAVVYKAICIPMNSAVVAIK+
Sbjct: 12  VQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIKA 71

Query: 62  IDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 121
           IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS
Sbjct: 72  IDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 131

Query: 122 FPDGLPEPCIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFE 181
           FPDGLPEPCIAVVLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIFE
Sbjct: 132 FPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIFE 191

Query: 182 ANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGR 241
           AN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA GR
Sbjct: 192 ANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHGR 251

Query: 242 PPLSHLPPSKSLLLKITRRFRFSDYDNGR--NSKNKFSKGFKELVASCLDQEPSKRPCAQ 301
           PPLSHLPPSKSLLLKITRRFRFSDY+N +  N+KNKFSKGFKELVASCLDQEPS+RPCA 
Sbjct: 252 PPLSHLPPSKSLLLKITRRFRFSDYENQQSSNNKNKFSKGFKELVASCLDQEPSRRPCAD 311

Query: 302 KLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEII 361
           KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQ LPS+K +  E +E E+E+EII
Sbjct: 312 KLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKTE--EEDEEEIEKEII 371

Query: 362 KQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGL 421
           KQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG+
Sbjct: 372 KQRRISGWNFKEDGLELEPVFPGENCEEDECSIMKQVRFGGESIM-EKGSRDSSECCSGV 431

Query: 422 SS-PSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 481
           S   S TGN    R GG    EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK
Sbjct: 432 SDLSSPTGNG---RRGGVEEEEEEGVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEK 491

Query: 482 NGEMNREEQMVRLIEKMTQELENERNRNFELEMELEFLRLQISPAYN 522
            GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEF+RLQIS A+N
Sbjct: 492 GGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFVRLQISAAHN 531

BLAST of MC05g0062 vs. NCBI nr
Match: KAG7018775.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 824 bits (2128), Expect = 3.37e-297
Identity = 435/530 (82.08%), Postives = 473/530 (89.25%), Query Frame = 0

Query: 2   IQSGEVIM---NDQEEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKS 61
           +QSGE+IM   +DQ+E KRPQFPLDSS Y+IIDEIGVGVSAVVYKAICIPMNSAVVAIK+
Sbjct: 12  VQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIKA 71

Query: 62  IDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 121
           IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS
Sbjct: 72  IDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 131

Query: 122 FPDGLPEPCIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFE 181
           FPDGLPEPCIAVVLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIFE
Sbjct: 132 FPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIFE 191

Query: 182 ANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGR 241
           AN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA GR
Sbjct: 192 ANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHGR 251

Query: 242 PPLSHLPPSKSLLLKITRRFRFSDYDNGRNS--KNKFSKGFKELVASCLDQEPSKRPCAQ 301
           PPLSHLPPSKSLLLKITRRFRFSDY+N +NS  KNKFSKGFKELVASCLDQEPS+RPCA 
Sbjct: 252 PPLSHLPPSKSLLLKITRRFRFSDYENQQNSNNKNKFSKGFKELVASCLDQEPSRRPCAD 311

Query: 302 KLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEII 361
           KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQ LPS+K + D+ EE  +E+EII
Sbjct: 312 KLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKTEEDDEEE--IEKEII 371

Query: 362 KQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGL 421
           KQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG+
Sbjct: 372 KQRRISGWNFKEDGLELEPVFPGENCEEDECSIIKQVRFGGESIM-EKGSRDSSECCSGV 431

Query: 422 SSPSQTGNDGDRRSGGGGGG------EEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEF 481
           S  S    +G R      GG      EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE 
Sbjct: 432 SDLSSPTGNGRR------GGVEEEEEEEEGVDRESIMGGLVALKRSLDEQRRAVGSLIEL 491

Query: 482 LEGEKNGEMNREEQMVRLIEKMTQELENERNRNFELEMELEFLRLQISPA 520
           +EGEK GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEFLRLQIS A
Sbjct: 492 IEGEKGGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFLRLQISAA 531

BLAST of MC05g0062 vs. ExPASy TrEMBL
Match: A0A6J1IUQ1 (serine/threonine-protein kinase BLUS1 OS=Cucurbita maxima OX=3661 GN=LOC111480108 PE=4 SV=1)

HSP 1 Score: 832 bits (2150), Expect = 6.30e-301
Identity = 436/526 (82.89%), Postives = 476/526 (90.49%), Query Frame = 0

Query: 2   IQSGEVIM---NDQEEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKS 61
           +QSGE+IM   +DQ+E KRPQFPLDSS Y+IIDEIGVGVSAVVYKAICIPMNSAVVAIK+
Sbjct: 12  VQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIKA 71

Query: 62  IDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 121
           IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS
Sbjct: 72  IDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 131

Query: 122 FPDGLPEPCIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFE 181
           FPDGLPEPCIAVVLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIFE
Sbjct: 132 FPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIFE 191

Query: 182 ANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGR 241
           AN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA GR
Sbjct: 192 ANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHGR 251

Query: 242 PPLSHLPPSKSLLLKITRRFRFSDYDNGRNS--KNKFSKGFKELVASCLDQEPSKRPCAQ 301
           PPLSHLPPSKSLLLKITRRFRFSDY+N +NS  KNKFSKGFKELVASCLDQEPS+RPCA 
Sbjct: 252 PPLSHLPPSKSLLLKITRRFRFSDYENQQNSNNKNKFSKGFKELVASCLDQEPSRRPCAD 311

Query: 302 KLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEII 361
           KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQ LPS+K++  E +E E+E+EII
Sbjct: 312 KLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKME--EEDEEEIEKEII 371

Query: 362 KQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGL 421
           KQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG+
Sbjct: 372 KQRRISGWNFKEDGLELEPVFPGENCEEDECSIIKQVRFGGESIM-EKGSRDSSECCSGV 431

Query: 422 SSPSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEKN 481
           S  S    +G R   GGG  EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK 
Sbjct: 432 SDLSSPTGNGRR---GGGEAEEEAVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEKG 491

Query: 482 GEMNREEQMVRLIEKMTQELENERNRNFELEMELEFLRLQISPAYN 522
           GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEFL LQIS A+N
Sbjct: 492 GEMSREEQIGRLMEKMTRELENERKRNFELEMELEFLGLQISAAHN 530

BLAST of MC05g0062 vs. ExPASy TrEMBL
Match: A0A6J1E8I9 (serine/threonine-protein kinase BLUS1 OS=Cucurbita moschata OX=3662 GN=LOC111431839 PE=4 SV=1)

HSP 1 Score: 826 bits (2134), Expect = 1.78e-298
Identity = 436/527 (82.73%), Postives = 476/527 (90.32%), Query Frame = 0

Query: 2   IQSGEVIM---NDQEEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKS 61
           +QSGE+IM   +DQ+E KRPQFPLDSS Y+IIDEIGVGVSAVVYKAICIPMNSAVVAIK+
Sbjct: 12  VQSGELIMKFPDDQDESKRPQFPLDSSCYKIIDEIGVGVSAVVYKAICIPMNSAVVAIKA 71

Query: 62  IDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 121
           IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS
Sbjct: 72  IDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 131

Query: 122 FPDGLPEPCIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFE 181
           FPDGLPEPCIAVVLREIL AL YLHDQGHLHRDIKAGNILMDSNGSVKLADFGVS SIFE
Sbjct: 132 FPDGLPEPCIAVVLREILHALTYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSASIFE 191

Query: 182 ANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGR 241
           AN+ QG+  S  S SMM L ++AGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA GR
Sbjct: 192 ANSTQGVPISPSSTSMM-LKEIAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHGR 251

Query: 242 PPLSHLPPSKSLLLKITRRFRFSDYDNGR--NSKNKFSKGFKELVASCLDQEPSKRPCAQ 301
           PPLSHLPPSKSLLLKITRRFRFSDY+N +  N+KNKFSKGFKELVASCLDQEPS+RPCA 
Sbjct: 252 PPLSHLPPSKSLLLKITRRFRFSDYENQQSSNNKNKFSKGFKELVASCLDQEPSRRPCAD 311

Query: 302 KLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEII 361
           KLLKH FFRNCKGPD+L++TVLQGLPSVEERFKLGKLQ LPS+K +  E +E E+E+EII
Sbjct: 312 KLLKHPFFRNCKGPDLLIKTVLQGLPSVEERFKLGKLQRLPSMKTE--EEDEEEIEKEII 371

Query: 362 KQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGL 421
           KQRRISGWNF EDGLEL+PVFPGENCEEDECSI+KQVRFGGES++ EKGS DSSECCSG+
Sbjct: 372 KQRRISGWNFKEDGLELEPVFPGENCEEDECSIMKQVRFGGESIM-EKGSRDSSECCSGV 431

Query: 422 SS-PSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 481
           S   S TGN    R GG    EEE VD+E+I+GGL+ALKRSLDEQRR VG+LIE +EGEK
Sbjct: 432 SDLSSPTGNG---RRGGVEEEEEEGVDRESIMGGLVALKRSLDEQRRAVGSLIELIEGEK 491

Query: 482 NGEMNREEQMVRLIEKMTQELENERNRNFELEMELEFLRLQISPAYN 522
            GEM+REEQ+ RL+EKMT+ELENER RNFELEMELEF+RLQIS A+N
Sbjct: 492 GGEMSREEQIGRLMEKMTRELENERKRNFELEMELEFVRLQISAAHN 531

BLAST of MC05g0062 vs. ExPASy TrEMBL
Match: A0A5A7U0A0 (Serine/threonine-protein kinase BLUS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001270 PE=4 SV=1)

HSP 1 Score: 814 bits (2103), Expect = 7.45e-294
Identity = 428/527 (81.21%), Postives = 469/527 (88.99%), Query Frame = 0

Query: 2   IQSGEVIM---NDQEEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKS 61
           +QSGE++M   +D ++ KRPQFPLDS+SY+IIDEIGVGVSAVVYKAICIPMNS VVAIK+
Sbjct: 12  VQSGELVMKLTDDHDDSKRPQFPLDSTSYKIIDEIGVGVSAVVYKAICIPMNSVVVAIKA 71

Query: 62  IDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 121
           IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS
Sbjct: 72  IDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 131

Query: 122 FPDGLPEPCIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFE 181
           FPDGLPEPCIA+VLREIL AL+YLHDQGHLHRDIKAGNIL+DSNGS+KLADFGVSTSIFE
Sbjct: 132 FPDGLPEPCIAIVLREILHALSYLHDQGHLHRDIKAGNILIDSNGSIKLADFGVSTSIFE 191

Query: 182 ANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGR 241
           +N+KQG+S S LS SMM L +VAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA GR
Sbjct: 192 SNSKQGVSISPLSTSMM-LKEVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHGR 251

Query: 242 PPLSHLPPSKSLLLKITRRFRFSDYDNGRNSKNK--FSKGFKELVASCLDQEPSKRPCAQ 301
           PPLSHLPPSKSLLLKITRRFRFSDY+N  NSKNK  FSKGFKELVASCLD EPS+RPCA+
Sbjct: 252 PPLSHLPPSKSLLLKITRRFRFSDYENHHNSKNKNKFSKGFKELVASCLDPEPSRRPCAE 311

Query: 302 KLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEII 361
           KLLKH FF+N KGPD+LV+T+LQGLPSVEERFKLGKLQ LPS+K +  E EE ELE+EII
Sbjct: 312 KLLKHPFFKNSKGPDLLVKTILQGLPSVEERFKLGKLQRLPSMKTE--EEEEEELEKEII 371

Query: 362 KQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGL 421
           KQRRISGWNFNEDGLELDPVFPGE C                S++DEKGSGDSSECCSGL
Sbjct: 372 KQRRISGWNFNEDGLELDPVFPGEEC----------------SIMDEKGSGDSSECCSGL 431

Query: 422 SS-PSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 481
           S   S  GN+  R  GGGGGGEEE+VD+E+IVGGLMALK+SLDEQRR VG+LIE +EGEK
Sbjct: 432 SDLSSPVGNE--RGGGGGGGGEEEVVDRESIVGGLMALKKSLDEQRRAVGSLIELMEGEK 491

Query: 482 NGEMNREEQMVRLIEKMTQELENERNRNFELEMELEFLRLQISPAYN 522
            GEM+REEQM RL+EKMT+ELENER RNFELEMELEFLRLQIS A+N
Sbjct: 492 GGEMSREEQMGRLMEKMTRELENERRRNFELEMELEFLRLQISAAHN 517

BLAST of MC05g0062 vs. ExPASy TrEMBL
Match: A0A1S3AXC3 (serine/threonine-protein kinase BLUS1 OS=Cucumis melo OX=3656 GN=LOC103483627 PE=4 SV=1)

HSP 1 Score: 813 bits (2099), Expect = 3.03e-293
Identity = 427/527 (81.02%), Postives = 469/527 (88.99%), Query Frame = 0

Query: 2   IQSGEVIM---NDQEEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKS 61
           +QSGE++M   +D ++ KRPQFPLDS+SY+IIDEIGVGVSAVVYKAICIPMNS VVAIK+
Sbjct: 12  VQSGELVMKLTDDHDDSKRPQFPLDSTSYKIIDEIGVGVSAVVYKAICIPMNSVVVAIKA 71

Query: 62  IDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 121
           IDLDRSRADFDDIRREA+ILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS
Sbjct: 72  IDLDRSRADFDDIRREAKILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 131

Query: 122 FPDGLPEPCIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFE 181
           FPDGLPEPCIA+VLREIL AL+YLHDQGHLHRDIKAGNIL+DSNGS+KLADFGVSTSIFE
Sbjct: 132 FPDGLPEPCIAIVLREILHALSYLHDQGHLHRDIKAGNILIDSNGSIKLADFGVSTSIFE 191

Query: 182 ANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGR 241
           +N+KQG+S S LS SMM L +VAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA GR
Sbjct: 192 SNSKQGVSISPLSTSMM-LKEVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHGR 251

Query: 242 PPLSHLPPSKSLLLKITRRFRFSDYDNGRNSKNK--FSKGFKELVASCLDQEPSKRPCAQ 301
           PPLSHLPPSKSLLLKITRRFRFSDY+N  NSKNK  FSKGFKELVASCLD EPS+RPCA+
Sbjct: 252 PPLSHLPPSKSLLLKITRRFRFSDYENHHNSKNKNKFSKGFKELVASCLDPEPSRRPCAE 311

Query: 302 KLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEII 361
           KLLKH FF+N KGPD+LV+T+LQGLPSVEERFKLGKLQ LPS+K +  E EE ELE+EII
Sbjct: 312 KLLKHPFFKNSKGPDLLVKTILQGLPSVEERFKLGKLQRLPSMKTE--EEEEEELEKEII 371

Query: 362 KQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGL 421
           KQRRISGWNFNEDGLELDPVFPGE C                S+++EKGSGDSSECCSGL
Sbjct: 372 KQRRISGWNFNEDGLELDPVFPGEEC----------------SIMEEKGSGDSSECCSGL 431

Query: 422 SS-PSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 481
           S   S  GN+  R  GGGGGGEEE+VD+E+IVGGLMALK+SLDEQRR VG+LIE +EGEK
Sbjct: 432 SDLSSPVGNE--RGGGGGGGGEEEVVDRESIVGGLMALKKSLDEQRRAVGSLIELMEGEK 491

Query: 482 NGEMNREEQMVRLIEKMTQELENERNRNFELEMELEFLRLQISPAYN 522
            GEM+REEQM RL+EKMT+ELENER RNFELEMELEFLRLQIS A+N
Sbjct: 492 GGEMSREEQMGRLMEKMTRELENERRRNFELEMELEFLRLQISAAHN 517

BLAST of MC05g0062 vs. ExPASy TrEMBL
Match: A0A0A0L8E0 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G141880 PE=4 SV=1)

HSP 1 Score: 802 bits (2072), Expect = 4.19e-289
Identity = 422/527 (80.08%), Postives = 468/527 (88.80%), Query Frame = 0

Query: 2   IQSGEVIM---NDQEEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKS 61
           +QSG++IM   +D ++ +RPQFPLDS+SY+IIDEIGVGVSAVVYKAICIPMNSAVVAIK+
Sbjct: 12  VQSGDLIMKLPDDHDDSRRPQFPLDSTSYKIIDEIGVGVSAVVYKAICIPMNSAVVAIKA 71

Query: 62  IDLDRSRADFDDIRREAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSS 121
           IDLDRSRADFDDIRREA+ILSLLSHPNILN+HCSFTVDRRLWVVMPFMSAGSLQSIITSS
Sbjct: 72  IDLDRSRADFDDIRREAKILSLLSHPNILNSHCSFTVDRRLWVVMPFMSAGSLQSIITSS 131

Query: 122 FPDGLPEPCIAVVLREILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFE 181
           FPDGLPEPCIA+VLREIL AL+YLHDQGHLHRDIKAGNIL+DSNGS+KLADFGVSTSIFE
Sbjct: 132 FPDGLPEPCIAIVLREILHALSYLHDQGHLHRDIKAGNILIDSNGSIKLADFGVSTSIFE 191

Query: 182 ANAKQGLSTSSLSASMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGR 241
           +N+KQG+S S LS S+M L +VAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELA GR
Sbjct: 192 SNSKQGVSISPLSTSIM-LKEVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELAHGR 251

Query: 242 PPLSHLPPSKSLLLKITRRFRFSDYDNGRNSKNK--FSKGFKELVASCLDQEPSKRPCAQ 301
           PPLSHLPPSKSLLLKITRRFRFSDY+N  NSKNK  FSKGFKELVASCLD EPS+RPCA+
Sbjct: 252 PPLSHLPPSKSLLLKITRRFRFSDYENHHNSKNKNKFSKGFKELVASCLDPEPSRRPCAE 311

Query: 302 KLLKHSFFRNCKGPDVLVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEII 361
           KLLKH FF+N KGPD+LV+T+LQGLPSVEERFKLGKLQ LPS+K +  E EE ELE+EII
Sbjct: 312 KLLKHPFFKNSKGPDLLVKTILQGLPSVEERFKLGKLQRLPSMKTE--EEEEEELEKEII 371

Query: 362 KQRRISGWNFNEDGLELDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGL 421
           KQRRISGWNFNEDGLELDPVFPGE C                S+++EKGSG+SSECCSGL
Sbjct: 372 KQRRISGWNFNEDGLELDPVFPGEEC----------------SIMEEKGSGNSSECCSGL 431

Query: 422 SS-PSQTGNDGDRRSGGGGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEK 481
           S   S  GN+    SGGGGG EEE+VD+E+IVGGLMALKRSLDEQRR VG+LIE +EGEK
Sbjct: 432 SDLSSPVGNERGGGSGGGGGEEEEVVDRESIVGGLMALKRSLDEQRRAVGSLIELMEGEK 491

Query: 482 NGEMNREEQMVRLIEKMTQELENERNRNFELEMELEFLRLQISPAYN 522
            GEM+REEQM RL+EK+T+ELENER RNFELEMELEFLRLQIS  +N
Sbjct: 492 VGEMSREEQMGRLMEKVTRELENERRRNFELEMELEFLRLQISAFHN 519

BLAST of MC05g0062 vs. TAIR 10
Match: AT4G14480.1 (Protein kinase superfamily protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.6e-145
Identity = 278/497 (55.94%), Postives = 350/497 (70.42%), Query Frame = 0

Query: 19  QFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIRREAQIL 78
           +FPLD+ +Y II +IGVGVSA VYKAICIPMNS VVAIK+IDLD+SRADFD +RRE + +
Sbjct: 7   EFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLDQSRADFDSLRRETKTM 66

Query: 79  SLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAVVLREILSA 138
           SLLSHPNILNA+CSFTVDR LWVVMPFMS GSL SI++SSFP GLPE CI+V L+E L+A
Sbjct: 67  SLLSHPNILNAYCSFTVDRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKETLNA 126

Query: 139 LAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMMKLT 198
           ++YLHDQGHLHRDIKAGNIL+DS+GSVKLADFGVS SI+E       S+S  ++S ++LT
Sbjct: 127 ISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVT----SSSGTTSSSLRLT 186

Query: 199 DVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKITRRF 258
           D+AGTPYWMAPEV+HSH GY  KADIWSFGITALELA GRPPLSHLPP KSLL+KIT+RF
Sbjct: 187 DIAGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKRF 246

Query: 259 RFSDYD-----NGRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVL 318
            FSDY+     + +    KFSK F+E+V  CL+Q+P+KRP A+KLLKH FF+NCKG D +
Sbjct: 247 HFSDYEINTSGSSKKGNKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKGLDFV 306

Query: 319 VRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELE-EEIIKQRRISGWNFNEDGLE 378
           V+ VL  L + E+ F   ++     +K  GD+ EE E E EEI+K RRISGWNF ED L+
Sbjct: 307 VKNVLHSLSNAEQMFMESQI----LIKSVGDDDEEEEEEDEEIVKNRRISGWNFREDDLQ 366

Query: 379 LDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSECCSGLSSPSQTGNDGDRRSGG 438
           L PVFP    +  E            S  +E  S D  E  +   +  + G         
Sbjct: 367 LSPVFPATESDSSE-----------SSPREEDQSKDKKEDDNVTITGYELGLGLSNEEAK 426

Query: 439 GGGGEEEIVDKEAIVGGLMALKRSLDEQRRTVGNLIEFLEGEKNGEMNREEQMVRLIEKM 498
              GE    DK+ ++  L  LK+SL+ QR  V  +IE L G+K  E +REE+++ ++EK+
Sbjct: 427 NQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKE-EKSREEELLEMVEKL 483

Query: 499 TQELENERNRNFELEME 510
             ELE E+ +    + +
Sbjct: 487 KIELETEKLKTLRADKD 483

BLAST of MC05g0062 vs. TAIR 10
Match: AT5G14720.1 (Protein kinase superfamily protein )

HSP 1 Score: 344.0 bits (881), Expect = 2.2e-94
Identity = 186/410 (45.37%), Postives = 253/410 (61.71%), Query Frame = 0

Query: 13  EEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIR 72
           E     +FPL++  Y++ +EIG GVSA V++A+CIP+N  VVAIK +DL++   D D IR
Sbjct: 2   ESGSEKKFPLNAKDYKLYEEIGDGVSATVHRALCIPLN-VVVAIKVLDLEKCNNDLDGIR 61

Query: 73  REAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAVVL 132
           RE Q +SL++HPN+L AHCSFT   +LWVVMP+M+ GS   II SS+PDG  EP IA +L
Sbjct: 62  REVQTMSLINHPNVLQAHCSFTTGHQLWVVMPYMAGGSCLHIIKSSYPDGFEEPVIATLL 121

Query: 133 REILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSA 192
           RE L AL YLH  GH+HRD+KAGNIL+DSNG+VKLADFGVS  +F+   +Q         
Sbjct: 122 RETLKALVYLHAHGHIHRDVKAGNILLDSNGAVKLADFGVSACMFDTGDRQ--------- 181

Query: 193 SMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLL 252
                    GTP WMAPEV+   +GY  KAD+WSFGITALELA G  P S  PP K LL+
Sbjct: 182 --RSRNTFVGTPCWMAPEVMQQLHGYDFKADVWSFGITALELAHGHAPFSKYPPMKVLLM 241

Query: 253 KITRRFRFSDYDNGRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDV 312
            +       DY+  +    +FSK FKE+V +CL ++P KRP ++KLLKH FF++ +  D 
Sbjct: 242 TLQNAPPGLDYERDK----RFSKAFKEMVGTCLVKDPKKRPTSEKLLKHPFFKHARPADY 301

Query: 313 LVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEIIKQRRISGWNFNEDGLE 372
           LV+T+L GLP + +R++  K +    +  +  E E    ++E I  R IS WNFN + L+
Sbjct: 302 LVKTILNGLPPLGDRYRQIKSKEADLLMQNKSEYEAHLSQQEYI--RGISAWNFNLEDLK 361

Query: 373 LDPVFPGENCEEDECSIVKQVRFGGESVIDEKGSGDSSE-CCSGLSSPSQ 422
                      +D+ S  ++  F  +    +  S  S E   S  ++PSQ
Sbjct: 362 TQAAL----ISDDDTSHAEEPDFNQKQCERQDESALSPERASSSATAPSQ 389

BLAST of MC05g0062 vs. TAIR 10
Match: AT4G10730.1 (Protein kinase superfamily protein )

HSP 1 Score: 325.1 bits (832), Expect = 1.0e-88
Identity = 166/360 (46.11%), Postives = 229/360 (63.61%), Query Frame = 0

Query: 13  EEPKRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIR 72
           ++ K+  F ++   Y++++E+G G SAVV++AI +P N  VVAIKS+DLDR  ++ DDIR
Sbjct: 33  DKKKKKGFSVNPKDYKLMEEVGYGASAVVHRAIYLPTNE-VVAIKSLDLDRCNSNLDDIR 92

Query: 73  REAQILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAVVL 132
           REAQ ++L+ HPN++ + CSF VD  LWVVMPFM+ GS   ++ +++PDG  E  I  +L
Sbjct: 93  REAQTMTLIDHPNVIKSFCSFAVDHHLWVVMPFMAQGSCLHLMKAAYPDGFEEAAICSML 152

Query: 133 REILSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSA 192
           +E L AL YLH QGH+HRD+KAGNIL+D  G +KL DFGVS  +F+   +Q    +    
Sbjct: 153 KETLKALDYLHRQGHIHRDVKAGNILLDDTGEIKLGDFGVSACLFDNGDRQRARNT---- 212

Query: 193 SMMKLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLL 252
                    GTP WMAPEV+   +GY+ KADIWSFGITALELA G  P S  PP K LL+
Sbjct: 213 -------FVGTPCWMAPEVLQPGSGYNSKADIWSFGITALELAHGHAPFSKYPPMKVLLM 272

Query: 253 KITRRFRFSDYDNGRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDV 312
            I       DYD  +    KFSK FKELVA CL ++ +KRP A+KLLKHSFF+N K P++
Sbjct: 273 TIQNAPPGLDYDRDK----KFSKSFKELVALCLVKDQTKRPTAEKLLKHSFFKNVKPPEI 332

Query: 313 LVRTVLQGLPSVEERFKLGKLQHLPSVKIDGDEGEEGELEEEIIKQRRISGWNFNEDGLE 372
            V+ +   LP +  R K  + +    + + G    + +   +   QR +S WNFN + L+
Sbjct: 333 CVKKLFVDLPPLWTRVKALQAKDAAQLALKGMASADQDAISQSEYQRGVSAWNFNIEDLK 376

BLAST of MC05g0062 vs. TAIR 10
Match: AT1G79640.1 (Protein kinase superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 2.2e-86
Identity = 162/358 (45.25%), Postives = 226/358 (63.13%), Query Frame = 0

Query: 16  KRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIRREA 75
           ++ ++P+    Y + + IG GVSA+V++A+CIP +  VVAIK +D +R   D ++I REA
Sbjct: 5   EKKKYPIGPEHYTLYEFIGQGVSALVHRALCIPFDE-VVAIKILDFERDNCDLNNISREA 64

Query: 76  QILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAVVLREI 135
           Q + L+ HPN+L +HCSF  D  LWV+MP+MS GS   I+ +++PDG  E  IA +LRE 
Sbjct: 65  QTMMLVDHPNVLKSHCSFVSDHNLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREA 124

Query: 136 LSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMM 195
           L  L YLH  GH+HRD+KAGNIL+ + G+VKL DFGVS  +F++  +Q    +       
Sbjct: 125 LKGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNT------- 184

Query: 196 KLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKIT 255
                 GTP WMAPEV+   +GY  KADIWSFGIT LELA G  P S  PP K LL+ + 
Sbjct: 185 ----FVGTPCWMAPEVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQ 244

Query: 256 RRFRFSDYDNGRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVLVR 315
                 DY+  +    KFS+ FK+++ASCL ++PSKRP A+KLLKHSFF+  +  D + R
Sbjct: 245 NAPPGLDYERDK----KFSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQARSSDYIAR 304

Query: 316 TVLQGLPSVEERFKLGKLQHLPSVKIDG-DEGEEGELEEEIIKQRRISGWNFNEDGLE 373
            +L GLP +  R +  K +    +  +   +GE+ EL +   K R ISGWNFN D ++
Sbjct: 305 KLLDGLPDLVNRVQAIKRKEEDMLAQEKMADGEKEELSQNEYK-RGISGWNFNLDDMK 345

BLAST of MC05g0062 vs. TAIR 10
Match: AT1G79640.2 (Protein kinase superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 2.2e-86
Identity = 162/358 (45.25%), Postives = 226/358 (63.13%), Query Frame = 0

Query: 16  KRPQFPLDSSSYRIIDEIGVGVSAVVYKAICIPMNSAVVAIKSIDLDRSRADFDDIRREA 75
           ++ ++P+    Y + + IG GVSA+V++A+CIP +  VVAIK +D +R   D ++I REA
Sbjct: 2   EKKKYPIGPEHYTLYEFIGQGVSALVHRALCIPFDE-VVAIKILDFERDNCDLNNISREA 61

Query: 76  QILSLLSHPNILNAHCSFTVDRRLWVVMPFMSAGSLQSIITSSFPDGLPEPCIAVVLREI 135
           Q + L+ HPN+L +HCSF  D  LWV+MP+MS GS   I+ +++PDG  E  IA +LRE 
Sbjct: 62  QTMMLVDHPNVLKSHCSFVSDHNLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREA 121

Query: 136 LSALAYLHDQGHLHRDIKAGNILMDSNGSVKLADFGVSTSIFEANAKQGLSTSSLSASMM 195
           L  L YLH  GH+HRD+KAGNIL+ + G+VKL DFGVS  +F++  +Q    +       
Sbjct: 122 LKGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNT------- 181

Query: 196 KLTDVAGTPYWMAPEVIHSHNGYSLKADIWSFGITALELADGRPPLSHLPPSKSLLLKIT 255
                 GTP WMAPEV+   +GY  KADIWSFGIT LELA G  P S  PP K LL+ + 
Sbjct: 182 ----FVGTPCWMAPEVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQ 241

Query: 256 RRFRFSDYDNGRNSKNKFSKGFKELVASCLDQEPSKRPCAQKLLKHSFFRNCKGPDVLVR 315
                 DY+  +    KFS+ FK+++ASCL ++PSKRP A+KLLKHSFF+  +  D + R
Sbjct: 242 NAPPGLDYERDK----KFSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQARSSDYIAR 301

Query: 316 TVLQGLPSVEERFKLGKLQHLPSVKIDG-DEGEEGELEEEIIKQRRISGWNFNEDGLE 373
            +L GLP +  R +  K +    +  +   +GE+ EL +   K R ISGWNFN D ++
Sbjct: 302 KLLDGLPDLVNRVQAIKRKEEDMLAQEKMADGEKEELSQNEYK-RGISGWNFNLDDMK 342