MC05g0057 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0057
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionInhibitor against trypsin
LocationMC05: 414955 .. 415150 (+)
RNA-Seq ExpressionMC05g0057
SyntenyMC05g0057
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTCAAGGAAAGAGCTCATGGCCGCGGCTGGTGGGATCGACCAGCGCGGCGGCGAAGGCGGTCATCGAGAGGGAGAATCCTCGCGTCAGAGCCGTCATTATAAAGGTCGGAGCCCGCCACCCTGGACTTCCGCTGCGATCGGGTTCGGGTTTGGGTCACTGAGAGGGGAATCGTTGCAAGGCCACCCACAATTGGC

mRNA sequence

TGTCAAGGAAAGAGCTCATGGCCGCGGCTGGTGGGATCGACCAGCGCGGCGGCGAAGGCGGTCATCGAGAGGGAGAATCCTCGCGTCAGAGCCGTCATTATAAAGGTCGGAGCCGCCACCCTGGACTTCCGCTGCGATCGGGTTCGGGTTTGGGTCACTGAGAGGGGAATCGTTGCAAGGCCACCCACAATTGGC

Coding sequence (CDS)

TGTCAAGGAAAGAGCTCATGGCCGCGGCTGGTGGGATCGACCAGCGCGGCGGCGAAGGCGGTCATCGAGAGGGAGAATCCTCGCGTCAGAGCCGTCATTATAAAGGTCGGAGCCGCCACCCTGGACTTCCGCTGCGATCGGGTTCGGGTTTGGGTCACTGAGAGGGGAATCGTTGCAAGGCCACCCACAATTGGC

Protein sequence

CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGAATLDFRCDRVRVWVTERGIVARPPTIG
Homology
BLAST of MC05g0057 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 2.2e-25
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          CQGK SWP+LVGST AAAKAVIERENPRVRAVI++VG+  T DFRCDRVRVWVTERGIVA
Sbjct: 3  CQGKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVA 62

Query: 61 RPPTIG 66
          RPP IG
Sbjct: 63 RPPAIG 68

BLAST of MC05g0057 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 4.9e-17
Identity = 40/66 (60.61%), Postives = 50/66 (75.76%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          C GKSSWP LVG   + AKA+IER+NP V+AVI++ G   T DFRC+RVR+WV +RG+V 
Sbjct: 3  CPGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVV 62

Query: 61 RPPTIG 66
           PP IG
Sbjct: 63 SPPRIG 68

BLAST of MC05g0057 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 5.2e-11
Identity = 33/65 (50.77%), Postives = 43/65 (66.15%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGAA-TLDFRCDRVRVWVTERGIVA 60
          C GK++WP LVG +   A A +EREN  V A+++K G+A T DFRCDRV V V + G+V 
Sbjct: 4  CPGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVT 63

Query: 61 RPPTI 65
            P I
Sbjct: 64 SVPHI 68

BLAST of MC05g0057 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 7.5e-10
Identity = 32/65 (49.23%), Postives = 39/65 (60.00%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVI-IKVGAATLDFRCDRVRVWVTERGIVA 60
          C GK  WP LVG     A A+IERENP VR+++  +    T DFRCDRV V V   G+V 
Sbjct: 4  CPGKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGVVV 63

Query: 61 RPPTI 65
          R P +
Sbjct: 64 RTPRV 68

BLAST of MC05g0057 vs. ExPASy Swiss-Prot
Match: P09407 (Trypsin inhibitor MCI-3 OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.9e-08
Identity = 33/55 (60.00%), Postives = 35/55 (63.64%), Query Frame = 0

Query: 11 VGSTSAAAKAVIERENPRVRAVIIKVGAATLDFRCDRVRVWVTERGIVARPPTIG 66
          VGST AAAKA++ERENPRVRA                   WVTERGIVARPPTIG
Sbjct: 27 VGSTGAAAKALVERENPRVRA-------------------WVTERGIVARPPTIG 62

BLAST of MC05g0057 vs. NCBI nr
Match: BAB32588.1 (inhibitor against trypsin, partial [Momordica charantia])

HSP 1 Score: 122 bits (307), Expect = 5.74e-35
Identity = 61/66 (92.42%), Postives = 63/66 (95.45%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          CQGKSSWP+LVGST AAAKAVIERENPRVRAVIIKVG+ AT DFRCDRVRVWVTERGIVA
Sbjct: 1  CQGKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVA 60

Query: 61 RPPTIG 65
          RPPTIG
Sbjct: 61 RPPTIG 66

BLAST of MC05g0057 vs. NCBI nr
Match: 1VBW_A (Crystal Structure of Bitter Gourd Trypsin Inhibitor [Momordica charantia] >prf||2111250B trypsin inhibitor BGIT [Momordica charantia])

HSP 1 Score: 122 bits (307), Expect = 6.06e-35
Identity = 61/66 (92.42%), Postives = 63/66 (95.45%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          CQGKSSWP+LVGST AAAKAVIERENPRVRAVIIKVG+ AT DFRCDRVRVWVTERGIVA
Sbjct: 3  CQGKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVA 62

Query: 61 RPPTIG 65
          RPPTIG
Sbjct: 63 RPPTIG 68

BLAST of MC05g0057 vs. NCBI nr
Match: P24076.1 (RecName: Full=Glu S.griseus protease inhibitor; AltName: Full=BGIA [Momordica charantia] >AAB19651.1 BGIA=acidic amino acid-specific endopeptidase inhibitor [Momordica charantia L.=bitter gourd, Peptide, 68 aa] [Momordica charantia])

HSP 1 Score: 116 bits (290), Expect = 2.39e-32
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          CQGK SWP+LVGST AAAKAVIERENPRVRAVI++VG+  T DFRCDRVRVWVTERGIVA
Sbjct: 3  CQGKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVA 62

Query: 61 RPPTIG 65
          RPP IG
Sbjct: 63 RPPAIG 68

BLAST of MC05g0057 vs. NCBI nr
Match: KAA0049292.1 (trypsin inhibitor [Cucumis melo var. makuwa] >TYK17266.1 trypsin inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 95.9 bits (237), Expect = 3.00e-24
Identity = 44/66 (66.67%), Postives = 53/66 (80.30%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          CQGK SWP+LVG   + AKA+IERENP VRAV+++VG   T DFRC+RVRVWV + G+VA
Sbjct: 4  CQGKRSWPQLVGVGGSVAKAIIERENPNVRAVVLEVGTPVTKDFRCNRVRVWVNKNGLVA 63

Query: 61 RPPTIG 65
           PPTIG
Sbjct: 64 SPPTIG 69

BLAST of MC05g0057 vs. NCBI nr
Match: XP_022946028.1 (inhibitor of trypsin and hageman factor [Cucurbita moschata])

HSP 1 Score: 92.8 bits (229), Expect = 4.98e-23
Identity = 43/66 (65.15%), Postives = 52/66 (78.79%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          C GKSSWP LVG   +AAKA+IERENP V+AVI++ G   T DFRC+RVR+WV +RG+VA
Sbjct: 4  CPGKSSWPHLVGVGGSAAKAIIERENPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVA 63

Query: 61 RPPTIG 65
           PP IG
Sbjct: 64 SPPRIG 69

BLAST of MC05g0057 vs. ExPASy TrEMBL
Match: Q9AYN0 (Inhibitor against trypsin (Fragment) OS=Momordica charantia OX=3673 GN=bgit PE=2 SV=1)

HSP 1 Score: 122 bits (307), Expect = 2.78e-35
Identity = 61/66 (92.42%), Postives = 63/66 (95.45%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          CQGKSSWP+LVGST AAAKAVIERENPRVRAVIIKVG+ AT DFRCDRVRVWVTERGIVA
Sbjct: 1  CQGKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVA 60

Query: 61 RPPTIG 65
          RPPTIG
Sbjct: 61 RPPTIG 66

BLAST of MC05g0057 vs. ExPASy TrEMBL
Match: Q7M1Q1 (Trypsin inhibitor BGIT OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 122 bits (307), Expect = 2.94e-35
Identity = 61/66 (92.42%), Postives = 63/66 (95.45%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          CQGKSSWP+LVGST AAAKAVIERENPRVRAVIIKVG+ AT DFRCDRVRVWVTERGIVA
Sbjct: 3  CQGKSSWPQLVGSTGAAAKAVIERENPRVRAVIIKVGSGATKDFRCDRVRVWVTERGIVA 62

Query: 61 RPPTIG 65
          RPPTIG
Sbjct: 63 RPPTIG 68

BLAST of MC05g0057 vs. ExPASy TrEMBL
Match: A0A5A7U0I2 (Trypsin inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001190 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.45e-24
Identity = 44/66 (66.67%), Postives = 53/66 (80.30%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          CQGK SWP+LVG   + AKA+IERENP VRAV+++VG   T DFRC+RVRVWV + G+VA
Sbjct: 4  CQGKRSWPQLVGVGGSVAKAIIERENPNVRAVVLEVGTPVTKDFRCNRVRVWVNKNGLVA 63

Query: 61 RPPTIG 65
           PPTIG
Sbjct: 64 SPPTIG 69

BLAST of MC05g0057 vs. ExPASy TrEMBL
Match: A0A6J1G2N1 (inhibitor of trypsin and hageman factor OS=Cucurbita moschata OX=3662 GN=LOC111450237 PE=3 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.41e-23
Identity = 43/66 (65.15%), Postives = 52/66 (78.79%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          C GKSSWP LVG   +AAKA+IERENP V+AVI++ G   T DFRC+RVR+WV +RG+VA
Sbjct: 4  CPGKSSWPHLVGVGGSAAKAIIERENPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVA 63

Query: 61 RPPTIG 65
           PP IG
Sbjct: 64 SPPRIG 69

BLAST of MC05g0057 vs. ExPASy TrEMBL
Match: A0A251VNK4 (Putative serine protease inhibitor, potato inhibitor I-type family protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr01g0014531 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.88e-22
Identity = 42/66 (63.64%), Postives = 49/66 (74.24%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          C+GKSSWP LVG+   AA A IERENPRV A++I+ G+  T DFRCDRVRVWV    +V 
Sbjct: 15 CEGKSSWPELVGARGDAAAATIERENPRVDAIVIREGSFVTADFRCDRVRVWVNSNRVVV 74

Query: 61 RPPTIG 65
          RPP IG
Sbjct: 75 RPPVIG 80

BLAST of MC05g0057 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 67.4 bits (163), Expect = 4.8e-12
Identity = 35/64 (54.69%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 3  GKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGAATLD-FRCDRVRVWVTERGIVARP 62
          GKSSWP L+G+    AK VIERENP+++AVII  G    + F C RV VWV + GIV + 
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 63 PTIG 66
          P IG
Sbjct: 96 PIIG 99

BLAST of MC05g0057 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 65.1 bits (157), Expect = 2.4e-11
Identity = 34/66 (51.52%), Postives = 40/66 (60.61%), Query Frame = 0

Query: 1  CQGKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGA-ATLDFRCDRVRVWVTERGIVA 60
          C  K+SWP L G+    A  VIERENP V A +I  G+  T DFRCDRVRV+V    IV 
Sbjct: 5  CPRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVV 64

Query: 61 RPPTIG 66
          + P  G
Sbjct: 65 KTPKSG 70

BLAST of MC05g0057 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 56.6 bits (135), Expect = 8.6e-09
Identity = 30/64 (46.88%), Postives = 38/64 (59.38%), Query Frame = 0

Query: 3  GKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVG-AATLDFRCDRVRVWVTERGIVARP 62
          GK+SWP L+G+    A +VI+ EN  +  V++  G   T D  C RVRVWV E  IV R 
Sbjct: 10 GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 69

Query: 63 PTIG 66
          PT G
Sbjct: 70 PTAG 73

BLAST of MC05g0057 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 56.6 bits (135), Expect = 8.6e-09
Identity = 30/64 (46.88%), Postives = 38/64 (59.38%), Query Frame = 0

Query: 3  GKSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVG-AATLDFRCDRVRVWVTERGIVARP 62
          GK+SWP L+G+    A +VI+ EN  +  V++  G   T D  C RVRVWV E  IV R 
Sbjct: 25 GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 84

Query: 63 PTIG 66
          PT G
Sbjct: 85 PTAG 88

BLAST of MC05g0057 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 54.7 bits (130), Expect = 3.3e-08
Identity = 28/63 (44.44%), Postives = 37/63 (58.73%), Query Frame = 0

Query: 4  KSSWPRLVGSTSAAAKAVIERENPRVRAVIIKVGAATLD-FRCDRVRVWVTERGIVARPP 63
          K  WP LVG     AK  IERENP V A++++ G   ++ F C+RV V++   G VA  P
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAP 82

Query: 64 TIG 66
           IG
Sbjct: 83 MIG 85

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P240762.2e-2584.85Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1[more]
P198734.9e-1760.61Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
P823815.2e-1150.77Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1[more]
P802117.5e-1049.23Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1[more]
P094071.9e-0860.00Trypsin inhibitor MCI-3 OS=Momordica charantia OX=3673 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
BAB32588.15.74e-3592.42inhibitor against trypsin, partial [Momordica charantia][more]
1VBW_A6.06e-3592.42Crystal Structure of Bitter Gourd Trypsin Inhibitor [Momordica charantia] >prf||... [more]
P24076.12.39e-3284.85RecName: Full=Glu S.griseus protease inhibitor; AltName: Full=BGIA [Momordica ch... [more]
KAA0049292.13.00e-2466.67trypsin inhibitor [Cucumis melo var. makuwa] >TYK17266.1 trypsin inhibitor [Cucu... [more]
XP_022946028.14.98e-2365.15inhibitor of trypsin and hageman factor [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9AYN02.78e-3592.42Inhibitor against trypsin (Fragment) OS=Momordica charantia OX=3673 GN=bgit PE=2... [more]
Q7M1Q12.94e-3592.42Trypsin inhibitor BGIT OS=Momordica charantia OX=3673 PE=1 SV=1[more]
A0A5A7U0I21.45e-2466.67Trypsin inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00... [more]
A0A6J1G2N12.41e-2365.15inhibitor of trypsin and hageman factor OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A251VNK41.88e-2263.64Putative serine protease inhibitor, potato inhibitor I-type family protein OS=He... [more]
Match NameE-valueIdentityDescription
AT5G43580.14.8e-1254.69Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38870.12.4e-1151.52Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.18.6e-0946.88Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.28.6e-0946.88Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT3G46860.13.3e-0844.44Serine protease inhibitor, potato inhibitor I-type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRINTSPR00292POTATOINHBTRcoord: 6..19
score: 47.52
coord: 42..52
score: 60.47
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 4..65
e-value: 1.4E-22
score: 80.1
IPR000864Proteinase inhibitor I13, potato inhibitor IPANTHERPTHR33091PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 1..65
NoneNo IPR availableGENE3D3.30.10.10Trypsin Inhibitor V, subunit Acoord: 1..65
e-value: 5.2E-25
score: 89.6
NoneNo IPR availablePANTHERPTHR33091:SF41SERINE PROTEASE INHIBITOR, POTATO INHIBITOR I-TYPE FAMILY PROTEIN-RELATEDcoord: 1..65
IPR036354Proteinase inhibitor I13, potato inhibitor I superfamilySUPERFAMILY54654CI-2 family of serine protease inhibitorscoord: 1..65

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0057.1MC05g0057.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0009611 response to wounding
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity