MC05g0055 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0055
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Proteinase inhibitor
Location	MC05: 409342 .. 409539 (-)
RNA-Seq Expression	MC05g0055
Synteny	MC05g0055

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAAGAGTGCGTGGCCGGAGCTTGTTTGGTCTCCGGCGAAAGCTGCGGCGGCAGCCATCCAGAAAGAAAATCCTTTGGTTAAAAAAGCCATAATTGTGGAAGAAGGTTCTGCGGTGACAACCGACTTCAGGTGCGATCGGGTTTTCATTTTTAAGGAGAAGAATGGTGAGCTTGTTACCAGGACCCCTCACATCGGC

mRNA sequence

GGAAAGAGTGCGTGGCCGGAGCTTGTTTGGTCTCCGGCGAAAGCTGCGGCGGCAGCCATCCAGAAAGAAAATCCTTTGGTTAAAAAAGCCATAATTGTGGAAGAAGGTTCTGCGGTGACAACCGACTTCAGGTGCGATCGGGTTTTCATTTTTAAGGAGAAGAATGGTGAGCTTGTTACCAGGACCCCTCACATCGGC

Coding sequence (CDS)

GGAAAGAGTGCGTGGCCGGAGCTTGTTTGGTCTCCGGCGAAAGCTGCGGCGGCAGCCATCCAGAAAGAAAATCCTTTGGTTAAAAAAGCCATAATTGTGGAAGAAGGTTCTGCGGTGACAACCGACTTCAGGTGCGATCGGGTTTTCATTTTTAAGGAGAAGAATGGTGAGCTTGTTACCAGGACCCCTCACATCGGC

Protein sequence

GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVTRTPHIG

Homology

[Close]

BLAST of MC05g0055 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.0e-10
Identity = 34/65 (52.31%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GK+AWPELV      AAA +++EN  V  AI+++EGSA+T DFRCDRV++    +G +VT
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNV-HAIVLKEGSAMTKDFRCDRVWVIVNDHG-VVT 65

Query: 61 RTPHI 66
            PHI
Sbjct: 66 SVPHI 68

[Close]

BLAST of MC05g0055 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.4e-10
Identity = 35/66 (53.03%), Postives = 45/66 (68.18%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GK +WP+LV S   AA A I++ENP V +A+IV  GS VT DFRCDRV ++  + G +V 
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRV-RAVIVRVGSPVTADFRCDRVRVWVTERG-IVA 64

Query: 61 RTPHIG 67
          R P IG
Sbjct: 65 RPPAIG 68

[Close]

BLAST of MC05g0055 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.3e-09
Identity = 35/66 (53.03%), Postives = 44/66 (66.67%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WP LV      A A I+++NP V KA+I+EEG+ VT DFRC+RV I+  K G LV 
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNV-KAVILEEGTPVTKDFRCNRVRIWVNKRG-LVV 64

Query: 61 RTPHIG 67
            P IG
Sbjct: 65 SPPRIG 68

[Close]

BLAST of MC05g0055 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 5.5e-08
Identity = 31/65 (47.69%), Postives = 41/65 (63.08%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GK  WPELV      AAA I++ENP V ++I+  E S  T DFRCDRV++  +  G +V 
Sbjct: 6  GKQEWPELVGEYGYKAAAIIERENPNV-RSIVKHERSGFTKDFRCDRVWVVVDSTG-VVV 65

Query: 61 RTPHI 66
          RTP +
Sbjct: 66 RTPRV 68

[Close]

BLAST of MC05g0055 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.4e-08
Identity = 29/59 (49.15%), Postives = 36/59 (61.02%), Query Frame = 0


Query: 1   GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELV 60
           GK  WPEL+  PA  A   I+KENP +    I+  GS VT DFRCDRV +F    G++V
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVV 105

[Close]

BLAST of MC05g0055 vs. NCBI nr
Match: KGN56903.1 (hypothetical protein Csa_010041 [Cucumis sativus])

HSP 1 Score: 79.3 bits (194), Expect = 1.19e-17
Identity = 40/66 (60.61%), Postives = 49/66 (74.24%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WPELV +P + A   I+KEN  VK AIIVEEGS+V T+F C RVF+F  K   +VT
Sbjct: 7  GKSSWPELVNAPGEIAVKIIEKENSSVK-AIIVEEGSSVVTNFECGRVFVFIHKKSNMVT 66

Query: 61 RTPHIG 66
          +TPHIG
Sbjct: 67 KTPHIG 71

[Close]

BLAST of MC05g0055 vs. NCBI nr
Match: XP_004134090.1 (glu S.griseus protease inhibitor [Cucumis sativus])

HSP 1 Score: 75.1 bits (183), Expect = 5.65e-16
Identity = 38/66 (57.58%), Postives = 47/66 (71.21%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WPELV    K A   I+KENP V  A+IV+EGS VT DFRCDRV+++ +    +VT
Sbjct: 7  GKSSWPELVGKAGKEAEKIIEKENPSVD-AVIVDEGSVVTQDFRCDRVWVWVDSKTSIVT 66

Query: 61 RTPHIG 66
          RTP IG
Sbjct: 67 RTPFIG 71

[Close]

BLAST of MC05g0055 vs. NCBI nr
Match: KAA0049293.1 (inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK17265.1 inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa])

HSP 1 Score: 73.9 bits (180), Expect = 1.62e-15
Identity = 38/66 (57.58%), Postives = 48/66 (72.73%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WPELV +PA+ A   I+K+N  VK AI+VEEGS+V T+F C RVF+F  K    VT
Sbjct: 7  GKSSWPELVNAPAEIAVKIIEKQNSSVK-AIVVEEGSSVVTNFECGRVFVFVHKKTNNVT 66

Query: 61 RTPHIG 66
          +TP IG
Sbjct: 67 KTPRIG 71

[Close]

BLAST of MC05g0055 vs. NCBI nr
Match: KAA0049295.1 (inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK17263.1 inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa])

HSP 1 Score: 73.9 bits (180), Expect = 1.62e-15
Identity = 38/66 (57.58%), Postives = 47/66 (71.21%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WPELV    K A   I++ENPLV  AIIV+EGS V  DFRCDRV+++ +    +VT
Sbjct: 7  GKSSWPELVGKAGKEAEKTIERENPLVD-AIIVDEGSVVIQDFRCDRVWVWVDPKTAIVT 66

Query: 61 RTPHIG 66
          RTP IG
Sbjct: 67 RTPFIG 71

[Close]

BLAST of MC05g0055 vs. NCBI nr
Match: KAE8650342.1 (hypothetical protein Csa_009611 [Cucumis sativus])

HSP 1 Score: 75.1 bits (183), Expect = 2.91e-15
Identity = 38/66 (57.58%), Postives = 47/66 (71.21%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WPELV    K A   I+KENP V  A+IV+EGS VT DFRCDRV+++ +    +VT
Sbjct: 7  GKSSWPELVGKAGKEAEKIIEKENPSVD-AVIVDEGSVVTQDFRCDRVWVWVDSKTSIVT 66

Query: 61 RTPHIG 66
          RTP IG
Sbjct: 67 RTPFIG 71

[Close]

BLAST of MC05g0055 vs. ExPASy TrEMBL
Match: A0A0A0L593 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142950 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 5.76e-18
Identity = 40/66 (60.61%), Postives = 49/66 (74.24%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WPELV +P + A   I+KEN  VK AIIVEEGS+V T+F C RVF+F  K   +VT
Sbjct: 7  GKSSWPELVNAPGEIAVKIIEKENSSVK-AIIVEEGSSVVTNFECGRVFVFIHKKSNMVT 66

Query: 61 RTPHIG 66
          +TPHIG
Sbjct: 67 KTPHIG 71

[Close]

BLAST of MC05g0055 vs. ExPASy TrEMBL
Match: A0A0A0L795 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142960 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.74e-16
Identity = 38/66 (57.58%), Postives = 47/66 (71.21%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WPELV    K A   I+KENP V  A+IV+EGS VT DFRCDRV+++ +    +VT
Sbjct: 7  GKSSWPELVGKAGKEAEKIIEKENPSVD-AVIVDEGSVVTQDFRCDRVWVWVDSKTSIVT 66

Query: 61 RTPHIG 66
          RTP IG
Sbjct: 67 RTPFIG 71

[Close]

BLAST of MC05g0055 vs. ExPASy TrEMBL
Match: A0A5D3D0M4 (Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001180 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 7.84e-16
Identity = 38/66 (57.58%), Postives = 48/66 (72.73%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WPELV +PA+ A   I+K+N  VK AI+VEEGS+V T+F C RVF+F  K    VT
Sbjct: 7  GKSSWPELVNAPAEIAVKIIEKQNSSVK-AIVVEEGSSVVTNFECGRVFVFVHKKTNNVT 66

Query: 61 RTPHIG 66
          +TP IG
Sbjct: 67 KTPRIG 71

[Close]

BLAST of MC05g0055 vs. ExPASy TrEMBL
Match: A0A5D3CZE2 (Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001160 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 7.84e-16
Identity = 38/66 (57.58%), Postives = 47/66 (71.21%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WPELV    K A   I++ENPLV  AIIV+EGS V  DFRCDRV+++ +    +VT
Sbjct: 7  GKSSWPELVGKAGKEAEKTIERENPLVD-AIIVDEGSVVIQDFRCDRVWVWVDPKTAIVT 66

Query: 61 RTPHIG 66
          RTP IG
Sbjct: 67 RTPFIG 71

[Close]

BLAST of MC05g0055 vs. ExPASy TrEMBL
Match: A0A022QVV7 (Uncharacterized protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a019023mg PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.11e-15
Identity = 40/66 (60.61%), Postives = 47/66 (71.21%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GK +WPELV  P  AAAA I++EN LV  AIIV  G++V TDFRCDRV++F   NG  VT
Sbjct: 7  GKRSWPELVGVPGNAAAATIERENRLVN-AIIVPPGNSVITDFRCDRVWVFVNGNGN-VT 66

Query: 61 RTPHIG 66
          R P IG
Sbjct: 67 RVPSIG 70

[Close]

BLAST of MC05g0055 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 60.5 bits (145), Expect = 6.0e-10
Identity = 31/65 (47.69%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 2  KSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVTR 61
          K++WPEL  +    AA  I++ENP V  A+I+ +GS VT DFRCDRV +F + N  +V +
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVIL-DGSPVTADFRCDRVRVFVDGN-RIVVK 67

Query: 62 TPHIG 67
          TP  G
Sbjct: 68 TPKSG 70

[Close]

BLAST of MC05g0055 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.8e-06
Identity = 26/66 (39.39%), Postives = 42/66 (63.64%), Query Frame = 0


Query: 1  GKSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVT 60
          GKS+WPEL+ +  + A   I++ENP + KA+I+ +G+ V   F C RV+++    G +V 
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKM-KAVIILDGTVVPEIFICSRVYVWVNDCG-IVV 95

Query: 61 RTPHIG 67
          + P IG
Sbjct: 96 QIPIIG 99

[Close]

BLAST of MC05g0055 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 44.3 bits (103), Expect = 4.5e-05
Identity = 26/65 (40.00%), Postives = 36/65 (55.38%), Query Frame = 0


Query: 2  KSAWPELVWSPAKAAAAAIQKENPLVKKAIIVEEGSAVTTDFRCDRVFIFKEKNGELVTR 61
          K  WPELV    + A   I++ENP V  AI++  G     +F C+RVF++   NG+ V  
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNV-LAIVLRYGEKRIENFCCNRVFVYLGSNGQ-VAD 82

Query: 62 TPHIG 67
           P IG
Sbjct: 83 APMIG 85

The following BLAST results are available for this feature:

BLAST of MC05g0055 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P82381	2.0e-10	52.31	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
P24076	3.4e-10	53.03	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P19873	1.3e-09	53.03	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P80211	5.5e-08	47.69	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]
P16231	9.4e-08	49.15	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]

BLAST of MC05g0055 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN56903.1	1.19e-17	60.61	hypothetical protein Csa_010041 [Cucumis sativus]	[more]
XP_004134090.1	5.65e-16	57.58	glu S.griseus protease inhibitor [Cucumis sativus]	[more]
KAA0049293.1	1.62e-15	57.58	inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK1726...	[more]
KAA0049295.1	1.62e-15	57.58	inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK1726...	[more]
KAE8650342.1	2.91e-15	57.58	hypothetical protein Csa_009611 [Cucumis sativus]	[more]

BLAST of MC05g0055 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L593	5.76e-18	60.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142950 PE=3 SV=1	[more]
A0A0A0L795	2.74e-16	57.58	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142960 PE=3 SV=1	[more]
A0A5D3D0M4	7.84e-16	57.58	Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A5D3CZE2	7.84e-16	57.58	Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A022QVV7	3.11e-15	60.61	Uncharacterized protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a019023mg PE...	[more]

BLAST of MC05g0055 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	6.0e-10	47.69	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	1.8e-06	39.39	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	4.5e-05	40.00	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 30..41 score: 35.14 coord: 4..17 score: 47.83 coord: 42..52 score: 51.78
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 2..66 e-value: 1.9E-21 score: 76.5
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 1..66
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..66 e-value: 1.3E-22 score: 81.9
None	No IPR available	PANTHER	PTHR33091:SF77	INHIBITOR OF TRYPSIN AND HAGEMAN FACTOR-LIKE PROTEIN	coord: 1..66
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 1..66

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0055.1	MC05g0055.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity