MC05g0050 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0050
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProteinase inhibitor
LocationMC05: 399191 .. 399382 (+)
RNA-Seq ExpressionMC05g0050
SyntenyMC05g0050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTAAAACTTCGTGGCCGGAGCTGGTCGGAAAACTGGGGAAGGTGGCGGAGGAGAGGATTGAGAGAGAGCAGCCATGGACAAATGCTAAGATTGTTCAAGAAGGAAATTTCGTGACCACAGATTATAGGTGTGATAGGGTTTGGATTTGGGTCAATAAAAATGGCTTTGTTACTAGAATTCCCATTATTGGT

mRNA sequence

GGTAAAACTTCGTGGCCGGAGCTGGTCGGAAAACTGGGGAAGGTGGCGGAGGAGAGGATTGAGAGAGAGCAGCCATGGACAAATGCTAAGATTGTTCAAGAAGGAAATTTCGTGACCACAGATTATAGGTGTGATAGGGTTTGGATTTGGGTCAATAAAAATGGCTTTGTTACTAGAATTCCCATTATTGGT

Coding sequence (CDS)

GGTAAAACTTCGTGGCCGGAGCTGGTCGGAAAACTGGGGAAGGTGGCGGAGGAGAGGATTGAGAGAGAGCAGCCATGGACAAATGCTAAGATTGTTCAAGAAGGAAATTTCGTGACCACAGATTATAGGTGTGATAGGGTTTGGATTTGGGTCAATAAAAATGGCTTTGTTACTAGAATTCCCATTATTGGT

Protein sequence

GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRIPIIG
Homology
BLAST of MC05g0050 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.5e-13
Identity = 35/64 (54.69%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK SWP+LVG  G  A+  IERE P   A IV+ G+ VT D+RCDRV +WV + G V R 
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARP 64

Query: 61 PIIG 65
          P IG
Sbjct: 65 PAIG 68

BLAST of MC05g0050 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 5.5e-13
Identity = 32/63 (50.79%), Postives = 45/63 (71.43%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK +WPELVGK G +A   +ERE    +A +++EG+ +T D+RCDRVW+ VN +G VT +
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSV 65

Query: 61 PII 64
          P I
Sbjct: 66 PHI 68

BLAST of MC05g0050 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 5.5e-13
Identity = 36/64 (56.25%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK+SWP LVG  G VA+  IER+ P   A I++EG  VT D+RC+RV IWVNK G V   
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 64

Query: 61 PIIG 65
          P IG
Sbjct: 65 PRIG 68

BLAST of MC05g0050 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.3e-09
Identity = 29/61 (47.54%), Postives = 36/61 (59.02%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK  WPELVG+ G  A   IERE P   + +  E +  T D+RCDRVW+ V+  G V R 
Sbjct: 6  GKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGVVVRT 65

Query: 61 P 62
          P
Sbjct: 66 P 66

BLAST of MC05g0050 vs. ExPASy Swiss-Prot
Match: P81712 (Subtilisin inhibitor CLSI-I OS=Canavalia lineata OX=28957 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 7.0e-08
Identity = 29/63 (46.03%), Postives = 41/63 (65.08%), Query Frame = 0

Query: 2  KTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRIP 61
          KTSWPELVG   + A E+I+ E      ++V  G+FVT DY+  RV ++V+++  VTR P
Sbjct: 4  KTSWPELVGVTAEEA-EKIKEEMSGVEIQVVPPGSFVTADYKPQRVRLYVDESNKVTRTP 63

Query: 62 IIG 65
           IG
Sbjct: 64 GIG 65

BLAST of MC05g0050 vs. NCBI nr
Match: KAG7018770.1 (hypothetical protein SDJN02_20643, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 112 bits (279), Expect = 1.15e-30
Identity = 51/65 (78.46%), Postives = 55/65 (84.62%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGN-FVTTDYRCDRVWIWVNKNGFVTR 60
          GKTSWPELVGK GK AEE IERE  W NAK+VQEG   VT DYRCDRVW+WV+K+GFVTR
Sbjct: 6  GKTSWPELVGKHGKAAEETIEREVAWVNAKVVQEGTTLVTADYRCDRVWVWVDKHGFVTR 65

Query: 61 IPIIG 64
          IPIIG
Sbjct: 66 IPIIG 70

BLAST of MC05g0050 vs. NCBI nr
Match: XP_020966911.1 (glu S.griseus protease inhibitor-like [Arachis ipaensis] >XP_025677502.1 glu S.griseus protease inhibitor-like [Arachis hypogaea] >QHN78257.1 Glu S.griseus protease inhibitor [Arachis hypogaea] >RYQ91879.1 hypothetical protein Ahy_B09g097926 [Arachis hypogaea])

HSP 1 Score: 103 bits (256), Expect = 3.73e-27
Identity = 46/64 (71.88%), Postives = 51/64 (79.69%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK+SWPELVG  G VAE RIERE P  NA IV EG+ VTTDYRCDRVW+W+NK G V R+
Sbjct: 7  GKSSWPELVGVEGTVAEARIERENPLVNAIIVPEGSMVTTDYRCDRVWVWINKAGIVKRV 66

Query: 61 PIIG 64
          PIIG
Sbjct: 67 PIIG 70

BLAST of MC05g0050 vs. NCBI nr
Match: XP_020987874.1 (glu S.griseus protease inhibitor-like [Arachis duranensis] >QHO35154.1 Glu S.griseus protease inhibitor [Arachis hypogaea] >RYR38297.1 hypothetical protein Ahy_A09g043293 [Arachis hypogaea])

HSP 1 Score: 102 bits (255), Expect = 5.30e-27
Identity = 46/64 (71.88%), Postives = 51/64 (79.69%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK+SWPELVG  G VAE RIERE P  NA IV EG+ VTTDYRCDRVW+W+NK G V R+
Sbjct: 7  GKSSWPELVGFEGTVAEARIERENPLVNAIIVPEGSIVTTDYRCDRVWVWINKAGIVKRV 66

Query: 61 PIIG 64
          PIIG
Sbjct: 67 PIIG 70

BLAST of MC05g0050 vs. NCBI nr
Match: TQD78533.1 (hypothetical protein C1H46_035894 [Malus baccata])

HSP 1 Score: 97.8 bits (242), Expect = 5.10e-25
Identity = 44/64 (68.75%), Postives = 49/64 (76.56%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK SWPELVG  GK AE  IE E    NA IV++G+FVT DYRCDRVW+WVNKNG VT I
Sbjct: 7  GKNSWPELVGAKGKDAEAIIESENRLVNAVIVKKGSFVTMDYRCDRVWVWVNKNGIVTEI 66

Query: 61 PIIG 64
          P+IG
Sbjct: 67 PVIG 70

BLAST of MC05g0050 vs. NCBI nr
Match: TKY51888.1 (Proteinase inhibitor [Spatholobus suberectus])

HSP 1 Score: 97.1 bits (240), Expect = 1.03e-24
Identity = 42/64 (65.62%), Postives = 50/64 (78.12%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK+SWPELVG  G VAE  IERE P  NAKIV EG+ VT D+RCDRVW+W+NK+G V ++
Sbjct: 7  GKSSWPELVGVQGTVAEATIERENPLVNAKIVPEGSVVTLDFRCDRVWVWINKDGIVKQV 66

Query: 61 PIIG 64
          P IG
Sbjct: 67 PHIG 70

BLAST of MC05g0050 vs. ExPASy TrEMBL
Match: A0A444XQ64 (Glu S.griseus protease inhibitor OS=Arachis hypogaea OX=3818 GN=Ahy_B09g097926 PE=3 SV=1)

HSP 1 Score: 103 bits (256), Expect = 1.81e-27
Identity = 46/64 (71.88%), Postives = 51/64 (79.69%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK+SWPELVG  G VAE RIERE P  NA IV EG+ VTTDYRCDRVW+W+NK G V R+
Sbjct: 7  GKSSWPELVGVEGTVAEARIERENPLVNAIIVPEGSMVTTDYRCDRVWVWINKAGIVKRV 66

Query: 61 PIIG 64
          PIIG
Sbjct: 67 PIIG 70

BLAST of MC05g0050 vs. ExPASy TrEMBL
Match: A0A445BI04 (Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_A09g043293 PE=3 SV=1)

HSP 1 Score: 102 bits (255), Expect = 2.57e-27
Identity = 46/64 (71.88%), Postives = 51/64 (79.69%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK+SWPELVG  G VAE RIERE P  NA IV EG+ VTTDYRCDRVW+W+NK G V R+
Sbjct: 7  GKSSWPELVGFEGTVAEARIERENPLVNAIIVPEGSIVTTDYRCDRVWVWINKAGIVKRV 66

Query: 61 PIIG 64
          PIIG
Sbjct: 67 PIIG 70

BLAST of MC05g0050 vs. ExPASy TrEMBL
Match: A0A6P5MSW9 (glu S.griseus protease inhibitor-like OS=Arachis duranensis OX=130453 GN=LOC110275866 PE=3 SV=1)

HSP 1 Score: 102 bits (255), Expect = 2.57e-27
Identity = 46/64 (71.88%), Postives = 51/64 (79.69%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK+SWPELVG  G VAE RIERE P  NA IV EG+ VTTDYRCDRVW+W+NK G V R+
Sbjct: 7  GKSSWPELVGFEGTVAEARIERENPLVNAIIVPEGSIVTTDYRCDRVWVWINKAGIVKRV 66

Query: 61 PIIG 64
          PIIG
Sbjct: 67 PIIG 70

BLAST of MC05g0050 vs. ExPASy TrEMBL
Match: A0A540KWC4 (Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_035894 PE=3 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.47e-25
Identity = 44/64 (68.75%), Postives = 49/64 (76.56%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK SWPELVG  GK AE  IE E    NA IV++G+FVT DYRCDRVW+WVNKNG VT I
Sbjct: 7  GKNSWPELVGAKGKDAEAIIESENRLVNAVIVKKGSFVTMDYRCDRVWVWVNKNGIVTEI 66

Query: 61 PIIG 64
          P+IG
Sbjct: 67 PVIG 70

BLAST of MC05g0050 vs. ExPASy TrEMBL
Match: A0A7N2LVT1 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.99e-25
Identity = 43/64 (67.19%), Postives = 50/64 (78.12%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK SWPELVG  G VAE  IERE P  +A IV+EG+ V  D+RCDRVW+WV+KNG VTR+
Sbjct: 7  GKNSWPELVGVQGTVAEATIERENPSVDAIIVEEGSVVPQDFRCDRVWVWVDKNGIVTRV 66

Query: 61 PIIG 64
          PIIG
Sbjct: 67 PIIG 70

BLAST of MC05g0050 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 71.6 bits (174), Expect = 2.5e-13
Identity = 33/64 (51.56%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK+SWPEL+G  G+ A+E IERE P   A I+ +G  V   + C RV++WVN  G V +I
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 61 PIIG 65
          PIIG
Sbjct: 96 PIIG 99

BLAST of MC05g0050 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 64.3 bits (155), Expect = 4.0e-11
Identity = 30/63 (47.62%), Postives = 39/63 (61.90%), Query Frame = 0

Query: 2  KTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRIP 61
          K SWPEL G  G  A   IERE P  NA ++ +G+ VT D+RCDRV ++V+ N  V + P
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTP 67

Query: 62 IIG 65
            G
Sbjct: 68 KSG 70

BLAST of MC05g0050 vs. TAIR 10
Match: AT5G43570.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.5e-10
Identity = 30/61 (49.18%), Postives = 38/61 (62.30%), Query Frame = 0

Query: 2  KTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRIP 61
          K+SWPELVG+ G+  +E I+RE     AKI+ E   V     CDRV++ VN  G VTR P
Sbjct: 14 KSSWPELVGRRGEEVKEIIDRENTKVTAKIISENAVVLAVVICDRVYVRVNDQGIVTRTP 73

Query: 62 I 63
          I
Sbjct: 74 I 74

BLAST of MC05g0050 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.7e-09
Identity = 28/64 (43.75%), Postives = 36/64 (56.25%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK SWPEL+G  G  A   I+ E    N  +V +GN+VT D  C RV +WV++   V R 
Sbjct: 10 GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 69

Query: 61 PIIG 65
          P  G
Sbjct: 70 PTAG 73

BLAST of MC05g0050 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.7e-09
Identity = 28/64 (43.75%), Postives = 36/64 (56.25%), Query Frame = 0

Query: 1  GKTSWPELVGKLGKVAEERIEREQPWTNAKIVQEGNFVTTDYRCDRVWIWVNKNGFVTRI 60
          GK SWPEL+G  G  A   I+ E    N  +V +GN+VT D  C RV +WV++   V R 
Sbjct: 25 GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 84

Query: 61 PIIG 65
          P  G
Sbjct: 85 PTAG 88

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P240762.5e-1354.69Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1[more]
P823815.5e-1350.79Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1[more]
P198735.5e-1356.25Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
P802111.3e-0947.54Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1[more]
P817127.0e-0846.03Subtilisin inhibitor CLSI-I OS=Canavalia lineata OX=28957 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG7018770.11.15e-3078.46hypothetical protein SDJN02_20643, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_020966911.13.73e-2771.88glu S.griseus protease inhibitor-like [Arachis ipaensis] >XP_025677502.1 glu S.g... [more]
XP_020987874.15.30e-2771.88glu S.griseus protease inhibitor-like [Arachis duranensis] >QHO35154.1 Glu S.gri... [more]
TQD78533.15.10e-2568.75hypothetical protein C1H46_035894 [Malus baccata][more]
TKY51888.11.03e-2465.63Proteinase inhibitor [Spatholobus suberectus][more]
Match NameE-valueIdentityDescription
A0A444XQ641.81e-2771.88Glu S.griseus protease inhibitor OS=Arachis hypogaea OX=3818 GN=Ahy_B09g097926 P... [more]
A0A445BI042.57e-2771.88Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_A09g043293 PE=3 SV=1[more]
A0A6P5MSW92.57e-2771.88glu S.griseus protease inhibitor-like OS=Arachis duranensis OX=130453 GN=LOC1102... [more]
A0A540KWC42.47e-2568.75Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_035894 PE=3 SV=1[more]
A0A7N2LVT14.99e-2567.19Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G43580.12.5e-1351.56Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38870.14.0e-1147.62Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT5G43570.11.5e-1049.18Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.11.7e-0943.75Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.21.7e-0943.75Serine protease inhibitor, potato inhibitor I-type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRINTSPR00292POTATOINHBTRcoord: 4..17
score: 52.48
coord: 29..40
score: 28.62
coord: 41..51
score: 51.78
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 2..64
e-value: 4.3E-25
score: 88.2
IPR000864Proteinase inhibitor I13, potato inhibitor IPANTHERPTHR33091PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 1..64
IPR000864Proteinase inhibitor I13, potato inhibitor IPROSITEPS00285POTATO_INHIBITORcoord: 5..16
NoneNo IPR availableGENE3D3.30.10.10Trypsin Inhibitor V, subunit Acoord: 1..64
e-value: 1.0E-26
score: 95.1
NoneNo IPR availablePANTHERPTHR33091:SF41SERINE PROTEASE INHIBITOR, POTATO INHIBITOR I-TYPE FAMILY PROTEIN-RELATEDcoord: 1..64
IPR036354Proteinase inhibitor I13, potato inhibitor I superfamilySUPERFAMILY54654CI-2 family of serine protease inhibitorscoord: 1..64

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0050.1MC05g0050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0009611 response to wounding
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity