MC04g_new0598 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g_new0598
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	metalloendoproteinase 2-MMP-like
Location	MC04: 24136075 .. 24137148 (-)
RNA-Seq Expression	MC04g_new0598
Synteny	MC04g_new0598

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCATCAAAACTCTGCGCATTCCCATGGTTTGGTGTTTCTTAACCAACTTAGCGGATACCACAAGGGCGATAAAGGGGAAGGAATTCACCAGCTGAAGGAATACCTTCACCGTTTCGGCTACTTGAACAATGTTCAGTTTCATCCCGAAAATAATGACAATGAGTTTGACGAGTTCTTGGAATCTGCCATTAAAACCTACCAAATAAACTATAATCTCAAGGCCACAGGAACTATTGATGCCACGACGTTGGCTCAAATGTCAAAGCCTCGATGTGGGGTTGCAGATGTCGTTGATGGCAAGACTGGGATGAGATCTGGGAAGAAAATGAATCAACATAGAAAAATAAGTGGCCATTTCCGCACAGTTTCTCACTTCGCCTTCTTTGAAGGAAATCCTAGGTGGCCAGCTACTAAATCCCACCTCACTTACGGTAAATTTAATAGTTTATTCATATTCTAAATACTTTATGCCGGTTCCTACTCTATTCTTTGAAATTTAATTTTTTTTCCCTTATAAATAGGATTCCTCCCGGGAACCCCATCGGAGGCGATAAGTCCTGTGAGCCGAGCTTTTACGACATGGGCTGCGAACACTCACTTCACCTTCTCCCAAGCTTCTTCCTATCAAACGGCTGACATCAAAATAAGCTTTGAAAGTGGCGAGCACGGGGACGGTAATCCCTTCGATGGTGTCGGAGGAGTCATAGCCCATGCATTTTCACCCACAGATGGGAGGCTGCATTTCGACGCCGTTGAGCGTTGGGCGGTTGGCGCCGTTCCCGGTTCAAACGACGTGGAGACGGTGGCGTTGCATGAGATTGGACACCTTCTCGGCCTCCATCACAGCTCCGTTGAAGGGGCTATCATGTGGCCAACCATCATGCAAGGAGCTACCAAGGGTTTGCATGCCGATGACATTGCAGGCATAACGGCTTTGTACAATACTACTTCTTGAGTATGTTTGTGTATAATTTCGAAGAAAATTGGACTTAGAAAACTGCATTACAATCTGCAGTTAGAACAATTTTTTATTTATGAATCAATTTGTTATTCATTTTTATTTTCATAAAGAC

mRNA sequence

ATGTCAAAGCCTCGATGTGGGGTTGCAGATGTCGTTGATGGCAAGACTGGGATGAGATCTGGGAAGAAAATGAATCAACATAGAAAAATAAGTGGCCATTTCCGCACAGTTTCTCACTTCGCCTTCTTTGAAGGAAATCCTAGGTGGCCAGCTACTAAATCCCACCTCACTTACGGATTCCTCCCGGGAACCCCATCGGAGGCGATAAGTCCTGTGAGCCGAGCTTTTACGACATGGGCTGCGAACACTCACTTCACCTTCTCCCAAGCTTCTTCCTATCAAACGGCTGACATCAAAATAAGCTTTGAAAGTGGCGAGCACGGGGACGGTAATCCCTTCGATGGTGTCGGAGGAGTCATAGCCCATGCATTTTCACCCACAGATGGGAGGCTGCATTTCGACGCCGTTGAGCGTTGGGCGGTTGGCGCCGTTCCCGGTTCAAACGACGTGGAGACGGTGGCGTTGCATGAGATTGGACACCTTCTCGGCCTCCATCACAGCTCCGTTGAAGGGGCTATCATGTGGCCAACCATCATGCAAGGAGCTACCAAGGGTTTGCATGCCGATGACATTGCAGGCATAACGGCTTTGTACAATACTACTTCTTGA

Coding sequence (CDS)

ATGTCAAAGCCTCGATGTGGGGTTGCAGATGTCGTTGATGGCAAGACTGGGATGAGATCTGGGAAGAAAATGAATCAACATAGAAAAATAAGTGGCCATTTCCGCACAGTTTCTCACTTCGCCTTCTTTGAAGGAAATCCTAGGTGGCCAGCTACTAAATCCCACCTCACTTACGGATTCCTCCCGGGAACCCCATCGGAGGCGATAAGTCCTGTGAGCCGAGCTTTTACGACATGGGCTGCGAACACTCACTTCACCTTCTCCCAAGCTTCTTCCTATCAAACGGCTGACATCAAAATAAGCTTTGAAAGTGGCGAGCACGGGGACGGTAATCCCTTCGATGGTGTCGGAGGAGTCATAGCCCATGCATTTTCACCCACAGATGGGAGGCTGCATTTCGACGCCGTTGAGCGTTGGGCGGTTGGCGCCGTTCCCGGTTCAAACGACGTGGAGACGGTGGCGTTGCATGAGATTGGACACCTTCTCGGCCTCCATCACAGCTCCGTTGAAGGGGCTATCATGTGGCCAACCATCATGCAAGGAGCTACCAAGGGTTTGCATGCCGATGACATTGCAGGCATAACGGCTTTGTACAATACTACTTCTTGA

Protein sequence

MSKPRCGVADVVDGKTGMRSGKKMNQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIMQGATKGLHADDIAGITALYNTTS

Homology

[Close]

BLAST of MC04g_new0598 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.2e-44
Identity = 99/208 (47.60%), Postives = 118/208 (56.73%), Query Frame = 0


Query: 4   PRCGVADVVDGKTGMRSGKKMNQ--HRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFL 63
           PRCG  DVV+G + M SG+K  +         F  V H++FF G PRWP  +  LTY F 
Sbjct: 121 PRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFD 180

Query: 64  P--GTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 123
           P      E  S  SRAFT W   T  TF++   + T+DI I F SGEHGDG PFDG    
Sbjct: 181 PRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRT 240

Query: 124 IAHAFSPTDGRLHFDAVERWAVGAVPGSN--------DVETVALHEIGHLLGLHHSSVEG 183
           +AHAFSP  G  H D  E W V    G          D+E+VA+HEIGHLLGL HSSVEG
Sbjct: 241 LAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEG 300

Query: 184 AIMWPTIMQGATK-GLHADDIAGITALY 199
           +IM+PTI  G  K  L  DD+ G+  LY
Sbjct: 301 SIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of MC04g_new0598 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.2e-43
Identity = 97/206 (47.09%), Postives = 120/206 (58.25%), Query Frame = 0


Query: 4   PRCGVADVVDGKTGMRSGKKMNQHRKIS-GHFRTVSHFAFFEGNPRWPATKSHLTYGFLP 63
           PRCG  DVV+G + M  G++       S  H   V  +  F G PRWP  +  LTY F P
Sbjct: 118 PRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDP 177

Query: 64  GTP--SEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVI 123
             P   E  S  SRAF  W+  T   F+ + S+ T+DI I F +G+HGDG PFDGV G +
Sbjct: 178 KNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTL 237

Query: 124 AHAFSPTDGRLHFDAVERWAVG-------AVPGSNDVETVALHEIGHLLGLHHSSVEGAI 183
           AHAFSP  G+ H DA E W V        +V  + D+E+VA+HEIGHLLGL HSSVE +I
Sbjct: 238 AHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESI 297

Query: 184 MWPTIMQGATK-GLHADDIAGITALY 199
           M+PTI  G  K  L  DD+ GI  LY
Sbjct: 298 MYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of MC04g_new0598 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.0e-40
Identity = 99/208 (47.60%), Postives = 114/208 (54.81%), Query Frame = 0


Query: 3   KPRCGVADVVDGKTGMRSGKKMNQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLP 62
           KPRCG  D++DG + M  GK +          RT   ++FF G PRWP  K  LTY F P
Sbjct: 116 KPRCGNPDLIDGVSEMNGGKIL----------RTTEKYSFFPGKPRWPKRKRDLTYAFAP 175

Query: 63  --GTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVI 122
                 E     SRAFT WA  T   F+++ S   ADI I F SGEHGDG PFDG  G +
Sbjct: 176 QNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTL 235

Query: 123 AHAFSPTDGRLHFDAVERWAVG---------AVPGSNDVETVALHEIGHLLGLHHSSVEG 182
           AHA SP  G LH D  E W +           V    D+E+VA+HEIGHLLGL HSSVE 
Sbjct: 236 AHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVED 295

Query: 183 AIMWPTIMQGATK-GLHADDIAGITALY 199
           AIM+P I  G  K  L  DDI GI  LY
Sbjct: 296 AIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of MC04g_new0598 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 157.5 bits (397), Expect = 1.6e-37
Identity = 90/204 (44.12%), Postives = 117/204 (57.35%), Query Frame = 0


Query: 4   PRCGVADVVDGKTGMRSGKKMNQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLPG 63
           PRCGV D++           +N ++  S  F  +S + FF+  PRW A  + LTY F P 
Sbjct: 111 PRCGVPDII-----------INTNKTTS--FGMISDYTFFKDMPRWQAGTTQLTYAFSPE 170

Query: 64  TPSEAI--SPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVIA 123
              +    S ++RAF+ W    +  F + +SY+TA+IKI F S  HGD  PFDG GG++ 
Sbjct: 171 PRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILG 230

Query: 124 HAFSPTDGRLHFDAVERWAVGA------VPGSNDVETVALHEIGHLLGLHHSSVEGAIMW 183
           HAF+PTDGR HFDA E W          V  + D+E+VA+HEIGHLLGL HSS   AIM+
Sbjct: 231 HAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMY 290

Query: 184 PTIMQGATK-GLHADDIAGITALY 199
           P+I     K  L  DDI GI  LY
Sbjct: 291 PSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]

BLAST of MC04g_new0598 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 5.5e-35
Identity = 87/209 (41.63%), Postives = 113/209 (54.07%), Query Frame = 0


Query: 4   PRCGVADVVDGKTGMRSGKKMNQHRKISGHFRTVSHFAFFEGNPRWPA-TKSHLTYGFLP 63
           PRCG  D V+ KT                 F T   + +F G PRW       LTY F  
Sbjct: 104 PRCGFPDDVEPKT---------------APFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQ 163

Query: 64  GTPSEAISPVS------RAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGV 123
              +  ++P        RAF  WA+    +F +   Y  ADIKI F +G+HGDG PFDGV
Sbjct: 164 ENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGV 223

Query: 124 GGVIAHAFSPTDGRLHFDAVERWAVGAVPGSN----DVETVALHEIGHLLGLHHSSVEGA 183
            GV+AH FSP +GRLH D  E WAV      +    D+E+VA+HEIGH+LGL HSSV+ A
Sbjct: 224 LGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDA 283

Query: 184 IMWPTIMQGATK-GLHADDIAGITALYNT 201
            M+PT+   + K  L+ DD+ G+ +LY T
Sbjct: 284 AMYPTLKPRSKKVNLNMDDVVGVQSLYGT 297

[Close]

BLAST of MC04g_new0598 vs. NCBI nr
Match: XP_038889209.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 371 bits (952), Expect = 3.09e-127
Identity = 176/203 (86.70%), Postives = 191/203 (94.09%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYG 60
           MSKPRCG+ADVVDGKT M+SGKKM NQHRKI+GHF +VSH+AFFEGNP+WPA+KSHLTYG
Sbjct: 113 MSKPRCGIADVVDGKTMMKSGKKMVNQHRKINGHFHSVSHYAFFEGNPKWPASKSHLTYG 172

Query: 61  FLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 120
           FLPGTPSEA++PV RAFTTWAANTHF+FSQAS+Y+TADIKISFESG+HGDG+ FDGVGGV
Sbjct: 173 FLPGTPSEAVAPVGRAFTTWAANTHFSFSQASAYETADIKISFESGDHGDGHAFDGVGGV 232

Query: 121 IAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 180
           IAHAFSPTDGRLHFDAVE WA+GAV  S DVETVALHEIGHLLGLHHSSVEGAIMWPTIM
Sbjct: 233 IAHAFSPTDGRLHFDAVEPWAIGAVADSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 292

Query: 181 QGATKGLHADDIAGITALYNTTS 202
            GATKGLHADDIAGI ALY TTS
Sbjct: 293 GGATKGLHADDIAGINALYTTTS 315

[Close]

BLAST of MC04g_new0598 vs. NCBI nr
Match: XP_022993647.1 (metalloendoproteinase 2-MMP-like [Cucurbita maxima])

HSP 1 Score: 365 bits (936), Expect = 8.40e-125
Identity = 174/203 (85.71%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYG 60
           MSKPRCGVADV  GKTGM SGKKM N+HRKIS HF  VSHFAFF+GNP+WPA+KSHL YG
Sbjct: 113 MSKPRCGVADVFHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYG 172

Query: 61  FLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 120
           FLPGTP EAI+PV RAFTTWAANTHFTFSQASSY+TADIKISFESG+HGDG  FDGVGGV
Sbjct: 173 FLPGTPIEAIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGV 232

Query: 121 IAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 180
           IAHAFSPTDGRLHFDA+E WA+GAVP S D+ETVALHEIGHLLGLHHSSVEGAIMWPTIM
Sbjct: 233 IAHAFSPTDGRLHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIM 292

Query: 181 QGATKGLHADDIAGITALYNTTS 202
           +GATKGLHADDIAGI ALY  TS
Sbjct: 293 EGATKGLHADDIAGINALYTATS 315

[Close]

BLAST of MC04g_new0598 vs. NCBI nr
Match: XP_023551500.1 (metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362 bits (928), Expect = 1.34e-123
Identity = 174/203 (85.71%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYG 60
           MSKPRCGVADV  GKT M SGKKM N+HRKI G F  VSHFAFF+GNP+WPA+KSHLTYG
Sbjct: 112 MSKPRCGVADVFHGKTRMNSGKKMVNRHRKIGGRFHGVSHFAFFDGNPKWPASKSHLTYG 171

Query: 61  FLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 120
           FLPGTP+EAI+PV RAFTTWAANTHFTFSQASSY+TADIKISFESG+HGDG  FDGVGGV
Sbjct: 172 FLPGTPTEAIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGV 231

Query: 121 IAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 180
           IAHAFSPTDGRLHFDAVE WA+GAVP S D+ETVALHEIGHLLGL HSSVEGAIMWPTIM
Sbjct: 232 IAHAFSPTDGRLHFDAVEPWAIGAVPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIM 291

Query: 181 QGATKGLHADDIAGITALYNTTS 202
           +GATKGLHADDIAGI ALY TTS
Sbjct: 292 EGATKGLHADDIAGINALYTTTS 314

[Close]

BLAST of MC04g_new0598 vs. NCBI nr
Match: KAG7016165.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 361 bits (926), Expect = 2.79e-123
Identity = 173/203 (85.22%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYG 60
           MSKPRCGVADV  GKT M SGKKM N+HRKISG F  VSHFAFF+GNP+WPA+KSHLTYG
Sbjct: 113 MSKPRCGVADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYG 172

Query: 61  FLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 120
           FLPGTP+EAI+PV RAFTTWAANTHFTFS ASSY+TADIKISFESG+HGDG  FDGVGGV
Sbjct: 173 FLPGTPTEAIAPVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGV 232

Query: 121 IAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 180
           IAHAFSPTDGRLHFDAVE WA+GA+P S D+ETVALHEIGHLLGL HSSVEGAIMWPTIM
Sbjct: 233 IAHAFSPTDGRLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIM 292

Query: 181 QGATKGLHADDIAGITALYNTTS 202
           +GATKGLHADDIAGI ALY TTS
Sbjct: 293 EGATKGLHADDIAGINALYTTTS 315

[Close]

BLAST of MC04g_new0598 vs. NCBI nr
Match: XP_022938357.1 (metalloendoproteinase 2-MMP-like [Cucurbita moschata])

HSP 1 Score: 361 bits (926), Expect = 2.79e-123
Identity = 173/203 (85.22%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYG 60
           MSKPRCGVADV  GKT M SGKKM N+HRKISG F  VSHFAFF+GNP+WPA+KSHLTYG
Sbjct: 113 MSKPRCGVADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYG 172

Query: 61  FLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 120
           FLPGTP+EAI+PV RAFTTWAANTHFTFS ASSY+TADIKISFESG+HGDG  FDGVGGV
Sbjct: 173 FLPGTPTEAIAPVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGV 232

Query: 121 IAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 180
           IAHAFSPTDGRLHFDAVE WA+GA+P S D+ETVALHEIGHLLGL HSSVEGAIMWPTIM
Sbjct: 233 IAHAFSPTDGRLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIM 292

Query: 181 QGATKGLHADDIAGITALYNTTS 202
           +GATKGLHADDIAGI ALY TTS
Sbjct: 293 EGATKGLHADDIAGINALYTTTS 315

[Close]

BLAST of MC04g_new0598 vs. ExPASy TrEMBL
Match: A0A6J1JTF5 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=3 SV=1)

HSP 1 Score: 365 bits (936), Expect = 4.07e-125
Identity = 174/203 (85.71%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYG 60
           MSKPRCGVADV  GKTGM SGKKM N+HRKIS HF  VSHFAFF+GNP+WPA+KSHL YG
Sbjct: 113 MSKPRCGVADVFHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYG 172

Query: 61  FLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 120
           FLPGTP EAI+PV RAFTTWAANTHFTFSQASSY+TADIKISFESG+HGDG  FDGVGGV
Sbjct: 173 FLPGTPIEAIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGV 232

Query: 121 IAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 180
           IAHAFSPTDGRLHFDA+E WA+GAVP S D+ETVALHEIGHLLGLHHSSVEGAIMWPTIM
Sbjct: 233 IAHAFSPTDGRLHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIM 292

Query: 181 QGATKGLHADDIAGITALYNTTS 202
           +GATKGLHADDIAGI ALY  TS
Sbjct: 293 EGATKGLHADDIAGINALYTATS 315

[Close]

BLAST of MC04g_new0598 vs. ExPASy TrEMBL
Match: A0A6J1FDT9 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 PE=3 SV=1)

HSP 1 Score: 361 bits (926), Expect = 1.35e-123
Identity = 173/203 (85.22%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYG 60
           MSKPRCGVADV  GKT M SGKKM N+HRKISG F  VSHFAFF+GNP+WPA+KSHLTYG
Sbjct: 113 MSKPRCGVADVFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYG 172

Query: 61  FLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 120
           FLPGTP+EAI+PV RAFTTWAANTHFTFS ASSY+TADIKISFESG+HGDG  FDGVGGV
Sbjct: 173 FLPGTPTEAIAPVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGV 232

Query: 121 IAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 180
           IAHAFSPTDGRLHFDAVE WA+GA+P S D+ETVALHEIGHLLGL HSSVEGAIMWPTIM
Sbjct: 233 IAHAFSPTDGRLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIM 292

Query: 181 QGATKGLHADDIAGITALYNTTS 202
           +GATKGLHADDIAGI ALY TTS
Sbjct: 293 EGATKGLHADDIAGINALYTTTS 315

[Close]

BLAST of MC04g_new0598 vs. ExPASy TrEMBL
Match: A0A6J1FFZ1 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111445135 PE=4 SV=1)

HSP 1 Score: 346 bits (887), Expect = 5.89e-119
Identity = 167/203 (82.27%), Postives = 181/203 (89.16%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYG 60
           MSKPRCGVAD++DGK+GM+SGK M NQHRK SGHF  VSHF FF+GN +WP +KSHLTYG
Sbjct: 28  MSKPRCGVADIIDGKSGMKSGKNMVNQHRK-SGHFHQVSHFTFFKGNLKWPNSKSHLTYG 87

Query: 61  FLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 120
           FLPGTPS A+ PV RAFTTWAANTHF+FS ASSY+TADIKISFE G+HGDG PFD VGGV
Sbjct: 88  FLPGTPSGAVDPVGRAFTTWAANTHFSFSLASSYKTADIKISFERGDHGDGFPFDNVGGV 147

Query: 121 IAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 180
           IAHAF+PTDGRLHFDAVERW  GAVP S DVETVALHEIGHLLGL+HSSVEGAIMWPTIM
Sbjct: 148 IAHAFAPTDGRLHFDAVERWTDGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPTIM 207

Query: 181 QGATKGLHADDIAGITALYNTTS 202
           +GATKGLHADDIAGI ALY  TS
Sbjct: 208 RGATKGLHADDIAGIKALYTPTS 229

[Close]

BLAST of MC04g_new0598 vs. ExPASy TrEMBL
Match: A0A0A0KS47 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 348 bits (894), Expect = 9.29e-119
Identity = 165/202 (81.68%), Postives = 181/202 (89.60%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKK-MNQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYG 60
           MSKPRCGVAD+++GKT M+SGK+ +NQHRKISGHF  VSHFAFFEGN RWP +KSHLTYG
Sbjct: 112 MSKPRCGVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYG 171

Query: 61  FLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 120
           FLPGTPSEAISPV+RAFTTWAANTHF+FSQ S Y+ ADIKISFE G+HGDG PFD VGGV
Sbjct: 172 FLPGTPSEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGV 231

Query: 121 IAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 180
           +AHAF+PTDGRLHFDAVE WA GAVP S D+ETVALHEIGHLLGLHHSSVEGAIMWP+IM
Sbjct: 232 LAHAFAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIM 291

Query: 181 QGATKGLHADDIAGITALYNTT 201
            GATKGLHADDI GI  LY T+
Sbjct: 292 GGATKGLHADDIEGIKVLYTTS 313

[Close]

BLAST of MC04g_new0598 vs. ExPASy TrEMBL
Match: A0A5A7T7T1 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00140 PE=3 SV=1)

HSP 1 Score: 344 bits (882), Expect = 2.15e-117
Identity = 160/202 (79.21%), Postives = 181/202 (89.60%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKKM-NQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYG 60
           MSKPRCGVAD+++G+TGM+SGK++ N+HRKI GHF  VSHFAFFEGN +WP +KSHLTYG
Sbjct: 81  MSKPRCGVADIINGRTGMKSGKRLVNRHRKIIGHFHEVSHFAFFEGNLKWPNSKSHLTYG 140

Query: 61  FLPGTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 120
           FLPGTPSEAISPV+RAFTTWAANTHF+FSQ S+Y+ ADIK+SFE G+HGDG PFD VGGV
Sbjct: 141 FLPGTPSEAISPVNRAFTTWAANTHFSFSQESNYENADIKVSFERGDHGDGFPFDSVGGV 200

Query: 121 IAHAFSPTDGRLHFDAVERWAVGAVPGSNDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 180
           +AHAF+PTDGRLHFDAVE WA GAVP S D+ETVALHEIGHLLGLHHSSVEGAIMWP+IM
Sbjct: 201 LAHAFAPTDGRLHFDAVEHWADGAVPNSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIM 260

Query: 181 QGATKGLHADDIAGITALYNTT 201
            G TKGLHADDI GI  LY T+
Sbjct: 261 GGTTKGLHADDIEGIKVLYTTS 282

[Close]

BLAST of MC04g_new0598 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 180.3 bits (456), Expect = 1.6e-45
Identity = 99/208 (47.60%), Postives = 118/208 (56.73%), Query Frame = 0


Query: 4   PRCGVADVVDGKTGMRSGKKMNQ--HRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFL 63
           PRCG  DVV+G + M SG+K  +         F  V H++FF G PRWP  +  LTY F 
Sbjct: 121 PRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFD 180

Query: 64  P--GTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGV 123
           P      E  S  SRAFT W   T  TF++   + T+DI I F SGEHGDG PFDG    
Sbjct: 181 PRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRT 240

Query: 124 IAHAFSPTDGRLHFDAVERWAVGAVPGSN--------DVETVALHEIGHLLGLHHSSVEG 183
           +AHAFSP  G  H D  E W V    G          D+E+VA+HEIGHLLGL HSSVEG
Sbjct: 241 LAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEG 300

Query: 184 AIMWPTIMQGATK-GLHADDIAGITALY 199
           +IM+PTI  G  K  L  DD+ G+  LY
Sbjct: 301 SIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of MC04g_new0598 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 176.0 bits (445), Expect = 3.0e-44
Identity = 97/206 (47.09%), Postives = 120/206 (58.25%), Query Frame = 0


Query: 4   PRCGVADVVDGKTGMRSGKKMNQHRKIS-GHFRTVSHFAFFEGNPRWPATKSHLTYGFLP 63
           PRCG  DVV+G + M  G++       S  H   V  +  F G PRWP  +  LTY F P
Sbjct: 118 PRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDP 177

Query: 64  GTP--SEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVI 123
             P   E  S  SRAF  W+  T   F+ + S+ T+DI I F +G+HGDG PFDGV G +
Sbjct: 178 KNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTL 237

Query: 124 AHAFSPTDGRLHFDAVERWAVG-------AVPGSNDVETVALHEIGHLLGLHHSSVEGAI 183
           AHAFSP  G+ H DA E W V        +V  + D+E+VA+HEIGHLLGL HSSVE +I
Sbjct: 238 AHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESI 297

Query: 184 MWPTIMQGATK-GLHADDIAGITALY 199
           M+PTI  G  K  L  DD+ GI  LY
Sbjct: 298 MYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of MC04g_new0598 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 167.2 bits (422), Expect = 1.4e-41
Identity = 99/208 (47.60%), Postives = 114/208 (54.81%), Query Frame = 0


Query: 3   KPRCGVADVVDGKTGMRSGKKMNQHRKISGHFRTVSHFAFFEGNPRWPATKSHLTYGFLP 62
           KPRCG  D++DG + M  GK +          RT   ++FF G PRWP  K  LTY F P
Sbjct: 116 KPRCGNPDLIDGVSEMNGGKIL----------RTTEKYSFFPGKPRWPKRKRDLTYAFAP 175

Query: 63  --GTPSEAISPVSRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGVGGVI 122
                 E     SRAFT WA  T   F+++ S   ADI I F SGEHGDG PFDG  G +
Sbjct: 176 QNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTL 235

Query: 123 AHAFSPTDGRLHFDAVERWAVG---------AVPGSNDVETVALHEIGHLLGLHHSSVEG 182
           AHA SP  G LH D  E W +           V    D+E+VA+HEIGHLLGL HSSVE 
Sbjct: 236 AHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVED 295

Query: 183 AIMWPTIMQGATK-GLHADDIAGITALY 199
           AIM+P I  G  K  L  DDI GI  LY
Sbjct: 296 AIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of MC04g_new0598 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 152.9 bits (385), Expect = 2.7e-37
Identity = 89/211 (42.18%), Postives = 119/211 (56.40%), Query Frame = 0


Query: 1   MSKPRCGVADVVDGKTGMRSGKKMNQHRKISGHF-RTVSHFAFFEGNPRWPATKSHLTYG 60
           MS PRCGV+D                H  I+  F  T +H+ +F G P+W   +  LTY 
Sbjct: 125 MSLPRCGVSDT---------------HMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYA 184

Query: 61  F-----LPGTPSEAISPV-SRAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPF 120
                 L    SE +  V  RAF+ W++    +F +   + TAD+KI F +G+HGDG PF
Sbjct: 185 ISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPF 244

Query: 121 DGVGGVIAHAFSPTDGRLHFDAVERWAV-----GAVPGSNDVETVALHEIGHLLGLHHSS 180
           DGV G +AHAF+P +GRLH DA E W V     G+   + D+E+VA HEIGHLLGL HSS
Sbjct: 245 DGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSS 304

Query: 181 VEGAIMWPTIMQGATK-GLHADDIAGITALY 199
            E A+M+P++     K  L  DD+AG+  LY
Sbjct: 305 QESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of MC04g_new0598 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 149.1 bits (375), Expect = 3.9e-36
Identity = 87/209 (41.63%), Postives = 113/209 (54.07%), Query Frame = 0


Query: 4   PRCGVADVVDGKTGMRSGKKMNQHRKISGHFRTVSHFAFFEGNPRWPA-TKSHLTYGFLP 63
           PRCG  D V+ KT                 F T   + +F G PRW       LTY F  
Sbjct: 104 PRCGFPDDVEPKT---------------APFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQ 163

Query: 64  GTPSEAISPVS------RAFTTWAANTHFTFSQASSYQTADIKISFESGEHGDGNPFDGV 123
              +  ++P        RAF  WA+    +F +   Y  ADIKI F +G+HGDG PFDGV
Sbjct: 164 ENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGV 223

Query: 124 GGVIAHAFSPTDGRLHFDAVERWAVGAVPGSN----DVETVALHEIGHLLGLHHSSVEGA 183
            GV+AH FSP +GRLH D  E WAV      +    D+E+VA+HEIGH+LGL HSSV+ A
Sbjct: 224 LGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDA 283

Query: 184 IMWPTIMQGATK-GLHADDIAGITALYNT 201
            M+PT+   + K  L+ DD+ G+ +LY T
Sbjct: 284 AMYPTLKPRSKKVNLNMDDVVGVQSLYGT 297

The following BLAST results are available for this feature:

BLAST of MC04g_new0598 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XF51	2.2e-44	47.60	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
O04529	4.2e-43	47.09	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q9ZUJ5	2.0e-40	47.60	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
P29136	1.6e-37	44.12	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q8GWW6	5.5e-35	41.63	Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1	[more]

BLAST of MC04g_new0598 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889209.1	3.09e-127	86.70	metalloendoproteinase 2-MMP-like [Benincasa hispida]	[more]
XP_022993647.1	8.40e-125	85.71	metalloendoproteinase 2-MMP-like [Cucurbita maxima]	[more]
XP_023551500.1	1.34e-123	85.71	metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo]	[more]
KAG7016165.1	2.79e-123	85.22	Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_022938357.1	2.79e-123	85.22	metalloendoproteinase 2-MMP-like [Cucurbita moschata]	[more]

BLAST of MC04g_new0598 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JTF5	4.07e-125	85.71	metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=...	[more]
A0A6J1FDT9	1.35e-123	85.22	metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 P...	[more]
A0A6J1FFZ1	5.89e-119	82.27	metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111445135 P...	[more]
A0A0A0KS47	9.29e-119	81.68	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 S...	[more]
A0A5A7T7T1	2.15e-117	79.21	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of MC04g_new0598 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24140.1	1.6e-45	47.60	Matrixin family protein	[more]
AT1G70170.1	3.0e-44	47.09	matrix metalloproteinase	[more]
AT1G59970.1	1.4e-41	47.60	Matrixin family protein	[more]
AT4G16640.1	2.7e-37	42.18	Matrixin family protein	[more]
AT2G45040.1	3.9e-36	41.63	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 153..178 score: 57.69 coord: 186..199 score: 53.22 coord: 72..87 score: 34.91 coord: 96..124 score: 62.03 coord: 1..11 score: 54.01
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 45..200 e-value: 5.2E-30 score: 115.7
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 52..198 e-value: 5.2E-44 score: 149.9
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 1..202 e-value: 6.9E-58 score: 198.0
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 1..199
None	No IPR available	PANTHER	PTHR10201:SF269	METALLOENDOPROTEINASE 3-MMP-LIKE	coord: 1..199
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 42..199
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 48..198 e-value: 2.68748E-59 score: 181.248

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g_new0598.1	MC04g_new0598.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity